Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
fluorine content of vegetables and fruits.twenty-six species of vegetables grown in szeged, in its environment and at szabadkigyós were examined for their f content. the mean f content of the most important staple products is 0.30 mg/kg raw weight or less. when vegetables of the same kind were examined, the results were mostly different. the highest mean f content was obtained for spinach (0.55 mg); potato yielded less (0.15 mg), and the lowest was obtained for cucumber (0.06 mg). the mean f content of the 13 fruit species was 0.10 mg/k ...1978754476
[about the influence of frost periods upon the serological detection of prunus ring spot viruses in cherries (author's transl)].during three years serological tests (latex test) were run from novemeber till april to detect prunus ring spot viruses in forced buds of prunus avium l., p. avium l. var. avium, and p. cerasus l. it was found that prunus necrotic ring spot virus (nrv) could be detected reliably during the winter in all infected trees. in contrary the detection of prune dwarf virus (pdv) was affected by temperatures below zero. in 1971 a low percentage of positive reacting trees was pointed out after the frost p ...1977910571
[acquisition and study of callus tissue during cultivation of mazzard and strawberry anthers].anters of 7 varieties of cerasus avium (2n=16) and fragaria grandiflora (2n==56) were cultured on murashige-skoog's medium. formation of the callus tissue was observed for only one variety of sweat cherry with a frequency of 3.1-20.6%. the number of haploid cells in first transfers varied from 22 to 44% in different strains. there were two rises of mitotic activity in the callus cells. subsequent transfers caused an increase in haploid cells. chromosome aberrations frequency was comparatively lo ...20081025784
[analytic and biological standardization of prunus avium extracts].novicardine, the acetonic extract of p. avium peduncle which has advantageous cardiotonic effect, was standardized chemically and biologically. flavonones, flavones, isoflavones and their glycosides were (methanolbuffer solution eluent, gradient elution) detected by reverse phase hplc technique. it has been found that substances produced from different varieties of cherry peduncle originated from various lands, have the same components and their ratios are similar, but a contradictory statement ...19921442096
an alternative approach for gene transfer in trees using wild-type agrobacterium strains.micropropagated shoots of three forest tree species, poplar (populus tremula x p. alba), wild cherry (prunus avium l.) and walnut (juglans nigra x j. regia), were inoculated each with six different wild-type agrobacterium strains. poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. on poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic t-d ...19911653060
[in vitro regeneration and applications using vegetable cell and tissue culture].plant cells by means of their totipotency and aided by in vitro culture techniques can be induced to perform morphogenesis leading to somatic embryoids and massive clonal multiplication; microspores or pollen can be triggered to recover haploid plants, then characters expressed via haploidy can be selected and fixed. protoplasts from different species can lead to recombinations. we report here work done on carica pubescens, where somatic embryoids were obtained from cells; in prunus avium androg ...19902133516
outer membrane protein mediating iron uptake via pyoverdinpss, the fluorescent siderophore produced by pseudomonas syringae pv. syringae.in an iron-limited environment pseudomonas syringae pv. syringae b301d produces a yellow-green fluorescent siderophore called pyoverdinpss which functions in high-affinity iron transport. two-dimensional electrophoretic comparisons of the outer membrane proteins of strain b301d identified nine proteins which were expressed at low (50 nm) but not at high (10 microm) iron concentrations. except for the minor protein 8e, the iron-regulated proteins exhibited high molecular weights ranging from appr ...19873032911
plant virus detection using a new form of indirect elisa.a novel form of indirect enzyme-linked immunosorbent assay (elisa) has been devised for the detection of viruses in plants. the method uses protein a in two applications to sandwich antibody-antigen-antibody layers. the first applied layer of protein a prepares the plate for the coating antibody layer. the second layer of protein a is conjugated to the enzyme and detects the second antibody layer. the orientation of the igg induced in the coating layer of antibody prevents later unwanted reactio ...19854055975
inhibition by carboxylic acids of an o-diphenol oxidase from prunus avium fruits. 19744208079
pharmacology of cherry (prunus avium) stalk extract. i. effect on smooth muscle. 19695791461
pharmacology of cherry (prunus avium) stalk extract. ii. cardiovascular effects. 19695791462
[biogenesis of plant pigments. ii. on the biogenesis of flavanones and flavanonols of robinia pseudoacacia, prunus avium and prunus mahaleb]. 19676056735
cytological zonation of prunus avium l. pollen tubes in vivo. 19807381990
cherry virus a: cdna cloning of dsrna, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry.the nucleotide sequence (7383 nucleotides) of a newly identified member of the genus capillovirus, cherry virus a (cva), was obtained from cdna clones. the cdna was generated from dsrna extracted from plant tissue infected with little cherry virus (lcv). small amounts of lcv dsrna served as template nucleic acid and enabled the construction of a library of which, unexpectedly, 7.5% of the recombinant plasmids were specific for cva. the genome organization of cva resembles that of apple stem groo ...19957636482
anti-inflammatory effects of cherry (prunus avium l.) stalk extract. 19948073068
a linkage map of sweet cherry based on rapd analysis of a microspore-derived callus culture population.a partial linkage map was constructed for the sweet cherry (prunus avium l.) cultivar emperor francis from a population of 56 microspore-derived callus culture individuals. the callus cultures were genotyped for two allozymes and 90 random amplified polymorphic dna (rapd) markers using 79 random decanucleotide dna primers and the polymerase chain reaction (pcr). eighty-nine markers mapped to 10 linkage groups totaling 503.3 cm. dna blot and hybridization analysis using five cloned rapds as probe ...20108683097
a cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like.a 29-kd polypeptide is the most abundant soluble protein in ripe cherry fruit (prunus avium l); accumulation begins at the onset of ripening as the fruit turns from yellow to red. this protein was extracted from ripe cherries and purified by size-exclusion and ion-exchange chromatography. antibodies to the purified protein were used to screen a cdna library from ripe cherries. numerous recombinant plaques reacted positively with the antibodies; the dna sequence of representative clones encoded a ...19968685266
molecular cloning, expression and characterization of pru a 1, the major cherry allergen.a high percentage of birch pollen allergic patients experiences food hypersensitivity reactions after ingestion of several fruits and vegetables. previous work demonstrated common epitopes on an allergen of mr 18,000 from sweet cherry (prunus avium) and bet v 1, the major allergen from birch pollen. n-terminal amino acid sequencing showed a sequence identity of 67% with bet v 1. here we report the cloning and cdna sequencing of this cherry allergen. the entire deduced amino acid sequence describ ...19979393965
cytological comparison of leaves and stems of prunus avium l. shoots cultured on a solid medium with agar or gelrite.an axillary proliferating clone of prunus avium l. was subcultured every four weeks on solid ms medium with agar as the gelling agent. vitrification (hyperhydricity) of shoots was induced in one four week cycle with the same medium except that agar was replaced by gelrite. during culture on the vitrifying medium, the water content of the shoots progressively increased with a parallel decrease in chlorophyll content. cytological differences between the leaves and stems of the vitrified and normal ...19989554582
biochemical characterization of pru a 2, a 23-kd thaumatin-like protein representing a potential major allergen in cherry (prunus avium).the prevalence of allergy to fruits and vegetables increased with pollinosis over the last 10 years. so far, clusters of hypersensitivity have been established and corroborated by the molecular characterization of individual cross-reacting allergens. several case studies demonstrated the existence of allergic reactions to fruits of the subfamily prunoideae (apricots, cherries, plums and peaches). here, we present the characterization of a major allergen in cherry.19989623505
virulence and molecular polymorphism of prunus necrotic ringspot virus isolates.prunus necrotic ringspot virus (pnrsv) occurs as numerous strains or isolates that vary widely in their pathogenic, biophysical and serological properties. prior attempts to distinguish pathotypes based upon physical properties have not been successful; our approach was to examine the molecular properties that may distinguish these isolates. the nucleic acid sequence was determined from 1.65 kbp rt-pcr products derived from rna 3 of seven distinct isolates of pnrsv that differ serologically and ...19989680147
detection of plum pox virus by enzyme-linked immunosorbent assay in some apricot and peach varieties and hybrids in romania.plum pox virus (ppv) is a potyvirus widely spread in many species of the prunus genus such as plum, apricot, peach, sweet cherry and others. this potyvirus causes great damage to stone fruit trees in romania and other european countries as hungary, italy, czech republic, france, spain, greece, turkey, and slovak republic. the research station for fruit tree growing baneasa in bucharest has realized many studies on the epidemiology and spread of ppv and also on the disease symptomatology and dete ...199810073239
purification, partial amino acid sequence and structure of the product of raucaffricine-o-beta-d-glucosidase from plant cell cultures of rauwolfia serpentina.plant cell suspension cultures of rauwolfia produce within 1 week approximately 250 nkat/l of raucaffricine-o-beta-d-glucosidase. a five step procedure using anion exchange chromatography, chromatography on hydroxylapatite, gel filtration and fplc-chromatography on mono q and mono p delivered in a yield of 0.9% approximately 1200-fold enriched glucosidase. a short protocol employing deae sepharose, tsk 55 s gel chromatography and purification on mono q gave a 5% recovery of glucosidase which was ...199910234858
cross-reactivity and epitope analysis of pru a 1, the major cherry allergen.a high percentage of birch pollen allergic patients experiences food hypersensivity after ingestion of fresh fruits and vegetables. the cross-reactivity of the major allergens of sweet cherry (pru a 1), apple (mal d 1), pear (pyr c 1), celery tuber (api g 1) and carrot (dau c 1) is due to structural similarities which are reflected by high amino acid sequence identities with bet v 1a, the major birch pollen allergen. apart from a strong cross-reactivity to bet v 1a, ige inhibition experiments wi ...199910403481
differentiation of closely related but biologically distinct cherry isolates of prunus necrotic ringspot virus by polymerase chain reaction.prunus necrotic ringspot ilarvirus (pnrsv) exists as a number of biologically distinct variants which differ in host specificity, serology, and pathology. previous nucleotide sequence alignment and phylogenetic analysis of cloned reverse transcription-polymerase chain reaction (rt-pcr) products of several biologically distinct sweet cherry isolates revealed correlations between symptom type and the nucleotide and amino acid sequences of the 3a (putative movement protein) and 3b (coat protein) op ...199910471030
physicochemical characteristics of selected sweet cherry cultivars.the volatile, sugar, and organic acid constituents in 12 cultivars and selections of sweet cherries (prunus avium l.) were characterized and quantified by high-performance liquid chromatography and gas chromatography (gc). fruit weight, soluble solids concentration (ssc), ph, titratable acidity (ta), and color (cie l, a, b) were also determined at harvest. weight ranged from 8.8 to 14.5 g per fruit, ssc from 13.5 to 24.5 degrees brix, and ssc/ta ratio from 18.3 to 29.0. chroma was a better indic ...199810554265
gibberellins in seedlings and flowering trees of prunus avium l.extracts of acids from mature seeds, germinating seeds, first, second and third year seedlings as well as mature, flowering trees of sweet cherry (prunus avium l. cv. stella) were analysed by gas chromatography-mass spectrometry. the presence of the known gibberellins (gas) ga1 (1), ga3 (4), ga5 (7), ga8 (11), ga19 (14), ga20 (12), ga29 (13), ga32 (5), ga85 (2), ga86 (3) and ga87 (6) was confirmed by comparison of their mass spectra and kovats retention indices with those of standards or literat ...200010731033
chloroplast inheritance and dna variation in sweet, sour, and ground cherry.sour cherry (prunus cerasus l.) is an allotetraploid and both sweet cherry (p avium l.) and ground cherry (p. fruticosa pall.) are the proposed progenitor species. the study investigated the maternal species origin(s) of sour cherry using chloroplast dna (cpdna) markers and a diverse set of 22 sweet, 25 sour, and 7 ground cherry selections. two cpdna restriction fragment length polymorphisms (rflps) and one polymerase chain reaction (pcr) fragment length polymorphism were identified among the 54 ...200810739133
phenology in central europe--differences and trends of spring phenophases in urban and rural areas.in order to examine the impacts of both large-scale and small-scale climate changes (urban climate effect) on the development of plants, long-term observations of four spring phenophases from ten central european regions (hamburg, berlin, cologne, frankfurt, munich, prague, vienna, zurich, basle and chur) were analysed. the objective of this study was to identify and compare the differences in the starting dates of the pre-spring phenophases, the beginning of flowering of the snowdrop (galanthus ...200010993559
metabolism of gibberellins a1 and a3 in fruits and shoots of prunus avium.isotope-labelled ga metabolites were identified by gc--ms, following hplc fractionation of extracts derived from fruits or shoots, that had been incubated with [2h]- and [3h]- ga1 or [2h]- and [3h]- ga3. ga1 (1) was converted into ga8 (10) by developing fruits and vegetative shoots of sweet cherry (prunus avium cv. 'stella'), while ga3 (4) was converted into ga3-isolactone (17). other metabolites of each ga were detected but were not identified unequivocally. these included a metabolite of ga1 ( ...200111198821
ozone exposure thresholds and foliar injury on forest plants in switzerland.canton ticino in southern switzerland is exposed to some of the highest concentrations of tropospheric ozone in europe. during recent field surveys in canton ticino, foliar symptoms identical to those caused by ozone have been documented on native tree and shrub species. in europe, the critical ozone level for forest trees has been defined at an aot40 of 10 ppm.h o3 (10 ppm.h accumulated exposure of ozone over a threshold of 40 ppb) during daylight hours over a six-month growing season. the obje ...200111202736
studies on water transport through the sweet cherry fruit surface: characterizing conductance of the cuticular membrane using pericarp segments.water conductance of the cuticular membrane (cm) of mature sweet cherry fruit (prunus avium l. cv. sam) was investigated by monitoring water loss from segments of the outer pericarp excised from the cheek of the fruit. segments consisted of epidermis, hypodermis and several cell layers of the mesocarp. segments were mounted in stainless-steel diffusion cells with the mesocarp surface in contact with water, while the outer cuticular surface was exposed to dry silica (22 +/- 1 degrees c). conducta ...200011219577
the contribution of syringopeptin and syringomycin to virulence of pseudomonas syringae pv. syringae strain b301d on the basis of sypa and syrb1 biosynthesis mutant analysis.sequencing of an approximately 3.9-kb fragment downstream of the syrd gene of pseudomonas syringae pv. syringae strain b301d revealed that this region, designated sypa, codes for a peptide synthetase, a multifunctional enzyme involved in the thiotemplate mechanism of peptide biosynthesis. the translated protein sequence encompasses a complete amino acid activation module containing the conserved domains characteristic of peptide synthetases. analysis of the substrate specificity region of this m ...200111277431
purification and characterisation of a beta-glucosidase abundantly expressed in ripe sweet cherry (prunus avium l.) fruit.a beta-glucosidase (beta-d-glucoside glucohydrolase, ec 3.2.1.21) was purified to homogeneity from ripe fruits of sweet cherry (prunus avium l.) by ammonium sulphate precipitation, ion exchange and size exclusion chromatography. the enzyme is a monomer with a molecular mass of approximately 68 kda and an acidic isoelectric point. n-terminal sequence analysis indicated that sweet cherry beta-glucosidase is related to other plant cyanogenic beta-glucosidases. substrate specificity studies revealed ...200111297776
are hyperhydric shoots of prunus avium l. energy deficient?the content of oxidized and reduced pyridine nucleotides and some enzymatic activities of the oxidative pentose phosphate and glycolytic pathways were compared in normal (ns, growing on agar) and hyperhydric (hs, growing on gelrite) shoots of prunus avium l. after 4 weeks of in vitro culture. the chlorophyll fluorescence from leaves and the redox capacity of the plasma membrane (reduction of exogenously added ferricyanide) of both types of shoots were recorded. the pool of oxidized and reduced p ...200111337071
variation of gas exchange within native plant species of switzerland and relationships with ozone injury: an open-top experiment.gas exchange and ozone-induced foliar injury were intensively measured during a 6-day period in mid-august 1998 on leaves of acer pseudoplatanus, betula pendula, corylus avellana, fagus sylvatica, fraxinus excelsior, morus nigra, prunus avium, prunus serotina, rhamnus cathartica, and viburnum lantana at a forest nursery site in canton ticino, switzerland. plants were grown in four open plots (aa), four open-top chambers receiving carbon-filtered (cf) air, and four receiving non-filtered (nf) air ...200111383335
seasonal variation of monoterpene emission from malus domestica and prunus avium.emission rates of monoterpenes released by apple (malus domestica borkh) and cherry (prunus avium l.) were estimated at different phenological stages. these measurements employed a dynamic flow-through teflon chamber, sample collection onto cartridges filled with graphitized carbon and thermal desorption gas chromatography-mass spectrometry (gc-ms) for identification and quantification of the emitted volatiles. at full bloom the release of monoterpene hydrocarbons from cherry flowers was 1213 ng ...200111397434
sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection by rt-pcr.phenolic compounds from plant tissues inhibit reverse transcription-polymerase chain reaction (rt-pcr). multiple-step protocols using several additives to inhibit polyphenolic compounds during nucleic acid extraction are common, but time consuming and laborious. the current research highlights that the inclusion of 0.65 to 0.70% of sodium sulphite in the extraction buffer minimizes the pigmentation of nucleic acid extracts and improves the rt-pcr detection of potato virus y (pvy) and potato leaf ...200211684310
cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries.anthocyanins from tart cherries, prunus cerasus l. (rosaceae) cv. balaton and montmorency; sweet cherries, prunus avium l. (rosaceae); bilberries, vaccinum myrtillus l. (ericaceae); blackberries, rubus sp. (rosaceae); blueberries var. jersey, vaccinium corymbosum l. (ericaceae); cranberries var. early black, vaccinium macrocarpon ait. (ericaceae); elderberries, sambucus canadensis (caprifoliaceae); raspberries, rubus idaeus (rosaceae); and strawberries var. honeoye, fragaria x ananassa duch. (ro ...200111695879
studies on water transport through the sweet cherry fruit surface: ii. conductance of the cuticle in relation to fruit development.water conductance of the cuticular membrane (cm) of sweet cherry (prunus avium l. cv. sam) fruit during stages ii and iii (31-78 days after full bloom, dafb) was investigated by gravimetrically monitoring water loss through segments of the exocarp. segments were mounted in stainless-steel diffusion cells, filled with 0.5 ml of deionized water and incubated for 8 h at 25 +/- 2 degrees c over dry silica. conductance was calculated by dividing the amount of water transpired per unit surface area an ...200111722129
a population genetic analysis of chloroplast dna in wild populations of prunus avium l. in europe.a population genetic study of chloroplast dna was carried out in 23 wild populations of prunus avium sampled from several european deciduous forests. an analysis of approx. 9% of the chloroplast genome detected mostly insertion-deletion mutations and one point mutation. in all, 16 haplotypes were detected. six haplotypes were shared by two or more populations and 10 were unique. one haplotype was present in 21 of the 23 populations and 161 of 211 individuals, which probably indicates its ancient ...200111737289
radiocaesium contamination of meadow vegetation--time-dependent variability and influence of soil characteristics at grassland sites in austria.long-term trends of 137cs and 40k concentrations in meadow grass and soil-plant transfer data at eight different sites in upper austria are presented. geometric means of 137cs tf-data and tagg values vary between 0.03-1.06 and 0.0005-0.0184 depending on site, respectively. 40k results are less variable with tf values covering a range of 0.31-2.01. only at one site was a significant decrease of 137cs concentration (decay-corrected) in meadow vegetation observed during the observation period 1992- ...200211814163
evolutionary analysis of s-rnase genes from rosaceae species.eight new cdna sequences for s-rnases were cloned and analysed from almond (prunus dulcis) cultivars of european origin, and compared to published sequences from other rosaceae species. insertions/deletions of 10-20 amino acid residues were detected in the rc4 and c5 domains of s-rnases from almond and sweet cherry. the s-rnases of the prunus species and those of the genera malus and pyrus formed two distinct groups on phylogenetic analysis. nucleotide substitutions were analysed in the s-rnase ...200211919717
using survival analysis to investigate the effect of uv-c and heat treatment on storage rot of strawberry and sweet cherry.ultraviolet light and heat treatment are proposed as alternative techniques for the use of chemicals to reduce the development of the spoilage fungi botrytis cinerea and monilinia fructigena on strawberry and sweet cherry, respectively, during storage. in order to investigate the effect of both physical techniques on microbial inactivation and on fruit quality, inoculated berries were subjected to different temperatures (40-48 degrees c) and uv-c doses (0.05-1.50 j/cm2). for each condition, 20 b ...200211934026
effect of cold treatment on survival and development of codling moth (lepidoptera: tortricidae) in cherry.'bing' cherries, prunus avium l., were obtained from an organic orchard and a conventional commercial orchard. the two groups were examined separately in replicated tests infested with each fruit initially infested with a first-instar codling moth, cydia pomonella (l.). to simulate commercial postharvest holding conditions, the treatments were 0 (control), 1, 2, 4, 7, 10, and 14 d cold storage at 3.3 degrees c. the fruits were examined three or more times to determine larval survival, life stage ...200211942758
studies on water transport through the sweet cherry fruit surface: iii. conductance of the cuticle in relation to fruit size.rain-cracking of sweet cherry fruit has been related to water absorption through the fruit surface and large fruit has been reported to be more susceptible to cracking than small fruit. therefore, the effect of fruit size on water conductance of the cuticular membrane (cm) of exocarp segments excised from cheek, suture or stylar end region of mature sweet cherry fruit (prunus avium l. cv. sam) was investigated. segments consisting of epidermis, hypodermis and several layers of mesocarp cells wer ...200212060264
molecular characterisation of sweet cherry (prunus avium l.) genotypes using peach [prunus persica (l.) batsch] ssr sequences.a total of 76 sweet cherry genotypes were screened with 34 microsatellite primer pairs previously developed in peach. amplification of ssr loci was obtained for 24 of the microsatellite primer pairs, and 14 of them produced polymorphic amplification patterns. on the basis of polymorphism and quality of amplification, a set of nine primer pairs and the resulting 27 informative alleles were used to identify 72 genotype profiles. of these, 68 correspond to unique cultivar genotypes, and the remaini ...200212080370
analysis of sweet cherry (prunus avium l.) leaves for plant signal molecules that activate the syrb gene required for synthesis of the phytotoxin, syringomycin, by pseudomonas syringae pv syringae.an important aspect of the interaction of pseudomonas syringae pv syringae with plant hosts is the perception of plant signal molecules that regulate expression of genes, such as syrb, required for synthesis of the phytotoxin, syringomycin. in this study, the leaves of sweet cherry (prunus avium l.) were analyzed to determine the nature of the syrb-inducing activity associated with tissues of a susceptible host. crude leaf extracts yielded high amounts of total signal activity of more than 12,00 ...199512228385
the distribution of little cherry disease in germany: lchv1 and lchv2 in cultivars of sweet cherry and in some other species. 200112425030
studies on water transport through the sweet cherry fruit surface. 7. fe3+ and al3+ reduce conductance for water uptake.the effects of the chloride salts licl, cacl(2), mgcl(2), alcl(3), eucl(3), and fecl(3) and the iron salts fecl(2), fecl(3), fe(no(3))(3), feso(4), and fe(2)(so(4))(3) on water conductance of exocarp segments (es) and rates of water uptake into detached sweet cherry fruit (prunus avium l. cv. adriana, early rivers, namare, namosa, and sam) were studied. es were excised from the cheek of mature fruit and mounted in stainless steel diffusion cell; water penetration was monitored gravimetrically fr ...200212475277
development of microsatellite markers in peach [ prunus persica (l.) batsch] and their use in genetic diversity analysis in peach and sweet cherry ( prunus avium l.).we report the sequence of 41 primer pairs of microsatellites from a ct-enriched genomic library of the peach cultivar 'merrill o'henry'. ten microsatellite-containing clones had sequences similar to plant coding sequences in databases and could be used as markers for known functions. for microsatellites segregating at least in one of the two prunus f(2) progenies analyzed, it was possible to demonstrate mendelian inheritance. microsatellite polymorphism was evaluated in 27 peach and 21 sweet che ...200212582570
differences in root longevity of some tree species.although the importance of root production and mortality to nutrient fluxes in ecosystems is widely recognized, the difficulties associated with root measurements have limited the availability of reliable data. we have used minirhizotrons and image analysis to measure root longevity of prunus avium l., picea sitchensis (bong.) carrière, acer pseudoplatanus l. and populus x canadensis cv. beaupre directly in cohorts of roots. major differences in the longevity of roots among species were identifi ...199812651380
characterization of microsatellites in wild and sweet cherry (prunus avium l.)--markers for individual identification and reproductive processes.nuclear microsatellites were characterized in prunus avium and validated as markers for individual and cultivar identification, as well as for studies of pollen- and seed-mediated gene flow. we used 20 primer pairs from a simple sequence repeat (ssr) library of prunus persica and identified 7 loci harboring polymorphic microsatellite sequences in p. avium. in a natural population of 75 wild cherry trees, the number of alleles per locus ranged from 4 to 9 and expected heterozygosity from 0.39 to ...200312669801
identification of a non-s rnase, a possible ancestral form of s-rnases, in prunus.this study identifies and characterizes a basic non-s rnase in the styles with stigmas of sweet cherry (prunus avium l.), a member of the rosaceae subfamily amygdaloideae, which has an rnase-based gametophytic self-incompatibility system. internal sequences of putative non-s rnases (rnase pa1 and pa2) were determined, and a cdna for pa1 was obtained. the deduced amino acid sequence of pa1 contained two conserved sequence motifs essential for t2/ s-type rnase activity. pa1 shows 20-30% sequence i ...200312715157
characterization of the s-rnase promoters from sweet cherry (prunus avium l.).genomic dna fragments containing the s(3)-, s(4)-, and s(6)-rnase genes were isolated from the sweet cherry (prunus avium l.) and sequenced. comparison of the 5'-flanking sequences of these three s-rnases indicated that a highly conserved region (designated cr) existed just upstream from the putative tata boxes. we postulate that cr contains cis-regulatory element(s) involved in pistil expression. to examine the activity of the isolated s-rnase promoters of sweet cherry in the pistil, we transie ...200312773819
ozone air pollution and foliar injury development on native plants of switzerland.the objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. the study was conducted from the beginning of may through the end of august during 2000 and 2001 using an open-top chamber research facility located within the lattecaldo cantonal forest nursery in canton ticino, southern switzerland (600 m asl) ...200312804826
a pollen-expressed gene for a novel protein with an f-box motif that is very tightly linked to a gene for s-rnase in two species of cherry, prunus cerasus and p. avium.this study describes a novel f-box protein gene in the s-locus of sour cherry (prunus cerasus) and sweet cherry (p. avium). the gene showed an s-haplotype-specific sequence polymorphism and the expression was specific to pollen. genomic dna blot analysis of eight sweet cherry cultivars with the probe for the f-box protein gene under low stringency conditions yielded rflp bands specific to the s-haplotypes of each cultivar. we discuss the possibility of the gene for the f-box protein being a cand ...200312881505
mutational epitope analysis of pru av 1 and api g 1, the major allergens of cherry (prunus avium) and celery (apium graveolens): correlating ige reactivity with three-dimensional structure.birch pollinosis is often accompanied by adverse reactions to food due to pollen-allergen specific ige cross-reacting with homologous food allergens. the tertiary structure of pru av 1, the major cherry (prunus avium) allergen, for example, is nearly identical with bet v 1, the major birch (betula verrucosa) pollen allergen. in order to define cross-reactive ige epitopes, we generated and analysed mutants of pru av 1 and api g 1.0101, the major celery (apium graveolens) allergen, by immunoblotti ...200312943529
cloning and characterization of genomic dna sequences of four self-incompatibility alleles in sweet cherry ( prunus avium l.).gametophytic self-incompatibility (gsi) in sweet cherry is determined by a locus s with multiple alleles. in the style, the s-locus codifies for an allele-specific ribonuclease ( s-rnase) that is involved in the rejection of pollen that carries the same s allele. in this work we report the cloning and genomic dna sequence analysis including the 5' flanking regions of four s-rnases of sweet cherry ( prunus avium l., rosaceae). dna from the cultivars ferrovia, pico colorado, taleguera brillante an ...200412955210
recycling of nitrogen in the xylem of prunus avium trees starts when spring remobilization of internal reserves declines.nitrogen (n) storage capacity of cherry (prunus avium l.) trees grown in sand culture was preconditioned by applying contrasting n supplies for one year. during the spring of the following year, a constant amount of 15n was supplied and the dynamics of n remobilization and root uptake were characterized as a function of internal n status of the trees. to calculate the flux of n through xylem, both xylem sap n concentration and whole-tree transpiration rates were measured. by comparing the cumula ...200312975130
[indeterminate chemistry of wood extracts. iv. new flavone derivatives from the heart of the wild cherry tree (prunus avium)]. 195413219528
[biochemistry of the sap of the wild cherry prunus avium]. 195513240336
[relation between the calcium and mineral phosphate content of the soil and the content of crude and elaborated sap of the wild cherry tree (prunus avium)]. 195513260867
[flavone compounds of wood extracts. v. water-soluble derivatives of sweet cherry heart (prunus avium)]. 195713437004
[biosynthesis of flavone derivatives in prunus avium. i. incorporation of potassium trans-3-c-14-cinnamate]. 195813596815
[isolation and determination of the structure of a new hydroxyflavanone from the cherry-tree heartwood (prunus avium)]. 195813608197
effect of selection on the frequency of genotypes determining incompatibility in prunus avium. 196013844941
direct observations of the redox states of frozen cherry buds by a unique in vivo esr.low temperatures cause cellular damage in flower buds of the sweet cherry (prunus avium l. cv. satohnishiki). in this study, the redox states within the cherry buds suffering freezing damage were non-destructively observed by a unique in vivo electron spin resonance (esr) technique with a spin probe such as carbamoyl-proxyl. the esr signals of carbamoyl-proxyl-treated bud were continuously recorded under freezing and thawing condition, which was decreased to approximately -4 degrees c and mainta ...200314511650
allele-specific pcr detection of sweet cherry self-incompatibility (s) alleles s1 to s16 using consensus and allele-specific primers.pcr-based identification of all 13 known self-incompatibility (s) alleles of sweet cherry is reported. two pairs of consensus primers were designed from our previously published cdna sequences of s(1) to s(6) s-rnases, the stylar components of self-incompatibility, to reveal length variation of the first and the second introns. with the exception of the first intron of s(13), these also amplified s(7) to s(14) and an allele previously referred to as s(x), which we now label s(16). the genomic pc ...200314523529
flavanol binding of nuclei from tree species.light microscopy was used to examine the nuclei of five tree species with respect to the presence of flavanols. flavanols develop a blue colouration in the presence of a special p-dimethylaminocinnamaldehyde (dmaca) reagent that enables those nuclei loaded with flavanols to be recognized. staining of the nuclei was most pronounced in both tsuga canadensis and taxus baccata, variable in metasequoia glyptostroboides, faint in coffea arabica and minimal in prunus avium. hplc analysis showed that th ...200414595514
a cdna encoding a cold-induced glycine-rich rna binding protein from prunus avium expressed in embryonic axes.a cdna clone encoding a presumed full-length glycine-rich ribonucleic acid (rna) binding protein was isolated from a lambda-zap express cdna library generated from primarily nondormant prunus avium (wild cherry) embryonic axes. the cdna, designated pa-rrm-grp1 (prunus avium rna recognition motif glycine-rich protein 1), contains a single n-terminal rna recognition motif (rrm) and single c-terminal glycine-rich domain. the glycine-rich domain is unusually long at 91 amino acids, 58 of which are g ...200314597401
correlating ige reactivity with three-dimensional structure.this commentary discusses the work of neudecker et al. in this issue of the biochemical journal in which site-directed mutagenesis and nmr spectroscopy have been used to analyse in detail the ige-binding capacity of two cross-reactive allergens: apg1.0101 from celery ( apium graveolens ) and pru av 1 from cherry ( prunus avium ), which are both members of the pathogenesis-related allergen family. the study, showing that the ige-binding epitopes are highly patient specific, will have a profound i ...200314602045
variation in the composition of the heartwood flavonoids of prunus avium by on-column capillary gas chromatography.a fast and simple extraction procedure, coupled with on-column gas chromatography, has been developed in order to investigate the relative amounts of flavonoids in individual trees within several homogeneous populations of prunus avium. eight known flavonoid aglycones were isolated from the heartwood of p. avium and multivariate analysis was employed in order to characterise population groups.200614667064
identification of incompatibility alleles in the tetraploid species sour cherry.the incompatibility genetics of sour cherry ( prunus cerasus), an allotetraploid species thought to be derived from sweet cherry (diploid) and ground cherry (tetraploid), were investigated by test crossing and by analysis of stylar ribonucleases which are known to be the products of incompatibility alleles in sweet cherry. stylar extracts of 36 accessions of sour cherry were separated electrophoretically and stained for ribonuclease activity. the zymograms of most accessions showed three bands, ...200414689184
effect of ripeness and postharvest storage on the phenolic profiles of cherries (prunus avium l.).the phenolic compounds hydroxycinnamates, anthocyanins, flavonols, and flavan-3-ols of sweet cherry cultivars burlat, saco, summit, and van harvested in 2001 and 2002 were quantified by hplc-dad. phenolics were analyzed at partially ripe and ripe stages and during storage at 15 +/- 5 degrees c (room temperature) and 1-2 degrees c (cool temperature). neochlorogenic and p-coumaroylquinic acids were the main hydroxycinnamic acid derivatives, but chlorogenic acid was also identified in all cultivars ...200414759143
effects of elevated co(2) on chloroplast components, gas exchange and growth of oak and cherry.specific chloroplast proteins, gas exchange and dry matter production in oak (quercus robur l.) seedlings and clonal cherry (prunus avium l. x pseudocerasus lind.) plants were measured during 19 months of growth in climate-controlled greenhouses at ambient (350 vpm) or elevated (700 vpm) co(2). in both species, the elevated co(2) treatment increased the ppfd saturated-rate of photosynthesis and dry matter production. after two months at elevated co(2), prunus plants showed significant increases ...199714759855
photoperiod effect on bud burst in prunus is phase dependent: significance for early photosynthetic development.a 16-h photoperiod stimulated bud burst in mature rooted cuttings of prunus avium cv. stella, but not in selfed stella seedlings. however, in a 12-h photoperiod, bud burst occurred earlier in the seedlings than in the mature cuttings. in the 12-h photoperiod, production of polypeptides involved in carbon dioxide fixation and photosynthetic electron transport was higher in seedlings than in mature cuttings, whereas in the 16-h photoperiod, shoot development and polypeptide production were similar ...199614871718
effects of elevated co(2) on growth and chloroplast proteins in prunus avium.to predict the future carbon sequestering capacity of trees, we need information about the possible acclimatory mechanisms of plant growth and photosynthesis in rising atmospheric co(2) under a variety of environmental conditions. we have, therefore, studied the growth response of a tree species (prunus avium l. stella (wild cherry)) to elevated co(2) and characterized the associated changes in photosynthetic machinery of the leaf tissue. self-pollinated seedlings and mature cuttings (clones) fr ...199414967647
rubisco and pep carboxylase responses to changing irradiance in a brazilian cerrado tree species, qualea grandiflora mart. (vochysiaceae).the activities of ribulose-1,5-bisphosphate carboxylase-oxygenase, rubisco (e.c. 4.1.1.39) and phosphoenolpyruvate carboxylase, pepc (e.c. 4.1.1.31), and concentrations of protein and chlorophyll were measured in extracts from cotyledons and first leaves of qualea grandiflora mart. (vochysiaceae) seedlings after transfer from high-light (20 days at 320 micro mol m(-2) s(-1), par) to low-light (35 days at 120 micro mol m(-2) s(-1), par) conditions. when tween 20 and glycerol were added to the ext ...199414967711
localization and quantitative determination of catechins and proanthocyanidins in the phloem of elm and cherry.the localization of catechins and proanthocyanidins in the phloem of elm (ulmus minor mill.) and cherry (prunus avium l.) was determined histochemically by use of 4-dimethylaminocinnamaldehyde (dmaca) reagent. the two tree species showed a characteristic distribution of these phenols, which were most abundant in the phloem rays of cherry, but were largely confined to upright parenchyma cells of elm. quantitative determination and qualitative separation of catechins and proanthocyanidins were per ...199214969867
selection for salt and drought tolerance in protoplast- and explant-derived tissue cultures of colt cherry (prunus avium x pseudocerasus).colt cherry (prunus avium x pseudocerasus) callus cultures were derived from leaf protoplasts, protoplasts of root cell suspension cultures, or by direct culture of leaf and root tissues. survival of calli cultured on basal proliferation medium containing 25, 50, 100 or 200 mn (millinormal) nacl, na(2)so(4) or kcl, or iso-osmotic (with nacl) concentrations of mannitol ranged from 1 to 15%. after six transfers on the same medium, surviving cell lines were subjected to three cycles of direct recur ...198914972993
changes in strain and deposition of cuticle in developing sweet cherry fruit.changes in surface area, deposition and elastic strain of the cuticular membrane (cm) were monitored during development of sweet cherry (prunus avium l.) fruit. fruit mass and surface area ('sam') increased in a sigmoidal pattern between 16 and 85 days after full bloom (dafb) with maximum rates of 0.35 g day(-1) and 0.62 cm(2) day(-1), respectively. rates of total area strain, namely the sum of elastic plus plastic strain, were highest in cheek and stem cavity regions followed by stylar and sutu ...200415032829
maillard reaction and enzymatic browning affect the allergenicity of pru av 1, the major allergen from cherry (prunus avium).the influence of thermal processing and nonenymatic as well as polyphenoloxidase-catalyzed browning reaction on the allergenicity of the major cherry allergen pru av 1 was investigated. after thermal treatment of the recombinant protein rpru av 1 in the absence or presence of carbohydrates, sds-page, enzyme allergosorbent tests, and inhibition assays revealed that thermal treatment of rpru av 1 alone did not show any influence on the ige-binding activity of the protein at least for 30 min, thus ...200415186129
hyperhydricity of prunus avium shoots cultured on gelrite: a controlled stress response.hyperhydricity is a physiological disorder frequently affecting shoots vegetatively propagated in vitro. hyperhydric shoots are characterised by a translucent aspect due to a chlorophyll deficiency, a not very developed cell wall and a high water content. hyperhydricity of prunus avium shoots was expressed in vitro in one multiplication cycle by replacing the gelling agent agar (normal shoots: ns) by gelrite (hyperhydric shoots: hs). p. avium shoots evolving towards the hyperhydric state produce ...200415246065
two-dimensional tension tests in plant biomechanics--sweet cherry fruit skin as a model system.splitting of fruits is a function of two-dimensional tension caused by different growth rates of tissues and turgor, especially water uptake shortly before harvest. in order to analyse the mechanical properties of spheroid plant material close to stress-strain conditions in vivo, a new hydraulic two-dimensional testing device was set up. sweet cherry (prunus avium l.) fruit skin was chosen as a model system. the recorded pressure-deflection curves were non-linear, with a considerable initial "la ...200415248126
the s haplotype-specific f-box protein gene, sfb, is defective in self-compatible haplotypes of prunus avium and p. mume.many prunus species, including sweet cherry and japanese apricot, of the rosaceae, display an s-rnase-based gametophytic self-incompatibility (gsi). the specificity of this outcrossing mechanism is determined by a minimum of two genes that are located in a multigene complex, termed the s locus, which controls the pistil and pollen specificities. sfb, a gene located in the s locus region, encodes an f-box protein that has appropriate s haplotype-specific variation to be the pollen determinant in ...200415272875
the role of polar auxin transport through pedicels of prunus avium l. in relation to fruit development and retention.it was investigated whether premature fruit abscission in prunus avium l. was triggered by a reduction in polar auxin transport (pat). the capacity of pedicels to transport tritiated iaa ([3h]-iaa) via the pat pathway was measured at intervals throughout flower and fruit development. the extent of passive diffusion, assessed by concurrent applications of [14c]-benzoic acid ([14c]-ba), was negligible. transported radioactivity recovered from agar blocks eluted at the same retention time as authen ...200415310825
molecular basis of pollen-related food allergy: identification of a second cross-reactive ige epitope on pru av 1, the major cherry (prunus avium) allergen.birch (betula verrucosa) pollen-associated food allergy is a well-characterized syndrome, which is due to the cross-reactivity of ige antibodies to homologous allergens in various foods. one crossreacting area on the major birch pollen allergen bet v 1 and its homologue in cherry (prunus avium) pru av 1 has already been identified. this is the so-called 'p-loop' region, which encompasses amino acid residues around position 45 and is found on the two virtually identical tertiary protein structure ...200515330760
studies on water transport through the sweet cherry fruit surface: ix. comparing permeability in water uptake and transpiration.water uptake and transpiration were studied through the surface of intact sweet cherry (prunus avium l.) fruit, exocarp segments (es) and cuticular membranes (cm) excised from the cheek of sweet cherry fruit and astomatous cm isolated from schefflera arboricola (hayata) hayata, citrus aurantium l., and stephanotis floribunda brongn. leaves or from lycopersicon esculentum mill. and capsicum annuum l. var. annuum fasciculatum group fruit. es and cm were mounted in diffusion cells. water (deionized ...200515338307
genetic relationships between diploid and allotetraploid cherry species (prunus avium, prunus x gondouinii and prunus cerasus).prunus avium l. (diploid, aa, 2n=2x=16), prunus cerasus l. (allotetraploid, aaff, 2n=4x=32) species, and their hybrid prunus x gondouinii rehd., constitute the most widely cultivated cherry tree species. p. cerasus is supposed to be an hybrid species produced by the union of unreduced p. avium gametes and normal p. fruticosa gametes. a continuum of morphological traits between these three species makes their assignation difficult. the aim of this paper is to study the genetic relationships betwe ...200415354194
expression of a sweet cherry dreb1/cbf ortholog in arabidopsis confers salt and freezing tolerance.dehydration responsive element binding protein 1 (dreb1)/c-repeat binding factor (cbf) induces the expression of many stress-inducible genes in arabidopsis. we have previously reported the identification of three dreb1/icbf homologs from sweet cherry (prunus avium). to identify the function of these homologs, one of the genes, cig-b, was transformed into arabidopsis. in one of the transgenic plant lines, the dreb1/cbf target gene cor15a was induced in the absence of stress treatment. the cor15a- ...200415535126
[identifying the s genotypes of sweet cherry (prunus avium l.) cultivars].this report identified s-rnase genes (s genes) of sweet cherry (prunus avium l.), presented the sequences of s genes by using a pair of specific primers pruc2 and pruc4r based on the conserved regions c2 and rc4 of rosaceous s-rnase genes and researched the s gene specific products from the genomic dnas of different cultivars in which most of the s genotypese were unknown. the bands of pcr were cloned and their sequences were compared in genbank. four s genes were defined and the conclusion was ...200415552051
loss of pollen-s function in two self-compatible selections of prunus avium is associated with deletion/mutation of an s haplotype-specific f-box gene.recently, an s haplotype-specific f-box (sfb) gene has been proposed as a candidate for the pollen-s specificity gene of rnase-mediated gametophytic self-incompatibility in prunus (rosaceae). we have examined two pollen-part mutant haplotypes of sweet cherry (prunus avium). both were found to retain the s-rnase, which determines stylar specificity, but one (s3' in ji 2434) has a deletion including the haplotype-specific sfb gene, and the other (s4' in ji 2420) has a frame-shift mutation of the h ...200515598801
characterization of pseudomonas pathovars isolated from rosaceous fruit trees in east algeria.a survey of bacterial diseases due to pseudomonas on rosaceous fruit trees was conducted. in forty two orchards located in the constantine region ( east algeria). pseudomonas isolates were identified on the bases of their cultural and biochemical characteristics . a total of fifty nine phytopathogenic bacteria were isolated from diseased pome and stone fruit trees. thirty one strains comparable to pseudomonas syringae pv. syringae were isolated from cherry (prunus avium l.), plum (p. domestica l ...200415756824
determining self-incompatibility genotypes in belgian wild cherries.abstract the self-incompatibility (s) genotypes of a collection of 65 belgian accessions of wild cherry, selected within two populations and planted in a seed orchard, were determined using polymerase chain reaction (pcr) methods. initially, dna extracts were amplified with consensus primers that amplify across the second intron of the s-ribonuclease gene which shows considerable length polymorphism. the provisional genotypes deduced were checked with the appropriate allele-specific primers for ...200515773927
chemical constituents and antioxidant activity of sweet cherry at different ripening stages.the development and ripening process of sweet cherry (prunus avium l. cv. 4-70) on the tree was evaluated. for this purpose, 14 different stages were selected in accordance with homogeneous size and color. some parameters related to fruit quality, such as color, texture, sugars, organic acids, total antioxidant activity, total phenolic compounds, anthocyanins, and ascorbic acid were analyzed. the results revealed that in sweet cherry, the changes in skin color, glucose and fructose accumulation, ...200515796619
in vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (prunus avium l.).regeneration of adventitious shoots from leaves and, for the first time, from internode sections were compared and optimized for five economically important sweet cherry cultivars, i.e. "schneiders", "sweetheart", "starking hardy giant", "kordia" and "regina" (prunus avium l.). the influence of basal media, carbon source, combination and dosage of phytohormones, ethylene inhibitor such as silver thiosulfate and a 16 h:8 h light:dark photoperiod versus complete darkness were evaluated. both, dkw/ ...200515948004
combined heat and controlled atmosphere quarantine treatments for control of codling moth in sweet cherries.nonchemical quarantine treatments, using a combination of short-duration high temperatures under low oxygen, elevated carbon dioxide atmospheric environment were developed to control codling moth in sweet cherries, prunus avium (l.). the two treatments developed are a chamber temperature of 45 degrees c for 45 min and a chamber temperature of 47 degreesc for 25 min under a 1% oxygen, 15% carbon dioxide, -2 degrees c dew point environment. both these treatments have been shown to provide control ...200516022297
influence of selected fruit tree pollen on life history of euseius stipulatus (acari: phytoseiidae).euseius stipulatus (athias-henriot) is a predatory mite widespread in the mediterranean region considered to be important for the biological control of spider mites in citrus orchards. development, survival and reproduction of this phytoseiid mite feeding on seven commercially obtained pollen were studied under constant laboratory conditions (20 +/- 1 degrees c, rh 65 +/- 5%, photoperiod 16l: 8d h). mites were kept individually at rearing units with ample quantity of almond (prunus amygdalus bat ...200516082919
scion-rootstock interaction affects the physiology and fruit quality of sweet cherry.water relations, leaf gas exchange, chlorophyll a fluorescence, light canopy transmittance, leaf photosynthetic pigments and metabolites and fruit quality indices of cherry cultivars 'burlat', 'summit' and 'van' growing on five rootstocks with differing size-controlling potentials that decrease in the order: prunus avium l. > cab 11e > maxma 14 > gisela 5 > edabriz, were studied during 2002 and 2003. rootstock genotype affected all physiological parameters. cherry cultivars grafted on invigorati ...200616203719
Displaying items 1 - 100 of 410