Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| development of resistance to amphotericin b in candida lusitaniae infecting a human. | candida lusitaniae associated with infection in a patient with acute myelogenous leukemia developed resistance to amphotericin b during systemic treatment of the patient. the organism, when isolated initially, was inhibited by 0.31 mug of amphotericin b per ml in yeast nitrogen base agar, but when isolated (20 days later) just antemortem and postmortem, required 100 and 50 mug/ml, respectively, for complete inhibition at 48 h. | 1979 | 290351 |
| identification of candida lusitaniae as an opportunistic yeast in humans. | four yeast strains, causally associated with infection in a patient with acute myelogenous leukemia, were identified by standard methods currently used in yeast taxonomy as representatives of candida lusitania van uden et do carmo-sousa. because this species has not been recognized previously as an opportunistic yeast in humans, molecular taxonomic methods were applied to confirm its identity. the nuclear deoxyribonucleic acid (dna) base composition of two clinical isolates was shown to be 45.1 ... | 1979 | 292646 |
| clavispora, a new yeast genus of the saccharomycetales. | in candida lusitaniae van uden et do carmo-sousa (1959), strains of opposite sex have been found. cells of the opposite mating types conjugate and form asci with one to four clavate spores. these are easily liberated from the ascus. the type strain of candida obtusa (dietrichson) van uden et do carmo-sousa ex van uden et buckley (1970) also produces ascospores after mating with one of the strains of candida lusitaniae. as clavate ascospores are unknown in yeasts, a new genus, clavispora, is prop ... | 1979 | 554535 |
| in vitro susceptibility of 245 yeast isolates to amphotericin b, 5-fluorocytosine, ketoconazole, fluconazole and itraconazole. | the in vitro activity of amphotericin b, 5-fluorocytosine, ketoconazole, fluconazole and itraconazole was tested against 245 yeast strains isolated from clinical specimens (68 candida albicans, 74 candida tropicalis, 43 candida krusei, 28 candida glabrata, 19 candida parapsilosis, 8 candida lusitaniae and 5 candida guilliermondii). an agar dilution method was employed to carry out testing. minimal inhibitory concentrations to restrain 90% of isolate growth (mic90) ranged from 0.12 to 2 mg/l for ... | 1992 | 1337508 |
| epidemiology of nosocomial acquisition of candida lusitaniae. | candida species are important nosocomial pathogens; however, little is known about the epidemiology of candida lusitaniae, an organism frequently resistant to amphotericin b. we evaluated 98 patients admitted to the bone marrow transplant and medical intensive care units of a tertiary-care hospital. each patient with c. lusitaniae was matched with control patients. restriction fragment analysis of dna was used to determine strain relatedness. seven patients (7.1%) with c. lusitaniae were identif ... | 1992 | 1360476 |
| candida lusitaniae infection in the newborn: case report and review of the literature. | 1992 | 1408490 | |
| strain delineation and epidemiology of candida (clavispora) lusitaniae. | electrophoretic karyotype (ek) patterns, determined by using contour-clamped homogeneous pulsed-field electrophoresis, and isoenzyme (iz) profiles were evaluated as methods for strain delineation among 35 isolates of candida lusitaniae recovered from 15 patients. all isolates were identified to the species level by using conventional morphologic and physiologic criteria, and the identification was confirmed by gas-liquid chromatography analysis of the cellular fatty acids. the isolates were then ... | 1992 | 1537915 |
| comparison study of broth macrodilution and microdilution antifungal susceptibility tests. | an evaluation of broth dilution antifungal susceptibility tests was performed by determining both the micro- and macrodilution mics of amphotericin b, flucytosine, fluconazole, ketoconazole, and cilofungin against 38 isolates of candida albicans, candida lusitaniae, candida parapsilosis, candida tropicalis, cryptococcus neoformans, and torulopsis glabrata. the following preliminary antifungal working group recommendations of the national committee for clinical laboratory standards for broth macr ... | 1991 | 1864923 |
| phylogenetic analysis of five medically important candida species as deduced on the basis of small ribosomal subunit rna sequences. | the classification of species belonging to the genus candida berkhout is problematic. therefore, we have determined the small ribosomal subunit rna (srrna) sequences of the type strains of three human pathogenic candida species; candida krusei, c. lusitaniae and c. tropicalis. the srrna sequences were aligned with published eukaryotic srrna sequences and evolutionary trees were inferred using a matrix optimization method. an evolutionary tree comprising all available eukaryotic srrna sequences, ... | 1991 | 1865186 |
| [susceptibility to miconazole (base) of isolates from the oral cavity and esophagus of patients with mycosis]. | an antifungal agent of the imidazole class, miconazole (mcz) (base), was investigated for its in vitro antifungal activities against isolates from the oral cavity and esophagus of patients with mycosis, and the following findings were obtained: 1. those yeast-like fungi which were presumed to be the causative agents were isolated from the oral cavity and esophagus of patients with mycosis, and identified. candida albicans was the most frequently occurring species, accounting for 71.4% of all the ... | 1991 | 1880917 |
| peritonitis caused by candida lusitaniae in patient on continuous ambulatory peritoneal dialysis (capd) | 1991 | 1889152 | |
| evaluation of the microscan rapid yeast identification panel. | the microscan rapid yeast identification (ryi) panel is a 4-h microdilution system for identification of clinical yeastlike isolates. its accuracy was evaluated by using 357 isolates encompassing 11 genera and 30 species. the ryi panel identifications were compared with those obtained by the api 20c system assisted with morphological characterization on cornmeal-tween 80 agar. the panels were read both visually and with the autoscan-4, a computer-controlled microplate reader. both the ryi panel ... | 1991 | 1939587 |
| in vitro susceptibility of 119 yeast isolates to fluconazole, 5-fluorocytosine, amphotericin b and ketoconazole. | the in vitro activity of fluconazole was tested against 13 yeast species (119 strains) isolated from clinical specimens during a 3-month period. for comparative purposes, three other antifungal compounds (5-fluorocytosine, amphotericin b and ketoconazole) were also tested. the tests were carried out using a microautomated method previously developed in our laboratory. the method allowed us to determine the minimum inhibitory concentration (mic) of the four antifungal drugs used. for each of the ... | 1991 | 2013239 |
| [fungal peritonitis during continuous ambulatory peritoneal dialysis (capd)]. | we report three cases of fungal peritonitis in patients undergoing capd, representing 1.3% of 228 episodes recorded during an 8 year period. the three patients, who had been catheterized for a long time, had previous episodes of bacterial peritonitis and had received antibiotic therapy. the culture was decisive to make the diagnosis. the change of the catheter and the treatment with antifungal agents contributed to cure the infection, although the three patients died from other causes. one case ... | 1991 | 2029556 |
| fermentation and aerobic metabolism of cellodextrins by yeasts. | the fermentation and aerobic metabolism of cellodextrins by 14 yeast species or strains was monitored. when grown aerobically, candida wickerhamii, c. guilliermondii, and c. molischiana metabolized cellodextrins of degree of polymerization 3 to 6. c. wickerhamii and c. molischiana also fermented these substrates, while c. guilliermondii fermented only cellodextrins of degree of polymerization less than or equal to 3. debaryomyces polymorphus, pichia guilliermondii, clavispora lusitaniae, and one ... | 1991 | 2039228 |
| [fatal catheter septicemia with finding of candida lusitaniae]. | a case of lethal pulmonary infection after catheter septicaemia with demonstration of c. lusitaniae in the blood is reported in a 73 year old patient with terminal crohn's disease receiving long-term parenteral nutrition. | 1990 | 2106744 |
| transport of glucose and cellobiose by candida wickerhamii and clavispora lusitaniae. | the cellular location of beta-1,4-glucosidase activity from, as well as the transport of glucose and cellobiose into, cells of clavispora lusitaniae nrrl y-5394 and candida wickerhamii nrrl y-2563 was investigated. the beta-glucosidase from cl. lusitaniae appeared to be a soluble cytoplasmic enzyme. this yeast transported both glucose and cellobiose when grown in medium containing cellobiose as the sole carbon source. glucose, but not cellobiose, uptake was observed for cells grown on glucose. t ... | 1990 | 2115884 |
| occurrence of clavispora lusitaniae, the teleomorph of candida lusitaniae, among clinical isolates. | of 13 clinical isolates of candida lusitaniae from diverse geographical regions, 7 represented the mating types (6 alpha, 1 a) of the ascomycete clavispora lusitaniae. selected nonfertile isolates showed significant dna relatedness (greater than 90%) to representatives of both mating types. phenotypic physiological characteristics, such as cellobiose fermentation and rhamnose assimilation, proved insufficient for separation of clavispora lusitaniae and clavispora opuntiae. | 1990 | 2229346 |
| evaluation of the updated vitek yeast identification data base. | using 398 isolates of yeasts and yeastlike fungi comprising 9 genera and 26 species, as well as the hyphomycete geotrichum candidum and the achlorophyllous alga prototheca wickerhamii, we compared the api 20c yeast identification system with the modified vitek yeast identification system with an expanded data base. we found 11 discrepancies between the two systems: five (1.3%) of the isolates (blastoschizomyces capitatus, 1; candida albicans, 1; hansenula anomala, 1; rhodotorula minuta, 2) had b ... | 1990 | 2229376 |
| emergence of a new opportunistic pathogen, candida lusitaniae. | candida lusitaniae has been an infrequently reported opportunistic pathogen. most previously reported cases of serious infection caused by this organism have proven fatal and were associated with amphotericin resistance of the organism. we report two patients with hematologic malignancies undergoing cytotoxic chemotherapy who developed fungemia with this organism while they were granulocytopenic. the organisms isolated from each patient were fully susceptible and were treated successfully with a ... | 1989 | 2644291 |
| clindamycin enhances murine delayed-type hypersensitivity and anti-candidal activity. | the contact sensitivity response of mice to dinitrofluorobenzene (dnfb), as determined by an epicutaneous painting and subsequent ear challenge assay, was enhanced by clindamycin administration. the optimal augmentation effect of clindamycin required its simultaneous administration at the time of dnfb skin sensitization. clindamycin also was found to boost both in-vitro and in-vivo murine response to experimental infection with candida lusitaniae. intraperitoneal injection of 1-2 mg of the drug ... | 1989 | 2759921 |
| candida lusitaniae septicemia in a patient on extended home intravenous hyperalimentation. | this is a report of the successful therapy of a case of candida lusitaniae septicemia in a patient with short bowel syndrome who had been on home intravenous hyperalimentation for approximately 3 months when the infection developed. c. lusitaniae was isolated from multiple blood cultures taken over the course of 2 days. | 1987 | 3106613 |
| effect of buffers on testing of candida species susceptibility to flucytosine. | synthetic amino acid medium for fungi (saamf) is a totally defined, nutritionally adequate, macromolecule-free culture medium for fungi that is buffered with an organic weak acid-weak base pair: 2-(n-morpholino)-propanesulfonic acid (mops) and 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris). in 1984, it was reported that mops-tris in saamf antagonized the activity of flucytosine against candida albicans (d. l. calhoun and j. n. galgiani, antimicrob. agents chemother. 26:364-367, 1984). accordin ... | 1987 | 3294891 |
| uncommon yeastlike zoopathogens and commercial systems for their identification. | opportunistic infections caused by yeasts or yeastlike fungi have increased in incidence in recent years as a result of clinical and therapeutic factors. several formerly uncommon yeastlike zoopathogens--candida lusitaniae, candida paratropicalis (sucrose-negative variant of candida tropicalis), trichosporon beigelii, blastoschizomyces capitatus, and rhodotorula species--have been isolated from patients with invasive infections. the increased isolation of such opportunistic pathogens from a vari ... | 1987 | 3309664 |
| candida lusitaniae: an emerging human pathogen. | 1987 | 3313235 | |
| candida lusitaniae: sepsis and meningitis in a neonate. | 1987 | 3313236 | |
| mycoses caused by candida lusitaniae. | candida lusitaniae, a fungus with a low incidence of infection in immunocompetent people, is emerging as an opportunistic pathogen in immunocompromised hosts. this yeast is generally resistant to amphotericin b and may present therapeutic difficulties. c. lusitaniae may be misidentified as one of several other fungal species, including candida tropicalis, candida parapsilosis, and even saccharomyces cerevisiae. as judged by in vitro antifungal susceptibility testing, minimal inhibitory concentra ... | 1987 | 3317728 |
| comparative activity in different media of ketoconazole, miconazole and amphotericin b against candida lusitaniae and sucrose-negative candida tropicalis. | this study evaluates the susceptibility of sucrose-negative candida tropicalis and candida lusitaniae strains to amphotericin b (amb), miconazole (mcz) and ketoconazole (ktz). the susceptibility tests were carried out in different media: antibiotic medium 3 (am-3m) and earle minimum essential medium (e-mem) for amb: yeast nitrogen base (ynb) and e-mem for imidazole compounds. the minimal fungicidal concentrations (mfcs) of amb were slightly higher than minimal inhibitory concentration (mics) exc ... | 1988 | 3378274 |
| postneurosurgical candida lusitaniae meningitis. | 1988 | 3380591 | |
| candida lusitaniae--an opportunistic pathogen. | candida lusitaniae is a newly recognized opportunistic pathogen amongst immunocompromised patients. the epidemiology and pathology remain largely unknown. this case report describes a 27-year-old female with vasculitis who acquired a fatal c. lusitaniae fungemia. | 1985 | 3967474 |
| transient fungemia due to candida lusitaniae. | 1985 | 3992314 | |
| in vitro susceptibilities of sucrose-negative candida tropicalis, candida lusitaniae, and candida norvegensis to amphotericin b, 5-fluorocytosine, miconazole, and ketoconazole. | the mics and minimal lethal concentrations of four antimycotics, amphotericin b, 5-fluorocytosine, miconazole nitrate, and ketoconazole, were determined for 25 yeast isolates representing species uncommonly implicated in candidiasis. a microdilution procedure was employed with complex and synthetic media. the isolates, in general, were susceptible to the same antimicrobial agents shown to be effective against candida albicans, but differences between some of the species in relative susceptibilit ... | 1984 | 6325491 |
| candida lusitaniae: frequency of recovery, colonization, infection, and amphotericin b resistance. | candida lusitaniae recovered from 58 specimens from 13 patients represented less than 1% of the yeasts isolated over a 15-month period. the majority of isolates were recovered from respiratory tract, stool, and urine specimens. of the 13 patients, 1 had a documented infection associated with septicema. urine isolates from that patient developed resistance to amphotericin b during therapy. | 1984 | 6520227 |
| fatal septicemia due to amphotericin b-resistant candida lusitaniae. | five yeast strains, causally associated with septicemia and death in a patient after peritonitis, were identified as candida lusitaniae van uden et do carmo-sousa by standard methods. the organism was initially susceptible to 5-fluorocytosine but strongly resistant to amphotericin b, requiring 50 micrograms/ml for complete inhibition at 48 h. | 1983 | 6619295 |
| candida lusitaniae: a new opportunistic pathogen of the urinary tract. | a 78-year-old man with mild diabetes presented with dysuria and frequency of urination of 15 months' duration subsequent to urinary catheterization for cataract surgery. multiple urine specimens revealed the presence of considerable quantities of yeast that were later identified as candida lusitaniae. the patient responded well to irrigation of the bladder with amphotericin b. | 1984 | 6713809 |
| comparative evaluation of the iatron serological candida check kit and the api 20c kit for identification of medically important candida species. | a newly developed commercial serological test (iatron laboratories, inc., tokyo, japan) for the rapid identification of medically important species of candida was evaluated against the api 20c (analytab products, plainview, n.y.) and the standard wickerham assimilation and fermentation procedures. our results indicated that the iatron and the api 20c methods are 95% accurate since both permitted identification of 78 of 82 candida isolates, representing eight medically important species. none of ... | 1981 | 7016900 |
| in vitro antimycotic sensitivity of yeasts isolated from infected bovine mammary glands. | a total of 91 yeast cultures isolated from infected bovine mammary glands in several herds were studied in vitro to determine their sensitivity to 7 antimycotic agents. yeast cultures were most sensitive to clotrimazole followed by ketoconazole, nystatin, myxin, miconazole, and amphotericin b and least sensitive to 5-fluorocytosine. the isolants represented 18 species. candida lusitaniae was most sensitive (85.7%) and c rugosa was least sensitive (31.9%) to the 7 antimycotic agents. | 1980 | 7212432 |
| [experimental pathogenicity of candida lusitaniae in mice]. | experimental pathogenicity was for the first time demonstrated in a strain of c. lusitaniae of clinical origin by the use of concentrated inocula (5.10(7) y-shaped cells) and the administration of 4 mg of methylprednisolone acetate i.m. injected 4 days before and 3 days after the inoculation. counts on malt-agar (fig. 1) in kidney and brain showed a decrease during the first 12 hours, followed by a definite increment in cell number between the first and second day. clinical tests showed a strong ... | 1981 | 7295397 |
| nadh dehydrogenase subunit genes in the mitochondrial dna of yeasts. | the genes encoding the nadh dehydrogenase subunits of respiratory complex i have not been identified so far in the mitochondrial dna (mtdna) of yeasts. in the linear mtdna of candida parapsilosis, we found six new open reading frames whose sequences were unambiguously homologous to those of the genes known to code for nadh dehydrogenase subunit proteins of different organisms, i.e., nd1, nd2, nd3, nd4l, nd5, and nd6. the gene for nd4 also appears to be present, as judged from hybridization exper ... | 1994 | 7521869 |
| inhibition of fungal growth by pseudomonas aeruginosa and pseudomonas cepacia isolated from patients with cystic fibrosis. | this study was undertaken because of the infrequency of infections due to candida species in patients with cystic fibrosis despite their extensive treatment with broad-spectrum antibiotics. in vitro susceptibility studies revealed significant inhibition of 11 strains of fungi known to infect human beings by 10 strains of pseudomonas aeruginosa and nine strains of pseudomonas cepacia isolated from the sputum of patients with cystic fibrosis. the fungi were candida krusei, candida keyfr, candida g ... | 1994 | 7522262 |
| comparison of four dna-based methods for strain delineation of candida lusitaniae. | four methods for the accurate delineation of epidemiologically related and unrelated strains of candida lusitaniae were compared. three pulsed-field electrophoretic methods, including two contour-clamped homogeneous field gel electrophoresis methods (ekp-1 and ekp-2) yielding electrophoretic karyotype patterns of intact chromosomal dna and a method in which the chromosomal dna was macrodigested with the endonuclease sfii prior to pulsed-field electrophoresis (mdp), and a random amplified polymor ... | 1995 | 7650168 |
| activities of d0870, a novel triazole, against candida lusitaniae and trichosporon beigelii in experimental murine infections. | candida lusitaniae and trichosporon beigelii may cause life-threatening infections in the immunocompromised host and may be resistant to amphotericin b. we assessed the activities of a new triazole, d0870, against one t. beigelii and four c. lusitaniae strains, in comparison with those of fluconazole and amphotericin b. immunosuppressed cf1 mice, intravenously infected with each fungal strain, received 3 days of therapy with oral d0870 (5 or 25 mg/kg of body weight daily), fluconazole (5 to 50 m ... | 1995 | 7726538 |
| epithelial adhesion in yeast species: correlation with surface expression of the integrin analog. | epithelial adhesion and expression of the integrin analog, a putative candidal adhesion, were correlated for 33 clinical and laboratory isolates of candida albicans, other candida species, and saccharomyces cerevisiae. on flow cytometry with saturating concentrations of the monoclonal antibody okm1, surface fluorescence was highest for c. albicans at 67.8% +/- 1.7% and significantly reduced for candida tropicalis (32.0% +/- 2.6%), candida parapsilosis (18.3% +/- 2.4%), candida glabrata (3.3% +/- ... | 1995 | 7769314 |
| comparative study of broth macrodilution and microdilution techniques for in vitro antifungal susceptibility testing of yeasts by using the national committee for clinical laboratory standards' proposed standard. | a comparative study of broth macro- and microdilution methods for susceptibility testing of fluconazole, itraconazole, flucytosine, and amphotericin b was conducted with 273 yeasts. the clinical isolates included 100 candida albicans, 28 candida tropicalis, 25 candida parapsilosis, 15 candida lusitaniae, 15 candida krusei, 50 cryptococcus neoformans var. neoformans, 25 torulopsis (candida) glabrata, and 15 trichosporon beigelii strains. both methods were performed according to the national commi ... | 1994 | 7814488 |
| efficacy of escalating doses of liposomal amphotericin b (ambisome) against hematogenous candida lusitaniae and candida krusei infection in neutropenic mice. | immunosuppressed cf1 mice were infected intravenously with two strains of candida krusei and four strains of candida lusitaniae (two of which were resistant to amphotericin b). mice were treated with 1 or 2 mg of amphotericin b desoxycholate per kg of body weight per day or escalating doses of liposomal amphotericin b (8 to 30 mg/kg/day) or were left untreated. higher doses of liposomal amphotericin b were as effective as standard dose of amphotericin b desoxycholate in prolonging survival but w ... | 1994 | 7872764 |
| strain delineation and antifungal susceptibilities of epidemiologically related and unrelated isolates of candida lusitaniae. | candida lusitaniae is an important nosocomial pathogen that may express resistance to one or more antifungal agents including amphotericin b. we investigated the genotypic diversity and antifungal susceptibility among 47 clinical isolates from 33 patients hospitalized in 12 different medical centers. strain delineation was performed by restriction endonuclease analysis of genomic dna (reag) with the restriction enzyme sfi i followed by pulsed-field gel electrophoresis and by electrophoretic kary ... | 1994 | 7874879 |
| evaluation of the albicans idr plate method for the rapid identification of candida albicans. | albicans idr is a ready-to-use medium containing a hexosaminic substrate that allows rapid and specific identification of candida albicans. in this study, we have evaluated its performance using 330 pure cultures of yeast strains previously identified by conventional mycological methods. the test correctly identified all the candida albicans strains tested (186 isolates), but there were 28 false-positive reactions after 48 h of culture incubation. the false-positive isolates corresponded to the ... | 1993 | 7935574 |
| infections due to resistant candida species in patients with cancer who are receiving chemotherapy. | the apparent increase in the prevalence of candidal infections among patients with cancer and the changes in the spectrum of candida pathogens infecting these patients are associated with a number of factors. selection of less-susceptible organisms occurs because antibacterial and antifungal agents alter microbial flora, and the use of these agents can lead to mutations that confer antibiotic resistance to initially susceptible organisms. candida organisms that are resistant to polyenes and azol ... | 1994 | 7948571 |
| mating in the heterothallic haploid yeast clavispora opuntiae, with special reference to mating type imbalances in local populations. | mating was studied in the haploid, heterothallic yeast clavispora opuntiae to assess the importance of nutritional, genetic, and other factors that may favour mating and recombination. local populations of this yeast generally exhibit dramatic inequalities in mating type distributions, suggesting that mating is rare in nature even though most isolates mate freely in the laboratory. the absence of assimilable nitrogen is prerequisite to mating competence, presumably by causing g1 arrest. maximum ... | 1994 | 7985417 |
| evaluation of two commercialized systems for the rapid identification of medically important yeasts. | a total of 77 recent clinical isolates of candida albicans and other medically important yeasts were identified by two different commercial tests, rapidec albicans (api-biomérieux) and fongiscreen 4h (sanofi diagnostics pasteur), and conventional mycological methods. the strains were from 13 different species of yeasts and consisted of strains of 36 c. albicans, three of candida famata, nine of candida (torulopsis) glabrata, five of candida guilliermondii, two of candida kefyr, three of candida ... | 1993 | 8015561 |
| correlation between in vitro and in vivo activity of antifungal agents against candida species. | the correlation between antifungal susceptibility testing and in vivo response to antifungal therapy was examined in experimental murine candidiasis. in vitro susceptibility testing was done using a microbroth dilution method. twenty-two candida albicans, 4 candida lusitaniae, and 2 candida krusei isolates were tested against fluconazole, flucytosine, and amphotericin b. in vivo antifungal activity was tested in murine hematogenous candidiasis. normal cf1 mice were infected with each of the c. a ... | 1994 | 8035024 |
| rapid presumptive identification of medically relevant yeasts to the species level by polymerase chain reaction and restriction enzyme analysis. | a method for the rapid presumptive differentiation of a panel of 12 clinically relevant yeasts to the species level was developed on the basis of evaluation by the polymerase chain reaction (pcr) of the gene coding for the small ribosomal subunit 18s-rrna. the method involved restriction enzyme analysis of pcr products obtained with primers common to all fungi. using six restriction enzymes, alui, bani, bbsi, draii, eco147i and nhei, characteristic pcr-restriction enzyme patterns were obtained f ... | 1994 | 8064542 |
| suppression of fungal growth exhibited by pseudomonas aeruginosa. | three surgery patients were monitored postoperatively, with particular reference to lung infection. in each case there was a clinical impression that pseudomonas aeruginosa suppressed the growth of candida albicans in patients with clinically significant lung infections from whom both of these organisms were isolated from serial sputum samples. regrowth of c. albicans after p. aeruginosa eradication occurred in two patients, despite fluconazole therapy, to which both c. albicans isolates were su ... | 1994 | 8150966 |
| [fatal outcome of pneumocystis-carinii pneumonia under low-dose methotrexate and prednisone therapy for chronic rheumatoid arthritis. case report and literature review]. | new therapeutic schemes have been proposed recently for treatment of rheumatoid arthritis. among these, methotrexate at low doses is recommended as basic therapy, already shortly after the diagnosis is established. we report on a 68-year-old female with rheumatoid arthritis who developed bilateral pneumocystis-carinii pneumonia with a lethal course and bilateral sinusitis maxillaris with candida lusitaniae. various opportunistic infections have been described in the literature after low-dose met ... | 1994 | 8184239 |
| candida lusitaniae causing fatal meningitis. | fatal meningitis due to candida lusitaniae in a 35 year old previously healthy man is described. c. lusitaniae is an opportunistic fungal pathogen reported infrequently in the english literature. this is the third case report of meningitis and the first fatal infection in an adult from central india due to c. lusitaniae known to the authors. | 1993 | 8290437 |
| comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for torulopsis glabrata and candida species other than c. albicans. | candida species have recently emerged as important nosocomial pathogens. because of the lack of a reliable system for detecting differences within the same species, little is known about the epidemiology of infection with candida species. we describe a typing system for torulopsis glabrata and the non-c. albicans candida species that uses contour-clamped homogeneous electric field electrophoresis (chef), a version of pulsed-field gradient gel electrophoresis, and compared it with restriction enz ... | 1993 | 8396585 |
| candida lusitaniae osteomyelitis in a premature infant. | candida lusitaniae is emerging as an opportunistic pathogen in premature infants. treatment of c. lusitaniae is challenging due to frequent resistance of this organism to antifungal agents and lack of standardized susceptibility testing for fungi. we report the case of a premature infant with c. lusitaniae osteomyelitis in whom treatment was successful with 5-fluorocytosine and fluconazole. | 1993 | 8397571 |
| multicenter evaluation of a broth macrodilution antifungal susceptibility test for yeasts. | thirteen laboratories collaborated to optimize interlaboratory agreement of results of a broth macrodilution procedure for testing three classes of antifungal drugs against pathogenic yeasts. the activities of amphotericin b, flucytosine, and ketoconazole were tested against 100 coded isolates of candida albicans, candida tropicalis, candida parapsilosis, candida lusitaniae, torulopsis (candida) glabrata, and cryptococcus neoformans. two starting yeast inoculum sizes (5 x 10(4) and 2.5 x 10(3) c ... | 1993 | 8431016 |
| efficacy and tolerance of an amphotericin b lipid (intralipid) emulsion in the treatment of candidaemia in neutropenic patients. | the efficacy and tolerance of a new amphotericin b lipid emulsion (amb-il) in which amphotericin b was diluted in a lipid solution for parenteral nutrition (intralipid) was assessed in fourteen episodes of candidaemia occurring in neutropenic patients. the strains isolated were candida krusei (nine cases), candida albicans (three cases), candida parapsilosis (one case) and candida lusitaniae (one case). an amb-il was administered at a mean dosage of 1.18 mg/kg/day (range 0.73-1.55) for 22 days ( ... | 1993 | 8444659 |
| antifungal susceptibility testing of yeasts: evaluation of technical variables for test automation. | the technical parameters for antifungal susceptibility testing with candida species were reexamined to determine the optimal conditions for testing with semiautomated preparations of broth microdilution cultures, automated spectrophotometric readings of the cultures, and dose-response and endpoint determinations by means of a computer spreadsheet. tests were based on proposed standard method m27p of the national committee for clinical laboratory standards for antifungal agents. rpmi 1640 broth w ... | 1995 | 8540715 |
| yeast communities in a natural tequila fermentation. | fresh and cooked agave, drosophila spp., processing equipment, agave molasses, agave extract, and fermenting must at a traditional tequila distillery (herradura, amatitan, jalisco, méxico) were studied to gain insight on the origin of yeasts involved in a natural tequila fermentations. five yeast communities were identified. (1) fresh agave contained a diverse mycobiota dominated by clavispora lusitaniae and an endemic species, metschnikowia agaveae. (2) drosophila spp. from around or inside the ... | 1995 | 8546452 |
| comparative evaluation of macrodilution and alamar colorimetric microdilution broth methods for antifungal susceptibility testing of yeast isolates. | a comparative evaluation of the macrodilution method and the alamar colorimetric method for the susceptibility testing of amphotericin b, fluconazole, and flucytosine was conducted with 134 pathogenic yeasts. the clinical isolates included 28 candida albicans, 17 candida tropicalis, 15 candida parapsilosis, 12 candida krusei, 10 candida lusitaniae, 9 candida guilliermondii, 18 torulopsis glabrata, and 25 cryptococcus neoformans isolates. the macrodilution method was performed and interpreted acc ... | 1995 | 8567901 |
| molecular probes for diagnosis of fungal infections. | we have developed 21 specific nucleic acid probes which target the large subunit rrna genes from aspergillus flavus, aspergillus fumigatus, aspergillus glaucus, aspergillus niger, aspergillus terreus, blastomyces dermatitidis, candida albicans, candida (torulopsis) glabrata, candida guilliermondii, candida kefyr, candida krusei, candida lusitaniae, candida parapsilosis, candida tropicalis, coccidioides immitis, cryptococcus neoformans var. gattii, cryptococcus neoformans var. neoformans, filobas ... | 1995 | 8576345 |
| use of chromagar candida medium for isolation of yeasts from dental samples. | a new differential medium, chromagar candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. the recovery of yeasts on the medium was not significantly different from the recovery on sabouraud dextrose agar. the identities of 450 green colonies on chromagar candida, presumptively identified as candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-n-acetylgalacto ... | 1995 | 8576366 |
| new and emerging yeast pathogens. | the most common yeast species that act as agents of human disease are candida albicans, candida tropicalis, candida glabrata, candida parapsilosis, and cryptococcus neoformans. the incidence of infections by other yeasts has increased during the past decade. the most evident emerging pathogens are malassezia furfur, trichosporon beigelii, rhodotorula species, hansenula anomala, candida lusitaniae, and candida krusei. organisms once considered environmental contaminants or only industrially impor ... | 1995 | 8665465 |
| evaluation of a commercial medium for identification of candida species. | chromagar candida (chromagar, france) was evaluated as a medium for the presumptive identification and isolation of yeasts using 1,537 isolates of medically important yeasts, including 970 candida albicans, 165 candida parapsilosis, 131 candida glabrata, 62 candida guilliermondii, 35 candida krusei, 32 candida tropicalis, 31 rhodotorula rubra, 23 trichosporon spp. (17 trichosporon beigelii), 17 candida famata, 16 candida pelliculosa, 10 pichia etchelsii, 10 saccharomyces cerevisiae, 8 candida lu ... | 1996 | 8801088 |
| comparison of isolator 1.5 and bactec nr660 aerobic 6a blood culture systems for detection of fungemia in children. | the isolator 1.5 microbial system (iso 1.5) (wampole laboratories, cranbury, n.j.) was compared with the bactec nr660 aerobic nr6a bottle (nr6a) (becton dickinson diagnostic instrument systems, sparks, md.) for the detection of fungemia in hospitalized pediatric patients. for 4,825 paired blood cultures evaluated retrospectively from april 1992 to december 1994, at least one blood culture system was positive for 89 clinically important fungal isolates involved in 36 episodes of fungemia in 34 pa ... | 1996 | 8818873 |
| activity of a new triazole, sch 56592, compared with those of four other antifungal agents tested against clinical isolates of candida spp. and saccharomyces cerevisiae. | sch 56592 is a new triazole agent with potent, broad-spectrum antifungal activity. the in vitro activities of sch 56592, itraconazole, fluconazole, amphotericin b, and flucytosine (5-fc) against 404 clinical isolates of candida spp. (382 isolates) and saccharomyces cerevisiae (22 isolates) were investigated. in vitro susceptibility testing was performed by a broth microdilution method performed according to national committee for clinical laboratory standards guidelines. overall, sch 56592 was v ... | 1997 | 9021172 |
| influence of l-galactonic acid gamma-lactone on ascorbate production in some yeasts. | l-galactonic acid gamma-lactone appear to influence ascorbic and production in strains of saccharomyces cerevisiae, clavispora lusitaniae, cryptococcus terreus, pichia fermentans in which this is undetected whenever glucose represents the sole carbon source. cryptococcus terreus (strains dbvp 6012 and 6242) does not show ascorbic acid production either in presence or in the absence of l-galactonic acid gamma-lactone. this feature is probably connected to the insensibility of the strain to the ly ... | 1997 | 9111923 |
| efficacy of continuous flucytosine infusion against candida lusitaniae in experimental hematogenous murine candidiasis. | candida lusitaniae may cause life-threatening infections in the immunocompromised host and may be resistant to amphotericin b. flucytosine (5-fc) is very active against c. lusitaniae isolates in vitro, while the in vivo response of murine infection to 5-fc is not as good. to evaluate the hypothesis that this discrepancy may be primarily due to the short half-life of 5-fc in mice, we compared the same total dosage of 75 mg of 5-fc per kg of body weight per day given by bolus injections or infused ... | 1996 | 9124867 |
| in-vitro susceptibility pattern of candida lusitaniae and evaluation of the etest method. | the antifungal susceptibility of 35 candida lusitaniae isolates was determined in vitro by the national committee for clinical laboratory standards (nccls) m27-p macrodilution methodology. all the isolates were susceptible to ketoconazole, itraconazole and fluconazole. of the 35 isolates, eight (23%) were resistant to flucytosine. for amphotericin b, m27-p yielded a narrow range of mics (0.06-0.5 mg/l). we therefore investigated the activity of this drug by reading mics at 72 h and by using alte ... | 1997 | 9184357 |
| in vitro antifungal activity of bms-181184 against systemic isolates of candida, cryptococcus, and blastomyces species. | bms-181184 is a water-soluble derivative of the pradimicin group of antifungal compounds. we determined the in vitro activities of bms-181184 and comparator agents amphotericin b, 5-fluorocytosine, fluconazole, and ketoconazole against 184 systemic fungal isolates collected at the health sciences centre in winnipeg, canada, between 1987 and 1995. bms-181184 demonstrated mics of between 1 and 8 micrograms/ml for all candida albicans, candida glabrata, candida tropicalis, candida krusei, candida l ... | 1997 | 9327245 |
| evaluation of the echinocandin antifungal mk-0991 (l-743,872): efficacies in mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis. | the in vivo activity of the merck antifungal echinocandin drug candidate mk-0991 (l-743,872) was evaluated in mouse models of disseminated candidiasis, aspergillosis, and cryptococcosis. the echinocandins are potent inhibitors of 1,3-beta-d-glucan synthase. two models of disseminated candidiasis were used. in a candida albicans mouse survival model with both dba/2n and cd-1 mice, estimates of the 50% effective doses (ed50s) of mk-0991 were 0.04 and 0.10 mg/kg of body weight/dose at 21 days after ... | 1997 | 9371329 |
| genetic characterization of a phospholipase c gene from candida albicans: presence of homologous sequences in candida species other than candida albicans. | phospholipase c (plc) enzymes are essential in regulating several important cellular functions in eukaryotes, including yeasts. in this study, pcr was used to identify a gene encoding plc activity in candida albicans, using oligonucleotide primers complementary to sequences encoding highly conserved amino acid regions within the x domains of previously characterized eukaryotic phospholipase c genes. the nucleotide sequence of the c. albicans gene, caplc1 (2997 bp), was determined from a recombin ... | 1998 | 9467900 |
| olecranon bursitis caused by infection with candida lusitaniae. | we describe a 59-year-old woman with diabetes and chronic asthma treated with prednisone and methotrexate who developed chronic olecranon bursitis caused by candida lusitaniae. infection, especially with unusual microbial pathogens, should be considered in cases of chronic bursitis in patients taking immunosuppressive medicine, even if the classic signs of septic bursitis are absent. infection with c. lusitaniae, a component of the normal mycoflora, may be a marker of serious immunosuppression, ... | 1998 | 9517788 |
| candida lusitaniae: an uncommon cause of prosthetic valve endocarditis. | 1998 | 9524867 | |
| nosocomial breakthrough fungaemia during antifungal prophylaxis or empirical antifungal therapy in 41 cancer patients receiving antineoplastic chemotherapy: analysis of aetiology risk factors and outcome. | forty-one episodes of breakthrough fungaemia occurring over a 7.5 year period in the national and st elizabeth's cancer institutes in bratislava, slovakia, were analysed. five of them occurred during prophylaxis with fluconazole (one torulopsis glabrata, one hansenula anomala, two candida krusei and one candida parapsilosis), ten with itraconazole (three trichosporon pullulans, one trichosporon beigelii, one cryptococcus laurentii, three candida albicans and two t. glabrata), 11 during prophylax ... | 1998 | 9578164 |
| in vitro activities of terbinafine against cutaneous isolates of candida albicans and other pathogenic yeasts. | terbinafine is active in vitro against a wide range of pathogenic fungi, including dermatophytes, molds, dimorphic fungi, and some yeasts, but earlier studies indicated that the drug had little activity against candida albicans. in contrast, clinical studies have shown topical and oral terbinafine to be active in cutaneous candidiasis and candida nail infections. in order to define the anti-candida activity of terbinafine, we tested the drug against 350 fresh clinical isolates and additional str ... | 1998 | 9593126 |
| evidence for person-to-person transmission of candida lusitaniae in a neonatal intensive-care unit. | candida lusitaniae is an increasingly important nosocomial bloodstream pathogen. epidemiological investigation and molecular typing techniques identified three neonates infected with identical strains of c lusitaniae that were distinguished readily from epidemiologically unrelated strains from other locations in the hospital. the results of this study provide evidence for nosocomial transmission of c lusitaniae in a neonatal intensive-care unit and suggest that these infants are at increased ris ... | 1998 | 9613696 |
| evaluation of the etest method for determining fluconazole susceptibilities of 402 clinical yeast isolates by using three different agar media. | the performance of the etest for fluconazole susceptibility testing of 402 yeast isolates was assessed against the national committee for clinical laboratory standards (nccls) microdilution broth method. the nccls method employed rpmi 1640 broth medium, and mics were read after incubation for 48 h at 35 degrees c. etest mics were determined with rpmi agar containing 2% glucose (rpg), casitone agar (cas), and mueller-hinton agar (mha) and were read after incubation for 48 h at 35 degrees c. the y ... | 1998 | 9705397 |
| fungemia in cancer patients in brazil: predominance of non-albicans species. | the objective of this study was to characterize the epidemiology of candidemia in cancer patients in the city of rio de janeiro, brazil. an 18-month survey of fungemia in patients with cancer was undertaken in three hospitals in rio de janeiro. forty-three episodes of candidemia were identified in 43 patients, 43 of which were episodes of candidemia; in ten case the strains were not available for further identification of species and were excluded from this analysis. the overall distribution of ... | 1998 | 9750336 |
| first isolation of trichophyton fischeri in the united states. | a 49-year-old male with pneumocystis carinii pneumonia was seen at bellevue hospital in new york, n.y. sputum samples yielded cultures of candida lusitaniae, mycobacterium avium, and a filamentous fungus, trichophyton fischeri. t. fischeri is a nonpathogenic fungus which resembles the dermatophyte trichophyton rubrum. this is the first record of the species from u.s. sources. this case exemplifies the ecological differences between t. fischeri and t. rubrum and illustrates how correct identifica ... | 1998 | 9774603 |
| comparison of a new commercial colorimetric microdilution method with a standard method for in-vitro susceptibility testing of candida spp. and cryptococcus neoformans. | the sensititre yeast one method (accumed international ltd, east grinstead, uk) is a microplate-based procedure that incorporates an oxidation-reduction indicator, alamar blue, for the in-vitro testing of five antifungal agents (amphotericin b, fluconazole, itraconazole, ketoconazole and flucytosine). we compared this colorimetric method with a standard broth microdilution test, performed according to us national committee for clinical laboratory standards document m27-a guidelines, for determin ... | 1998 | 9818741 |
| invasive infections due to clavispora lusitaniae. | three cases of clavispora lusitaniae invasive fungal infections are reported. all three infections appeared in cancer patients presented with fungaemia, one additionally with meningitis. two of them were breakthrough -- they developed during therapy with conventional amphotericin b with a dose of 0.5 mg kg(-1) day(-1) . all three were cured: two with intravenous fluconazol and one with an increasing dose (1 mg kg(-1) day(-1)) of amphotericin b. in one of two breakthrough cases the sensitivity of ... | 1999 | 10030550 |
| fungal peritonitis in patients on continuous ambulatory peritoneal dialysis. | the purpose of this study was to analyze the microbiological and clinical features of fungal peritonitis in patients with endstage renal failure treated with continuous ambulatory peritoneal dialysis (capd). the diagnosis of peritonitis was based on abdominal discomfort or pain, cloudy peritoneal effluent with an elevated leukocyte count and isolation of fungi from the peritoneal effluent. amphotericin b, flucytosine, ketoconazole, miconazole and more recently fluconazole were used for antifunga ... | 1998 | 10052546 |
| prospective study of nosocomial fungal meningitis in children--report of 10 cases. | within an 8-year period, 10 cases of fungal nosocomial meningitis in children 0-13 y old were prospectively identified, 3 caused by yeasts other than candida spp. (rhodotorula rubra, aureobasidium mansoni, clavispora lusitaniae) and 7 by candida albicans. seven patients survived. whereas 3 neonates with fungal meningitis (all due to c. albicans) died. risk factors for fungal nosocomial meningitis included cancer (2 children), previous neurosurgery (2 children), cranial trauma (1 case) and premat ... | 1998 | 10066050 |
| high-frequency, in vitro reversible switching of candida lusitaniae clinical isolates from amphotericin b susceptibility to resistance. | recent studies have revealed an increase in the incidence of serious infections caused by non-albicans candida species. candida lusitaniae is of special interest because of its sporadic resistance to amphotericin b (amb). the present in vitro study demonstrated that, unlike other candida species, c. lusitaniae isolates frequently generated amb-resistant lineages form previously susceptible colonies. cells switching from a resistant colony to a susceptible phenotype were also detected after treat ... | 1999 | 10103188 |
| invasive yeast infections other than candida spp. in acute leukaemia. | during the last two decades, the treatment of leukaemia has changed significantly; increasing intensity of chemotherapy and bone marrow transplantation have lead to profound immunosuppression, prolonged stays in hospital, vascular catheterization, administration of broad spectrum antimicrobial agents and extensive use of prophylactic antifungal drugs. all but the last of these risk factors have increased the incidence of fungal infections in leukaemic patients and have significantly changed the ... | 1999 | 10204120 |
| in-vitro activity of dicationic aromatic compounds and fluconazole against cryptococcus neoformans and candida spp. | we investigated the in-vitro activity of three selected dicationic aromatic compounds for nine clinical isolates of cryptococcus neoformans and 93 clinical isolates of candida spp., representing 12 different species, using a broth macrodilution method following nccls recommendations. all the clinical isolates were also tested for fluconazole susceptibility. the in-vitro data demonstrate that compounds 39 and 57 have excellent in-vitro activity for all tested strains (mic 0.19-1.56 mg/l) except c ... | 1999 | 10473229 |
| comparative in vitro activity of antiseptics and disinfectants versus clinical isolates of candida species. | to evaluate the in vitro activity of antiseptics and detergents against candida. | 1999 | 10530645 |
| species and genotypic diversities and similarities of pathogenic yeasts colonizing women. | we examined the patterns of strain relatedness among pathogenic yeasts from within and among groups of women to determine whether there were significant associations between genotype and host condition or body site. a total of 80 yeast strains were isolated, identified, and genotyped from 49 female volunteers, who were placed in three groups: (i) 19 women with aids, (ii) 11 pregnant women without human immunodeficiency virus (hiv) infection, and (iii) 19 women who were neither pregnant nor infec ... | 1999 | 10565893 |
| a head-on comparison of the in vitro antifungal activity of conventional and lipid-based amphotericin b: a multicenter study. | a comparative study of conventional amphotericin b, abelcet and ambisome was performed using a microdilution format of the nccls m27-a methodology for susceptibility testing against 300 fungal isolates (152 yeasts, 148 filamentous fungi) in both rpmi-1640 and antibiotic medium #3 (ab3). the clinical isolates included candida albicans (n=54), candida glabrata (n=25), candida parapsilosis (n=23), candida krusei (n=19), candida lusitaniae (n=14), cryptococcus neoformans (n=5), candida tropicalis (n ... | 2000 | 10768512 |
| [evaluation of a new method for antifungal drugs susceptibility testing to yeasts]. | we compared the etest with a broth microdilution method (fp panel), performed according to the national committee for modified clinical laboratory standards (nccls) document m27-p guidelines, for determining the mics of 81 clinical isolates of yeasts (7 candida albicans, 8 candida glabrata, 10 candida parapsilosis, 6 pichia anomala, 10 candida tropicalis, 4 candida guilliermondii, 4 candida krusei, 6 trichosporon cutaneum, 5 candida ciferrii, 3 candida famata, 4 candida norvegensis, 2 rhodotorul ... | 2000 | 10783576 |
| a ste12 homolog is required for mating but dispensable for filamentation in candida lusitaniae. | candida lusitaniae is a dimorphic yeast that is emerging as an opportunistic fungal pathogen. in contrast to candida albicans, which is diploid and asexual, c. lusitaniae has been reported to have a sexual cycle. we have employed genetic approaches to demonstrate that c. lusitaniae is haploid and has a sexual cycle involving mating between mata and matalpha cells under nutrient deprivation conditions. by degenerate pcr, we identified a c. lusitaniae homolog (cls12) of the ste12 transcription fac ... | 2000 | 10790381 |
| comparative evaluation of a commercial system for identification of candida lusitaniae. | the ability of the api candida system (biomérieux, france) to identify candida lusitaniae isolates was evaluated in comparison to the auxacolor and id 32c systems using 52 clinical isolates previously identified on the basis of their morphology and their biochemical reactions in the auxacolor and id 32c systems. the api candida system failed to definitively identify most of the strains tested within 24 h. no beta-maltosidase activity was detected in 28 strains, and supplementary tests were requi ... | 2000 | 10898145 |
| flow cytometry antifungal susceptibility testing of pathogenic yeasts other than candida albicans and comparison with the nccls broth microdilution test. | candida species other than candida albicans frequently cause nosocomial infections in immunocompromised patients. some of these pathogens have either variable susceptibility patterns or intrinsic resistance against common azoles. the availability of a rapid and reproducible susceptibility-testing method is likely to help in the selection of an appropriate regimen for therapy. a flow cytometry (fc) method was used in the present study for susceptibility testing of candida glabrata, candida guilli ... | 2000 | 10991856 |
| nosocomial candidemia in non-neutropenic patients at an italian tertiary care hospital. | in a retrospective study conducted in an italian tertiary care hospital, the incidence of nosocomial candidemia was evaluated together with causative pathogens, treatment, and risk factors for death. over a 6-year period (1992-1997), a total of 189 episodes of candidemia occurred in 189 patients (mean age 58+/-19 years), accounting for an average incidence of 1.14 episodes per 10,000 patient-days per year. the most common reasons for hospitalization were solid neoplasia (21%), trauma (17%), abdo ... | 2000 | 11014622 |
| improved detection of amphotericin b-resistant isolates of candida lusitaniae by etest. | both intrinsic and acquired resistance to amphotericin b have been documented for candida lusitaniae. amphotericin b remains the drug of choice for many critical fungal infections, and the detection of resistance is essential to monitor treatment effectively. the limitations of the national committee for clinical laboratory standards (nccls) reference methodology for detection of amphotericin b resistance are well documented, and several alternative methods have been proposed. etest assays with ... | 2001 | 11136795 |
| identification of rare candida species and other yeasts by polymerase chain reaction and slot blot hybridization. | invasive candidiasis has become a major cause of morbidity and mortality in immunocompromised hosts. here we describe a fast and reliable dna extraction and pcr amplification method in combination with a slot blot hybridization assay. a genus-specific probe was designed that allowed to detect dna from a broad range of candida species and 3 other yeasts. in addition, species-specific oligonucleotides for emerging candida and other yeast species allowed to identify dna extracted from candida lusit ... | 2000 | 11146245 |
| influence of glucose supplementation and inoculum size on growth kinetics and antifungal susceptibility testing of candida spp. | the influences of inoculum size and glucose supplementation on the growth kinetics of 60 candida spp. clinical isolates (candida albicans, candida tropicalis, candida parapsilosis, candida glabrata, candida krusei, and candida lusitaniae [10 isolates each]) are assessed. the combined influence of growth and reading method (visual or spectrophotometric) on the determination of the mics of amphotericin b, flucytosine, fluconazole, itraconazole, ketoconazole, and voriconazole is also analyzed, and ... | 2001 | 11158101 |