Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| linkage analysis of the nuclear homologues of mitochondrial plasmid-like dnas in rice. | a genetical study on the nucleotide sequences of the nuclear dnas which share homology with rice mitochondrial plasmid-like dnas, b1, b2, b3 and b4 was carried out. restriction fragments of the nuclear dnas hybridized with these plasmid-like dnas showed polymorphisms in their length between indica and japonica rice cultivars. the hybridized signals found specifically in indica or japonica cultivars segregated in the f2 population derived from a cross between these two subspecies. the observed ra ... | 1991 | 1685659 |
| analysis of nuclear sequences homologous to the b4 plasmid-like dna of rice mitochondria; evidence for sequence transfer from mitochondria to nuclei. | nuclear sequences homologous to the plasmid-like dna, b4, were analyzed in the japonica rice variety, fujiminori. homologous sequences existed at several positions in the nuclear genome, but each contained only a portion of the b4 sequence. it was impossible to reconstruct the entire sequence of b4 even by collating all the homologous sequences. overlaps between some of the b4 sequences present in the nuclear genome resulted in parts of the sequence being represented more than once. these featur ... | 1991 | 1782676 |
| tritium concentration in japanese rice. | polished rice samples harvested in 1985 were collected from 25 prefectures throughout japan. concentrations of both tissue free water tritium (tfwt) and organically bound tritium (obt) were determined. nearly uniform distribution of the tfwt and obt concentrations was observed in japan, taking into account a relatively large counting error. the average values for all japanese polished rice were 2.69 +/- 0.74 bq/l for the tfwt concentration, 2.01 +/- 0.78 bq/l for the obt concentration and 0.83 + ... | 1990 | 2098551 |
| the relationship between translucency of rice grain and gelatinization of starch in the grain during cooking. | the soaked and nonsoaked rice grains were cooked by the excess water method and the steamer method, and subjected to ranghino's test, x-ray diffraction, and microscopic observation. the starch granules in the nonsoaked rice were gelatinized at the same time as the grains became translucent during cooking. however, when the grains of medium amylose varieties and waxy rice were presoaked, the starch granules were not fully gelatinized in the translucent grains cooked for ranghino's cooking time. t ... | 1987 | 3437340 |
| phytotoxicity tests with the molluscicide n-tritylmorpholine. | n-tritylmorpholine (frescon, wl 8008) has been applied as an emulsifiable concentrate (fx 28) to cotton and rice in glasshouse experiments without any adverse effects. the compound was applied in the irrigation water at 5 and 10 times the rates used for snail control. the experiment on cotton was terminated after 60 days, when the plants became pot-bound. the treatments did not affect the leaf area or the fresh weight of plants. the rice (oryza sativa ssp. japonica) was harvested and no differen ... | 1967 | 5300052 |
| 241am and plutonium in japanese rice-field surface soils. | 1983 | 6663540 | |
| individual differences in blood and breath acetaldehyde levels and urinary excretion of catecholamines after alcohol intake. | sixty three male japanese, aged 20-40 yr were evaluated as to the degree of facial flushing following a controlled dose of ethanol either as japanese rice wine or ethanol 0.4 g kg body weight. thirty four subjects responded with overt facial flushing. the acetaldehyde levels in blood and expired air were significantly higher in the flushing group without a change in ethanol elimination rate. urinary excretion of vanilmandelic acid (vma) and 3 methyoxy-4-hydroxyphenylglycol (mhpg) are reported. | 1980 | 7004232 |
| marchiafava-bignami disease, striatal degeneration, and other neurological complications of chronic alcoholism in a japanese. | a japanese man with a variety of neurological complications, had drunk japanese rice wine (sake) daily for about 25 years. there was a progressive development of parkinsonism, cerebellar ataxia, and mental deterioration by the time he was 32. he died of pneumonia at age 50 and the autopsy revealed marchiafava-bignami disease (mbd), striatal degeneration, pseudolaminar sclerosis of morel, atrophy of the corpus mamillare and pons, cortical cerebellar atrophy, pseudopellagra, and polyneuropathy. th ... | 1981 | 7211193 |
| mapping of sequence-tagged sites in rice by single strand conformation polymorphism. | the conditions for efficient single-strand conformation polymorphism (sscp) detection were examined for its application to mapping of dna regions in the rice genome. temperature for electrophoresis and glycerol concentrations in gel affected sscp patterns significantly. the optimal detection conditions for sscp also depends on the nucleotide sequences of fragments analyzed. fragments over 300 bp show complicated patterns depending on their nucleotide sequences and were not suitable for sscp anal ... | 1994 | 7719922 |
| insect resistant rice generated by introduction of a modified delta-endotoxin gene of bacillus thuringiensis. | as a first step towards development of insect resistant rice we have introduced a truncated delta-endotoxin gene, cryia(b) of bacillus thuringiensis (b.t.) which has specific biological activity against lepidopteran insects into a japonica rice. to highly express the cryia(b) gene in rice the coding sequence was extensively modified based on the codon usage of rice genes. transgenic plants efficiently expressed the modified cryia(b) gene at both mrna and protein levels. bioassays using r2 genera ... | 1993 | 7764096 |
| physical mapping of the 5s ribosomal rna genes on rice chromosome 11. | one 5s ribosomal rna gene (5s rdna) locus was localized on chromosome 11 of japonica rice by in situ hybridization. the biotinylated dna probe used was prepared by direct cloning and direct labeling methods, and the locus was localized to the proximal region of the short arm of chromosome 11 (11p1.1) by imaging methods. the distance between the signal site and the centromere is 4.0 arbitrary units, where the total length of the short arm is 43.3 units. the 5srdna locus physically identified and ... | 1994 | 7816019 |
| comparative analysis of microsatellite dna polymorphism in landraces and cultivars of rice. | genetic polymorphisms of ten microsatellite dna loci were examined among 238 accessions of landraces and cultivars that represent a significant portion of the distribution range for both indica and japonica groups of cultivated rice. in all, 93 alleles were identified with these ten markers. the number of alleles varied from a low of 3 or 4 at each of four loci, to an intermediate value of 9-14 at five loci, and to an extraordinarily high 25 at one locus. the numbers of alleles per locus are muc ... | 1994 | 7816026 |
| rflp mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. | moroberekan, a japonica rice cultivar with durable resistance to blast disease in asia, was crossed to the highly susceptible indica cultivar, co39, and 281 f7 recombinant inbred (ri) lines were produced by single seed descent. the population was evaluated for blast resistance in the greenhouse and the field, and was analyzed with 127 restriction fragment length polymorphism (rflp) markers. two dominant loci associated with qualitative resistance to five isolates of the fungus were tentatively n ... | 1994 | 7912216 |
| efficient transformation of rice (oryza sativa l.) mediated by agrobacterium and sequence analysis of the boundaries of the t-dna. | a large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with agrobacterium tumefaciens. the efficiency of transformation was similar to that obtained by the methods used routinely for transformation of dicotyledons with the bacterium. stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the r0, r1 and r2 generations. sequence analysis revealed that the ... | 1994 | 7920717 |
| alcohol consumption and insomnia in a sample of japanese alcoholics. | the amount of ethanol consumed by chronic alcoholics in a japanese slum area with persistent insomnia (n = 40) and those without it (n = 40) was compared using a questionnaire. for both groups, the present habitual consumption (phc) of ethanol per day was most frequently between 60 g and 150 g and no difference was observed between the two groups. in contrast, the maximum habitual consumption (mhc) of ethanol per day throughout the alcoholic history was found to be greater for the insomnia patie ... | 1994 | 8044125 |
| alcohol and coronary spasm. | alcohol is known to sometimes cause coronary spasm, the mechanism of which is still unknown. the authors monitored changes in plasma levels of prostanoids (thromboxane [tx b2], 6-keto prostaglandin f1 alpha [pgf1 alpha]), catecholamines (ca), serotonin (5-ht), cyclic nucleotides (cyclic adenosine monophosphate--camp, cyclic guanosine monophosphate--cgmp), and platelet aggregation after alcohol ingestion (japanese rice wine 400 ml) in 8 patients with alcohol-induced variant angina and 8 healthy m ... | 1994 | 8129199 |
| steroidal alkaloid toxicity to fish embryos. | embryos of two species of fish were evaluated for their suitability as model systems for steroidal alkaloid toxicity, the japanese rice fish, medaka (oryzius latipes) and the rainbow trout (oncorhynchus mykiss). additionally, the equine neurotoxic sesquiterpene lactone repin, was also tested. a probit program was used to evaluate the ec1, ec50 and ec99 as well as the associated confidence limits. the steroidal alkaloids tested were the solanum potato glycoalkaloids alpha-chaconine, alpha-solanin ... | 1993 | 8430437 |
| enigmatic double-stranded rna in japonica rice. | we have found a linear, 16 kb, double-stranded rna (dsrna) in symptomless japonica rice (oryza sativa l.) that is not found in indica rice (oryza sativa l.). the dsrna was detected in every tissue and at every developmental stage, and its copy number was approximately constant (about 20 copies/cell). double-stranded rna was also detected in two strains of oryza rufipogon (an ancestor of o. sativa). hybridization experiments indicated that the dsrna of o. rufipogon was homologous but not identica ... | 1993 | 8490131 |
| stringently and developmentally regulated levels of a cytoplasmic double-stranded rna and its high-efficiency transmission via egg and pollen in rice. | a very restricted amount of high-molecular-weight double-stranded rna (dsrna) has been found in healthy japonica rice plants. we discriminated dsrna-carrying rice plants from noncarriers. the endogenous dsrna was localized in the cytoplasm (about 100 copies per cell) and was transmissible to progeny plants by mating. in crosses between carriers and noncarriers, the rna was transmitted efficiently to f1 plants via both egg and pollen. the rice dsrna was maintained at an almost constant level by h ... | 1996 | 8806402 |
| the japanese rice genome research program. | 1996 | 8858341 | |
| characterization and genetic mapping of simple sequence repeats in the rice genome. | we searched partial sequences of over 22,706 rice cdna and 1220 genomic dna clones to find and characterize simple sequence repeats (ssrs) in the rice genome. the most frequently found repeated ssr motif in both cdna and genomic dna sequences was d(ccg/cgg)n. the second most frequently found ssr was d(ag/ct)n. in contrast with mammalian genomes, in which d(ac/gt)n sequences are the most abundant, d(ag/gt)n sequences were not frequently observed in rice. sequences containing d(ccg/cgg)n, d(ag/ct) ... | 1996 | 8946163 |
| novel dna probes capable of discriminating indica and japonica rice cultivars. | we obtained two dna clones, g93 (1.6kb) and g318 (1.7kb) from indica rice (oryza sativa l.) cultivar culture 340. southern hybridization analysis indicated that both dna probes hybridized with dna of indica cultivars but not with japonica cultivars. some javanica cultivars dnas did not hybridize with these probes. nucleotide sequence (400bp) of a part of g93 showed a 65.8% g + c content. these novel dna probes can be used for the study of evolutional fingerprinting of rice cultivars. | 1996 | 8988367 |
| structure, chromosomal location and expression of a rice gene encoding the microsome omega-3 fatty acid desaturase. | the omega-3 fatty acid desaturases are membrane-bound enzymes catalyzing the conversion of linoleic acid to linolenic acid in lipids, and are located both in the microsome and plastid envelopes as two different isoforms. a cdna encoding the microsome omega-3 fatty acid desaturase (osfad3) and the corresponding genomic clone were isolated from rice (oryza sativa l.). the osfad3 gene was composed of 8 exons and 7 introns. a microsatellite was present in the second exon of the osfad3 gene, showing ... | 1997 | 9049269 |
| transgenic rice (oryza sativa) endosperm expressing daffodil (narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin a biosynthesis. | rice (oryza sativa l.), the major food staple for more than two billion people, contains neither beta-carotene (provitamin a) nor c40 carotenoid precursors thereof in its endosperm. to improve the nutritional value of rice, genetic engineering was chosen as a means to introduce the ability to make beta-carotene into rice endosperm tissue. investigation of the biochemical properties of immature rice endosperm using [14c]-labelled substrates revealed the presence of geranyl geranyl diphosphate, th ... | 1997 | 9193076 |
| origin, dispersal, cultivation and variation of rice. | there are two cultivated and twenty-one wild species of genus oryza. o. sativa, the asian cultivated rice is grown all over the world. the african cultivated rice, o. glaberrima is grown on a small scale in west africa. the genus oryza probably originated about 130 million years ago in gondwanaland and different species got distributed into different continents with the breakup of gondwanaland. the cultivated species originated from a common ancestor with aa genome. perennial and annual ancestor ... | 1997 | 9291957 |
| rice genetic resources: history, conservation, investigative characterization and use in japan. | rice has been grown in japan for about 3000 years. although both japonica and indica varieties have been grown in japan, now japonica rices are grown. japanese rice breeding has used an ecological breeding approach. while emphasis in rice breeding in the 1940's and 1950's focussed on yield in recent decades quality has been of major importance. consumer preference and name recognition of high quality varieties, such as koshihikari, has resulted in slow acceptance of new varieties. rice germplasm ... | 1997 | 9291961 |
| synergistic enhancement of the postthaw growth of cryopreserved rice cells by oxygenated perfluorocarbon and pluronic f-68 | the beneficial effects were assessed of supplementing culture medium with oxygenated perfluorocarbon, both alone and in combination with 0.01 (w/v) pluronic f-68, on the postthaw viability, following cryopreservation, of suspension cultured cells of the japonica rice, oryza sativa cv. taipei 309. the mean viability, as assessed by triphenyl tetrazolium chloride reduction, of cells at 4 days after thawing was increased 20% over control by oxygenated perfluorodecalin (p < 0.05) in 100-ml glass jar ... | 1997 | 9367608 |
| agrobacterium-mediated transformation of élite indica and japonica rice cultivars. | a rapid, efficient, routine system has been established for agrobacterium tumefaciens-mediated production of hundreds of fertile transgenic plants from commercially important rice cultivars, including an indica cultivar, pusa basmati 1. calli induced from embryos of mature rice seeds were cocultivated with a. tumefaciens strain lba4404 carrying the plasmid ptok233, then exposed to hygromycin selection followed by an efficient regeneration system. based on the total number of calli co-cultivated, ... | 1997 | 9438257 |
| enhanced post-thaw viability of cryopreserved cells by oxygenated perfluorocarbon or pluronic f-68. | viability and growth following cryopreservation have been examined following (i) culture of oryza sativa cv. taipei 309 (japonica rice) in medium overlaying oxygenated perfluorocarbon (pfc) liquid (flutec pp6), and (ii) culture of two cvs. of japonica rice (taipei 309, tarom), and lolium multiflorum in media with pluronic f-68 (0.01-0.2% w/v). the mean viability of cv. taipei 309 rice cells following 4 days in medium overlaying oxygenated flutec (0.45 +/- 0.07; n = 20) was significantly (p < 0.0 ... | 1998 | 9507754 |
| transgenic rice plants harboring an introduced potato proteinase inhibitor ii gene are insect resistant. | we introduced the potato proteinase inhibitor ii (pinii) gene (pin2) into several japonica rice varieties, and regenerated a large number of transgenic rice plants. wound-inducible expression of the pin2 gene driven by its own promoter, together with the first intron of the rice actin 1 gene (act1), resulted in high-level accumulation of the pinii protein in the transgenic plants. the introduced pin2 gene was stably inherited in the second, third, and fourth generations, as shown by molecular an ... | 1996 | 9630927 |
| examination of number-average degree of polymerization and molar-based distribution of amylose by fluorescent labeling with 2-aminopyridine. | suitable conditions for the fluorescent labeling of the reducing residue of amylose with 2-aminopyridine were examined. amylose of up to 38.5 nmol was labeled with a constant labeling efficiency. the same efficiencies were obtained for amyloses having a number-average degree of polymerization (dpn) of 521-4400. the analysis of labeled amylose on size-exclusion hplc with refractive index and fluorescence detection enabled the determination of dpn and dp distribution on a molar basis. the analysis ... | 0 | 9648249 |
| nucleotide sequence, genetic organization and expression strategy of the double-stranded rna associated with the '447' cytoplasmic male sterility trait in vicia faba. | the entire nucleotide sequence of the double-stranded (ds) rna associated with the unconventional '447' cytoplasmic male sterility (cms) trait in vicia faba was determined from overlapping cdna clones and by rt-pcr. confirming previous observations, it was found that the negative-strand was continuous and 17,635 nt long, while the positive-strand featured an interruption, probably a nick, that could potentially define two subgenomic rnas of 2735 nt and 14,900 nt, with the smaller rna being locat ... | 1998 | 9780039 |
| [genetic analysis on a new sterile locus discovered in hybrids between indica and japonica rice (oryza sativa l.)]. | japonica line 02428, possessing wide compatibility gene s-5n, showed semi-sterility as crossed with indica variety ir24. genetic analysis was conducted on the spikelet fertility in segregation generations from the crosses of 02428 with ir24, pecos and akihikara. the results indicated that the sterility in hybrids between 02428 and ir24 was controlled by a single non-allelic to s-5 gene and its inheritance fitted to one-locus sporo-gametophytic interaction model. pecos, an american variety, has a ... | 1998 | 9800438 |
| molecular characterization of two endogenous double-stranded rnas in rice and their inheritance by interspecific hybrids. | we completely sequenced 13,936 nucleotides (nt) of a double-stranded rna (dsrna) of wild rice (w-dsrna). a single long open reading frame (13,719 nt) containing the conserved motifs of rna-dependent rna polymerase and rna helicase was located in the coding strand. the identity between entire nucleotide sequence of w-dsrna and that of the dsrna of temperate japonica rice (j-dsrna, 13,952 nt) was 75.5%. a site-specific discontinuity (nick) was identified at nt 1,197 from the 5' end of the coding s ... | 1999 | 10066741 |
| purification, characterization, and application of an acid urease from arthrobacter mobilis. | it has been shown that urea in fermented beverages and foods can serve as a precursor of ethylcarbamate, a potential carcinogen, and acid urease is an effective agent for removing urea in such products. we describe herein the purification and characterization of a novel acid urease from arthrobacter mobilis sam 0752 and show its unique application for the removal of urea from fermented beverages using the japanese rice wine, sake, as an example. the purified acid urease showed an optimum ph for ... | 1999 | 10194859 |
| purification, characterization, and cdna structure of isoamylase from developing endosperm of rice. | isoamylase (ec 3.2.1.68) in rice (oryza sativa l.) was efficiently purified within a day to homogeneity, as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), from developing endosperm by sequential use of q sepharose hp anion-exchange chromatography, ammonium sulfate fractionation, and tskgel g4000swxl and g3000swxl gel filtration chromatography. although the protein exhibited a molecular size of ca. 83 kda on sds-page, the apparent size of the native enzyme was ... | 1999 | 10333591 |
| unusual inheritance of evolutionarily-related double-stranded rnas in interspecific hybrid between rice plants oryza sativa and oryza rufipogon. | endogenous, 14 kb double-stranded rnas (dsrnas) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, oryza sativa l.) and in wild rice (o. rufipogon, an ancestor of o. sativa). a comparison of the nucleotide and deduced amino acid sequences of the core regions of the rna-dependent rna polymerase domains found in these three dsrnas suggested that these dsrnas probably evolved independently within each host plant from a common ancestor. these ds ... | 1999 | 10380800 |
| identification of heading date quantitative trait locus hd6 and characterization of its epistatic interactions with hd2 in rice using advanced backcross progeny. | a backcrossed population (bc(4)f(2)) derived from a cross between a japonica rice variety, nipponbare, as the recurrent parent and an indica rice variety, kasalath, as the donor parent showed a long-range variation in days to heading. quantitative trait loci (qtl) analysis revealed that two qtl, one on chromosome 3, designated hd6, and another on chromosome 2, designated hd7, were involved in this variation; and hd6 was precisely mapped as a single mendelian factor by using progeny testing (bc(4 ... | 2000 | 10655238 |
| genomic organization of the 260 kb surrounding the waxy locus in a japonica rice | the present study was carried out to characterize the molecular organization in the vicinity of the waxy locus in rice. to determine the structural organization of the region surrounding waxy, contiguous clones covering a total of 260 kb were constructed using a bacterial artificial chromosome (bac) library from the shimokita variety of japonica rice. this map also contains 200 overlapping subclones, which allowed construction of a fine physical map with a total of 64 hindiii sites. during the c ... | 1999 | 10659779 |
| international rice genome sequencing project: the effort to completely sequence the rice genome. | the international rice genome sequencing project (irgsp) involves researchers from ten countries who are working to completely and accurately sequence the rice genome within a short period. sequencing uses a map-based clone-by-clone shotgun strategy; shared bacterial artificial chromosome/p1-derived artificial chromosome libraries have been constructed from oryza sativa ssp. japonica variety 'nipponbare'. end-sequencing, fingerprinting and marker-aided pcr screening are being used to make sequen ... | 2000 | 10712951 |
| effect of spikelet position on rice anther culture efficiency. | the potential of anthers from different parts of the panicle to induce callus was investigated with the japonica rice variety taipei 309. the results showed that the callusing abilities of anthers from different spikelet positions were significantly different. after plating 4483, 4496, 4348 anthers from the basal, middle and top parts, the percentage of anthers forming calli was 20% in the basal part, 12% in the middle part and 8% in the top part. the anthers of basal parts containing pollen at ... | 2000 | 10717321 |
| [biological actions of acetaldehyde]. | acetaldehyde (ach), the first metabolite of ethanol (etoh), is a chemically reactive and pharmacologically active compound. the author has been engaged in the study of ach in cooperation with many researchers for three decades. we have found many biological actions of ach which cause cardiovascular symptoms after drinking and also inhibited etoh absorption via the canine and rat intestinal tract. this report covers the following five points. 1. the subjects were classified into a non-flushing gr ... | 1999 | 10723960 |
| identification of alpha-d-glucosylglycerol in sake. | alpha-d-glucosylglycerol (gg) was found for the first time in sake (japanese rice wine) in an amount of about 0.5%. gg was also found in miso and mirin which had been brewed by using koji. gg was hydrolyzed into glucose and glycerol in an equimolar ratio with maltase (ec 3.2.1.20, alpha-glucosidase from yeast), but not with emulsin (ec 3.2.1.21, beta-glucosidase from almond). the retention times and mass spectra of trimethylsilyl derivatives by a gc-ms analysis of gg in sake were comparable to t ... | 2000 | 10737196 |
| identification and chromosomal localization of a transcriptionally active retrotransposon of ty3-gypsy type in rice. | a dna fragment representing a transcriptionally active retrotransposon of ty3-gypsy type was isolated and characterized from rice (oryza sativa l.). the fragment (named rire9) includes the coding sequences for the c-terminal part of the rnase h domain and the n-terminal part of the integrase domain in the polyprotein region. northern blot hybridization indicated that this element was expressed in rice leaves and stems, suggesting that it is potentially active to transpose under normal growth con ... | 2000 | 10791831 |
| identification of a signal peptide for oryzacystatin-i. | a previously unidentified extension of an open reading frame from the genomic dna of japonica rice (oryza sativa l.) encoding oryzacystatin-i (oc-i; access. m29259, protein id aaa33912.1) has been identified as a 5' gene segment coding for the oc-i signal peptide. the signal peptide appears to direct a pre-protein (spoc-i; accession no. af164378) to the endoplasmic reticulum, where it is processed into the mature form of oc-i. the start codon of spoc-i begins 114 bp upstream from that previously ... | 2000 | 10805458 |
| quantification of total genomic dna and selected repetitive sequences reveals concurrent changes in different dna families in indica and japonica rice. | this paper describes a fluorescence in situ hybridization (fish) analysis of three different repetitive sequence families, which were mapped to mitotic metaphase chromosomes and extended dna fibers (edfs) of the two subspecies of rice (oryza sativa), indica and japonica (2n = 2x = 24). the repeat families studied were (1) the tandem repeat sequence a (trsa), a functionally non-significant repeat; (2) the [ttta-ggg]n telomere sequence, a non-transcribed, tandemly repeated but functionally signifi ... | 2000 | 10821172 |
| [stable inheritance of hpt gene in transgenic rice plants mediated by biolistic bombardment]. | japonica rice cv 77170 was transformed with pbi222 carrying hygromycin phosphotransferase gene (hpt) by using biolistic bombardment and fertile transgenic rice plants were obtained. in t1 and most t2 generation the performance of hygromycin resistance was normal. only in t2 progeny of sg-15 some lines showed decreased resistance. mendelian inheritance of hygromycin resistance was showed as single dominant locus and proved by southern blotting analysis in t1 and t2 generation of all transgenic pl ... | 2000 | 10883536 |
| thermal and physicochemical properties of rice grain, flour and starch. | three types of rices, namely, thailand rice (indica), nipponbare (japonica), and himenomochi (japonica waxy), in grain, flour, and starch forms have been studied for their thermal and physicochemical properties. in grain form, indica was slender and japonica rices were bold and thick. indica had the highest protein and amylose equivalent. protein contents in isolated starches varied from 0.2 to 0.9%. cooked indica grain was hardest and waxy rice was softest; stickiness was highest in japonica ri ... | 2000 | 10898601 |
| dull: rice mutants with tissue-specific effects on the splicing of the waxy pre-mrna. | in the endosperm of japonica rice, du-1 and du-2 mutations cause the reduction of amylose contents. it was previously shown that the wx(b) allele of rice, which is predominantly distributed in japonica rice, has a mutation in the 5' splice site of intron 1 resulting in the creation of two weak 5' splice sites within exon 1. in du-1 and du-2 mutants, spliced wx(b) transcripts were highly reduced, whereas the processing of transcripts derived from three other genes highly expressed in endosperm wa ... | 2000 | 10972871 |
| [isolation and cytological identification of a desynaptic variant in rice]. | a sterile variant was found from the population of a japonica rice variety lunhui 422. cytological investigation was conducted in meiosis. it is showed that the sterility was caused by desynapsis. as there were 8.69 bivalents in average occurred to be desynaptic, moreover, the most number of the desynaptic bivalents reached 12 at post diakinsis. it can be considered that the desynaptic variation is a complete desynapsis found in rice so far. | 2000 | 10979187 |
| photosynthesis and translocation of assimilates in rice plants following phloem feeding by the planthopper nilaparvata lugens (homoptera: delphacidae). | experiments were conducted to measure the effect of feeding by the planthopper nilaparvata lugens (stål) on photosynthesis and the translocation of assimilates in rice plants, oryza sativa l. we used mature japonica rice plants and applied the 13co2 feeding method to evaluate those physiological effects. the photosynthetic rate was suppressed by n. lugens infestation, especially at the lower leaf position, with rates 30% lower than that of control plants at the booting stage. leaf nitrogen conce ... | 2000 | 10985030 |
| uv radiation-sensitive norin 1 rice contains defective cyclobutane pyrimidine dimer photolyase. | norin 1, a progenitor of many economically important japanese rice strains, is highly sensitive to the damaging effects of uvb radiation (wavelengths 290 to 320 nm). norin 1 seedlings are deficient in photorepair of cyclobutane pyrimidine dimers. however, the molecular origin of this deficiency was not known and, because rice photolyase genes have not been cloned and sequenced, could not be determined by examining photolyase structural genes or upstream regulatory elements for mutations. we ther ... | 2000 | 11006332 |
| ribozyme-mediated resistance to rice dwarf virus and the transgene silencing in the progeny of transgenic rice plants. | a hammerhead ribozyme (rz) with long hybridizing arms targeting the mrna of rice dwarf virus (rdv) segment 5 and a mutated nonfunctional ribozyme (mrz) were constructed. as predicted, rz transcribed in vitro cleaved the target mrna of rdv segment 5 into two fragments of 138 and 238 nucleotides in length. the rz and mrz genes were each placed under the control of the camv 35s promoter and used to transform japonica rice variety 'tongling no. 1' via agrobacterium tumefaciens. a total of 32 indepen ... | 2000 | 11032368 |
| characterization of acetic acid bacteria in traditional acetic acid fermentation of rice vinegar (komesu) and unpolished rice vinegar (kurosu) produced in japan. | bacterial strains were isolated from samples of japanese rice vinegar (komesu) and unpolished rice vinegar (kurosu) fermented by the traditional static method. fermentations have never been inoculated with a pure culture since they were started in 1907. a total of 178 isolates were divided into groups a and b on the basis of enterobacterial repetitive intergenic consensus-pcr and random amplified polymorphic dna fingerprinting analyses. the 16s ribosomal dna sequences of strains belonging to eac ... | 2001 | 11157275 |
| nonsense-mediated decay of mutant waxy mrna in rice. | two rice (oryza sativa) waxy mutations of the japonica background were shown to contain approximately 20% of the fully spliced mrna relative to the wild type. sequencing analysis of the entire waxy genes of the two mutants revealed the presence of premature translation termination codons in exon 2 and exon 7. these results indicated that the lower accumulation of fully spliced rna in the mutants was caused by nonsense-mediated decay (nmd), which is an rna surveillance system universally found in ... | 2001 | 11244118 |
| identification of yac clones containing the mutable slender glume locus slg in rice (oryza sativa l.). | a mutable slender glume gene slg, which often reverts to the wild-type state, was induced by gamma-ray irradiation of seeds of the japonica rice cultivar 'gimbozu'. the final goal was to understand whether the slender glume mutation was associated with the insertion of a transposable element, utilizing map-based cloning techniques. the rflp (restriction fragment length polymorphism) analysis revealed that the slg locus was located between two rflp loci, xnpb33 and r1440, on chromosome 7 with rec ... | 2001 | 11269342 |
| [improvement of transformation frequency of rice mediated by agrobacterium]. | the factors influencing the frequency of rice transformation mediated by agrobacterium have been investigated by using 16 commercially important indica and japonica rice cultivars or lines. the main results were as following: for most rice cc medium was the best for both callus initiation and subculture. with supplement of 2.5-5 mg/l aba the quality of calli can be improved. the concentration of selective agent for indica rice callus was lower than that for japonica rice callus. agrobacterium tu ... | 2001 | 11329877 |
| identification of defense-related rice genes by suppression subtractive hybridization and differential screening. | identification of host genes involved in defense responses is one of most critical steps leading to the elucidation of disease resistance mechanisms in plants. in this study, two different cloning strategies were employed to identify defense-related genes from a tropical japonica rice cultivar (oryza sativa cv. drew). with the use of bacterial colony arrays, differential screening of a blast fungus (pyricularia grisea)-induced rice cdna library led to the isolation of 22 distinct rice genes that ... | 2001 | 11332734 |
| mapping of qtls associated with cytosolic glutamine synthetase and nadh-glutamate synthase in rice (oryza sativa l.). | ninety-eight backcross inbred lines (bc1f6) developed between nipponbare, a japonica rice, and kasalath, an indica rice were employed to detect putative quantitative trait loci (qtls) associated with the contents of cytosolic glutamine synthetase (gs1; ec 6.3.1.2) and nadh-glutamate synthase (nadh-gogat; ec 1.4.1.14) in leaves. immunoblotting analyses showed transgressive segregations toward lower or greater contents of these enzyme proteins in these backcross inbred lines. seven chromosomal qtl ... | 2001 | 11432939 |
| grain sink strength may be related to the poor grain filling of indica-japonica rice (oryza sativa) hybrids. | the physiological and biochemical factors contributing to poor grain filling of indica-japonica rice (oryza sativa l.) hybrids were studied by analyzing the role of grain sink strength in dry matter accumulation of grains of two types of rice cultivars, yayou 2 (an indica-japonica hybrid) and yanjing 2 (a japonica cultivar). carbon dioxide enrichment and plant hormone application were imposed at anthesis and the number of endosperm cells, dry matter accumulation and the activities of some sugar- ... | 2001 | 11473706 |
| two types of hkt transporters with different properties of na+ and k+ transport in oryza sativa. | it is thought that na+ and k+ homeostasis is crucial for salt-tolerance in plants. to better understand the na+ and k+ homeostasis in important crop rice (oryza sativa l.), a cdna homologous to the wheat hkt1 encoding k+-na+ symporter was isolated from japonica rice, cv nipponbare (ni-oshkt1). we also isolated two cdnas homologous to ni-oshkt1 from salt-tolerant indica rice, cv pokkali (po-oshkt1, po-oshkt2). the predicted amino acid sequence of ni-oshkt1 shares 100% identity with po-oshkt1 and ... | 2001 | 11489190 |
| establishment of a new cross of the rice blast fungus derived from japanese differential strain ina168 and hermaphroditic rice pathogen guy11. | mating experiments between magnaporthe grisea japanese rice pathogens and guy11, a hermaphroditic fertile rice pathogen, were done aimed at identification of avirulence genes. a cross named cross 2107 with thirty-six random progenies was obtained. segregation analyses of genetic markers found that the cross was less suitable for genetic analysis. backcrosses with cross 2107 progenies and guy11 were done and another cross named cross 5307 with sixty-five progenies was obtained. a locus controllin ... | 2001 | 11515527 |
| visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor fish to chromosomes and extended dna fibers. | high-resolution fluorescence in situ hybridization (fish) on interphase and pachytene nuclei, and extended dna fibers enabled microscopic distinction of dna sequences less than a few thousands of base pairs apart. we applied this technique to reveal the molecular organization of telomere ends in japonica rice (oryza sativa ssp. japonica), which consist of the arabidopsis type tttaggg heptameric repeats and the rice specific subtelomeric tandem repeat sequence a (trsa). southern hybridizations of ... | 2001 | 11587512 |
| [the factors of improving rice transformation efficiency]. | the factors influencing the rice transformation frequency have been investigated by using 8 indica and japonica rice cultivars during the transformation procedures, such as induction of calli, selection, and regeneration. the following is the results: the immature embryos were pretreated in 4 degrees c for 4 or 7 days before inoculation, and the regeneration frequency of calli would be increased. high osmotic treatment may increase the possibility of transformation, especially for calli of 6 mon ... | 2001 | 11695265 |
| toward a cytological characterization of the rice genome. | rice (oryza sativa l.) will be the first major crop, as well as the first monocot plant species, to be completely sequenced. integration of dna sequence-based maps with cytological maps will be essential to fully characterize the rice genome. we have isolated a set of 24 chromosomal arm-specific bacterial artificial chromosomes to facilitate rice chromosome identification. a standardized rice karyotype was constructed using meiotic pachytene chromosomes of o. sativa spp. japonica rice var. nippo ... | 2001 | 11731505 |
| behaviors of d- and l-lactic acids during the brewing process of sake (japanese rice wine). | the amounts of d- and l-lactic acids during the brewing process of sake were determined by capillary electrophoresis using 2-hydroxypropyl-beta-cyclodextrin as a chiral selector. because l-lactic acid, which prevents the growth of nonuseful microorganisms, is a raw material of sake, the ratio of l-lactic acid to total lactic acid is almost 1.0 at the initial stage of sake brewing. during brewing, the ratio decreased gradually and finally reached 0.39. yeast (saccharomyces cerevisiae) for sake br ... | 2002 | 11829643 |
| relationship between ultraviolet-b sensitivity and cyclobutane pyrimidine dimer photorepair in rice. | among indica rice cultivars (oryza sativa l. cvs.) that belong to the aus ecotype from the tropical bengal region, where the amount of ultraviolet-b (uv-b) radiation in the solar radiation is relatively great, marich-bati cultivar has exhibited resistance to uv-b radiation, while surjamkhi cultivar appeared to be less resistant. we have examined the susceptibility to cyclobutane pyrimidine dimer (cpd) induction by uv-b radiation and the ability to photorepair cpds using these two cultivars. uv-b ... | 2001 | 11840646 |
| genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice. | immunocytological studies in this laboratory have suggested that nadh-dependent glutamate synthase (nadh-gogat; ec 1.4.1.14) in developing organs of rice (oryza sativa l. cv. sasanishiki) is involved in the utilization of glutamine remobilized from senescing organs through the phloem. because most of the indica cultivars contained less nadh-gogat in their sink organs than japonica cultivars, over-expression of nadh-gogat gene from japonica rice was investigated using kasalath, an indica cultivar ... | 2002 | 11912234 |
| a fine physical map of the rice chromosome 4. | as part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome (bac)-based physical map of the oryza sativa japonica nipponbare chromosome 4 through an integration of 114 sequenced bac clones from a taxonomically related subspecies o. sativa indica guangluai 4 and 182 rflp and 407 expressed sequence tag (est) markers with the fingerprinted data of the nipponbare genome. the map consists of 11 contigs with a total length of 34.5 ... | 2002 | 11997348 |
| identification of rice black streaked dwarf virus in different cereal crops with dwarfing symptoms in china. | this report describes isolation of virus particles from plants of rice, maize, wheat and sorghum with symptoms of dwarfing collected from two provinces of china, purification of double-stranded rna (dsrna) from the virus particles, and synthesis of full-length cdnas of genome segments 9 (s9) and 10 (s10) by reverse transcription-polymerase chain reaction (rt-pcr). sequence analysis showed that the s9 sequences of the chinese isolates and a japanese rice black-streaked disease virus (rbsdv) isola ... | 2001 | 12083334 |
| rice (oryza sativa l.) cultivars tolerant to high temperature at flowering: anther characteristics. | we examined the relationship between morphological characteristics of anthers and fertility in japonica rice cultivars subjected to high temperature (37.5(26 degrees c day/night) at flowering. percentage fertility was negatively correlated with the number of cell layers that separated the anther locule from the lacuna that formed between the septum and the stomium. the cell layers consisted of the remaining septum and degraded tapetum, and serve to keep the adjacent two locules closed. anther de ... | 2002 | 12102523 |
| qualitative analysis of some carboxylic acids by ion-exclusion chromatography with atmospheric pressure chemical ionization mass spectrometric detection. | a simple, selective and sensitive method for the determination of carboxylic acids has been developed. a mixture of formic, acetic, propionic, valeric, isovaleric, isobutyric, and isocaproic acids has been separated on a polymethacrylate-based weak acidic cation-exchange resin (tsk gel oa pak-a) based on an ion-exclusion chromatographic mechanism with detection using uv-photodiode array, conductivity and atmospheric pressure chemical ionization mass spectrometry (apci-ms). a mobile phase consist ... | 2002 | 12108651 |
| [[selectivity of staple food-taking pattern by cohort analysis]]. | changing dietary patterns in japan are reviewed in relation to characteristics such as age, sex, educational status, occupation, region, and migration. data are from a field survey undertaken in miyagi and kumamoto prefectures in 1981. the focus is on the change from a traditional japanese rice-based diet to a western style of nutrition. (summary in eng) | 1986 | 12314555 |
| [development and prospect of plant mutation breeding induced by aviation and spaceflight in china]. | since 1987, about fifty species, three hundred varieties of crops have been sent in recoverable satellites or high altitude balloon (hab). the selected lines include a high-yield, disease-resist, and hybrid-vigorous rice line, a recovered line of japonica rice restoring sterility of indica rice, a new green pepper strain which grows fruits over 500 g, a disease-resist, high-quality tomato line, a big-pod rape line, a big-fruit white lotus line and a big-bulb lily. the special conditions in the o ... | 1999 | 12434817 |
| search for and analysis of single nucleotide polymorphisms (snps) in rice (oryza sativa, oryza rufipogon) and establishment of snp markers. | we searched for snps in 417 regions distributed throughout the genome of three oryza sativa ssp. japonica cultivars, two indica cultivars, and a wild rice (o. rufipogon). we found 2800 snps in approximately 250,000 aligned bases for an average of one snp every 89 bp, or one snp every 232 bp between two randomly selected strains. graphic representation of the frequency of snps along each chromosome showed uneven distribution of polymorphism-rich and -poor regions, but little obvious association w ... | 2002 | 12465716 |
| isolation, characterization and expression of the human factor in the germline alpha (figla) gene in ovarian follicles and oocytes. | the factor in the germline alpha (figalpha) transcription factor regulates expression of the zona pellucida proteins zp1, zp2 and zp3 and is essential for folliculogenesis in the mouse. using the published mouse figla sequence, blast searches identified a human chromosome 2 bac clone with high sequence identity. using pcr primers derived from this clone, amplicons derived from ovarian follicles and mature oocytes revealed 100% identity with the appropriate human bac clone, the expected homology ... | 2002 | 12468641 |
| [genetic analysis of blast resistance in japonica rice landrace heikezijing from taihu region]. | japonica rice landrace heikezijing (hkzj) from taihu region is highly resistant to several chinese and japanese differential strains of magnaporthe grisea. the f1, f2 and ril populations from the cross between the resistant variety heikezijing and the susceptible variety lijiangxintuanheigu (ljxthg) were inoculated by spray with two strains of ken 54-04 and hoku 1 in seedling stages. based on the r:s ratios of segregation in f1, f2 and ril populations it was showed that there were two independen ... | 2002 | 12561228 |
| mapping of a gene responsible for the difference in amylopectin structure between japonica-type and indica-type rice varieties. | the present investigation revealed that the alk and gel(t) genes, which cause the differences between a japonica rice variety nipponbare and an indica rice variety kasalath in terms of the disintegration of endosperm starch granules in alkali solution and their gelatinisation in a 4 m urea solution, respectively, cosegregated in backcross inbred lines derived from a cross between the two varieties. the segregation pattern of the profile for amylopectin chain-length, which was distinguished by en ... | 2002 | 12579422 |
| origin of cytoplasm substituted rice cultivars found in japan. | genetic variation of japanese rice cultivars were examined. five of 450 lowland cultivars and another five of 200 upland cultivars were determined as the indica type by using isozyme genotypes and the remainder were of the japonica type. the major characteristics of these indica cultivars, revealed a slender shape of grains, a short apiculus hair length, a positive allele for ph reaction, and allele-3 for the pgd1 locus. three of these indica cultivars showed a non-deletion orf100, which is esse ... | 2002 | 12582511 |
| cloning of cdna for udp-glucose pyrophosphorylase and the expression of mrna in rice endosperm. | rice endosperm udp-glucose pyrophosphorylase (ugpase) cdna clones were isolated by screening a lambda zap ii library prepared from poly (a(+)) rna of japonica rice (cv sasanishiki) endosperm with a probe of potato ugpase cdna. one cdna clone, possessing about 1,700 nucleotides, contained the complete open reading frame of rice ugpase. at the nucleotide-sequence level, the ugpase cdna of rice endosperm had high homology with the ugpase cdna of barley endosperm (84%) and potato tuber (71%). the ca ... | 2002 | 12582522 |
| different maternal origins of japanese lowland and upland rice populations. | plastid subtype id (ps-id) sequences were determined from sequence data based on ca repeats between genes rpl16 and rpl14 in japanese lowland and upland cultivars. the ps-id sequences of japanese rice cultivars showed that there are different maternal origins between lowland and upland cultivars. one subtype, 6c7a, of ps-id sequences was predominant in all but one japanese lowland cultivar and carried a combination of the indica-specific subtype 8c8a and japonica-specific nuclear markers for the ... | 2002 | 12582602 |
| isolation, characterization, and mapping of the stay green mutant in rice. | leaf color turns yellow during senescence due to the degradation of chlorophylls and photosynthetic proteins. a stay green mutant was isolated from the glutinous japonica rice hwacheong- wx through n-methyl-n-nitrosourea mutagenesis. leaves of the mutant remained green, while turning yellow in those of the wild-type rice during senescence. the stay green phenotype was controlled by a single recessive nuclear gene, tentatively symbolized as sgr(t). all the phenotypic characteristics of the mutant ... | 2002 | 12582654 |
| inheritance and expression of the cry1ab gene in bt ( bacillus thuringiensis) transgenic rice. | the inheritance and expression patterns of the cry1ab gene were studied in the progenies derived from different bt ( bacillus thuringiensis) transgenic japonica rice lines under field conditions. both mendelian and distorted segregation ratios were observed in some selfed and crossed f(2) populations. crosses between japonica intra-subspecies had no significant effect on the segregation ratios of the cry1ab gene, but crossing between japonica and indicainter-subspecies led to distorted segregati ... | 2002 | 12582680 |
| genetic differentiation of wild relatives of rice as assessed by rflp analysis. | to study genetic diversity and relationships of wild relatives of rice, 58 accessions of oryza rufipogon, oryza nivara, oryza sativa f. spontanea and the cultivated oryza sativa, representing a wide range of their distribution, were analyzed using the restriction fragment length polymorphism (rflp) technique. all 30-used rflp probes detected polymorphisms among the oryza accessions, with an average of 3.8 polymorphic fragments per probe. considerable genetic diversity was scored among the oryza ... | 2002 | 12582876 |
| one large-insert plant-transformation-competent bibac library and three bac libraries of japonica rice for genome research in rice and other grasses. | we report one large-insert bibac library and three bac libraries for japonica rice cv nipponbare. the bibac library was constructed in the hindiii site of a plant-transformation-competent binary vector (pcld04541) and the three bac libraries were constructed in the bamhi, hindiii and ecori sites of a bac vector (pecbac1), respectively. each library contains 23,040 clones, has an average insert size of 130 kb, 170 kb, 150 kb and 156 kb, and covers 6.7x, 8.7x, 7.7x and 8.0 x rice haploid genomes, ... | 2002 | 12582934 |
| transgenic expression of trypsin inhibitor cme from barley in indica and japonica rice, confers resistance to the rice weevil sitophilus oryzae. | indica and japonica rice (oryza sativa l.) plants were transformed by particle bombardment with the itr1 gene encoding the barley trypsin inhibitor bti-cme, under the control of its own promoter that confers endosperm specificity, and the maize ubiquitin promoter. from 38 independent transgenic lines of indica (breeding line ir58) and 15 of the japonica (cv senia) selected, 22 and 11, respectively, expressed the barley inhibitor at detectable levels. the transgene was correctly translated as ind ... | 2003 | 12650522 |
| stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. | amylose content in rice endosperm is a key determinant of eating and cooking quality. in the present study, a chimeric antisense construct, which contained a 756-bp antisense waxy (wx) gene dna fragment from rice and the gusa coding sequence, both fused to the 3.1-kb rice wx promoter, was efficiently introduced into several elite rice cultivars, both of japonica and indica type, via agrobacterium. more than 200 independent transgenic lines were produced and integration transgene was confirmed by ... | 2003 | 12650526 |
| molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types. | hsp100 protein is an important component of the heat-shock response in diverse organisms. using specific primers based on cdna sequence, rice hsp101 gene was pcr-amplified and sequenced. southern analysis revealed that there appears to be a single gene per haploid genome coding for hsp101 protein in rice. northern analysis showed that expression of hsp101 transcript is strictly heat-inducible and induction is transient in nature. in the temperature regime tested, 45 degrees c treatment to intact ... | 2003 | 12650620 |
| methane emission from fields with differences in nitrogen fertilizers and rice varieties in taiwan paddy soils. | flooded rice fields are one of the major biogenic methane sources. in this study, methane emission rates were measured after transplanting in paddy fields with application of two kinds of nitrogen fertilizers (ammonium sulfate, nh4+-n and potassium nitrate, no3(-)-n) and with two kinds of rice varieties (japonica and indica). the experiment was conducted in fields located at tainan district agricultural improvement station in chia-yi county (23 degrees 25'08"n, 120 degrees 16'26"e) of southern t ... | 2003 | 12653295 |
| highly efficient production and characterization of t-dna plants for rice ( oryza sativa l.) functional genomics. | we investigated the potential of an improved agrobacterium tumefaciens-mediated transformation procedure of japonica rice ( oryza sativa l.) for generating large numbers of t-dna plants that are required for functional analysis of this model genome. using a t-dna construct bearing the hygromycin resistance ( hpt), green fluorescent protein ( gfp) and beta-glucuronidase ( gusa) genes, each individually driven by a camv 35s promoter, we established a highly efficient seed-embryo callus transformat ... | 2003 | 12677401 |
| salinity tolerance of japonica and indica rice (oryza sativa l.) at the seedling stage. | in order to identify the degree of salinity tolerance of the indica and japonica rice groups, 10 varieties were tested under saline and non-saline conditions. twelve-day-old seedlings were grown in normal culture solution, then initially salinized at an electrical conductivity (ec) of 6 ds/m for 4 days, and finally salinized at an ec of 12 ds/m for the next 14 days. the growth parameters, and na and k absorption in the shoot were measured to characterize the tolerance level of the two rice group ... | 2003 | 12687373 |
| mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between oryza rufipogon and the oryza sativa cultivar jefferson. | an advanced backcross population between an accession of oryza rufipogon (irgc 105491) and the u.s. cultivar jefferson (oryza sativa ssp. japonica) was developed to identify quantitative trait loci (qtls) for yield, yield components and morphological traits. the genetic linkage map generated for this population consisted of 153 ssr and rflp markers with an average interval size of 10.3 cm. thirteen traits were examined, nine of which were measured in multiple environments. seventy-six qtls above ... | 2003 | 12736777 |
| introduction of wx transgene into rice wx mutants leads to both high- and low-amylose rice. | the waxy (wx) gene encodes a granule-bound starch synthase (gbss) that plays a key role in the amylose synthesis of rice and other plant species. two functional wx alleles of rice exist: wx(a), which produces a large amount of amylose, and wx(b), which produces a smaller amount of amylose because of the mutation at the 5' splice site of intron 1. wx(b) is largely distributed in japonica cultivars, and high amylose cultivars do not exist in japonica cultivars. we introduced the cloned wx(a) cdna ... | 2003 | 12773633 |
| production of doubled haploids through anther culture of m1 rice plants derived from mutagenized fertilized egg cells. | to produce stable mutants from mankeumbyeo, a japonica rice (oryza sativa l.) variety, we estimated the mutation efficiency of ethyl methane sulfonate (ems) and n-methyl-n-nitrosourea (mnu) on fertilized egg cells using doubled haploids (dhs) derived from anther culture of m1 plants. m1 seed production and germination were higher in 1 mm mnu than in 94.2 mm ems. a total of 68 dhs (35.4%) were regenerated by anther culture of m1 plants. twenty-one dhs (30.9%) were stable mutants, 14 dhs (20.6%) w ... | 2003 | 12845474 |
| collection, mapping, and annotation of over 28,000 cdna clones from japonica rice. | we collected and completely sequenced 28,469 full-length complementary dna clones from oryza sativa l. ssp. japonica cv. nipponbare. through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). mapping of the cdna clones to genomic dna revealed that there are 19,000 to 20,500 transcription units in the rice genome. protein informatics analysis against the interpro database revealed the existence of proteins presented in rice bu ... | 2003 | 12869764 |
| inheritance of oryza sativa endornavirus in f1 and f2 hybrids between japonica and indica rice. | we have found a 14 kbp double-stranded rna (dsrna) in many cultivars of japonica rice (oryza sativa l.) but not in any cultivars of indica rice. this dsrna is an rna replicon with plasmid-like properties and is proposed to be a novel dsrna virus, oryza sativa endornavirus (osv). reciprocal crosses between the osv-carrier japonica variety (nipponbare) and the osv-free indica variety (ir 26 or kasalath) were performed to investigate whether osv can be transmitted to f1 hybrids. when ir 26 and nipp ... | 2003 | 12893964 |
| mapping of quantitative trait loci associated with ultraviolet-b resistance in rice (oryza sativa l.). | the detection of quantitative trait loci (qtls) associated with uv-b resistance in rice should allow their practical application in breeding for such a complex trait, and may lead to the identification of gene characteristics and functions. considerable variation in uv-b resistance exists within cultivated rice (oryza sativa l.), but its detailed genetic control mechanism has not been well elucidated. we detected putative qtls associated with the resistance to enhanced uv-b radiation in rice, us ... | 2003 | 12898021 |
| [changes in the cadmium content of rice during the milling process]. | milled rice and bran were prepared from brown rice (oryza sativa japonica) using a commercial scale rice-mill to investigate the changes in cadmium (cd) content caused by the milling process. rice sample solution was prepared by microwave digestion using hno3 and hf, then analyzed by icp-ms. cd was measured at m/z 114 in interference correction with moo and sn. the digestion method was validated with 4 kinds of certified reference material of "rice flour". we found that the cd content was reduce ... | 2003 | 12968468 |
| golden indica and japonica rice lines amenable to deregulation. | as an important step toward free access and, thus, impact of goldenrice, a freedom-to-operate situation has been achieved for developing countries for the technology involved. specifically, to carry the invention beyond its initial "proof-of-concept" status in a japonica rice (oryza sativa) cultivar, we report here on two transformed elite indica varieties (ir64 and mtl250) plus one japonica variety taipei 309. indica varieties are predominantly consumed in the areas with vitamin a deficiency. t ... | 2003 | 12970483 |
| molecular cloning and functional analysis of a novel type of bowman-birk inhibitor gene family in rice. | bowman-birk inhibitor (bbi) genes encode serine protease inhibitors that have repetitive cysteine-rich domains with reactive sites for the trypsin or chymotrypsin family. we have identified seven bbi genes from japonica rice (oryza sativa subsp. japonica var teqing). all of the genes identified were found in a single cluster on the southern end of the long arm of rice chromosome 1. four of the seven bbi genes have two repetitive cysteine-rich domains, whereas one has a truncated domain with only ... | 2003 | 12972663 |