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staphylococcus pasteuri-specific oligonucleotide probes derived from a random amplified dna fragment.a rapid polymerase chain reaction method was developed to differentiate staphylococcus pasteuri from other staphylococcal species, especially the phenotypically similar s. warneri. the oligonucleotide probes used as primers were designed from the sequence of a s. pasteuri random amplified polymorphic dna fragment.19957590156
staphylococcus pasteuri sp. nov., isolated from human, animal, and food specimens.a new novobiocin-susceptible species of the genus staphylococcus, staphylococcus pasteuri, is described on the basis of the results of a study of seven strains isolated from human, animal, and food specimens. dna relatedness experiments (s1 nuclease method) showed that these strains form a homogeneous genomic species related at dna homology levels of 2 to 13% to 27 type strains representing known staphylococcus species. the use of a method based on rrna gene restriction site polymorphism provide ...19938098615
microorganisms cultured from stratospheric air samples obtained at 41 km.samples of air removed from the stratosphere, at an altitude of 41 km, were previously found to contain viable, but non-cultureable bacteria (cocci and rods). here, we describe experiments aimed at growing these, together with any other organisms, present in these samples. two bacteria (bacillus simplex and staphylococcus pasteuri) and a single fungus, engyodontium album (limber) de hoog were isolated from the samples. although the possibility of contamination can never be ruled out when space-d ...200312583913
identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated ribotyping.as routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ...200515715714
developing microbial spoilage population in vacuum-packaged charcoal-broiled european river lamprey (lampetra fluviatilis).microbiological and sensory changes in vacuum-packaged charcoal-broiled river lampreys from three lamprey processing plants were monitored as a function of time at 8 degrees c. the lampreys were examined every 7 days up to 8 weeks for aerobic plate count (apc) and lactic acid bacteria (lab). the highest mean apc and lab were 6.01 log cfu/g and 4.86 log cfu/g, respectively. only 6 out of 15 lots reached an apc value of 7.0 log cfu/g during storage. the sensory scores remained at the baseline leve ...200415862876
determination of microbial volatile organic compounds from staphylococcus pasteuri against tuber borchii using solid-phase microextraction and gas chromatography/ion trap mass spectrometry.the mycelium of tuber borchii vittad., a commercial truffle species, is used as a model system for in vitro ectomycorrhizal synthesis, infected seedling production and biotechnological applications. our fungal cultures were accidentally contaminated with a staphylococcus pasteuri strain, showing a strong antifungal activity against t. borchii mycelium. in order to identify the antifungal volatile agents produced by s. pasteuri, solid-phase microextraction (spme) with gas chromatography and mass ...200516259047
ecology and dynamics of coagulase-negative cocci isolated from naturally fermented italian sausages.coagulase-negative cocci (cnc) ecology in naturally fermented sausages from friuli venezia giulia region, in the north east of italy, was investigated. a total of 617 cnc strains, isolated from three different plants during the fermentation process, were identified by traditional methods (biochemical tests) and molecular methods based on species specific pcr, pcr-denaturing gradient gel electrophoresis (dgge) and sequencing of the v3 region of the 16s rrna gene. the identification, by using bioc ...200616337767
the smr gene resides on a novel plasmid psp187 identified in a staphylococcus pasteuri isolate recovered from unpasteurized milk.this work describes a novel plasmid psp187 (5550 bp) carrying the small multidrug resistance determinant smr encoding resistance to quaternary ammonium compounds (qacs). psp187 was identified in a staphylococcus pasteuri isolate recovered from bulk milk in a dairy cattle herd in norway. sequence analysis revealed 6 putative orfs in addition to the smr gene within a cassette with identical genetic organization to that found in the psk41-like staphylococcus aureus plasmid ptz22. a protein homology ...200717074390
antibiotic resistance genes and identification of staphylococci collected from the production chain of swine meat commodities.staphylococci harbouring antibiotic resistance (ar) genes may represent a hazard for human health and, as other resistant food-related bacteria, they contribute to the spread of ar. in this study, we isolated resistant staphylococci from an entire swine production chain and investigated the occurrence of 11 genes [aac(6')ie-aph(2'')ia, blaz, meca, vana, vanb, erma, ermb, ermc, tet(m), tet(o) and tet(k)] encoding resistance to some antibiotics largely used in clinical practice. the 66 resistant s ...200817993395
cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments.this study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in johannesburg, south africa. samples of each cleaning or handling tool were prepared for aerobic (apc), coliform (cc), escherichia coli (ec), bacillus cereus (bcc), and staphylococcus aureus (sac) counts, as ...200718095448
contamination of a donated platelet unit by staphylococcus pasteuri. 200819027171
antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water.this study reports the antibiotic resistance patterns of coagulase negative staphylococci (cns) isolated from a drinking water treatment plant (wtp), a drinking water distribution network, responsible for supplying water to the consumers (wdn), and a wastewater treatment plant (wwtp), responsible for receiving and treating domestic residual effluents. genotyping and the 16s rrna gene sequence analysis demonstrated a higher diversity of species both in the wtp (6 species/19 isolates) and wwtp (12 ...200919324394
epidemiology, pathogenicity and emerging resistances in staphylococcus pasteuri: from mammals and lampreys, to man.staphylococcus pasteuri is a coagulase-negative, gram positive organism which is emerging as an agent of nosocomial infections and a blood derivatives contaminant, though its role in causing human disease mostly remains controversial. despite the paucity of isolates recovered, this bacterium has recently appeared to express resistance against several classes of antibiotic compounds, such as methicillin/oxacillin, macrolides, lincosamides, streptogramins, tetracyclines, chloramphenicol, streptomy ...200919519547
staphylococcus pasteuri bacteraemia in a patient with leukaemia. 200919542075
mixed-substrate (glycerol tributyrate and fibrin) zymography for simultaneous detection of lipolytic and proteolytic enzymes on a single gel.a new zymography method for simultaneous detection of two different enzymatic activities (lipolytic and proteolytic) using a single sds-containing or native-conformation gel and a mixed-substrate (glycerol tributyrate and fibrin) (ms)(1) gel was developed. after routine electrophoresis, sds in the gel was removed by treatment with triton x-100. gel proteins were electrotransferred to the ms gel. to visualize lipolytic activity, the ms gel was incubated at 37 degrees c (for 6 or 24 h) until clear ...200919544489
meticillin-heteroresistant staphylococcus pasteuri from an apheresis platelet product. 200919608691
diversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance.the objective of this study was to determine the distribution of staphylococcal cassette chromosome mec (sccmec) elements in meticillin-resistant coagulase-negative staphylococci (mr-cons) isolated from a tertiary-care hospital in mexico and to examine the relationship to drug resistance. fifty selected mr-cons isolates collected from catheters (n=15), blood (n=15), bone (n=9), bronchial lavage (n=2) and urine (n=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion ...201020007762
safety-related properties of staphylococci isolated from food and food environments.to test some safety-related properties within 321 staphylococci strains isolated from food and food environments.201021143714
assessment and characterization of heavy metal resistance in palk bay sediment bacteria.the present study aimed at characterizing the heavy metal resistance and assessing the resistance pattern to multiple heavy metals (300 mmol l?¹) by palk bay sediment bacteria. from 46 isolates, 24 isolates showed resistance to more than eight heavy metals. among the 24 isolates s8-06 (bacillus arsenicus), s8-10 (bacillus pumilus), s8-14 (b. arsenicus), s6-01 (bacillus indicus), s6-04 (bacillus clausii), ss-06 (planococcus maritimus) and ss-08 (staphylococcus pasteuri) exhibited high resistance ...201121377723
species and staphylococcal cassette chromosome mec (sccmec) diversity among methicillin-resistant non-staphylococcus aureus staphylococci isolated from pigs.while methicillin-resistant staphylococcus aureus (mrsa) st398 is known to be widespread in pig farms, few studies have investigated the species diversity and sccmec types of methicillin-resistant non-s. aureus staphylococci (mrnas) residing in the nose of pigs. we examined nasal swab samples of 200 pigs originating from 10 belgian pig farms previously found positive for mrsa st398. suspected staphylococcal isolates were subjected to a 16s rrna-meca-nuc pcr. confirmed mrnas were genotypically id ...201222333287
isolation of mammalian pathogenic bacteria using silkworms.we developed a method to predict bacterial pathogenicity against mammals by measuring bacterial virulence in silkworms at 37°c, human body temperature. one hundred and twenty-two strains of bacteria were isolated from the intestines of fish and shellfish and tested for their virulence against silkworms. overnight cultures of 50 strains killed at least 50% of the silkworms when injected into the hemolymph. of 10 strains that showed the most potent pathogenicity against silkworms, 8 also killed mi ...201122466142
isolation of rare coagulase-negative isolates in immunocompromised patients: staphylococcus gallinarum, staphylococcus pettenkoferi and staphylococcus pasteuri.herein, we describe the isolation of staphylococcus pasteuri, staphylococcus pettenkoferi and staphylococcus gallinarum and summarize the clinical characteristics of five patients. cases were identified over a 2-year surveillance period that identified the respective strains using microbiologic and molecular methods. these data suggest that rare coagulase-negative staphylococcal infections may be under-diagnosed due to difficulties associated with routine clinical laboratory diagnostic methods.201222585615
biogenic amine content, histamine-forming bacteria, and adulteration of pork in tuna sausage products.twenty-five tuna sausage products were purchased from retail markets in taiwan. the rates of occurrence of biogenic amines, histamine-forming bacteria, and adulteration by pork and poultry were determined. the average content of various biogenic amines in all tested samples was less than 2.0 mg/100 g (<0.05 to 1.85 mg/100 g). thirteen histamine-producing bacterial strains isolated from tested samples produced 12.1 to 1,261 ppm of histamine in trypticase soy broth supplemented with 1.0% l-histidi ...201223043830
technological and safety characteristics of staphylococcaceae isolated from spanish traditional dry-cured sausages.the aim of this study was to determine the technological properties (nitrate reductase, proteolytic and lipolytic activities; and the ability to grow at the temperature and ph values of fermenting sausage, and at high nacl concentrations) and safety characteristics (amino acid decarboxylase and enterotoxigenic activities) of 38 strains of staphylococcaceae (11 of staphylococcus epidermidis, 15 of staphylococcus equorum, 5 of staphylococcus pasteuri and 7 of staphylococcus saprophyticus) isolated ...201323122502
effects of bacteria on cadmium bioaccumulation in the cadmium hyperaccumulator plant beta vulgaris var. cicla l.to investigate the effects of two cadmium-tolerant bacteria, staphylococcus pasteuri (s. pasteuri x1) and agrobacterium tumefaciens (a. tumefaciens x2), on cadmium uptake by the cadmium hyperaccumulator plant beta vulgaris var. cicla l., a pot experiment with artificially contaminated soil was conducted. the results demonstrated that both cadmium-tolerant bacteria enhanced the dry weight of beta vulgaris var. cicla l. the total dry weights of plants in the control ck20, s. pasteuri x1 and a. tum ...201323488173
duodenal-mucosal bacteria associated with celiac disease in children.celiac disease (cd) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. this disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of cd. the aim of this study was to characterize the composition and diversity of the cultivable duodenal mucosa-associated bacteria of cd patients and control children. duodenal biopsy specimens from patients with active disease on a gluten-containing diet (n = 32), pat ...201323835180
three proposed new bacteriophage genera of staphylococcal phages: "3alikevirus", "77likevirus" and "phietalikevirus".to date, most members of the siphoviridae family of bacteriophages remain unclassified, including the 46 staphylococcal phages for which the complete genome sequences have been deposited in public databases. comparative nucleotide and protein sequence analysis, in addition to available data on phage morphology, allowed us to propose three new phage genera within the family siphoviridae: "3alikevirus", "77likevirus" and "phietalikevirus", which include related phages infecting staphylococcus aure ...201424022640
evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption.vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. bioremediation is an efficient process for the treatment of contaminated sites. efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. in this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. eighty isolates (45 from vineyard mollisol, 35 ...201124031606
characterization of methicillin-resistant coagulase-negative staphylococci (mrcons) in retail meat.this study was to understand the extent of methicillin-resistant coagulase-negative staphylococci (mrcons) serving as a meca reservoir in retail meat. mrcons were isolated from retail meat (beef, chicken, and turkey) in detroit and characterized by soda gene sequencing for species identification, staphylococcal cassette chromosome mec (sccmec) typing, and pulsed-field gel electrophoresis (pfge). unique mrcons isolates recovered from 25 meat samples were comprised of staphylococcus sciuri (n = 13 ...201424929717
american kestrel (falco spaverius) fledgling with severe bilateral periorbital swelling and infection with mycoplasma buteonis, avibacterium (pasteurella) gallinarum, and staphylococcus pasteuri.abstract: a female american kestrel (falco spaverius) fledgling was found on the ground with a suspected trauma to the right eye and open-mouth breathing. during the first 2 days of hospitalization, the bird developed severe bilateral periorbital cellulitis, blepharoedema, and sinusitis. the periocular tissues of the right globe were devitalized and communicated with a fistula at the commissure of the right side of the beak. the blepharoedema of the left eye was aspirated and yielded a dark colo ...201425115041
pretreatment of coconut mill effluent using celite-immobilized hydrolytic enzyme preparation from staphylococcus pasteuri and its impact on anaerobic digestion.biological treatment of oil and grease (o&g)-containing industrial effluents has long been a challenging issue. practically feasible avenues to bring down their o&g load and enhance treatability are desired. in one such endeavour, the partially purified lipase from staphylococcus pasteuri com-4a was immobilized on celite carrier and applied for the enzymatic hydrolysis of unsterilized coconut oil mill effluent. in batch hydrolysis experiments, optimum conditions of 1% (w/v) immobilized lipase be ...201526033963
bacterial profile and bone healing in rats receiving cancer therapeutic doses of bisphosphonates and corticosteroids: a pilot study.the microbial aetiology of bisphosphonate-related osteonecrosis of the jaw (bronj) remains undefined. this study investigated the oral microbiota and socket healing after zoledronic acid (za) and dexamethasone (dx) administration. fourteen rats assigned randomly to experimental (n=8) and control (n=6) groups were injected with za+dx or saline, respectively, for 3 weeks prior to and 9 weeks after the extraction of left first upper and lower molars. whole genomic dna probes of 38 bacterial species ...201626780925
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