Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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the regulated catabolism of endogenous and exogenous phosphatidylinositol by saccharomyces cerevisiae leading to extracellular glycerophosphorylinositol and inositol. | it was previously shown that phosphatidylinositol catabolism leads to the accumulation of glycerophosphorylinositol in the culture medium of saccharomyces cerevisiae. we now find that lack of an energy source (glucose) reduces the formation of glycerophosphorylinositol and increases extra-cellular inositol. this situation is reversed by refeeding glucose. [3h]phosphatidylinositol is the precursor of extra-cellular [3h]inositol with energy-starved cells. extracellular glycerophosphorylcholine and ... | 1975 | 166987 |
the distribution of pyrophosphatidic acid in nature. | the occurrence of a novel phospholipid, pyrophosphatidic acid, in the lipid extracts of yeasts (23 species), bacteria (e. coli), algae (chlorella), mammalia (human, rabbit, guinea pig, and mouse), insect (cockroach), fish (carp), mollusc (clam), and spermatophyta (spinach) was investigated. pyrophosphatidic acid was found exclusively in the lipid extracts of several kinds of yeast species, but not in other normal living species (animals, plants, and microorganisms) so far investigated. all of th ... | 1975 | 175047 |
[influence of some thiamine antagonists on the growth of kloeckera apiculata, lactobacillus fermenti and lactobacillus arabinosus (author's transl)]. | 1977 | 557103 | |
[yeast flora of plant rhizosphere and phyllosphere]. | the species belonging to the genera cryptococcus and hansenula with saturnian spores predominate in the rhizosphere of agricultural plants. the sporiferous strains of debaryomyces, hanseniaspora apiculata, metschnikowia pulcherrima, and asporogenic candida krusei and trichosporon cutaneum prevail in the rhizosphere of wild plants. candida krusei and trichosporon cutaneum prevail in the rhizosphere of wild plants. the cultures of rhodotorula, candida krusei and metschnikowia pulcherrima are typic ... | 1975 | 1226148 |
the conversion of 3-hydroxy-2,4,5-trihydroxymethylpyridine into pyridoxine by kloeckera apiculata. | kloeckera apiculata, a vitamin b-6-dependent yeast, grows in the presence of 3-hydroxy-2,4,5-trihydroxymethylpyridine in vitamin b-6-free media. on a molar basis the growth-promoting activity of this compound is approximately one-tenth that of other forms of vitamin b-6. [g-3h]3-hydroxy-2,4,5-trihydroxymethylpyridine is converted into radioactive vitamin b-6 compounds of the same specific radioactivity by growing cultures of k. apiculata. | 1976 | 1275911 |
activity of different 'killer' yeasts on strains of yeast species undesirable in the food industry. | killer strains of the genera saccharomyces, hansenula and kluyveromyces were tested for killing activity against yeasts that cause trouble in the food industry (in the genera zygosaccharomyces, kloeckera, saccharomycodes and schizosaccharomyces). saccharomyces strains killed only zygosaccharomyces rouxii strains, while non-saccharomyces strains showed a wider anti-yeast spectrum. the kluyveromyces phaffii killer strain was of particular interest because of its killer action against kloeckera api ... | 1991 | 1769559 |
contribution of different yeasts isolated from musts of monastrell grapes to the aroma of wine. | volatile substances of wines obtained by fermentation of musts from 'monastrell' grapes (alicante, spain) was studied for yeast isolated from such musts. the results of the statistical treatment performed show the importance of yeasts of low fermentative power, particularly kloeckera apiculata, in the production of volatile substances. saccharomyces cerevisiae var. chevalieri was found to be the most important yeast of high fermentative power. | 1991 | 1777384 |
physiological variants of saccharomyces cerevisiae and kloeckera apiculata from palm wine and cashew juice. | physiological variants of saccharomyces cerevisiae and kloeckera apiculata have been identified in oil palm wine and cashew juice from nigeria. genomic dna from the four s. cerevisiae variants had a % g + c of 36-41% while that of k. apiculata was 32.2%. fermentation of cashew juice produced wine of alcoholic contents of 10% with s. cerevisiae, 8% with k. apiculata and 9.3% with both yeasts simultaneously. | 1990 | 2196256 |
killer toxin of hanseniaspora uvarum. | the yeast hanseniaspora uvarum liberates a killer toxin lethal to sensitive strains of the species saccharomyces cerevisiae. secretion of this killer toxin was inhibited by tunicamycin, an inhibitor of n-glycosylation, although the mature killer protein did not show any detectable carbohydrate structures. culture supernatants of the killer strain were concentrated by ultrafiltration and the extracellular killer toxin was precipitated with ethanol and purified by ion exchange chromatography. sds- ... | 1990 | 2206104 |
yeasts associated with the white shrimp penaeus schmitti, sediment, and water of sepetiba bay, rio de janeiro, brasil. | samples were collected in a polluted site with mean fecal coliform counts of the water being 9.0 x 10(2)/100 ml and less polluted areas including commercial fishing areas with fecal coliforms typically below 2.0 x 10(1)/100 ml. yeast populations from the polluted site were 4.6 x 10(2) to 9.2 x 10(3)/g of shrimp intestine, 1.0 x 10(1) to 2.4 x 10(2)/g of sediment and 4.0 x 10(-1) to 4.6 x 10(1)/ml of water, and in the less polluted site 3.0 x 10(1) to 2.3 x 10(2)/g of intestines and 3.0 x 10(-1) ... | 1989 | 2750315 |
identification and characterization of antimicrobial activity in two yeast genera. | a general screening test for the expression of antibacterial activity was performed on over 400 cultures belonging to 31 yeast genera. of these cultures, only two, kluyveromyces thermotolerans and kloeckera apiculata, were found to produce zones of inhibition of bacterial growth on diagnostic sensitivity test agar medium supplemented with 0.002% methylene blue. of nine bacteria used as test organisms, only lactobacillus plantarum and bacillus megaterium were inhibited. no antibacterial activity ... | 1985 | 3937494 |
a microbiological assay of inositol by cup-plate method with kloeckera apiculata. | 1973 | 4796180 | |
sensitivity of heat-stressed yeasts to essential oils of plants. | eight strains of yeasts (candida lipolytica, debaryomyces hansenii, hansenula anomala, kloeckera apiculata, lodderomyces elongisporus, rhodotorula rubra, saccharomyces cerevisiae, and torulopsis glabrata) were examined for changes in sensitivity to eight essential oils of plants (allspice, cinnamon, clove, garlic, onion, oregano, savory, and thyme) after being sublethally heat stressed. with the exception of garlic oil for all test yeasts, onion oil for s. cerevisiae, and oregano oil for r. rubr ... | 1984 | 6712207 |
chemical and cytological changes during the autolysis of yeasts. | cell suspensions of sacharomyces cerevisiae, kloeckera apiculata and candida stellata were autolyzed in phosphate buffer, ph 4.5, for up to 10 days. cell dry weights decreased by 25-35% after 10 days. based on initial cell dry weight, the soluble autolysate consisted of: carbohydrate (principally polysaccharide) 3-7%; organic acids 3-6%; protein 12-13%; free amino acids 8-12%; nucleic acid products 3-5%; and lipids 1-2%. the main organic acids in autolysates were propionic, succinic and acetic a ... | 1995 | 7662285 |
the nucleotide sequence and initial characterization of pyruvate decarboxylase from the yeast hanseniaspora uvarum. | we have isolated a pyruvate decarboxylase (pdc) gene from the yeast hanseniaspora uvarum using the saccharomyces cerevisiae pdc1 gene as a probe. the nucleotide sequence of this gene was determined and compared to pdc genes from yeast and other organisms. the h. uvarum pdc gene is more than 70% identical to the s. cerevisiae pdc isozymes and possesses a putative thiamine diphosphate binding site. the pdc enzyme was purified and partially characterized. the h. uvarum pdc was very similar to other ... | 1994 | 7725793 |
glucose metabolism, enzymic analysis and product formation in chemostat culture of hanseniaspora uvarum. | the physiology of hanseniaspora uvarum k5 was studied in glucose-limited chemostat cultures and upon glucose pulse. up to a dilution rate of 0.28 h-1, glucose was completely metabolized in biomass and co2. above this value, increase in the dilution rate was accompanied by sequential production of metabolites (glycerol, acetate and ethanol) and decrease in cell yield. similar results were observed upon glucose pulse. from the enzyme activities (pyruvate dehydrogenase, pyruvate decarboxylase, nad ... | 1995 | 7785333 |
"hanseniaspora uvarum" the ultrastructural morphology of a rare ascomycete, isolated from oral thrush. | superficial fungal infections, including oral thrush, often affect aged full denture wearers and many individuals over 65 years old. the aim of this study was to examine the ultrastructural morphology of a very rare yeast, named hanseniaspora uvarum/guillermondi, member of the ascomycetes family, whose pathogenesis and behaviour is not widely known. the yeast was isolated from whitish lesions of the buccal mucosa of an 70 years old woman. the specimen was collected with a mouth swab and cultured ... | 1994 | 7994154 |
relationship between ethanol tolerance, lipid composition and plasma membrane fluidity in saccharomyces cerevisiae and kloeckera apiculata. | the lipid composition of a strain of each of two yeasts, saccharomyces cerevisiae and kloeckera apiculata, with different ethanol tolerances, was determined for cells grown with or without added ethanol. an increase in the proportion of ergosterol, unsaturated fatty acid levels and the maintenance of phospholipid biosynthesis seemed to be responsible for ethanol tolerance. the association of ethanol tolerance of yeast cells with plasma membrane fluidity, measured by fluorescence anisotropy, is d ... | 1994 | 8001764 |
characterization of beta-glucosidase activity in yeasts of oenological origin. | three hundred and seventeen strains representing 20 species of yeasts were screened for the presence of beta-glucosidase activity. all of the strains of the species debaryomyces castellii, deb. hansenii, deb. polymorphus, kloeckera apiculata and hansenula anomala showed beta-glucosidase activity, but only one of 153 strains of saccharomyces cerevisiae. the other species behaved differently, depending upon the strain. the strains that hydrolysed arbutin were checked to localize the beta-glucosida ... | 1994 | 8002477 |
killer toxin-secreting double-stranded rna mycoviruses in the yeasts hanseniaspora uvarum and zygosaccharomyces bailii. | killer toxin-secreting strains of the yeasts hanseniaspora uvarum and zygosaccharomyces bailii were shown to contain linear double-stranded rnas (dsrnas) that persist within the cytoplasm of the infected host cell as encapsidated virus-like particles. in both yeasts, l- and m-dsrnas were associated with 85-kda major capsid protein, whereas the additional z-dsrna (2.8 kb), present only in the wild-type z. bailii killer strain, was capsid protein, whereas the additional z-dsrna (2.8 kb), present o ... | 1994 | 8107238 |
the acidic ribosomal proteins of different yeast species. phosphorylation by ribosome-associated protein kinases. | two major ribosomal proteins of mr 13 kda and 38 kda were identified as phosphorylation substrates for ribosome-bound protein kinases from different yeast species. the phosphorylation level was much higher in ribosomes from the cells collected at the exponential growth phase, than in those from the stationary phase. isoelectrofocusing of the protein band of 13 kda and subsequent silver staining allowed to identify from four in the case of pichia stipitis up to ten in saccharomyces cerevisiae. sa ... | 1995 | 8537876 |
the flocculation of wine yeasts: biochemical and morphological characteristics in kloeckera apiculata. | the floc-forming ability of flocculent strains of kloeckera apiculata, isolated from musts, was tested for susceptibility to proteinase and sugar treatments. three different flocculation phenotypes were discriminated by protease digestion, whereas the inhibition of flocculation by sugars distinguished two definite patterns: one mechanism of flocculation involved a galactose-specific protein and the other a broad-specificity lectin. sem and tem observation of the cell surface of two different klo ... | 1996 | 8740910 |
plasma and erythrocyte thiamin concentrations in geriatric outpatients. | the objective of this study was a) to determine the plasma and red cell thiamin levels in geriatric outpatients and b) to evaluate, using the rat model, the sensitivity of plasma thiamin concentration as an indicator of nutritional status for this vitamin. | 1996 | 8935438 |
the effect of temperature on the growth of strains of kloeckera apiculata and saccharomyces cerevisiae in apple juice fermentation. | the influence of temperature (10 degrees c and 25 degrees c) on the survival and growth of saccharomyces cerevisiae and kloeckera apiculata was examined in mixed and pure cultures during fermentation in apple juice. the growth reached by s. cerevisiae did not seem to be affected by temperature and the presence of k. apiculata. however, the growth and survival of k. apiculata, both in single and mixed cultures, were substantially enhanced at 10 degrees c. the highest amount of ethyl acetate was p ... | 1997 | 9024002 |
secondary products formation as a tool for discriminating non-saccharomyces wine strains. strain diversity in non-saccharomyces wine yeasts. | a total of 78 strains of non-saccharomyces yeasts were isolated: 30 strains of kloeckera apiculata, 20 of candida stellata, 8 of candida valida and 20 of zygosaccharomyces fermentati. the diversity of yeast species and strains was monitored by determining the formation of secondary products of fermentation, such as acetaldehyde, ethyl acetate and higher alcohols. within each species, the strains were distinguishable in phenotypes through the production of different amounts of by-products. in par ... | 1997 | 9111917 |
the incidence of killer activity and extracellular proteases in tropical yeast communities. | the presence of killer and proteolytic yeasts was studied among 944 isolates representing 105 species from tropical yeast communities. we found 13 killer toxin producing species, with pichia kluyveri being the most frequent. other killer yeast isolates were candida apis, candida bombicola, candida fructus, candida krusei, candida sorbosa, hanseniaspora uvarum, issatchenkia occidentalis, kloeckera apis, kluyveromyces marxianus, pichia membranaefaciens, pichia ohmeri-like, and sporobolomyces roseu ... | 1997 | 9115090 |
ascomycetous yeasts associated with naturally occurring fruits in a tropical rain forest. | fruits from twenty different species of angiosperms were collected during the period from november, 1991 to january, 1992. two hundred and two strains of yeasts and yeast-like fungi were isolated, of which 74% showed ascomycetic affinity. candida was the predominant genus, followed by (in descending order of occurrence): cryptococcus, kloeckera, sporobolomyces, pichia, hanseniaspora and bullera. black yeasts and other strains showing basidiomycetic affinity were also isolated. the genus candida ... | 1997 | 9161001 |
glycerol and other fermentation products of apiculate wine yeasts. | ninety-six strains of apiculate wine yeasts were studied for their ability to produce glycerol, acetaldehyde, ethyl acetate, sulphur dioxide and hydrogen sulphide in synthetic medium. hanseniaspora guilliermondii produced smaller quantities of glycerol, acetaldehyde and hydrogen sulphide than kloeckera apiculata, whereas the production of ethyl acetate and sulphur dioxide was found to be similar. strains characterized by different capacities and properties were found for both species. the existe ... | 1997 | 9172403 |
evaluation of the biolog system for the identification of food and beverage yeasts. | the inconvenience of conventional yeast identification methods has resulted in the development of rapid, commercial systems, mainly for clinical yeast species. the biolog system (biolog inc., hayward, ca, usa) is a new semi-automated, computer-linked technology for rapid identification of clinical and non-clinical yeasts. the system is based around a microtitre tray and includes assimilation and oxidation tests. this paper evaluates the biolog system for the identification of 21 species (72 stra ... | 1997 | 9203401 |
unique double-stranded rnas responsible for the anti-candida activity of the yeast hanseniaspora uvarum. | killer strains of the yeast hanseniaspora uvarum contain cytoplasmic double-stranded rnas (dsrnas) of 4.7-kbp l and 1.0-kbp m species, which were shown to be separately packaged into icosahedral virus-like particles exhibiting rna-dependent rna polymerase activity. the l genome of the h. uvarum l-dsrna virion huv-l was shown to encode a 77-kda major capsid protein. peptide maps of the purified huv coat protein and the 81-kda major capsid protein from k1 killer viruses of saccharomyces cerevisiae ... | 1997 | 9343246 |
cloning and characterization of two pyruvate decarboxylase genes from pichia stipitis cbs 6054. | in pichia stipitis, fermentative and pyruvate decarboxylase (pdc) activities increase with diminished oxygen rather than in response to fermentable sugars. to better characterize pdc expression and regulation, two genes for pdc (pspdc1 and pspdc2) were cloned and sequenced from p. stipitis cbs 6054. aside from saccharomyces cerevisiae, from which three pdc genes have been characterized, p. stipitis is the only organism from which multiple genes for pdc have been identified and characterized. psp ... | 1998 | 9435065 |
composition of the cell walls of several yeast species. | cell walls, representing 26%-32% of the cell dry weight, were prepared from several strains of the yeasts kloeckera apiculata, debaryomyces hansenii, zygosaccharomyces bailii, kluyveromyces marxianus and saccharomyces cerevisiae. extraction of the walls with potassium hydroxide at 4 degrees c, followed by saturation of the alkali-soluble extract with ammonium sulphate gave fractions of mannoprotein, alkali-soluble glucan and alkali-insoluble glucan. chitin was associated with the alkali-insolubl ... | 1998 | 9763691 |
metabolic characterization of kloeckera apiculata strains from star fruit fermentation. | a total of 37 strains of kloeckera apiculata was isolated during the spontaneous fermentation of star fruit must. each strain was differentiated from the others on the basis of its capacity to produce acetaldehyde, ethyl acetate, higher alcohols, acetoin and acetic acid. all the strains were characterized by the low production of higher alcohols and the high production of ethyl acetate, whereas consistent differences in the production of acetaldehyde, acetoin and acetic acid served to differenti ... | 1998 | 9850418 |
isolation of hanseniaspora uvarum (kloeckera apiculata) in humans. | isolation of hanseniaspora uvarum, a yeast of the ascomycetes group, whose anamorph corresponds to kloeckera apiculata, obtained from stool and two ungual specimens from three patients, is reported. this yeast has been found in soil, water, various fruits, bivalve molluscs, crabs, prawns and fruit flies; in spain, it has been described in the fermentation processes of some wines. in our region, it has also been found in the intestine of mackerel (scomber scombrus). its finding in humans constitu ... | 1998 | 10481287 |
indigenous yeasts associated with two vitis vinifera grape varieties cultured in southern spain. | the yeast microbiota present on the surface of grapes of two vitis vinifera varieties, pedro ximénez and tempranillo de rioja, grown in the montilla-moriles region of southern spain was identified. the changes between veraison and the physiological ripeness time during 3 years were monitored. overall, the yeast microbiota isolated was of oxidative metabolism. sporobolomyces roseus and cryptococcus albidus species occurred at all physiological stages, in the two vitis vinifera varieties, and the ... | 1999 | 10582378 |
quantification of micro-organisms in binary mixed populations by fourier transform infrared (ft-ir) spectroscopy. | fourier transform infrared (ft-ir) spectroscopy was used for the first time to determine the ratios of different microorganisms in mixtures. exemplarily, systems composed of two food-associated yeast species (saccharomyces cerevisiae/hanseniaspora uvarum) and two yoghurt lactic acid bacteria (lactobacillus acidophilus/streptococcus salivarius ssp. thermophilus) were investigated. determination of the cell number ratio in the lactic acid bacteria system was possible with a minimal prediction accu ... | 2000 | 10728568 |
pesticide residues in grapes, wine, and their processing products. | in this review the results obtained in the 1990s from research on the behavior of pesticide residues on grapes, from treatment to harvest, and their fate in drying, wine-making, and alcoholic beverage processing are reported. the fungicide residues on grapes (cyproconazole, hexaconazole, kresoxim-methyl, myclobutanil, penconazole, tetraconazole, and triadimenol), the application rates of which were of a few tens of grams per hectare, were very low after treatment and were not detectable at harve ... | 2000 | 10775335 |
microbiology and physiology of cachaça (aguardente) fermentations. | cachaça (aguardente) is a rum-style spirit made from sugar cane juice by artisanal methods in brazil. a study was made of the production, biochemistry and microbiology of the process in fifteen distilleries in sul de minas. identification of 443 yeasts showed saccharomyces cerevisiae to be the predominant yeast but rhodotorula glutinis and candida maltosa were predominant in three cases. bacterial infection is a potential problem, particularly in older wooden vats, when the ratio of yeasts:bacte ... | 2001 | 11392489 |
killer toxin of kluyveromyces phaffii dbvpg 6076 as a biopreservative agent to control apiculate wine yeasts. | the use of kluyveromyces phaffii dbvpg 6076 killer toxin against apiculate wine yeasts has been investigated. the killer toxin of k. phaffii dbvpg 6076 showed extensive anti-hanseniaspora activity against strains isolated from grape samples. the proteinaceous killer toxin was found to be active in the ph range of 3 to 5 and at temperatures lower than 40 degrees c. these biochemical properties would allow the use of k. phaffii killer toxin in wine making. fungicidal or fungistatic effects depend ... | 2001 | 11425722 |
killer behaviour in wild wine yeasts associated with merlot and malbec type musts spontaneously fermented from northwestern patagonia (argentina). | the occurrence of killer wine yeasts in comahue region (patagonia, argentina) was studied. wild wine yeasts were isolated from spontaneously fermenting merlot and malbec type musts. out of 135 isolates analyzed 37% were sensitive to some well characterized killer toxins as k1-k10 and did not show killer activity (sensitive phenotype, s), 21% showed neutral phenotype (n) and 42% demonstrated killer activity (killer phenotype, k). all but two killer strains, identified as candida pulcherrima and k ... | 2001 | 11441458 |
flocculation and coflocculation of bacteria by yeasts. | biotransformations in natural environments frequently involve interactions between microorganisms. although there are many reports on the interactions between bacteria, interactions between yeasts and bacteria have not been extensively studied. previously we reported on the flocculation and coflocculation of pediococcus damnosus by saccharomyces cerevisiae. now we report that several other yeasts, such as candida utilis, dekkera bruxellensis, hanseniaspora guilliermondii, kloeckera apiculata, an ... | 2001 | 11525628 |
coflocculation of escherichia coli and schizosaccharomyces pombe. | several yeasts, such as candida utilis, dekkera bruxellensis, hanseniaspora guilliermondii, kloeckera apiculata, saccharomyces cerevisiae and schizosaccharomyces pombe, were found to coaggregate with escherichia coli, but s. pombe showed much less coflocculation than the other yeasts (peng et al. 2001)). s. pombe is known to have galactose-rich cell walls and we investigated whether this might be responsible for its different behavior by studying the wild-type tp4-1d, with a mannose to galactose ... | 2001 | 11693916 |
yeast species associated with orange juice: evaluation of different identification methods. | five different methods were used to identify yeast isolates from a variety of citrus juice sources. a total of 99 strains, including reference strains, were identified using a partial sequence of the 26s rrna gene, restriction pattern analysis of the internal transcribed spacer region (5.8s-its), classical methodology, the rapid yeast plus system, and api 20c aux. twenty-three different species were identified representing 11 different genera. distribution of the species was considerably differe ... | 2002 | 11916718 |
typing of saccharomyces cerevisiae and kloeckera apiculata strains from aglianico wine. | kloeckera apiculata and saccharomyces cerevisiae yeast species are dominant, respectively, at the early and at the following stages of wine fermentation. in the present study, pcr fingerprinting and nts region amplification and restriction were applied as techniques for monitoring yeast population performing aglianico of vulture grape must fermentation. | 2002 | 11967053 |
fermentability of grape must after inhibition with dimethyl dicarbonate (dmdc). | dimethyl dicarbonate (dmdc) was added to grape must and to synthetic media and results showed that, at 20 degrees c, 150 mg.l(-)(1) dmdc completely inhibited the fermentation of a grape must that was previously inoculated with 10(6) cells.ml(-)(1) saccharomyces bayanus and saccharomyces uvarum. brettanomyces intermedius, candida guilliermondii, hansenula jadinii, hansenula petersonii, kloeckera apiculata, pichia membranaefaciens, and saccharomyces cerevisiae were inhibited by 250 mg.l(-)(1). can ... | 2002 | 12236685 |
analysis of yeast populations during alcoholic fermentation: a six year follow-up study. | wine yeasts were isolated from fermenting garnatxa and xarel.lo musts fermented in a newly built and operated winery between 1995 and 2000. the species of non-saccharomyces yeasts and the saccharomyces cerevisiae strains were identified by ribosomal dna and mitochondrial dna rflp analysis respectively. non-saccharomyces yeasts, particularly hanseniaspora uvarum and candida stellata, dominated the first stages of fermentation. however saccharomyces cerevisiae was present at the beginning of the f ... | 2002 | 12353885 |
identification of yeast species from orange fruit and juice by rflp and sequence analysis of the 5.8s rrna gene and the two internal transcribed spacers. | yeast isolates from orange fruit and juice in a spontaneous fermentation were identified and classified by two molecular techniques. the first was analysis of the restriction pattern generated from the polymerase chain reaction (pcr)-amplified 5.8s rrna gene and the two internal transcribed spacers (its) using specific primers. the second technique was sequence analysis of the its regions using the same two primers. nine different restriction profiles were obtained from the size of the pcr produ ... | 2003 | 12702240 |
the species-specific ratios of 2,3-butanediol and acetoin isomers as a tool to evaluate wine yeast performance. | the isomers of 2,3-butanediol [r,r; s,s; r,s (meso-form)] and of acetoin (r,s) were determined in laboratory wine fermentations carried out by 50 yeast strains, 10 for each of the following species, saccharomyces cerevisiae, kloeckera apiculata, candida stellata, metschnikowia pulcherrima and brettanomyces bruxellensis, in order to evaluate if such parameters might be used to differentiate wines obtained with different yeast species. according to analysis of variance (anova), the strains of the ... | 2003 | 12892931 |
flocculation and cell surface characterization of kloeckera apiculata from wine. | to characterize and analyze the flocculation phenomenon of kloeckera apiculata mc1 from argentinian wine to understand the cell-cell interaction pattern. | 2003 | 12911692 |
hanseniaspora meyeri sp. nov., hanseniaspora clermontiae sp. nov., hanseniaspora lachancei sp. nov. and hanseniaspora opuntiae sp. nov., novel apiculate yeast species. | fourteen apiculate yeast strains isolated from various sources in south africa, north america and the hawaiian islands were found to be genetically divergent from other hanseniaspora-kloeckera species by using randomly amplified polymorphic dna (rapd)-pcr. after cluster analysis of the rapd-pcr fingerprints, five groups were recognized. dna reassociation values among representatives of these groups and strains of hanseniaspora-kloeckera species revealed that the strains represent five novel spec ... | 2003 | 13130067 |
[studies on the periodicity of types of gas exchanges in a yeast: kloeckera apiculata (rees emend. kloecker) janke]. | 1959 | 13809963 | |
activity of carbethoxythiamine and thiamine propyl disulfide on kloeckera apiculata. | 1965 | 14341426 | |
screening and typing of patagonian wine yeasts for glycosidase activities. | the purpose of this study was to select autochthonous glycosidase producer yeasts with potential use in industrial production of patagonian red wines. | 2004 | 14678162 |
yeast involved in fermentation of coffea arabica in east africa determined by genotyping and by direct denaturating gradient gel electrophoresis. | samples of coffea arabica were collected during the different stages of the fermentation from two production sites in tanzania. the yeasts community was identified by genotyping using its-pcr and sequence analysis of the d1/d2 domain of the 26s rrna gene. for confirmation, denaturating gradient gel electrophoresis (dgge) of pcr-amplified 26s rrna gene was performed to detect yeast directly from coffee samples without cultivation. yeast counts were in the range 4.0 x 10(4) - 5.0 x 10(7) cfu/g wit ... | 2004 | 15164358 |
the role of indigenous yeasts in traditional irish cider fermentations. | to study the role of the indigenous yeast flora in traditional irish cider fermentations. | 2004 | 15281947 |
molecular and technological approaches to evaluate strain biodiversity in hanseniaspora uvarum of wine origin. | the characterization by molecular and physiological methods of wild apiculate strains, isolated from 'aglianico del vulture' grape must. | 2005 | 15610426 |
mycological examinations on the fungal flora of the chicken comb. | a total of 500 combs of adult chickens from two different locations in germany (hessen and schleswig-holstein) were clinically and mycologically examined. the chickens came from three battery cages (n = 79), one voliere system (n=32), six flocks maintained on deep litter (n = 69) and 12 flocks kept on free outdoor range (n=320). twenty-two of the 500 chicken combs (4.4%) were found to have clinical signs: only non-specific lesions neither typical of mycosis nor of avian pox such as desquamation ... | 2005 | 15743428 |
chitosan inactivates spoilage yeasts but enhances survival of escherichia coli o157:h7 in apple juice. | to develop new measures for controlling both spoilage and pathogenic micro-organisms in unpasteurized apple juice using chitosan. | 2005 | 15752333 |
interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture. | applying a newly developed user-interactive optical trapping system, we controllably surrounded individual cells of one yeast species, hanseniaspora uvarum, with viable cells of another yeast species, saccharomyces cerevisiae, thus creating a confinement of the former. growth of surrounded and non-surrounded h. uvarum cells was followed under a microscope by determining their generation time. the average generation time of surrounded h. uvarum cells was 15% higher than that of non-surrounded cel ... | 2005 | 15796993 |
dynamics of indigenous yeast populations during spontaneous fermentation of wines from mendoza, argentina. | fermentation of wine is a complex microbial reaction, which involves the sequential development of various species of yeasts and lactic acid bacteria. of these, yeasts are the main group responsible for alcoholic fermentation. the aim of this work was to study, under industrial conditions, the evolution of yeast populations and to describe the individual evolution of the most important yeasts during three spontaneous fermentations of malbec musts from argentina. this work shows the significant p ... | 2005 | 15808358 |
yeasts associated to malbec grape berries from mendoza, argentina. | the aims of this work were to evaluate different pre-isolation treatments applied to complete yeast extraction from grapes and to identify the yeast microflora associated to malbec grapes from two vineyards located in mendoza, argentina. | 2005 | 15836474 |
alcohols, esters and heavy sulphur compounds production by pure and mixed cultures of apiculate wine yeasts. | strains of hanseniaspora uvarum, hanseniaspora guilliermondii and saccharomyces cerevisiae were used as pure or mixed starter cultures in commercial medium, in order to compare their kinetic parameters and fermentation patterns. in pure and mixed cultures, yeasts presented similar ethanol yield and productivity. pure cultures of h. uvarum and s. cerevisiae showed a specific growth rate of 0.38 h(-1); however, this value decreased when these yeasts were grown in mixed cultures with h. guilliermon ... | 2005 | 16099313 |
the effects of yeasts involved in the fermentation of coffea arabica in east africa on growth and ochratoxin a (ota) production by aspergillus ochraceus. | the effects of pichia anomala, pichia kluyveri and hanseniaspora uvarum predominant during coffee processing on growth of aspergillus ochraceus and production of ochratoxin a (ota) on malt extract agar (mea) and on coffee agar (ca) were studied. the three yeasts were able to inhibit growth of a. ochraceus when co-cultured in mea and ca. growth inhibition was significantly higher on mea than on ca. furthermore, p. anomala and p. kluyveri were found to have a stronger effect on growth of a. ochrac ... | 2006 | 16213049 |
influence of volatile compounds produced by yeasts predominant during processing of coffea arabica in east africa on growth and ochratoxin a (ota) production by aspergillus ochraceus. | the effects of volatile compounds produced during coffee processing by pichia anomala, p. kluyveri and hanseniaspora uvarum on growth of aspergillus ochraceus and production of ochratoxin a (ota) were studied. on malt extract agar (mea) and on coffee agar (ca), exposure of a. ochraceus to the gaseous phase of malt yeast glucose peptone (mygp) plates inoculated with p. anomala, p. kluyveri and h. uvarum inhibited fungal growth, with the two pichia spp. showing the strongest effect. the main ester ... | 2005 | 16240461 |
evolution of yeasts and lactic acid bacteria during fermentation and storage of bordeaux wines. | the levels of yeasts and lactic acid bacteria that naturally developed during the vinification of two red and two white bordeaux wines were quantitatively examined. yeasts of the genera rhodotorula, pichia, candida, and metschnikowia occurred at low levels in freshly extracted grape musts but died off as soon as fermentation commenced. kloeckera apiculata (hanseniaspora uvarum), torulopsis stellata, and saccharomyces cerevisiae, the dominant yeasts in musts, proliferated to conduct alcoholic fer ... | 1984 | 16346661 |
growth of natural yeast flora during the fermentation of inoculated wines. | the growth of yeasts that occur naturally in grape juice was quantitatively examined during the fermentation of four wines that had been inoculated with saccharomyces cerevisiae. although s. cerevisiae dominated the wine fermentations, there was significant growth of the natural species kloeckera apiculata, candida stellata, candida colliculosa, candida pulcherrima, and hansenula anomala. | 1985 | 16346894 |
analysis of yeast flora associated with grape sour rot and of the chemical disease markers. | the frequency and the density of the species associated with grape sour rot in different cultivars were determined. the most frequent species in the rotten grapes, candida krusei, kloeckera apiculata, and metschnikowia pulcherrima, and a less frequent species, issatchenkia occidentalis, when inoculated with saccharomycopsis crataegensis were able to induce in vitro the symptoms of the disease. the gas chromatographic determination of the volatile compounds in the headspace was used to evaluate t ... | 1987 | 16347305 |
dynamics of yeast populations during cider fermentation in the asturian region of spain. | the influence of different cidermaking systems and apple mixtures on the dynamics of yeast populations in cider manufactured in asturias (northern spain) were studied. results obtained in an experimental pilot plant were compared with those found in asturian cider plants by using traditional techniques. saccharomyces cerevisiae and kloeckera apiculata were found in all cases. | 1990 | 16348385 |
biometric study of acetoin production in hanseniaspora guilliermondii and kloeckera apiculata. | gas chromatographic analysis by direct injection of samples yielded quantitative data on acetoin content. ninety-six strains of hanseniaspora guilliermondii and kloeckera apiculata were investigated for the ability to produce acetoin in synthetic medium and in must. high-level production of acetoin was found to be a characteristic of both species. in synthetic medium, the two species were not significantly different with respect to sugar utilization and ethanol or acetoin production. in grape mu ... | 1993 | 16348961 |
successive microbial populations in calimyrna figs. | smyrna-type (calimyrna) figs have essentially sterile internal tissue until visited by the pollinating fig wasp, blastophaga psenes, which introduces a specific microflora consisting of candida guilliermondii var. carpophila and serratia plymuthica. this flora persists and develops in numbers throughout the ripening period until maturity of the fruit. these organisms do not cause spoilage. the presence of c. guilliermondii var. carpophila appears to increase the attractiveness of the fruit to dr ... | 1962 | 16349620 |
the mitochondrial genome of the wine yeast hanseniaspora uvarum: a unique genome organization among yeast/fungal counterparts. | the complete sequence of the apiculate wine yeast hanseniaspora uvarum mtdna has been determined and analysed. it is an extremely compact linear molecule containing the shortest functional region ever found in fungi (11 094 bp long), flanked by type 2 telomeric inverted repeats. the latter contained a 2704-bp-long subterminal region and tandem repeats of 839-bp units. in consequence, a population of mtdna molecules that differed at the number of their telomeric reiterations was detected. the fun ... | 2006 | 16423073 |
diversity and evolution of non-saccharomyces yeast populations during wine fermentation: effect of grape ripeness and cold maceration. | we have evaluated the effect of grape maturity and cold maceration prior to fermentation on the yeast ecology during wine fermentation. non-saccharomyces strains were selectively isolated and identified using two rapid pcr techniques, namely enterobacterial repetitve intergenic consensus-pcr and pcr-intron splice sites, in various wine fermentation conditions. these identifications were further complemented and confirmed by restriction fragment length poymorphism and sequencing analysis of the 5 ... | 2006 | 16423075 |
fermentation behaviour and metabolic interactions of multistarter wine yeast fermentations. | multistarter fermentations of hanseniaspora uvarum, torulaspora delbrueckii and kluyveromyces thermotolerans together with saccharomyces cerevisiae were studied. in grape musts with a high sugar content, mixed trials showed a fermentation behaviour and analytical profiles of wines comparable to or better than those exhibited by a pure culture of s. cerevisiae. sequential trials of t. delbrueckii and k. thermotolerans revealed a sluggish fermentation, while those of h. uvarum exhibited an unaccep ... | 2006 | 16487611 |
application of fluorescence in situ hybridisation (fish) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations. | to analyse the yeast population diversity during wine fermentations, specific fluorescein-labelled oligonucleotide probes targeted to the d1/d2 region of the 26s rrna of different yeast species known to occur frequently in this environment were designed and tested with reference strains. the probes were then used to identify wine must isolates and to follow, in combination with plate counts, the evolution of yeast populations in two winery fermentations of white and red grape musts. in both case ... | 2006 | 16504329 |
pectin degrading enzymes in yeasts involved in fermentation of coffea arabica in east africa. | the ability of six strains of pichia anomala, four strains of pichia kluyveri and two strains of hanseniaspora uvarum predominant during coffee processing to produce polygalacturonase (pg), pectin esterase (pe) and pectin lyase (pl) in yeast polygalacturonic acid medium (ypa) and in coffee broth (cb) was studied. for comparison, a reference strain of kluyveromyces marxianus cct 3172 isolated from cocoa and reported to produce high amount of pg was included. initial screening of pg activity using ... | 2006 | 16784790 |
in vitro interaction between ochratoxin a and different strains of saccharomyces cerevisiae and kloeckera apiculata. | the interaction of ochratoxin a (ota) and 20 yeast strains of saccharomyces cerevisiae and kloeckera apiculata during alcoholic fermentation was studied. levels of ota were determined in the fermentation liquid and in the yeast cells solid using a high-performance liquid chromatography system with a fluorescence detector. yeast cells do not adsorb ota, and for all yeasts, ota levels did not affect the alcoholic fermentation. some yeast strains reduced levels of ota, whereas other strains did not ... | 2007 | 17279767 |
purification and characterization of an alkaline protease from the marine yeast aureobasidium pullulans for bioactive peptide production from different sources. | the extracellular alkaline protease in the supernatant of cell culture of the marine yeast aureobasidium pullulans 10 was purified to homogeneity with a 2.1-fold increase in specific protease activity as compared to that in the supernatant by ammonium sulfate fractionation, gel filtration chromatography (sephadex g-75), and anion-exchange chromatography (deae sepharose fast flow). according to the sodium dodecyl sulfate-polyacrylamide gel electrophoresis data, the molecular mass of the purified ... | 2007 | 17345116 |
kinetics and metabolic behavior of a composite culture of kloeckera apiculata and saccharomyces cerevisiae wine related strains. | the kinetics and metabolic behavior of kloeckera apiculata mc1 and saccharomyces cerevisiae mc2 in composite culture was investigated. k. apiculata showed a higher viability through the fermentation; however the maximum cell density of both yeasts decreased. this behavior was not due to ethanol concentration, killer toxins production or competition for assimilable nitrogenous compounds between both yeasts. despite the consistent production of secondary products by single culture of k. apiculata, ... | 2007 | 17440687 |
diversity and killer behaviour of indigenous yeasts isolated from the fermentation vat surfaces in four patagonian wineries. | the diversity and killer behaviour of the yeast biota associated with surfaces of four patagonian wineries were analyzed in the present study. these wineries were different in their technological and ecological features. following liquid enrichment of samples from fermentation vat surfaces yeast isolates were identified by pheno- and genotyping and characterized using killer sensitivity patterns. out of 92 isolated yeasts, 25% were saccharomyces cerevisiae; 18% were kloeckera apiculata and 11% w ... | 2007 | 17531343 |
ascomycetous yeast species recovered from grapes damaged by honeydew and sour rot. | to identify ascomycetous yeasts recovered from sound and damaged grapes by the presence of honeydew or sour rot. | 2008 | 17976167 |
yeast biodiversity and dynamics during sweet wine production as determined by molecular methods. | in this study we investigated yeast biodiversity and dynamics during the production of a sweet wine obtained from dried grapes. two wineries were selected in the collio region and grapes, grape juices and wines during fermentations were analyzed by culture-dependent methods (plating on wln medium) and culture-independent methods (pcr-dgge). moreover, the capability of the saccharomyces cerevisiae starter cultures to take over the fermentation was assessed by rapd-pcr. on wln agar several species ... | 2008 | 18341578 |
modification by glucose of the flocculent phenotype of a kloeckera apiculata wine strain. | we have evaluated the induction of the flocculent phenotype of kloeckera apiculata by glucose mc1 and propose a pathway involved in carbohydrate flocculation induction. pulses of glucose were given to cells growing in glucose-poor medium (2 g l(-1)) and the flocculation percentage was measured. to elucidate the mechanism involved in flocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-dinitrophenol or camp was added to the media instead, or s ... | 2008 | 18425542 |
characterization of kinetic parameters and the formation of volatile compounds during the tequila fermentation by wild yeasts isolated from agave juice. | the production of aroma compounds during tequila fermentation using four native yeast strains isolated from agave juice was quantified at controlled (35 degrees c) and uncontrolled temperatures (room temperature) by gas chromatography (fid). three of the four strains were identified as saccharomyces cerevisiae (mtli 1, mali 1 and mgli 1) and one as kloeckera apiculata (mali 2). among the aroma compounds produced, acetaldehyde has the highest accumulation at the controlled temperature and before ... | 2008 | 18449588 |
heavy sulphur compounds, higher alcohols and esters production profile of hanseniaspora uvarum and hanseniaspora guilliermondii grown as pure and mixed cultures in grape must. | hanseniaspora guilliermondii and hanseniaspora uvarum were tested in grape must fermentations as pure and mixed starter cultures with saccharomyces cerevisiae. in pure cultures, the specific growth rates found were 0.29 h(-1) for h. uvarum, 0.23 h(-1) for h. guilliermondii and 0.18 h(-1) for s. cerevisiae. no significant differences were observed between these values and those obtained in mixed cultures. results presented in this work show that growth of apiculate yeasts during the first days of ... | 2008 | 18457893 |
dynamics and diversity of non-saccharomyces yeasts during the early stages in winemaking. | this detailed study observed the yeasts present in the ecological niche of "wine must". the dynamics and identity of non-saccharomyces yeasts during the cold maceration and alcoholic fermentation of grape must were investigated under real production conditions in the bordeaux region. furthermore, we studied the impact of two oenological parameters on the development and diversity of non-saccharomyces yeasts during cold maceration: temperature management and the timing of dried yeast addition. th ... | 2008 | 18495281 |
the effect of pyrimethanil on the growth of wine yeasts. | the toxicity of the fungicide pyrimethanil on the growth of wine yeasts was evaluated using in vivo and in vitro experimentation. | 2008 | 18544142 |
application of whole genome amplification and quantitative pcr for detection and quantification of spoilage yeasts in orange juice. | small cell numbers in complex food matrices and undefined pcr inhibitors often limit detection and identification of dna species by molecular techniques. thus in many industrial situations enrichment growths are performed. however, growth speed of different species in complex microbial mixtures in defined media is in most cases different, thus final results do not always reflect the starting situation. we tested dna-strand displacement whole genome amplification as a possible substitute of enric ... | 2008 | 18597878 |
yeast diversity in crop-growing environments in cameroon. | in the present study, we have investigated the occurrence of yeast flora on several agricultural products coming from crop-growing environments in cameroon, to provide better knowledge of the biodiversity of yeast flora, and to thus define the impact of this biodiversity on food products. the yeast biodiversity was investigated using traditional culture-dependent methods, along with culture-independent methods. the culture-dependent approach was carried out using both direct and enrichment proce ... | 2008 | 18723239 |
influence of wine-related physicochemical factors on the growth and metabolism of non-saccharomyces and saccharomyces yeasts in mixed culture. | the influence of two physicochemical factors involved in winemaking, temperature and so(2), on the kinetics and metabolic behavior of kloeckera apiculata and saccharomyces cerevisiae was examined. highest biomass was reached at 15 and 25 degrees c for k. apiculata and s. cerevisiae, respectively. pure cultures of k. apiculata died off early with increasing temperature, but in co-culture with s. cerevisiae it showed higher viability and a change in the death curve from exponential to linear. stat ... | 2009 | 18953586 |
optimising the inactivation of grape juice spoilage organisms by pulse electric fields. | the effect of some pulsed electric field (pef) processing parameters (electric field strength, pulse frequency and treatment time), on a mixture of microorganisms (kloeckera apiculata, saccharomyces cerevisiae, lactobacillus plantarum, lactobacillus hilgardii and gluconobacter oxydans) typically present in grape juice and wine were evaluated. an experimental design based on response surface methodology (rsm) was used and results were also compared with those of a factorially designed experiment. ... | 2009 | 19246114 |
growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of l. monocytogenes and c. albicans in apple-carrot juice. | food safety is a fundamental concern of both consumers and the food industry. the increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. in this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on listeria monocytogenes aufe 39237, escherichia coli ... | 2009 | 19278342 |
yeast diversity during tapping and fermentation of palm wine from cameroon. | in the present study, we have investigated the occurrence of yeast flora during tapping and fermentation of palm wine from cameroon. the yeast diversity was investigated using both traditional culture-dependent and culture-independent methods. moreover, to characterize the isolates of the predominant yeast species (saccharomyces cerevisiae) at the strain level, primers specific for delta sequences and minisatellites of genes encoding the cell wall were used. the results confirm the broad quantit ... | 2009 | 19376464 |
enhancement of survival of probiotic and non-probiotic lactic acid bacteria by yeasts in fermented milk under non-refrigerated conditions. | the effects of yeasts on the survival of probiotic and non-probiotic lactic acid bacteria (lab) were studied in fermented milk under non-refrigerated conditions (30 degrees c) with a view to develop ambient-stable fermented milk with live lab. five yeasts tested (saccharomyces bayanus, williopsis saturnus var. saturnus, yarrowia lipolytica, candida kefyr and kluyveromyces marxianus) enhanced the survival of lactobacillus bulgaricus (but not streptococcus thermophilus) in a mixed yoghurt culture ... | 2009 | 19666198 |
yeasts isolated from a fungus-growing ant nest, including the description of trichosporon chiarellii sp. nov., an anamorphic basidiomycetous yeast. | thirty-nine yeast strains were recovered from a field nest of a primitive and putative novel attine ant species in the genus myrmicocrypta (hymenoptera: formicidae: tribe attini). yeasts isolated from the fungus garden and waste deposit included candida dubliniensis, candida oleophila, cryptococcus haglerorum and hanseniaspora uvarum. in addition, one morphological type was isolated overwhelmingly. sequencing data of partial large-subunit (lsu) rdna and the internal transcribed spacer (its) regi ... | 2010 | 19671730 |
differential response of pichia guilliermondii spoilage isolates to biological and physico-chemical factors prevailing in patagonian wine fermentations. | pichia guilliermondii can produce volatile phenols in the initial stages of wine fermentation; however, its response to different wine stress conditions has been poorly studied. in this work, we present an analysis of the response of 23 p. guilliermondii indigenous isolates to physical and chemical wine stress factors and to indigenous wine killer yeasts. principal coordinates analysis (pcoa), based on data obtained from response patterns, was carried out to evaluate the relationships among the ... | 2009 | 19767852 |
natural yeast flora of different varieties of grapes used for wine making in india. | the natural saccharomyces and non-saccharomyces yeast flora present on the grape berries significantly affect wine production. six grape varieties, bangalore blue, zinfandel, cabernet, chenin blanc, sauvignon blanc and shiraz are being used in india for wine making. the yeast diversity was studied on the basis of morphological, colony, physiological characteristics and 5.8s-its sequencing of rdna of the isolates. eleven different species belonging to seven genera were identified as: candida azym ... | 2009 | 19835764 |
the zymocidial activity of tetrapisispora phaffii in the control of hanseniaspora uvarum during the early stages of winemaking. | the yeast strain tetrapisispora phaffii dbvpg 6706 (formerly kluyveromyces phaffii) secretes a killer toxin (kpkt) that has antimicrobial activity against apiculate yeasts. the aim of this study was to evaluate the killer activity of kpkt towards hanseniaspora uvarum under winemaking conditions. | 2010 | 19874485 |
yeast species associated with wine grapes in china. | having more information on the yeast ecology of grapes is important for wine-makers to produce wine with high quality and typical attributes. china is a significant wine-consuming country and is becoming a serious wine-producer, but little has been reported about the yeast ecology of local ecosystems. this study provides the first step towards the exploitation of the yeast wealth in china's vine-growing regions. the aim of this study was to investigate the yeast population density and diversity ... | 2010 | 20116124 |
influence of indigenous yeasts on the fermentation and volatile profile of plum brandies. | the aim of this study was to determine the influence of different yeasts isolated from fresh blue plum fruits (aureobasidium sp.) and spontaneously fermenting plum musts (kloeckera apiculata and saccharomyces cerevisiae), as well as commercial wine and distillery strains, on the fermentation and chemical composition of plum brandies. gas chromatography methods were used to detect major volatile components. the most rapid fermentation occurred in musts inoculated with s. cerevisiae. however, the ... | 2010 | 20227608 |
genetic and phenotypic diversity of autochthonous cider yeasts in a cellar from asturias. | this paper analyses yeast diversity and dynamics during the production of asturian cider. yeasts were isolated from apple juice and at different stages of fermentation in a cellar in villaviciosa during two asturian cider-apple harvests. the species identified by its-rflp corresponded to hanseniaspora valbyensis, hanseniaspora uvarum, metschnikowia pulcherrima, pichia guilliermondii, candida parapsilosis, saccharomyces cerevisiae and saccharomyces bayanus/saccharomyces pastorianus/saccharomyces ... | 2010 | 20417399 |