Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [isolation of the bovine herpesvirus-3 from sick calves]. | three herpes strains identified as herpes virus-3 were isolated in revolving test-tubes with cell cultures of nasal samples and lung parenchyma from diseased calves at the time of a respiratory enzooty. large crystalloid aggregates of herpes virus virions were observed in the nucleus and in the cytoplasma by the electron-microscopic investigation performed on infected cell cultures. the experimental infection of calves with the isolated strain produced slight symptoms of respiratory disease in s ... | 1979 | 232333 |
| antibody response in cattle and rabbits to early antigens of malignant catarrhal fever virus in cultured cells. | cytosine arabinoside (ara-c) inhibited the production of late antigens and of infectious virus in monolayers of bovine kidney cells infected with the high-passage, wc-11 strain of malignant catarrhal fever virus. early antigens were not affected. using hyperimmune and acute-phase sera from cattle and rabbits in indirect immunofluorescence tests, it was shown that ara-c treated cultures contained two early antigens; one was diffuse and distributed throughout the cells, the other was particulate a ... | 1978 | 364579 |
| isolation of malignant catarrhal fever virus from a european bison (bos bonasus) in a zoological garden. | 1979 | 400522 | |
| a microtitre technique for the assay of malignant catarrhal fever virus and neutralising antibody. | a microtitre technique for the quantal assay of a cell-free strain of malignant catarrhal fever virus was developed, using serially passaged bovine embryonic kidney cells. end-points were determined after 12 days' incubation and the mean titre recorded for a single virus stock stored at -70 degrees c over a six-month period was 10(5.5) +/- 0.2 (sd). in neutralisation tests serum/virus mixtures were best held at 37 degrees c for 1 h in microtitre trays before the addition of cells; assays were hi ... | 1979 | 523811 |
| observations on two strains of bovine malignant catarrhal fever virus in tissue culture. | two cell-free strains of bovine malignant catarrhal fever (mcf) virus were examined by fluorescent antibody staining and for cytopathogenicity in secondary bovine thyroid (bth) and secondary bovine kidney cell cultures, and in a bovine embryo lung cell line. the hartebeest-derived strain (k30) induced syncytia and intra-nuclear inclusions in all three systems, whereas the widebeest-derived strain (wc11) induced intra-nuclear inclusions in all systems, but syncytia in only bth cells. fluorescent ... | 1976 | 951518 |
| neutralising antibody to herpesviruses dervied from wildebeest and hartebeest in wild animals in east africa. | the sera of 728 game animals, collected in east africa, were tested for neutralising antibody to a strain (wc11) of wildebeest herpesvirus, which is an important cause of malignant catarrhal fever of cattle. in addition, 290 of these sera were tested for neutralising activity against a closely related herpesvirus (k/30) from hartebeest (alcelaphus sp.). antibody was frequently present in three species of the subfamily alcelaphinae (wildebest, hartebeest and topi) and one of the subfamily hippotr ... | 1975 | 1144919 |
| the detection of alcelaphine herpesvirus-1 dna by in situ hybridization of tissues from rabbits affected with malignant catarrhal fever. | tissue sections and cultured lymphocytes from rabbits clinically affected following experimental infection with alcelaphine herpesvirus-1 (ahv-1) were assessed for the presence of viral dna by in situ hybridization with the cloned major hindii repeat sequence of this virus. small numbers of virus-infected cells were consistently detected only in submandibular lymph nodes, while other tissues showed no evidence of viral dna. virus titration in culture suggested that there were higher titres of vi ... | 1992 | 1322946 |
| studies concerning etiology of sheep-associated malignant catarrhal fever in europe. | the etiological agent of sheep-associated malignant catarrhal fever (mcf) in europe has not been isolated directly from sheep. the occurrence of antibodies against the african bovine herpesvirus (bhv-3, wc 11) in cattle and sheep was examined using recently modified indirect immunofluorescence test (iift) and neutralization test (nt) methods. studies revealed that sheep and cattle sera in europe were free from neutralizing antibodies of bhv-3. the iift could not establish the presence of antibod ... | 1992 | 1396168 |
| attempts to immunize cattle against virulent african malignant catarrhal fever virus (alcelaphine herpesvirus-1) with a herpesvirus isolated from american cattle. | two consecutive weekly inoculations with a herpesvirus isolated from sick cattle in america (707k), protected four out of four steers against a first challenge with virulent african malignant catarrhal fever virus (alcelaphine herpesvirus-1), strain c500. three of these steers were still protected in a rechallenge carried out 9.5 months after the first challenge. one inoculation with this agent did not protect such steers, and repeated weekly inoculations had the risk of inducing a malignant cat ... | 1991 | 1651581 |
| failure of sheep to respond to repeated inoculations with an alcelaphine herpesvirus-1-like virus, isolated from a case of malignant catarrhal fever in american cattle. | the replication of an alcelaphine herpesvirus-1-like virus (707k), isolated from a clinical case of malignant catarrhal fever in american cattle, was studied in sheep. viraemia was not observed in any of the six sheep repeatedly inoculated with the 707k virus or in four steers susceptible to malignant catarrhal fever which were housed together with these sheep for one year. none of the four steers seroconverted and only two of the six inoculated sheep showed a negligible and short-lived seroconv ... | 1991 | 1759339 |
| the derivation of a restriction endonuclease map for alcelaphine herpesvirus 1 dna. | the gammaherpesvirus alcelaphine herpesvirus 1 (ahv-1), indigenous to the wildebeest (connochaetes species), is a causative agent of the fatal lymphoproliferative disease malignant catarrhal fever in cattle and deer. the genome of the attenuated wc11 isolate of ahv-1 has previously been shown to possess a region of unique dna together with multiple direct repeat sequences. approximately 70% of the genome of wc11 has now been cloned into plasmid or bacteriophage vectors and these clones have been ... | 1991 | 1850229 |
| recovery of a herpesvirus from a roan antelope (hippotragus equinus). | a herpesvirus was recovered in culture from the cells of a roan antelope (hippotragus equinus) following cryopreservation in dmso and it is thought that the dmso may have been involved in reactivation. the virus was shown to be antigenically related to alcelaphine herpesvirus-1 (ahv-1) of wildebeest and ovine herpesvirus-2 (ohv-2) of domestic sheep (formerly designated the sheep-associated agent of malignant catarrhal fever (mcf]. cloned dna fragments of ahv-1 and ohv-2 cross hybridised with dna ... | 1991 | 1897131 |
| molecular diagnosis of alcelaphine herpesvirus (malignant catarrhal fever) infections by nested amplification of viral dna in bovine blood buffy coat specimens. | a fragment of alcelaphine herpesvirus-1 (ahv-1; malignant catarrhal fever) dna was subcloned into puc 18 and sequenced. the subclone hybridized strongly to ahv-1 dna, weakly to alcelaphine herpesvirus-2 (ahv-2) dna, and not at all to dna from bovine herpesvirus-1 (bhv-1; infectious bovine rhinotracheitis [ibr] virus), bovine herpesvirus-2 (bhv-2; bovine herpes mamillitis [bhm] virus), and bovine herpesvirus-4 (bhv-4; isolate dn599). a 2-stage (nested) polymerase chain reaction (pcr) diagnostic t ... | 1991 | 1911989 |
| the malignant catarrhal fever complex. | malignant catarrhal fever (mcf) is defined as a clinicopathological syndrome caused by related herpesviruses and acquired from persistently infected wildebeest and sheep. there is convincing epidemiologic and virologic evidence that alcelaphine herpesvirus 1 (ahv1) causes the wildebeest-derived disease (wd-mcf). present knowledge suggests that a herpesvirus related to ahv1 may be associated with some cases of the non-wildebeest-associated disease (nwa-mcf). however, this virus possibly represent ... | 1991 | 1934998 |
| restriction endonuclease analysis of alcelaphine herpesvirus 1 dna and molecular cloning of virus genomic dna for potential diagnostic use. | alcelaphine herpesvirus 1 (ahv-1) genomic dna was analyzed using restriction enzymes having recognition sequences both low in guanine-cytosine content (bamhi, kpni, hindiii) and high in guanine-cytosine content (smai, avai, apai). the results from the restriction enzyme analyses along with exonuclease treatment demonstrated that the termini of ahv-1 dna are likely composed of polyrepetitive sequences high in guanine-cytosine content similar to those found in herpesvirus saimiri dna. cleaving ahv ... | 1990 | 1965581 |
| derivation of a dna clone corresponding to the viral agent of sheep-associated malignant catarrhal fever. | malignant catarrhal fever is a fatal lymphoproliferative and degenerative disease of ruminants. one causative agent is the gammaherpesvirus alcelaphine herpesvirus 1 (ahv-1), which produces no disease in its natural host, the wildebeest (connochaetes species). epidemiological evidence implicates sheep as the carrier of a similar virus. however, attempts to culture this virus from sheep or from animals affected with sheep-associated malignant catarrhal fever (sa-mcf) have failed. lymphoblastoid c ... | 1991 | 2047591 |
| [the etiology of "malignant catarrhal fever" originating in sheep: serological findings in cattle and sheep with ruminant gamma herpesviruses]. | in the case of the wildebeest-derived form of malignant catarrhal fever (wd-mcf) alcelaphine herpesvirus 1 (alchv1) is well established as the cause. however, the etiology of the form of the disease circumstantially associated with sheep (sa-mcf) remains equivocal. a serological relationship has been proposed to exist between the unidentified sheep-associated agent causing sa-mcf and alchv1 causing wd-mcf. we attempted to confirm this hypothesis. using an indirect elisa we found 94 of 100 cattle ... | 1991 | 2068708 |
| the biology of bovine herpesvirus-4 infection of cattle. | the biology of bovine herpesvirus-4 (bhv-4) infection of cattle is reviewed. the infection is distributed worldwide. most of isolated viruses are non-pathogenic in cattle; some of them are able to produce a genital disease. twenty-nine structural polypeptides were described; ten of them are glycosylated. two major glycoproteins were characterized by monoclonal antibodies. restriction maps of bhv-4 dna are available for the enzymes ecori, bamhi and hindiii. the strain variations studied by restri ... | 1990 | 2155770 |
| nucleotide sequence of a 3.5 kilobase fragment of malignant catarrhal fever virus strain wc11. | a 3.5 kilobase dna fragment of the malignant catarrhal fever virus (mcfv), strain wc11, was mapped with a number of restriction enzymes, subcloned and sequenced. the fragment was subcloned into plasmid vector, puc19, for direct sequencing. a complete open reading frame of 2,058 base pairs and a partial open reading frame of 630 base pairs were identified. the sequence of 3,389 nucleotides was compared to other herpesviruses. a 310 base pairs sequence in gene a was 57% homologous to a sequence in ... | 1990 | 2167059 |
| [the etiology and epidemiology of malignant catarrh--a review]. | it is generally accepted that both, the wildebeest-derived malignant catarrhal fever (wd-mcf), and the circumstantially evidenced sheep-associated form of the disease (sa-mcf), may be explained as autoimmune disease of various ruminants, namely cattle and farmed deer. the disease follows infection with related herpesviruses being shed by the respective healthy carrier animals. this has convincingly be shown to apply for wd-mcf (alcelaphine herpesvirus 1, alchv1). sa-mcf, however, remains to be c ... | 1990 | 2193384 |
| a diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction. | a sensitive diagnostic method specific for alcelaphine herpesvirus-1, causative agent of malignant catarrhal fever, has been developed. based on the nucleotide sequence of the alcelaphine herpesvirus-1 genomic dna, a pair of 30 nucleotide primers was selected and synthesized for detecting the virus genome using the polymerase chain reaction (pcr). the virus genome was detected in crude cell lysate using the amplification reaction. | 1990 | 2241575 |
| lymphoproliferation in captive wild ruminants affected with malignant catarrhal fever: 25 cases (1977-1985). | the severity of lymphoproliferative disease associated with malignant catarrhal fever was extremely variable among 25 animals at the san diego wild animal park. severe lymphoproliferative disease was seen in 3 of 10 formosan sika deer (cervus nippon taiouanus), 3 of 6 indian axis deer (cervus a axis), 3 of 6 barasingha deer (cervus d duvauceli), and 1 of 3 nilgai (boselaphus tragocamelus). two sika deer and 2 barasingha deer had lesions morphologically indistinguishable from lymphosarcoma. our f ... | 1990 | 2329087 |
| epidemiology of wildebeest-derived malignant catarrhal fever in south africa: inability to transfer the disease with an african face fly musca xanthomelas (diptera: muscidae). | under experimental conditions an african face fly (musca xanthomelas) preferred to feed on cattle dung when provided with a choice of 3 different meals namely sucrose, cattle dung and blood. flies starved overnight fed well on the eyes of cattle and rabbits, but were reluctant to feed again within 2 h after being allowed to feed on cell culture medium or on the eyes of wildebeest, and when they did feed, they preferred to feed on the external side of the eyelids and on the coagulated material in ... | 1990 | 2339002 |
| characterization of envelope proteins of alcelaphine herpesvirus 1. | alcelaphine herpesvirus 1 is a gammaherpesvirus which causes malignant catarrhal fever, an acute lymphoproliferative disorder of cattle and other susceptible bovidae, which is almost invariably fatal. a preliminary analysis of proteins induced by the virus indicated that as many as six glycoproteins and one nonglycosylated molecule might be present in the virus envelope. monoclonal antibodies selected for recognition of virion envelope proteins included two that recognized a complex of infected ... | 1990 | 2352327 |
| effect of interferons on the replication of alcelaphine herpesvirus-1 of malignant catarrhal fever. | four isolates of alcelaphine herpesvirus-1 of malignant catarrhal fever (mcf) were tested for their inducibility of and sensitivity to various interferons. viral isolates from an indian gaur (bos gaurus), a greater kudu (tragelaphus strepsiceros) and two wildebeest (connochaetes gnou) calves did not induce measurable interferon (ifn) in bovine fetal kidney cells. however, these low passages of each virus were all highly cell-associated and viral replication was inhibited at these passages by ifn ... | 1988 | 2457716 |
| production and characterization of monoclonal antibodies to bovid herpesvirus-4. | thirty-five hybridoma cell lines secreting monoclonal antibodies (mabs) against bovid herpesvirus-4 (bhv-4) strain v. test were produced. these hybrid cells resulted from the fusion of sp2/0 myeloma cells with splenocytes of balb/c mice previously immunized with purified bhv-4. a modified indirect fluorescent antibody test (ifat) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. the selected mabs were tested by the same ifat against a panel ... | 1989 | 2546320 |
| alcelaphine herpesviruses 1 and 2 sds-page analysis of virion polypeptides, restriction endonuclease analysis of genomic dna and virus replication restriction in different cell types. | herpesviruses have been isolated from white-tailed, white-bearded and blue wildebeest, as well as from jimela topi and cape hartebeest. these animals are members of the sub-family alcelaphinae of the family bovidae. viruses isolated from wildebeest cause malignant catarrhal fever (mcf) in susceptible ruminant species. alcelaphine herpesviruses (ahv) isolated from wildebeest replicate in both fetal aoudad sheep kidney (fak) cells and bovine embryonic lung (bel) cells. however, virus isolates from ... | 1989 | 2549921 |
| prevalence of antibodies to alcelaphine herpesvirus-1 and nucleic acid hybridization analysis of viruses isolated from captive exotic ruminants. | a serologic survey was conducted to determine the prevalence of antibodies to alcelaphine herpesvirus-1 (ahv-1) in captive exotic ruminants within the united states. forty-six percent of the members of the subfamily alcelaphinae (wildebeest, topi, hartebeest) in the family bovidae had virus-neutralizing antibody to ahv-1. other subfamilies of bovidae with high prevalence of virus-neutralizing antibodies to ahv-1 included hippotraginae (oryx and addax) and caprinae (sheep and goats), with prevale ... | 1989 | 2552876 |
| isolation of bovine herpesvirus-3 from african buffaloes (syncerus caffer). | eleven virus isolations were made from the blood of 45 free living healthy african buffaloes by long term cocultivation of their leucocytes with bovine thymus or spleen cells. the isolates were indistinguishable from each other or from herpesviruses isolated from a severely ill buffalo calf and from a dead buffalo. these viruses possessed the characteristics of the bovine herpesvirus-3 (bhv-3) group and were indistinguishable by serology and restriction endonuclease analysis from the bhv-3 type ... | 1989 | 2662288 |
| antibody to alcelaphine herpesvirus-1 (ahv-1) in hamsters experimentally infected with ahv-1 and the 'sheep-associated' agent of malignant catarrhal fever. | malignant catarrhal fever was induced in four groups of hamsters by the inoculation of cells infected with either the c/500 isolate of alcelaphine herpes-virus-1 (ahv-1) or the sheep-associated agent derived from cattle, red deer or père david's deer. using an indirect immunofluorescence assay, antibody to ahv-1 was detected in sera of clinically affected animals of all four groups. the reaction of sera from hamsters affected with malignant catarrhal fever induced by ahv-1 caused diffuse cytopla ... | 1989 | 2687990 |
| immunoblotting analysis of the reaction of wildebeest, sheep and cattle sera with the structural antigens of alcelaphine herpesvirus-1 (malignant catarrhal fever virus). | malignant catarrhal fever (mcf) is a disease of cattle and some other ruminants caused by alcelaphine herpesvirus-1 (ahv-1), a virus of wildebeest. the disease also occurs in the absence of wildebeest and is then thought to be caused by a viral agent harboured by the sheep. the structural proteins of ahv-1 have been used as antigens for the immunoblotting analysis of sera from wildebeest, sheep and cattle infected by either ahv-1 or the "sheep-associated" form of the disease. wildebeest sera sho ... | 1989 | 2718352 |
| preliminary characterization of the alcelaphine herpesvirus 1 genome. | alcelaphine herpesvirus type 1 (ahv-1) is a causative agent of the fatal lymphoproliferative disease malignant catarrhal fever in deer and cattle. the genomes of the attenuated wc11 isolate and the virulent c500 isolate have been studied. the genome of wc11 comprises a region of unique dna of approximately 130 kbp, which has a g + c content of 50%, and approximately 30 kbp of additional tandem direct repeat sequences with g + c content of 72%. wc11 possesses a major repeat sequence of 950 bp int ... | 1989 | 2732713 |
| excretion of alcelaphine herpesvirus-1 by captive and free-living wildebeest (connochaetes taurinus). | excretion of alcelaphine herpesvirus-1 (ahv-1) is for all practical purposes limited to wildebeest calves under the age of 4 months. sixty-one per cent of calves 1-2 months of age excreted virus with a mean titre of 9.8 x 10(4) cytopathic-forming foci/ml in their ocular fluid. the incidence declined sharply to less than 2% in wildebeest older than 6 months. no difference in age-related excretion of virus could be detected between free-living and captive wildebeest and no virus could be isolated ... | 1989 | 2748133 |
| epizootology of wildebeest-derived malignant catarrhal fever in an outbreak in the north-western transvaal: indications of an intermediate host. | the investigation involved 37 herds of cattle numbering 6,280 animals and 5 groups of blue wildebeest (connochaetes taurinus), consisting of 30-330 wildebeest per group. all the cases of wildebeest-derived malignant catarrhal fever encountered were associated with wildebeest and not with other game animals. six per cent of the cases were encountered in late summer when the wildebeest calves were 3-4 months old, whereas 73% occurred in spring, when the wildebeest calves were 8-11 months old and d ... | 1989 | 2748134 |
| immunological relationships between malignant catarrhal fever virus (alcelaphine herpesvirus 1) and bovine cytomegalovirus (bovine herpesvirus 3). | strains of malignant catarrhal fever virus (alcelaphine herpesvirus 1 (ahv-1)) and bovine cytomegalovirus (bovine herpesvirus 3 (bhv-3)) were compared for serological relatedness by cross-titration in an indirect immunofluorescent (iif) antibody assay. there was definite cross-reactivity between these 2 viruses, with heterologous sera staining intracellular and membrane antigens of infected cells. heterologous antibody titres were approximately 50-fold lower than homologous titres and could be r ... | 1988 | 2836993 |
| cloning and characterization of a genomic probe for malignant catarrhal fever virus. | a genomic probe specific for malignant catarrhal fever (mcf) virus was cloned by using purified viral dna from mcf-virus strain wcll. restriction endonuclease analysis of the purified viral dna was used to identify the cloned viral genomic fragment. dot blot hybridization by use of the genomic probe (prp-5) indicated that the probe hybridized specifically with wcll-mcf virus, as well as with one other isolate of mcf-associated herpesvirus. hybridization also was observed to a non-mcf virus strai ... | 1988 | 2847602 |
| an unusual clinical and pathological variant of malignant catarrhal fever in a white-tailed deer. | a captive male white-tailed deer (odocoileus virginianus) developed an acute illness over a 3-day period characterized predominantly by neurological, ocular and respiratory signs which were accompanied by prominent gross lesions of multiple organ systems. histologically, a proliferative vasculitis consisting primarily of lymphocytic-lymphoblastic cellular infiltration was found in ocular, oral, respiratory, cardiac and neural tissues. the extensive nature of these infiltrations resulted in gross ... | 1989 | 2915391 |
| concurrent malignant catarrhal fever and bovine virus diarrhoea virus infection in a dairy herd. | an account is given of an outbreak of malignant catarrhal fever which occurred in a 98-cow dairy herd. ten animals died or were slaughtered and the disease was confirmed by clinical and histological examination. serological tests for malignant catarrhal fever virus were positive in three of four animals. the diagnosis of malignant catarrhal fever was complicated by the presence of bovine virus diarrhoea virus infection in three of the early cases. the initial cases of malignant catarrhal fever o ... | 1987 | 3039713 |
| lytic function of bovine lymphokine-activated killer cells from a normal and a malignant catarrhal fever virus-infected animal. | lymphokine-supplemented long-term cultured bovine lymph node lymphocytes were characterized functionally and phenotypically. lymphocytes from a normal and a malignant catarrhal fever (mcf) virus-infected animal were maintained without the addition of antigen or feeder cells. lymphocyte cell lines obtained from both animals: (i) killed allogeneic fibroblasts and allogeneic and xenogeneic cultured tumor cell lines as measured in a 4-h 51cr release assay, (ii) expressed the same t cell subset marke ... | 1988 | 3263729 |
| transmission of wildebeest-associated and sheep-associated malignant catarrhal fever to hamsters, rats and guinea-pigs. | wildebeest-derived malignant catarrhal fever (wd-mcf) was transmitted to hamsters, rats and guinea-pigs by inoculation of rabbit lymphoid cells infected with alcelaphine herpesvirus-1, strain c-500. sheep-associated mcf (sa-mcf) was transmitted to hamsters by inoculation of lymphoid cells from rabbits affected with sa-mcf derived from deer. mice were refractory to both forms of the disease. with both forms of mcf, the incubation period during initial transmission varied from 21 to 90 days and di ... | 1988 | 3346393 |
| the pathology of wildebeest-associated malignant catarrhal fever in hamsters, rats and guinea-pigs. | lesions typical of malignant catarrhal fever were found in hamsters, rats and guinea-pigs inoculated with a rabbit-passaged strain (c-500) of alcelaphine herpesvirus-1. lesions found during primary passage included proliferation of lymphoid tissues, multisystemic mononuclear cell infiltrates, vasculitis and necrosis, especially in the alimentary tract. the character, severity and distribution of lesions remained stable in affected hamsters during serial passage of disease, whereas lympho-prolife ... | 1988 | 3346394 |
| enzyme-linked immunosorbent assay for the detection of antibodies to the alcelaphine herpesvirus of malignant catarrhal fever in exotic ruminants. | an elisa for antibodies to the alcelaphine herpesvirus-1 of malignant catarrhal fever was developed. of sera that represented 42 exotic ruminant species, 216 were evaluated by the elisa and a virus-neutralization test. a significant correlation (r = 0.564, p less than 0.001, n = 216) between the elisa and virus-neutralization test results was found. of the sera having positive test results by virus neutralization, 86.1% also had positive results by the elisa, and of the sera having negative test ... | 1988 | 3348526 |
| use of vero cells for the isolation and propagation of malignant catarrhal fever virus. | vero cells were compared with primary bovine thyroid (bth) cultures for the isolation of malignant catarrhal fever virus from infected blood and tissues. comparative titrations showed vero cells detected only two-fold less infectivity in rabbit spleen suspensions than bth cells. twenty three of 32 bovine buffy coat cell preparations which were positive on bth cells were also positive on vero cells. the cytopathic effects (cpe) of virus isolates in vero cells consisted of syncytia and refractile ... | 1987 | 3629721 |
| dexamethasone-induced recrudescence of malignant catarrhal fever and associated lymphosarcoma and granulomatous disease in a formosan sika deer (cervus nippon taiouanus). | malignant catarrhal fever (mcf) was diagnosed in a 2-week-old formosan sika deer. the fawn had been previously exposed to a clinically normal neonatal wildebeest calf from which alcelaphine herpesvirus-1 was isolated. alcelaphine herpesvirus-1 was isolated from buffy coat leukocytes and nasal and ocular secretions of the fawn during the acute illness. the fawn clinically recovered after 3 weeks. virus was not recovered from blood at this time. dexamethasone, given 4 months after clinical recover ... | 1985 | 3839641 |
| ultrastructure of cellular changes in the replication of the alcelaphine herpesvirus-1 of malignant catarrhal fever. | cell cultures inoculated with 5 different viral isolates from 4 species of ruminants with clinical signs of malignant catarrhal fever (from the san diego wild animal park) were examined by electron microscopy. each had the morphology of a herpesvirus (118 to 220 nm) and was icosahedral, and the nucleocapsid matured in the nucleus of the infected cell. envelopment of budding occurred with each viral isolate at the nuclear and the plasma membranes. the virions egressed from the cell by budding fro ... | 1985 | 4040722 |
| malignant catarrhal fever virus in nasal secretions of wildebeest: a probable mechanism for virus transmission. | 1974 | 4436931 | |
| a preliminary report on the isolation of bovine malignant catarrhal fever virus in iran. | 1972 | 4651425 | |
| the attenuation of a herpes virus (malignant catarrhal fever virus) isolated from hartebeest (alcelaphus buselaphus cokei gunther). | 1973 | 4778485 | |
| neutralising antibodies to wildebeest-derived malignant catarrhal fever virus in african wildlife. | a total of 2,722 sera collected between 1963 and 1983, from 43 different species of wildlife in 11 african countries was examined for neutralising antibodies against the wildebeest-derived strain of malignant catarrhal fever (mcf) virus. antibodies were demonstrated in 10 species of bovidae which included eight species from the sub-family hippotraginae and one species each from bovinae and antilopinae. neutralising antibodies were also recorded in hippopotamus. it is suggested that the high prev ... | 1984 | 6099787 |
| purification of african malignant catarrhal fever virus using a two-phase aqueous polymer system. | the wc11 isolate of malignant catarrhal fever virus (mcfv) was purified by a method employing phase separation of the virus in an aqueous polymer system. virus was grown in primary bovine thyroid cells. the virus released into the extracellular medium was purified. initial concentration and partial purification of mcfv occurred after separation of the virus into the dextran phase following the addition of 10% (w/v) polyethylene glycol and 8% (w/v) dextran t10 to the extracellular virus. further ... | 1982 | 6175727 |
| cross-reactions of bovine herpesvirus 1 antigens with those of other cattle herpesviruses. | bovine embryonic kidney cells were infected with bovine herpesviruses (bhv1, 2, or 3), suid herpesvirus 1 (shv1), or were sham-inoculated. when cytopathic effect was apparent, the cells were solubilized using triton x-100 detergent. resulting antigen preparations were tested by 2-dimensional immunoelectrophoresis using bovine fetal serum and antisera directed against bhv1, bhv2, bhv3, shv1 or a restricted spectrum of bhv1 antigens. interaction of bhv1 antiserum with bhv1 antigen preparations res ... | 1984 | 6208673 |
| effect of levamisole on the course of malignant catarrhal fever virus infection in rabbits. | 1981 | 6262959 | |
| further studies of naturally occurring latent bovine herpesvirus infection. | attempts were made to enhance the isolation rate of latent bovine herpesviruses from trigeminal ganglia by using a fibroblastic fetal bovine kidney cell line, in addition to madin-darby bovine kidney cells, and by superinfecting with a temperature-sensitive helper strain of bovine herpesvirus-1 (bhv-1). four isolates of latent bhv-1 were obtained from 44 pairs of trigeminal ganglia--thus reflecting no increase in isolation rate over that previously observed. two isolates of a latent bovine herpe ... | 1981 | 6275750 |
| bovine cytomegaloviruses: identification and differential properties. | biological properties and restriction enzyme patterns of the slowly replicating herpesviruses isolated from cattle affected with different diseases in north america and europe were analysed. these virus isolates induced identical plaques that developed within 7 to 9 days in bovine foetal spleen cells and within 5 days in actively growing georgia bovine kidney cells. these virus isolates were found to be antigenically related when tested in the indirect immunofluorescence test, and antigenic rela ... | 1984 | 6323619 |
| antibodies to malignant catarrhal fever virus in cattle with non-wildebeest-associated malignant catarrhal fever. | sera from 48 cattle with non-wildebeest-associated malignant catarrhal fever (mcf) were assayed for antibodies to wildebeest-associated mcf virus. significant titres were found in 38 sera and these reactions appeared to be specific. some cases of non-wildebeest-associated mcf may be caused by an agent antigenically related to mcfv. | 1983 | 6341420 |
| antibodies in carrier wildebeest to the lymphoproliferative herpesvirus of malignant catarrhal fever. | six types of antibody to malignant catarrhal fever virus (mcfv) were measured in 132 sera collected from wildebeest in kenya masailand. the titre of all types of antibody declined slowly with increasing age of the wildebeest. a significantly greater proportion of wildebeest calves had higher titres of antibodies to mcfv early antigens, igm antibodies to mcfv late antigens and complement-fixing antibodies, than did older animals. one seronegative calf, reared in isolation without colostrum, becam ... | 1983 | 6402335 |
| course of malignant catarrhal fever in immunosuppressed and immunostimulated rabbits. | rabbits pretreated with cyclophosphamide for 7 days (cy+) and control rabbits (cy-), were infected with cell-associated malignant catarrhal fever virus (mcfv), and cy treatment of the cy+ group was continued to day 20 p.i. the time of onset and degree of antibody formation to bovine serum albumin was significantly suppressed in the cy+ group whilst the humoral antibody response to mcfv appeared to be delayed rather than suppressed. although incubation periods of the disease were comparable in th ... | 1984 | 6539032 |
| proliferation of t lymphoblasts in rabbits fatally infected with the herpes virus of malignant catarrhal fever. | lymphoid cell suspensions prepared from tissues collected from rabbits infected with malignant catarrhal fever virus, a herpes virus causing fatal lymphoproliferative disease in cattle and rabbits, were examined for various properties. the majority of the proliferating cells did not adhere to plastic, phagocytose opsonized bacteria or carry surface immunoglobulin. on the basis of their morphology, high incorporation of 3h-thymidine and ability to form non-immune rosettes with rabbit erythrocytes ... | 1983 | 6606511 |
| role of wildebeest fetal membranes and fluids in the transmission of malignant catarrhal fever virus. | malignant catarrhal fever virus was not isolated from samples of fetal membranes or fluid collected from 93 calving wildebeest (connochaetes taurinus) in kenya maasailand. cell-free strains of malignant catarrhal fever virus were very rapidly inactivated when exposed to the sun under field conditions, at least 3.0 log10 units/25 microliter being lost per hour at midday. it is suggested that wildebeest fetal membranes and fluids act as visual markers for areas of pasture which are particularly he ... | 1983 | 6623872 |
| attempts to protect rabbits against challenge with virulent, cell-associated, malignant catarrhal fever virus. | rabbits hyperimmunized with inactivated malignant catarrhal fever virus (mcfv) infected rabbit lymph node cells did not develop specific antibodies to the virus and succumbed to challenge with live mcfv-infected lymphoid cells. rabbits hyperimmunized with either inactivated or live, cultured bovine kidney cells infected with mcfv developed antibodies to the virus, but also succumbed to challenge with live mcfv-infected rabbit lymphoid cells. rabbits hyperimmunized with live cultured rabbit kidne ... | 1982 | 6761953 |
| antibodies to malignant catarrhal fever virus antigens in the sera of normal and naturally infected cattle in kenya. | six different serological tests were used to examine kenyan cattle sera for antibodies to the herpesvirus of malignant catarrhal fever. significantly higher levels of indirect immunofluorescent antibody to early and late virus antigens and of complement fixing antibody were found in the sera of 13 naturally infected cattle than in 482 sera collected from four different groups of normal cattle. virus neutralising and immunoprecipitating antibodies were also found in some infected cattle sera but ... | 1980 | 6781021 |
| a lack of readily demonstrable virus antigens in the tissues of rabbits and cattle infected with malignant catarrhal fever virus. | 1980 | 6784880 | |
| infectivity of cell-free malignant catarrhal fever virus in rabbits and cattle. | 1983 | 6868354 | |
| effect of malignant catarrhal fever virus infection on the immune response of rabbits to sheep red blood cells. | rabbits infected with african malignant catarrhal fever virus mounted a depressed antibody response to sheep red blood cells compared to the antibody response of uninfected rabbits. immunodepression was observed in rabbits immunised 0, 3, 5, 7 and 10 days after infection. antibody response was not depressed in the rabbits immunised 1 day after infection. despite the depressed antibody response to srbc, the rabbits died with rising virus neutralising antibody to the virus. the possible causes of ... | 1982 | 6890887 |
| malignant catarrhal fever virus specific secretory iga in nasal secretions of wildebeest calves. | wildebeest iga was isolated from nasal secretions and precolostrum. it was identified by cross-reaction with anti-human and anti-bovine iga sera. nasal secretions collected from wildebeest calves over 3 months old had malignant catarrhal fever virus neutralizing antibody activity. they also contained specific iga to the virus as detected by indirect immunofluorescence. it is suggested that production of malignant catarrhal fever virus specific iga in the nasal cavity, contributes to the eliminat ... | 1982 | 6890888 |
| target cells for malignant catarrhal fever virus in rabbits. | lymphocytes from lymph nodes and spleens of malignant catarrhal fever virus (mcfv) infected rabbits were tested for mcfv infectivity in secondary calf thyroid cell monolayers. most infectivity was demonstrated in the lymphocytes. some infectivity was also detected in macrophages/monocytes. it was thus concluded that lymphocytes form the major target cell for the herpesvirus of malignant catarrhal fever in rabbits. | 1982 | 6981238 |
| immunoglobulin response of rabbits infected with malignant catarrhal fever virus. | 1982 | 6982493 | |
| the protection of rabbits against the herpesvirus of malignant catarrhal fever by inactivated vaccines. | cell-free virulent malignant catarrhal fever virus inactivated by either formalin or acetylethylenimine (aei) and administered with freund's complete adjuvant protected rabbits against two consecutive parenteral challenges with cell-free virus. however 'protected' rabbits succumbed within 20 days to later inoculation of infected rabbit lymph node suspensions. both inactivated vaccines evoked high titres of neutralising and immunofluorescent antibody, but more of the rabbits given the aei prepara ... | 1980 | 6997950 |
| immunofluorescence and immunoperoxidase techniques for detecting antibodies to malignant catarrhal fever in infected cattle. | the indirect immunoperoxidase and immunofluorescent antibody techniques were compared for their ability to detect antibodies to malignant catarrhal fever virus in experimentally and naturally infected cattle. the immunoperoxidase test detected titres of antibody 8-fold higher than those detected by immunofluorescence. the immunoperoxidase technique detected antibodies in all of 23 naturally infected cattle whereas immunofluorescence only detected antibodies in 19. the immunoperoxidase test gave ... | 1981 | 7046177 |
| antibodies to malignant catarrhal fever virus in sheep sera. | 1981 | 7047584 | |
| epidemiology of bovine malignant catarrhal fevers, a review. | the mode of transmission of malignant catarrhal fever virus (mcfv) from wildebeest to cattle has been obscure for some time. recent studies on the virus shedding patterns of wildebeest have revealed that mcfv occur in nasal and ocular secretions of young wildebeest in a stable, cell-free state. such cell-free virus is not found in the secretions of mcfv infected cattle. the findings indicate that mcfv is transmitted from wildebeest to cattle as cell-free virus shed in the secretions of young wil ... | 1981 | 7048724 |
| electron microscopic study of the african strain of malignant catarrhal fever virus in bovine cell cultures. | malignant catarrhal fever (african strain) is a viral disease of ruminants which is considered an exotic disease in the united states. viral isolates obtained from one clinically ill gaur (bos gaurus) and a greater kudu (tragelaphus strepsiceros) located in a zoologic park in oklahoma, and from one heifer (bos taurus) and a domestic white-tailed deer (odocoileus virginianus) experimentally inoculated with the isolated and identified african strain of malignant catarrhal fever virus (mcfv), were ... | 1982 | 7073076 |
| the effect of antibody and complement on the expression of herpesvirus of bovine malignant catarrhal fever in cultured rabbit lymphocytes. | using indirect immunofluorescence with a hyperimmune calf serum, a virus-induced antigen was demonstrated on the surface of lymphocytes expression intracellular malignant catarrhal fever virus antigens. antibody to the antigen was also detected in terminal sera of both cattle and rabbits. antisera did not restrict virus expression in explanted lymph nodes unless they were supplemented with two to four units of lytic complement per ml culture. while human, bovine and guinea pig complements caused ... | 1982 | 7179718 |
| isolation of bovine malignant catarrhal fever virus from ocular and nasal secretions of wildebeest calves. | malignant catarrhal fever (mcf) virus was isolated from ocular secretions of four out of nine free-ranging wildebeest calves, while four out of 11 animals had the virus in nasal secretions. the mean infectivity of virus in ocular and nasal secretions was 10(2.25)tcid50/ml and 10(2.5)tcid50/ml respectively but infectivity of more than 10(3.2)tcid50/ml was found in some ocular and nasal secretions. thus it appears that mcf virus is excreted via the ocular and nasal routes from wildebeest calves, a ... | 1980 | 7193339 |
| prevalence of virus neutralising antibodies to malignant catarrhal fever virus in oryx (oryx beisa callotis). | 1981 | 7198155 | |
| malignant catarrhal fever virus infectivity in rabbit macrophages and monocytes. | malignant catarrhal fever virus (mcfv) infectivity was demonstrated in macrophages/monocytes collected from mcfv-infected rabbits. thus, in addition to lymphocytes, macrophages and monocytes are probably involved in the pathogenesis of mcfv and could account for the widespread dissemination of mcfv, the very high mortality rate and the immunosuppression observed in mcfv-infected animals. | 1981 | 7201203 |
| malignant catarrhal fever. i. response of american cattle to malignant catarrhal virus isolated in kenya. | fifty-three american cattle were inoculated with malignant catarrhal fever virus isolated from a wildebeest in kenya. three animals showed the mild form of the disease and recovered, and 47 showed the severe form of the disease. the other three did not react. of the 47 cattle, 28 died, 16 were killed for the collection of specimens and three recovered. the incubation period for the 47 cattle ranged from 16 to 29 days and the course of the fatal disease for 28 cattle averaged three to 23 days. vi ... | 1981 | 7272843 |
| malignant catarrhal fever virus shedding by infected cattle. | 1981 | 7296019 | |
| influence of temperature on the growth in cell culture of malignant catarrhal fever virus. | the growth characteristics of malignant catarrhal fever (mcf) virus in bovine thyroid cultures were affected by incubation temperature. the cytopathic effect at 37 degrees c was predominantly syncytial and little or no cell-free virus could be detected. at 32 degrees to 34 degrees c foci of rounded refractile cells were observed, and this was accompanied by an increase in the amount of cell-free virus found in culture fluids. growth curve studies with one low passage isolate of mcf virus showed ... | 1981 | 7323461 |
| comparative pathology of the african form of malignant catarrhal fever in captive indian gaur and domestic cattle. | the african strain of malignant catarrhal fever virus was isolated and identified in a herd of captive indian gaur (bos gaurus). gross lesions included oral ulcerations, corneal opacity, and multifocal myocarditis. microscopic lesions were characterized by lymphocytic vasculitis/perivasculitis in all tissues examined. blood was serially passed from a gaur to a domestic heifer (bos taurus), and then to a domestic calf. clinical signs and gross and microscopic lesions similar to those of the gaur ... | 1981 | 7327993 |
| malignant catarrhal fever iii. experimental infection of sheep, domestic rabbits and laboratory animals with malignant catarrhal fever virus. | five of 19 sheep became infected when inoculated with a virulent strain of malignant catarrhal fever virus isolated in kenya one of the infected animals was killed in extremis; its blood and lymph node suspension reproduced the classical disease in three steers. calves exposed to these sheep did not become infected during 89 days of close contact. the kenya strain of malignant catarrhal fever virus infected rabbits, guinea pigs and hamsters, producing occular and nasal discharges, paralysis and ... | 1981 | 7340914 |
| a complement fixation test for antigens of and antibodies to malignant catarrhal fever virus. | a complement fixation test was developed for the study of malignant catarrhal fever (mcf). a clarified ultrasonicate of cultured cells infected with mcf virus was used as antigen and detected increasing levels of complement fixing antibody in 12 out of 14 and four out of nine infected cattle and rabbits respectively. titres greater than or equal 1:64 were found in hyperimmune sera from three out of four cattle, five out of five rabbits and in eight out of 143 wildebeest. no antibody was detected ... | 1980 | 7414069 |
| a plaque assay for malignant catarrhal fever virus and virus neutralizing activity. | a cell-free strain of malignant catarrhal fever virus which produced a readily recognizable cytopathic effect was obtained by serial passage of the virus in a rabbit kidney cell line. plaque assay of the virus was more rapid and gave higher titres 11 days postinoculation than tube titration, but the latter advantage decreased with a longer incubation period. plaques were clear with sharp edges and measured 0.5 to 2 mm in diameter after 15 days. a plaque neutralization test was developed and succ ... | 1980 | 7427840 |
| the proliferation of malignant catarrhal fever virus in cattle and rabbits. | 1980 | 7437548 | |
| competitive inhibition enzyme-linked immunosorbent assay for antibody in sheep and other ruminants to a conserved epitope of malignant catarrhal fever virus. | malignant catarrhal fever (mcf) is a severe, usually fatal, acute systemic disease syndrome of certain domestic and wild ruminants caused by members of the family gammaherpesvirinae. two distinct but closely related viruses cause clinically indistinguishable syndromes: one that is indigenous to the widebeest and the other that apparently is indigenous to domestic sheep. neither the pathogenesis nor the epidemiology of sheep-associated mcf (sa-mcf) is understood, primarily because of a lack of ad ... | 1994 | 7523438 |
| investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by pcr and competitive inhibition enzyme-linked immunosorbent assay. | development of control measures for the gammaherpesviral disease of cattle known as sheep-associated malignant catarrhal fever (sa-mcf) has been hampered by a lack of accurate diagnostic tests either for the causative virus or for antibody against that virus. a recently developed competitive-inhibition enzyme-linked immunosorbent assay (ci-elisa) for the detection of antibody to malignant catarrhal fever (mcf) virus (mcfv) in ruminants based on a monoclonal antibody to a widely conserved epitope ... | 1995 | 7559946 |
| identification and analysis of an alcelaphine herpesvirus 1 (ahv-1) cdna clone expressing a fusion protein recognized by ahv-1-neutralizing antisera. | rabbit antiserum to psoralen-inactivated alcelaphine herpesvirus 1 (ahv-1) virions was shown to react specifically with ahv-1-infected cells by indirect immunofluorescence. western blot analysis using this antiserum identified a 15-kd virion protein that was also detected in infected-cell proteins between 12 and 144 h p.i., and a 37-kd protein present in infected cells between 24 and 120 h p.i. a cdna library was constructed using mrna obtained from ahv-1-infected fetal mouflon sheep kidney (fms ... | 1995 | 7733826 |
| multiple superinfections fail to activate defective human immunodeficiency virus-1 (hiv-1) infection of rabbits. | superinfection of human immunodeficiency virus (hiv)-1-infected rabbits with treponema pallidum, mycobacterium avium, herpes simplex, candida albicans, mycoplama incognitus, and malignant catarrhal fever virus, as well as irradiation or cortisone treatment, fails to activate production of infectious virus. for up to 6 months after infection of rabbits with hiv-infected cells or free virus, there are neither clinical symptoms nor positive laboratory tests for detection of hiv. however, after supe ... | 1995 | 7788421 |
| [development of pcr tests for the detection of bovine herpesvirus-1, bovine respiratory syncytial viruses and pestiviruses]. | the development of pcr assays for detection of bhv-1, brsv, bvdv and another pestiviruses is summarized. a polymerase chain reaction assay based on primers selected from the viral gi glycoprotein gene detected 3 fg pure bhv-1 dna, 0.1-1.0 tcid50 or a single infected cell. no amplification was observed with dna from bhv-2, bhv-3, bhv-4, ohv-1 or ohv-2. however, a fragment of the correct size (468 bp) was amplified using dna from herpesviruses isolated from reindeer, red deer and goat. the pcr ass ... | 1994 | 7817501 |
| pcr detection of ovine herpesvirus-2 dna in indonesian ruminants--normal sheep and clinical cases of malignant catarrhal fever. | malignant catarrhal fever (mcf), a fatal viral disease of cattle and other large ruminants, has a worldwide distribution. there are two forms of the disease, one of which, is caused by alcelaphine herpesvirus-1 (ahv-1) and is derived from wildebeest. the other form is associated with domestic sheep and is caused by ovine herpesvirus-2 (ohv-2). the disease in indonesia is sheep-associated with the preferred livestock of this area, balinese cattle (bos javanicus) and water buffalo (bubalus bubalis ... | 1994 | 7839584 |
| rapid detection of bovine herpesvirus 1 (bhv 1) using the polymerase chain reaction. | a polymerase chain reaction (pcr) assay based on primers from the viral gi glycoprotein gene detected 3 fg pure bhv-1 dna, 0.1-1.0 tcid50 or a single infected cell. no amplification was observed with dna from bhv-2, bhv-3, bhv-4, ohv-1 or ohv-2. however, a fragment of the correct size was amplified using dna from herpesviruses isolated from reindeer, red deer and goats. the pcr assay was able to detect virus in nasal swabs up to 14 days after experimental infection of cattle and there was a good ... | 1994 | 7839585 |
| identification and characterization of the major proteins of malignant catarrhal fever virus. | malignant catarrhal fever virus (mcfv), a gamma-herpesvirus, causes a severe inflammatory and lymphoproliferative disease of cattle and other susceptible ruminants. polyclonal antisera and monoclonal antibodies (mabs) to the minnesota isolate of mcfv were produced and used to examine the characteristics of the viral proteins. immunoprecipitation of antigens of the minnesota isolate of mcfv with polyclonal antisera revealed at least 11 proteins with molecular masses ranging from 17 kda to 145 kda ... | 1995 | 7844521 |
| application of polymerase chain reaction to detect animals latently infected with agents of malignant catarrhal fever. | oligonucleotide primers derived from alcelaphine herpesvirus 1 (ahv-1) isolate wc11 dna, the first identified agent of malignant catarrhal fever (mcf), were used to assay blood lymphocyte dna using the polymerase chain reaction (pcr). multiple species of exotic ruminants were examined to determine the suitability of this technique for detecting animals that may be latently infected. to correlate the pcr results with those of serology, serum samples were obtained from each animal concurrently wit ... | 1994 | 7858018 |
| diagnosis of malignant catarrhal fever by polymerase chain reaction amplification of alcelaphine herpesvirus 1 sequence. | we derived sequence information from cloned hindiii fragment "d" of alcelaphine herpesvirus 1 strain wc11, an agent of malignant catarrhal fever (mcf). based on this sequence, oligonucleotide primers were selected and synthesized for use in a polymerase chain reaction amplification assay. these primers were used to test samples of total nucleic acids isolated from multiple tissues taken from an indian gaur (bos guarus gaurus) at the san diego wild animal park in san diego, california (usa) which ... | 1994 | 7933281 |
| polymerase chain reaction amplification of wildebeest-associated and cervine-derived malignant catarrhal fever virus dna. | a polymerase chain reaction (pcr) assay was developed for the detection of alcelaphine herpesvirus 1 (ahv1), a causative agent of malignant catarrhal fever (mcf) of ruminants. a pair of 20-base primers was constructed based on the published nucleotide sequence of gene a of the wc11 isolate of ahv1 and was used to amplify a dna fragment of 413 base pairs. the optimised pcr assay was highly sensitive, i.e. it detected 10 fg of genomic dna of ahv1 (wc11 isolate). the amplified fragment was shown to ... | 1994 | 7979973 |
| generation of a nucleic acid probe specific for the alcelaphine herpesvirus 1 and its use for the detection of malignant catarrhal fever virus dna in blue wildebeest calves (connochaetes taurinus). | two wc 11 specific dna fragments, 3 kb and 2 kb in size, respectively, were cloned and evaluated as probes for their use in diagnostic and epidemiological investigations of malignant catarrhal fever (mcf). field specimens including blood, ocular fluid, nasal mucus and urine of blue wildebeest (connochaetes taurinus) calves in the kruger national park, south africa, were tested and found positive for excretion of mcf-virus by slot blot hybridization. in 2 cases mcf-virus dna was detected in the u ... | 1993 | 8332328 |
| pcr detection of the sheep-associated agent of malignant catarrhal fever. | from a genomic library previously constructed from a lymphoblastoid cell line (lcl) propagated from a bovine case of sheep-associated malignant catarrhal fever (sa-mcf), caused by ovine herpesvirus-2 (ohv-2), several ohv-2 clones were identified and characterised by hybridisation using probes from the unique region of the alcelaphine herpesvirus-1 (avh-1) genome. nucleotide sequence from one clone was generated and the predicted amino acid sequence was found to contain regions of homology with t ... | 1993 | 8352654 |
| resistance and susceptibility of bovine cells expressing herpesviral glycoprotein d homologs to herpesviral infections. | bovine cell lines individually expressing two related herpesviral proteins, pseudorabies virus glycoprotein 50 and herpes simplex virus type 1 glycoprotein d, were examined for their susceptibility/resistance to infection with several alphaherpesviruses. cell lines expressing gp50 or gd-1 resisted plaque formation by the homologous virus more than by the heterologous viruses. bovine cells expressing bovine herpesvirus 1 glycoprotein iv (giv) were susceptible to infection with three other bovine ... | 1993 | 8386880 |
| [detection of bovine herpesvirus-1 using the dot-blot hybridization method]. | with the development of molecular biology in the 1980s methods of microorganism detection start to innovate. one of the main advantages of the molecular-genetic methods, namely hybridization of nucleic acids and pcr methods, is the detection of genome of microorganism without the need for cellular cultivation. to detect bhv-1 (etiological agens ibr-ipv) the dot-blot hybridization method on nitrocellulose filters was used together with different types of dna probes (two-fiber recombinant plasmids ... | 1993 | 8390122 |
| development of a dna probe for identification of bovine herpesvirus 4. | a sensitive and specific dna probe for detection and identification of bovine herpesvirus 4 (bhv-4) was developed. cloned fragments from a library of hindiii fragments of the bhv-4 (dn-599) genome were labeled with 32p or digoxigenin and were tested for sensitivity and specificity in detecting viral dna by dot-blot hybridization. two probes were identified that detected 10 pg of purified viral dna, and detected viral dna in 0.001 micrograms of total dna extracted from bhv-4-infected cells. both ... | 1993 | 8391228 |