Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| rapid identification of bacteria of the comamonadaceae with amplified ribosomal dna-restriction analysis (ardra). | ribosomal rrna gene fragments (rdna) encompassing the 16s rdna, the 16s-23s rdna spacer region and part of the 23s rdna of 95 strains belonging to 13 well-described taxa of the eubacterial family comamonadaceae (beta subclass of the proteobacteria or rrna superfamily iii) were enzymatically amplified using conserved primers. the fragments of approximately 2400 base pairs were subjected to restriction analysis. restriction fragment length patterns obtained with hinfi enabled us to distinguish 9 o ... | 1992 | 1354195 |
| characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (pcb)-degrading strains of comamonas testosteroni isolated from pcb-contaminated soil. | in this study, we isolated and characterized biphenyl (bp) and polychlorinated biphenyl (pcb) degrading bacterial strains found in pcb-contaminated soil from an auto manufacturing plant located in syracuse, new york. twenty-one bp and pcb-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. of the 21 bacteria, 13 were identified as comamonas testosteroni, constituting about 60% of the bacterial population examined. other pcb d ... | 1995 | 7641143 |
| metabolic relationship between the co dehydrogenase molybdenum cofactor and the excretion of urothione by hydrogenophaga pseudoflava. | urothione was isolated as an excretion product of hydrogenophaga pseudoflava and other bacteria at amounts approaching 253 micrograms/l of culture corresponding to 44 micrograms/g bacterial dry mass. the compound was identified as urothione by co-chromatography with urothione isolated from human urine, its characteristic ultraviolet and visible absorption spectra, oxidation to pterin-6-carboxylic-7-sulfonic acid by alkaline permanganate, 1h-nmr spectroscopy, double-quantum-filtered fourier-trans ... | 1994 | 7957196 |
| influence of temperature on growth rate and competition between two psychrotolerant antarctic bacteria: low temperature diminishes affinity for substrate uptake. | the growth kinetics of two psychrotolerant antarctic bacteria, hydrogenophaga pseudoflava cr3/2/10 (2/10) and brevibacterium sp. strain cr3/1/15 (1/15), were examined over a range of temperatures in both batch culture and glycerol-limited chemostat cultures. the maximum specific growth rate (mu max) and ks values for both bacteria were functions of temperature, although the cell yields were relatively constant with respect to temperature. the mu max values of both strains increased up to an opti ... | 1994 | 8031092 |
| influence of changing temperature on growth rate and competition between two psychrotolerant antarctic bacteria: competition and survival in non-steady-state temperature environments. | competition between two psychrotolerant bacteria was examined in glycerol-limited chemostat experiments subjected to non-steady-state conditions of temperature. one bacterium, a brevibacterium sp. strain designated cr3/1/15, responded rapidly to temperature change, while a second, hydrogenophaga pseudoflava, designated cr3/2/10, exhibited a lag in growth after a shift-down during a square-wave temperature cycle but not after a shift-up. the effects on competition and survival by these bacteria o ... | 1994 | 8031093 |
| syntrophic interactions during degradation of 4-aminobenzenesulfonic acid by a two species bacterial culture. | during synthrophic growth of hydrogenophaga palleronii (strain s1) and agrobacterium radiobacter (strain s2) with 4-aminobenzene sulfonate (4abs) only strain s1 desaminates 4abs by regioselective 3,4-dioxygenation. the major part of the metabolite catechol-4-sulfonate (4cs) is excreted and further metabolized by strain s2. although both organisms harbour activities of protocatechuate pathways assimilation of the structural analog 4cs requires first of all enzyme activities with broader substrate ... | 1993 | 8439234 |
| purification and characterization of a novel type of protocatechuate 3,4-dioxygenase with the ability to oxidize 4-sulfocatechol. | 4-aminobenzenesulfonate is degraded via 4-sulfocatechol by a mixed bacterial culture that consists of hydrogenophaga palleronii strain s1 and agrobacterium radiobacter strain s2. from the 4-sulfocatechol-degrading organism a. radiobacter strain s2, a dioxygenase that converted 4-sulfocatechol to 3-sulfomuconate was purified to homogeneity. the purified enzyme also converted protocatechuate with a similar catalytic activity to 3-carboxy-cis, cis-muconate. furthermore, the purified enzyme oxidized ... | 1996 | 8772173 |
| degradation of 4-aminobenzenesulfonate by a two-species bacterial coculture. physiological interactions between hydrogenophaga palleronii s1 and agrobacterium radiobacter s2. | the mutualistic interactions in a 4-aminobenzenesulfonate (sulfanilate) degrading mixed bacterial culture were studied. this coculture consisted of hydrogenophaga palleronii strain s1 and agrobacterium radiobacter strain s2. in this coculture only strain s1 desaminated sulfanilate to catechol-4-sulfonate, which did not accumulate in the medium but served as growth substrate for strain s2. during growth in batch culture with sulfanilate as sole source of carbon, energy, nitrogen and sulfur, the r ... | 1996 | 8782393 |
| purification and some properties of ribulose 1,5-bisphosphate carboxylases/oxygenases from acinetobacter sp. strain jc1 and hydrogenophaga pseudoflava. | ribulose 1,5-bisphosphate carboxylases/oxygenases (rubiscos) of two carboxydobacteria, acinetobacter sp. strain jc1 and hydrogenophaga pseudoflava, grown on carbon monoxide were purified and partially characterized. rubisco of acinetobacter sp. jc1 was purified 5-fold in eight steps to homogeneity, with a yield of 1.6%. the final specific activity of the purified enzyme was 39.5 nmol co2 incorporated per min per mg protein. the molecular weight of the native enzyme was determined to be 520,000. ... | 1997 | 9264026 |
| purification and characterization of a manganese-containing superoxide dismutase from a carboxydobacterium, pseudomonas carboxydohydrogena. | superoxide dismutase from pseudomonas carboxydohydrogena, a carboxydobacterium, grown on carbon monoxide was purified 37.6-fold in seven steps to homogeneity, with a yield of 1.4%. the final specific activity was 2,396 units per mg protein as determined by an assay based on a 50% decrease in the rate of cytochrome c reduction. the molecular weight of the native enzyme was determined to be 42,500. sodium dodecyl sulfate gel electrophoresis revealed two identical subunits of molecular weight 21,70 ... | 1997 | 9509413 |
| isolation of a bacterial strain with the ability to utilize the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy. | a bacterial strain (strain s5) which grows aerobically with the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy was isolated. this strain was obtained by continuous adaptation of "hydrogenophaga palleronii" s1, which has the ability to grow aerobically with 4-aminobenzenesulfonate. strain s5 probably cleaves 4-carboxy-4'-sulfoazobenzene reductively under aerobic conditions to 4-aminobenzoate and 4-aminobenzene-sulfonate, which are mineralized by previ ... | 1998 | 9603860 |
| microbial communities of printing paper machines. | the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ... | 1998 | 9717292 |
| effect of molybdate and tungstate on the biosynthesis of co dehydrogenase and the molybdopterin cytosine-dinucleotide-type of molybdenum cofactor in hydrogenophaga pseudoflava. | the molybdenum-containing iron-sulfur flavoprotein co dehydrogenase is expressed in a catalytically fully competent form during heterotrophic growth of the aerobic bacterium hydrogenophaga pseudoflava with pyruvate plus co. we have adopted these conditions for studying the effect of molybdate (mo) and tungstate (w) on the biosynthesis of co dehydrogenase and its molybdopterin (mpt) cytosine-dinucleotide-(mcd)-type molybdenum cofactor. w was taken up by the mo transport system and, therefore, int ... | 1998 | 9738918 |
| cloning and characterization of ribulose bisphosphate carboxylase gene of a carboxydobacterium, hydrogenophagea pseudoflava dsm 1084. | the ribulose bisphosphate carboxylase/oxygenase rbcl and rbcs genes of a carbon monoxide-oxidizing bacterium, hydrogenophaga pseudoflava dsm 1084, were cloned and sequenced. the cloned rbcl and rbcs genes had open reading frames of 1422 and 351 nucleotides encoding rbcl and rbcs with calculated molecular masses of 52,689 and 13,541, respectively. the known active site residues in other rbcl proteins were conserved in the h. pseudoflava proteins. the h. pseudoflava rbcs protein lacked the 12-resi ... | 1998 | 9856338 |
| production of poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid) and poly(4-hydroxybutyric acid) without subsequent degradation by hydrogenophaga pseudoflava. | a hydrogenophaga pseudoflava strain was able to synthesize poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid) [p(3hb-co-4hb)] having a high level of 4-hydroxybutyric acid monomer unit (4hb) from gamma-butyrolactone. in a two-step process in which the first step involved production of cells containing a minimum amount of poly(3-hydroxybutyric acid) [p(3hb)] and the second step involved polyester accumulation from the lactone, approximately 5 to 10 mol% of the 3-hydroxybutyric acid (3hb) derived ... | 1999 | 10103252 |
| phylogenetic relationships among members of the comamonadaceae, and description of delftia acidovorans (den dooren de jong 1926 and tamaoka et al. 1987) gen. nov., comb. nov. | the phylogenetic relationships among members of the family comamonadaceae and several unclassified strains were studied by direct sequencing of their pcr-amplified 16s rrna genes. based on the 16s rrna gene sequence analysis, members of the family formed a coherent group. the closest relatives are species of the rubrivivax sub-group: leptothrix discophora, ideonella dechloratans and rubrivivax gelatinosus. the genus hydrogenophaga formed two subclusters, as did the species of acidovorax, whereas ... | 1999 | 10319477 |
| sequence analysis, characterization and co-specific transcription of the cox gene cluster on the megaplasmid phcg3 of oligotropha carboxidovorans. | sequence, transcriptional, mutational and physiological analyses indicate that the carbon monoxide (co) dehydrogenase of oligotropha carboxidovorans is an integral and unique part of an elaborate co oxidizing system. it is encoded by the 14.5kb gene cluster coxbcmsldefghik residing on the 128kb megaplasmid phcg3. the co dehydrogenase structural genes coxmsl are flanked by nine accessory genes arranged as the cox gene cluster. the cox genes are specifically and coordinately transcribed under chem ... | 1999 | 10433972 |
| cloning and molecular characterization of the genes for carbon monoxide dehydrogenase and localization of molybdopterin, flavin adenine dinucleotide, and iron-sulfur centers in the enzyme of hydrogenophaga pseudoflava. | carbon monoxide dehydrogenases (co-dh) are the enzymes responsible for the oxidation of co to carbon dioxide in carboxydobacteria and consist of three nonidentical subunits containing molybdopterin flavin adenine dinucleotide (fad), and two different iron-sulfur clusters (o. meyer, k. frunzke, d. gadkari, s. jacobitz, i. hugendieck, and m. kraut, fems microbiol. rev. 87:253-260, 1990). the three structural genes of co-dh in hydrogenophaga pseudoflava were cloned and characterized. the genes were ... | 1999 | 10482497 |
| local sequence dependence of polyhydroxyalkanoic acid degradation in hydrogenophaga pseudoflava. | the first order intracellular degradation of various polyhydroxyalkanoic acid (pha) inclusions in hydrogenophaga pseudoflava cells was investigated by analyzing the compositional and microstructural changes of the pha using gas chromatography, (13)c nmr spectroscopy, and differential scanning calorimetry. two types of pha, copolymers and blend-type polymers, were separately accumulated in cells for comparison. the constituent monomers were 3-hydroxybutyric acid (3hb), 4-hydroxybutyric acid (4hb) ... | 1999 | 10608842 |
| aerobic degradation of dinitrotoluenes and pathway for bacterial degradation of 2,6-dinitrotoluene. | an oxidative pathway for the mineralization of 2,4-dinitrotoluene (2, 4-dnt) by burkholderia sp. strain dnt has been reported previously. we report here the isolation of additional strains with the ability to mineralize 2,4-dnt by the same pathway and the isolation and characterization of bacterial strains that mineralize 2, 6-dinitrotoluene (2,6-dnt) by a different pathway. burkholderia cepacia strain js850 and hydrogenophaga palleronii strain js863 grew on 2,6-dnt as the sole source of carbon ... | 2000 | 10788393 |
| the effect of intracellular molybdenum in hydrogenophaga pseudoflava on the crystallographic structure of the seleno-molybdo-iron-sulfur flavoenzyme carbon monoxide dehydrogenase. | crystal structures of carbon monoxide dehydrogenase (codh), a seleno-molybdo-iron-sulfur flavoprotein from the aerobic carbon monoxide utilizing carboxidotrophic eubacterium hydrogenophaga pseudoflava, have been determined from the enzyme synthesized at high (mo(plus) codh) and low intracellular molybdenum content (mo(minus) codh) at 2.25 a and 2.35 a resolution, respectively. the structures were solved by patterson search methods utilizing the enzyme from oligotropha carboxidovorans as the init ... | 2000 | 10966817 |
| the role of se, mo and fe in the structure and function of carbon monoxide dehydrogenase. | co dehydrogenase (ec 1.2.99.2) catalyzes the oxidation of co according to the following equation: co + h2o-->co2 + 2 e- + 2 h+. it is a selenium-containing molybdo-iron-sulfur-flavoenzyme, which has been crystallized and structurally characterized in its oxidized state from the aerobic co utilizing bacteria oligotropha carboxidovorans and hydrogenophaga pseudoflava. both co dehydrogenase structures show only minor differences, and the enzymes are dimers of two heterotrimers. each heterotrimer is ... | 2000 | 11076018 |
| alpha- and beta-proteobacteria control the consumption and release of amino acids on lake snow aggregates. | we analyzed the composition of aggregate (lake snow)-associated bacterial communities in lake constance from 1994 until 1996 between a depth of 25 m and the sediment surface at 110 m by fluorescent in situ hybridization with rrna-targeted oligonucleotide probes of various specificity. in addition, we experimentally examined the turnover of dissolved amino acids and carbohydrates together with the microbial colonization of aggregates formed in rolling tanks in the lab. generally, between 40 and m ... | 2001 | 11157226 |
| hydrogenophaga intermedia sp. nov., a 4-aminobenzenesulfonate degrading organism. | the taxonomic status of a gram-negative, oxidase positive rod (strain s1) able to degrade 4-aminobenzenesulfonate was studied using a polyphasic approach. chemotaxonomic investigations of quinones and polar lipids established the allocation of this strain to the beta-subclass of the proteobacteria and revealed similarities to hydrogenophaga palleronii. 16s rrna sequence comparisons demonstrated that this strain clusters phylogenetically with h. palleronii and h. taeniospiralis, but clearly repre ... | 2000 | 11249018 |
| retention of water-borne bacteria by membrane filters. part iii: bacterial challenge tests on 0.1 micron rated filters. | clear performance differences were observed between different 0.1 micron rated filters in terms of their microbial removal efficiency when challenged with naturally occurring waterborne bacteria from a water source. penetration occurred with three 0.1 micron rated "sterilizing grade" filter types tested, from three different filter manufacturers, that did not have a specific high titer reduction claim for acholeplasma laidlawii. bacteria shown to penetrate these 0.1 micron rated filters were qui ... | 2001 | 11310317 |
| retention of water-borne bacteria by membrane filters. part ii: scanning electron microscopy (sem) and fatty acid methyl ester (fame) characterization of bacterial species recovered downstream of 0.2/0.22 micron rated filters. | the results of scanning electron microscopic (sem) and fatty acid methyl ester (fame) characterization of the bacterial species shown to penetrate conventional 0.2/0.22 micron rated "sterilizing grade" filters are presented. sem data suggest that retention of bacteria by these filters appears to be strongly influenced by the morphology, and especially the width of bacteria and less so by length. when the bacterial cell width is small, less than 0.3 micron or so, the cell length does not appear t ... | 2001 | 11310323 |
| cloning of the genes for a 4-sulphocatechol-oxidizing protocatechuate 3,4-dioxygenase from hydrogenophaga intermedia s1 and identification of the amino acid residues responsible for the ability to convert 4-sulphocatechol. | the genes for a protocatechuate 3,4-dioxygenase (p34o-ii) with the ability to oxidize 4-sulphocatechol were cloned from the 4-aminobenzenesulphonate(sulphanilate)-degrading bacterium hydrogenophaga intermedia strain s1 (dsmz 5680). sequence comparisons of the deduced amino acid sequences of both subunits of the p34o-ii from h. intermedia s1 (pcah-ii and pcag-ii) with those of another p34o-ii, previously obtained from agrobacterium radiobacter s2, and the corresponding sequences from the protocat ... | 2001 | 11454212 |
| xenophilus azovorans gen. nov., sp. nov., a soil bacterium that is able to degrade azo dyes of the orange ii type. | the taxonomy of strain kf46ft, which was isolated previously after an aerobic enrichment with the azo compound 1-(4'-carboxyphenylazo)-2-naphthol as the sole source of energy and carbon, was investigated by a polyphasic approach. the organism contained a quinone system with ubiquinone q-8 and 2-hydroxyputrescine and putrescine as the major polyamines, suggesting that strain kf46ft belonged to the beta-subclass of the proteobacteria. the polar lipid profile consisted mainly of phosphatidylethanol ... | 2001 | 11594616 |
| biodegradation of methyl tert-butyl ether by a pure bacterial culture. | biodegradation of methyl tert-butyl ether (mtbe) by the hydrogen-oxidizing bacterium hydrogenophaga flava env735 was evaluated. env735 grew slowly on mtbe or tert-butyl alcohol (tba) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the addition of a small amount of yeast extract. the addition of h(2) did not enhance or diminish mtbe degradation by the strain, and mtbe was only poorly degraded or not degraded by type strains of hydrogenophaga or hydroge ... | 2001 | 11722912 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part i: characterization of water isolates for potential use as standard challenge organisms to qualify 0.1 micron rated filters. | although 0.1 microm rated filters intended for pharmaceutical sterilization applications have been commercially available for at least 15 years, there is no industry-wide standard for qualifying the microbial removal performance of these filters. in this article, we report on the bacterial challenge methodology used to screen four bacterial species for potential utility as a standard challenge organism to qualify 0.1 microm rated filters. these isolates were, in their natural state, demonstrated ... | 2001 | 11766822 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part ii: preliminary characterization of hydrogenophaga (formerly pseudomonas) pseudoflava for use as a standard challenge organism to qualify 0.1 micron rated filters. | in this article, we report on the preliminary characterization of hydrogenophaga (formerly pseudomonas) pseudoflava for potential use as a standard challenge organism to qualify 0.1 microm rated filters. filter-cloned h. pseudoflava (atcc 700892) was easily cultured in a low nutrient broth (r2a broth) under standard laboratory conditions, reaching high titers of 10(8)-10(9) cfu/ml within 48-65 hours of incubation at 25+/-5 degrees c. under these conditions, h. pseudoflava is a rod-shaped bacteri ... | 2001 | 11766823 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part iii: bacterial challenge tests on 0.2/0.22 and 0.1 micron rated filter cartridges with hydrogenophaga (formerly pseudomonas) pseudoflava. | we have previously reported on the preliminary characterization of hydrogenophaga (formerly pseudomonas) pseudoflava for potential use as a standard challenge organism to qualify 0.1 microm rated filters. this article reports on the retention efficiencies of a large panel of 0.2/0.22 microm and 0.1 microm rated filter cartridges for h. pseudoflava (atcc 700892) versus the retention capabilities of the same filters for brevundimonas diminuta (atcc 19146). a total of thirty-two 0.2/0.22 microm rat ... | 2001 | 11766824 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part iv: retention of hydrogenophaga pseudoflava (atcc 700892) and ralstonia pickettii (atcc 700591) by 0.2 and 0.22 micron rated filters. | ralstonia pickettii has emerged as a bioburden microorganism of considerable importance in pharmaceutical processes utilizing conventional 0.2 or 0.22 micron rated "sterilizing grade" filters. in this article, we re-evaluated and studied the retention efficiencies of 0.2 micron rated nylon 6.6 and 0.22 microns rated modified polyvinylidene fluoride (pvdf) filters for hydrogenophaga pseudoflava (atcc 700892) and r. pickettii (atcc 700591). out of a total of forty-four 0.2/0.22 micron rated filter ... | 2002 | 12109335 |
| enhancing transport of hydrogenophaga flava env735 for bioaugmentation of aquifers contaminated with methyl tert-butyl ether. | the gasoline oxygenate methyl tert-butyl ether (mtbe) has become a widespread contaminant in groundwater throughout the united states. bioaugmentation of aquifers with mtbe-degrading cultures may be necessary to enhance degradation of the oxygenate in some locations. however, poor cell transport has sometimes limited bioaugmentation efforts in the past. the objective of this study was to evaluate the transport characteristics of hydrogenophaga flava env735, a pure culture capable of growth on mt ... | 2002 | 12406751 |
| biosynthesis and local sequence specific degradation of poly(3-hydroxyvalerate-co-4-hydroxybutyrate) in hydrogenophaga pseudoflava. | a novel copolymer that consisted of 3-hydroxyvalerate and 4-hydroxybutyrate, p(3hv-co-4hb), was synthesized in hydrogenophaga pseudoflava by growing it in media containing gamma-valerolactone and gamma-butyrolactone as a carbon source. the monomer ratio in the copolymer was changed by altering the feed ratio of the two lactones. the cultivation technique was composed of three steps: the first-step for high cell production in luria-bertani medium, the second-step for intracellular degrading remov ... | 2003 | 12523844 |
| ramlibacter tataouinensis gen. nov., sp. nov., and ramlibacter henchirensis sp. nov., cyst-producing bacteria isolated from subdesert soil in tunisia. | ramlibacter gen. nov. is proposed for two aerobic, chemo-organotrophic, cyst-producing soil bacterial strains. these bacteria are gram-negative, non-flagellated rods or cysts, isolated from subdesert soil in tataouine, tunisia. phylogenetic analyses of the rrs sequences of the two strains showed that they do not constitute a robust clade at the genus level with any previously described bacteria and that they are a deep branch of a clade also grouping the genera acidovorax and hydrogenophaga with ... | 2003 | 12710631 |
| diversity and seasonal variability of beta-proteobacteria in biofilms of polluted rivers: analysis by temperature gradient gel electrophoresis and cloning. | the beta-subgroup of the proteobacteria has been shown to be important in aquatic habitats and was investigated in depth here by molecular 16s rrna techniques in biofilms of the elbe river and its polluted tributary, the spittelwasser river. the bacterial 16s rrna genes were cloned from each site, screened for beta-proteobacterial clones and sequenced. river biofilm clones from both rivers grouped into 9 clusters (rbfs). rbfs 1, 2, and 3 fell into the recently described betai cluster of cosmopol ... | 2003 | 12902230 |
| regioselective hydroxylation of quinolinic acid, lutidinic acid and isocinchomeronic acid by resting cells of pyridine dicarboxylic acid-degrading microorganisms. | microorganisms aerobically degrading quinolinic acid, lutidinic acid or isocinchomeronic acid were isolated and the microbial regioselective hydroxylation of these pyridine dicarboxylic acids was studied. alcaligenes sp. uk21 cells converted quinolinic acid into 6-hydroxypicolinic acid, suggesting the involvement of two enzyme reactions catalyzing hydroxylation at position c6 and decarboxylation at position c3 of quinolinic acid. resting cells of alcaligenes sp. uk21 accumulated 94.9 mm 6-hydrox ... | 2003 | 12955355 |
| isolation and molecular characterization of thiosulfate-oxidizing bacteria from an italian rice field soil. | in rice paddy soils an active cycling of sulfur compounds takes place. to elucidate the diversity of thiosulfate-oxidizing bacteria these organisms were enriched from bulk soil and rice roots by the most probable number method in liquid medium. from the mpn enrichment cultures 21 bacterial strains were isolated on solid mineral medium, and could be further shown to produce sulfate from thiosulfate. these strains were characterized by 16s rdna analyses. the isolates were affiliated to seven diffe ... | 2003 | 14529188 |
| bacterial composition of the biofilm on the surface of course sediment of the danube: with special reference to the clinically important bacteria. | on monthly intervals and over a period of 14 months (november, 1993-december, 1994) biofilm samples from sediments taken at the szentendre island on the danube were culturel and the isolated organisms were examined macromorphologically and micromorphogically and tested for oxidase and catalase production and their ability to oxidise and ferment glucose. the majority (85%) of the strains isolated were catalase positive. 43% were oxidase positive, 38% were glucose oxidisers and only 19% fermented ... | 1998 | 14666747 |
| arsenite oxidation by the heterotroph hydrogenophaga sp. str. nt-14: the arsenite oxidase and its physiological electron acceptor. | heterotrophic arsenite oxidation by hydrogenophaga sp. str. nt-14 is coupled to the reduction of oxygen and appears to yield energy for growth. purification and partial characterization of the arsenite oxidase revealed that it (1). contains two heterologous subunits, aroa (86 kda) and arob (16 kda), (2). has a native molecular mass of 306 kda suggesting an alpha(3)beta(3) configuration, and (3). contains molybdenum and iron as cofactors. although the hydrogenophaga sp. str. nt-14 arsenite oxidas ... | 2004 | 15178476 |
| mtbe biodegradation in a gravity flow, high-biomass retaining bioreactor. | the aerobic biodegradation of methyl tert-butyl ether (mtbe), a widely used fuel oxygenate, was investigated using a pilot-scale biomass-retaining bioreactor called a biomass concentrator reactor (bcr). the reactor was operated for a year at a flow rate of 2500 l/d on cincinnati dechlorinated tap water and an influent mtbe concentration of 5 mg/l. treatment efficiency of mtbe in the reactor during stable operations exceeded 99.9%. the upper 95% confidence levels of effluent mtbe concentrations a ... | 2004 | 15260347 |
| inoculation of a submerged filter for biological denitrification of nitrate polluted groundwater: a comparative study. | activated sludge from a wastewater treatment plant and pure culture of hydrogenophaga pseudoflava were utilized for the development of a denitrifying biofilm in a submerged filter in order to remove nitrate from polluted groundwater. nitrate removal efficiency, nitrite accumulation, turbidity, cod and faecal indicators persistence in the treated water were determined at different superficial hydraulic loading (10, 20 and 30 m(3)/m(2) d) and superficial nitrate loading rates (1, 2, 3, 6 and 9 kg ... | 2005 | 15629573 |
| hydrogenophaga defluvii sp. nov. and hydrogenophaga atypica sp. nov., isolated from activated sludge. | two gram-negative, oxidase-positive rods (strains bsb 9.5t and bsb 41.8t) isolated from wastewater were studied using a polyphasic approach. 16s rrna gene sequence comparisons demonstrated that both strains cluster phylogenetically within the family comamonadaceae: the two strains shared 99.9 % 16s rrna gene sequence similarity and were most closely related to the type strains of hydrogenophaga palleronii (98.5 %) and hydrogenophaga taeniospiralis (98.0 %). the fatty acid patterns and substrate- ... | 2005 | 15653898 |
| malikia granosa gen. nov., sp. nov., a novel polyhydroxyalkanoate- and polyphosphate-accumulating bacterium isolated from activated sludge, and reclassification of pseudomonas spinosa as malikia spinosa comb. nov. | a gram-negative, motile, rod-shaped bacterium, designated strain p1(t), was isolated from activated sludge of a municipal wastewater treatment plant. phylogenetic analysis of its 16s rrna gene sequence placed the novel isolate among representatives of the family comamonadaceae. the closest relatives in reconstructed phylogenetic trees were pseudomonas spinosa, macromonas bipunctata and hydrogenophaga species. strain p1(t) was not able to grow anaerobically or autotrophically, reduced nitrate to ... | 2005 | 15774634 |
| diversity of bacteria growing in natural mineral water after bottling. | bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. using the full-cycle rrna approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bottling. seven 16s rrna gene libraries, comprising 108 clones in total, were constructed from water samples taken at various days after bottling and from two different bottle sizes. se ... | 2005 | 16000770 |
| autohydrogenotrophic denitrifying microbial community in a glass beads biofilm reactor. | a glass bead biofilm reactor was operated using h2 as an electron donor to remove nitrate at 150 mg no3-n l(-1) to below detection level. the microbial community in the glass beads biofilm reactor was investigated by using denaturing gradient gel electrophoresis (dgge) and phylogenetic analysis. in dgge analysis of the biofilm, five bands were dominant and indicated the presence of eight beta-proteobacteria, one gamma-proteobacteria and twelve clostridia. an unculturable hydrogenophaga sp., whic ... | 2005 | 16091891 |
| identification of bacteria potentially responsible for oxic and anoxic sulfide oxidation in biofilters of a recirculating mariculture system. | bacteria presumably involved in oxygen- or nitrate-dependent sulfide oxidation in the biofilters of a recirculating marine aquaculture system were identified using a new application of reverse transcription-pcr denaturing gradient gel electrophoresis (dgge) analysis termed differential-transcription (dt)-dgge. biofilter samples were incubated in various concentrations of sulfide or thiosulfate (0 to 5 mm) with either oxygen or nitrate as the sole electron acceptor. before and after short-term in ... | 2005 | 16204531 |
| molecular analysis of microbial population transition associated with the start of denitrification in a wastewater treatment process. | the objective of this study is to determine the bacteria playing an important role in denitrification by monitoring the molecular dynamics accompanying the start of denitrification. | 2005 | 16238747 |
| changes in bacterial community structure correlate with initial operating conditions of a field-scale denitrifying fluidized bed reactor. | high levels of nitrate are present in groundwater migrating from the former waste disposal ponds at the y-12 national security complex in oak ridge, tn. a field-scale denitrifying fluidized bed reactor (fbr) was designed, constructed, and operated with ethanol as an electron donor for the removal of nitrate. after inoculation, biofilms developed on the granular activated carbon particles. changes in the bacterial community of the fbr were evaluated with clone libraries (n = 500 partial sequences ... | 2006 | 16292532 |
| community and cultivation analysis of arsenite oxidizing biofilms at hot creek. | at hot creek in california, geothermally derived arsenite is rapidly oxidized to arsenate. this process is mediated by microorganisms colonizing the surfaces of submerged aquatic macrophytes in the creek. here we describe a multifaceted approach to characterizing this biofilm community and its activity. molecular techniques were used to describe the community as a function of 16s-rrna gene diversity. cultivation-based strategies were used to enumerate and isolate three novel arsenite oxidizers, ... | 2006 | 16343321 |
| isolation and characterization of a biphenyl-utilizing psychrotrophic bacterium, hydrogenophaga taeniospiralis ia3-a, that cometabolize dichlorobiphenyls and polychlorinated biphenyl congeners in aroclor 1221. | a psychrotrophic bacterium isolated from polychlorinated biphenyls (pcbs)-contaminated soil grew on biphenyl as sole carbon and energy source, and actively cometabolized pcbs at low temperature. analysis of cellular fatty acids indicate that the bacterium is most closely related to hydrogenophaga taeniospiralis. resting cells incubated with 10 ppm of aroclor 1221 at 5 or 30 degrees c for 48 h removed all mono-, most di-, and several trichlorobiphenyls. at 5 degrees c, removal of mcbs (monochloro ... | 2006 | 16598832 |
| cometabolic degradation of polychlorinated biphenyls at low temperature by psychrotolerant bacterium hydrogenophaga sp. ia3-a. | a biphenyl-utilizing bacterium isolated from polychlorinated biphenyls (pcbs)-contaminated soils grew on tryptic soy at temperatures between 4 and 40 degrees c. the gram-negative rod bacterium formed yellow colonies on nutrient agar and it denitrified nitrate to nitrogen. analysis of cellular fatty acids showed that it was most closely related to hydrogenophaga taeniospiralis. at 5 degrees c, biphenyl-grown cells cometabolically degraded di- and trichlorinated isomers of pcbs in 10 ppm of aroclo ... | 2006 | 16775787 |
| polyphosphate accumulation among denitrifying bacteria in activated sludge. | bacterial polyphosphate accumulation and denitrification are important processes in biological removal of nutrients from wastewater. it has been suggested that phosphorus accumulators are able to denitrify. however, the bacteria known as the most important phosphorus accumulators, belonging to the genus acinetobacter are generally not known to denitrify. to clarify how commonly both physiological traits are present in the same organism, we screened 165 isolates from activated sludge and wastewat ... | 1995 | 16887523 |
| heterogeneous aerobic benzene-degrading communities in oxygen-depleted groundwaters. | a sandstone aquifer beneath a petrochemicals plant (siren site, uk) is heterogeneously contaminated with benzene and oxygen-depleted. despite low redox potentials in three of the most contaminated groundwaters (benzene concentrations from 17.8 to 294 mg l(-1)), we observed aerobic benzene degradation in microcosms, indicating the presence in situ of a latent community of obligate aerobic microorganisms or an active community of facultative aerobes responding rapidly to oxygen ingress. moreover, ... | 2006 | 17064267 |
| characterization of the genes encoding the 3-carboxy-cis,cis-muconate-lactonizing enzymes from the 4-sulfocatechol degradative pathways of hydrogenophaga intermedia s1 and agrobacterium radiobacter s2. | hydrogenophaga intermedia strain s1 and agrobacterium radiobacter strain s2 form a mixed bacterial culture which degrades sulfanilate (4-aminobenzenesulfonate) by a novel variation of the beta-ketoadipate pathway via 4-sulfocatechol and 3-sulfomuconate. it was previously proposed that the further metabolism of 3-sulfomuconate is catalysed by modified 3-carboxy-cis,cis-muconate-lactonizing enzymes (cmles) and that these 'type 2' enzymes were different from the conventional cmles ('type 1') from t ... | 2006 | 17074892 |
| potential of various archae- and eubacterial strains as industrial polyhydroxyalkanoate producers from whey. | three different microbial wild-type strains are compared with respect to their potential as industrial scale polyhydroxyalkanoate (pha) producers from the feed stock whey lactose. the halophilic archaeon haloferax mediterranei as well as two eubacterial strains (pseudomonas hydrogenovora and hydrogenophaga pseudoflava) are investigated. h. mediterranei accumulated 50 wt.-% of poly-3-(hydroxybutyrate-co-8%-hydroxyvalerate) from hydrolyzed whey without addition of 3-hydroxyvalerate (3hv) precursor ... | 2007 | 17295410 |
| temperature-dependent biotransformation of 2,4'-dichlorobiphenyl by psychrotolerant hydrogenophaga strain ia3-a: higher temperatures prevent excess accumulation of problematic meta-cleavage products. | the present work investigates the possibility that temperature could regulate the pattern of transformation of 2,4'-chlorobiphenyl (2,4'-cb) by psychrotolerant hydrogenophaga sp. ia3-a. | 2007 | 17397486 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| biodegradation of polychlorinated biphenyls in aroclor 1232 and production of metabolites from 2,4,4'-trichlorobiphenyl at low temperature by psychrotolerant hydrogenophaga sp. strain ia3-a. | to determine the extent and pattern of degradation of polychlorinated biphenyls (pcbs) in aroclor 1232 at 5 degrees c by a psychrotolerant bacterium, and to confirm the formation of intermediates of pcb metabolism at low temperature using 2,4,4'-trichlorobiphenyl (2,4,4'-tcb). | 2007 | 17448167 |
| hydrogenophaga caeni sp. nov., isolated from activated sludge. | a gram-negative bacterium, designated strain emb71(t), was isolated from activated sludge used for enhanced biological phosphorus removal in a sequencing batch reactor. the cells of the isolate were facultatively aerobic, motile rods with single polar flagella. growth was observed to occur at 15-35 degrees c (optimally at 30 degrees c) and at ph 6.0-9.0 (optimally at ph 7.0-8.0). the predominant fatty acids of strain emb71(t) were c(16 : 0) and summed feature 3 (c(16 : 1)omega7c and/or iso-c(15 ... | 2007 | 17473270 |
| aerobic biodegradation of tert-butyl alcohol (tba) by psychro- and thermo-tolerant cultures derived from granular activated carbon (gac). | tert-butyl alcohol (tba) is a metabolite of methyl tert-butyl ether and is itself possibly a fuel oxygenate. the goals of this study were to enrich and characterize tba-degrading micro-organism(s) from a granular activated carbon (gac) unit currently treating tba. the results reported herein describe the first aerobic, tba-degrading cultures derived from gac. strains kr1 and yz1 were enriched from a gac sample in a bicarbonate-buffered freshwater medium. tba was degraded to 10% of the initial co ... | 2008 | 17541707 |
| aerobic mtbe biodegradation in the presence of btex by two consortia under batch and semi-batch conditions. | this study explores the effect of microbial consortium composition and reactor configuration on methyl tert-butyl ether (mtbe) biodegradation in the presence of benzene, toluene, ethylbenzene and p-xylenes(btex). mtbe biodegradation was monitored in the presence and absence of btex in duplicate batch reactors inoculated with distinct enrichment cultures: mtbe only (mo-originally enriched on mtbe) and/or mtbe btex (mb-originally enriched on mtbe and btex). the mo culture was also applied in a sem ... | 2008 | 17562189 |
| 4-sulfomuconolactone hydrolases from hydrogenophaga intermedia s1 and agrobacterium radiobacter s2. | the 4-carboxymethylen-4-sulfo-but-2-en-olide (4-sulfomuconolactone) hydrolases from hydrogenophaga intermedia strain s1 and agrobacterium radiobacter strain s2 are part of a modified protocatechuate pathway responsible for the degradation of 4-sulfocatechol. in both strains, the hydrolase-encoding genes occur downstream of those encoding the enzymes that catalyze the lactonization of 3-sulfomuconate. the deduced amino acid sequences of the 4-sulfomuconolactone hydrolases demonstrated the highest ... | 2007 | 17660282 |
| characterization of novel linuron-mineralizing bacterial consortia enriched from long-term linuron-treated agricultural soils. | linuron-mineralizing cultures were enriched from two linuron-treated agricultural soils in the presence and absence of a solid support. the cultures contained linuron-degrading bacteria, which coexisted with bacteria degrading either 3,4-dichloroaniline (3,4-dca) or n,o-dimethylhydroxylamine (n,o-dmha), two common metabolites in the linuron degradation pathway. for one soil, the presence of a solid support enriched for linuron-degrading strains phylogenetically related to but different from thos ... | 2007 | 17991021 |
| isolation and characterization of a new facultatively autotrophic hydrogen-oxidizing betaproteobacterium, hydrogenophaga sp. ah-24. | a hydrogen-oxidizing bacterium strain ah-24 was isolated, which was classified in the genus hydrogenophaga, based on the 16s rrna gene sequence. the isolate possessed a typical yellow pigment of hydrogenophaga species. its closest relative was hydrogenophaga pseudoflava, but the assimilation profile of sugar compounds resembled that of no species of hydrogenophaga. the optimum temperature and ph for autotrophic growth were, respectively, 33-35 degrees c and 7.0. most hydrogenase activity (benzyl ... | 2008 | 18031533 |
| hydrogenophaga bisanensis sp. nov., isolated from wastewater of a textile dye works. | a gram-negative, non-spore-forming, rod-shaped, hydrogenophaga-like bacterial strain, k102(t), was isolated from wastewater collected from a textile dye works in korea and subjected to a polyphasic taxonomic study. strain k102(t) grew optimally at ph 7.0-8.0 and 30-37 degrees c in the presence of 0.5 % (w/v) nacl. it contained q-8 as the predominant ubiquinone and c(16 : 0), c(16 : 1)omega7c and/or iso-c(15 : 0) 2-oh and c(18 : 1)omega7c as the major fatty acids. the dna g+c content was 64.8 mol ... | 2008 | 18218937 |
| isolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer. | to isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (btex) from the siren aquifer, uk, and to test their effective ph range and ability to degrade tex. | 2008 | 18544140 |
| phylogenetic and metabolic bacterial diversity of phragmites australis periphyton communities in two hungarian soda ponds. | bacterial diversity of reed (phragmites australis) periphyton communities of kelemen-szék and nagy-vadas (two hungarian soda ponds) was investigated using molecular cloning and cultivation-based techniques. the majority of the 80 kelemen-szék and 72 nagy-vadas bacterial isolates proved to be moderately halophilic and alkaliphilic. a great proportion of the isolates showed phosphatase and urease activity, utilized aesculin, citrate and certain biopolymers (e.g., gelatine and tween 80). partial 16 ... | 2008 | 18679563 |
| novel autotrophic arsenite-oxidizing bacteria isolated from soil and sediments. | arsenic oxidation is recognized as being mediated by both heterotrophic and chemoautotrophic microorganisms. enrichment cultures were established to determine whether chemoautotrophic microorganisms capable of oxidizing arsenite as(iii) to arsenate as(v) are present in selected contaminated but nonextreme environments. three new organisms, designated as strains ol-1, s-1 and cl-3, were isolated and found to oxidize 10 mm arsenite to arsenate under aerobic conditions using co2-bicarbonate (co2/hc ... | 2008 | 18717738 |
| bacterial succession within an ephemeral hypereutrophic mojave desert playa lake. | ephemerally wet playas are conspicuous features of arid landscapes worldwide; however, they have not been well studied as habitats for microorganisms. we tracked the geochemistry and microbial community in silver lake playa, california, over one flooding/desiccation cycle following the unusually wet winter of 2004-2005. over the course of the study, total dissolved solids increased by approximately 10-fold and ph increased by nearly one unit. as the lake contracted and temperatures increased ove ... | 2009 | 18758846 |
| purification and biochemical characterization of a membrane-bound [nife]-hydrogenase from a hydrogen-oxidizing, lithotrophic bacterium, hydrogenophaga sp. ah-24. | membrane-bound [nife]-hydrogenase from hydrogenophaga sp. ah-24 was purified to homogeneity. the molecular weight was estimated as 100+/-10 kda, consisting of two different subunits (62 and 37 kda). the optimal ph values for h(2) oxidation and evolution were 8.0 and 4.0, respectively, and the activity ratio (h(2) oxidation/h(2) evolution) was 1.61 x 10(2) at ph 7.0. the optimal temperature was 75 degrees c. the enzyme was quite stable under air atmosphere (the half-life of activity was c. 48 h a ... | 2009 | 19025569 |
| mixed aerobic and anaerobic microbial communities in benzene-contaminated groundwater. | to investigate the factors affecting benzene biodegradation and microbial community composition in a contaminated aquifer. | 2009 | 19120616 |
| carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a canadian mill. | forty-one bacterial strains isolated from the headbox water of two machines in a canadian paper mill were associated with the genera asticcacaulis, acidovorax, bacillus, exiguobacterium, hydrogenophaga, pseudomonas, pseudoxanthomonas, staphylococcus, stenotrophomonas based on the sequence of their 16s rrna genes. the metabolic profile of these strains were determined using biolog ecoplate, and the bacteria were divided into four metabolic groups. metabolic profiles of the bacterial communities c ... | 2009 | 19137341 |
| [variation of bacterial community composition in the outbreak and decline of microcystis spp. bloom in lake xuanwu]. | water samples were collected in the outbreak and decline of microcystis spp. bloom in three different lake areas of lake xuanwu for studying the bacterial community composition during the bloom to provide clew for understanding the dynamics of water bloom. traditional microbiological methods were used for culturable bacterial analysis, while the amplified products of variable v3 region of bacterial 16s rdna were used for bacterial community dna finger print by using denaturing gradient gel elect ... | 2008 | 19143401 |
| characterization of geochemical constituents and bacterial populations associated with as mobilization in deep and shallow tube wells in bangladesh. | while millions of people drink arsenic-contaminated tube well water across bangladesh, there is no recent scientific explanation which is able to either comprehensively explain arsenic mobilization or to predict the spatial distribution of affected wells. rather, mitigation strategies have focused on the sinking of deep tube wells into the currently arsenic-free pleistocene aquifer. in this study, bangladesh shallow tube wells identified as contaminated and uncontaminated, as well as deep tube w ... | 2009 | 19215956 |
| bacterial communities of tyre monofill sites: growth on tyre shreds and leachate. | to investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria. | 2009 | 19239530 |
| polaromonas and hydrogenophaga species are the predominant bacteria cultured from granular activated carbon filters in water treatment. | identification of the predominating cultivable bacteria in granular activated carbon (gac) filters used in a variety of water treatment plants for selecting representative strains to study the role of bacteria in the removal of dissolved organic matter. | 2009 | 19457026 |
| autohydrogenotrophic denitrification of drinking water using a polyvinyl chloride hollow fiber membrane biofilm reactor. | a hollow fiber membrane biofilm reactor (mbfr) using polyvinyl chloride (pvc) hollow fiber was evaluated in removing nitrate form contaminated drinking water. during a 279-day operation period, the denitrification rate increased gradually with the increase of influent nitrate loading. the denitrification rate reached a maximum value of 414.72 g n/m(3)d (1.50 g n/m(2)d) at an influent no(3)(-)-n concentration of 10mg/l and a hydraulic residence time of 37.5 min, and the influent nitrate was compl ... | 2009 | 19473764 |
| biphenyl-metabolizing bacteria in the rhizosphere of horseradish and bulk soil contaminated by polychlorinated biphenyls as revealed by stable isotope probing. | dna-based stable isotope probing in combination with terminal restriction fragment length polymorphism was used in order to identify members of the microbial community that metabolize biphenyl in the rhizosphere of horseradish (armoracia rusticana) cultivated in soil contaminated with polychlorinated biphenyls (pcbs) compared to members of the microbial community in initial, uncultivated bulk soil. on the basis of early and recurrent detection of their 16s rrna genes in clone libraries construct ... | 2009 | 19700551 |
| use of hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters. | filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. using hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. we show that con ... | 2010 | 19966023 |
| composition and dynamics of sulfate-reducing bacteria during the waterflooding process in the oil field application. | the composition and dynamics of sulfate-reducing bacteria (srb) during the waterflooding process of daqing oilfield were investigated in this study. pcr-dgge analysis indicated that the microbial communities were significantly different in each treatment unit, and the dominant members were mainly close to clostridium sp., thauera sp., hydrogenophaga sp., pseudomonas sp., eubacterium sp. and arcobacter sp. however, the members belonging to srb were relatively few and mainly consisted of desulfovi ... | 2010 | 20005702 |
| [characteristics and diversity of a microbial community decomposing wheat straw under aerobic conditions]. | a microbial community with the stable ability of effectively decomposing wheat stalks was constructed at room temperature under aerobic conditions by the method of restricted cultivation. the degradation ability of the microbial community shows that when performed in 100 ml improved cmc liquid medium, the depth and diamerer of the fluid level were 2 cm and 9 cm, it kept higher degradation efficiency with capability of decomposing straws of 66.1% during the initial six days. by the tenth day of d ... | 2010 | 20329547 |
| elucidating mtbe degradation in a mixed consortium using a multidisciplinary approach. | the structure and function of a microbial community capable of biodegrading methyl-tert-butyl ether (mtbe) was characterized using compound-specific stable isotope analysis (csia), clone libraries and stable isotope probing of proteins (protein-sip). the enrichment culture (us3-m), which originated from a gasoline-impacted site in the united states, has been enriched on mtbe as the sole carbon source. the slope of isotopic enrichment factors (epsilon(c) of -2.29+/-0.03 per thousand; epsilon(h) o ... | 2010 | 20491917 |
| postbiostimulation microbial community structure changes that control the reoxidation of uranium. | this study evaluated the influence of changes in the microbial community structure on reoxidation of reduced uranium during a postbiostimulation period. effluent groundwater from acetate-stimulated sediment flow-through columns was analyzed over 60 days after acetate amendment was discontinued. only a small reoxidation of iron or uranium (17%) occurred in the presence of 1-2 mg l(-1) o(2) influent groundwater for the 2-month period. most uranium reoxidation occurred during the first 2 weeks afte ... | 2010 | 20707815 |
| biodegradation of 4-aminobenzenesulfonate by ralstonia sp. pba and hydrogenophaga sp. pbc isolated from textile wastewater treatment plant. | a co-culture consisting of hydrogenophaga sp. pbc and ralstonia sp. pba, isolated from textile wastewater treatment plant could tolerate up to 100 mm 4-aminobenzenesulfonate (4-abs) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. the biodegradation of 4-abs resulted in the release of nitrogen and sulfur in the form of ammonium and sulfate respectively. ninety-eight percent removal of chemical oxygen demand attributed to 20 mm of 4-abs in cell-free supernatant c ... | 2010 | 21094980 |
| hydrogenophaga temperata sp. nov., a betaproteobacterium isolated from compost in korea. | a gram-negative, rod-shaped, non-spore-forming and motile bacterial strain tr7-01(t) was isolated from a compost soil in south korea and subjected to a polyphasic taxonomic study. phylogenetic analysis based on 16s rrna gene sequences showed that strain tr7-01(t) belonged to the genus hydrogenophaga within the class betaproteobacteria. strain tr7-01(t) exhibited 16s rrna gene sequence similarity values of 95.0-98.3% to members of the genus hydrogenophaga: hydrogenophaga bisanensis dsm12412(t) (9 ... | 2010 | 21282897 |
| identification of tertiary butyl alcohol (tba)-utilizing organisms in biogac reactors using 13c-dna stable isotope probing. | biodegradation of the gasoline oxygenates methyl tertiary-butyl ether (mtbe) and ethyl tertiary-butyl ether (etbe) can cause tertiary butyl alcohol (tba) to accumulate in gasoline-impacted environments. one remediation option for tba-contaminated groundwater involves oxygenated granulated activated carbon (gac) reactors that have been self-inoculated by indigenous tba-degrading microorganisms in ground water extracted from contaminated aquifers. identification of these organisms is important for ... | 2011 | 21286787 |
| identification of genes involved in the 4-aminobenzenesulfonate degradation pathway of hydrogenophaga sp. pbc via transposon mutagenesis. | genes involved in the 4-aminobenzenesulfonate (4-abs) degradation pathway of hydrogenophaga sp. pbc were identified using transposon mutagenesis. the screening of 10,000 mutants for incomplete 4-abs biotransformation identified four mutants with single transposon insertion. genes with insertions that impaired the ability to utilize 4-abs for growth included (1) 4-sulfocatechol 1,2-dioxygenase ß-subunit (pcah2) and 3-sulfomuconate cycloisomerase involved in the modified ß-ketoadipate pathway; (2) ... | 2011 | 21323982 |
| food quality of heterotrophic bacteria for daphnia magna: evidence for a limitation by sterols. | the quality of heterotrophic bacteria as food for metazoan grazers has been investigated poorly. we conducted growth experiments with juvenile daphnia magna feeding on different strains of heterotrophic bacteria that represent typical pelagic bacteria of five phylogenetically distinct groups. the bacterial food suspensions were supplemented with cholesterol and/or the polyunsaturated fatty acid eicosapentaenoic acid (epa), two essential nutrients that are either absent or scarcely represented in ... | 2011 | 21426363 |
| simultaneous removal of hydrogen sulfide and toluene in a bioreactor: performance and characteristics of microbial community. | we investigated the correlation between performance and the bacteria community composition by h2s and toluene co-treatment. operation of the bioreactor was divided into four stages, in which the inlet concentration of toluene and h2s were gradually increased. in stage i, toluene was the sole target compound with an average removal efficiency of 86.49%. after adding h2s in stage ii, removal efficiency of toluene decreased immediately and recovered gradually to 85.96%. when the inlet concentration ... | 2011 | 21520802 |
| impact of biochar application to soil on the root-associated bacterial community structure of fully developed greenhouse pepper plants. | adding biochar to soil has environmental and agricultural potential due to its long-term carbon sequestration capacity and its ability to improve crop productivity. recent studies have demonstrated that soil-applied biochar promotes systemic resistance of plants to several prominent foliar pathogens. one potential mechanism for this phenomenon is root-associated microbial elicitors whose presence is somehow augmented in the biochar-amended soils. the objective of this study was to assess the eff ... | 2011 | 21622786 |
| degradation of a benzene-toluene mixture by hydrocarbon-adapted bacterial communities. | we examined the rate of degradation of a benzene-toluene mixture in aerobic microcosms prepared with samples of an aquifer that lies below a petrochemical plant (siren, uk). five samples exposed to different concentrations of benzene (from 0.6 to 317 mg l(-1)) were used. fast degradation (approx. 1-6 mg l(-1) day(-1)) of both contaminants was observed in all groundwater samples and complete degradation was recorded by the seventh day except for one sample. we also identified the microbial commun ... | 2011 | 21949494 |
| interactions between perchlorate and nitrate reductions in the biofilm of a hydrogen-based membrane biofilm reactor. | we studied the microbial functional and structural interactions between nitrate (no(3)(-)) and perchlorate (clo(4)(-)) reductions in the hydrogen (h(2))-based membrane biofilm reactor (mbfr). when h(2) was not limiting, clo(4)(-) and no(3)(-) reductions were complete, and the mbfr's biofilm was composed mainly of bacteria from the ε- and β-proteobacteria classes, with autotrophic genera sulfuricurvum, hydrogenophaga, and dechloromonas dominating the biofilm. based on functional-gene and pyrosequ ... | 2011 | 22017212 |
| evaluation of microbial biofilm communities from an alberta oil sands tailings pond. | bitumen extraction from the oil sands of alberta has resulted in millions of cubic meters of waste stored on-site in tailings ponds. unique microbial ecology is expected in these ponds, which may be key to their bioremediation potential. we considered that direct culturing of microbes from a tailings sample as biofilms could lead to the recovery of microbial communities that provide good representation of the ecology of the tailings. culturing of mixed species biofilms in vitro using the calgary ... | 2011 | 22029695 |
| Use of specific gene analysis to assess the effectiveness of surfactant-enhanced trichloroethylene cometabolism. | The objective of this study was to evaluate the effectiveness of in situ bioremediation of trichloroethylene (TCE)-contaminated groundwater using specific gene analyses under the following conditions: (1) pretreatment with biodegradable surfactants [Simple Green™ (SG) and soya lecithin (SL)] to enhance TCE desorption and dissolution, and (2) supplementation with SG, SL, and cane molasses as primary substrates to enhance the aerobic cometabolism of TCE. Polymerase chain reaction (PCR), denaturing ... | 2011 | 22071259 |
| betaproteobacteria dominance and diversity shifts in the bacterial community of a pah-contaminated soil exposed to phenanthrene. | in this study, the pah-degrading bacteria of a constructed wetland collecting road runoff has been studied through dna stable isotope probing. microcosms were spiked with (13)c-phenanthrene at 34 or 337 ppm, and bacterial diversity was monitored over a 14-day period. at 337 ppm, pah degraders became dominated after 5 days by betaproteobacteria, including novel acidovorax, rhodoferax and hydrogenophaga members, and unknown bacteria related to rhodocyclaceae. the prevalence of betaproteobacteria w ... | 2012 | 22243884 |