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inter- and intrageneric relationships of the genus propionibacterium as determined by 16s rrna sequences.the intra- and intergeneric position of propionibacterium was determined by comparing reverse transcriptase sequences of 16s ribosomal ribonucleic acid. propionibacterium jensenii. p. thoenii and p. acidipropionici formed a phylogenetically tight cluster, while p. freudenreichii, p. acnes and p. propionicus were as remotely related among each other as each of them was to the first three species. the genus propionibacterium represents a well defined taxon that stands isolated among other major gr ...19892482337
characterization of three distinct extradiol dioxygenases involved in mineralization of dibenzofuran by terrabacter sp. strain dpo360.the dibenzofuran-degrading bacterial strain dpo360 represents a new species of the genus terrabacter together with the previously described dibenzofuran-mineralizing bacterial strain dpo1361 (k.-h. engesser, v. strubel, k. christoglou, p. fischer, and h. g. rast, fems microbiol. lett. 65:205-210, 1989; v. strubel, ph.d. thesis, university of stuttgart, stuttgart, germany, 1991; v. strubel, h. g. rast, w. fietz, h.-j. knackmuss, and k.-h. engesser, fems microbiol. lett. 58:233-238, 1989). two 2,3 ...19978981980
nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column.a bacterial strain which is able to degrade pyridine was previously isolated from the oxic zone of an oil shale column and described as pimelobacter sp. strain os4t. however, pimelobacter species have been transferred to the genera nocardioides and terrabacter. strain os4t was identified as a member of the genus nocardioides on the basis of chemotaxonomic analysis and phylogenetic inference based on 16s ribosomal dna (rdna) sequence analysis. the g+c content of strain os4t is 72.5 mol%. the cell ...19979336889
terracoccus luteus gen. nov., sp. nov., an ll-diaminopimelic acid-containing coccoid actinomycete from soil.a gram-positive, aerobic actinomycete was isolated from soil. spherical cells of this organism occur singly or form packets, which may cluster. the diagnostic diamino acid of the cell wall peptidoglycan is ll-diaminopimelic acid. the predominate menaquinone is mk-8 (h4), and the main fatty acids are 13-methyl tetradecanoic acid and 12-methyl tetradecanoic acid. the diagnostic polar lipids are phosphatidylethanolamine and phosphatidylinositol. the dna base composition is 73 mol% g + c. comparison ...19979336932
inter- and intraspecific phylogenetic analysis of the genus nocardioides and related taxa based on 16s rdna sequences.the 16s rdnas from 38 strains of the genus nocardioides, two aeromicrobium species and terrabacter tumescens were directly sequenced and then analysed. the mean nucleotide similarity value between the type strains of validly described nocardioides species was 96.1 +/- 3.0%. the mean nucleotide similarity value between the type strains of validly described nocardioides species and the two aeromicrobium species was 93.7 +/- 1.4% t. tumescens was distantly related to the genera nocardioides and aer ...19989542088
microcosm enrichment of biphenyl-degrading microbial communities from soils and sediments.a microcosm enrichment approach was employed to isolate bacteria which are representative of long-term biphenyl-adapted microbial communities. growth of microorganisms was stimulated by incubating soil and sediment samples from polluted and nonpolluted sites with biphenyl crystals. after 6 months, stable population densities between 8 x 10(9) and 2 x 10(11) cfu/ml were established in the microcosms, and a large percentage of the organisms were able to grow on biphenyl-containing minimal medium p ...19989687466
genetic analyses of the genus nocardioides and related taxa based on 16s-23s rdna internally transcribed spacer sequences.the 16s-23s internally transcribed spacer (its) sequences were analysed to clarify inter- and intraspecific relationships among strains of the genus nocardioides and the relationship between two aeromicrobium species. the 16s-23s its regions from 33 nocardioides strains, two aeromicrobium species and terrabacter tumescens were sequenced directly after polymerase chain reaction (pcr0 amplification and gamma exonuclease treatment. the genomes of some nocardioides strains included two types of 16s- ...19989734018
application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria.strains belonging to the gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (tgge). this method allows the sequence-specific separation of amplified fragments of 16s rrna genes. a total of 115 reference strains representing the majority of the species of the genera aeromicrobium, agromyces, arthrobacter, aureobacterium, cellulomonas, curtobacterium, nocardioides and terrabacter were characterized. depending on the genus investigated, th ...199910028252
polyamine profiles within genera of the class actinobacteria with ll-diaminopimelic acid in the peptidoglycan.polyamine patterns of coryne- and nocardioform representatives of the class actinobacteria with ll-diaminopimelic acid in the peptidoglycan, comprising strains of the genera aeromicrobium, nocardioides, intrasporangium, terrabacter, terracoccus, propioniferax, friedmanniella, microlunatus, luteococcus and sporichthya, were analysed. the different polyamine patterns were in good agreement with the phylogenetic heterogeneity within this group of actinomycetes. strains of the closely related genera ...199910028260
isolation of terrabacter sp. strain dde-1, which metabolizes 1, 1-dichloro-2,2-bis(4-chlorophenyl)ethylene when induced with biphenyl.terrabacter sp. strain dde-1, able to metabolize 1,1-dichloro-2, 2-bis(4-chlorophenyl)ethylene (dde) in pure culture when induced with biphenyl, was enriched from a 1-1-1-trichloro-2, 2-bis(4-chlorophenyl)ethane residue-contaminated agricultural soil. gas chromatography-mass spectrometry analysis of culture extracts revealed a number of dde catabolites, including 2-(4'-chlorophenyl)-3,3-dichloropropenoic acid, 2-(4'-chlorophenyl)-2-hydroxy acetic acid, 2-(4'-chlorophenyl) acetic acid, and 4-chlo ...199910584027
'candidatus nostocoida limicola', a filamentous bacterium from activated sludge.five strains of 'candidatus nostocoida limicola' were isolated by micromanipulation from two activated sludge plants. two (ben17 and ben18) were from sunbury, victoria, australia, and three (ben67, ver1 and ver2) were from verona, italy. the near complete 16s rdna sequences were determined for five strains and the phylogenetic location of this important bulking filament in the actinomycete subphylum is reported for the first time. phylogenetically, the ben strains formed one group with 99.4% 16s ...200010758878
isolation and characterization of the genes encoding a novel oxygenase component of angular dioxygenase from the gram-positive dibenzofuran-degrader terrabacter sp. strain dbf63.a gram-positive bacterium terrabacter sp. strain dbf63 is able to degrade dibenzofuran (df) via initial dioxygenation by a novel angular dioxygenase. the dbfa1 and dbfa2 genes, which encode the large and small subunits of the dibenzofuran 4,4a-dioxygenase (dfdo), respectively, were isolated by a polymerase chain reaction-based method. dbfa1 and dbfa2 showed moderate homology to the large and small subunits of other ring-hydroxylating dioxygenases (less than 40%), respectively, and some motifs su ...200111322788
degradation of chlorinated dibenzofurans and dibenzo-p-dioxins by two types of bacteria having angular dioxygenases with different features.two kinds of bacteria having different-structured angular dioxygenases-a dibenzofuran (df)-utilizing bacterium, terrabacter sp. strain dbf63, and a carbazole (car)-utilizing bacterium, pseudomonas sp. strain ca10-were investigated for their ability to degrade some chlorinated dibenzofurans (cdfs) and chlorinated dibenzo-p-dioxins (cdds) (or, together, cdf/ds) using either wild-type strains or recombinant escherichia coli strains. first, it was shown that car 1,9a-dioxygenase (cardo) catalyzed an ...200111472938
degradation characteristics of a dibenzofuran-degrader terrabacter sp. strain dbf63 toward chlorinated dioxins in soil.to obtain basic information towards applying a dibenzofuran (df)-degrader terrabacter sp. strain dbf63 to bioremediate dioxin-contaminated soil, we investigated the degradative potential of strain dbf63 for either chlorinated or polychlorinated dibenzo-p-dioxins and dibenzofurans (clxdd/clxdf) in soil. in the soil slurry system with a soil to water ratio of 1:5 (w/v), the df-grown dbf63 cells degraded 90% of 1 ppm 2,8-cl2df, whereas only 40% of 1 ppm 2,3-cl2dd during the 7-day incubation. the de ...200212117055
plasmid-borne genes code for an angular dioxygenase involved in dibenzofuran degradation by terrabacter sp. strain yk3.the genes responsible for angular dioxygenation of dibenzofuran in actinomycetes were cloned by using a degenerate set of pcr primers designed by using conserved sequences of the dioxygenase alpha subunit genes. one sequence of alpha subunit genes was commonly amplified from four dibenzofuran-utilizing actinomycetes: terrabacter sp. strains yk1 and yk3, rhodococcus sp. strain yk2, and microbacterium sp. strain yk18. a 5.2-kb psti fragment encoding the alpha and beta subunits of the terminal diox ...200212147464
dioxin catabolic genes are dispersed on the terrabacter sp. dbf63 genome.reverse transcription-pcr of the dbfa1a2, dbfbc, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by terrabacter sp. strain dbf63. the specific expression in strain dbf63 cells grown on dibenzofuran (the model compound of dioxin; df) and/or fluorene (fn) indicated that the dbfa1a2 and dbfbc catalyze the conversion of df to salicylat ...200212163007
isolation and characterization of dibenzofuran-degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading rhodococcus species.sixteen actinomycetes capable of utilizing dibenzofuran as a sole source of carbon and energy were isolated, including rhodococcus, microbacterium, and terrabacter genera. heretofore, no dibenzofuran-utilizing strain belonging to the genus microbacterium has been reported. five extradiol dioxygenase genes (dfdb, and edil to 4) of the strain rhodococcus sp. yk2 were cloned and analyzed. the nucleotide sequence of dfdb gene was almost identical to the bphc1 gene of terrabacter sp. dpo360, which wa ...200212224629
bacterial degradation of aromatic compounds via angular dioxygenation.dioxygenation is one of the important initial reactions of the bacterial degradation of various aromatic compounds. aromatic compounds, such as biphenyl, toluene, and naphthalene, are dioxygenated at lateral positions of the aromatic ring resulting in the formation of cis-dihydrodiol. this "normal" type of dioxygenation is termed lateral dioxygenation. on the other hand, the analysis of the bacterial degradation of fluorene (fn) analogues, such as 9-fluorenone, dibenzofuran (df), carbazole (car) ...200112483604
enumeration of thermophilic bacillus species in composts and identification with a random amplification polymorphic dna (rapd) protocol.the thermophilic microbial flora of general garden and domestic wastes composts, derived from thermogenic, post-thermogenic and maturation phases, was analysed using spore and total plate counts in combination with an optimised rapd protocol. a total of 459 isolates were recovered obtained at 55 degrees c, and another 56 at 70 degrees c using tryptic soy-starch agar plates, with near-equal numbers being derived from total plate counts or spore preparations. the isolates were obtained from 11 com ...200212583723
phthalate catabolic gene cluster is linked to the angular dioxygenase gene in terrabacter sp. strain dbf63.phthalate is a metabolic intermediate of the pathway of fluorene (fn) degradation via angular dioxygenation. a gene cluster responsible for the conversion of phthalate to protocatechuate was cloned from the dibenzofuran (df)- and fn-degrading bacterium terrabacter sp. strain dbf63 and sequenced. the genes encoding seven catabolic enzymes, oxygenase large subunit of phthalate 3,4-dioxygenase (phta1), oxygenase small subunit of phthalate 3,4-dioxygenase (phta2), cis-3,4-dihydroxy-3,4-dihydrophthal ...200312658514
emended description of janibacter terrae, including ten dibenzofuran-degrading strains and janibacter brevis as its later heterotypic synonym.ten gram-positive strains that degraded dibenzofuran belonged to one homogeneous cluster with common biochemical and chemotaxonomic properties. the strains included the previously described strains [terrabacter sp.] dpo 360 and dpo 1361 and were isolated from different locations in central europe. they all shared menaquinone mk-8(h(4)) and meso-diaminopimelic acid in the cell wall and showed the same cellular fatty acid, polar lipid and riboprint patterns. chemotaxonomic properties, as well as d ...200314657136
arsenicicoccus bolidensis gen. nov., sp. nov., a novel actinomycete isolated from contaminated lake sediment.an unknown gram-positive, catalase-positive, facultatively anaerobic, non-spore-forming, coccus-shaped bacterium originating from sediment was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. chemical studies revealed the presence of a cell-wall murein based on ll-diaminopimelic acid (type ll-dpm-glycine(1)), a complex mixture of saturated, monounsaturated and iso- and anteiso-methyl-branched, non-hydroxylated, long-chain cellular fatty acids and tetrahydrog ...200415023982
employment of 16 s rdna gene sequencing techniques to identify culturable environmental eubacteria in a tertiary referral hospital.universal or 'broad-range' eubacterial polymerase chain reaction (pcr) was performed on 53 isolates from environmental water-associated sites in a haematology unit (n = 22) and the outer surfaces of cleaning lotion containers sited throughout a tertiary referral hospital (n = 31) 16 s rdna pcr was performed using two sets of universal primers, including the novel reverse primer, xb4, to generate a composite amplicon of 1068 bp, which was sequenced to obtain each isolate's identity. sequence anal ...200415142716
characterization of the upper pathway genes for fluorene metabolism in terrabacter sp. strain dbf63.genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader terrabacter sp. strain dbf63. the initial attack on both fluorene and 9-fluorenone was catalyzed by dbfa to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. the flnb protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. flnd is a heteromeric protein encoded ...200415317800
genetic characterization of the dibenzofuran-degrading actinobacteria carrying the dbfa1a2 gene homologues isolated from activated sludge.thirteen dibenzofuran (df)-utilizing bacteria carrying the df terminal dioxygenase genes homologous to those of terrabacter sp. strain dbf63 (dbfa1a2) were newly isolated from activated sludge samples. the amplified ribosomal dna restriction analysis and the hybridization analyses showed that these strains were grouped into five genetically different types of bacteria. the sequence analyses of the 16s rrna genes and the dbfa1a2 homologues from these five selected isolates revealed that the isola ...200415451113
characterization of [3fe-4s] ferredoxin dbfa3, which functions in the angular dioxygenase system of terrabacter sp. strain dbf63.dibenzofuran 4,4a-dioxygenase (dfdo) from terrabacter sp. strain dbf63 is comprised of three components, i.e., terminal oxygenase (dbfa1, dbfa2), putative [3fe-4s] ferredoxin (orf16b product), and unidentified ferredoxin reductase. we produced dbfa1 and dbfa2 using recombinant escherichia coli bl21(de3) cells as a native form and purified the complex to apparent homogeneity. we also produced and purified a putative [3fe-4s] ferredoxin encoded by orf16b, which is located 2.5 kb downstream of the ...200515717172
isolation, characterization and identification of polyhydroxyalkanoate-accumulating bacteria from activated sludge.two novel gram-positive bacteria capable of accumulating poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3hb-co-3hv)] were isolated from an anaerobic-oxic activated sludge system fed with acetate. strains lpha5 and lpha7 are motile cocci, 1-2 microm in diameter, occurring singly or in pairs. these isolates have doubling times ranging from 0.4-1.7 d and can accumulate in high levels of poly(3hb-co-3hv) (up to 44.7% of cell dry weight) when grown on complex media. furthermore, these two strains ...200016232898
the fluorene catabolic linear plasmid in terrabacter sp. strain dbf63 carries the beta-ketoadipate pathway genes, pcarhgbdcfij, also found in proteobacteria.terrabacter sp. strain dbf63 is capable of degrading fluorene (fn) to tricarboxylic acid cycle intermediates via phthalate and protocatechuate. genes were identified for the protocatechuate branch of the beta-ketoadipate pathway (pcar, pcahgbdcfij) by sequence analysis of a 70 kb dna region of the fn-catabolic linear plasmid pdbf1. rt-pcr analysis of rna from dbf63 cells grown with fn, dibenzofuran, and protocatechuate indicated that the pcahgbdcfij operon was expressed during both fn and protoc ...200516272392
terrabacter terrae sp. nov., a novel actinomycete isolated from soil in spain.a gram-positive, aerobic, long-rod-shaped, non-spore-forming bacterium (strain pplb(t)) was isolated from soil mixed with iberian pig hair. this actinomycete showed keratinase activity in vitro when chicken feathers were added to the culture medium. strain pplb(t) was oxidase-negative and catalase-positive and produced lipase and esterase lipase. this actinomycete grew at 40 degrees c on nutrient agar and in the same medium containing 5 % (w/v) nacl. growth was observed with many different carbo ...200516280515
isolation and characterization of a gene cluster for dibenzofuran degradation in a new dibenzofuran-utilizing bacterium, paenibacillus sp. strain yk5.spore-forming bacterial strains capable of utilizing dibenzofuran (df) as a sole source of carbon and energy were isolated. characteristics of the isolates justified their classification into the genus paenibacillus, and their closest relative was p. naphthalenovorans. degenerate primers for aromatic hydrocarbon dioxygenase alpha subunit (ahdoa) genes and genomic dna of the strain yk5 were used for gene isolation. the nucleotide sequences of clones of the pcr products revealed that the strain yk ...200616284749
biodegradation of dibenzofuran by janibacter terrae strain xj-1.the dibenzofuran (df)-degrading bacterium, janibacter terrae strain xj-1, was isolated from sediment from east lake in wuhan, china. this strain grows aerobically on df as the sole source of carbon and energy; it has a doubling time of 12 hours at 30 degrees c; and it almost completely degraded 100 mg/l(-1) df in 5 days, producing 2,2',3-trihydroxybiphenyl, salicylic acid, gentisic acid, and other metabolites. the dbda (df dioxygenase) gene cluster in the strain is almost identical to that on a ...200616775784
genetic diversity of dioxygenase genes in polycyclic aromatic hydrocarbon-degrading bacteria isolated from mangrove sediments.to investigate the diversity of dioxygenase genes involved in polycyclic aromatic hydrocarbon (pah)-degradation, a total of 32 bacterial strains were isolated from surface mangrove sediments, from the genera mycobacterium, sphingomonas, terrabacter, sphingopyxis, sphingobium and rhodococcus. two sets of pcr primers were constructed to detect the nida-like and nahac-like sequences of the alpha subunit of the pah ring-hydroxylating dioxygenase. pcr amplified the dna fragments from all gram-positiv ...200616923069
phycicoccus jejuensis gen. nov., sp. nov., an actinomycete isolated from seaweed.a marine actinomycete strain, designated ksw2-15(t), was isolated from a dried seaweed sample collected from a sandy beach on the coast of jeju in the republic of korea. the organism produced non-motile, non-endospore-forming, gram-positive, coccoid cells. the colonies were circular, translucent and yellow in colour with entire margins. meso-diaminopimelic acid was present as the diamino acid of the peptidoglycan. the acyl type of the muramic acid was acetyl. mycolic acids were not present. the ...200617012564
cloning and functional study of a novel aromatic-ring-hydroxylating dioxygenase gene.the aromatic-ring-hydroxylating dioxygenase is a key enzyme that initiates the biodegradation of polycyclic aromatic hydrocarbons in bacteria. in the present study, a novel dioxygenase sequence was cloned from terrabacter sp. flo using a genome walking method. the dioxygenase was cloned into pet17 and actively expressed in e.coli bl21 (de3) in co-expression with electron transfer chain proteins. the recombinant dioxygenase was found to transform phenanthrene, fluorene, pyrene and fluoranthene in ...200717644853
terrabacter aerolatus sp. nov., isolated from an air sample.a gram-positive, strictly aerobic, motile, rod- or coccoid-shaped bacterium, strain 5516j-36(t), was isolated from an air sample from jeju region, korea, and its taxonomic position was investigated by a polyphasic approach. the organism grew optimally at 30 degrees c and ph 7.0-8.0. comparative 16s rrna gene sequencing studies demonstrated that this strain was highly related phylogenetically to terrabacter terrae pplb(t) and terrabacter tumescens dsm 20308(t), showing 98.9 % sequence similarity ...200717766880
different bacterial groups for biodegradation of three- and four-ring pahs isolated from a hong kong mangrove sediment.mangrove sediments have been found to degrade three- to four-ring pahs extensively. in the present study, 11 strains from 4 genera mycobacterium (3 strains), sphingomonas (5), terrabacter (2) and rhodococcus (1) were isolated from a single surface sediment sample of a hong kong mangrove swamp, among which the terrabacter strains were isolated to grow with fluoranthene for the first time. although all four genera could degrade three- and four-ring pahs, their in situ activities in natural sedimen ...200817868985
lapillicoccus jejuensis gen. nov., sp. nov., a novel actinobacterium of the family intrasporangiaceae, isolated from stone.a novel, yellow-pigmented actinobacterium was isolated from a small stone collected in jeju, republic of korea. the cells of the organism, designated strain r-ac013(t), were gram-positive, aerobic, non-motile cocci that occurred singly or in pairs. the strain showed growth at 10-37 degrees c and ph 4.1-11.1, and tolerated 2 % nacl. on the basis of the 16s rrna gene sequence, the organism was phylogenetically related to members of the genera tetrasphaera (94.6-97.1 % sequence similarity), terraba ...200718048726
dde remediation and degradation.ddt and its metabolites, ddd and dde, have been shown to be recalcitrant to degradation. the parent compound, ddt, was used extensively worldwide starting in 1939 and was banned in the united states in 1973. the daughter compound, dde, may result from aerobic degradation, abiotic dehydrochlorination, or photochemical decomposition. dde has also occurred as a contaminant in commercial-grade ddt. the p,p'-dde isomer is more biologically active than the o,p-dde, with a reported half-life of -5.7 ye ...200818069646
characterization of a novel angular dioxygenase from fluorene-degrading sphingomonas sp. strain lb126.in this study, the genes involved in the initial attack on fluorene by sphingomonas sp. strain lb126 were investigated. the alpha and beta subunits of a dioxygenase complex (flna1-flna2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran degrader terrabacter sp. strain dbf63, were identified. when overexpressed in escherichia coli, flna1-flna2 was responsible for the angular oxidation of fluorene, 9-hydroxyfluorene, ...200818156320
sanguibacter soli sp. nov., isolated from soil of a ginseng field.a gram-positive, non-spore-forming, rod-shaped, motile bacterium, designated strain dcy22(t), was isolated from soil of a ginseng field in south korea and characterized in order to determine its taxonomic position. 16s rrna gene sequence analysis revealed that strain dcy22(t) belonged within the family sanguibacteraceae, and highest levels of sequence similarity were found with sanguibacter marinus 1-19(t) (96.8 %), sanguibacter suarezii st-26(t) (96.0 %), sanguibacter inulinus st-50(t) (95.9 %) ...200818319451
real-time pcr quantification of pah-ring hydroxylating dioxygenase (pah-rhdalpha) genes from gram positive and gram negative bacteria in soil and sediment samples.real-time pcr based assays were developed to quantify gram positive (gp) and gram negative (gn) bacterial populations that are capable of degrading the polycyclic aromatic hydrocarbons (pah) in soil and sediment samples with contrasting contamination levels. these specific and sensitive real-time pcr assays were based on the quantification of the copy number of the gene that encodes the alpha subunit of the pah-ring hydroxylating dioxygenases (pah-rhdalpha), involved in the initial step of the a ...200818329116
two angular dioxygenases contribute to the metabolic versatility of dibenzofuran-degrading rhodococcus sp. strain ha01.rhodococcus sp. strain ha01, isolated through its ability to utilize dibenzofuran (dbf) as the sole carbon and energy source, was also capable, albeit with low activity, of transforming dibenzo-p-dioxin (dd). this strain could also transform 3-chlorodibenzofuran (3cdbf), mainly by angular oxygenation at the ether bond-carrying carbon (the angular position) and an adjacent carbon atom, to 4-chlorosalicylate as the end product. similarly, 2-chlorodibenzofuran (2cdbf) was transformed to 5-chlorosal ...200818441103
terrabacter lapilli sp. nov., an actinomycete isolated from stone.a novel actinomycete, designated strain lr-26t, was isolated from a small stone collected from an agricultural field in jeju, republic of korea. cells of the organism were strictly aerobic, gram-positive, non-motile, short rods. colonies were bright yellow, circular, smooth and translucent. the organism was characterized chemotaxonomically as having ll-diaminopimelic acid in the cell wall, mk-8(h4) as major menaquinone, a polar lipid profile including diphosphatidylglycerol, phosphatidylethanola ...200818450693
the gaf-like-domain-containing transcriptional regulator dfdr is a sensor protein for dibenzofuran and several hydrophobic aromatic compounds.dibenzofuran (df) is one of the dioxin carbon skeletal compounds used as a model to study the microbial degradation of dioxins. this study analyzed the transcriptional regulation of the df dioxygenase genes dfda1 to dfda4 in the df-utilizing actinomycetes rhodococcus sp. strain yk2 and terrabacter sp. strain yk3. an open reading frame designated dfdr was detected downstream of the dfdc genes. the c-terminal part of the dfdr amino acid sequence has high levels of similarity to several luxr-type d ...200918952799
fodinibacter luteus gen. nov., sp. nov., an actinobacterium isolated from a salt mine.a gram-positive-staining, aerobic, catalase- and oxidase-positive, irregular short rod-shaped actinobacterium, designated strain yim c003t, was isolated from a salt mine in yunnan, pr china. phylogenetic analysis based on 16s rrna gene sequences showed that strain yim c003t was most closely related to strains of the genera knoellia (94.2-96.0% similarity), oryzihumus (95.6%), terrabacter (94.9-95.4%), janibacter (94.9-95.4%), kribbia (95.0%), lapillicoccus (95.0%) and phycicoccus (94.2-95.0%) of ...200919605729
terrabacter terrigena sp. nov., isolated from soil.a gram-positive-staining, non-motile and rod-shaped bacterium, strain on10(t), was isolated from soil around a wastewater treatment plant in korea and its taxonomic position was investigated by using a polyphasic approach. strain on10(t) grew optimally at ph 6.5-7.0 and 30 degrees c in the presence of 0.5 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain on10(t) clustered with the clade comprising terrabacter species, with which it exhibited 16s rrna gene se ...200919628617
terrabacter aeriphilus sp. nov., isolated from an air sample.a novel actinomycete, designated strain 5414t-18(t), was isolated from an air sample collected from the taean region, korea. the strain contained oxidase and grew in the presence of 7 % nacl. a neighbour-joining tree constructed on the basis of the 16s rrna gene sequence showed that strain 5414t-18(t) is a member of the genus terrabacter, sharing 97.8-98.3 % 16s rrna gene sequence similarities to type strains of species of the genus terrabacter (98.3 % sequence similarity with terrabacter lapill ...201019666794
aromatic hydrocarbon degradation genes from chronically polluted subantarctic marine sediments.the goal of this study was to identify functional targets to detect polycyclic aromatic hydrocarbon (pah)-degrading bacterial populations in cold marine ecosystems.200919780966
[bacterial diversity recovered from qiyi glacier and runoff, qilian mts].abundance and species diversity of bacteria were investigated respectively by epifluorescence microscope, the culture method and the analysis of 16s rdna genes, with snow and runoff samples from qiyi glacier in the qilian mts. according to the results, the total microbial cells and bacterial cfu range from 10(3) to 10(5) cells x ml(-1) and 0-600 cfu x ml(-1), respectively. the 16s rdna gene of 22 bacterial isolates recovered from snow and runoff samples belong to following groups: bacteroidetes, ...200919927833
terrabacter carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete.a bacterial strain, py2(t), capable of oxidizing carbon monoxide, was isolated from a soil sample collected from a roadside at yonsei university, seoul, korea. on the basis of 16s rrna gene sequence analysis, strain py2(t) was shown to belong to the genus terrabacter and was most closely related to terrabacter lapilli lr-26(t) (99.1 % similarity). strain py2(t) was characterized chemotaxonomically as having iso-c(15 : 0) as the predominant fatty acid, mk-8(h(4)) as the major menaquinone, ll-diam ...201120207805
identification and characterization of a novel terrabacter ginsenosidimutans sp. nov. beta-glucosidase that transforms ginsenoside rb1 into the rare gypenosides xvii and lxxv.a new beta-glucosidase from a novel strain of terrabacter ginsenosidimutans (gsoil 3082(t)) obtained from the soil of a ginseng farm was characterized, and the gene, bgpa (1,947 bp), was cloned in escherichia coli. the enzyme catalyzed the conversion of ginsenoside rb1 {3-o-[beta-d-glucopyranosyl-(1-2)-beta-d-glucopyranosyl]-20-o-[beta-d-glucopyranosyl-(1-6)-beta-d-glucopyranosyl]-20(s)-protopanaxadiol} to the more pharmacologically active rare ginsenosides gypenoside xvii {3-o-beta-d-glucopyran ...201020622122
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