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[study of the possibility of utilizing the transpired mositure condensate from sweet potato for growing plants in biological life support systems].the effect of nonpurified condensate obtained during prolonged cultivation of batata in a sealed chamber upon batata cuttings and seedlings of garden cress, radish and chinese cabbage was studied. it was shown that nonpurified condensate produced an inhibitory effect on the formation of roots in batata cuttings and on the growth of previously developed roots of batata cuttings and seedlings. the studies which used a chemical model of 3,4-dihydroxy phenylalanine indicated that the condensate cont ...200913246
herbicidins c and e, two new necleoside antibiotics.streptomyces saganonensis no. 4075 used for production of herbicidins a and b has also produced other two antibiotics, herbicidins c and e in submerged culture. isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous meoh and completed by silica-gel chromatography. physico-chemical characteriation revealed that herbicidins c and e are new nucleoside antibiotics having an adenine moiety in their structures. herbicidins c and e indicated inhi ...1979117003
[equation of the helix for separating plants in a spiral-radial cultivator].highly effective and compact cultivators must pullapart plants during vegetation in order to save light energy and area under crops. cultivators ensuring a helical method for pulling plants apart are already available. the present paper suggests the criteria of the efficiency of using the area under crops of the cultivator and deformation of the leaf crown of plants (sigma- and delta-spectra). with respect to these values the main task of calculating the helix has been formulated. having chinese ...2012234550
[the effect of actinomycin d on the production of viruses by the protoplasts of chinese cabbage infected in vitro by the yellow mosaic virus of turnips].chinese cabbage (brassica sinensis l. var. cantonner) protoplasts were infected by turnip yellow mosaic virus (tymv) and inoculated in the presence or absence of actinomycin d. virus production was determined 40 hrs. after inoculation, the time required for the virus replication cycle to be terminated. while actinomycin d had no effect on tymv production when present at a concentration of 1 microng/ml, a 50 to 80% inhibition of virus production was noticed at concentrations of the order of 5 to ...1977405160
populations of the spinach wilt pathogen, fusarium oxysporum f. sp. spinaciae, in the root tissues, rhizosphere, and soil in the field.populations of fusarium oxysporum f. sp. spinaciae in root tissues and rhizosphere soil of diseased spinach plants were higher than in the root tissues and rhizosphere soil of healthy plants. populations in soil rhizosphere were higher than in nonrhizosphere soil. the fungus populations were very low in the root tissues of the nonsusceptible strawberry, broccoli, chinese cabbage, and mustard grown in the infested field. the populations were low at the beginning of the season, increased, and rema ...1979436018
synthesis and accumulation of polyamines and s-adenosylmethionine in chinese cabbage infected by turnip yellow mosaic virus. 1979465028
effect on growth and nickel content of cabbage plants watered with nickel solutions.chinese cabbage plants were watered with different concentrations of nicl2 solutions and the effect on growth and uptake of nickel in the plants were studied. no toxic effect on plant growth was observed. a higher content of nickel was found in the plants exposed to more concentrated nickel solutions. nickel contamination and its clinical consequences are discussed.1979498768
structure of plant nuclear and ribosomal dna containing chromatin.digestion of plant chromatin from brassica pekinensis and matthiola incana with staphylococcus nuclease leads to a dna repeat of 175 plus or minus 8 and a core size of 140 base pairs. dnase i digestion results in multiples of 10 bases. ribosomal rnn genes were studied as a model system for active plant chromatin because of their great redundancy and their high transcriptional activity in growing and differentiating tissues. the actively transcribed genes were identified by nascent rna of ribosom ...1979514813
glucosinolates and derived products in cruciferous vegetables. analysis of 14 varieties of chinese cabbage. 2000762327
[in vitro infection of chinese cabbage protoplasts by the turnip yellow mosaic virus]. 1975808296
isolation of viruses by electro-extraction of infected plants.the isolation of viruses from infected plant material by a process termed electro-extraction appeared to be a convenient and simple method of obtaining viruses in a fair state of purity. the method has the advantage over the conventional methods of virus purification that the infected plant tissue is not disintegrated and that organic solvents such as chloroform and butanol are avoided. the procedure used was demonstrated on the extraction of tobacco mosaic virus (tmv) from infected tobacco and ...1977909872
inhibition of turnip yellow mosaic virus synthesis by pyrimidine analogues.the pyrimidine analogues 2-thiouracil, 2-thiouridine, 6-azauracil and 6-azauridine all inhibited the synthesis of turnip yellow mosaic virus (tymv) and increased the synthesis of empty virus protein shells in infected chinese cabbage leaf discs. uracil and uridine reversed these effects. 2-thiouracil also reduced the utp pool in tymv infected leaf discs. the results are consistent with the suggestion that these analogues or their in vivo derivatives affect virus synthesis by inhibiting the biosy ...1976989025
herbicidins a and b, two new antibiotics with herbicidal activity. i. producing organism and biological activities.herbicidins a and b, two new antibiotics with selective and contact herbicidal activity, were produced by a new species of streptomyces designated as s. sagononensis. among tested microorganisms, herbicidins indicated some activity against fungi in vitro and xanthomonas oryzae in vivo. their characteristic features were brought into relief by their selective and contact killing effect on many dicotyledonous plant as well as their inhibition of germination of the plant seeds, such as chinese cabb ...1976993127
a chlorophyll-protein complex lacking in photosystem i mutants of chlamydomonas reinhardtii.sodium dodecyl sulfate gel electrophoresis of unheated, detergent-solubilized thylakoid membranes of chlamydomonas reinhardtii gives two chlorophyll-protein complexes. chlorophyll-protein complex i (cp i) is the blue-green in color and can be dissociated by heat into "free" chlorophyll and a constituent polypeptide (polypeptide 2; mol wt 66,000). similar experiments with spinach and chinese cabbage show that the higher plant cp i contains an equivalent polypeptide but of slightly lower molecular ...19751194353
effects of kinetin on phosphorylation of leaf membrane proteins.isolated chinese cabbage leaf membranes were phosphorylated by membrane-associated protein kinase(s) in the presence or [gamma-32p]atp. membrane-associated 32p radioactivity appeared to be bound to membrane proteins. both smooth cell membranes and chloroplast lamellae reacted with atp. phosphorylation of the membranes was inhibited by ca2+ and partially inhibited by kinetin or 6-benzyladenine. the possibility that cytokinin effects on membrane phosphorylation might increase ion availability was ...19761252471
location, rate and asymmetry of ds-rna synthesis during replication of tymv in chinese cabbage.the ds-rna induced by tymv infection in the nuclei of infected chinese cabbage leaf cells became labelled with [32p]phosphate most rapidly before production of virus could be detected. in contrast, the ds-rna associated with the chloroplasts became labelled most rapidly during the period of maximum virus production.19761275406
proceedings: replication of tymv in chinese cabbage protoplasts. 19761275407
proceedings: biochemical studies on rna synthesis in healthy and tymv infected chinese cabbage: effect of actinomycin d. 19761275408
proceedings: autoradiographical studies on rna synthesis in healthy and tymv infected chinese cabbage: effect of actinomycin d. 19761275409
localization of cauliflower mosaic virus in the cell nucleus of brassica pekinensis l.cauliflower mosaic virus (camv) particles were observed in the nuclei of xylem parenchyma cells in brassica pekinensis l. doubly infected by camv and turnip mosaic virus (tumv). camv particles were aggregated in the nucleoplasm but not embedded in viroplasms. this phenomenon was not detected in cell nuclei of mesophyll tissue. typical features associated with infection by either camv or tumv normally occurred in the cytoplasm of cells of both tissues: two types of viroplasms with embedded camv p ...19921410828
turnip yellow mosaic virus variants produced from dna clones encoding their genomes.full-length dsdna clones that encode the genomes of two australian turnip yellow mosaic isolates, tymv-bl and tymv-cl have been constructed. these clones were transcribed to give 6.3 kb capped ssrna which infects chinese cabbages to give symptoms indistinguishable from those produced by the parental viruses. extensions of up to 26 nucleotides at the 3' end of the tymv-bl clone delay infections, but virus particles isolated from these plants 4 weeks after inoculation contain rna with the original ...19921456892
second-site suppressor mutations assist in studying the function of the 3' noncoding region of turnip yellow mosaic virus rna.the 3' noncoding region of turnip yellow mosaic virus rna includes an 82-nucleotide-long trna-like structure domain and a short upstream region that includes a potential pseudoknot overlapping the coat protein termination codon. genomic rnas with point mutations in the 3' noncoding region that result in poor replication in protoplasts and no systemic symptoms in planta were inoculated onto chinese cabbage plants in an effort to obtain second-site suppressor mutations. putative second-site suppre ...19921501271
[nucleic acid components in carbohydrate-rich food].the content of nucleic acid components in numerous foods, especially carbohydrate-rich ones, has been investigated. the data obtained for bases (purines and pyrimidines) were calculated as nucleic-acid equivalents (rna or dna); the imp content was calculated from the measured content of hypoxanthine. not only did cultivated plants such as cereals and pulses show a high rna-equivalent content but also vegetables such as spinach, leek, broccoli, chinese cabbage and cauliflower. we found the same r ...19901689090
expression of orf-69 of turnip yellow mosaic virus is necessary for viral spread in plants.turnip yellow mosaic virus (tymv) rna has two extensively overlapping open reading frames (orfs) encoding noncapsid proteins. the longest of these, orf-206, is essential for rna replication. we have investigated the expression and role of orf-69 (encoding a protein with a mw of 69k) using specific antiserum and mutant genomic rnas designed to interrupt orf-69 expression. tyc69 antiserum immunoprecipitated a protein with an apparent mw of 75k (p69) from in vitro translations of tymv rna. mutant r ...19921736523
turnip yellow mosaic virus rnas with anticodon loop substitutions that result in decreased valylation fail to replicate efficiently.single and multiple nucleotide substitutions have been introduced into the anticodon loop of the trna-like structure of turnip yellow mosaic virus (tymv) genomic rna. we studied the effects of these mutations on in vitro valylation and on replication in chinese cabbage protoplasts and plants. only those mutants capable of efficient and complete valylation showed efficient replication in protoplasts and gave rise to systemic symptoms in whole plants. mutants that accepted valine inefficiently (in ...19912033666
first isolation of xanthomonas campestris from the blood of a chinese woman.xanthomonas campestris isolated from the blood of a patient with a fever was first reported. xanthomonas campestris is a bacterium that can cause black rot of some vegetables, such as rape. chinese cabbage, etc. human infection due to x. campestris has not been reported so far. the characteristics of this organism, including morphology, staining, physiology and biochemistry were studied. we believe that x. campestris is also one of the opportunistic pathogens, which can infect compromised host.19902118062
induction of nitroarenes in cigarette smoke condensate treated with nitrate.samples of cigarette smoke condensates (cscs) treated with nitric acid or chinese cabbage pickles having a high nitrate content strongly mutated salmonella typhimurium strain ta98; the benzene/ethanol extract after these treatments induced 1800 and 820 revertants, respectively, per mg of extract for strain ta98 in the absence of s9 mix. the major mutagens in these materials were found to be 1-nitropyrene and 1,3-dinitropyrene on the basis of the result of gas chromatography/mass spectrometry and ...19902155483
[antimutagenic activity of vegetable and fruit extracts against in-vitro benzo(a)pyrene].about 80% of the juices from twenty vegetables and fruits showed antimutagenic activity when tested in the presence of the mutagen and carcinogen benzo[a]pyrene using the ames' salmonella/microsome assay. in a standardized test system juices from raw celeriac, broccoli, red cabbage, carrots, green, peppers, lettuce, asparagus, apricots, red-currants, gooseberries, raspberries, and pineapple showed more than 50% inhibition. leek, kohlrabi, cucumber, zucchini, french beans, fennel leaves, rhubarb, ...19902336853
infectious tymv rna from cloned cdna: effects in vitro and in vivo of point substitutions in the initiation codons of two extensively overlapping orfs.full-length cdna of the 6.3 kb turnip yellow mosaic virus (tymv) genome was placed between a t7 promoter and a unique hind iii site. in vitro transcription of hind iii-linearized dna of clone ptymc yielded full-length rna transcripts. in inoculations of chinese cabbage protoplasts and plants, capped transcripts and virion rna had similar specific infectivities and produced similar systemic symptoms. we have used the ptymc clone in studies of the expression of two overlapping open reading frames ...19892473440
[preliminary observation on feeding preference of oncomelania for sedum sarmentosum].as a result of screening, we discovered that sedum sarmentosum bunge was most preferred among 317 kinds of snail food. both adult and young snails readily eat s. sarmentosum as evidenced by laboratory observation and dissection of the snails. in aquarium, more snails were attracted by s. sarmentosum than by chinese cabbage and chlorella suspension. on water surface, s. sarmentosum curtains attracted adult snails 2.1-3.7 times and immature snails 2.3-4.1 times more than rice straw curtains.19892591039
[multiple stepwise regression analysis of etiological factors of esophageal cancer in cixian county].cixian county, one of the high-risk counties of esophageal cancer in the world, has a standardized mortality of 142.19/10(5) population, 1969-1971. the incidence of esophageal cancer had dropped year by year from 1974 to 1982. the significance of the incidence tendency was studied. the results are highly significant (p less than 0.001). the causative factors of esophageal cancer including five independent variables: x1 (number of people taking sanitized water), x2 (number of people on pickled ch ...19892789130
immunochemical properties of elongation factors 1 of plant origin.elongation factors 1 (ef-1) have been isolated from different plants: wheat, yellow lupine, blue lupine, chinese cabbage and norway maple. antibodies for ef-1 from yellow lupine have been obtained in rabbits; antibodies for wheat ef-1 were elicited in mice. the immunological properties of ef-1 were assayed by the following methods: western blotting, double immunodiffusion and rocket immunoelectrophoresis. our results suggest that one antigenic site is similar for all plant elongation binding fac ...19883123226
residues of dacthal and metabolites in field-treated chinese cabbage. 19883233362
4-methoxyindole derivatives as nitrosable precursors of mutagens in chinese cabbage.two indole compounds isolated from fresh chinese cabbage were shown to be mutagen precursors that yielded direct-acting mutagens on treatment with nitrite. they were identified as 4-methoxyindole-3-acetonitrile and 4-methoxy-indole-3-aldehyde. when these compounds were treated with 50 mm nitrite at ph 3.0 for 1 h at 37 degrees c, 4-methoxyindole-3-acetonitrile induced 31,800 and 10,000 revertants of salmonella typhimurium ta100 and ta98, respectively, per mg of mutagen precursor in the absence o ...19863331681
diet and cancer of the stomach: a case-control study in china.a case-control study focusing on the role of single food items was undertaken in the heilongjiang area, 241 patients with histologically verified stomach cancer and an equal number of controls (with non-neoplastic diseases) matched by age, sex and area of residence being interviewed during a 2-year period. questions asked covered economic status, occupation, histories of smoking and drinking, diet and dietary habits and disease history. data concerning the average frequency and quantity of consu ...19883346096
recently identified nitrite-reactive compounds in food: occurrence and biological properties of the nitrosated products.various japanese foodstuffs are directly-acting mutagens in salmonella typhimurium ta100 after nitrite treatment. such mutagen precursors include tyramine and beta-carboline derivatives, isolated from soya sauce, and indole-3-acetonitrile, 4-methoxyindole-3-acetonitrile and 4-methoxyindole-3-aldehyde, isolated from fresh chinese cabbage. a mutagen produced from tyramine with nitrite was found to be 4-(2-aminoethyl)-6-diazo-2,4-cyclohexadienone (3-diazotyramine), and one produced from indole-3-ac ...19873679387
a mutagen precursor in chinese cabbage, indole-3-acetonitrile, which becomes mutagenic on nitrite treatment.after treatment with nitrite, chinese cabbage showed direct-acting mutagenicity on salmonella typhimurium ta100 inducing 3100 revertants per g. one of the mutagen precursors that became mutagenic after nitrite treatment was isolated, and identified as indole-3-acetonitrile. after treatment with nitrite, 1 mg of indole-3-acetonitrile induced 17 400 revertants of ta100 and 21 000 revertants of ta98 without s9 mix.19853889600
nitrosatable precursors of mutagens in vegetables and soy sauce.nitrosatable precursors of mutagens that show mutagenicity to salmonella typhimurium ta100 without s9 mix after treatment with nitrite at ph 3 were found in various foods. from chinese cabbage, three indole compounds, indole-3-acetonitrile, 4-methoxyindole-3-acetonitrile, and 4-methoxyindole-3-aldehyde, were identified as mutagen precursors. 1-methylindole and 2-methylindole, which are present in cigarette smoke showed strong mutagen precursor activity. escherichia coli wp2 uvra/pkm101 is more s ...19853916206
mutagenic compounds in soy sauce, chinese cabbage, coffee and herbal teas. 19863952098
the incorporation of radiolabeled polyamines and methionine into turnip yellow mosaic virus in protoplasts from infected plants.turnip yellow mosaic virus contains large amounts of nonexchangeable spermidine and induces an accumulation of spermidine in infected chinese cabbage. by 7 days after inoculation, a majority of protoplasts isolated from newly emerging leaves stain with fluorescent antibody to the virus. these protoplasts contain 1-2 x 10(6) virions per cell and continue to produce virus in culture for at least 48 hr. [14c]spermidine (10 microm) was taken up by these cells in amounts comparable to the original en ...19854060587
the effects of dicyclohexylamine on polyamine biosynthesis and incorporation into turnip yellow mosaic virus in chinese cabbage protoplasts infected in vitro.we have reported (r. balint and s. s. cohen, 1985, virology 144, 181-193) that protoplasts from plants infected with turnip yellow mosaic virus (tymv) continue to produce virus in culture and that newly formed virus particles contained predominantly newly synthesized spermidine and spermine. inhibition of spermidine synthesis by dicyclohexylamine (dcha), however, led to incorporation of preexisting spermidine and increased amounts of spermine into newly formed virions. we now report similar resu ...19854060588
permethrin and its two metabolite residues in seven agricultural crops.metabolite residues of permethrin are not reported in the literature for most agricultural crops. this paper reports residues of permethrin and its 2 metabolites (dichlorovinyl acid and metaphenoxybenzyl alcohol) in 7 different agricultural crops (chinese cabbage, spinach, asparagus, raspberries, green peas, turnip roots, and turnip greens). permethrin residues declined approximately 85% within 7 days after treatment in all crops. in most cases, the acid metabolite residues peaked at 3 days, and ...20134086440
some ultrastructural and cytochemical observations on chinese cabbage leaves infected with cauliflower mosaic virus. 19724111055
cell wall protrusions induced by cauliflower mosaic virus in chinese cabbage leaves: a cytochemical and autoradiographic study. 20144135909
the binding of kinetin to plant ribosomes.the synthetic cytokinins kinetin and 6-benzylaminopurine exhibit equilibrium-type binding to purified chinese-cabbage leaf ribosomes. at 23mum and 4 degrees c one molecule of kinetin and 1.34 molecules of 6-benzylaminopurine are bound per ribosome. adenine and adenine derivatives that are inactive as cytokinins showed much less affinity for ribosomes. pretreatment of ribosomes with 0.5m-ammonium chloride or triton x-100 did not decrease the extent of cytokinin binding. binding appeared to be to ...20154320923
evidence for modification of protein phosphorylation by cytokinins.kinetin stimulated phosphorylation of protein in floated chinese-cabbage leaf discs, but inhibited protein phosphorylation in nuclei+chloroplast extracts from chinese-cabbage or tobacco leaves. kinetin also inhibited protein phosphorylation in isolated tobacco nuclei or nuclei from carrot secondary-phloem tissue. purified chinese-cabbage leaf ribosomes exhibited protein kinase activity which was inhibited by kinetin and zeatin. the ribosome-associated kinase responded to kinetin and zeatin diffe ...19724348261
fine structure of vesicles induced in chloroplasts of chinese cabbage leaves by infection with turnip yellow mosaic virus. 19734584930
leaf ontogeny and virus replication in brassica pekinensis infected with turnip yellow mosaic virus. 19724623503
evidence for tymv-induced rna and dna synthesis in the nuclear fraction from infected chinese cabbage leaves. 19744820885
the sequence of early cytological changes in chinese cabbage leaf cells following systemic infection with turnip yellow mosaic virus. 19744833533
studies on environmental contamination by uranium. 4. uranium absorption by chinese cabbage. 19744842199
crystallization of turnip yellow mosaic virus in wilted leaves of chinese cabbage by treatment at high temperature. 19715315697
rna-dependent rna polymerase in chinese cabbage. 19715572618
properties of ribulose-1,5-diphosphate carboxylase (carboxydismutase) from chinese cabbage and photosynthetic microorganisms.ribulose-1,5-diphosphate carboxylase (carboxydismutase) was prepared from chinese cabbage [brassica petsai (parl)] and the k(m) values and molecular weight were determined. these parameters were found to be in good agreement with values reported for this enzyme from other higher plants. investigation of carboxydismutase activity from the photosynthetic micro-organisms chlamydomonas reinhardi (iu 89+), plectonema boryanum (iu 594), and chromatium strain d showed striking similarity to the higher ...19685700019
ribonuclease isozymes in chinese cabbage systemically infected with turnip yellow mosaic virus. 19685723669
some effects of kinetin on floated chinese cabbage leaf discs. 19695792855
virus infection and photosynthesis. 1. increased photophosphorylation by chloroplasts from chinese cabbage infected with turnip yellow mosaic virus. 19655850878
electron microscopic and biochemical characterization of fraction i protein.electron micrographbs of high resolution have been obtained from fraction i protein of chinese cabbage leaves. the protein, which has ribulose-1,5-diphosphate carboxylase activity, appear to be a cube swth edge of about 120 angstroms. substrtucture canl be seen in individual particles, conlsistent with a model having 24 subunits, the number prescribed by the available physical and chlemical data.19655866657
cytological changes induced by turnip yellow mosaic virus in chinese cabbage leaves. 19665938885
length requirements for trna-specific enzymes and cleavage specificity at the 3' end of turnip yellow mosaic virus rna.this paper describes the minimum length of the turnip yellow mosaic virus (tymv) rna necessary to fulfill the trna-like properties of the viral rna: 50 to 75 nucleotides and 86 nucleotides from the 3' end of tymv rna are sufficient for adenylation and valylation respectively by the escherichia coli system. the size of the trna-like fragments obtained in vitro in the presence of an e. coli, a reticulocyte or a chinese cabbage leaf extract has also been determined. among the major fragments libera ...19826176943
detection of partially proteolysed cauliflower mosaic virus coat protein in infected leaf tissue by western blotting.cauliflower mosaic virus (camv) capsid polypeptides were detected by immunoelectroblotting ('western blotting') 10-16 days after infection of chinese cabbage leaves. the predominant polypeptides detected had molecular weights of 42,000 and 37,000 suggesting that in vivo proteolysis of the 55,000-58,000 molecular weight coat protein had taken place. the use of laboratory-made nitrocellulose membranes for western blotting is reported. phosphate-sds buffer was more suitable than tris-glycine buffer ...19836352722
determination of residues of fensulfothion and its metabolites in chinese cabbage, japanese radish , and turnips.a method was developed using a selective nitrogen-phosphorus detector to determine the combined total residues, as the oxygen analogs, of fensulfothion (o,o-diethyl o-[p-(methylsulfinyl)-phenyl] phosphorothioate) and its 3 metabolites. the procedure was used to determine harvest residues in chinese cabbage, japanese radish, and turnips treated with 2 application rates of fensulfothion. residues found after application of 2 oz active ingredient (ai)/100 lineal ft of row ranged from 0.026 ppm for ...19806447687
analysis and occurrence of total n-nitroso compounds in the japanese diet.a quantitative technique for differentiating the total n-nitrosamides from the total n-nitroso compounds has been devised. the principle of this method is based on the chemiluminescence response of a nitroso compound under different denitrosating conditions. we also examined the effect of nitrite scavengers on the stability of n-nitroso compounds and found that both ammonium sulfamate and hydrazine sulfate can be satisfactorily used for the detection of chemiluminescence response with hydrogen b ...19846533016
[hydroxycinnamic acid esters in brassicaceous vegetables and garden cress].the esters of caffeic, p-coumaric and ferulic acid with glucose or quinic acid and sinapoylglucose were determined in cauliflower, kale, brussel sprouts, red, white, savoy and chinese cabbage and in garden cress by two different hplc methods with gradient elution. in addition the occurrence of sinapin (sinapoylcholin) was checked. the pattern of hydroxycinamic acid esters in the brassica species varied appreciably. in garden cress besides sinapin only sinapoylglucose was found. sinapoylglucose i ...19836613360
transfer factors of some selected radionuclides (radioactive cs, sr, mn, co and zn) from soil to leaf vegetables.transfer factors of radionuclides from soil to leaf vegetables (cabbage, chinese cabbage, komatsuna, spinach and lettuce) have been studied by radiotracer experiments using andosol as a representative of japanese soils. the transfer factors of radioactive cs, sr, mn, co and zn for edible parts of vegetables (average of five vegetables) were 0.11, 0.24, 0.61, 0.05 and 0.52, respectively. these values should be used in safety assessment for japanese agricultural environment. the transfer factors o ...19957473347
nucleotide sequence of cdna clone encoding a metallothionein-like protein from chinese cabbage. 19957610190
nucleotide sequence of a cdna clone encoding the complete precursor for the "10-kilodalton" polypeptide of photosystem ii from chinese cabbage. 19957610192
preliminary x-ray diffraction analysis of crystals of turnip yellow mosaic virus (tymv).turnip yellow mosaic virus (tymv) was purified from chinese cabbage and crystallized in a form that permits high resolution structural analysis using x-ray diffraction. the crystals have a hexagonal bipyramidal morphology and often achieve dimensions of 1.0 x 1.0 x 0.5 mm. the crystals appear to be of hexagonal space group p6(2)22 with a=b=525 a, c=315 a, but we cannot strictly rule out the possibility that the space group is p622. they appear different than any crystals of tymv previously repor ...19957716173
lead and cadmium in some berries and vegetables on the finnish market in 1991-1993.in order to determine the present contribution of vegetables and berries to the intake of lead and cadmium in finland major vegetable groups as well as strawberries and blackcurrants were collected from wholesale distribution centres, orchards and the food industry. pooled samples were mixed to represent wholesale distribution centres, exporting countries or geographical regions. lead and cadmium were determined after wet digestion in concentrated hno3 using etaas with zeeman effect background c ...20137781823
evaluation of brassicas in grazing systems for sheep: i. quality of forage and animal performance.four years of grazing trials were conducted with brassica forages to evaluate their chemical composition and effect on adg of fattening lambs and breeding ewes in late fall. brassicas tested included kales (brassica oleracea l. var. acephala dc), turnips (b. rapa l.), and a chinese cabbage hybrid (b. rapa l. x b. pekinensis [lour.] rupr.). daily gains of lambs varied widely among years (19 to 330 g/d); adg on brassicas were, however, generally higher than on stockpiled kentucky 31 tall fescue (f ...19947928762
evaluation of brassicas in grazing systems for sheep: ii. blood composition and nutrient status.blood composition of lambs grazing brassicas and stockpiled grass or grass-clover pastures in the fall of 4 yr was monitored to assess possible effects of plant metabolites (e.g., glucosinolates, s-methyl cysteine sulfoxide) on health and performance. serum thyroxine (t4) concentrations in lambs grazing brassicas decreased upon initiation of grazing, with a subsequent recovery, and concentrations were increased by oral dosing with i or i+cuo. serum triiodothyronine (t3) increased gradually with ...19947928763
fermentation and sensory characteristics of kimchi containing potassium chloride as a partial replacement for sodium chloride.a study was designed to determine the effects of substituting up to 50% of the nacl in kimchi, a fermented chinese cabbage (brassica pekinensis) product containing scallions, garlic, ginger and hot red pepper powder, with kcl. brine water (15% salt) used to soak cabbage contained nacl:kcl ratios of 1:0 (control), 5:1, 2:1 and 1:1 (wt:wt). total acidity and ph of kimchi reached acceptable ranges of 0.4-0.6% (as lactic acid) and 4.4-4.7, respectively, after 13 days of incubation at 13 +/- 1 degree ...19948043352
feed intake and apparent digestibility of hay-supplemented brassica diets for lambs.animal performance on brassica diets often does not reflect laboratory estimates of the nutritive value of the herbage. hay supplementation of brassica pastures should increase diet dm and fiber intake and dilute anti-quality factors. the optimal hay:brassica ratio needed to achieve desirable animal performance, however, has not been established. effects of changing the hay:brassica ratio on feed and water intake, apparent digestibility of diets, thyroid status, and anemia were examined in five ...19948071189
structure-activity relationships of brassinin in preventing the development of carcinogen-induced mammary lesions in organ culture.brassinin, a phytoalexin, is found in chinese cabbage. previously, we showed that brassinin significantly inhibited dimethylbenz(a)anthracene (dmba)-induced mammary lesions in organ culture. moreover, it was an effective inhibitor against two stage skin carcinogenesis. in the present study, we synthesized several analogs of brassinin and evaluated their effectiveness in the mouse mammary gland organ culture model. results showed that cyclobrassinin, also a naturally occurring brassinin analog, w ...19948074474
determination of biogenic amines in leafy vegetables by amino acid analyser.an automated ion-exchange chromatographic method is described for determination of biogenic amines in leafy vegetables using a cation-exchange resin column in potassium form and a three-buffer system. the following amines were separated and quantified for chinese cabbage, endive, iceberg lettuce and radiocchio: putrescine, histamine, cadaverine, spermidine, agmatine, spermine, tyramine. amines from vegetable samples were extracted with 10% trichloroacetic acid. the total concentration of these a ...19948178576
high performance liquid chromatographic determination of phenolic acids in fruits and vegetables.a simple isocratic hplc technique has been developed for the quantitative analysis of phenolic acids (pas) in fruits and vegetables. nine benzoic and cinnamic acid derivatives were separated in less than 30 min, and the resolution was all more than 1.23. the ranges of linearity for pas standards were 0.2-100 ng, even up to 600 ng (r = 0.983-1.000) and the detection limits were 0.02-0.24 mg/kg. samples of fresh vegetables and fruits were extracted with 80% methanol and ethyl acetate, then purifie ...19938198755
in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline.the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ...19948206443
cauliflower mosaic virus gene i product (p1) forms tubular structures which extend from the surface of infected protoplasts.immunofluorescent staining of chinese cabbage protoplasts infected with cauliflower mosaic virus (camv) showed different patterns of staining when antibodies raised against virus particles or antibodies specific for the camv movement protein (p1) were used. anti-virion serum showed that particles accumulated as small discrete aggregates within the cytoplasm of infected protoplasts. in contrast, anti-p1 serum identified long thread-like structures extending from the protoplast surface. the same s ...19938317106
increased viral yield and symptom severity result from a single amino acid substitution in the turnip yellow mosaic virus movement protein.turnip yellow mosaic virus is a positive-strand rna virus that produces light green or yellow-green mosaic symptoms in chinese cabbage plants. we have characterized a strain that produces nearly uniform yellow-green chlorosis in systemically infected chinese cabbage leaves. the increased symptom severity is due to the single nucleotide substitution u1888-->c, which results in a tyrosine to histidine substitution in the movement protein encoded by orf-69. coding by the overlapping orf-206 is not ...19938324245
electrotransfection of turnip yellow mosaic virus rna into brassica leaf protoplasts and detection of viral rna products with a non-radioactive probe.we describe here a convenient and efficient system for studying turnip yellow mosaic virus (tymv) replication in leaf protoplasts. inoculation of rapeseed (brassica napus) or chinese cabbage (b. sinensis) protoplasts was achieved via electroporation, and sensitive detection of viral rna products was performed by northern blot analyses using a non-radioactive digoxigenin-labelled cdna probe. virus replication was detected when 1.5 x 10(6) rapeseed protoplasts were inoculated with 20 ng of tymv rn ...19938376966
characterization of a cdna encoding cysteine proteinase inhibitor from chinese cabbage (brassica campestris l. ssp. pekinensis) flower buds.a cdna encoding a new phytocystatin isotype named bcpi-1 was isolated from a cdna library of chinese cabbage flower buds. the bcpi-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. bcpi-1 has an unusually long c-terminus. a bcpi-1 fusion protein expressed in escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. genomic southern blot analysis revealed that the bcpi gene is a member of a small multi-gene family ...19968616262
[characterization of low molecular weight zinc species in normal commercial vegetable foodstuffs].in six different ordinary vegetables, namely kohlrabi, chinese cabbage, chard, leak, spinach and jerusalem artichoke, zinc was mainly found as low molecular weight species. in the present study, these important zinc compounds are further investigated. the determinations of the metal are performed by et-aas. the zinc complexes of all vegetables are anionic at ph 8.0 and show similar elution behaviour in gel permeation and anion exchange chromatography. consequently, a great resemblance in structu ...19968721222
expressed sequence tags of chinese cabbage flower bud cdna.we randomly selected and partially sequenced cdna clones from a library of chinese cabbage (brassica campestris l. ssp. pekinensis) flower bud cdnas. out of 1216 expressed sequence tags (ests), 904 cdna clones were unique or nonredundant. five hundred eighty-eight clones (48.4%) had sequence homology to functionally defined genes at the peptide level. only 5 clones encoded known flower-specific proteins. among the cdnas with no similarity to known protein sequences (628), 184 clones had signific ...19968787028
characterization of three anther-specific genes isolated from chinese cabbage.two cdna libraries were constructed from poly(a)+ rnas isolated from each of immature flowers (less than 2.0 mm long buds) and anthers (2.0-5.0 mm long buds) of chinese cabbage (brassica campestris l. ssp. pekinensis). using dot-differential hybridization, three cdna clones, designated bif38, ban54, and ban237, have been isolated from the constructed cdna libraries and sequenced completely in both directions. northern blot analyses indicate that all three cdna clones are abundantly expressed in ...19979037172
promoter sequences of two homologous pectin esterase genes from chinese cabbage (brassica campestris l. ssp. pekinensis) and pollen-specific expression of the gus gene driven by a promoter in tobacco plants.the promoter regions of two genomic clones, gban215-6 and gban215-12 from chinese cabbage (brassica campestris l. ssp. pekinensis), were sequenced. the nucleotide sequences of their promoter regions were compared with that of the bp19 pollen-specific gene of brassca napus. high nucleotide sequence homologies were observed among these three genes in the region between 210 bp upstream and the putative transcription start site. a sequence motif tgtggtg, which is similar to that of the pb core motif ...19979085260
turnip yellow mosaic virus rna-dependent rna polymerase: initiation of minus strand synthesis in vitro.an rna-dependent rna polymerase (rdrp) activity was detergent-solubilized from the chloroplast membranes of chinese cabbage leaves infected with turnip yellow mosaic virus (tymv). the template-dependent, micrococcal nuclease-treated activity synthesized full-length minus strands from tymv rna and 3'-fragments as short as a 28-nucleotide-long rna comprising the amino acid acceptor stem of the 3'-trna-like structure (tls). minus strands were shown to arise by de novo initiation with the insertion ...19979217066
a functional role for the conserved protonatable hairpins in the 5' untranslated region of turnip yellow mosaic virus rna.the 5' untranslated region (utr) of the rna of several tymoviruses contains conserved hairpins with protonatable internal loops, consisting of c-c and c-a mismatches (k. hellendoorn, p. j. a. michiels, r. buitenhuis, and c. w. a. pleij, nucleic acids res. 24, 4910-4917, 1996). here, we present a functional analysis of the 5' utr of turnip yellow mosaic virus (tymv) rna, which contains two protonatable hairpins with nearly identical internal loops. mutations were introduced in an infectious cdna ...19979343237
lifestyle and anti-helicobacter pylori immunoglobulin g antibody among outpatients.since eradication of helicobacter pylori (h. pylori) is thought to be a preventive measure against stomach cancer, several studies have examined factors associated with the infection. this paper reports the association of the infection with lifestyle factors observed in a hospital-based case-control study. cases were 140 anti-h. pylori igg antibody-positive outpatients (75 males and 65 females). controls were 52 antibody-negative outpatients (22 males and 30 females). both groups had undergone g ...19979439678
lead uptake and effects on seed germination and plant growth in a pb hyperaccumulator brassica pekinensis rupr. 19989470991
turnip yellow mosaic virus isolates with experimentally produced recombinant virion proteins.the consensus secondary structure of the virion proteins (vps) of tymoviruses was predicted from their amino acid sequences using a combination of computer methods: profile alignment, hydrophobicity moment and 'predictprotein'. all methods predicted that they were eight-stranded anti-parallel beta-barrels with two alpha-helical regions. the predicted structure was used to design recombinants of turnip yellow mosaic virus (tymv) in which selected parts of its vp were replaced with homologous regi ...19989472626
virion protein sequence variation among australian isolates of turnip yellow mosaic tymovirus.the virion protein genes, and 3' untranslated regions, of six variants of turnip yellow mosaic tymovirus (tymv) that produced different symptoms in their native host cardamine robusta and in chinese cabbage plants, have been sequenced. the sequences have been compared with each other, and with the same region of the pbl-16 clone of the blue lake isolate of tymv. the sequences of the virion protein genes differed by a mean of 1.89% (range 0-2.82%), and the encoded proteins by a mean of 1.71% (ran ...19989505977
development of a field bioassay for the side effects of pesticides on decomposition.a method has been developed for assessing the side effects of fungicides on decomposition using litterbags. twenty dried leaf disks of chinese cabbage, brassica oleracea, were placed in litterbags (20 x 20 cm) made of nylon fabric (25 microns mesh). the litterbags were laid in a treated plot and 5, 10, 20, and > 200 m from the treated plot and covered with 1 cm of standard soil. after 1 week, the dry weight of the leaf disks was determined. in an iterative procedure the method was optimized and ...20139626544
pollen ablation of transgenic tobacco plants by expression of the diphtheria toxin a-chain gene under the control of a putative pectin esterase promoter from chinese cabbage.we previously showed that a 383 bp (-274 to approximately +109) promoter of a pollen-specific gene, gban215-6, had a property of a late gene in pollen development in transgenic tobacco plants. it drove gus gene expression from uninucleate microspores to pollen tube growth of trinucleated cells. to more precisely characterize the specificity of the promoter, we placed the diphtheria toxin a-chain (dtx-a) coding region under the control of the gban215-6 promoter. transgenic tobacco plants containi ...19989666468
diet and brain cancer in adults: a case-control study in northeast china.a hospital-based case-control study was conducted in the heilongjiang province of northeast china between may 1993 and may 1995. a total of 129 histologically confirmed brain cancer cases (73 gliomas and 56 meningiomas) and 258 matched controls were interviewed in 6 major hospitals to examine the influence of dietary factors in developing brain cancer. information was obtained about frequency of consumption of 57 food items. odds ratios (ors) were obtained from conditional logistic regression, i ...199910077146
evaluation of spinning disc technology for the application of entomopathogenic nematodes against a foliar pest.two spinning disc spray application systems, the micron herbaflex and micron ulva+, were assessed for their potential for the application of infective juveniles (ijs) of entomopathogenic nematodes (epns) against larvae of the diamondback moth (dbm), plutella xylostella. the effect of initial concentration of ijs on subsequent infection was examined for three species of epns: steinernema sp. (m87), steinernema sp. (ssl85), and heterorhabditis sp. increasing the concentration of ijs generally resu ...199910222182
cloning and expression of a new isotype of the peroxiredoxin gene of chinese cabbage and its comparison to 2cys-peroxiredoxin isolated from the same plant.a cdna encoding a newly identified isotype of peroxiredoxin (prx) was isolated from a chinese cabbage flower bud cdna library and designated cprxii. database searches using the predicted cprxii amino acid sequence revealed no substantial homology to other proteins with the exception of the yeast type ii prx with which cprxii shares 27.8% sequence identity. recombinant cprxii expressed in escherichia coli was able to protect glutamine synthetase from inactivation in a metal-catalyzed oxidation sy ...199910329461
zinc-finger proteins: the classical zinc finger emerges in contemporary plant science.tfiiia-type zinc fingers have been found in a number of eucaryotic transcription factors as dna-binding motifs. in plants, as many as 30 proteins have been reported that have either one, two, three or four zinc fingers. plant zinc-finger proteins are characterized by long spacers of diverse lengths between adjacent fingers and a highly conserved sequence, qalggh, located within a putative dna-contacting surface of each finger. in vitro dna-binding experiments with two-fingered proteins of petuni ...199910380795
[effect of green vegetable on digestive tract absorption of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in rats].the effect green vegetable on fecal excretion of polychlorinated dibenzo-p-dioxin (pcdd) congeners and polychlorinated dibenzofuran (pcdf) congeners was examined in male rats. the rats were administered 10% vegetable diets or a basal diet containing 0.2 ml of the causal rice-bran oil of yusho that had occurred in the southwest part of japan in 1968 and kept on the same diet for five days. the fecal excretion of 2,3,7,8-t4cdd and 2,3,4,7,8-p5cdf in the group fed with komatsuna, mitsuba, spinach a ...199910396873
molecular cloning, expression, and functional characterization of a 2cys-peroxiredoxin in chinese cabbage.a cdna (c2c-prx) corresponding to a 2cys-peroxiredoxin (2cys-prx) was isolated from a leaf cdna library of chinese cabbage. the predicted amino acid sequence of c2c-prx has 2 conserved cysteines and several peptide domains present in most of the 2cys-prx subfamily members. it shows the highest sequence homology to the 2cys-prx enzymes of spinach (88%) and arabidopsis (86%). southern analysis using the cdna insert of c2c-prx revealed that it consists of a small multigene family in chinese cabbage ...199910487217
nitrate and nitrite in vegetables on the danish market: content and intake.the contents of nitrate and nitrite in lettuce, leek, potato, beetroot, chinese cabbage and white cabbage on the danish market were determined for 3 years in the period 1993-1997 as part of the danish food monitoring programme. these vegetables are supposed to provide the major contribution to the intake of nitrate from the diet. results for nitrate and nitrite in fresh and frozen spinach are also shown. the highest content of nitrate was found in lettuce followed by beetroot, chinese cabbage, f ...199910656054
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