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light and electron microscopic observations on encystment of acanthamoeba palestinensis, reich.the ultrastructural changes occurring during encystment of acanthamoeba palestinensis have been investigated. the cyst wall consists of endocyst and exocyst, both having the same fine structure. at irregular intervals in the cyst wall ostioles occupied by opercula are present. the nuclear membrane forms bulb-shaped projections and releases vesicles bounded by double membranes into the cytoplasm. dense nucleolus-like bodies of different sizes and variable numbers are found in the nucleus of every ...1977881652
molecular aspects of the cell cycle and encystment of acanthamoeba.evidence for subdivision of the cell cycle of acanthamoeba into ultradian biochemical cycles is accumulating, and a linkage between these cycles and the length of the cell cycle is possible. the dna replication cycle differs with the method of assay: no g1 phase is found in asynchronous cultures, and a long g1 phase is found in synchronous cultures. encystment most likely occurs from g2, but whether it is limited to a portion of this phase is not clear. encystment-enhancing factors are released ...19912047666
growth and survival of four strains of francisella tularensis in a rich medium preconditioned with acanthamoeba palestinensis.the hypothesis of positive interactions between francisella tularensis lvs (live vaccine strain) and acanthamoeba palestinensis was tested. pregrowth of the amoebae, in a rich (autoclaved and filtered) medium, from which they were subsequently removed by filtration, conditioned the medium so that growth of the live vaccine strain of f. tularensis occurred and the growth rate of one other strain was increased.19892630032
activation of adenylate cyclase in acanthamoeba palestinensis.preincubation of acanthamoeba palestinensis homogenates in 0.25m sucrose-tm (2mm mgso4 and 5mm tris-hcl, ph 7.4) at 0 degree c for increasing periods of time up to 3 h, leads to a progressive increase in the activity of adenylated cyclase. in contrast, preincubation of isolated membrane fractions enriched in enzyme activity in the same medium results in no activation. however, preincubation of membrane fractions in medium containing a high density of sugars (sucrose, glucose or fructose) mimics ...19863005797
assembly of lipids into membranes in acanthamoeba palestinensis. ii. the origin and fate of glycerol- 3 h--labeled phospholipids of cellular membranes.the membranes of acanthamoeba palestinensis were studied by examination in fixed cells, and then by following the movements of glycerol-(3)h-labeled phospholipids by cell fractionation. two previously undescribed structures were observed: collapsed cytoplasmic vesicles of cup shape, and plaques in food vacuole and plasma membrane similar in size to the collapsed vesicles. it appeared that the plaques formed by insertion of collapsed vesicles into membranes and/or that collapsed vesicles formed b ...19714329152
subcellular distribution of adenyl cyclase and phosphodiesterase in acanthamoeba palestinensis. 19734350748
assembly of lipids into membranes in acanthamoeba palestinensis. i. observations on the specificity and stability of choline- 14 c and glycerol- 3 h as labels for membrane phospholipids.in order to determine the feasibility of using radioactive precursors as markers for membrane phospholipids in acanthamoeba palestinensis, the characteristics of phospholipids labeled with choline-(14)c and glycerol-(3)h were examined. choline-(14)c was found to be a specific label for phosphatidyl choline. there was a turnover of the radioactive moiety of phosphatidyl choline at a rate that varied with the concentration of nonradioactive choline added to the growth medium. radioactivity was los ...19715098863
regularly arranged particles on the inner lamella of the plasma membrane of acanthamoeba palestinensis. 19715128357
interaction of l. pneumophilia and a free living amoeba (acanthamoeba palestinensis).co-cultivation of legionella pneumophila serogroup i and acanthamoeba palestinensis in neff's medium at 35 degrees c resulted in the intracellular multiplication of the bacteria as demonstrated by electron microscopy and immunofluorescence. in the closed experimental system used, the number of legionellae rose from 10(7) colony forming units (c.f.u.)/ml initially to a maximum of 10(10) c.f.u./ml on day 5. legionellae were seen in expelled phagosomes, in some amoebae filling the cytoplasm and in ...19836358342
control of cryptococcus neoformans in nature by biotic factors.two bacterial species (pseudomonas aeruginosa and bacillus subtilis) isolated from pigeon droppings, displayed anti cryptococcus neoformans activity on 4 of 6 media and sterilized pigeon droppings. acanthamoeba palestinensis trophozoites isolated from pigeon droppings ingested and killed 99.9% of c neoformans cells after 7 days of incubation. mites and sow bugs (metoponorthus pruinosus) isolated from pigeon droppings appear to be fungivorous. these findings suggest that many organisms that occur ...19826801787
subgenus systematics of acanthamoeba: four nuclear 18s rdna sequence types.classification of acanthamoeba at the subgenus level has been problematic, but increasing reports of acanthamoeba as an opportunistic human pathogen have generated an interest in finding a more consistent basis for classification. thus, we are developing a classification scheme based on rna gene sequences. this first report is based on analysis of complete sequences of nuclear small ribosomal subunit rna genes (rns) from 18 strains. sequence variation was localized in 12 highly variable regions. ...19968976608
close relatedness of acanthamoeba pustulosa with acanthamoeba palestinensis based on isoenzyme profiles and rdna pcr-rflp patterns.the taxonomic validity of morphological group iii acanthamoeba spp. is uncertain. in the present study, six type strains of group iii acanthamoeba spp., a. culbertsoni, a. healyi, a. pustulosa, a. palestinensis, a. royreba and a. lenticulata were subjected for the evaluation of their taxonomic validity by comparison of the isoenzyme patterns by isoelectic focusing on polyacrylamide gels, mitochondrial dna (mt dna) restriction fragment length polymorphism (rflp), and small subunit ribosomal dna ( ...19969017912
flow cytometric determination of endocytosis of viable labelled legionella pneumophila by acanthamoeba palestinensis.endocytosis of fluorescently labelled cells of legionella pneumophila (l. pneumophila) by free-living acanthamoeba palestinensis (a. palestinensis) has been studied using flow cytometry. l. pneumophila cells were labelled with cm-dii, a lipophilic fluorescent probe under conditions that did not modify viability. coculturing the bacteria with amoebae was accompanied by rapid endocytosis; after 5 min, 90% of the amoebae had internalized bacteria. this percentage remained unchanged during further c ...19979041116
[therapy refractory keratitis. contact lens-induced keratitis caused by acanthamoeba palestinensis]. 19979312323
phthalocyanine-photosensitized inactivation of a pathogenic protozoan, acanthamoeba palestinensis.incubation of acanthamoeba palestinensis cells with a tetracationic phthalocyanine (rlp068) at concentrations ranging between 0.2 and 1.0 microm, caused a ready uptake of the photosensitizer with recoveries of the order of 0.5-2.5 nmol per mg of cell protein. the amount of cell-bound phthalocyanine did not appreciably change with incubation times ranging between 0.5 and 3 h. fluorescence microscopic investigations showed an obvious accumulation of the phthalocyanine at the level of the vacuolar ...200312859151
photosensitized inactivation of acanthamoeba palestinensis in the cystic stage.to develop alternative approaches for medical and environmental control of pathogenic acanthamoeba spp. by means of photodynamic treatment with a tetracationic zn(ii)-phthalocyanine (rlp068).200616834608
interactions between food-borne pathogens and protozoa isolated from lettuce and spinach.the survival of salmonella enterica was recently shown to increase when the bacteria were sequestered in expelled food vacuoles (vesicles) of tetrahymena. because fresh produce is increasingly linked to outbreaks of enteric illness, the present investigation aimed to determine the prevalence of protozoa on spinach and lettuce and to examine their interactions with s. enterica, escherichia coli o157:h7, and listeria monocytogenes. glaucoma sp., colpoda steinii, and acanthamoeba palestinensis were ...200818310421
mechanisms involved in the photosensitized inactivation of acanthamoeba palestinensis trophozoites.to advance our understanding of the mechanisms involved in the rlp068 phthalocyanine-photosensitized inactivation of acanthamoeba palestinensis trophozoites through a precise identification of the targets of the photoprocess in both the cytosolic and mitochondrial compartments.200919457022
length and sequence heterogeneity in the mitochondrial internal transcribed spacer of acanthamoeba spp.the internal transcribed spacer (its) between the mitochondrial large (23s rrna; rnl) and small (16s rrna; rns) subunit ribosomal rna genes of acanthamoeba castellanii strain neff was sequenced previously and was uniquely interesting because it contained trna genes with acceptor stem mismatches that underwent rna editing repair. our interest in this its region was to determine its phylogenetic potential in differentiating between closely related isolates. we analyzed the mitochondrial its region ...200919527353
molecular identification and sequencing of mannose binding protein (mbp) gene of acanthamoeba palestinensis.acanthamoeba keratitis develops by pathogenic acanthamoeba such as a. palestinensis. indeed this species is one of the known causative agents of amoebic keratitis in iran. mannose binding protein (mbp) is the main pathogenicity factors for developing this sight threatening disease. we aimed to characterize mbp gene in pathogenic acanthamoeba isolates such as a. palestinensis.201022347229
diversity and seasonal impact of acanthamoeba species in a subtropical rivershed.this study evaluated the presence of acanthamoeba species in the puzih river watershed, which features typical subtropical monsoon climate and is located just above the tropic of cancer in taiwan. the relationship between the seasonal and geographical distributions of acanthamoeba species in this rivershed was also investigated. acanthamoeba species were detected in water samples using the amoebal enrichment culture method and confirmed by pcr. a total of 136 water samples were included in this ...201324490160
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