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phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of sanguibacter gen.nov.16s rrna gene sequencing studies were performed on some gram-positive coryneform bacteria of unknown taxonomic position isolated from blood and milk of healthy cows. comparative sequence analysis demonstrated that the milk isolates corresponded to oerskovia xanthineolytica whereas those from blood consisted of two distinct, albeit highly related species, within the family cellulomonadaceae. based on the phylogenetic and phenotypic distinctiveness of the blood isolates, it is proposed that they b ...19957765903
sanguibacter inulinus sp. nov.six strains of coryneform bacteria were isolated from blood samples obtained from healthy cows. phenotypic and molecular genetic studies showed that these isolates represent a new species of the genus sanguibacter, for which the name sanguibacter inulinus is proposed. the type strain of s. inulinus is strain st-50 (= ncfb 3024).19968782694
clinical microbiology of coryneform bacteria.coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ...19978993861
identification of a chitinase-producing bacterium c4 and histopathologic study on locusts.in order to develop the potential of chitinase-producing micro-organisms as biocontrol agents for insect pests, five chitinase-producing bacterial strains (c1, c2, c3, c4 and c5) previously isolated from soil samples were chosen to infect grassland locusts. the data showed that the mortality rate of locusts fed with strain c4 was significantly higher than that of other groups, and its pathogenicity was confirmed by koch's law. midgut tissues of locusts infected with c4 were examined with a light ...200515619732
phenotypic and genetic characterization of clinical isolates of cdc coryneform group a-3: proposal of a new species of cellulomonas, cellulomonas denverensis sp. nov.cdc coryneform group a-3 bacteria are rare human pathogens. in this study, six group a-3 isolates (two from blood, one from cerebrospinal fluid, and one each from homograft valve, lip wound, and pilonidal cyst) were compared to the type strains of phenotypically related organisms, cellulomonas fimi, cellulomonas hominis, oerskovia turbata, and sanguibacter suarezii, and characterized by phenotypic, chemotaxonomic, and genotypic studies. dna-dna reassociation analysis identified two genomic group ...200515814993
sanguibacter marinus sp. nov., isolated from coastal sediment.a gram-positive, coryneform bacterium, strain 1-19(t), was isolated from coastal sediment from the eastern china sea. phylogenetic analysis based on 16s rrna gene sequences showed that the organism formed a robust clade with the type species of the genus sanguibacter and displayed less than 97% gene sequence similarity. phenotypic characteristics supported the assignment of this organism to the genus sanguibacter. a range of biochemical features distinguished it from all other sanguibacter speci ...200516166662
cloning and expression of a chitinase gene from sanguibacter sp. c4.the chitinase chi58 is an extracellular chitinase produced by sanguibacter sp.strain c4. the gene-specific pcr primers were used to detect the presence of the chia gene in strain c4. a chia fragment (chia-f) was amplified from the c4 genomic dna and was used to blast-search the related sequences from the genbank database. by alignment and selection of the highly conserved regions of the homologous sequences, two pairs of primers were designed to amplify the open reading frame (orf) of the chitin ...200617112976
sanguibacter antarcticus sp. nov., isolated from antarctic sea sand.a gram-positive, yellow-pigmented bacterium, strain kopri 21702(t), was isolated from sea sand on king george island, antarctica. cells were irregular rods with peritrichous flagella; their optimum growth temperature was 23-26 degrees c. phylogenetic analysis based on 16s rrna gene sequences revealed that the antarctic isolate formed a distinct phyletic line in a clade of the genus sanguibacter and showed highest sequence similarity (97.7%) to the type strain of sanguibacter keddieii. the major ...200818175681
sanguibacter soli sp. nov., isolated from soil of a ginseng field.a gram-positive, non-spore-forming, rod-shaped, motile bacterium, designated strain dcy22(t), was isolated from soil of a ginseng field in south korea and characterized in order to determine its taxonomic position. 16s rrna gene sequence analysis revealed that strain dcy22(t) belonged within the family sanguibacteraceae, and highest levels of sequence similarity were found with sanguibacter marinus 1-19(t) (96.8 %), sanguibacter suarezii st-26(t) (96.0 %), sanguibacter inulinus st-50(t) (95.9 %) ...200818319451
occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland.the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ...200919572487
expression of recombinant endochitinase from the antarctic bacterium, sanguibacter antarcticus kopri 21702 in pichia pastoris by codon optimization.an endochitinase was previously purified and the gene was cloned from the psychrophilic antarctic bacterium, sanguibacter antarcticus (kctc 13143). in the present study, recombinant endochitinase, rchi21702, was expressed using a yeast expression system (pichia pastoris) and codon optimization. the expressed rchi21702 was purified by phenyl-sepharose column chromatography. optimal expression yielded 1-mg purified enzyme from 1-l bioreactor culture. when p-np-(glcnac)(2) was used as a substrate, ...201020100576
isolation and characterization of folate-producing bacteria from oat bran and rye flakes.the aim of this research was to identify endogenous bacteria in commercial oat bran and rye flake products in order to study their folate production capability while maintaining the soluble dietary fibre components in physiologically active, unhydrolyzed form. fourty-two bacteria were isolated from three different oat bran products and 26 bacteria from one rye flake consumer product. the bacteria were tentatively identified by sequence analysis of the 16s rrna genes. the identification results r ...201020678822
optimization of cold-active chitinase production from the antarctic bacterium, sanguibacter antarcticus kopri 21702.in the present study, cultivation conditions and medium components were optimized using statistical design and analysis to enhance the production of chi21702, a cold-active extracellular chitinase from the antarctic bacterium sanguibacter antarcticus kopri 21702. identification of significant carbon sources and other key elements was performed using a statistical design technique. chitin and glycerol were selected as main carbon sources, and the ratio of complex nitrogen sources to carbon source ...201020922383
complete genome sequence of sanguibacter keddieii type strain (st-74).sanguibacter keddieii is the type species of the genus sanguibacter, the only genus within the family of sanguibacteraceae. phylogenetically, this family is located in the neighborhood of the genus oerskovia and the family cellulomonadaceae within the actinobacterial suborder micrococcineae. the strain described in this report was isolated from blood of apparently healthy cows. here we describe the features of this organism, together with the complete genome sequence, and annotation. this is the ...200921304646
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