Publications

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the efficacy of showers for control of ectoparasites of sheep.ectoparasites of sheep in britain include the tick ixodes ricinus, the blowfly, lucilia sericata, the ked, melophagus ovinus and the lice, damalinia ovis and linognathus ovillus. the most important ectoparasite, however, is the mite psoroptes communis ovis which causes sheep scab. this notifiable disease was eradicated from britain in 1952 but was reported again in 1973. the control of ectoparasites depends largely on the efficient application of insecticide to the fleece and skin. plunge dippin ...197876363
the relationship between visual acuity and illumination in the fly, lucilia sericata. 1978149447
the amino acid sequence of cytochrome c from the blowfly lucilia cuprina.the amino acid sequence of cytochrome c isolated from the sheep blowfly lucilia cuprina has been determined by comparison of the compositions of the tryptic peptides to those predicted from the published sequences of cytochromes c from other insects. cytochrome c from l. cuprina differs at a single residue when compared to cytochrome c from the screw worm fly haematobia irritans, a species belonging to the same order as the blowfly. this substitution, proline for alanine, has been located at pos ...1978202328
[facultative wound myiasis of man in austria (author's transl)].three cases of contingent wound myiasis (traumatic myiasis) in man in austria caused by lucilia sericata (2 cases) and sarcophaga argyrostoma and lucilia sericata (1 case) are reported.1979463038
sheep blowfly breech strike control using aluminium alkoxide gellants. 1978569387
changes in the response of mitochondrial calcium transport to exogenous phosphate during development in flight muscle of the sheep blowfly lucilla cuprina.1. ca2+ transport by mitochondria isolated from flight muscle of the sheep blowfly lucilla cuprina does not occur in the absence of added p1. maximum rates of transport are attained when about 2.5 mm-phosphate is present. 2. as mitochondria develop, high but not low phosphate concentrations begin to inhibit ca2+ transport markedly; those isolated from 2-day-old flies for example, are inhibited by about 75% by 20 mm-phosphate. maximum rates of transport, i.e. those measured in the presence of 2.5 ...1978629784
the role of non-biting flies in the transmission of enteric pathogens (salmonella species and shigella species) in beirut, lebanon.in the summer and early autumn of 1974, flies were trapped in the beirut districts of ashraffiyah, burj, burj-hammoud, quarantine, the new slaughter house, ras beirut, american university campus, and the suburbs of chiyah and mreyjieh. musca domestica, muscina stabulans, calliphora vicina, phormia regina, phaenicia sericata, sarcophagidae spp. and fannia canicularis were identified. the rural areas of mreyjieh and chiyah had higher densities of musca domestica while higher densities of calliphor ...1978678345
inability of tributyltin-induced chloride/hydroxyl exchange to stimulate calcium transport in mitochondria isolated from flight muscle of the sheep blowfly lucilia cuprina.tributyltin in the concentration range 1-4mum failed to stimulate ca(2+) transport by lucilia flight-muscle mitochondria in a medium containing kcl and respiratory substrate but devoid of p(i), despite its promotion of a rapid cl(-)/oh(-) exchange. when 2mm-p(i) was present, concentrations of tributyltin greater than 1mum inhibited the initial rate of ca(2+) transport and induced efflux of the ion from the mitochondria in cl(-)- or no(3) (-)-containing media. lower concentrations had little effe ...1978728076
xanthommatin biosynthesis in wild-type and mutant strains of the australian sheep blowfly lucilia cuprina.the synthesis of eye pigments has been studied in the seven eye color mutants of the australian sheep blowfly, lucilia cuprina. six appears to be affected primarily in the synthesis of xanthommatin. in wild type, the onset of xanthommatin biosynthesis occurs midway through metamorphosis. developmental patterns of accumulation of the xanthommatin precursors tryptophan, kynurenine, and 3-hydroxykynurenine have also been established for wild type. by determining the levels of these precursors in la ...1978751645
evidence of a calcium-ion-transport system in mitochondria isolated from flight muscle of the developing sheep blowfly lucilia cuprina.the egta (ethanedioxybis(ethylamine)tetra-acetic acid)-ruthenium red-quench technique (reed & bygrave, 1974a) was used to measure initial rates of ca-2+ transport in mitochondria from flight muscle of the blowfly lucilia cuprina. evidence is provided for the existence in these mitochondria of a ca-2+-transport system that has many features in common with that known to exist in rat liver mitochondria. these include requirement for energy, saturation at high concentrations of ca-2+, a sigmoidal re ...1975807204
[living larvae of the fly lucilia sericata in the tympanic cavity after atticoantrotomy]. 1977890834
lack of persistence in the haemolymph accounts for the low toxicity of the putative transmitter l-glutamate towards the sheep maggot fly lucilia sericata [proceedings]. 1977913787
dietary-induced modification of calcium transport in mitochondria isolated from flight-muscle of developing sheep blowfly lucilia cuprina. 1977921793
the synthesis of compound autosomes in the australian sheep blowfly lucilia cuprina.the synthesis of the first compound chromosome strain in lucilla cuprina (diptera: calliphoridae) (weidemann) is described. crosses between the compound chromosome strain and a chromosomally normal strain produce no offspring, whereas crosses between compound chromosome-bearing individuals are fertile. cytological evidence is presented that compound chromosome arms assort art random with respect to one another during spermatogenesis.1976938976
a survey of houseflies in bangkok and neighboring provinces.a survey of flies in bangkok and neighboring province revelaed that among 8,675 flies collected there were 10 genera and 18 species of flies, eg. musca domestica, m. bezzii, m. conducens, m. nebulo, chrysomyia megacephala, c. rufifacies, phaenicia sericata, p. cuprina, sarcophaga ruficornis, s. peregrina, s. orchidae, s. albiceps, megaselia scalaris, lispe orientalis, anaclysta flexa, morellia hortensia, hemipyrellia ligurriens and orthellia coeruleifrons. musca mostly bred in low-protein conten ...19761027114
[wound myiasis caused by lucilia sericata].report of a case of miyasis in lucilia sericata infected wounds recently observed in paris in a 67-year-old vagrant. the authors describe the role played by lucilia sericata in therapy before the appearance of sulfanilamides and antibiotics.19761037094
isolation and characterization of ribosomes from thoraces of the sheep blowfly, lucilia cuprina, and their capacity for protein synthesis at different stages of development. 19751116644
synchronous appearance of adenine nucleotide translocase activity and oxidative phosphorylation in mitochondria from flight-muscle of the developing sheep blowfly, lucilia cuprina. 19751132511
[human myiasis in middle europe].a report is given on 4 cases of myiasis of the mucous membrane of different localisation and etiology observed in the gdr (vaginal myiasis by lucilia sericata, urethral and vesicular myiasis by calliphora vicina, rectal myiasis by sarcophaga haemorrhoidalis and ophthalmomyiasis by oestrus ovis). biological peculiarities and habits of life of the causative organisms, pathogenesis of the different forms of parasitosis in man and clinical pictures are described. the cases mentioned show that occasi ...19751163070
[human umbilical myiasis by phaenicia sericata in a newborn child (author's transl)]. 19751174420
the effect of inhibitors in vivo on protein synthesis and the amino acid pool in the sheep blowfly, lucilia cuprina.1. the rates of detoxification of cycloheximide (33 mug/g fresh wt.), puromycin (167 mug/g fresh wt.) and actinomycin d (1 mug/g fresh wt.) were assessed in vivo on the basis of acid-insoluble [14c]leucine incorporation in the sheep blowfly, lucilla cuprina; these were compared with quantitative estimates which took account not only of incorporation data but also of leucine pool size and turnover. quantitatively, cycloheximide and puromycin were still at least 50% effective in inhibiting protein ...19751180920
resistance of the sheep blowfly lucilia cuprina to insecticides in the republic of south africa.laboratory tests on larvae of nine strains of the sheep blowfly, lucilia cuprina, and one of the hairy blowfly, chrysomia albiceps, taken from the field are reported. results indicate resistance to arsenic in one strain and to diazinon in the remainder and in chrysomia albiceps. field evidence corroborates the presence of resistance in the blowfly maggots to current flystrike insecticides with reduced protection resulting in more frequent treatment.19751223284
an assessment of the practical impact of insecticide resistance.in spite of widespread resistance of the sheep blowfly to organophosphorus insecticides, they are still widely relied upon in austrialia. hybrid-resistant blowfly larvae do not produce fly-strike as readily as pure-resistant larvae so more attention should be paid to the use of biological, rather than chemical, control, wherever possible.19761258296
genetics of lucilin, a storage protein from the sheep blowfly, lucilia cuprina (calliphoridae).lucilin, the main storage protein of larval fat body and hemolymph in the sheep blowfly, lucilia cuprina, has been isolated as a series of trimers composed of subunits of 83,000 +/- 5%, daltons. extensive electrophoretically detectable polymorphism of lucilin subunit patterns occurs in wild and laboratory populations of lucilia; from four to nine bands are seen in any one individual. evidence from genetic, electrophoretic, immunological, and structural studies suggests the existence of a series ...19761259701
reduced growth of lucilia cuprina larvae fed serum from sheep treated with anthelmintics.the effect of three commonly used anthelmintics, levamisole hydrochloride, ivermectin and closantel, on the development of the sheep blowfly, lucilia cuprina, was determined. sheep were treated with each anthelmintic using the manufacturers' recommended dose for helminth control. both ivermectin and closantel significantly (p < 0.05) reduced the growth rate of larvae of l cuprina cultured in vitro on serum from these sheep. levamisole hydrochloride had no effect. ivermectin was effective for les ...19921288476
genomic sequences with homology to the p element of drosophila melanogaster occur in the blowfly lucilia cuprina.we have cloned two dna elements (lu-p1 and lu-p2) from the australian sheep blowfly lucilia cuprina that are similar to the transposable p element of drosophila melanogaster in both structure and sequence but have diverged from it and from each other considerably. hybridization studies indicate that a third related element probably exists in another, as yet unsequenced, clone. neither lu-p1 nor lu-p2 appears to be active in terms of mobility, and it is not known whether any transposition-compete ...19921332056
the nss mutation or lanthanum inhibits light-induced ca2+ influx into fly photoreceptors.ion-selective calcium microelectrodes were inserted into the compound eyes of the wild-type sheep blowfly lucilia or into the retina of the no steady state (nss) mutant of lucilia. these electrodes monitored light-induced changes in the extracellular concentration of calcium (delta[ca2+]o) together with the extracellularly recorded receptor potential. prolonged dim lights induced a steady reduction in [ca2+]o during light in the retina of normal lucilia, while relatively little change in [ca2+]o ...19921335476
fatal association between dieldrin-resistant and susceptible australian sheep blowflies, lucilia cuprina.a novel phenomenon of interactions between genotypes of the dieldrin-resistance (rdl) locus of the australian sheep blowfly (lucilia cuprina) is described. susceptible adult flies exposed to dieldrin-resistant (rdl/rdl or rdl/s) adults, raised from larvae grown on media containing sublethal concentrations of dieldrin, display mortality related to the concentration on which the resistant flies developed. the resistant flies excrete quantities of dieldrin that are toxic to susceptible flies. these ...19921349179
vaccine r & d at csiro's division of tropical animal production.the extensive research on animal vaccines being undertaken by the csiro division of tropical animal production in brisbane is summarised in this paper. much of the research is being developed in conjunction with commercial partners. vaccines are being developed for the cattle tick, babesia, anaplasma, sheep blowfly, buffalo fly and bovine ephemeral fever.19911367735
gold labelling of rna in virus-induced mitochondrial vesicles in the sheep blowfly lucilia cuprina.membranous vesicles are thought to be the replication site for viral rna of many plant and animal viruses. a relatively rare site of virus-associated vesiculation is that of the mitochondrial outer membrane. in this study, virus-induced mitochondrial vesicles of the blowfly, lucilia cuprina, were labelled with ribonuclease/gold and an antibody against double stranded rna (anti-polyinosinic:polycytidylic acid). both methods showed the presence of rna in the vesicles thus indicating they may be a ...19921378980
cellular immune responses in the skin of sheep infected with larvae of lucilia cuprina, the sheep blowfly.cellular immune responses were observed in the skin of sheep after primary and secondary infection with sheep blowfly (lucilia cuprina) larvae. both primary and secondary infections resulted in a massive cellular infiltration within 48 h of wound initiation, with the majority of cells having the cd45 phenotype. neutrophils comprised the major cell type at the skin surface. in the dermis, the number of cd4+ t helper, gamma delta-tcr+ cells and t19+ (cd4-, cd8-) t cells also increased significantl ...19921441187
potent immunosuppression by secretory/excretory products of larvae from the sheep blowfly lucilia cuprina.secretory/excretory products (sec/ex) of parasitic larvae of the sheep blowfly lucilia cuprina potently inhibited proliferation of peripheral blood leucocytes stimulated by mitogens in vitro. suppression of proliferation was not due to irreversible damage because cells cultured for 24 h in high concentrations of sec/ex appeared viable (assessed by trypan blue exclusion) and did not show impaired proliferation after washing. furthermore, suppression induced by sec/ex could be overcome by increasi ...19921470479
hypersensitivity responses and repeated infections with lucilia cuprina, the sheep blowfly.sheep repeatedly infected with l. cuprina at 2- but not 4-week intervals developed partial resistance to infection after five infections, as measured by larval recovery. however, resistance did not persist for more than three infections. skin weal responses were measured after injection of larval products simultaneously with each infection. the only correlation between weal size and larval recoveries occurred at infection 1 and indicated a relationship between skin sensitivity and innate rather ...19921487378
[mixed semi-specific cutaneous myiasis].a case of mixed semi-specific cutaneous myiasis produced by larvae from calliphora vicina rob.-desv. and lucilia sericata mg, in their third development stage, is described, as the first case in humans, although there is a precedent described in domestic animals. main taxonomic characteristics and diagnostic features of the parasites are discussed, together with the implications of their biology in the observed clinical case, discussing, as well the prophylaxis and therapy.19921502404
characterization and chromosomal distribution of a tandemly repeated dna sequence from the australian sheep blowfly, lucilia cuprina.in the course of making a lucilia cuprina genomic dna library, a ladder of bands was seen in partial sau3a digests. complete digestion reduced this ladder to predominantly monomer units of approximately 190 bp. nine independently isolated copies of this repeat were cloned and sequenced. only two of these isolates are identical in sequence, the most divergent being 71% homologous. this satellite dna occurs in all three wild-type strains tested, and, for the single case examined, in the embryonic, ...19921576886
retarded growth of lucilia cuprina larvae on sheep and their sera following production of an immune response.attempts were made to immunize sheep against larvae of the sheep blowfly lucilia cuprina using supernatant and pellet prepared by centrifuging (100,000 g max) homogenates from whole second instar larvae of l. cuprina or their excised guts. injection of supernatant from whole larvae and from two fractions of this supernatant, prepared by ammonium sulphate precipitation, significantly reduced (by 24-58%) the final weight of larvae grown in vivo (i.e. on immunized sheep) for 20 or 44 h. serum from ...19921587682
development of an attractive target for the sheep blowfly lucilia sericata.coloured, odour-baited, sticky targets, designed to catch the sheep blowfly lucilia sericata meigen are described and the results of field trials are presented. the number of l. sericata caught by the targets was positively related to target surface area and was dependent on target colour. the performance of the coloured targets is described by a model in which catch is related negatively to reflectivity in the 300-450 nm (ultra-violet/blue) band of the spectrum and positively to the reflectivit ...19921600231
polytene chromosomes of the old world screwworm fly (chrysomya bezziana) and its evolutionary relationships with lucilia cuprina and cochliomyia hominivorax (diptera: calliphoridae).standard polytene chromosome maps for the old world screwsworm fly, chrysomya bezziana, are presented. good quality polytene chromosomes obtainable from pupal trichogen cells allow detailed analysis of autosomal euchromatin. the sex chromosomes are represented by irregular heterochromatic structures resembling those described previously in trichogen polytene chromosomes of the australian sheep blowfly, lucilia cuprina. a high degree of homology with the banding pattern of l. cuprina polytene chr ...19921618389
a simple larval diet for population studies on the blowfly lucilia sericata (diptera: calliphoridae).1. a simple artificial diet was devised for larvae of lucilia sericata meigen. 2. a basic diet of 20 g/l agar with 20% horse blood and added yeast sustained normal growth and development, as compared with a lamb meat control. 3. when yeast was added to the blood agar diet at 50 g/l, both peak and final larval weights were increased by 25-50% at higher larval densities (2-8 larvae/g diet) and the hatchling-adult development period was reduced by about 3 days. 4. no adult insects emerged on an aga ...19911768921
purified glutathione s-transferases from parasites as candidate protective antigens.glutathione s-transferase (gst) activity was detected in larvae of the australian sheep blowfly lucilia cuprina, and in the nematode haemonchus contortus. a specific inhibitor of the enzyme was shown to affect survival of both species of parasite in vitro. gst from both parasites has been purified and partially characterized. antisera raised to the purified enzymes were shown to inhibit the enzyme activity in vitro. however, the antisera had no effect on the survival of either parasite.19911774119
male crossing over and genetic sexing systems in the australian sheep blowfly lucilia cuprina.field-female killing (fk) systems based on deleterious mutations and y-autosome translocations are being evaluated for genetic control of the australian sheep blowfly, lucilia cuprina. experience during field trials has shown that mass-reared colonies of fk strains are subject to genetic deterioration, caused mainly by genetic recombination in males. a previous study found higher male recombination frequencies in two y-linked translocation strains than in chromosomally normal males. however, the ...19911774192
polar glycosphingolipids in insect: chemical structures of glycosphingolipid series containing 2'-aminoethylphosphoryl-(----6)-n-acetylglucosamine as a polar group from larvae of the green-bottle fly, lucilia caesar.a series of glycosphingolipids containing 2'-aminoethylphosphoryl(----6)-n-acetylglucosamine as a polar group has been demonstrated in larvae of the green-bottle fly, lucilia caesar. the thin-layer chromatographic pattern of the total polar glycolipid revealed the presence of more than eight components, of which five major components were purified by the use of successive column chromatography on qae- and deae-sephadex and silicic acid (iatrobeads). from structural studies including compositiona ...19911778972
[synanthropic flies in an 11-story apartment house in gdaƄsk].about 2500 specimens belonging to diptera were collected. the family muscidae was most numerously represented with 51.6% of the flies caught. 5 of the 18 species were dominant: fannia canicularis, muscina stabulans, lucilia sericata, protophormia terraenovae, calliphora vicina. comparison of the material from the 3 localities showed up differences in both qualitative and quantitative content.19911823470
some myiasis producers in cairo and giza abattoirs.the most important insects from medical point of view, are the blood suckers or the insect-borne diseases. still others, almost non-blood suckers, but may attack man and animal to deposit their eggs or larvae causing pathogenic condition known as myiasis. in the present paper, a total of nine myiasis producing dipterous flies were collected from cairo and giza abattoirs. they were: musca d. vicina, calliphora vicina, chrysomyia albiceps, c. marginalis, lucilia sericata, l. cuprina, sarcophaga ha ...19911875078
impact of dung from ivermectin-treated sheep on oogenesis and survival of adult lucilia cuprina.females of the australian sheep blowfly, lucilia cuprina, exploit sheep dung as a source of protein for egg development. flies fed on dung collected from sheep 1 to 6 days after being drenched with the broad-spectrum antiparasitic drug ivermectin showed delayed reproductive development and reduced fecundity. male and female survival was also reduced. despite these effects, except where the recommended frequency of drenching (1 to 4 times per annum) is greatly exceeded, it is unlikely that iverme ...19911883296
[botulism in birds living in an aquatic environment in nové mlýny in the breclav district].epizootic of wild water birds caused by the toxin clostridium botulinum of c type occurred in extensive shallow artificial lakes of the nové mlýny dam system from may to october 1988 and later on from february to early april 1989. in total more than 3,000 dead or dying birds of 44 species were found, the following orders were prevailing: anseriformes (56%), lariformes (33%), charadriiformes (6%) and ralliformes (4%). botulotoxin was detected at high concentrations in sarcophagic fly larvae of ca ...19911926683
cloning and characterization of the white and topaz eye color genes from the sheep blowfly lucilia cuprina.clones carrying the white and topaz eye color genes have been isolated from genomic dna libraries of the blowfly lucilia cuprina using cloned dna from the homologous white and scarlet genes, respectively, of drosophila melanogaster as probes. on the basis of hybridization studies using adjacent restriction fragments, homologous fragments were found to be colinear between the genes from the two species. the nucleotide sequence of a short region of the white gene of l. cuprina has been determined, ...19901971656
spatial restriction of light adaptation and mutation-induced inactivation in fly photoreceptors.the spatial spread within fly photoreceptors of 2 forms of desensitization by bright light have been investigated: the natural process of light adaptation in normal musca photoreceptors and a receptor-potential inactivation in the no-steady-state (nss) mutant of the sheep blowfly lucilia. the suction-electrode method used for recording from vertebrate rods was applied to fly ommatidia. a single ommatidium in vitro was partially sucked into a recording pipette. illumination of the portion of the ...19912010812
persistence of clostridium botulinum type c toxin in blow fly (calliphoridae) larvae as a possible cause of avian botulism in spring.diverse samples were examined at a site of water-bird mortality, caused by clostridium botulinum type c toxin in southern moravia (czechoslovakia). the toxin was detected in high concentrations in mute swan (cygnus olor) carcasses (less than or equal to 1 x 10(6) ld50/g) as well as in necrophagous larvae and pupae of the blow flies lucilia sericata and calliphora vomitoria (less than or equal to 1 x 10(5) ld50/g) collected from them. it was detected in lower concentrations (less than or equal to ...19912023331
association of exogenous dna with cattle and insect spermatozoa in vitro.spermatozoa isolated from domestic cattle (bos taurus), the australian sheep blowfly (lucilia cuprina), and the honeybee (apis mellifera) are capable of binding exogenous radiolabeled linear dna. both motile and nonmotile bovine sperm exhibit four distinct patterns of dna association. following treatment with dnase i, the relative proportion of one of these patterns increases specifically in living sperm, suggesting that a small proportion of dna that associates with bovine sperm may be sequeste ...19912054177
tryptic and chymotryptic proteases released by larvae of the blowfly, lucilia cuprina.proteases released by larvae of the sheep blowfly have been suggested to have a primary role in wound formation and larval nutrition. assays were carried out on two larval products to analyse the substrate specificity of these proteases, their abundance and approximate molecular weights. tryptic and chymotryptic activities were found in both products though there were more chymotrypsin-like enzymes in products from 48 h cultures (cesp) than in product collected direct from 48 h larvae (lesp). so ...19902074126
[lucilia sericata infestation of the skin openings for the bone traction device in lengthening of the tibia: apropos of a case].fly larvae are responsible for animal disease well known by veterinarians. however, their finding in humans in nontropical countries is a rarity. recently, we have found several larvae of lucilia sericata in an achondroplastic child during the phase of lengthening of both tibiae with osteotax. we think that the myasis diagnosed in our patient is the first such case reported in the literature. for the time being, it should be considered a fortuitous finding and not as a complication of surgery.19902081173
[the occurrence of diptera in living quarters].more than 150 species of diptera belonging to 46 families were caught in a flat in the outskirts of berlin between april and october 1986. 2148 specimens were collected. fannia canicularis was the most frequent species with 726 specimens. drosophila melanogaster, culex pipiens, lucilia sericata, sarcophaga carnaria, calliphora vicina, muscina stabulans and fannia manicata were other important synanthropic flies, which inclusively fannia canicularis amounted 55% of the total catching rate. the re ...19902127165
prospects for the control of sheep blowfly strike by vaccination.research into vaccination against flystrike is aimed at either controlling the predisposing condition, fleece rot, or direct control of the fly maggots. a vaccine against the major bacterial species found in fleece rot lesions, pseudomonas aeruginosa, is undergoing field trials and results suggest that this vaccine may reduce fleece rot incidence. problems to be investigated include the existence of variants of p. aeruginosa in the field and the involvement of other species of bacteria in fleece ...19902210946
nocturnal oviposition behavior of blow flies (diptera: calliphoridae).it is widely held that blow flies, in general, and phaenicia sericata (meigen), in particular, are not active at night and do not lay eggs during that time. p. sericata is thought to require sunlight and warmth for oviposition. three common and forensically important flies--calliphora vicina (robineau-desvoidy), p. sericata, and phormia regina (meigen)--oviposited during the dark hours of the night during the summers of 1988 and 1989. nocturnal oviposition can alter the usual estimate of the pos ...19902231617
susceptibility of mulesing wounds to oviposition and strike development by the australian sheep blowfly lucilia cuprina.the age and/or physical condition of mulesing wounds had a significant effect on both the oviposition response of l. cuprina and the ability of the wound to support a strike. up to 48 h after mulesing, untreated wounds elicited a strong oviposition response in contrast with chemically treated wounds, although subsequent larval development was negligible. seven to 9-day-old wounds, however, regardless of the wound treatment, were highly attractive oviposition sites, which subsequently developed i ...19902288537
studies on glycosphingolipids in larvae of the green-bottle fly, lucilia caesar: two neutral glycosphingolipids having large straight oligosaccharide chains with eight and nine sugars.two neutral glycosphingolipids having large straight oligosaccharide chains with eight and nine sugars, provisionally named cos and cns, were isolated and purified from larvae of the green-bottle fly, lucilia caesar, as the only two remaining unidentified significant neutral glycolipids in this organism. from the results of sugar analysis, permethylation, negative-ion fast atom bombardment mass spectroscopy (fab-ms), and 1h-nmr studies, the structures of the two glycolipids are proposed to be: c ...19902391351
characterization of proteolytic and collagenolytic enzymes from the larvae of lucilia cuprina, the sheep blowfly.isoelectric focusing was used to characterize proteolytic enzymes in homogenate and excretory-secretory preparations of the larvae of l. cuprina, the sheep blowfly. zymogram overlays showed that the larvae produce a number of highly active proteases which have a wide range of isoelectric points and molecular weights. the alkaline and neutral pi proteases were inhibited by phenylmethyl-sulfonylfluoride, leupeptin and aprotinin; this indicated the presence of serine in the active site. pepstatin a ...19882480778
the immune response of the sheep popliteal lymph node to a purified phenoloxidase from larval cuticle of the sheep ectoparasite, lucilia cuprina.the popliteal lymph node of the sheep can mount a strong immune response to a phenoloxidase, enzyme a, purified from larval cuticle of a major sheep ectoparasite, the sheep blowfly, lucilia cuprina. continuous sampling from a cannulated efferent lymphatic allowed monitoring of changes in both cellular output (total cells and large blast cells) and specific antibody production (by elisa) following primary and secondary challenge with antigen. anti-enzyme a antibodies in lymph selectively precipit ...19892493091
monitoring and selection of resistance to pyrethroids in the australian sheep blowfly, lucilia cuprina.field and laboratory populations of the australian sheep blowfly, lucilia cuprina (wiedemann) (calliphoridae), were surveyed by bioassay for possible resistance to the synthetic pyrethroids, a group of insecticides under development for blowfly control. a normal distribution of lc50 values was found using deltamethrin, the test pyrethroid, with no indication of specific resistance despite widespread use of deltamethrin on sheep to control the sheep body louse, damalinia ovis (schrank) (trichodec ...19892519674
occupational allergy in an entomological research centre. ii. identification of ige-binding proteins from developmental stages of the blowfly lucilia cuprina and other species of adult flies.sera from 30 workers in an entomological research institute and from five sydney asthmatics, all with serum ige antibodies to sheep blowfly (lucilia cuprina) antigens, were studied with the aim of identifying individual ige-binding proteins in extracts of l. cuprina adult flies, larvae and eggs, and in extracts of related species of flies from the order diptera. using protein blotting, 21, 18 and nine different ige-binding components were identified in extracts of l. cuprina adult flies, larvae ...19892758354
occupational allergy in an entomological research centre. i. clinical aspects of reactions to the sheep blowfly lucilia cuprina.twenty eight per cent (15) of 53 workers engaged in a sheep blowfly breeding programme designed to control genetically the pest lucilia cuprina experienced allergic manifestations resulting from contact with this insect. the most common symptoms were rhinitis, affected eyes, rashes, and lower respiratory symptoms, usually, but not always, immediate in type. a personal history of non-insect related asthma, allergic rhinitis, or eczema, or a combination of these was more common in the fly allergic ...19892765420
characterization of two glucuronic acid-containing glycosphingolipids in larvae of the green-bottle fly, lucilia caesar.two glucuronic acid-containing glycosphingolipids were purified from larvae of the green-bottle fly, lucilia caesar by deae-sephadex and iatrobeads column chromatography. structures of these acidic glycolipids, glycolipids x and y, were elucidated by means of sugar analysis, permethylation, enzymatic hydrolysis, negative-ion fast atom bombardment mass spectrometry, and nmr studies. glycolipid x was determined to have the following structure: glca beta 1-3gal beta 1-3galnac alpha 1-4 galnac beta ...19892768250
experimental reconstruction of calliphora erythrocephala and lucilia sericata life-cycles in the growth cabinet. 19882979436
the larvae of some blowflies of medical and veterinary importance.diagnostic features are described as a series of couplets that enable separation of the third instar larvae of the following pairs of closely related forms of blowflies of medical and veterinary importance: chrysomya chloropyga (wiedemann) and ch.putoria (wiedemann), chrysomya albiceps (wiedemann) and ch.rufifacies (macquart), cochliomyia hominivorax (coquerel) and co.macellaria (fabricius), lucilia sericata (mergen) and l. cuprina (wiedemann), calliphora augur (fabricius) and c. stygia (fabrici ...19872979525
simulation of fly-waves to assess the ability of diflubenzuron to protect sheep against flystrike by lucilia cuprina.sheep were exposed to mass-released gravid females of the sheep blowfly, lucilia cuprina, in a fly-proof animal house at various times after treatment with the insecticide diflubenzuron (1000, 1500 and 2500 ppm a.i.). untreated sheep were similarly exposed as controls, while sheep treated with either diazinon (400 ppm a.i.) or cyromazine (1000 ppm) were used as standards for comparison. before exposure, groups of sheep were wetted by simulating rainfall in the animal house in order to increase t ...19873113043
ultrastructural localization of phenoloxidases in cuticle and haemopoietic tissue of the blowfly lucilia cuprina.the ultrastructural localization of two types of biochemically characterized phenol oxidase activity is described in the larva of the sheep blowfly, lucilia cuprina. cuticular tyrosinase activity (enzyme a) is seen in epicuticular filaments and procuticle. procuticle activity can be detected only after a presumed process of activation takes place in damaged cuticle. by using either the dopamine reaction or inducing melanization by hot-water treatment, tyrosinase is readily shown in haemopoietic ...19883148210
light reduces the excitation efficiency in the nss mutant of the sheep blowfly lucilia.the nss (no steady state) phototransduction mutant of the sheep blowfly lucilia was studied electrophysiologically using intracellular recordings. the effects of the nss mutation on the receptor potential are manifested in the following features of the light response. (a) the responses to a flash or to dim lights are close to normal, but the receptor potential decays close to the baseline level during prolonged illumination after a critical level of light intensity is reached. (b) the decline of ...19883225552
genetic architecture and adaptation: quantitative analysis of sheep and refuse tip populations of the australian sheep blowfly, lucilia cuprina.phenotypic differentiation between geographic areas and between sheep and adjacent refuse tip populations was assessed by quantitative analysis of population samples of l. cuprina from new south wales (lismore) and victoria (mansfield). in addition the genetic structure of populations has been defined and compared by biometrical analysis techniques. for all morphological and fitness characters examined significant phenotypic differentiation was observed both between geographic localities and bet ...19873274653
rearing phaenicia sericata (diptera: calliphoridae) on dry cat food with csma. 19883392716
a method of measuring the oviposition rate of the sheep blowfly, lucilia cuprina under field conditions.a simple technique, which allows sheep to be rendered temporarily susceptible to fly attack, is described and used to examine oviposition rates of lucilia cuprina under field conditions. oviposition rates are shown to be related to both fly activity and fly density. the question of hobson's 'live sheep factor' in attracting l. cuprina to sheep is re-considered.19883401147
[human case of disseminated cutaneous myiasis due to phaenicia sericata]. 19863620081
the humoral immune response of sheep to antigens from larvae of the sheep blowfly (lucilia cuprina). 19873654049
heterorhabditis spp. and steinernema (= neoaplectana) spp.: temperature, and aspects of behavior and infectivity.the infectivity of several species/strains of nematodes of the genera heterorhabditis and steinernema for postfeeding, 3rd instar larvae of the sheep blowfly, lucilia cuprina, was tested in sand at various temperatures. of the two genera, the steinernematids were more active at lower temperatures and parasitized l. cuprina over a greater temperature range. the temperature range of infectivity for l. cuprina differed between nematodes of the same genus and between strains of the same species. par ...19863743713
maternal and larval effects of photoperiod on the induction of larval diapause in two species of fly, calliphora vicina and lucilia sericata.the induction of larval diapause in the blowflies calliphora vicina and lucilia sericata was studied in relation to maternal and/or larval exposure to photoperiod. in c. vicina, maternal experience of short daylength was shown to be more important than larval exposure, although diapause was only observed when the larval rearing temperature was below 15 degrees c. the critical daylength was between 12 and 15 h per day. recovery from diapause was temperature- but not photoperiod-dependent. some ev ...19863817113
myiasis in a turkey.a case of cutaneous myiasis was reported in a 3.5-month-old turkey. the bird was invaded by a very large number of maggots on its posterior half. the maggots killed the bird and consumed all the flesh within 48 hours. microscopic examination of the maggots revealed that they belonged to lucilia sericata (a dipterous fly). the incidence of myiasis in birds was also discussed.19853833223
ultrastructural changes in the cuticle of the sheep blowfly, lucilia, induced by certain insecticides and biological inhibitors.the ultrastructural effects on larval cuticle of lucilia cuprina of two inhibitors of chitin synthesis, diflubenzuron and polyoxin d and an inhibitor of dihydrofolate reductase, aminopterin, are compared with those of the insecticide, cyromazine. diflubenzuron and polyoxin d both prevent the formation of a normal lamellate appearance in procuticle and interfere with deposition of epicuticle. aminopterin and cyromazine cause necrotic lesions in the cuticle which, in the case of cyromazine, are co ...19853923653
ecological analysis of field trials conducted to assess the potential of sex-linked translocation strains for genetic control of the australian sheep blowfly, lucilia cuprina (wiedemann).field trials were conducted with translocation/eye colour (te) strains of l. cuprina to measure the mating ability of the males under field conditions and assess their potential for suppressing sheep blowfly populations. rates of increase in l. cuprina were highest in spring (3.9-9.1 per generation), consistently low during summer (0.1-0.6 per generation) and somewhat higher during autumn (1.1-3.4 per generation). the te strains released had the potential to prevent population increases of this ...19854091754
genetic analysis of field trials of sex-linked translocation strains for genetic control of the australian sheep blowfly lucilia cuprina (wiedemann).the results of progeny tests of males and females captured during two field trials of sex-linked translocation strains for genetic control of l. cuprina are presented. males released as mature larvae survived to adulthood and mated with field females. however, the levels of genetic death introduced into the population were insufficient to suppress the native population. this was due partly to seasonal ineffectiveness of the release method, and partly to poor performance of the released males. on ...19854091755
aspects of the development of flight-muscle sarcosomes in the sheep blowfly, lucilia cuprina, in relation to changes in the distribution of protein and some respiratory enzymes during metamorphosis.1. changes in the amounts and distribution of protein and respiratory enzymes have been estimated during the life cycle of the fly lucilia cuprina. 2. the fully fed larva contains about 7mg. of protein, the pupa and newly emerged fly about 4mg., and the mature adult about 3mg. 3. there are two periods of incorporation of protein into particles at the expense of the soluble protein; the first, immediately after pupation, may store protein (0.5mg./insect) for use in adult development; the second, ...19674291561
[myiasis of the skin in the goose and dog due to the gold fly species lucilia sericata (meigen 1826)]. 19724559089
[picture report 13. myiasis in sheep (caused by lucilia sericata)]. 19734582070
relationship between diapause and spercooling in the blowfly, lucilia sericata (mg.) (diptera: calliphoridae). 19724656827
prasinons a and b: potent insecticides from streptomyces prasinus.two metabolites which have high activity against sheep blowfly larvae (lucilia sericata and l. cuprina) were found to be produced by streptomyces prasinus ncib 10719. these substances were isolated from culture filtrate by solvent extraction and chromatography and named prasinons a and b. fermentation factors affecting the formation of these substances are described together with their physical, chemical, and biological properties.19734762390
changes in dry weight, protein, and nucleic acid content during diapause and normal development of the blowfly, lucilia sericata. 19734773929
carbamate resistance in the sheep blowfly, lucilia cuprina (wied). 19734782514
evaluation of the potential use for several organotin compounds against the sheep blowfly (lucilia spp.). 19725014719
a method of measuring the susceptibility to insecticides of larvae of the sheep blowfly, lucilia spp. 19715095944
the metabolism of 3,5-di-tert.-butylphenyl n-methylcarbamate in insects and by mouse liver enzymes.the oxidation of 3,5-di-tert.-butylphenyl n-methylcarbamate (butacarb) has been studied in the flies musca domestica and lucilia sericata, grass grubs costelytra zealandica and the mouse. in all species eleven oxidation products, which were formed by hydroxylation of the tert.-butyl groups and the n-methyl group, were detected.19715144737
new insecticides: as sheep dips against lucilia sericata. 19715283469
pheromonal stimulation of the sexual activity of males of the sheep blowfly lucilia cuprina (calliphoridae) by the female. 19695370967
cuticular lipids of adults and puparia of the australian sheep blowfly lucilia cuprina (wied.).the presence of a strong contact component in the sex and ovipositing behavior of the sheep blowfly lucilia cuprina wied. prompted an investigation into the chemical composition of the cuticular wax of the adult male and female flies as well as that of the blowfly puparia. thin-layer chromatography indicated that the lipids in all the waxes examined comprise hydrocarbons, nonglyceryl esters, triglycerides, free fatty acids, and hydroxy compounds, probably diglycerides and monoglycerides. phospho ...19705411981
distribution of long-chain fatty acids among the lipid classes of lucilia sericata (meigen) and galleria mellonella (linnaeus). 19705475086
olfactory unit potentials and receptor potential responses of lucilia sericata. 19715574668
[human myiasis caused by phaenicia sericata]. 19675598428
[human myiasis caused by phaenicia sericata (meigen, 1826) in chile (diptera, calliphoridae)]. 19675603109
a resistance mechanism in organophosphorus-resistant strains of sheep blowfly (lucilia cuprina). 19685643263
isolation of sphingoethanolamine from pupae of the green-bottle fly, lucilia caesar. 19685707810
polytene chromosomes and linkage group-chromosome correlations in the australian sheep blowfly lucilia cuprina (diptera: calliphoridae). 19695800665
the chemosterilisation of the sheep blowfly, lucilia sericata (meig.) with apholate. 19655889842
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