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mass spectrometric determination of amino acid sequence in cyl-2, a novel cyclotetrapeptide from cylindrocladium scoparium. 19744433711
genetic variability among isolates and sexual offspring of the plant pathogenic fungus calonectria morganii on the basis of random amplification of polymorphic dna (rapd) and restriction fragment length polymorphism (rflp)thirty-two strains of the phytopathogenic mold cylindrocladium scoparium (perfect state calonectria morganii) isolated from ericaceous hosts and two specimens from the atcc were examined by random amplification of polymorphic dna (rapd) and restriction fragment length polymorphism (rflp). five oligonucleotides were chosen as primers for differentiation of the isolates. rapd patterns of the atcc strains differed significantly from those of the field isolates. diversity among field isolates was lo ...19968824171
agrobacterium tumefaciens-mediated genetic transformation of the phytopathogenic ascomycete calonectria morganii.conidia of the phytopathogenic fungus calonectria morganii were transformed to hygromycin b resistance using the hph gene of escherichia coli as the selective trait, governed by a heterologous fungal promoter and the aspergillus nidulans trpc terminator. agrobacterium tumefaciens-mediated transformation yielded stable hygromycin b-resistant clones (average number (106 per 10(7)) [corrected] conidia). putative transformants appeared to be mitotically and meiotically stable. the presence of the hp ...200111680825
phytotoxic chaetoglobosins are produced by the plant pathogen calonectria morganii (anamorph cylindrocladium scoparium).by the application of a bioassay based on cresson seedlings, two phytotoxic compounds were isolated by thin-layer chromatography from the culture fluid of a calonectria morganii isolate. the structure of both compounds was elucidated by esi/ms and nmr spectroscopy. according to the chemical abstracts database, they were identified as chaetoglobosin a and 19-o-acetylchaetoglobosin a, mycotoxins originally described for chaetomium globosum.200112483566
activities and survival of endophytic bacteria in white clover (trifolium repens l.).in this study, the genera, abundance, and activities of endophytic bacteria in field-grown white clover (trifolium repens) and the fate of introduced antibiotic-tolerant bacteria in white clover tissues were investigated. pseudomonas, pantoea, and corynebacterium were the most frequently isolated endophytic bacteria genera, whereas xanthomonas, microbacterium, and cellulomonas occurred less frequently. the average bacterial populations in stolons and roots were approximately 100,000 colony-formi ...200617110977
characterization of a new antifungal lipid transfer protein from wheat.lipid transfer proteins (ltps) are members of the family of pathogenesis-related proteins (pr-14) that are believed to be involved in plant defense responses. in this study, a novel gene ltp 3f1 encoding an antifungal protein from wheat (sumai 3) was subcloned, overexpressed in escherichia coli bl-21 (de3) and enriched using ammonium sulfate fractionation followed by gel permeation chromatography. molecular phylogeny analyses of wheat ltp 3f1 gene showed a strong identity to other plant ltps. pr ...200818595724
rare or remarkable microfungi from oaxaca (south mexico)--part ii.microfungi were collected in southern mexico in the vicinity of puerto escondido, oaxaca in 2007. in 2006, samples were gathered from acacia myrmecophytes [(remarkable microfungi from oaxaca of acacia species) part i]. in the present investigation [part ii], we collected microfungi from different parts of a variety of wild and cultivated higher plants belonging to the families anacardiaceae, caricaceae, fabaceae, moraceae, and nyctaginacae. the microfungi found here live as parasites or saprophy ...200819226752
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