Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| genotyping isolates of the entomopathogenic fungus beauveria bassiana by rapd with fluorescent labels | random amplified polymorphic dna (rapd) with incorporation of fluorescent deoxynucleotides was used to examine the genetic diversity among beauveria bassiana isolates from argentina and brazil. high-resolution dna fingerprints were generated on line, during polyacrylamide gel electrophoresis of amplification products, by automated laser fluorescence analysis. each isolate displayed a distinct genotype. cluster analysis showed a high level of variability among these genotypes. no correlation with ... | 1998 | 9500948 |
| conventional resistance of experimental maize lines to corn earworm (lepidoptera: noctuidae), fall armyworm (lepidoptera: noctuidae), southwestern corn borer (lepidoptera: crambidae), and sugarcane borer (lepidoptera: crambidae). | plant resistance is a useful component of integrated pest management for several insects that are economically damaging to maize, zea mays l. in this study, 15 experimental lines of maize derived from a backcross breeding program were evaluated for resistance to corn earworm, helicoverpa zea (boddie); fall armyworm, spodoptera frugiperda (j. e. smith); southwestern corn borer, diatraea grandiosella dyar; and sugarcane borer, diatraea saccharalis (f.). experimental line 100-r-3 was resistant in t ... | 2000 | 10902359 |
| sampling for the sugarcane borer (lepidoptera: crambidae) on sugarcane in louisiana. | a 3-yr study was conducted in 0.6- to 2.0-ha sugarcane fields throughout south louisiana under varying sugarcane borer, diatraea saccharalis (f.), density levels to determine the spatial dispersion of infestations and to develop a sequential sampling plan. infestations of d. saccharalis were randomly dispersed. infestation levels (percentage of stalks infested) ranged from 0.6 to 33.3%. frequency distributions of the number of infested stalks indicated that the poisson distribution best fit the ... | 2001 | 11425035 |
| susceptibility of diatraea saccharalis (lepidoptera: crambidae) to tebufenozide. | dosage-mortality baselines were determined for first and newly molted third instars of the sugarcane borer, diatraea saccharalis (f.), with tebufenozide incorporated into an artificial diet. using death as an endpoint for these assays, lc50 and lc90 values were estimated to be 0.17 and 0.41 ppm for the first and 0.87 and 3.67 ppm for third instars, respectively. developmental effects also were observed and were defined to include mortality as well as physical impairment and retarded or prolonged ... | 2001 | 11777050 |
| selection, egg viability, and fecundity of the sugarcane borer (lepidoptera: crambidae) with tebufenozide. | two separate attempts to select the sugarcane borer, diatraea saccharalis (f.), for resistance to tebufenozide were unsuccessful. both selected colonies were lost after the fourth generation due to a lack of oviposition. differences were not detected in fecundity or percent egg viability for 5-d-old third instars exposed to concentrations (ec5, ec15, and ec30) of tebufenozide for 7 d. decreases (p < or = 0.01) in mean female pupal weights were detected in larvae exposed to ec15 and ec30 concentr ... | 2001 | 11777063 |
| evaluation of lectin-expressing transgenic sugarcane against stalkborers (lepidoptera: pyralidae): effects on life history parameters. | the impact of snowdrop lectin (galanthus nivalis agglutinin, gna) expressed in transgenic sugarcane on life history parameters of mexican rice borer [eoreuma loftini (dyar)] and sugarcane borer [diatraea saccharalis (f.)] (both lepidoptera: pyralidae) was evaluated. in the laboratory, lyophilized sugarcane leaf sheath tissue was incorporated in a meridic diet resulting in a gna concentration of 0.47% of total protein, and used for insect bioassays over two successive generations. deleterious eff ... | 2002 | 12020029 |
| identification, syntheses, and characterization of the geometric isomers of 9,11-hexadecadienal from female pheromone glands of the sugar cane borer diatraea saccharalis. | chemical analysis of the pheromone glands of the sugar cane borer diatraea saccharalis has shown the presence of the four geometric isomers of 9,11-hexadecadienal (1-4), in addition to hexadecanal and (z)-hexadec-11-enal. we here report the syntheses and characterization of compounds 1-4. one starting material, 9-decen-1-ol, has been used to obtain all of them via divergent synthetic routes. | 2002 | 12088437 |
| comparison of penaeid shrimp and insect parvoviruses suggests that viral transfers may occur between two distantly related arthropod groups. | the dna and putative amino acid sequences of representative insect and shrimp parvoviruses (subfamily densovirinae) were analyzed using computer programs. shrimp viruses included hepatopancreatic parvovirus (hpv) of penaeus monodon (hpvmon) and p. chinensis (hpvchin), spawner-isolated mortality virus from p. monodon (smvmon) and infectious hypodermal and hematopoietic necrosis virus (ihhnv) from p. vannamei. insect viruses included aedes aegypti densovirus (aaednv), aedes albopictus densovirus ( ... | 2002 | 12135792 |
| ultrastructure of the ovariole sheath in diatraea saccharalis (lepidoptera: pyralidae). | the ultrastructure of the ovariole sheath along the diatraea saccharalis ovariole was studied by scanning and transmission electron microscopy. each ovariole is surrounded by an epithelial sheath, a tunica propria and scattered lumen cells. these three components of the ovariole sheath show different ultrastructural features along the ovariole, in the germarium or in the vitellarium; these differences are more evident in the epithelial sheath cells. the epithelial sheath is composed by two layer ... | 2002 | 12240557 |
| electrophysiological studies and identification of possible sex pheromone components of brazilian populations of the sugarcane borer, diatraea saccharalis. | virgin female gland extracts of sugarcane moth diatraea saccharalis (fabricius) (lepidoptera: pyralidae), from three locations in brazil, have been analyzed. by gc-ms analysis and comparison of the chromatographic retention time of the components of the pheromone gland with those retention times of synthetic standards, we observed the presence of (z)-hexadec-11-enal (1), hexadecanal (2), (9e,11z)-hexadecadienal (4), (9z,11z)-hexadecadienal (5) and (9e,11e)-hexadecadienal (6), as minor components ... | 2002 | 12241007 |
| beauveria bassiana yeast phase on agar medium and its pathogenicity against diatraea saccharalis (lepidoptera: crambidae) and tetranychus urticae (acari: tetranychidae). | beauveria bassiana colonizes insect hosts initially through a yeast phase, which is common in some artificial liquid cultures, but not reported on artificial solid media. we describe a yeast-like phase for b. bassiana isolate 447 (atcc 20872) on macconkey agar and its virulence toward diatraea saccharalis and tetranychus urticae. the yeast-like cells of b. bassiana developed by budding from germinating conidia after 24-h incubation. cells were typically 5-10 microm and fungal colonies were initi ... | 2002 | 12445790 |
| purification and characterization of three beta-glycosidases from midgut of the sugar cane borer, diatraea saccharalis. | three beta-glycosidases, named betagly1, betagly2 and betagly3, were isolated from midgut tissues of the sugar cane borer, diatraea saccharalis fabricius (lepidoptera: pyralidae). the three enzymes have similar mr (58,000; 61,000; 61,000), pi (7.5, 7.4, and 7.4) and optimum ph (6.7, 6.3, and 7.2) and were resolved by hydrophobic chromatography. the beta-glycosidases prefer beta-glucosides to beta-galactosides, have four subsites for glucose binding and hydrolyse glucose-glucose beta-1,3 linkages ... | 2003 | 12459203 |
| quantification and development of teratocytes in novel-association host-parasitoid combinations. | we studied the development of teratocytes derived from two congeneric gregarious endoparasitic species, cotesia chilonis and c. flavipes, parasitizing two congeneric novel hosts, diatraea saccharalis and d. grandiosella. the host-parasitoid combinations studied allowed us to investigate relationships between host suitability and teratocyte development. d. saccharalis was a suitable host for both parasitoids, whereas d. grandiosella was suitable for c. chilonis development but often encapsulated ... | 2001 | 12770148 |
| laboratory and field evaluation of beauveria bassiana against sugarcane stalkborers (lepidoptera: pyralidae) in the lower rio grande valley of texas. | of approximately 18,200 ha planted to sugarcane in south texas, only approximately 80 ha (<0.5%) are treated with insecticides because this type of control is widely regarded as ineffective against stalkboring pyralids, the key pests of sugarcane. therefore, nonchemical control measures, such as resistant varieties and biological controls, must be evaluated to mitigate the losses caused by stalkborers. we performed laboratory and field evaluations on the use of beauveria bassiana (balsamo) vuill ... | 2000 | 14658512 |
| competition of bacillus thuringiensis cry1 toxins for midgut binding sites: a basis for the development and management of transgenic tropical maize resistant to several stemborers. | binding and competition of five bacillus thuringiensis toxins--cry1ab, cry1ac, cry1ba, cry1ca, and cry1ea--for midgut binding sites from three pests, spodoptera frugiperda, diatraea saccharalis, and diatraea grandiosella, were investigated as part of a strategy to develop tropical transgenic maize resistant to several stemborers. on s. frugiperda, cry1ab and cry1ac compete for the same binding site; cry1ba and cry1ca compete for a second binding site. cry1ea recognizes a third specific binding s ... | 2004 | 15297925 |
| trypsin inhibitor from poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases. | plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves of being attacked by insects. in this work, a novel trypsin inhibitor (ppti) was purified from the seeds of the native brazilian tree poecilanthe parviflora (benth) (papilioinodeae, leguminosae) by gel filtration chromatography on a sephadex g-100 followed by superdex g75 chromatography (fplc), sepharose 4b-trypsin column, and fractionated by reversed-phase hplc on a c-18 column. sds-pag ... | 2004 | 15328890 |
| [natural presence of beauveria bassiana (balsamo) vuillemin in the sugar cane (saccharum sp. hybrid) in cuba]. | the natural presence of beauveria bassiana was determined (balsamo) vuillemin from insects with mycotic symptoms collected in leaves and in the stalks of sugar cane (saccharum sp. hybrid) in pinar del río, ciudad de la habana, habana, matanzas, villa clara, cienfuegos and camagüey. the results obtained demonstrate the natural presence of the entomopathogen fungus in larvae and chrysalises of diatraea saccharalis (fabricius) and they suggest the basis for the strategy of biological control agains ... | 2004 | 15458363 |
| ultrastructure of the lyonet's glands in larvae of diatraea saccharalis fabricius (lepidoptera: pyralidae). | the lyonet's gland is found in lepidoptera larvae, close to the excretory duct of the silk gland. the role played by this gland is still uncertain. this work aims to describe the ultrastructure of the lyonet's gland in diatraea saccharalis larvae, offering suggestions regarding its possible function. the insects were reared under laboratory-controlled conditions. the glands were conventionally prepared for transmission (tem) and scanning (sem) electron microscopy. sem showed that lyonet's glands ... | 2004 | 15462568 |
| sucrose hydrolases from the midgut of the sugarcane stalk borer diatraea saccharalis. | a beta-fructosidase (ec 3.2.1.26) was isolated from the midgut of larval sugar cane stalk borer diatraea saccharalis by mild-denaturing electrophoresis and further purified to near homogeneity by gel filtration. beta-fructosidase hydrolysed sucrose, raffinose and the fructosyl-trisaccharide isokestose, but it had no activity against maltose, melibiose and synthetic substrates for alpha-glucosidases. two other sucrose hydrolases, one resembling a alpha-glucosidase (ec 3.2.1.20) and the other one ... | 2004 | 15607512 |
| management of feeding damage and survival of southwestern corn borer and sugarcane borer (lepidoptera: crambidae) with bacillus thuringiensis transgenic field corn. | the efficacy of transgenic corn hybrids expressing an insecticidal bacillus thuringiensis (bt) delta-endotoxin from different transformation events was evaluated in field corn, zea mays l., against the southwestern corn borer, diatraea grandiosella dyar, and sugarcane borer, diatraea saccharalis (f.). susceptibilities of neonates and third instars were determined on bt and non-bt corn plants (v6 and r1 stages) in field plots and corn leaf tissue feeding exposure in laboratory bioassays. bt corn ... | 2004 | 15666772 |
| genetic relationships between resistance to stalk-tunneling by the european corn borer and cell-wall components in maize population b73xb52. | the objective of this study was to assess the relationships among quantitative trait loci (qtl) detected for european corn borer (ecb) tunneling and cell-wall components (cwc) neutral detergent fiber (ndf), acid detergent fiber (adf), and acid detergent lignin (adl) content in leaf-sheath and stalk tissues in a maize recombinant inbred line population derived from inbred lines b73 and b52. most of the qtl for ecb resistance (10/13) were at qtl positions for one or more cwc. of the 12 qtl for ndf ... | 2005 | 15887040 |
| reduced susceptibility to tebufenozide in populations of the sugarcane borer (lepidoptera: crambidae) in louisiana. | susceptibility of the sugarcane borer, diatraea saccharalis (f.), to tebufenozide was measured using a feeding bioassay, and values obtained were compared with baselines generated before the use of this insecticide in louisiana sugarcane, saccharum spp. results from our study suggest that susceptibility to tebufenozide is decreasing in field-collected sugarcane borers. inflections in the log dosage-probit lines were detected for many of the field collections, indicating increased heterogeneity w ... | 2005 | 16022328 |
| cowpea (vigna unguiculata) vicilins bind to the peritrophic membrane of larval sugarcane stalk borer (diatraea saccharalis). | in this work, we show that vicilins from two vigna unguiculata (cowpea) genotypes, epace-10 and it 81d-1045, which are susceptible and resistant to attack by the cowpea weevil callosobruchus maculatus, respectively, associate with the peritrophic membrane (pm) from larvae of diatraea saccharalis. solutions with increasing concentrations of vicilins were incubated with pm of the larvae and subsequently analysed by electrophoresis with sds. it was observed that the majority of the bands of approxi ... | 2003 | 16256689 |
| host refractoriness of the tobacco hornworm, manduca sexta, to the braconid endoparasitoid cotesia flavipes. | cotesia flavipes (hymenoptera:braconidae) is a gregarious endoparasitoid of several pyralid stemborer larvae of economic significance including the sugarcane borer, diatraea saccharalis. in this study, the ability of this parasitoid to develop in a sphingid host, manduca sexta, was tested. first, second, third, fourth, and even pharate fifth instar host tobacco hornworm larvae were readily parasitized by the female c. flavipes parasitoids but no wasp larvae hatched from the eggs in this refracto ... | 2005 | 16304618 |
| absorption of toxic beta-glucosides produced by plants and their effect on tissue trehalases from insects. | trehalases present in body wall, malpighian tubules, fat body, midgut and haemolymph from tenebrio molitor (coleoptera), musca domestica (diptera), spodoptera frugiperda and diatraea saccharalis (lepidoptera) were assayed in the presence and absence of toxic beta-glucosides produced by plants or their aglycones. the glucosides used were phlorizin, amygdalin, prunasin and the aglycone mandelonitrile. in addition, t. molitor and s. frugiperda trehalases were assayed with and without esculin. more ... | 2006 | 16458033 |
| a species of alcohol-forming bacteria isolated from the interior of stalks of sugar cane infested with the cane-borer diatraea saccharalis. | 1916 | 16558695 | |
| comparative susceptibility of european corn borer, southwestern corn borer, and sugarcane borer (lepidoptera: crambidae) to cry1ab protein in a commercial bacillus thuringiensis corn hybrid. | one field strain each of the european corn borer, ostrinia nubilalis (hübner); southwestern corn borer, diatraea grandiosella dyar; and sugarcane borer, diatraea saccharalis (f.); were collected from cornfields in northeastern louisiana. susceptibilities of the field strain and a corresponding laboratory strain of the three borer species to cry1ab protein in dk69-70 bacillus thuringiensis (bt) corn hybrid were determined by exposing neonates to intact leaf tissues from whorl stage plants or by f ... | 2006 | 16573340 |
| laboratory and field tests of spray-dried and granular formulations of a bacillus thuringiensis strain with insecticidal activity against the sugarcane borer. | formulations of bacillus thuringiensis berliner (bt) with insecticidal activity against the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: pyralidae), were developed and tested under laboratory and field conditions. the formulations were prepared using biodegradable polymers such as modified corn starch as an encapsulating agent, gelatin as an adherent, powdered sugarcane as a feeding stimulant and a bt var. kurstaki gm-34 strain from a non-sugarcane region as the active ingredien ... | 2006 | 16786544 |
| morphometry of the midgut epithelium of diatraea saccharalis fabricius, 1794 (lepidoptera) parasitized by cotesia flavipes cameron, 1891 (hymenoptera). | the morphometric study of the midgut in diatraea saccharalis (lepidoptera) larvae parasitized by the cotesia flavipes (hymenoptera) showed that there was significant increase in the columnar, goblet and regenerative cells and their nuclei; the midgut lumen diameter and the epithelial height were also increased in the parasitized larvae. the multivariate analysis showed that parasitism affected the columnar cell only in the posterior region, and the goblet cells along the midgut length (anterior ... | 2006 | 16793057 |
| sugarcane borer (lepidoptera: crambidae) management threshold assessment on four sugarcane cultivars. | this research assesses the potential for using different economic injury thresholds in management of a key insect pest on susceptible and resistant commercially produced cultivars of sugarcane (saccharum spp. hybrids). in a 2-yr sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), study involving four commercially produced sugarcane cultivars and four insecticide treatment thresholds, 'lcp 85-384' and 'hocp 91-555' were the most susceptible based on percentage of bored internodes ... | 2006 | 16813338 |
| resistance to stem borers (lepidoptera: crambidae) among texas rice cultivars. | a 4-yr field study was conducted to assess the resistance of rice, oryza sativa l., cultivars to injury from the sugarcane borer, diatraea saccharalis (f.), and the mexican rice borer, eoreuma loftini (dyar) (both lepidoptera: crambidae). several cultivars showed reduced levels of injury ('clxl8', 'xl8', 'wells', 'cheniere', and 'xp710' in 2003; clxl8, xp723, cheniere, and 'cl161' in 2004) and lower stem borer yield loss (clxl8 in 2004) than the more susceptible 'priscilla'. the resistant clxl8 ... | 2006 | 17066824 |
| morphological aspects of cluster formation in the germarium of the sugarcane borer diatraea saccharalis fabricius (lepidoptera: pyralidae). | diatraea saccharalis f. is one of the greatest pests of the sugar cane culture. this report aimed to characterize the germarium region of the sugarcane borer by light and transmission electron microscopy, emphasizing the morphological steps of the ovarian cluster formation. in the germarium of this insect, four zones could be morphologically identified during the cluster formation. in the most apical end of each ovariole--zone i--the germ line stem cells undergo complete mitotic division, origin ... | 2006 | 17144137 |
| sugarcane borer (lepidoptera: crambidae) resistance to transgenic bacillus thuringiensis maize. | transgenic maize, zea mays l., expressing the bacillus thuringiensis (bt) crylab toxin has been planted to extensive areas across the united states and several other countries, but no resistance has been documented in field populations oflepidopteran target pests. this article describes the first report of resistance alleles to commercially available cry1ab bt maize in a louisiana population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). two hundred thirteen two-parent i ... | 2007 | 17370824 |
| mortality staging of dominant lethals induced in the f1 generation of the sugarcane borer; diatraea saccharalis (f.). | comparative egg maturation was observed between fertile eggs laid by unirradiated females, by unirradiated females mated with irradiated males, by irradiated females mated with normal males, and by virgin unirradiated females. haploid eggs turn yellow and dry by the fifth day. eggs from irradiated parents develop more slowly than normal eggs. most embryos from parents irradiated as p generation adults at 16.8 kilorads for males and 12.0 kr for females die early in embryonic development. a descri ... | 1968 | 17387934 |
| interactions between isolates of the entomopathogenic fungus metarhizium anisopliae and the entomopathogenic nematode heterorhabditis bacteriophora jpm4 during infection of the sugar cane borer diatraea saccharalis (lepidoptera: pyralidae). | interactions between the nematode heterorhabditis bacteriophora isolate jpm4 and the fungus metarhizium anisopliae, isolates lpp45 and lpp39, were studied during dual infections of diatraea saccharalis. mortality, production of infective juveniles (ijs) and production of conidia were evaluated. a positive effect was demonstrated for host mortality in duel infections of jpm4 and lpp39, causing 100% mortality with lt(50) and lt(95) values of 1.8 and 2.8 days, respectively. higher values were seen ... | 2007 | 17532003 |
| [artificial diet for rearing doru luteipes (scudder) (dermaptera: forficulidae), a predator of the fall armyworm, spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae)]. | a new technique involving an artificial diet and an artificial substrate for oviposition for the rearing of the predator doru luteipes (scudder) is suggested. both adults and nymphs were maintained in petri dishes containing a transparent piece of soda straw filled with moistened cotton and the corresponding food for the biossays. the following treatments were tested: eggs of spodoptera frugiperda (j.e. smith), diatraea saccharalis fabricius (lepidoptera: pyralidae) and anagasta kuehniella zelle ... | 2007 | 17607467 |
| f2 screen for resistance to a bacillus thuringiensis-maize hybrid in the sugarcane borer (lepidoptera: crambidae). | a novel f2 screening technique was developed for detecting resistance in sugarcane borer, diatraea saccharalis (f.), to transgenic bacillus thuringiensis (bt)-maize expressing the cry1ab insecticidal protein. the f2 screening method involved (i) collecting larvae from maize fields; (ii) establishing two-parent families; (iii) screening f2 neonates for survival on bt-maize leaf tissues; and (iv) confirming resistance on commercial bt-maize plants. with the f2 screening method, 213 iso-line famili ... | 2007 | 17916262 |
| histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer diatraea saccharalis (fabricius) (lepidoptera: crambidae). | the silk gland in lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. the secretion of fibroin and sericin by the different silk gland regions are well established. there are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. this study characterizes the glandular region and detects the presence of lipid components in the secr ... | 2007 | 18060296 |
| ultrastructure of the excretory duct in the silk gland of the sugarcane borer diatraea saccharalis (lepidoptera: pyralidae). | the excretory duct in the silk gland of the sugarcane borer diatraea saccharalis consists of two morphologically distinct regions, recognized by scanning and transmission electron microscopy. the thin posterior region, adjacent to the glandular region, presents a regular surface. secretory vesicles containing either electron-dense or fibrillar cuticular-like materials are observed in their apical cytoplasm; the same cuticular materials were detected as extracellular deposits among the microvilli ... | 2002 | 18088967 |
| evaluation of transgenic bacillus thuringiensis corn hybrids against cry1ab-susceptible and -resistant sugarcane borer (lepidoptera: crambidae). | a louisiana strain of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), was selected for resistance to the crylab protein of bacillus thuringiensis (bt) by using an f2 screening procedure. survival of bt-resistant, -susceptible, and -heterozygous genotypes of sugarcane borer was evaluated on vegetative and reproductive stages of five non-bt and seven bt field corn, zea mays l., hybrids in a greenhouse study. larval survival was recorded 21 d after infestation of neonates o ... | 2007 | 18232406 |
| influence of weather conditions and density of doru luteipes (dermaptera: forficulidae) on diatraea saccharalis (lepidoptera: crambidae) egg mortality. | the influence of weather factors and the predator doru luteipes scudder density on diatraea saccharalis (f.) egg predation was studied. mortality of d. saccharalis eggs was determined by artificially infesting maize plots with egg masses at various times within each of two maize-growing seasons. each egg cohort was monitored every 24-48 h to determine the fate of eggs, and predation rates were calculated. doru luteipes were sampled every 7-10 d, and the mean air temperature, the minimum percenta ... | 2007 | 18284741 |
| comparison of three methods of parasitoid polydnavirus genomic dna isolation to facilitate polydnavirus genomic sequencing. | a major long-term goal of polydnavirus (pdv) genome research is to identify novel virally encoded molecules that may serve as biopesticides to target insect pests that threaten agriculture and human health. as pdv viral replication in cell culture in vitro has not yet been achieved, several thousands of wasps must be dissected to yield enough viral dna from the adult ovaries to carry out pdv genomic sequencing. this study compares three methods of pdv genomic dna isolation for the pdv of cotesia ... | 2008 | 18348210 |
| effects of soybean proteinase inhibitors on development of the soil mite scheloribates praeincisus (acari: oribatida). | proteinase inhibitors (pi) are present in plant tissues, especially in seeds, and act as a defense mechanism against herbivores and pathogens. serine pi from soybean such as bowman-birk (bbpi) and kunitz have been used to enhance resistance of sugarcane varieties to the sugarcane borer diatraea saccharalis (fabricius) (lepidoptera: crambidae), the major pest of this crop. the use of these genetically-modified plants (gm) expressing pi requires knowledge of its sustainability and environmental sa ... | 2008 | 18357504 |
| effects of entomopathogenic bacterium photorhabdus temperata infection on the intestinal microbiota of the sugarcane stalk borer diatraea saccharalis (lepidoptera: crambidae). | photorhabdus temperata is an entomopathogenic bacterium that is associated with nematodes of the heterorhabditidae family in a symbiotic relationship. this study investigated the effects of p. temperata infection on the intestinal microbiota of the sugarcane stalk borer diatraea saccharalis. histopathology of the infection was also investigated using scanning electron microscopy. groups of 20 larvae were infected by injection of approximately 50 bacterial cells directly into the hemocoel. after ... | 2008 | 18406423 |
| allele frequency of resistance to bacillus thuringiensis cry1ab corn in louisiana populations of sugarcane borer (lepidoptera: crambidae). | transgenic bacillus thuringiensis (bt) corn, zea mays l., has been widely used to manage a corn borer complex in the mid-southern region of the united states. the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), has become a dominant cornstalk boring species in some areas of this region, especially in louisiana. therefore, management of sugarcane borer resistance to bt corn is critical to ensure the long-term sustainability of bt corn for the region. this study screened 280 t ... | 2008 | 18459416 |
| tolerance and compensatory response of rice to sugarcane borer (lepidoptera: crambidae) injury. | a 3-yr field experiment was conducted to evaluate the tolerance and compensatory response of rice (oryza sativa l.) to injury caused by sugarcane borer, diatraea saccharalis (f.), as affected by cultivar (cocodrie, francis, and jefferson), stage of crop growth during which the injury occurred (third tiller stage, panicle differentiation stage, and heading stage), and sugarcane borer density. the proportion of rice tillers with sugarcane borer injury (leaf and leaf sheath injury and/or stem injur ... | 2008 | 18559187 |
| spherites in the midgut epithelial cells of the sugarcane borer parasitized by cotesia flavipes. | diatraea saccharalis, the main pest of sugarcane, has been controlled by cotesia flavipes. very little is known about the effect of parasitism on the host organs, including the midgut. the lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. spherites have been described in columnar and regenerative cells of several lepidoptera species, and presented a lot of functional meaning. we identified spherites in the midgut epithelial cells of non-parasitized ... | 2008 | 18669324 |
| dna barcode information for the sugar cane moth borer diatraea saccharalis. | we reviewed the use and relevance of barcodes for insect studies and investigated the barcode sequence of diatraea saccharalis. this sequence has a high level of homology (99%) with the barcode sequence of the crambidae (lepidoptera). the sequence data can be used to construct relationships between species, allowing a multidisciplinary approach for taxonomy, which includes morphological, molecular and distribution data, all of which are essential for the understanding of biodiversity. the d. sac ... | 2008 | 18767242 |
| effects of entomopathogenic bacterium photorhabdus temperata infection on the digestive enzymes of diatraea saccharalis (lepidoptera: crambidae) larvae. | this study investigated the effects of photorhabdus temperata infection on the activities of digestive enzymes of the sugarcane stalk borer diatraea saccharalis. non-infected d. saccharalis larvae present a major alpha-amylase, several proteinases, three sucrose hydrolases and two alpha-glucosidases in their midgut. analysis of these hydrolases by electrophoresis and "in gel" assays showed that the activities of all enzymes decreased following infection, with an initial decline observed 12 h aft ... | 2008 | 18782059 |
| isobutyl amides--potent compounds for controlling diatraea saccharalis. | a dichloromethane-methanol extract of the seeds of piper tuberculatum jacq. (piperaceae) and two isobutyl amides, 4,5-dihydropiperlonguminine (1) and pellitorine (2), which were isolated by chromatographic methods, were assayed for their lethality against the sugarcane borer diatraea saccharalis f. (lepidoptera: pyralidae). | 2009 | 18785219 |
| trypsin inhibitors in passion fruit (passiflora f. edulis flavicarpa) leaves: accumulation in response to methyl jasmonate, mechanical wounding, and herbivory. | this work investigates the effect of methyl jasmonte (meja), mechanical wounding, and herbivory caused by larval feeding of a specialist insect ( agraulis vanillae vanillae) upon trypsin inhibitory activity in passion fruit leaves. despite the fact that all treatments caused accumulation of trypsin inhibitors (tis), higher levels were observed in meja treated leaves when plants were assayed 24 and 48 h after stimulus. concerning both mechanically injured plants and attacked ones, a systemic indu ... | 2008 | 18795783 |
| morphological regional differences of epithelial cells along the midgut in diatraea saccharalis fabricius (lepidoptera: crambidae) larvae. | the sugarcane borer, diatraea saccharalis fabricius, is a pest to sugarcane and many other crops. this work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of d. saccharalis larvae. fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. there are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depend ... | 2016 | 18813743 |
| susceptibility of cry1ab-resistant and -susceptible sugarcane borer (lepidoptera: crambidae) to four bacillus thuringiensis toxins. | sugarcane borer, diatraea saccharalis (f.), is a primary corn stalk borer pest targeted by transgenic corn expressing bacillus thuringiensis (bt) proteins in many areas of the mid-southern region of the united states. recently, genes encoding for cry1a.105 and cry2ab2 bt proteins were transferred into corn plants (event mon 89034) for controlling lepidopteran pests. this new generation of bt corn with stacked-genes of cry1a.105 and cry2ab2 will become commercially available in 2009. susceptibili ... | 2009 | 18955062 |
| purification and characterization of a trypsin inhibitor from plathymenia foliolosa seeds. | a novel trypsin inhibitor (pfti) was isolated from plathymenia foliolosa (benth.) seeds by gel filtration chromatography on a sephadex g-100, deae-sepharose, and trypsin-sepharose columns. by sdspage, pfti yielded a single band with a m(r) of 19 kda. pfti inhibited bovine trypsin and bovine chymotrypsin with equilibrium dissociation constants (k(i)) of 4 x 10(-8) and 1.4 x 10(-6) m, respectively. pfti retained more than 50% of activity at up to 50 degrees c for 30 min, but there were 80 and 100% ... | 2008 | 18991455 |
| selection and life history traits of tebufenozide-resistant sugarcane borer (lepidoptera: crambidae). | varying susceptibility to tebufenozide was recorded in the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), collected from louisiana sugarcane locations with different selection pressures. results from diet incorporation bioassays with tebufenozide indicated significant increases in lc50 (3.78-fold) and lc90 (7-fold) values for a colony from duson (du), an area with higher selection pressure, compared with a colony from alexandria (alex), an area with no selection pressure. d ... | 2008 | 19133473 |
| inheritance of resistance to bacillus thuringiensis cry1ab protein in the sugarcane borer (lepidoptera: crambidae). | inheritance traits of a cry1ab-resistant strain of the sugarcane borer, diatraea saccharalis (f.) were analyzed using various genetic crosses. reciprocal parental crosses between cry1ab-susceptible and cry1ab-resistant populations, f(1) by f(1) crosses, and backcrosses of f(1) with the cry1ab-resistant population were successfully completed. larval mortality of the parental and cross-populations were assayed on cry1ab diet and bacillus thuringiensis (bt)-corn leaf tissue. maternal effects and se ... | 2009 | 19527726 |
| impact of hurricane rita storm surge on sugarcane borer (lepidoptera: crambidae) management in louisiana. | twelve thousand to 16,000 ha of louisiana sugarcane (saccharum spp.) fields were flooded by saltwater from the hurricane rita storm surge in september 2005. a four treatment, 12-replication study comparing storm surge flooded and nonflooded plant and ratoon sugarcane fields was conducted during summer 2006 to assess sugarcane borer, diatraea saccharalis (f.), pest severity, pest control actions, and soil-associated arthropod abundance and diversity. even with a significant 2.4-fold increase in t ... | 2009 | 19610419 |
| toxicity of hydrolyzed vicilins toward callosobruchus maculatus and phytopathogenic fungi. | studies have shown that vicilins (7s storage proteins) from seeds were able to bind to the surface of the callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of diatraea saccharalis and tenebrio molitor , inhibiting larval development. vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. the present work studies the association of peptides from vicilins of genotypes of vigna unguicula ... | 2009 | 19658384 |
| mitochondria in the midgut epithelial cells of sugarcane borer parasitized by cotesia flavipes (cameron, 1891). | the sugarcane borer diatraea saccharalis (lepidoptera: crambidae) has been controlled by cotesia flavipes (hymenoptera: braconidae); however, very little is known about the effect of the parasitism in the host organs, including the midgut. this work aims to verify mitochondrial alteration in the different midgut epithelial cells of d. saccharalis parasitized by c. flavipes. midgut fragments (anterior and posterior region) of both non-parasitized and parasitized larvae were processed for transmis ... | 2010 | 20231974 |
| rice tillering and yield as affected by artificial and sugarcane borer (lepidoptera: crambidae) culm injury. | a 2-yr study was conducted to evaluate the tillering and yield response of rice, oryza sativa l., whose culms were injured artificially or by larval sugarcane borers, diatraea saccharalis (f.). artificially injured plants produced approximately 0.49 more tillers than uninjured plants, similar to what has previously been reported for larval injured plants. in contrast, artificial injury did not affect yield per tiller, whereas larval injury did. the proximity of larval injury to the panicle had a ... | 2010 | 20388284 |
| inorganic elements in the fat bodies of diatraea saccharalis (lepidoptera: crambidae) larvae parasitized by cotesia flavipes (hymenoptera: braconidae). | koinobiont parasitoids use several strategies to regulate the host's physiological processes during parasitism. although many aspects of host-parasitoid interactions have been explored, studies that attempted to assess the effects of parasitism on the availability of inorganic elements in the host are virtually nonexistent. therefore, we aimed to evaluate the effects of parasitism on the concentrations of inorganic elements in the fat bodies of larvae of diatraea saccharalis (lepidoptera: crambi ... | 2010 | 20388551 |
| induction of a gloverin-like antimicrobial polypeptide in the sugarcane borer diatraea saccharalis challenged by septic injury. | diatraea saccharalis (fabricius, 1794) (lepidoptera: crambidae) is an important pest for brazilian sugarcane. in the present study, we detected two distinct spots in hemolymph from septic injured larvae (hds1 and hds2), which are separated by 2de gel electrophoresis. both spots were subjected to in-gel tryptic digestion and maldi-tof/tof analysis, which revealed the sequence vfgtlgsddsglfgk present in both hds1 and hds2. this sequence had homology and 80% identity with specific lepidoptera antim ... | 2010 | 20490430 |
| [effects of adult feeding on the reproduction and longevity of noctuidae, crambidae, tortricidae and elachistidae species]. | this research evaluates the effect of the adult diet on the reproduction of anticarsia gemmatalis hübner, heliothis virescens (fabr.), spodoptera frugiperda (j. e. smith) (noctuidae), diatraea saccharalis (fabr.) (crambidae), gymnandrosoma aurantianum lima (tortricidae) and stenoma catenifer walsingham (elachistidae). adults of all species were fed either water or a 10% honey solution. the egg viability for the 1st and 2nd egg masses, adult fecundity, longevity, number of mating and the ovigeny ... | 2010 | 20498952 |
| geographic variation of sex pheromone and mitochondrial dna in diatraea saccharalis (fab., 1794) (lepidoptera: crambidae). | (9z,11e)-hexadecadienal and (z11)-hexadecenal, the main sex pheromone components of the sugarcane borer, diatraea saccharalis, were identified and quantified from four brazilian and one colombian populations using gc-ead, gc-ms and gc analyses. three different ratios were observed, 9:1, 6:1, and 3:1. the pheromone concentration for the major component, (9z,11e)-hexadecadienal, varied from 6.8 ng/gland to 21.9 ng/gland and from 1.7 ng/gland to 6.5 to the minor component, (z11)-hexadecenal. the 25 ... | 2010 | 20558173 |
| deleterious activity of natural products on postures of spodoptera frugiperda (lepidoptera: noctuidae) and diatraea saccharalis (lepidoptera: pyralidae). | the control of lepidoptera pests should be carried out before hatching of their caterpillars to avoid damage to the crops. the aim of this work was to assess the activity of neem (trade name: natuneem, producer: base fértil, chapadão do sul, brazil) and pyroligneous extracts (trade name: biopirol 7m, producer: biocarbo, itabirito, brazil) at 10 ml/l (1%) and 20 ml/l (2%) contents on egg masses of different ages of spodoptera frugiperda smith (lepidoptera: noctuidae) and of diatraea saccharalis f ... | 2010 | 20653245 |
| molecular characterization and rna interference of three midgut aminopeptidase n isozymes from bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, diatraea saccharalis. | aminopeptidase n (apn) proteins located at the midgut epithelium of some lepidopteran species have been implicated as receptors for insecticidal proteins from bacillus thuringiensis. cdnas of three apn isoforms, dsapn1, dsapn2, and dsapn3, from cry1ab-susceptible (cry1ab-ss) and -resistant (cry1ab-rr) strains of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), were identified and sequenced using reverse transcriptase polymerase chain reaction (rt-pcr) and 5' rapid amplifi ... | 2010 | 20685334 |
| structural characteristics and phylogenetic analysis of the mitochondrial genome of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is an economically important pest of several major crops in north and south america. there is great concern over potential resistance development to the valuable transgenic bacillus thuringiensis corn. cost-effective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field populations of d. saccharalis, are needed. this article reports the complete mitochondrial genome sequen ... | 2011 | 20849253 |
| analysis of nucleus activity in malpighian tubules of diatraea saccharalis (fabricius) (lepidoptera: crambidae) larvae by critical electrolyte concentration. | diatraea saccharalis (fabricius) has cryptonephridial type malphigian tubules (mt). this type of mt is characterized by the penetration of the distal part of the mt into the external walls of the rectum, which is usually lined with a perinephral membrane. the mt is divided into three differentiated regions: proximal, middle and distal. in this study, our objective was to compare the nuclear activities of each one of the three regions of the d. saccharalis mt by using a nuclear basophilic techniq ... | 2010 | 20877993 |
| genetic diversity analysis with rapd linked to sex identification in the sugar cane borer diatraea saccharalis. | diatraea saccharalis is an insect that causes considerable losses in the sugar cane crop. our aim was to contribute to the knowledge of the biology of d. saccharalis, with the report of dna fragments involved in the differentiation between the male and female of this species using the rapd sex molecular marker gyakuu-13, which is specific for the w chromosome of bombyx mori. another point evaluated in this study was the genetic diversity of a d. saccharalis population maintained by inbreeding in ... | 2010 | 21128215 |
| f(2) screening for resistance to pyramided bacillus thuringiensis maize in louisiana and mississippi populations of diatraea saccharalis (lepidoptera: crambidae). | background: in the mid-southern region of the united states, sugarcane borer, diatraea saccharalis (f.), is a major target pest of transgenic maize expressing bacillus thuringiensis (bt) proteins. novel transgenic maize technologies containing two or more pyramided bt genes for controlling lepidopteran pests have recently become commercially available. insect resistance management (irm) is an important issue in the sustainable use of bt crop technologies. the objective of this study was to deter ... | 2011 | 21538799 |
| population dynamics of caterpillars on three cover crops before sowing cotton in mato grosso (brazil). | direct seeding mulch-based cropping systems under a preliminary cover crop such as millet are common in some areas of brazil. lepidopteran pests that damage cotton, soybean and maize crops can proliferate on cover crops, so preventive chemical treatments are necessary. very little data is available on these pests on cover crops. this paper presents the dynamics of spodoptera frugiperda, s. eridania, mocis latipes and diatraea saccharalis caterpillars monitored at primavera do leste, mato grosso ... | 2010 | 21539250 |
| structural insights regarding an insecticidal talisia esculenta protein and its biotechnological potential for diatraea saccharalis larval control. | talisin is a seed-storage protein from talisia esculenta that presents lectin-like activities, as well as proteinase-inhibitor properties. the present study aims to provide new in vitro and in silico biochemical information about this protein, shedding some light on its mechanistic inhibitory strategies. a theoretical three-dimensional structure of talisin bound to trypsin was constructed in order to determine the relative interaction mode. since the structure of non-competitive inhibition has n ... | 2012 | 21983187 |
| down regulation of a gene for cadherin, but not alkaline phosphatase, associated with cry1ab resistance in the sugarcane borer diatraea saccharalis. | the sugarcane borer, diatraea saccharalis, is a major target pest of transgenic corn expressing bacillus thuringiensis (bt) proteins (i.e., cry1ab) in south america and the mid-southern region of the united states. evolution of insecticide resistance in such target pests is a major threat to the durability of transgenic bt crops. understanding the pests' resistance mechanisms will facilitate development of effective strategies for delaying or countering resistance. alterations in expression of c ... | 2011 | 21991350 |
| Identification and Structural Characterization of Novel Cyclotide with Activity against an Insect Pest of Sugar Cane. | Cyclotides are a family of plant-derived cyclic peptides comprising six conserved cysteine residues connected by three intermolecular disulfide bonds that form a knotted structure known as a cyclic cystine knot (CCK). This structural motif is responsible for the pronounced stability of cyclotides against chemical, thermal, or proteolytic degradation and has sparked growing interest in this family of peptides. Here, we isolated and characterized a novel cyclotide from Palicourea rigida (Rubiaceae ... | 2012 | 22074926 |
| life table studies of elasmopalpus lignosellus (lepidoptera: pyralidae) on sugarcane. | the lesser cornstalk borer, elasmopalpus lignosellus (zeller) (lepidoptera: pyralidae) is an important pest of sugarcane (a complex hybrid of saccharum spp.) in southern florida. reproductive and life table parameters for e. lignosellus were examined at nine constant temperatures from 13 to 36 °c with sugarcane as the larval food source. the pre- and postoviposition periods decreased with increasing temperatures and reached their minimums at 33 and 36 °c, respectively. the oviposition period was ... | 2010 | 22182570 |
| exploitation of mitochondrial nad6 as a complementary marker for studying population variability in lepidoptera. | the applicability of mitochondrial nad6 sequences to studies of dna and population variability in lepidoptera was tested in four species of economically important moths and one of wild butterflies. the genetic information so obtained was compared to that of cox1 sequences for two species of lepidoptera. nad6 primers appropriately amplified all the tested dna targets, the generated data proving to be as informative and suitable in recovering population structures as that of cox1. the proposal is ... | 2011 | 22215980 |
| parasitism by the endoparasitoid, cotesia flavipes induces cellular immunosuppression and enhances susceptibility of the sugar cane borer, diatraea saccharalis to bacillus thuringiensis. | cotesia flavipes cameron (hymenoptera: braconidae), is a gregarious larval endoparasitoid of the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: crambidae). the aim of this research was to analyze cellular immunosuppression of d. saccharalis parasitized by c. flavipes in terms of encapsulation, melanization, and hemocyte nodule formation. the encapsulation assay was done 1 and 6 days after parasitoid oviposition. in addition, the susceptibility of parasitized and nonparasitzed larv ... | 2011 | 22225507 |
| microarray analysis of global gene regulation in the cry1ab-resistant and cry1ab-susceptible strains of diatraea saccharalis. | extensive adoption of transgenic bt corn in recent years for stalk borer control has increased risk of resistance evolution in the target pest populations. a bt-resistant strain of the sugarcane borer, diatraea saccharalis, was approximately 100-fold more tolerant to cry1ab toxin than the susceptible counterpart. to gain a better understanding of the molecular mechanisms of bt resistance, the cry1ab-susceptible (cry1ab-ss) and cry1ab-resistant (cry1ab-rr) strains of d. saccharalis were subjected ... | 2012 | 22228544 |
| sugarwin: a sugarcane insect-induced gene with antipathogenic activity. | in sugarcane fields, colonization of the stalk by opportunistic fungi usually occurs after the caterpillar diatraea saccharalis attacks the sugarcane plant. plants respond to insect attack by inducing and accumulating a large set of defense proteins. two homologues of a barley wound-inducible protein (barwin), sugarcane wound-inducible proteins sugarwin1 and sugarwin2, have been identified in sugarcane by an in silico analysis. antifungal properties have been described for a number of barwin hom ... | 2012 | 22250584 |
| seasonal infestations of two stem borers (lepidoptera: crambidae) in noncrop grasses of gulf coast rice agroecosystems. | infestations of two stem borers, eoreuma loftini (dyar) and diatraea saccharalis (f.) (lepidoptera: crambidae), were compared in noncrop grasses adjacent to rice (oryza sativa l.) fields. three farms in the texas rice gulf coast production area were surveyed every 6-8 wk between 2007 and 2009 using quadrat sampling along transects. although d. saccharalis densities were relatively low, e. loftini average densities ranged from 0.3 to 5.7 immatures per m(2) throughout the 2-yr period. early annual ... | 2011 | 22251716 |
| feasibility of using an alternative larval host and host plants to establish cotesia flavipes (hymenoptera: braconidae) in the temperate louisiana sugarcane ecosystem. | attempts to establish cotesia flavipes cameron (hymenoptera: braconidae) in louisiana sugarcane fields to control the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae) have been unsuccessful. experiments were conducted to investigate the feasibility of using an alternative larval host and host plants to overcome barriers preventing establishment. in addition, we evaluated c. flavipes' ability to search for d. saccharalis in sugarcane without above-ground internodes. diatraea ev ... | 2012 | 22506999 |
| the nearctic-caribbean species leptotrachelus dorsalis (fabricius, 1801): larval descriptions with a diagnosis of immature ctenodactylini and natural history notes on the genus and tribe (coleoptera, carabidae). | adults and larvae of leptotrachelus dorsalis (fabricius), the sugarcane savior beetle, live in association with grasses, the larvae in the appressed leaf axils. both adult and larval leptotrachelus dorsalis eat larvae of the sugarcane borer, diatraea saccharalis (fabricius), and perhaps other insects living in the confines of the leaf sheaths of that and other grass-like species. the geographic range of leptotrachelus dorsalis extends from kansas in the west to the atlantic seaboard, north as fa ... | 2012 | 22679382 |
| extended monitoring of resistance to bacillus thuringiensis cry1ab maize in diatraea saccharalis (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is a major target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. during 2007-2009, a total of 986 feral individuals of d. saccharalis were collected from maize fields in six locations of louisiana and mississippi and examined for resistance to cry1ab maize using f 1/f 2 screens. major resistance alleles to cry1ab maize in the populations sampled from non-bt maiz ... | 2014 | 22688686 |
| oviposition behavior of diatraea saccharalis (lepidoptera: crambidae) on different rice cultivars in louisiana. | the sugarcane borer, diatraea saccharalis (f.), is a pest of a variety of graminaceous crops in the southern united states, including sugarcane, maize, and rice in louisiana. this study examined several aspects of d. saccharalis oviposition behavior on rice (oryza sativa). the vertical distribution of egg masses on four phenological stages of rice in the field showed that d. saccharalis prefers to oviposit on the uppermost portions of rice plants, regardless of plant stage. in greenhouse choice ... | 2012 | 22732615 |
| effects of neem oil (azadirachta indica a. juss) on midgut cells of predatory larvae ceraeochrysa claveri (navás, 1911) (neuroptera: chrysopidae). | the effects of ingested neem oil, a botanical insecticide obtained from the seeds of the neem tree, azadirachta indica, on the midgut cells of predatory larvae ceraeochrysa claveri were analyzed. c. claveri were fed on eggs of diatraea saccharalis treated with neem oil at a concentration of 0.5%, 1% and 2% during throughout the larval period. light and electron microscopy showed severe damages in columnar cells, which had many cytoplasmic protrusions, clustering and ruptured of the microvilli, s ... | 2013 | 22739123 |
| evaluation of in vitro and in vivo effects of semipurified proteinase inhibitors from theobroma seeds on midgut protease activity of lepidopteran pest insects. | we have characterized in vitro and in vivo effects of trypsin inhibitors from theobroma seeds on the activity of trypsin- and chymotrypsin-like proteins from lepidopteran pest insects. the action of semipurified trypsin inhibitors from theobroma was evaluated by the inhibition of bovine trypsin and chymotrypsin activities determined by the hydrolysis of n-benzoyl-dl-arginine-p-nitroanilide (bapa) and n-succinyl-ala-ala-pho-phe p-nitroanilide (s-(ala)2prophe-pna). proteinase inhibitor activities ... | 2012 | 22806759 |
| molecular cloning and insecticidal effect of inga laurina trypsin inhibitor on diatraea saccharalis and heliothis virescens. | native inga laurina (fabaceae) trypsin inhibitor (ilti) was tested for anti-insect activity against diatraea saccharalis and heliothis virescens larvae. the addition of 0.1% ilti to the diet of d. saccharalis did not alter larval survival but decreased larval weight by 51%. the h. virescens larvae that were fed a diet containing 0.5% ilti showed an 84% decrease in weight. ilti was not digested by the midgut proteinases of either species of larvae. the trypsin levels were reduced by 55.3% in the ... | 2012 | 22885277 |
| evaluation of corn hybrids expressing cry1f, cry1a.105, cry2ab2, cry34ab1/cry35ab1, and cry3bb1 against southern united states insect pests. | studies were conducted across the southern united states to characterize the efficacy of multiple bacillus thuringiensis (bt) events in a field corn, zea mays l., hybrid for control of common lepidopteran and coleopteran pests. cry1f protein in event tc1507 and cry1a.105 + cry2ab2 proteins in event mon 89034 were evaluated against pests infesting corn on above-ground plant tissue including foliage, stalks, and ears. cry34ab1/cry35ab1 proteins in event das-59122-7 and cry3bb1 in event mon 88017 w ... | 2012 | 23156183 |
| susceptibility of cry1ab maize-resistant and -susceptible strains of sugarcane borer (lepidoptera: crambidae) to four individual cry proteins. | sugarcane borer, diatraea saccharalis (f.), is a major target of bt maize in south america and many areas of the us mid-south region. six laboratory strains of d. saccharalis were established from six single-pair f(2) families possessing major resistance alleles to cry1ab maize hybrids. susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated bt proteins, cry1ab, cry1aa, cry1ac and cry1f. bt susceptibility of the six strains was compared with t ... | 2013 | 23270874 |
| production of 3-nitropropionic acid by endophytic fungus phomopsis longicolla isolated from trichilia elegans a. juss ssp. elegans and evaluation of biological activity. | the compound 3-nitropropionic acid is a potent neurotoxic agent in animals and well-known as a potent inhibitor of mycobacterium tuberculosis. in this research, we were able to extract this compound from the endophytic fungus, phomopsis longicolla (fj62759), isolated from trichilia elegans a. juss ssp. elegans. the aim of this study was the isolation of secondary metabolites produced by p. longicolla, the chemical identification of these compounds and evaluation of their antimicrobial and insect ... | 2013 | 23296917 |
| occurrence and larval movement of diatraea saccharalis (lepidoptera: crambidae) in seed mixes of non-bt and bt pyramid corn. | larval movement of target pest populations among bt and non-bt plants is a major concern in the use of a seed mixture refuge strategy for bt resistance management. in this study, occurrence and larval movement of the sugarcane borer, diatraea saccharalis (f.), were evaluated in four planting patterns of non-bt and bt plants containing genuity® smartstax(tm) traits in 2009-2011. the four planting patterns were: (1) a pure stand of 27 bt plants; (2) one non-bt plant in the center, surrounded by 26 ... | 2013 | 23456950 |
| development and characterization of microsatellite loci for genetic studies of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | we present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, diatraea saccharalis (fabricius). we isolated 16 microsatellite loci through an enriched genomic library protocol. after characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on average) and in the polymorphism information content (ranging from 0.292 to 0.771; 0.535 on average). these markers can be used in further studies to ... | 2013 | 23765969 |
| effect of silicon soil amendment on performance of sugarcane borer, diatraea saccharalis (lepidoptera: crambidae) on rice. | the sugarcane borer, diatraea saccharalis (f.), is a pest of graminaceous crops in the southern usa, including sugarcane, maize, and rice. this study was conducted to investigate the effect of silicon (si) soil amendments on performance of sugarcane borer, d. saccharalis, on two rice cultivars, cocodrie and xl723. there was a significant increase in the si content of rice plants supplemented with calcium silicate as compared to non-treated plants. soil si amendment led to lower relative growth r ... | 2013 | 23830057 |
| biological aspects of eriopis connexa (germar) (coleoptera: coccinellidae) fed on different insect pests of maize (zea mays l.) and sorghum [sorghum bicolor l. (moench.)]. | eriopis connexa (germar) (coleoptera: coccinellidae) occurs in several countries of south america and its mass rearing is important for biological control programmes. this work evaluated biological aspects of e. connexa larva fed on eggs of anagasta kuehniella (zeller) (lepidoptera: pyralidae) and spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae) frozen for one day, fresh eggs of diatraea saccharalis (fabricius) (lepidoptera: pyralidae), s. frugiperda newly-hatched caterpillars, nymph ... | 2013 | 23917572 |
| characterization and transcriptional analyses of cdnas encoding three trypsin- and chymotrypsin-like proteinases in cry1ab-susceptible and cry1ab-resistant strains of sugarcane borer, diatraea saccharalis. | diatraea saccharalis is a major corn borer pest. midgut serine proteinases are essential for insect growth and development. alteration of midgut proteinases is responsible for bt resistance development in some species. to clone midgut trypsin and chymotrypsin cdnas and to test if the cry1ab resistance in d. saccharalis is associated with changes in midgut proteinases, total midgut tryptic and chymotryptic activities, cdna sequences, and gene expressions of three trypsin and three chymotrypsin ge ... | 2013 | 23955944 |
| comparative binding of cry1ab and cry1f bacillus thuringiensis toxins to brush border membrane proteins from ostrinia nubilalis, ostrinia furnacalis and diatraea saccharalis (lepidoptera: crambidae) midgut tissue. | the european (ostrinia nubilalis hübner) and asian corn borers (ostrinia furnacalis guenée) are closely related and display similar sensitivity to cry1 toxins. in this study, we compared the binding patterns of cry1ab and cry1f toxins between both ostrinia spp., as well as the expression of putative cadherin- and aminopeptidase-n (apn)-like protein receptors. additionally, cdna sequences of these putative toxin receptors from both ostrinia species were compared. ligand blots for both species ind ... | 2013 | 23999243 |
| bacillus thuringiensis monogenic strains: screening and interactions with insecticides used against rice pests. | the screening of bacillus thuringiensis (bt) cry proteins with high potential to control insect pests has been the goal of numerous research groups. in this study, we evaluated six monogenic bt strains (bt dendrolimus hd-37, bt kurstaki hd-1, bt kurstaki hd-73, bt thuringiensis 4412, bt kurstaki nrd-12 and bt entomocidus 60.5, which codify the cry1aa, cry1ab, cry1ac, cry1ba, cry1c, cry2a genes respectively) as potential insecticides for the most important insect pests of irrigated rice: spodopte ... | 2012 | 24031872 |
| agricultural case studies of classification accuracy, spectral resolution, and model over-fitting. | this paper describes the relationship between spectral resolution and classification accuracy in analyses of hyperspectral imaging data acquired from crop leaves. the main scope is to discuss and reduce the risk of model over-fitting. over-fitting of a classification model occurs when too many and/or irrelevant model terms are included (i.e., a large number of spectral bands), and it may lead to low robustness/repeatability when the classification model is applied to independent validation data. ... | 2013 | 24160886 |
| short and long-term antinutritional effect of the trypsin inhibitor apti for biological control of sugarcane borer. | plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. in this work, we tested the anti-insect activity of adenanthera pavonina trypsin inhibitor (apti) against diatraea saccharalis larvae, a major insect pest in sugarcane. the addition of 0.1% apti in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. apti was not digested after 60h incubation with ... | 2014 | 24355140 |
| fitness costs and stability of cry1ab resistance in sugarcane borer, diatraea saccharalis (f.). | the sugarcane borer, diatraea saccharalis (f.), is a major target species of transgenic corn expressing bacillus thuringiensis (bt) proteins in south america and the u.s. mid-south region. in this study, the fitness of seven insect genotypes of d. saccharalis were assayed on non-toxic diet, which included a cry1ab-susceptible strain (ss-2009), two cry1ab-resistant strains (rr-43a(bc), rr-l5b(bc)), and four f1 hybrids (f1-r43a(m)s(f), f1-r43a(f)s(m), f1-r5b(m)s(f), and f1-r5b(f)s(m)). the f1 hybr ... | 2014 | 24503242 |