Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a sequence-specific endonuclease (xmn i) from xanthomonas manihotis. | a type ii restriction endonuclease xmn i with a novel site specificity has been isolated from xanthomonas manihotis. xmn i does not cleave sv40 dna, but cleaves phi x174 dna into three fragments, which constitute 76.61%, 18.08% and 5.31% of the total length of 5386 base pairs, and cleaves pbr322 dna into two fragments of 55.71% and 44.29% of the entire 4362 base pairs. the nucleotide sequences around the cleavage sites made by xmn i are not exactly homologous, but they have a common sequence of ... | 1980 | 6258165 |
| purification and characterization of novel glycosidases from the bacterial genus xanthomonas. | enzymatic analysis of oligosaccharides using exoglycosidases has become a powerful tool for determining the sequence and structure of sugar chains. the principal limitation to these methods has been the lack of highly purified and well-characterized enzymes. using fluorescently labelled carbohydrate substrates and tlc, we have developed a method to identify glycosidases with novel specificities. this screening method led to the discovery that bacteria of the genus xanthomonas are a rich source o ... | 1995 | 7772864 |
| a novel beta-galactosidase gene isolated from the bacterium xanthomonas manihotis exhibits strong homology to several eukaryotic beta-galactosidases. | the gene encoding a beta-galactosidase from xanthomonas manihotis was cloned into escherichia coli. the gene resides on a 2.4 kb dna fragment which was isolated from a partial sau3a library in the cloning vector puc19 using 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (x-gal) as the selection. the enzyme produced by the clone has a specificity for beta 1-3- > beta 1-4-linked galactose. the nucleotide sequence of the gene was determined. the deduced protein sequence contained 597 amino aci ... | 1995 | 8563148 |
| the xmni restriction-modification system: cloning, expression, sequence organization and similarity between the r and m genes. | the xmnirm genes from xanthomonas manihotis 7as1 have been cloned and expressed in escherichia coli. the nucleotide (nt) sequences of both genes were determined. the xmni methyltransferase (mtase)-encoding gene is 1861 bp in length and codes for 620 amino acids (aa) (68660 da). the restriction endonuclease (enase)-encoding gene is 959 bp long and therefore codes for a 319-aa protein (35275 da). the two genes are aligned tail to tail and they overlap at their respective stop codons about 4 x 10(4 ... | 1996 | 8964488 |
| a novel arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases. | an arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a xanthomonas manihotis enzyme. phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade. the gene encodes a subunit of 52 kda, and the enzyme appears to be active as a dimer. the enzyme hydrolyzed substrates with either a beta-1,4 or a beta-1,3 linkage. | 1997 | 9139931 |
| cloning and characterization of the gene encoding a novel beta-galactosidase from bacillus circulans. | a novel beta-galactosidase (beta-gal) gene was cloned from bacillus circulans atcc 31382. the coding region was 1,758 bp and encoded a polypeptide of 586 amino acids with a deduced molecular mass of 66,888. active staining for beta-gal showed that b. circulans atcc 31382 produced three beta-gal isozymes. two of these were detected in biolacta n5 (daiwakasei co.), but the product of this novel gene corresponded to the one not contained in biolacta n5. the novel beta-gal showed the highest amino a ... | 1997 | 9301106 |
| extracellular polysaccharides from xanthomonas axonopodis pv. manihotis interact with cassava cell walls during pathogenesis. | the location of lipopolysaccharides produced by xanthomonas axonopodis pv. manihotis during pathogenesis on cassava (manihot esculenta) was determined by fluorescence and electron microscopy immunolabeling with monoclonal antibodies. during the early stages of infection, pathogen lipopolysaccharides were detected on the outer surface of the bacterial envelope and in areas of the plant middle lamellae in the vicinity of the pathogen. later in the infection process, lipopolysaccharide-specific ant ... | 1997 | 9304855 |
| aflp fingerprinting: an efficient technique for detecting genetic variation of xanthomonas axonopodis pv. manihotis. | xanthomonas axonopodis pv. manihotis (xam) is the causative agent of cassava bacterial blight (cbb), a worldwide disease that is particularly destructive in south america and africa. cbb is controlled essentially through the use of resistant varieties. to develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. until now, the genetic diversity of xam was characterized by rflp analyses using ribotyping, and plasmid and genomic xam p ... | 1999 | 10206688 |
| aflp assessment of genetic variability in cassava accessions (manihot esculenta) resistant and susceptible to the cassava bacterial blight (cbb). | cassava bacterial blight (cbb) is caused by xanthomonas axonopodis pv. manihotis (xam). resistance is found in manihot esculenta and, in addition, has been introgressed from a wild relative, m. glaziovii. the resistance is thought to be polygenic and additively inherited. ninety-three varieties of m. esculenta (crantz) were assessed by aflps for genetic diversity and for resistance to cbb. aflp analysis was performed using two primer combinations and a 79.2% level of polymorphism was found. the ... | 1999 | 10231955 |
| regiospecific glycosidase-assisted synthesis of lacto-n-biose i (galbeta1-3glcnac) and 3'-sialyl-lacto-n-biose i (neuacalpha2-3galbeta1-3glcnac). | the all-transglycolytic synthesis of lacto-n-biose i (galbeta1-3glcnac) and 3'-sialyl-lacto-n-biose i (neuacalpha2-3galbeta1-3glcnac) was performed. the disaccharide lacto-n-biose i was obtained by use of p-nitrophenyl beta-d-galactopyranoside as the donor, 2-acetamido-2-deoxy-d-glucopyranose as the acceptor and xanthomonas manihotis beta-d-galactosidase as the catalyst. the reaction was shown to be regiospecific, with a high molar yield (about 55%) with respect to the donor. lacto-n-biose i obt ... | 2000 | 10672000 |
| structure of the o-chain polysaccharide of the lipopolysaccharide of xanthomonas campestris pv. manihotis gspb 2755 and gspb 2364. | the o-chain polysaccharide of the lipopolysaccharide of xanthomonas campestris pv. manihotis strains gspb 2755 and gspb 2364 was studied by sugar and methylation analyses and 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments. the polysaccharide was found to contain l-rhamnose and l-xylose in the ratio 3:1, and the following structure of the tetrasaccharide repeating unit was established: [formula: see ... | 2000 | 10782308 |
| the identification of the catalytic nucleophiles of two beta-galactosidases from glycoside hydrolase family 35. | the beta-galactosidases from xanthomonas manihotis (beta-gal xmn) and bacillus circulans (beta-gal-3 bcir) are retaining glycosidases that hydrolyze glycosidic bonds through a double displacement mechanism involving a covalent glycosyl-enzyme intermediate. the mechanism-based inactivator 2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-d-galactopyranoside was shown to inactivate beta-gal xmn and beta-gal-3 bcir through the accumulation of 2-deoxy-2-fluorogalactosyl enzyme intermediates with half lives of ... | 2001 | 11423106 |
| differential esterase expression in leaves of manihot esculenta crantz infected with xanthomonas axonopodis pv. manihotis. | the polyacrylamide gel electrophoresis system (page) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases in plants and to show a differential expression of esterases as markers of pathogenesis in cassava plants (manihot esculenta crantz). the characterization of alpha- and beta-esterases from leaves of m. esculenta by the page system was possible using an extraction solution containing two phenol-complexi ... | 2001 | 11758725 |
| characterization of pathogenic and nonpathogenic strains of xanthomonas axonopodis pv. manihotis by pcr-based dna fingerprinting techniques. | strains of xanthomonas axonopodis pv. manihotis (xam) were characterized for pathogenicity and for dna polymorphism using different pcr-based techniques. using amplified restriction fragment length polymorphism (aflp), strains were distinguished from each other and also from other xanthomonas strains. cluster analysis showed a high correlation between dna polymorphism and pathogenicity. four xam strains were further analyzed using three pcr-based techniques, aflp, aflp-pthb and rapd-pthb. variou ... | 2002 | 12393196 |
| genetic structure and population dynamics of xanthomonas axonopodis pv. manihotis in colombia from 1995 to 1999. | restriction fragment length polymorphisms (rflps) were used to study the population genetics and temporal dynamics of the cassava bacterial pathogen xanthomonas axonopodis pv. manihotis. the population dynamics were addressed by comparing samples collected from 1995 to 1999 from six locations, spanning four different edaphoclimatic zones (eczs). forty-five different x. axonopodis pv. manihotis rflp types or haplotypes were identified between 1995 and 1999. high genetic diversity of the x. axonop ... | 2004 | 14711649 |
| molecular genetic analyses of potential beta-galactosidase genes in xanthomonas campestris. | xanthomonas campestris pv. campestris, which displays no significant beta-1,4-d-galactopyranosidase activity, has three annotated beta-galactosidase genes in the sequenced genome, designated gala, galb and galc herein. gala and galb are similar to glycosyl hydrolase (gh) family 2 enzymes, including escherichia coli lacz. gala and galb cannot express detectable activity even after being cloned in-frame and driven by the vector's promoter. galc is a gh35 enzyme homologous to the xanthomonas axonop ... | 2003 | 15153767 |
| mechanism-based fluorescent labeling of beta-galactosidases. an efficient method in proteomics for glycoside hydrolases. | (4-n-5-dimethylaminonaphthalene-1-sulfonyl-2-difluoromethylphenyl)-beta-d-galactopyranoside was synthesized and successfully tested on beta-galactosidases from xanthomonas manihotis (wong-madden, s. t., and landry, d. glycobiology (1995) 5, 19-28 and taron, c. h., benner, j. s., hornstra, l. j., and guthrie, e. p. (1995) glycobiology 5, 603-610), escherichia coli (jacobson, r. h., zhang, x. j., dubose, r. f., and matthews, b. w. (1994) nature 369, 761-766), and bacillus circulans (fujimoto, h., ... | 2004 | 15308675 |
| a unigene catalogue of 5700 expressed genes in cassava. | two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ests. two libraries were constructed from cassava root tissues of varieties with high and low starch contents. other libraries were constructed from plant tissues challenged by the pathogen xanthomonas axonopodis pv.manihotis. we report here the single pass sequencing of 11,954 cdna clones from the 5' ends, including 111 from the 3' ends. cluster ... | 2004 | 15630618 |
| recent progress in the characterization of molecular determinants in the xanthomonas axonopodis pv. manihotis-cassava interaction. | cassava bacterial blight, caused by xanthomonas axonopodis pv. manihotis (xam), is a widespread disease that affects cassava (manihot esculenta crantz). studies on the pathogen population structure, pathogen diagnosis, identification and expression of plant genes involved in resistance have been carried out. different molecular techniques were developed to assess the genetic diversity among the xampopulations. characterization of xam population dynamics over time had enable us to determine the d ... | 2004 | 15630621 |
| gene expression profile in response to xanthomonas axonopodis pv. manihotis infection in cassava using a cdna microarray. | a cassava cdna microarray based on a large cassava est database was constructed and used to study the incompatible interaction between cassava and xanthomonas axonopodis pv. manihotis (xam) strain cio151. for microarray construction, 5700 clones from the cassava unigene set were amplified by polymerase chain reaction (pcr) and printed on glass slides. microarray hybridization was performed using cdna from cassava plants (resistant variety mbra685) collected at 12, 24, 48 h and 7 and 15 days post ... | 2005 | 15830129 |
| pathological and molecular characterization of xanthomonas campestris strains causing diseases of cassava (manihot esculenta). | fifty-one strains representing xanthomonas campestris pv. manihotis and cassavae and different pathovars occurring on plants of the family euphorbiaceae were characterized by ribotyping with a 16s+23s rrna probe of escherichia coli and by restriction fragment length polymorphism analysis with a plasmid probe from x. campestris pv. manihotis. pathogenicity tests were performed on cassava (manihot esculenta). histological comparative studies were conducted on strains of two pathovars of x. campest ... | 1994 | 16349463 |
| geographical differentiation of the population of xanthomonas axonopodis pv. manihotis in colombia. | [this corrects the article on p. 4430 in vol. 63.]. | 1998 | 16349520 |
| geographical differentiation of the population of xanthomonas axonopodis pv. manihotis in colombia. | analyses of dna polymorphism and virulence variation were used to evaluate the population structure of xanthomonas axonopodis pv. manihotis, the pathogen causing cassava bacterial blight in colombia. we collected strains from the major cassava-growing regions which can be grouped into different edaphoclimatic zones (eczs) according to environmental conditions, production constraints, and economic parameters. dna polymorphism was assessed by a restriction fragment length polymorphism analysis, us ... | 1997 | 16535731 |
| catalytic properties of a mutant beta-galactosidase from xanthomonas manihotis engineered to synthesize galactosyl-thio-beta-1,3 and -beta-1,4-glycosides. | the identity of the acid/base catalyst of the family 35 beta-galactosidases from xanthomonas manihotis (bgax) has been confirmed as glu184 by kinetic analysis of mutants modified at that position. the glu184ala mutant of bgax is shown to function as an efficient thioglycoligase, which synthesises thiogalactosides with linkages to the 3 and 4 positions of glucosides and galactosides in high (>80%) yields. kinetic analysis of the thioglycoligase reveals glycosyl donor k(m) values of 1.5-21 microm ... | 2006 | 16844121 |
| mapping est-derived ssrs and ests involved in resistance to bacterial blight in manihot esculenta. | cassava (manihot esculenta crantz) is a major root crop widely grown in the tropics. cassava bacterial blight, caused by xanthomonas axonopodis pv. manihotis (xam), is an important disease in latin america and africa resulting in significant losses. the preferred control method is the use of resistant genotypes. mapping expressed sequence tags (ests) and determining their co-localization with quantitative trait loci (qtls) may give additional evidence of the role of the corresponding genes in re ... | 2007 | 18059536 |
| identification of pathotypes of xanthomonas axonopodis pv. manihotis in africa and detection of quantitative trait loci and markers for resistance to bacterial blight of cassava. | abstract cassava suffers from bacterial blight attack in all growing regions. control by resistance is unstable due to high genotype-environment interactions. identifying genes for resistance to african strains of xanthomonas axonopodis pv. manihotis can support breeding efforts. five f(1) cassava genotypes deriving from the male parent 'cm2177-2' and the female parent 'tms30572' were used to produce 111 individuals by backcrossing to the female parent. in all, 16 genotypes among the mapping pop ... | 2004 | 18943797 |
| measuring the genetic diversity of xanthomonas axonopodis pv. manihotis within different fields in colombia. | abstract cassava bacterial blight, caused by xanthomonas axonopodis pv. manihotis, is a widespread disease that affects cassava (manihot esculenta). we collected 238 x. axonopodis pv. manihotis strains by intensively sampling single fields in four edaphoclimatic zones (eczs) in colombia. dna polymorphism of different x. axonopodis pv. manihotis populations was assessed by restriction fragment length polymorphism (rflp) analyses, repetitive sequence-based polymerase chain reaction (rep-pcr), and ... | 2000 | 18944486 |
| development of a new semiselective medium for isolating xanthomonas campestris pv. manihotis from plant material and soil. | abstract an effective control for bacterial blight of cassava (manihot esculenta), caused by xanthomonas campestris pv. manihotis, requires the use of non-contaminated cuttings and seeds. using classical agar plating techniques for screening planting material for contamination has not been very successful because of the lack of a reliable semiselective agar medium. the pathogen grows slowly on general plating media and is easily overgrown by saprophytic bacteria during isolation from diseased pl ... | 1999 | 18944695 |
| identification of genes in cassava that are differentially expressed during infection with xanthomonas axonopodis pv. manihotis. | summary the cdna-amplified fragment length polymorphism approach was used to identify differentially expressed transcripts from cassava infected by xanthomonas axonopodis pv. manihotis (xam). approximately 3600 transcript-derived fragments (tdfs) were screened of which 340 were isolated. the nucleotide sequences of 250 tdfs were analysed and assembled into contigs and singletons. the amino acid sequences of their predicted products were compared with entries in databases and 63 of these clones s ... | 2004 | 20565629 |
| cdna-aflp reveals genes differentially expressed during the hypersensitive response of cassava. | summary the tropical staple cassava is subject to several major diseases, such as cassava bacterial blight, caused by xanthomonas axonopodis pv. manihotis. disease-resistant genotypes afford the only practical solution, yet despite the global importance of this crop, little is known about its defence mechanisms. cdna-aflp was used to isolate cassava genes differentially expressed during the hypersensitive reaction (hr) of leaves in response to an incompatible pseudomonas syringae pathovar. seven ... | 2005 | 20565643 |