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rickettsiella phytoseiuli and virus-like particles in phytosfiulus persimilis (gamasoidea: phytoseiidae) mites.electron microscope investigation of tissue of phytoseiulus persimilis mites revealed its double infection with rickettsiella phytoseiuli and virus-like particles. the presence of rickettsiae in tissues of the mite seemed to be decisive for establishment of virus infection.197829476
[immunologic comparison between some wolbachiae and research of antigentic community with other members of the order of rickettsiales].the antigenic relationship between some rickettsiae of arthropods, which do not transmit germs pathogenic to vertebrates was investigated by agglutination and immunofluorescence techniques. the comparison of r. melolonthae, r. tipulae, r. cetonidarum, r. grylli and of the rickettsiae of the scorpion buthus occitanus shows that the wolbachiae are divided into three serological groups. the insect crystallogen rickettsiae belong to the first group, r. grylli and the scorpion rickettsia are antigeni ...197553109
[an ultrastructural study of the transformation of elementary bodies of rickettsiella into initial bodies (author's transl)].an intermediate stage between the elementary and the initial bodies of the rickettsietta genus is defined as the beginning of an intracellular cycle. it is characterized by several structural changes in the dense elementary body: the cytoplasm becomes less electron-dense; thus, the nucleoid and the ribosomes are visible. the inner layer of the cell-wall becomes progressively clearer and the trilamellar structure of the inner and outer membranes appears distinctly. 'preinitial body' is proposed a ...1979488269
[study of a "rickettsiella" (rickettsia) pathogen of the spider "pisaura mirabilis" (author's transl)].a rickettsiella-like organism has been described in the spider pisaura mirabilis cl., and placed into the genus rickettsiella on the basis of its cycle and ultrastructure. a chlamydial cycle takes place in the digestive cells and becomes more complex in the interstitial cells where giant bodies appear. the latter have been considered up to now as caracteristic of the genus rickettsiella but, in fact, seem to be in relation with the metabolism of the parasitized cell. consequently their presence ...1977869405
[cytochemical and freeze-etching studies of the ultrastructure of a rickettsiella in a crustacean (author's transl)].several characteristics of the intracellular cycle of ricketsiella are elucidated, particularly regarding the envelope and the cytoplasm of dense forms (elementary bodies) as well as the proteinic inclusions of giant bodies. cleavage faces have been displayed by freeze-etching, both at the level of cell wall and of plasma membrane. their characteristics approach those of gram-negative bacteria and are pratically common to all the stages. appearance and significance of a polysaccharidic cement jo ...1977931256
coxiella and rickettsiella: comparison of ultrastructure with special reference to their envelope.ultrastructure of rickettsiella phytoseiuli (r.p.) multiplying in female ticks dermacentor reticulatus was compared with that of coxiella burnetii (c.b.) in the same ticks and in mice. c.b. in ticks and mice were always represented by 2 main cell types: small dense round or rod-like cells (dc) and larger bacteria-like cells (bc). dc were surrounded with a characteristic five-layered 20 nm thick envelope. under the envelope dc had a stack of parallel intracytoplasmic membranes with a periodicity ...19911687641
[a trial of cultivating rickettsiella phytoseiuli on the sm imv-72 medium used for growing phytopathogenic mycoplasmas].the work presents data on growing rickettsiella phytoseiuli in the sm imv-72 medium which is used for cultivation of phytopathogenic mycoplasmas. rickettsiella was observed till the 66th day in the primary cultures and till the 16th day after the first passage. binary division of the cells has been found only in the primary cultures; a complex cycle of the rickettsiella development ceased at the stage of formation of the crystal-forming cells. attempts to passivate rickettsiella were failure.19911819757
mixed infection of rickettsiella phytoseiuli and coxiella burnetii in dermacentor reticulatus female ticks: electron microscope study.mixed infection of rickettsiella phytoseiuli and coxiella burnetii was investigated in hemolymph and organs of experimentally infected females of dermacentor reticulatus ticks. following intracoelomic infection, both agents, with the exception of gene's organ, multiplied well in the cells of the tick host's organs. two out of six developmental stages of r. phytoseiuli, i.e., crystal-forming and small dark particles, in dual infection with c. burnetii revealed marked morphological alterations. c. ...19902351845
virus-like particles in dermacentor reticulatus ticks.virus-like particles were found in the brain and salivary glands of dermacentor reticulatus ticks double infected with coxiella burnetii and rickettsiella phytoseiuli. the particles were spherical (diameter of about 70 nm) and consisted of an electron-dense inner core and a dense external coat.19892576601
pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera chlamydia, rickettsiella, and porochlamydia.the chromosome length of obligate intracellular procaryotes was determined by pulsed-field gel electrophoresis of intact or noti- and sfii-restricted genomes. sizes averaged 2,100, 1,720, 1,550, 2,650, and 1,450 kilobases for rickettsiella grylli, rickettsiella melolonthae, porochlamydia buthi, porochlamydia chironomi, and chlamydia psittaci and chlamydia trachomatis, respectively. an sfii restriction map of the r. melolonthae genome was derived.19892753864
experimental infection with rickettsiella phytoseiuli in adult female dermacentor reticulatus (ixodidae): an electron microscopy study.rickettsiella phytoseiuli naturally occurring in phytoseiulus persimilis mites was cultivated in adult female dermacentor reticulatus ticks. it demonstrates all six known developmental stages: dense, intermediate, bacterial, giant, crystal-forming and small dark particles. these stages of rickettsiae were found in salivary glands, malpighian tubules, synganglion, ovaries, tracheal complex, haemolymph, fat body and alimentary tract. rickettsiella phytoseiuli did not infect the gené's organ. it mu ...19892806015
electron microscopic study of developmental stages of rickettsiella phytoseiuli in phytoseiulus persimilis athias-henriot (gamasoidea:phytoseiidae) mites.rickettsiella phytoseiuli was found in great amounts in all tissues except of the nervous system of adult phytoseiulus persimilis mites. six morphologically different stages (dense, intermediate, bacterial, giant, crystal-forming and small dark particles) of r. phytoseiuli were detected. no rickettsiae were seen in the larvae and in phase 1 and 2 nymphae of these mites.19882897771
attempt to cultivate rickettsiella phytoseiuli in dermacentor reticulatus ticks: an electron microscopic study.rickettsiella phytoseiuli occurring in phytoseiulus persimilis mites was cultivated in dermacentor reticulatus ticks. rickettsiella multiplied similarly as in mites exerting all six developmental stages: dense, intermediate, bacterial, giant, crystal-forming and small dark particles.19882897779
behavioral fever and therapy in a rickettsia-infected orthoptera.gryllus bimaculatus were infected with an intracellular prokaryote, rickettsiella grylli, then reared either at fixed temperatures or in a temperature gradient (22-36 degrees c) where they could select the temperature they preferred. only 50% of the infected insects reared at 28 degrees c or less survived after 20 days, against 75% of those reared at 30 degrees c or more and 90% of those in the temperature gradient. examination of smears of insect tissue showed that all (100%) of the infected in ...19863755009
[experimental infection of mice by an entomopathogenic rickettsia: rickettsiella grylli. infestation by the intraperitoneal route. chronic phase]. 19863777788
[experimental infection of mice by entomopathogenic rickettsia: rickettsiella grylli. infestation by the intraperitoneal route. acute phase]. 19853835835
[effect of invertebrate rickettsiae on vertebrates: experimental infection of mice by rickettsiella grylli].an analysis by cytology, electron microscopy, and serology of experimental infections of the mouse by an invertebrate rickettsia with chlamydial cycle, rickettsiella grylli, showed that, according to the routes and the severity of the infection, the pathogen can have no effect, or cause local inflammations, or induce degenerative or malignant processes. the very limited development of the pathogen as well as the possible direct role of the chlamydial elementary bodies and toxins in the pathogene ...19853919886
[demonstration and comparison of antigens extracted from giant forms of 3 rickettsiella philip]. 19714107122
[characteristics of infection of melolontha melolontha l. larvea by rickettsiella melolonthae krieg]. 19734799123
"rickettsiella chironomi": an unusual bacterial pathogen which reproduces by multiple cell division. 19725044282
[on the experimental infection of gryllus bimaculatus and g. capitatus (orthoptera) by rickettsiella grylli, rickettsiosis agent in orthoptera]. 19685753287
comparison of the ultrastructure of small dense forms of chlamydiae and coxiella burnetii.in the course of passaging of coxiella burnetii (c.b.) in alveonasus lahorensis ticks, the haemocytes contained cell forms with electrondense cytoplasm, intracytoplasmic lamellar membranes, and a peculiar limiting membrane--25 to 30 nm thick "envelope complex". similar small forms occurred when c.b. had been cultured in the yolk sack of chick embryos. the dense forms of c.b. were similar to those of rickettsiella cells. dense forms (elementary bodies) surrounded by an "envelope complex" were fou ...19836135336
rickettsiaceae and chlamydiaceae: comparative electron microscopic studies.the structure and cytopathology of obligate intracellular bacteria belonging to families rickettsiaceae and chlamydiaceae and their interaction with eukaryotic host cells were compared in electron microscopic studies. "rickettsia-like" and "chlamydia-like" types of organization of bacterial cells and their interaction with host cells are presented. the rickettsia-like type is characterized by short rod-shaped cells multiplying freely ( extravacuolarly ) in the cytoplasm or nucleoplasm of the hos ...19846145350
reproduction and morphogenesis of rickettsiella chironomi, an unusual intracellular procaryotic parasite of midge larvae.the reproduction and morphogenesis of rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. the morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diameter), (ii) large spherical initial bodies (1.5-2 micrometers), (iii) spherical intermediate bodies (600 to 700 nm), and (iv) disk-shaped elementary bodies (60 x 600 nm). the primar ...19807204340
surface projections of a chlamydia-like parasite of midge larvae.projections are described in a chlamydia-like organism similar to rickettsiella chironomi. these projections were present only on the edge of the disk-like elementary bodies; rod-shaped, they were found to be composed of repetitive subunits.19807440504
phenomenon of rickettsiella phytoseiuli in phytoseiulus persimilis mite.an unknown microorganism occurring in a predaceous mite phytoseiulus persimilis was described by the author in 1977 as a new species rickettsiella phytoseiuli. some new results on the relation between this agent and its hosts are presented in this paper.19947866725
taxonomic studies of rickettsiella, rickettsia, and chlamydia using genomic dna.invertebrate pathogens of the genus rickettsiella (order rickettsiales) undergo a developmental cycle more characteristic of chlamydial organisms (order chlamydiales) than of typical rickettsiae. moreover, among recognized species of rickettsiella, there is considerable variation in host range and in the ultrastructure and development of the infectious stage, i.e., the elementary body. to begin an analysis of the taxonomic relationships of the invertebrate pathogens belonging to the genus ricket ...19947912708
use of partially engorged female ticks as laboratory animals in microbiological research.partially engorged female ticks were used as laboratory animals in microbiological research. the ticks, which were inoculated intracoelomally, became a convenient substrate for the detection of viruses, rickettsiae and protozoal parasites. this research concerned the isolation of newly recovered micro-organisms, the study of development, structure and distribution of microbial agents in ticks, and the study of their interaction with other pathogens or symbionts during mixed infection in a tick b ...19948025326
histopathological investigations on rickettsiella-like sp. and nonoccluded viruses infecting the pecan weevil curculio caryae, the squash beetle epilachna borealis, and the mexican bean beetle epilachna varivestiselectron microscopic observations of abnormal specimens of pecan weevils, curculio caryae, squash beetles, epilachna borealis, and mexican bean beetles, epilachna varivestis, obtained from insect-rearing facilities and from field collections revealed that rickettsia-like organisms (rlos) and viruses caused disease in these species. several pleomorphic forms of the rlos which were similar to those of rickettsiella sp. were found in tissues of the fat body, hypodermis, tracheal matrix, muscle, and ...19979056462
reassessment of the taxonomic position of rickettsiella grylli.we determined the 16s rrna gene sequence of rickettsiella grylli, an intracellular parasite of gryllus bimaculatus and related species of crickets. phylogenetic inferences made from alignment of this sequence with the sequences of other bacteria demonstrated that r. grylli is most closely related to coxiella burnetii and legionella species in the gamma subclass of the phylum proteobacteria. r. grylli was previously thought to be related to members of the order rickettsiales, but the representati ...19979336939
the specificity of behavioral fever in the cricket acheta domesticus.when infected, some insects can raise their body temperature by moving to warmer areas. this behavioral fever response can help the host overcome infection. however, not all parasites and pathogens are equally susceptible to increases in host temperature. elevating the temperature of the cricket acheta domesticus from room temperature (22 c) to 33 c did not reduce the survival of parasitoid flies or reduce the number of gregarine gut protozoans, and crickets infested with these parasites showed ...19989645851
morphological description of bacterial infection of digestive glands in the terrestrial isopod porcellio scaber (isopoda, crustacea)morphological studies of the hepatopancreas of the terrestrial isopod porcellio scaber revealed bacterial infection. the percentage of infected animals collected from the same site varied from 0 to 10% during the 4 years of study. transmission electron microscopy, scanning electron microscopy, and light microscopy revealed that infected glands differed from those in healthy isopods. the most prominent sign was white spots between 100 and 200 &mgr;m in diameter along the entire gland. these spots ...19999878297
ultrastructure and cytopathology of a rickettsia-like organism causing systemic infection in the redclaw crayfish, cherax quadricarinatus (crustacea: decapoda), in ecuador.a study of the ultrastructural characteristics of an intracellular bacterium infecting the redclaw crayfish, cherax quadricarinatus, a pathogen referred to previously as a rickettsia-like organism (rlo), revealed the presence of different developmental stages. these included a rod-shaped and uniformly electron-dense elementary body (eb) and an intermediate body (ib). the length of the eb varied between 0.48 and 0.6 microm, and the diameter was 0.3 microm. the ib was 0.75 to 1.1 microm long by 0. ...200011023732
light and electron microscopic study of a rickettsiella species from the cockroach blatta orientalis.an infection with rickettsiella sp. was responsible for an illness causing heavy body swelling in the oriental cockroach blatta orientalis. reproduction of the colony stagnated. vacuoles with parasitic bacteria occurred mainly in the fat body, but also in nearly all other organs, such as gut epithelium, malpighian tubules, blood cells, and ovarioles. the parasites clearly differed from the symbiotic bacteria of the genus blattabacterium, which regularly occur in the mycetocytes of b. orientalis. ...200011112369
rickettsiella-like bacteria in ixodes woodi (acari: ixodidae).we examined a parthenogenetic strain of the hard tick ixodes woodi bishopp for the presence of endosymbiotic bacteria. electron microscopic examination revealed the ovarian tissues and malpighian tubules were infected with pleomorphic bacteria. two basic types were observed: a larger granular cell and a smaller condensed cell. cloning and sequence analysis of polymerase chain reaction (pcr) amplified 16s rrna gene yielded a single sequence from bacteria present in i. woodi tissues. phylogenetic ...200212061452
application of 16s rdna-dgge to examine the microbial ecology associated with a social wasp vespula germanica.invertebrates host numerous bacteria, with interactions ranging from pathogenesis to symbiosis. while certain symbiotic relationships have been well studied, little is known about the dynamics of these bacterial communities. denaturing gradient gel electrophoresis (dgge) was used to examine the bacterial microflora associated with the eusocial wasp vespula germanica. dgge profiles of larval guts revealed a variable microflora, suggesting that v. germanica is not dependent on a particular suite o ...200312542639
a rickettsial disease of larvae of species of stethorus caused by rickettsiella stethorae, n. sp. 195713475267
gamma-proteobacteria aquicella lusitana gen. nov., sp. nov., and aquicella siphonis sp. nov. infect protozoa and require activated charcoal for growth in laboratory media.several isolates, belonging to two new species of the same novel genus of gamma-proteobacteria, were recovered from drilled well (borehole) and spa water at são gemil in central portugal. these organisms are phylogenetically most closely related to the strictly intracellular uncultured species of the genus rickettsiella, which cause disease in arthropods, and to the facultatively intracellular species of the genus legionella, some of which cause legionnaires' disease and pontiac fever. the são g ...200314602611
genome analysis of coxiella burnetii species: insights into pathogenesis and evolution and implications for biodefense.coxiella burnetii, the etiological agent of q fever, is a class b biodefense agent. we are continuing the momentum of discovery generated by the first coxiella genome sequences by extending the breadth of genomics to include four additional heterogeneous c. burnetii strains. we are also sequencing the genome of rickettsiella grylli, an intracellular parasite of grasshoppers and the closest known phylogenetic relative to the coxiella group. these data will enable the investigation of fundamental ...200516481558
'candidatus rhabdochlamydia crassificans', an intracellular bacterial pathogen of the cockroach blatta orientalis (insecta: blattodea).the genus rickettsiella comprises various intracellular bacterial pathogens of arthropods, exhibiting a chlamydia-like developmental cycle. species may be divided into two main groups, the r. popilliae-r. grylli group and the r. chironomi group. previous phylogenetic studies based on the 16s ribosomal rna encoding gene showed that two rickettsiella species, one from each group, belong in reality to two distantly related lineages, the gamma-proteobacteria (r. grylli) and the chlamydiales ('candid ...200716934426
bacterial symbionts in the hepatopancreas of isopods: diversity and environmental transmission.the midgut glands (hepatopancreas) of terrestrial isopods contain bacterial symbionts. we analysed the phylogenetic diversity of hepatopancreatic bacteria in isopod species from various suborders colonizing marine, semiterrestrial, terrestrial and freshwater habitats. hepatopancreatic bacteria were absent in the marine isopod idotea balthica (valvifera). the symbiotic bacteria present in the midgut glands of the freshwater isopod asellus aquaticus (asellota) were closely related to members of th ...200717506824
molecular characterization and evolution of arthropod-pathogenic rickettsiella bacteria.we determined the 16s rrna gene sequences of three crustacean "rickettsiella armadillidii" strains. rickettsiella bacteria overall appear to form a monophyletic group that diverged from coxiella bacteria approximately 350 million years ago. therefore, the genus rickettsiella as a whole (not just rickettsiella grylli) should be classified among the gammaproteobacteria instead of the alphaproteobacteria.200717557851
type iv secretion system components as phylogenetic markers of entomopathogenic bacteria of the genus rickettsiella.the genus rickettsiella (class gammaproteobacteria; order legionellales; family coxiellaceae) comprises intracellular bacterial pathogens of a wide range of arthropods that are currently classified in the three recognized species, rickettsiella popilliae, rickettsiella grylli, and rickettsiella chironomi. rickettsiella bacteria contain a type ivb secretion system (t4ss) known to be a key virulence factor of the related genus legionella. providing the first respective sequence information for the ...200818179586
genetic and electron-microscopic characterization of rickettsiella tipulae, an intracellular bacterial pathogen of the crane fly, tipula paludosa.rickettsiella tipulae is an intracellular bacterial pathogen of larvae of the crane fly, tipula paludosa (diptera: tipulidae) and has previously been claimed to represent an independent species within the genus rickettsiella. recently, this taxon has been reorganized and transferred as a whole from the alpha-proteobacterial order rickettsiales to the gamma-proteobacterial order legionellales. here we present the electron-microscopic identification of this rickettsial pathogen together with the f ...200818374354
16s rrna-, groel- and mucz-based assessment of the taxonomic position of 'rickettsiella melolonthae' and its implications for the organization of the genus rickettsiella.'rickettsiella melolonthae' is an intracellularly multiplying bacterial pathogen of european cockchafers, melolontha melolontha (linnaeus, 1758) and melolontha hippocastani (fabricius, 1801) (coleoptera: scarabaeidae). we report the first determination of nucleotide sequences from this organism, i.e. the 16s rrna encoding rrs gene, the chaperonin encoding groel gene and the mucz gene encoding the orthologue of a capsule synthesis-inducing factor of coxiella burnetii. within the genus rickettsiel ...200818398164
whole genome-based assessment of the taxonomic position of the arthropod pathogenic bacterium rickettsiella grylli.rickettsiella grylli is an intracellular bacterial pathogen of aquatic and terrestrial arthropods. previous determination of its 16s rrna-encoding sequence has led to the taxonomic classification of the genus rickettsiella in the class gammaproteobacteria, order legionellales, family coxiellaceae, i.e. in close vicinity to vertebrate pathogenic bacteria of the genera coxiella and legionella. here we use the additional information available from the recently published first whole genome sequence ...200818422627
reorganization and monophyly of the genus rickettsiella: all in good time. 200818695072
natural enemies of mass-reared predatory mites (family phytoseiidae) used for biological pest control.predatory mites of the family phytoseiidae are valued natural enemies that provide effective pest control in greenhouses and on agricultural crops. mass-reared phytoseiids are occasionally associated with microorganisms and although their effects are not always apparent, some are pathogenic and reduce host fitness. invertebrate pathogens are encountered more frequently in mass production systems than in nature because rearing environments often cause overcrowding and other stresses that favour p ...200818726699
bacterial taxa associated with the hematophagous mite dermanyssus gallinae detected by 16s rrna pcr amplification and ttge fingerprinting.dermanyssus gallinae (arthropoda, mesostigmata) is suspected to be involved in the transmission of a wide variety of pathogens, but nothing is known about its associated non-pathogenic bacterial community. to address this question, we examined the composition of bacterial communities in d. gallinae collected from standard poultry farms in brittany, france. genetic fingerprints of bacterial communities were generated by temporal temperature gradient gel electrophoresis (ttge) separation of indivi ...200919027065
endosymbiotic bacteria living inside the poultry red mite (dermanyssus gallinae).this study investigated the endosymbiotic bacteria living inside the poultry red mite collected from five samples of one commercial farm from the uk and 16 farms from france using genus-specific pcr, pcr-ttge and dna sequencing. endosymbiotic bacteria are intracellular obligate organisms that can cause several phenotypic and reproductive anomalies to their host and they are found widespread living inside arthropods. the farm sampled from the uk was positive for bacteria of the genera cardinium s ...200919145467
molecular detection of rickettsia, coxiella and rickettsiella dna in three native australian tick species.three australian native animal species yielded 60 samples composed of three indigenous ticks. hosts included twelve koalas, two echidnas and one wombat from victoria, and ticks were of the species ixodes tasmani (n = 42), bothriocroton concolor (n = 8) and b. auruginans (n = 10), respectively. pcr screening and sequencing detected a species of coxiella, sharing closest sequence identity to c. burnetii (>98%), in all b. auruginans, as well as a species of rickettsia, matching closest to r. massil ...200919296229
diversity of bacteria associated with collembola - a cultivation-independent survey based on pcr-amplified 16s rrna genes.the bacterial communities found in eight different soil-inhabiting microarthropod species of the class collembola (springtails) were analyzed by analysis of pcr-amplified 16s rrna genes obtained without cultivation from total dna. to characterize the bacteria associated with the parthenogenetically-reproducing folsomia candida, the almost complete 16s rrna genes were amplified with three universal primers, i.e., the forward primer f27 and the reverse primer r1492 or r1525. with the reverse prime ...200419712416
a novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, diplorickettsia massiliensis, gen. nov., sp. nov.obligate intracellular bacteria of arthropods often exhibit a significant role in either human health or arthropod ecology.201020644718
symbiotic bacterium modifies aphid body color.color variation within populations of the pea aphid influences relative susceptibility to predators and parasites. we have discovered that infection with a facultative endosymbiont of the genus rickettsiella changes the insects' body color from red to green in natural populations. approximately 8% of pea aphids collected in western europe carried the rickettsiella infection. the infection increased amounts of blue-green polycyclic quinones, whereas it had less of an effect on yellow-red caroteno ...201021097935
genetic and electron-microscopic characterization of 'rickettsiella agriotidis', a new rickettsiella pathotype associated with wireworm, agriotes sp. (coleoptera: elateridae).wireworms, the polyphagous larvae of click beetles belonging to the genus agriotes (coleoptera: elateridae), are severe and widespread agricultural pests affecting numerous crops. a previously unknown intracellular bacterium has been identified in a diseased agriotes larva. microscopic studies revealed the subcellular structures characteristic of rickettsiella infections. molecular phylogenetic analysis based on 16s ribosomal rna and signal recognition particle receptor (ftsy) encoding sequences ...201121638041
genetic and electron-microscopic characterization of rickettsiella bacteria from the manuka beetle, pyronota setosa (coleoptera: scarabaeidae).larvae of manuka beetles, pyronota spp. (coleoptera: scarabaeidae) cause pasture damage in new zealand by feeding on the roots of grasses. surveys for potential biocontrol agents revealed a putative disease, expressed as whitened larvae of one of the outbreak species, pyronota setosa. microbial diagnosis indicated an intracoelomic, intracellular infection, and intracellular bacteria have been identified with subcellular structures characteristic of infection by rickettsiella-like microorganisms. ...201121640120
type ivb secretion systems of legionella and other gram-negative bacteria.type iv secretion systems (t4sss) play a central role in the pathogenicity of many important pathogens, including agrobacterium tumefaciens, helicobacter pylori, and legionella pneumophila. the t4sss are related to bacterial conjugation systems, and are classified into two subgroups, type iva (t4ass) and type ivb (t4bss). the t4bss, which is closely related to conjugation systems of inci plasmids, was originally found in human pathogen l. pneumophila; pathogenesis by l. pneumophila infection req ...201121743810
Metagenomic profile of the bacterial communities associated with Ixodes ricinus ticks.Assessment of the microbial diversity residing in arthropod vectors of medical importance is crucial for monitoring endemic infections, for surveillance of newly emerging zoonotic pathogens, and for unraveling the associated bacteria within its host. The tick Ixodes ricinus is recognized as the primary European vector of disease-causing bacteria in humans. Despite I. ricinus being of great public health relevance, its microbial communities remain largely unexplored to date. Here we evaluate the ...201122022422
multilocus sequence typing (mlst) for the infra-generic taxonomic classification of entomopathogenic rickettsiella bacteria.the genus rickettsiella comprises intracellular bacterial pathogens of a wide range of arthropods that are currently classified in four recognized species and numerous further pathotypes. however, both the delineation of and the synonymization of pathotypes with species are highly problematic. in the sequel of a previous phylogenomic study at the supra-generic level, nine selected genes - the 16s and 23s rrna genes and the protein-encoding genes dnag, ftsy, gida, ksga, rpob, rpsa, and sucb - wer ...201122092813
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