Publications

cloning, sequencing and expression of the s1 gene of avian reovirus.	the s1 genome segment of avian reovirus strain s1133 was cloned and completely sequenced. the sequence comprised 1636 bp with three distinct open reading frames (orfs), suggesting the gene was polycistronic in nature. the three orfs from 5' to 3' were predicted to encode polypeptides of 9.8, 3.8 and 34.9 kda, respectively. of the three orfs, only the third possessed the aug initiation codon in an optimum context for translation. the third orf-encoded protein, 326 amino acids in length, was expre ...	1995	7782781
protein coding assignment of avian reovirus strain s1133.	avian reovirus s1133 encodes 10 primary translation products, 8 of which are structural components of the viral particle and 2 of which are nonstructural proteins. the identity of the gene that codes for each of these polypeptides was determined by in vitro translation of denatured individual genome segments.	1994	8084013
production and characterization of monoclonal antibodies against avian reovirus strain s1133.	monoclonal antibodies (mabs) were produced against the avian reovirus strain s1133. mabs were isotyped and used to develop diagnostic tests. splenocytes from immunized mice were screened by enzyme-linked immunosorbent assay (elisa). two hybridomas secreted mabs against avian reovirus s1133. one mab secreted igg1 and the other secreted igg2a. all mab light chains were kappa specificity of mabs was tested against four avian reovirus strains: s1133, 1733, co8, and 2408. strains s1133, 1733, and 240 ...	1996	8790885
sequential partially overlapping gene arrangement in the tricistronic s1 genome segments of avian reovirus and nelson bay reovirus: implications for translation initiation.	previous studies of the avian reovirus strain s1133 (arv-s1133) s1 genome segment revealed that the open reading frame (orf) encoding the final sigmac viral cell attachment protein initiates over 600 nucleotides distal from the 5' end of the s1 mrna and is preceded by two predicted small nonoverlapping orfs. to more clearly define the translational properties of this unusual polycistronic rna, we pursued a comparative analysis of the s1 genome segment of the related nelson bay reovirus (nbv). se ...	2002	11752152
the avian reovirus genome segment s1 is a functionally tricistronic gene that expresses one structural and two nonstructural proteins in infected cells.	the avian reovirus s1 gene contains three partially overlapping, out-of-phase open reading frames (orfs) that the highly conserved in all avian reovirus strains examined to date. the three s1 orfs of the avian reovirus strain s1133 were individually expressed in bacterial cells, and their purified translation products used as antigens to raise specific polyclonal antibodies. with these antibodies we were able to demonstrate that all three s1 orfs from different avian reovirus strains are transla ...	2001	11883183
characterization of monoclonal antibodies against avian reovirus strain s1133.	monoclonal antibodies (mabs) were produced against a vaccinal s1133 strain of avian reovirus. characterization of six mabs in western blotting, radioimmunoprecipitation and gold immunoelectron microscopy revealed that the mabs were specific to the outer capsid proteins, mu2/mu2c, sigma2 and sigma3. two of three mabs, directed against sigma2 protein, neutralized the virus infectivity in a broadly specific manner, whereas the third had no neutralizing activity. the only mab which reacted with sigm ...	1996	18645837
interaction of avian reovirus with chicken lymphoblastoid cell lines.	four chicken lymphoblastoid cell lines were inoculated with avian reovirus strain s1133 and two local isolates, 965 and 615. of the inoculated cell lines, tlt, a b-cell line, was productively infected with the three viruses as demonstrated by immunofluorescence assay (ifa) and radioimmunoprecipitation assay. a comparative growth curve analysis of the three avian reoviruses was done at 37 degrees and 41 degrees c. isolate 965 replicated to a higher titre at both temperatures while the replication ...	1994	18671093
proteomics analysis of the df-1 chicken fibroblasts infected with avian reovirus strain s1133.	avian reovirus (arv) is a member of the orthoreovirus genus in the reoviridae family. it is the etiological agent of several diseases, among which viral arthritis and malabsorption syndrome are the most commercially important, causing considerable economic losses in the poultry industry. although a small but increasing number of reports have characterized some aspects of arv infection, global changes in protein expression in arv-infected host cells have not been examined. the current study used ...	2014	24667214