Publications

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thynnascaris rhacodes sp. n. (nematoda: ascaridoidea) in fishes from the israeli mediterranean coast.thynnascaris rhacodes sp. n. infects pelates quadrilineatus, solea vulgaris, boops boops, lithognathus mormyrus, obleida melanura, diplodus vulgaris, d. sargus, and sparus auratus from the eastern mediterranean sea. unique in that the cuticle on the female tail is wrinkled, the ascaridoid is further characterized by possessing 27 to 35 precloacal papillae, a body length of 11 to 85 mm, similar spicules 3 to 6% of body length, a length-ratio of intestinal cecum to ventricular appendage of 1 : 1 t ...2003570370
evaluating molar cyp1a level in fish hepatic microsomes by competitive elisa using recombinant membrane-free cyp1a standard protein.fish cytochrome p4501a (cyp1a) is a widely accepted environmental biomarker, detecting biological effects of several xenobiotic groups present in aquatic environments, when evaluated in target tissues of a biosensor species. however, appropriate utilization of its protein level as a routine environmental diagnostic tool requires evaluation of properly normalized molar levels, mitigating comparison among different laboratories, during a multi-annual time scale and over a variety of tested populat ...200212088637
quantitative evaluation of hepatic cytochrome p4501a transcript, protein, and catalytic activity in the striped sea bream (lithognathus mormyrus).hepatic cytochrome p4501a (cyp1a) expression was partially characterized in the striped sea bream (lithognathus mormyrus) from the mediterranean coast of israel as part of the process of establishing the cyp1a gene as an environmental biomarker. reverse transcription-competitive polymerase chain reaction, competitive enzyme-linked immunosorbent assay, and ethoxyresorufin o-deethylase (erod) assay were used for evaluating transcript, protein, and catalytic activity levels, respectively, in absolu ...200312959535
discord in the family sparidae (teleostei): divergent phylogeographical patterns across the atlantic-mediterranean divide.the strait of gibraltar has been proposed to be the divide between two marine biogeographical regions, the mediterranean sea and the northeast atlantic. intraspecific studies have shown, for several of the examined species, a reduction of gene flow between the two basins. the present study examines genetic variation at nuclear and mitochondrial loci in five marine teleost species belonging to the family sparidae. four samples for each species were analysed spanning the northeast atlantic and the ...200314640406
application of real-time pcr for quantitative determination of hepatic vitellogenin transcript levels in the striped sea bream, lithognathus mormyrus.the striped sea bream (lithognathus mormyrus) has been recently introduced as a bioindicator fish species in mediterranean coastal habitats. the purpose of this study was to apply a real-time pcr assay for the determination of absolute levels of hepatic vtg transcript in this fish, identifying minimal and maximal levels and establishing the relationship between vtg rna levels and ovarian development. a partial vtg cdna was cloned from hepatic rna of a striped sea bream female. specific primers w ...201515178095
life-history- and ecosystem-driven variation in composition and residence pattern of seabream species (perciformes: sparidae) in two mediterranean coastal lagoons.species composition and length-frequency distributions of six sparid fish species were investigated in two central mediterranean coastal lagoons off the western coast of italy: fogliano and caprolace. in the former, the sparid fauna was dominated by the gilt-head seabream (sparus aurata), whereas in caprolace, species composition was more homogeneous across all six species. size structure varied considerably among species: s. aurata, diplodus puntazzo and diplodus vulgaris had a single-cohort st ...200616266729
biomonitoring study of heavy metals in biota and sediments in the south eastern coast of mediterranean sea, egypt.concentrations of cd, cu, co, zn, mn and fe were determined in biota and sediment samples collected from the eastern harbour and el-mex bay in the mediterranean sea, egypt. the levels of cu, co, zn, mn and fe in the macroalgae, ulva lactuca, enteromorpha compressa (green algae) and jania rubens (red algae), recorded high concentrations except for cd. moreover, fe was the most predominant metal in the seaweed. the two species of bivalves, donax trunculus and paphia textile, showed different amoun ...200818074236
molecular mechanisms determining sperm motility initiation in two sparids (sparus aurata and lithognathus mormyrus).molecular mechanisms involved in sperm motility initiation in two sparids (sparus aurata and lithognathus mormyrus) have been studied. our comparative study demonstrates that osmolality is the key signal in sperm motility activation in both species, whereas k(+) and ca(2+) do not have any role. the straight-line velocity that resulted, however, was significantly different when measured in sperm activated with non-ionic and/or calcium-free solutions with respect to that measured in seawater-activ ...200818417709
morphological and molecular redescription of the myxozoan unicapsula pflugfelderi schubert, sprague & reinboth 1975 from two teleost hosts in the mediterranean. a review of the genus unicapsula davis 1924.elongate plasmodia with myxosporean spores belonging to the genus unicapsula, davis, 1924 were found in the skeletal muscle of the striped seabream, lithognathus mormyrus (l.), a candidate for the mediterranean aquaculture. the only species of unicapsula described from the mediterranean is unicapsula pflugfelderi schubert et al. 1975, which occurs in the picarel, spicara smaris (l.). for morphological and molecular comparison of u. pflugfelderi from s. smaris with unicapsula sp. from l. mormyrus ...200919236560
diplectanid parasites of lithognathus mormyrus (l.) (teleostei: sparidae) from the mediterranean sea, with the description of lamellodiscus flagellatus n. sp. (monogenea: diplectanidae).species of lamellodiscus johnston & tiegs, 1922 are described from the gills of lithognathus mormyrus (linnaeus) in the mediterranean sea. a new nomenclature for a male copulatory organ with two articulated pieces is proposed: the hollow piece is referred to as the penis and we propose the term 'vectis' for the entirely sclerotised piece. it is shown that l. verberis euzet & oliver, 1967 actually comprised two species, l. verberis (sensu stricto), which is redescribed based on both the type-mate ...200919731099
the effect of tert-butyl hydroperoxide on hepatic transcriptome expression patterns in the striped sea bream (lithognathus mormyrus; teleostei).the study was aimed at examining the effects of tert-butyl hydroperoxide (tbhp) on hepatic transcriptome expression patterns of the teleost fish lithognathus mormyrus. tbhp is an organic hydro-peroxide, widely used as a model pro-oxidant. it generates the reactive oxygen species (ros) tert-butoxyl and tert-butylperoxyl. complementary dnas of tbhp-treated vs control fish were applied onto a previously produced cdna microarray of approximately 1500 unique sequences. the effects of the tbhp applica ...201020553222
copper induces cu-atpase atp7a mrna in a fish cell line, saf1.copper transporting atpase, atp7a, is an atp dependent copper pump present in all vertebrates, critical for the maintenance of intracellular and whole body copper homeostasis. effects of copper treatment on atp7a gene expression in fibroblast cells (saf1) of the sea bream (sparus aurata) were investigated by qrt-pcr and by a medium density microarray from a closely related species, striped sea bream (lithognathus mormyrus). to discriminate between the effects of cu and other metals, saf1 cells w ...201121473932
differentiation of sparidae species by dna sequence analysis, pcr-sscp and ief of sarcoplasmic proteins.the increasing global trade of fishery and aquaculture products makes it necessary to develop methods for species identification in case of fish fillets or other highly processed seafood with external morphological characteristics (e.g. gills, fins) of the original fish being removed. species identification methods based on dna- or protein-analysis, like dna sequencing of pcr products, pcr-sscp (polymerase chain reaction - single strand conformation polymorphism), and isoelectric focusing (ief) ...201323265470
molecular evidence for the existence of species complexes within macvicaria gibson & bray, 1982 (digenea: opecoelidae) in the western mediterranean, with descriptions of two new species.morphological and molecular characterisation of specimens of macvicaria spp. (digenea: opecoelidae) from five species of mediterranean sparids (teleostei: sparidae) sampled in the bizerte lagoon and the bay of bizerte (tunisia) revealed the presence of two species complexes designated as "obovata" and "crassigula" groups. detailed comparative morphological and phylogenetic analyses revealed that two of the specimen sets, one from each complex, represent species new to science. m. maamouriae n. s ...201526063299
potential health hazard assessment in terms of some heavy metals determined in demersal fishes caught in eastern aegean sea.a heavy metal risk assessment based on estimated daily intake (edi) and target hazard quotient was made for children and adults. five fish species captured from the eastern aegean sea were analyzed for cr, cu, cd, hg and pb by inductively coupled plasma spectroscopy in muscle tissue of red mullet (mullus barbatus), surmullet (mullus surmuletus), sand steenbras (lithognathus mormyrus), common two-banded seabream (diplodus vulgaris) and common pandora (pagellus erythinus). the ranges for mean meta ...201526109309
morphology and molecular analysis of life-cycle stages of proctoeces maculatus (looss, 1901) (digenea: fellodistomidae) in the bizerte lagoon, tunisia.the life cycle of proctoeces maculatus (looss, 1901) (digenea, fellodistomidae) was studied in bizerte lagoon (tunisia). three sequential hosts appear to be involved: the mediterranean mussel mytilus galloprovincialis lamarck, 1819 (mytilidae) as the first intermediate host; the polychaete sabella pavonina savigny, 1822 (sabellidae), as the second intermediate host; and fishes (lithognathus mormyrus (linnaeus, 1758) (sparidae), trachinotus ovatus (linnaeus, 1758) (carangidae) and sparus aurata l ...201626694018
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