Publications

use of highly conserved motifs in plant virus rna polymerases as the tags for specific detection of carmovirus-related rna-dependent rna polymerase genes.	two highly degenerate primers for sequence-specific amplification and cloning of a 510-nucleotide-long segment of rna-dependent rna-polymerase (rdrp) genes were selected and synthesized on the basis of available plant carmovirus-like viral rdrp sequences. these primers were shown to be efficient in pcr screening of different rdrp genes including those of carmoviruses, dianthoviruses, and tombusviruses. in particular, they were used for amplification, cloning, and sequencing of an rdrp gene fragm ...	1995	7871745
relationship of the pelargonium flower break carmovirus (pfbv) coat protein gene with that of other carmoviruses.	the 3'-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (pfbv) were sequenced from rt-pcr amplification products. one large orf was found, encoding a 345 amino acid protein of mr 37 kda, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation. the sequence also included the putative promoter of the coat protein gene subgenomic rna, as well as its 5' and 3' untranslated regions. the pfbv coat pr ...	1998	9787665
characterization and in vitro translation analysis of pelargonium flower break virus.	pelargonium flower break virus (pfbv) is one of the common viruses in the glasshouses of western europe and has been assigned to the genus carmovirus. a spanish isolate obtained from nursery-grown pelargonium zonale plants (pfbv-m) has been characterized. the molecular weight of genomic rna and coat protein of pfbv-m were determined to be 1.36 x 10(6) (corresponding to approximately 4 kb) and 36,000, respectively. only genomic-size rna was encapsidated in pfbv virions; making necessary to purify ...	1999	10486116
complete nucleotide sequence and genome organization of pelargonium flower break virus.	the complete nucleotide sequence of pelargonium flower break virus (pfbv) has been determined. the genomic rna is 3923 nucleotides (nt) long and contains five open reading frames (orfs). the 5'-proximal orf encodes a 27 kda protein (p27) and terminates with an amber codon which may be read-through into an in-frame p56 orf to generate a 86 kda protein (p86) containing the viral rna dependent-rna polymerase motifs. two small orfs, located in the central part of the viral genome, encode polypeptide ...	2004	14991450
insights into the selective pressures restricting pelargonium flower break virus genome variability: evidence for host adaptation.	the molecular diversity of pelargonium flower break virus (pfbv) was assessed using a collection of isolates from different geographical origins, hosts, and collecting times. the genomic region examined was 1,828 nucleotides (nt) long and comprised the coding sequences for the movement (p7 and p12) and the coat (cp) proteins, as well as flanking segments including the entire 3' untranslated region (3' utr). some constraints limiting viral heterogeneity could be inferred from sequence analyses, s ...	2006	16873268
complete nucleotide sequence of nootka lupine vein-clearing virus.	the complete genome sequence of nootka lupine vein-clearing virus (nlvcv) was determined to be 4,172 nucleotides in length containing four open reading frames (orfs) with a similar genetic organization of virus species in the genus carmovirus, family tombusviridae. the order and gene product size, starting from the 5'-proximal orf consisted of: (1) polymerase/replicase gene, orf1 (p27) and orf1rt (readthrough) (p87), (2) movement proteins orf2 (p7) and orf3 (p9), and, (3) the 3'-proximal coat pr ...	2007	17657600
biological and molecular characterization of a novel carmovirus isolated from angelonia.	abstract a new carmovirus was isolated from angelonia plants (angelonia angustifolia), with flower break and mild foliar symptoms, grown in the united states and israel. the virus, for which the name angelonia flower break virus (anfbv) is proposed, has isometric particles, approximately 30 nm in diameter. the experimental host range was limited to nicotiana species, schizanthus pinnatus, myosotis sylvatica, phlox drummondii, and digitalis purpurea. virions were isolated from systemically infect ...	2006	18944305
inhibition of rna silencing by the coat protein of pelargonium flower break virus: distinctions from closely related suppressors.	viral-derived double-stranded rnas (dsrnas) activate rna silencing, generating small interfering rnas (sirnas) which are incorporated into an rna-induced silencing complex (risc) that promotes homology-dependent degradation of cognate rnas. to counteract this, plant viruses express rna silencing suppressors. here, we show that the coat protein (cp) of pelargonium flower break virus (pfbv), a member of the genus carmovirus, is able to efficiently inhibit rna silencing. interestingly, pfbv cp bloc ...	2009	19141463
characterization of the subgenomic rnas produced by pelargonium flower break virus: identification of two novel rnas species.	pelargonium flower break virus (pfbv), a member of the genus carmovirus, has a single-stranded positive-sense genomic rna (grna) of 3.9kb. the 5' half of the grna encodes two proteins involved in replication, the p27 and its readthrough product, p86 (the viral rna dependent-rna polymerase, rdrp), and the 3' half encodes two small movement proteins, p7 and p12, and the coat protein (cp). as other members of the family tombusviridae, carmoviruses express orfs that are not 5'-proximal from subgenom ...	2009	19428742
identification and characterization of rna-binding activity in the orf1-encoded replicase protein of pelargonium flower break virus.	pelargonium flower break virus (pfbv) belongs to the genus carmovirus (family tombusviridae) and, as with the remaining members of the group, possesses a monopartite genome of single-stranded, positive-sense rna that contains five orfs. the two 5'-proximal orfs (orfs 1 and 2) encode two polypeptides of 27 and 86 kda (p27 and p86), respectively, that show homology with replication proteins. the p27 does not present any motif to explain its presumed involvement in replication, while p86 has the mo ...	2010	20826617
a membrane-associated movement protein of pelargonium flower break virus shows rna-binding activity and contains a biologically relevant leucine zipper-like motif.	two small viral proteins (dgbp1 and dgbp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each protein partner are not yet clear. here we have confirmed the requirement of the dgbps of pelargonium flower break virus (pfbv), p7 and p12, for pathogen movement. studies focused on p12 have shown that it associates to cellular membranes, which is in accordance to its hydrophobic pro ...	2011	21444100
limited elimination of two viruses by cryotherapy of pelargonium apices related to virus distribution.	the possibility of eradicating the pelargonium flower break virus (pfbv) and pelargonium line pattern virus (plpv) by cryotherapy of axillary shoot apices was investigated using five pelargonium cultivars. viruses were detected by das-elisa and their location was determined by immunolocalization. apex culture did not permit elimination of pfbv and only 15 percent regenerated plants of 'stellar artic' cultivar were elisa plpv-negative. plants regenerated from cryotherapy-treated apices were teste ...	2011	21766140
an internal ribosome entry site directs translation of the 3'-gene from pelargonium flower break virus genomic rna: implications for infectivity.	pelargonium flower break virus (pfbv, genus carmovirus) has a single-stranded positive-sense genomic rna (grna) which contains five orfs. the two 5'-proximal orfs encode the replicases, two internal orfs encode movement proteins, and the 3'-proximal orf encodes a polypeptide (p37) which plays a dual role as capsid protein and as suppressor of rna silencing. like other members of family tombusviridae, carmoviruses express orfs that are not 5'-proximal from subgenomic rnas. however, in one case, c ...	2011	21818349
analysis of the subcellular targeting of the smaller replicase protein of pelargonium flower break virus.	replication of all positive rna viruses occurs in association with intracellular membranes. in many cases, the mechanism of membrane targeting is unknown and there appears to be no correlation between virus phylogeny and the membrane systems recruited for replication. pelargonium flower break virus (pfbv, genus carmovirus, family tombusviridae) encodes two proteins, p27 and its read-through product p86 (the viral rna dependent-rna polymerase), that are essential for replication. recent reports w ...	2011	22222362