Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [an epidemiological investigation of bats carrying sars-cov in guangzhou and its vicinity]. | to detect serve acute respiratory syndrome-associated coronavirus (sars-cov) and sars-like-cov in fruit bats captured in guangzhou and its vicinity. | 2006 | 16864084 |
| pleistocene climatic cycling drives intra-specific diversification in the intermediate horseshoe bat (rhinolophus affinis) in southern china. | the repeated formation and loss of land-bridges during the pleistocene have had lasting impacts on population genetic structure. in the tropics, where island populations persisted through multiple glacial cycles, alternating periods of isolation and contact are expected to have driven population and taxonomic divergence. here, we combine mitochondrial and nuclear sequence data with microsatellites to dissect the impact of pleistocene climate change on intra-specific diversification in the horses ... | 2010 | 20561192 |
| historical introgression and the persistence of ghost alleles in the intermediate horseshoe bat (rhinolophus affinis). | phylogenetic conflicts between genetic markers can help to disentangle complex histories of phylogeography and introgression among taxa. we previously proposed that the chinese mainland subspecies of the intermediate horseshoe bat rhinolophus affinis himalayanus colonized hainan island to form the subspecies r. a. hainanus. subsequent recolonization of the mainland formed a third taxon, r. a macrurus, and a secondary contact zone with the ancestral himalayanus. to test for historical and recurre ... | 2013 | 23205821 |
| phylogeny and origins of hantaviruses harbored by bats, insectivores, and rodents. | hantaviruses are among the most important zoonotic pathogens of humans and the subject of heightened global attention. despite the importance of hantaviruses for public health, there is no consensus on their evolutionary history and especially the frequency of virus-host co-divergence versus cross-species virus transmission. documenting the extent of hantavirus biodiversity, and particularly their range of mammalian hosts, is critical to resolving this issue. here, we describe four novel hantavi ... | 2013 | 23408889 |
| prevalence and genetic characterization of toxoplasma gondii infection in bats in southern china. | toxoplasma gondii can infect a wide variety of warm-blooded animals, including bats. limited information on t. gondii infection in bats is available in china. the objective of the present study was to determine prevalence and genetic diversity of t. gondii infection in bats in southern china. a total of 608 bats representing 12 species, including 120 aselliscus stoliczkanus, 59 myotis chinensis, 11 miniopterus schreibersii, 53 rhinolophus affinis, 32 rhinolophus pusillus, 81 hipposideros armiger ... | 2014 | 24813744 |
| differential introgression among loci across a hybrid zone of the intermediate horseshoe bat (rhinolophus affinis). | hybrid zones formed by the secondary contact of divergent lineages represent natural laboratories for studying the genetic basis of speciation. here we tested for patterns of differential introgression among three x-linked and 11 autosomal regions to identify candidate loci related to either reproductive isolation or adaptive introgression across a hybrid zone between two chinese mainland subspecies of the intermediate horseshoe bat rhinolophus affinis: r. a. himalayanus and r. a. macrurus. | 2014 | 25011626 |
| molecular characterization of rig-i, stat-1 and ifn-beta in the horseshoe bat. | wild chinese horseshoe bats have been proven to be natural reservoirs of sars-like coronaviruses. however, the molecular characterization of key proteins in bats still needs to be explored further. in this study, we used cloning and bioinformatics to analyze the sequence of rig-i, stat-1 and ifn-β in the immortalized cell lines from rhinolophus affinis and rhinolophus sinicus. then, we treated different bat cells, mouse embryonic fibroblasts (mef) and splenocytes with polyinosinic-polycytidylic ... | 2015 | 25680291 |
| discovery of a novel putative atypical porcine pestivirus in pigs in the usa. | pestiviruses are some of the most significant pathogens affecting ruminants and swine. here, we assembled a 11 276 bp contig encoding a predicted 3635 aa polyprotein from porcine serum with 68 % pairwise identity to that of a recently partially characterized rhinolophus affinis pestivirus (rapv) and approximately 25-28 % pairwise identity to those of other pestiviruses. the virus was provisionally named atypical porcine pestivirus (appv). metagenomic sequencing of 182 serum samples identified fo ... | 2015 | 26219947 |
| coexistence of multiple coronaviruses in several bat colonies in an abandoned mineshaft. | since the 2002-2003 severe acute respiratory syndrome (sars) outbreak prompted a search for the natural reservoir of the sars coronavirus, numerous alpha- and betacoronaviruses have been discovered in bats around the world. bats are likely the natural reservoir of alpha- and betacoronaviruses, and due to the rich diversity and global distribution of bats, the number of bat coronaviruses will likely increase. we conducted a surveillance of coronaviruses in bats in an abandoned mineshaft in mojian ... | 2016 | 26920708 |
| description of a new tick species, ixodes collaris n. sp. (acari: ixodidae), from bats (chiroptera: hipposideridae, rhinolophidae) in vietnam. | in a recent study on ixodid bat ticks from eurasia, a high genetic difference was found between ixodes vespertilionis from europe and vietnam. accordingly, it was proposed that i. vespertilionis is a species complex, with at least one additional, hitherto undescribed species. the aim of the present study was to investigate the morphology of bat ticks from vietnam and to assess their taxonomic status in comparison with those collected in europe. | 2016 | 27286701 |
| detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in china. | in recent years, novel hepadnaviruses, hepeviruses, hepatoviruses, and hepaciviruses have been discovered in various species of bat around the world, indicating that bats may act as natural reservoirs for these hepatitis viruses. in order to further assess the distribution of hepatitis viruses in bat populations in china, we tested the presence of these hepatitis viruses in our archived bat liver samples that originated from several bat species and various geographical regions in china. | 2017 | 28222808 |
| identification of diverse bat alphacoronaviruses and betacoronaviruses in china provides new insights into the evolution and origin of coronavirus-related diseases. | outbreaks of severe acute respiratory syndrome (sars) in 2002, middle east respiratory syndrome in 2012 and fatal swine acute diarrhea syndrome in 2017 caused serious infectious diseases in humans and in livestock, resulting in serious public health threats and huge economic losses. all such coronaviruses (covs) were confirmed to originate from bats. to continuously monitor the epidemic-related covs in bats, virome analysis was used to classify covs from 831 bats of 15 species in yunnan, guangxi ... | 2019 | 31474969 |
| taxonomic implications of geographical variation in rhinolophus affinis (chiroptera: rhinolophidae) in mainland southeast asia. | rhinolophusaffinis sensu lato isdistributed throughout southeast asia. the taxonomic status of forms attributed to the species is unclear due to the limited sample size with incomplete datasets and the taxa have high variation in morphology and echolocation call frequency. the aim of the study was to evaluate the distribution and taxonomic status of the subspecific forms of r. affinis in mainland southeast asia using large sample size with multiple datasets, including morphological, acoustic, an ... | 2015 | 31966118 |
| transmission routes of 2019-ncov and controls in dental practice. | a novel β-coronavirus (2019-ncov) caused severe and even fetal pneumonia explored in a seafood market of wuhan city, hubei province, china, and rapidly spread to other provinces of china and other countries. the 2019-ncov was different from sars-cov, but shared the same host receptor the human angiotensin-converting enzyme 2 (ace2). the natural host of 2019-ncov may be the bat rhinolophus affinis as 2019-ncov showed 96.2% of whole-genome identity to batcov ratg13. the person-to-person transmissi ... | 2020 | 32127517 |
| genome-wide data reveal discordant mitonuclear introgression in the intermediate horseshoe bat (rhinolophus affinis). | closely related taxa often exhibit mitonuclear discordance attributed to introgression of mitochondrial dna (mtdna), yet few studies have considered the underlying causes of mtdna introgression. here we test for demographic versus adaptive processes as explanations for mtdna introgression in three subspecies of the intermediate horseshoe bat (rhinolophus affinis). we generated sequences of 1692 nuclear genes and 13 mitochondrial protein-coding genes for 48 individuals. phylogenetic reconstructio ... | 2020 | 32534185 |
| an update on the origin of sars-cov-2: despite closest identity, bat (ratg13) and pangolin derived coronaviruses varied in the critical binding site and o-linked glycan residues. | the initial cases of severe acute respiratory syndrome coronavirus-2 (sars-cov-2) occurred in wuhan, china, in december 2019 and swept the world by 23 june 2020 with 8 993 659 active cases, 469 587 deaths across 216 countries, areas or territories. this strongly implies global transmission occurred before the lockdown of china. however, the initial source's transmission routes of sars-cov-2 remain obscure and controversial. research data suggest bat (ratg13) and pangolin carried cov were the pro ... | 2020 | 32633815 |
| mutation patterns of human sars-cov-2 and bat ratg13 coronavirus genomes are strongly biased towards c>u transitions, indicating rapid evolution in their hosts. | the pandemic caused by the spread of sars-cov-2 has led to considerable interest in its evolutionary origin and genome structure. here, we analyzed mutation patterns in 34 human sars-cov-2 isolates and a closely related ratg13 isolated from rhinolophus affinis (a horseshoe bat). we also evaluated the cpg dinucleotide contents in sars-cov-2 and other human and animal coronavirus genomes. out of 1136 single nucleotide variations (~4% divergence) between human sars-cov-2 and bat ratg13, 682 (60%) c ... | 2020 | 32646049 |
| echolocation call frequency variation in horseshoe bats: molecular basis revealed by comparative transcriptomics. | recently diverged taxa with contrasting phenotypes offer opportunities for unravelling the genetic basis of phenotypic variation in nature. horseshoe bats are a speciose group that exhibit a derived form of high-duty cycle echolocation in which the inner ear is finely tuned to echoes of the narrowband call frequency. here, by focusing on three recently diverged subspecies of the intermediate horseshoe bat (rhinolophus affinis) that display divergent echolocation call frequencies, we aim to ident ... | 2020 | 32900318 |
| understanding the dynamics of covid-19; implications for therapeutic intervention, vaccine development and movement control. | the covid-19 disease is caused by the recently identified sars-cov-2 virus, thought to have originated in bats (rhinolophus affinis), the virus being highly infective within the human population and spread by respiratory droplets, contaminated surfaces and close person-to-person contact. the virus is now pandemic and widely disseminated to almost every continent and nation with globally over twenty-seven million infections and over ninety-thousand reported deaths attributed to the covid-19 disea ... | 2020 | 32942955 |
| transcriptome sequencing of cochleae from constant-frequency and frequency-modulated echolocating bats. | echolocating bats are fascinating for their ability to 'see' the world in the darkness. ultrahigh frequency hearing is essential for echolocation. in this study we collected cochlear tissues from constant-frequency (cf) bats (two subspecies of rhinolophus affinis, rhinolophidae) and frequency-modulated (fm) bats (myotis ricketti, vespertilionidae) and applied pacbio single-molecule real-time isoform sequencing (iso-seq) technology to generate the full-length (fl) transcriptomes for the three tax ... | 2020 | 33051458 |
| conservation analysis of sars-cov-2 spike suggests complicated viral adaptation history from bat to human. | the current coronavirus disease 2019 (covid-19) pandemic, caused by severe acute respiratory syndrome (sars)-cov-2, has become the most devastating public health emergency in the 21st century and one of the most influential plagues in history. studies on the origin of sars-cov-2 have generally agreed that the virus probably comes from bat, closely related to a bat cov named bcov-ratg13 taken from horseshoe bat (rhinolophus affinis), with malayan pangolin (manis javanica) being a plausible interm ... | 2020 | 33372198 |
| bats, pangolins, minks and other animals - villains or victims of sars-cov-2? | coronavirus disease-19 (covid-19) is caused by the severe acute respiratory syndrome coronavirus-2 (sars-cov-2), which has become unstoppable, spreading rapidly worldwide and, consequently, reaching a pandemic level. this review aims to provide the information available so far on the likely animal origin of sars-cov-2 and its possible hosts/reservoirs as well as all natural animal infections and experimental evidence using animal models. horseshoe bats from the species rhinolophus affinis seem t ... | 2021 | 33464439 |
| the rhinolophus affinis bat ace2 and multiple animal orthologs are functional receptors for bat coronavirus ratg13 and sars-cov-2. | bat coronavirus (cov) ratg13 shares the highest genome sequence identity with severe acute respiratory syndrome coronavirus 2 (sars-cov-2) among all known coronaviruses, and also uses human angiotensin converting enzyme 2 (hace2) for virus entry. thus, sars-cov-2 is thought to have originated from bat. however, whether sars-cov-2 emerged from bats directly or through an intermediate host remains elusive. here, we found that rhinolophus affinis bat ace2 (raace2) is an entry receptor for both sars ... | 2021 | 33495713 |
| can ace2 receptor polymorphism predict species susceptibility to sars-cov-2? | a novel severe acute respiratory syndrome coronavirus, sars-cov-2, emerged in china in december 2019 and spread worldwide, causing more than 1.3 million deaths in 11 months. similar to the human sars-cov, sars-cov-2 shares strong sequence homologies with a sarbecovirus circulating in rhinolophus affinis bats. because bats are expected to be able to transmit their coronaviruses to intermediate animal hosts that in turn are a source of viruses able to cross species barriers and infect humans (so-c ... | 2021 | 33643982 |
| functional and genetic analysis of viral receptor ace2 orthologs reveals a broad potential host range of sars-cov-2. | the pandemic of covid-19, caused by sars-cov-2, is a major global health threat. epidemiological studies suggest that bats (rhinolophus affinis) are the natural zoonotic reservoir for sars-cov-2. however, the host range of sars-cov-2 and intermediate hosts that facilitate its transmission to humans remain unknown. the interaction of coronavirus with its host receptor is a key genetic determinant of host range and cross-species transmission. sars-cov-2 uses angiotensin-converting enzyme 2 (ace2) ... | 2021 | 33658332 |
| binding and molecular basis of the bat coronavirus ratg13 virus to ace2 in humans and other species. | severe acute respiratory syndrome coronavirus 2 (sars-cov-2) has been spreading worldwide, causing a global pandemic. bat-origin ratg13 is currently the most phylogenetically related virus. here we obtained the complex structure of the ratg13 receptor binding domain (rbd) with human ace2 (hace2) and evaluated binding of ratg13 rbd to 24 additional ace2 orthologs. by substituting residues in the ratg13 rbd with their counterparts in the sars-cov-2 rbd, we found that residue 501, the major positio ... | 2021 | 34139177 |
| inferring the ecological niche of bat viruses closely related to sars-cov-2 using phylogeographic analyses of rhinolophus species. | the severe acute respiratory syndrome coronavirus 2 (sars-cov-2) is the causal agent of the coronavirus disease 2019 (covid-19) pandemic. to date, viruses closely related to sars-cov-2 have been reported in four bat species: rhinolophus acuminatus, rhinolophus affinis, rhinolophus malayanus, and rhinolophus shameli. here, we analysed 343 sequences of the mitochondrial cytochrome c oxidase subunit 1 gene (co1) from georeferenced bats of the four rhinolophus species identified as reservoirs of vir ... | 2021 | 34253798 |
| identification of a novel lineage bat sars-related coronaviruses that use bat ace2 receptor. | severe respiratory disease coronavirus-2 (sars-cov-2) has been the most devastating disease covid-19 in the century. one of the unsolved scientific questions of sars-cov-2 is the animal origin of this virus. bats and pangolins are recognized as the most probable reservoir hosts that harbour highly similar sars-cov-2 related viruses (sarsr-cov-2). this study identified a novel lineage of sarsr-covs, including ratg15 and seven other viruses, from bats at the same location where we found ratg13 in ... | 2021 | 34263709 |