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further analyses of the orthopoxviruses volepox virus and raccoon poxvirus.volepox virus (vpx) from skin lesions on a vole and a piñon mouse caught in california and raccoon poxvirus (rcn) from raccoons trapped in maryland were examined to begin elucidating their relationship to other orthopoxviruses, most of which are not known to be indigenous to the americas. vpx and rcn produced pinpoint, nonhemorrhagic pocks on chick embryo chorioallantoic membranes. in cell cultures both viruses produced 1-mm diameter, irregular plaques, a-type inclusions (atis), and despite prod ...19921529541
sequences of the raccoon poxvirus hemagglutinin protein.primers based on sequences flanking the vaccinia virus (vv) strain ihd hemagglutinin protein (ha) open reading frame (orf) enabled amplification of ha dna segments from the genome of raccoon poxvirus (rcn) and vv strain wr. the amplified segments produced unequal cross-hybridization signal intensities against each other, indicating sequence differences between the ha of rcn (in hindiii-g) and that of vv-wr (in hindiii-a). about 1.5 kb of sequences in the ha region were then determined from clone ...19921529542
detection of north american orthopoxviruses by real time-pcr.the prevalence of north american orthopoxviruses in nature is unknown and may be more difficult to ascertain due to wide spread use of vaccinia virus recombinant vaccines in the wild. a real time pcr assay was developed to allow for highly sensitive and specific detection of north american orthopoxvirus dna in animal tissues and bodily fluids. this method is based on the amplification of a 156 bp sequence within a myristylated protein, highly conserved within the north american orthopoxviruses b ...201121689420
the pox in the north american backyard: volepox virus pathogenesis in california mice (peromyscus californicus).volepox virus (vpxv) was first isolated in 1985 from a hind foot scab of an otherwise healthy california vole (microtus californicus). subsequent surveys in san mateo county, ca, revealed serological evidence suggesting that vpxv is endemic to this area, and a second viral isolate from a pinyon mouse (peromyscus truei) was collected in 1988. since then, few studies have been conducted regarding the ecology, pathology, and pathogenicity of vpxv, and its prevalence and role as a potential zoonotic ...201222952799
endemic orthopoxvirus circulating in procyonids in mexico.limited serosurveillance studies suggested that orthopoxviruses (opxv) are widespread in the us (e.g., raccoonpox virus, skunkpox virus, volepox virus) and brazil (vaccinia virus); however, their animal reservoir(s) remain unconfirmed. mexican mammal diversity includes several species related to those in which evidence for opxv infections has been found (oryzomys, peromyscus, microtus, and procyonidae). the presence of these groups of mammals in mexico and the evidence of their possible involvem ...201627224209
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