Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| role of monoamine metabolism in wing dimorphism of the aphid myzus persicae. | 1976 | 11073 | |
| [breeding of a biotype of the aphia myzus persicae on a synthetic medium. iv. influence of vitamins, especially vitamin c and pyridoxine, on the nutritional value of nutritive fluids]. | the nutritive value of synthetic diets for myzus persicae was evaluated by measuring reproduction of the third generation on each medium: 1. in the presence of a double dose of pyridoxin, the nutritional value of the basic diet was improved. this improvement disappeared when all the vitamins of the b group were also at double dose; 2. in the presence of a double dose of ascorbic acid, the nutritional value of all the diets was improved. this improvement was observed for a single or a double dose ... | 1978 | 104648 |
| acetaldehyde--a potential fumigant for control of the green peach aphid on harvested head lettuce. | 1979 | 528743 | |
| [laboratory and field tests about the transmission of erysimum latent virus by phyllotreata species (chrsomelidae) (author's transl)]. | erysimum latent virus (elv) proved to be transmissible by phyllotreta atra, p. nemorum, p. nigripes, and p. undulata. from them p. nemorum is a new discovered vector. the acquisition of the virus by the beetles was possible within a feeding time of less than 10 minutes. because virus transmission could be performed within 30 to 60 minutes immediately after virus uptake there exists apparently no latent period in the vector. the retention or persistence of elv in the phyllotreta species amounted ... | 1977 | 565119 |
| the properties of a carboxylesterase from the peach-potato aphid, myzus persicae (sulz.), and its role in conferring insecticide resistance. | carboxylesterases from different strains of myzus persicae were examined to try to understand their contribution to insecticide resistance. preliminary evidence that they are involved comes from the good correlation between the degree of resistance and the carboxylesterase and paraoxon-degrading activity in aphid homogenates. furthermore the carboxylesterase associated with resistance could not be separated from the insecticide-degrading enzyme by electrophoresis or ion-exchange chromatography. ... | 1977 | 603629 |
| [a conbribution to the characterization of asparagus virus 2 (author's transl)]. | in host range investigations with 152 plant species of 37 families, 96 species from 28 families proved to be experimentally susceptible to asparagus virus 2 (asv 2). from these, 82 species of 26 families were systematically infectible and 14 species of 10 families only locally. most frequently were symptomless systemic infections. of 86 species, their susceptibility for asv 2 was unknown before. the number of families that contant experimental hosts of the virus increased from 11 to 33. transmis ... | 1978 | 664937 |
| electrophoretic study of genetic variation in british myzus persicae (sulz.) (hemiptera, aphididae). | 1978 | 743199 | |
| simultaneous transmission of broad bean wilt and cucumber mosaic viruses by single myzus persicae (sulz.). | 1976 | 947110 | |
| [rearing of a biotype of myzus persicae sulzer on synthetic media. iii. respective nutritive value of l-methionine, l-cysteine and sulfates]. | the respective influence of some different components of a synthetic diet on the survivial and the fecundity of one strain of myzus persicae slz. have been established by experiments with the deletion of some components and with variations of their concentrations. 1. l.methionin is the only essential amino-acid amd the variations of its concentrations are related with variations of the fecondity of the aphids. 2. when the amount of l. methionin is inadequate to satisfy the whole sulphur requirem ... | 1975 | 1227369 |
| engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus x and potato virus y in transgenic russet burbank. | potato virus x (pvx) and potato virus y (pvy) infection in potato may result in the loss of certification of seed potatoes and affect quality and yield of potatoes in commercial production. we transformed a major commercial cultivar of potato, russet burbank, with the coat protein genes of pvx and pvy. transgenic plants that expressed both cp genes were resistant to infection by pvx and pvy by mechanical inoculation. one line was also resistant when pvy was inoculated with viruliferous green pea ... | 1990 | 1366358 |
| resistance to phloem transport of potato leafroll virus in potato plants. | a 'double-graft sandwich' technique in which sections of potato stem from different potato cultivars were grafted between a susceptible healthy stock plant and a potato leafroll virus (plrv)-infected scion was used to study the rate of phloem transport of plrv in cultivars differing in resistance to plrv infection (ir) and accumulation (ar). resistance to phloem transport (i.e. delayed plrv systemic movement) was found in bismark cultivar (ir a(s)). this was independent of ir and ar as the rate ... | 1992 | 1469360 |
| insect-mediated transmission of mixed and reassorted cucumovirus genomic rnas. | transmissions of virus using the aphid myzus persicae were performed using plants co-infected with two cucumoviruses, tomato aspermy virus (v-tav) and cucumber mosaic virus (m-cmv). five of the aphid-transmitted progeny viruses (3.7%) induced symptoms distinct from those induced by either parental virus. northern blot hybridization analysis of encapsidated rnas from these novel progeny demonstrated that all of the rna profiles were characteristic of pseudo-recombinants, i.e. viruses with reassor ... | 1992 | 1645147 |
| visualization of a luteovirus in the vector aphid's body by two gold immunolabelling techniques: a comparative study. | two gold immunolabelling techniques using electron microscopy were compared to examine the in situ localization of a luteovirus, potato leafroll virus (plrv), inside its main aphid vector, myzus persicae sulz. with gildow's technique, virus particles were labelled prior to fixation, embedding by injecting plrv-specific iggs into the living aphids. this facilitated the detection of extracellular particles located between the basal lamina and plasmalemma by trapping them in aggregates. the heavy c ... | 1991 | 1800527 |
| comparison of short- and long-feed transmission of the cauliflower mosaic virus cabb-s strain and s delta ii hybrid by two species of aphid: myzus persicae (sulzer) and brevicoryne brassicae (l.). | the cauliflower mosaic virus (camv) hybrid s delta ii, partially deleted in orfii, loses its transmissibility by the aphid myzus persicae on 5-min acquisition feed. we have also shown that it is not transmitted after 8-h acquisition feed. the same occurs with brevicoryne brassicae. therefore, the aphid transmission factor (atf) is involved in both means of transmission and in both aphid species. m. persicae can acquire camv cabb-s strain in less than 20 s. m. persicae is a more efficient vector ... | 2006 | 2087600 |
| [a comparative study of the effect of vinyl phosphoric acid esters on cholinesterase and carboxylesterase activities in mammals and arthropods]. | studies have been made of the effect of organophosphorus inhibitors on cholinesterase and carboxylesterase from various mammals (human erythrocytes, mouse brain, blood serum of mouse and rat, blood serum of horse) and arthropods (calliphora vicina, schizaphis graminum, myzus persicae, sitophilus oryzae, pseudococcus maritimus, tetranychus urticae). organophosphorus inhibitors were presented by esters of vynylphosphoric acid containing normal and branched alkyls in the phosphoryl part of the mole ... | 1990 | 2360379 |
| coat protein of potyviruses. 4. comparison of biological properties, serological relationships, and coat protein amino acid sequences of four strains of potato virus y. | four strains of potato virus y, pvy-d, pvy-10, pvy-18, and pvy-43, obtained from different australian sources were compared on the basis of their biological, serological and coat protein structural properties. each of the strains could be distinguished on the basis of their reactions on selected test plant species. two of the pvy strains, pvy-d and pvy-10, induced symptoms similar to those produced by the pvyo strain group. the reactions of pvy-18 and pvy-43, although comparable to pvyn in some ... | 1988 | 3144263 |
| molecular evidence that insecticide resistance in peach-potato aphids (myzus persicae sulz.) results from amplification of an esterase gene. | cdna clones for the esterase (e4) responsible for broad insecticide resistance in peach-potato aphids (myzus persicae sulz.) were isolated and used to study the molecular basis of resistance. increased esterase synthesis by resistant aphids was found to be associated with amplification of the structural gene for the esterase (e4 or its closely related variant, fe4), the degree of amplification being correlated with the activity of the esterase and the level of resistance. hybridization of the cd ... | 1988 | 3390158 |
| [salivary glands of myzus persicae (homoptera, aphididae): structure and secretory rhythm]. | the salivary glands of myzus persicae (homoptera, aphididae): structure and secretory rythm. studying the structure of the salivary glands of myzus persicae, a particular mode of working of these organs were observed. the glands seem to function in an alternative manner. this aspect is discussed with regards to the nutritional biting of the bug. | 1987 | 3442441 |
| a multiple homogenizer for rapid sample preparation in immunoassays and electrophoresis. | a multiple homogenizer is described for preparing samples of small invertebrates or tissue in a flat-bottom immunoplate. its efficiency was evaluated by immunoassay of carboxylesterase (e4), the enzyme conferring insecticide resistance in the peach potato aphid (myzus persicae). this equipment was shown to release more enzyme, with less variability, than homogenizing individual aphids and its efficiency allows one person to analyze up to 3000 individual insects per day. it is also suitable for p ... | 1987 | 3447586 |
| overproduction of detoxifying esterases in organophosphate-resistant culex mosquitoes and their presence in other insects. | antisera raised against the denatured polypeptide of two organophosphate-detoxifying esterases (b1 and a1) of culex mosquitoes were used in an immunoblot method to quantify esterase production in resistant versus susceptible strains and to detect the presence of immunologically related proteins in other insects. it was demonstrated that esterase b1 of culex quinquefasciatus and esterase a1 of culex pipiens are overproduced in resistant strains by factors of at least 500-fold and 70-fold, respect ... | 1987 | 3470782 |
| preference of the green peach aphid, myzus persicae, for plants grown in sewage sludges. | 1987 | 3663979 | |
| an electrophoretic study of some hydrolases from an op-susceptible and an op-resistant strain of the green peach aphid, myzus persicae (sulz.). | 1973 | 4350965 | |
| [ultrastructural aspects of the salivary glands in the aphid myzus persicae (suiz.) homoptera aphididae]. | 2015 | 4463419 | |
| incorporation of thymidine into the chromosomes of aphid (myzus persicae) embryos. | 1974 | 4474087 | |
| [the effect of salivary gland (obtained from myzus persicae sulzer) extract on broad bean epidermal cells infected with bean yellow mosaic virus (author's transl)]. | 1974 | 4478240 | |
| [investigations of beet mild yellowing. 1. virus transmission by myzus persicae sulz. after feeding of plant extracts through an artificial membrane (author's transl)]. | 1974 | 4480507 | |
| control of green peach aphid on sweet peppers in delaware. | 1972 | 4628649 | |
| control of the green peach aphid and suppression of leaf roll on potatoes by systemic soil insecticides and multiple foliar sprays. | 1973 | 4690263 | |
| an in vitro study of esterases, hydrolysing non-specific substrates, of an op-resistant strain of the green peach aphid, myzus persicae (sulz.). | 2016 | 4714903 | |
| an in vitro study of esterases hydrolysing specific substrates of an op-susceptible and an op-resistant strain of the green peach aphid, myzus persicae sulz. | 1973 | 4788463 | |
| [effect of atp in a synthetic diet on myzus persicae (sulz.) (aphidina)]. | 1974 | 4847667 | |
| the fine structure of the mycetome symbiotes of the aphids brevicoryne brassicae, myzus persicae, and macrosiphum rosae. | 1971 | 4999588 | |
| an insecticide and a defoliant evaluated for use in a program of integrated control designed to suppress the green peach aphid on peach trees. | 1972 | 5008977 | |
| transmission of tobacco mosaic virus by myzus persicae. | 1972 | 5084452 | |
| insect predators for controlling aphids on potatoes. 8. green peach aphid consumption by coccinella septempunctata and c. transversoguttata. | 1972 | 5085802 | |
| effects on myzus persicae (sulz.) and transmission of beet yellows virus of applying certain trace elements to sugar beet. | 1971 | 5133524 | |
| effect of artificial diet composition on wing-production by the aphid myzus persicae. | 1970 | 5449726 | |
| comparison of the biology of myzus persicae sulz. resistant and susceptible to dimethoate. | 1970 | 5491865 | |
| chromosomal abnormalities in an anholocyclic biotype of myzus persicae (sulzer). | 1971 | 5556464 | |
| mass rearing parasite-free green peach aphids in the greenhouse. | 1971 | 5558295 | |
| dietary amino acid requirements of the aphid myzus persicae affected by antibiotic uptake. | 1971 | 5565091 | |
| [effect of some factors on the development of alate forms in the peach louse (myzus persicae sulz.)]. | 1967 | 5586737 | |
| aphid transmission of tobacco mosaic virus. | aphids (myzus persicae sulz.) can acquire tobacco mosaic virus from tobacco leaves coated with a virus suspension and inoculate it into healthy leaves. transmission depends on virus concentration, period of acquisition, previous feeding history of the aphids, and time between acquisition and transmission feedings. aphids whose stylets are cut do not transmit the virus. | 1969 | 5783713 |
| studies on the artificial feeding of the aphid myzus persicae (sulzer)--3. some major nutritional requirements. | 1965 | 5827534 | |
| [some relations between the common pea mosaic virus and the peach tree aphis myzus persicae sulz]. | 1965 | 5856645 | |
| endosulfan, oxydemetonmethyl, and endrin in control of the green peach aphid and suppression of leaf roll in potatoes in eastern washington. | 1966 | 5976106 | |
| the purification of potato leafroll virus from its vector myzus persicae. | 1967 | 6017406 | |
| improvement of synthetic diet for the aphid myzus persicae using plant juices, nucleic acids, or trace metals. | 1967 | 6046968 | |
| feeding behaviour and susceptibility of myzus persicae (sulz) and brevicoryne brassicae (l.) to [14c] phorate in artificial diet. | the effect of artificial diet on the growth and reproduction of the aphids myzus persicae (sulz) and brevicoryne brassicae (l) was studied. following the application of [14c] phorate to the diet, the results showed that b. brassicae was more susceptible than m. persicae. in these experiments the dead and live aphids and their honeydew were also analysed. the dead aphids contained more toxic 14c than the live ones with a direct correlation between the amount of insecticide in the diet and the ins ... | 1980 | 6155883 |
| toxicity of [14c] phorate applied systemically to the aphids myzus persicae (sulz) and brevicoryne brassicae (l.) grown on brussels sprout plants. | the effect of foliar application of [14c] phorate on the larvae of m. persicae and b. brassicae was studied. regression analysis of the toxic components in the foliage and the probit kill showed that b. brassicae was more susceptible to the insecticide than m. persicae. the level of radioactivity extracted from the leaves, aphids and their honeydew shows that there was no correlation between the applied dose and the mortality. the amounts of honeydew produced by colonies before and after applica ... | 1980 | 6159846 |
| maximizing the detection capability of a beet western yellows virus elisa system. | conditions for maximizing detection of a california isolate of beet western yellows virus (bwyv) were investigated with the double-sandwich enzyme-linked immunosorbent assay (elisa) system. within-plate variability was found to account for less than 1% of the total variation observed on individual microtiter plates. variability across plates was greater than within plates and accounted for less than 10% of the total variation. significant reductions in absorbance were observed when either coatin ... | 1984 | 6392314 |
| disappearance of dimethoate, methamidophos and pirimicarb in lettuce. | foliar sprays of dimethoate at 150 or 300 g a.i./ha, methamidophos at 450 or 900 g a.i./ha and pirimicarb at 140 or 280 g a.i./ha were applied for control of the green peach aphid, myzus pericae (sulzer), and the lettuce aphid, nasonovia ribisnigri ( mosley ), about 2 weeks before the lettuce started heading, and again about 1 week from harvest. in lettuce, dimethoate partially oxidized to its oxon and pirimicarb converted to its methylamino- and/or formyl methylamino-analogues. most residues we ... | 1984 | 6736564 |
| [breeding of a biotype of myzus persicae sulzer on a synthetic medium. v- influence of oxalic and gentisic acids on the nutritive value of a synthetic medium]. | studies on the survival, fecundity, and food consumption by m. persicae reared on synthetic diets, have indicated: --gentisic and oxalic acids have a deleterious effect as most important as their dose is high. --succinic acid reduces, at least for the used concentrations, the deleterious effect of the oxalic acid, but not that of the gentisic acid. --gentisic acid is quickly decreasing food consumption. --the deleterious effect of oxalic and gentisic acids is not decreasing with time. the result ... | 1980 | 7416660 |
| changes in the amino acid sequence of the coat protein readthrough domain of potato leafroll luteovirus affect the formation of an epitope and aphid transmission. | potato leafroll luteovirus (plrv) is transmitted naturally by aphids, but two isolates (15 and v) are known to be only poorly transmissible (pat); these isolates are also distinct in that their particles lack an epitope present in transmissible (hat) isolates of plrv (tamada et al., ann. appl. biol. 104, 107-116, 1984; massalski and harrison, j. gen. virol. 68, 1813-1821, 1987). virus cultures propagated vegetatively for several years in potato plants from the source of isolate v were shown stil ... | 1994 | 7513925 |
| transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus y helper component. | two spanish plum pox virus (ppv) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector myzus persicae, with woody and herbaceous host plants. these isolates differ in the size of their coat protein (cp) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the n terminus of the cp, affecting the same positions as in a previously reported non-aphid-transmissible ppv isolate from germany. aphid transmission experiments showed that isol ... | 1995 | 7561767 |
| [a preliminary study on bioactivity of orange and tangerine peel extracts against aphis and mites]. | an assay was made on the bioactivity of the extracts of tangerine peel from cinocitrus tangerina, orange peel from citrus sinensis and mixed tangerine peel from cinocitrus sp. against aphis semia phis heraclei, aphis craccivora, uroleucon gobonis and myzus persicae using residual film or topical method, and against mites tetranychus viennensis and t. trancatus using slide-dip or immersion method. test results show that these extracts have strong bioactivity against aphids and mites. the correcte ... | 1995 | 7576134 |
| aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein p74. | beet western yellows luteovirus is obligately transmitted by the aphid myzus persicae in a circulative, non-propagative fashion. virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. virions contain two types of protein; the major 22 kda capsid protein and the minor read-through protein, p74, which is composed of the major capsid protein fuse ... | 1995 | 7882968 |
| the peach-potato aphid myzus persicae and the tobacco aphid myzus nicotianae have the same esterase-based mechanisms of insecticide resistance. | biochemical and molecular studies have established that in the peach-potato aphid, myzus persicae, insecticide resistance is conferred by amplification of genes encoding the insecticide-detoxifying esterases e4 or fe4. here we report that two insecticide-resistant clones of the closely related tobacco aphid myzus nicotianae have elevated esterases indistinguishable from e4 and fe4 and amplified esterase dna sequences, and flanking regions, with identical restriction maps to the m. persicae genes ... | 1994 | 7894746 |
| endosymbiotic bacteria associated with circulative transmission of potato leafroll virus by myzus persicae. | in order to understand the molecular mechanisms underlying circulative transmission of potato leafroll virus (plrv) by aphids, we screened myzus persicae proteins as putative plrv binding molecules using a virus overlay assay of protein blots. in this way, we found that purified plrv particles exhibited affinity for five aphid proteins. the one most readily detected has an m(r) of 63k, and was identified as symbionin. this is the predominant protein synthesized by the bacterial endosymbiont of t ... | 1994 | 7931143 |
| cytokinin-mediated insect resistance in nicotiana plants transformed with the ipt gene. | the bacterial isopentenyl transferase (ipt) gene involved in cytokinin biosynthesis was fused with a promoter from the proteinase inhibitor ii (pi-iik) gene and introduced into nicotiana plumbaginifolia. transcripts of the ipt gene were wound-inducible in leaves of transgenic pi-ii-ipt plants. in leaf disks excised from fully expanded leaves, transcript levels increased 25- to 35-fold within 24 h and by 48 h were reduced by about 50%. in flowering plants, message levels were 2- to 5-fold higher ... | 1993 | 8219068 |
| signal for potyvirus-dependent aphid transmission of potato aucuba mosaic virus and the effect of its transfer to potato virus x. | a british isolate of potato aucuba mosaic potexvirus (pamv) was transmitted by aphids (myzus persicae) which had fed previously on a source of potato y potyvirus (pvy). nucleotide sequence analysis of the pamv coat protein gene indicated that amino acid residues 14 to 16 from the n terminus of the coat protein have the sequence dag, which is also found in the coat proteins of potyviruses and is required for their aphid transmissibility. a recombinant virus isolate (txpa7) was produced in which a ... | 1993 | 8336116 |
| the intestine is a site of passage for potato leafroll virus from the gut lumen into the haemocoel in the aphid vector, myzus persicae sulz. | four detection techniques, three of which gave reliable identification of the virus particles, were used to locate potato leafroll virus (plrv) in the alimentary canal of its main aphid vector, myzus persicae sulz: immunofluorescence on cryostat sections, conventional transmission electron microscopy on ultrathin sections and immune electron microscopy with gold labeling, either prior to or after fixation-embedding. each method clearly showed the presence of the virus in the intestine epithelium ... | 1993 | 8347080 |
| cloning and analysis of the esterase genes conferring insecticide resistance in the peach-potato aphid, myzus persicae (sulzer). | full-length cdna clones encoding the esterases (e4 and fe4) that confer insecticide resistance in the peach-potato aphid [myzus persicae (sulzer)] were isolated and characterized. the e4 cdna contained an open reading frame of 1656 nucleotides, coding for a protein of 552 amino acids. the fe4 cdna shared 99% identity with e4 over this region, the most important difference being a single nucleotide substitution resulting in the fe4 mrna having an extra 36 nucleotides at the 3' end. the derived am ... | 1993 | 8373371 |
| synthesis of a full-length infectious cdna clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses. | a full-length cdna of cucurbit aphid-borne yellows virus (cabyv) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage t7 rna polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35s promoter in an agroinfection vector. the biological activity of the cloned cdna was demonstrated by the ability of its in vitro transcript to replicate in protoplasts and of the agroinfection vector to infect agroinoculated plants. virus in th ... | 1995 | 8525610 |
| analysis of amplicons containing the esterase genes responsible for insecticide resistance in the peach-potato aphid myzus persicae (sulzer). | the amplification of genes encoding an insecticide-detoxifying esterase (e4) in the peach-potato aphid myzus persicae is one of the few examples where this genetic phenomenon has been shown to be involved in the response of an intact higher organism to artificial selection. here we report quantitative and qualitative studies of the repeat units (amplicons) containing the e4 genes in a highly resistant aphid clone. initial studies to quantify esterase sequences showed a 5-11-fold increase in resi ... | 1996 | 8573090 |
| detection of potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potential epidemiological application. | a reverse transcription and polymerase chain reaction (rt-pcr) system was developed using two 20-mer primers located in the potato leafroll virus (plrv) capsid gene. a 336-bp pcr product was detected from aphids (myzus persicae) which had been fed on plrv-infected plants. the pcr band was specific to plrv as determined by southern blots and detection by a plrv-specific probe. as little as 5 min exposure of aphids to plrv-infected leaves resulted in the presence of plrv-specific bands in 13% of a ... | 1995 | 8576303 |
| changes in the methylation of amplified esterase dna during loss and reselection of insecticide resistance in peach-potato aphids, myzus persicae. | insecticide resistance in peach-potato aphids, myzus persicae, results from the amplification of genes encoding an esterase that hydrolyses and sequesters insecticides. resistance is normally stable, but highly resistant aphid clones sometimes lose resistance when insecticidal selection pressure is removed. this loss of resistance, termed reversion, arises from a loss of elevated esterase enzyme through transcriptional control, i.e. without loss of the amplified esterase dna sequences. we have s ... | 1996 | 8673078 |
| biological and molecular properties of a pathotype p-1 and a pathotype p-4 isolate of pea seed-borne mosaic virus. | two isolates of pea seed-borne mosaic potyvirus, dpd1 and ny, were identified as pathotypes p-1 and p-4, respectively, by their infectivity on pisum sativum l. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. the two isolates differed in several biological characteristics. dpd1 induced transient vein clearing, downward rolling of leaflets and internode shortening on p. sativum, whereas ny only caused a slight growth reduction. dpd1 moved systemically in chenopodium quinoa whe ... | 1996 | 8683223 |
| ultrastructural study of acquisition and retention of potato leafroll luteovirus in the alimentary canal of its aphid vector, myzus persicae sulz. | the chronology of plrv acquisition and retention by myzus persicae was investigated using electron microscopy. examination demonstrated a rapid translocation of the virus through the intestine into the haemocoel. indeed, viral particles could be observed in the intestinal epithelial cells, then in the haemocoel, 4 and 8 h, respectively, after their arrival in the lumen of the alimentary canal. however, the virus accumulated in the intestinal epithelial cells. in these cells, the first viral part ... | 1996 | 8774687 |
| detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. | a reverse transcription polymerase chain reaction (rt-pcr) assay was designed to amplify stylet-borne potato virus yo (pvyo) in aphids using primers located in the viral capsid gene. a 480 bp long product was detected in aphids exposed to pvyo-infected potato plants. approximately 40% of myzus persicae and 15% of aphis nasturtii exposed briefly to pvyo-infected plants acquired the virus. this rate of acquisition by both species of aphids was typical of our earlier observation of the virus transm ... | 1996 | 8793847 |
| polymerase chain reaction-based identification of insecticide resistance genes and dna methylation in the aphid myzus persicae (sulzer). | the ability of a peach-potato aphid (myzus persicae) to resist insecticides can depend on both the presence of amplified esterase genes and on differences in their expression. here we report a polymerase chain reaction-restriction enzyme (pcr-ren) technique which can detect the presence of amplified esterase genes and distinguish between the two possible types of amplified gene (e4 and fe4) and a homologous fragment from susceptible aphids. the technique is quick and sensitive enough to be used ... | 1996 | 8799738 |
| ribosomal dna is frequently concentrated on only one x chromosome in permanently apomictic aphids, but this does not inhibit male determination. | the ribosomal dna arrays in the nucleolar organizer regions (nors) of aphids are generally-located in a telomeric or subtelomeric position on the x chromosomes. in aphid populations or species that have lost the sexual part of their life cycle and become permanent apomicts, multiple rdna copies are often concentrated on only one of the original x chromosomes. this situation has been found in apomictic members of three aphid subfamilies, although not as yet in the tribe aphidini, which includes s ... | 1996 | 8817074 |
| potyvirus transmission is not increased by pre-acquisition fasting of aphids reared on artificial diet. | aphids (myzus persicae), fasted after removal from healthy rearing plants, transmitted tobacco etch potyvirus (tev) more efficiently than unfasted aphids whether virus acquisition was from infected leaves or through membranes. there was no difference in uptake of 125i-labelled tev by fasted or unfasted aphids as measured by liquid scintillation counting. when aphids acquired 125i-labelled tev, label was retained in the stylets (as determined by autoradiographic light microscopy) by 51 % of 272 f ... | 1996 | 9000109 |
| cdna cloning, baculovirus-expression and kinetic properties of the esterase, e3, involved in organophosphorus resistance in lucilia cuprina. | resistance to organophosphorus insecticides (ops) in the sheep blowfly, lucilia cuprina, is associated with a non-staining phenotype of the carboxylesterase isozyme, e3 (e.c. 3.1.1.1). here, we show that a member of alpha-esterase multigene family, lc alpha e7, encodes e3. an lc alpha e7 cdna has been isolated from an op-susceptible strain and expressed in a baculovirus. the expressed product is the same as e3 in its electrophoretic mobility and preference for alpha-over beta-naphthyl acetate as ... | 1997 | 9061925 |
| aphicide persistence on spinach and mustard greens. | laboratory bioassays with green peach aphids, myzus persicae (sulzer), were conducted on samples from field grown spinach and mustard greens to determine the persistence of triazamate, dimethoate, and mevinphos. treatment with each insecticide resulted in similar mortality initially on both crops. mortality on samples from mevinphos treated plants declined considerably by 1 d after treatment. dimethoate persisted for > 4 d at a level that would kill at least some aphids during the allotted time. ... | 1997 | 9071890 |
| structure and organization of amplicons containing the e4 esterase genes responsible for insecticide resistance in the aphid myzus persicae (sulzer). | insecticide resistance in the aphid myzus persicae results primarily from the amplification of genes encoding the insecticide-detoxifying esterase, e4. here we report the analysis of flanking dna co-amplified with the e4 gene. the 5' end of this gene has an untranslated leader sequence interspersed by two introns, and the promoter region lacks tata and caat boxes. the dna breakpoint involved in the generation of the amplification is just upstream (approx. 250 bp) of the putative e4 transcription ... | 1997 | 9148762 |
| evidence for heterologous encapsidation of potato spindle tuber viroid in particles of potato leafroll virus. | the aphid myzus persicae (sulz.) was shown to transmit potato spindle tuber viroid (pstvd) to potato clone dto-33 from source plants doubly infected with potato leafroll virus (plrv) and pstvd. transmission was of the persistent type and did not occur when the insects were allowed to feed on singly infected plants. only low levels of pstvd were associated with purified plrv virions, but its resistance to digestion with micrococcal nuclease indicates that the viroid rna is encapsidated within the ... | 1997 | 9191909 |
| potato leafroll virus binds to the equatorial domain of the aphid endosymbiotic groel homolog. | a groel homolog with a molecular mass of 60 kda, produced by the primary endosymbiotic bacterium (a buchnera sp.) of myzus persicae and released into the hemolymph, has previously been shown to be a key protein in the transmission of potato leafroll virus (plrv). like other luteoviruses and pea enation mosaic virus, plrv readily binds to extracellular buchnera groel, and in vivo interference in this interaction coincides with reduced capsid integrity and loss of infectivity. to gain more knowled ... | 1998 | 9420234 |
| evidence that the e4 and fe4 esterase genes responsible for insecticide resistance in the aphid myzus persicae (sulzer) are part of a gene family. | the amplification of genes encoding the esterases e4 and fe4 is a widespread mechanism of insecticide resistance in the peach-potato aphid, myzus persicae (sulzer). we present evidence that in susceptible aphids the two genes are adjacent to each other in a head-to-tail arrangement with e4 upstream of fe4 and with approx. 19 kb of intervening sequence. there are also at least two other closely related sequences which might come from other members of an esterase gene family, in line with reports ... | 1998 | 9461506 |
| amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the aphids myzus persicae and aphis gossypii. | cucumber mosaic virus (cmv) transmission by two aphid species was investigated using strains of the virus with modified coat proteins. chimeric viral rna 3 cdna constructs were designed with coat protein-encoding sequences from the efficiently aphid-transmitted fny-cmv and the very poorly aphid-transmitted m-cmv. different regions of the viral coat protein variably influenced transmission; the magnitude of effects depended on the aphid species used as vector. five amino acid changes in the coat ... | 1998 | 9501042 |
| a 169-base pair tandem repeat dna marker for subtelomeric heterochromatin and chromosomal rearrangements in aphids of the myzus persicae group. | numerous copies of a 169-base pair dna sequence (myzus persicae group repeat; mpr) occur at subtelomeric locations on all chromosomes of three members of the myzus persicae species group (myzus persicae, m. antirrhinii, m. certus). mpr occurs in large tandem arrays at both ends of all autosomes of the standard 2n = 12 karyotype, and near one end of the x chromosome (the end opposite to the nucleolar organizer) and is estimated to make up about 5% of the genome (a total of about 200000 copies). l ... | 1998 | 9609659 |
| orientation of the 'stretched' univalent x chromosome during the unequal first meiotic division in male aphids. | in situ hybridization was used to label the ends of the x chromosomes of two aphid species, myzus persicae and amphorophora tuberculata, in order to study the peculiar behaviour and orientation of the univalent x in aphid spermatogenesis. anaphase i begins with the long axis of the x chromosome at right angles to the spindle and its two chromatids closely associated, but as the division proceeds the chromatids separate along most of their lengths, retaining only a midway connection as the x chro ... | 1998 | 9609660 |
| role of the helper component in vector-specific transmission of potyviruses. | four aphid species were tested for their ability to transmit tobacco etch (tev) and turnip mosaic (tumv) potyviruses. myzus persicae and aphis gossypii transmitted both viruses efficiently from infected plants, whereas lipaphis erysimi transmitted only tumv and myzus ascalonicus was a poor or non-transmitter of either virus. similar electrically monitored probing patterns were produced by m. persicae, l. erysimi and m. ascalonicus, ruling out behavioural differences as the cause of differential ... | 1998 | 9634096 |
| cloning and functional characterisation of two novel nicotinic acetylcholine receptor alpha subunits from the insect pest myzus persicae. | nicotinic acetylcholine receptors play a major role in excitatory neurotransmission in insect cnss and constitute an important target for insecticides. here, we report the isolation and functional characterisation of two cdnas encoding nicotinic acetylcholine receptor alpha subunits from a major insect pest, the peach-potato aphid myzus persicae. these two subunits, termed mp alpha1 and mp alpha2, are respective structural homologues of the drosophila d alpha2/schistocerca gregaria alphal1 alpha ... | 1998 | 9721715 |
| a study of variation in rdna its regions shows that two haplotypes coexist within a single aphid genome. | we report variation in the rdna internal transcribed spacers (itss) of aphid species, the first for these insects. variation at 6 sites within its1 sequences of the green peach aphid, myzus persicae, identified two haplotypes coexisting within the same individuals, indicating that molecular drive has not homogenised different copies of rdna. during this study, we found that pcr can cause a precise 58-bp loss in the amplified copies of an its haplotype (type 1). this occurs in all detectable copi ... | 1998 | 9729768 |
| inhibition effect of transgenic tobacco plants expressing snowdrop lectin on the population development of myzus persicae. | the cdnas of snowdrop lectin mature protein and its precursor protein, gna12 and gna34, were inserted downstream of a 35s promoter with a double enhancer and a "omega" fragment of tmv-rna cdna in the binary vector pbin438, or the phloem-specific coymv promoter in the vector pbcop1, respectively, resulting in the construction of four plant expression vectors pbgna12, pbgna34, pbcgna12, and pbcgna34. leaf stripes of nicotiana tabacum var. k326 were transformed with a. tumefaciens lba4404 harboring ... | 1998 | 9759540 |
| modeling and biological implication of time-dose-mortality data for the entomophthoralean fungus, zoophthora anhuiensis, on the green peach aphid myzus persicae | the entomophthoralean fungus zoophthora anhuiensis frequently causes epizootics in populations of the green peach aphid, myzus persicae, infesting cruciferous crops in the middle and lower regions of the changjiang river, china, during late autumn and early winter. in this study, a bioassay was conducted by exposing aphids on detached leaves to varying doses of z. anhuiensis conidia discharged from in vitro cultures. ten doses (1.5-198 conidia/mm2) were used, with each dose including 64-120 aphi ... | 1998 | 9784347 |
| analysis of clonal diversity of the peach-potato aphid, myzus persicae (sulzer), in scotland, uk and evidence for the existence of a predominant clone. | clones of the peach-potato aphid, myzus persicae (sulzer), mostly from scotland, uk were examined using an rdna fingerprinting technique. eighty patterns (genotypes) were found amongst the 276 clones. a large number of clones (30%) from all sample areas in scotland exhibited the same simple pattern, suggesting the presence of a single m. persicae clone. there was no difference in genotype distributions between m. persicae collected from brassica or potato crops, suggesting that host-adapted geno ... | 1998 | 9819902 |
| duplication of the rdl gaba receptor subunit gene in an insecticide-resistant aphid, myzus persicae. | resistance to cyclodiene insecticides is associated with replacements of a single amino acid (alanine 302) in a gamma-aminobutyric acid (gaba) receptor subunit encoded by the single-copy gene resistance to dieldrin (rdl). alanine 302 is predicted to reside within the second membrane-spanning region of the rdl receptor, a region that is thought to line the integral chloride ion channel pore. in all cyclodiene-resistant insects studied to date, this same alanine residue is replaced either by a ser ... | 1998 | 9862468 |
| aphid species--vectors of plum pox virus. | plum pox virus (ppv) is widely spread by natural vectors present in plum orchards. the efficiency of transmission is dependent on the frequency of the occurrence of vectors and the cultivar susceptibility to this pathogen. having in view that ppv has a wide range of annual and multiannual host plants and vectors, there is great concern for obtaining ppv-resistant cultivars. this report deals with the following vectors: hyalopterus pruni, brachycaudus cardui, brachycaudus helichrysi. myzus persic ... | 1998 | 10073225 |
| relationship between amount of esterase and gene copy number in insecticide-resistant myzus persicae (sulzer). | overproduction of the insecticide-degrading esterases, e4 and fe4, in peach-potato aphids, myzus persicae (sulzer), depends on both gene amplification and transcriptional control, the latter being associated with changes in dna methylation. the structure and function of the aphid esterase genes have been studied but the determination of their copy number has proved difficult, a common problem with gene amplification. we have now used a combination of pulsed-field gel electrophoresis and quantita ... | 1999 | 10215614 |
| differential mrna expression levels and gene sequences of a putative carboxylesterase-like enzyme from two strains of the parasitoid anisopteromalus calandrae (hymenoptera: pteromalidae). | carboxylesterase-like enzyme cdnas have been cloned and sequenced from malathion-resistant and susceptible strains of the parasitoid anisopteromalus calandrae (howard) (hymenoptera: pteromalidae). the cdnas consist of 1963 nucleotides including a 35 bp untranslated 5'-end, a 1596 bp open reading frame, and a 332 bp untranslated 3'-end. the open reading frame encodes 532 amino acid residues. the predicted protein sequence from these cdnas includes 2 potential n-glycosylation sites, a carboxyleste ... | 1999 | 10380653 |
| molecular characterization and imidacloprid selectivity of nicotinic acetylcholine receptor subunits from the peach-potato aphid myzus persicae. | the recent introduction of the chloronicotinyl insecticide imidacloprid, targeting insect nicotinic acetylcholine receptors (nachrs), emphasises the importance of a detailed molecular characterisation of these receptors. we are investigating the molecular diversity of insect nachr subunit genes in an important agricultural pest, the peach-potato aphid myzus persicae. two m. persicae alpha-subunit cdnas, mp alpha1 and mp alpha2, have been cloned previously. here we report the isolation of three n ... | 1999 | 10386991 |
| a sodium channel point mutation is associated with resistance to ddt and pyrethroid insecticides in the peach-potato aphid, myzus persicae (sulzer) (hemiptera: aphididae). | the voltage-gated sodium channel is the primary target site of ddt and pyrethroid insecticides, and point mutations in the domain ii region of the channel protein have been implicated in the knockdown resistant (kdr ) phenotype of several insect species. here, we report that one of these mutations, a leucine-to-phenylalanine replacement in transmembrane segment iis6, is also found in certain insecticide-resistant clones of the peach-potato aphid, myzus persicae. the mutation was present in four ... | 1999 | 10469251 |
| a solvent-free, rapid and simple virus rna-release method for potato leafroll virus detection in aphids and plants by reverse transcription polymerase chain reaction. | a one-step, rapid and economical method for potato leafroll virus (plrv) rna release that is applicable to the use on a microcentrifuge scale is described. discs (3-6 mm diameter) from leaves, petioles, stems, and tubers of potato plants were incubated in microcentrifuge tubes with detergent solution. the supernatants were used directly for reverse transcription (rt) and polymerase chain reaction (pcr). of the seven nonionic detergents of the tritonx series evaluated, triton x405r was the most e ... | 1999 | 10598080 |
| amplification and methylation of an esterase gene associated with insecticide-resistance in greenbugs, schizaphis graminum (rondani) (homoptera: aphididae). | the greenbug aphid, schizaphis graminum (rondani) has developed resistance to organophosphorus insecticides by the over-production of esterases that have been classified as type i and type ii. the first twenty n-terminal amino acids of the type i esterase were determined and used to design an oligonucleotide, which in conjunction with an active site primer derived from conserved sequences of other insect esterases and two internal primers specific for esterases from another aphid species resulte ... | 1999 | 10612041 |
| effects of point mutations in the readthrough domain of the beet western yellows virus minor capsid protein on virus accumulation in planta and on transmission by aphids. | point mutations were introduced into or near five conserved sequence motifs of the readthrough domain of the beet western yellows virus minor capsid protein p74. the mutant virus was tested for its ability to accumulate efficiently in agroinfected plants and to be transmitted by its aphid vector, myzus persicae. the stability of the mutants in the agroinfected and aphid-infected plants was followed by sequence analysis of the progeny virus. only the mutation y201d was found to strongly inhibit v ... | 2000 | 10627524 |
| cytogenetic and molecular characterization of a highly repeated dna sequence in the peach potato aphid myzus persicae. | electrophoresis following digestion of myzus persicae genomic dna with hindiii showed the presence of a prominent band of approximately 200 bp whereas a faint electrophoretic band corresponding to dna fragments of about 3000 bp was observed after digestion with apai. in situ digestion with restriction enzymes, followed by in situ nick translation, showed that apai targets are localized at the nucleolus organizer-bearing x telomeric region, whereas hindiii restriction sites are clustered in inter ... | 1999 | 10654082 |
| a member of the hsp60 gene family from the peach potato aphid, myzus persicae (sulzer.). | a novel cdna clone encoding a mitochondrial hsp60 was isolated from a myzus persicae cdna library. the nucleotide sequence consisted of 2348 bp and contained an open reading frame (orf) of 1722 bases. the putative protein encoded by this orf consisted of 574 amino acids and was designated mphsp60. comparison of mphsp60 with other hsp60s found that it was most similar to hsp60 from culicoides variipennis (85.6% similarity). phylogenetic analysis revealed that mphsp60 is clustered together with ot ... | 2000 | 10762429 |
| cloning, heterologous expression and co-assembly of mpbeta1, a nicotinic acetylcholine receptor subunit from the aphid myzus persicae. | nicotinic acetylcholine receptors (nachrs) play a major role in excitatory synaptic transmission in insects and are also the target site for chloronicotinyl insecticides such as imidacloprid. here we report the cloning and characterization of a novel nachr beta subunit, mpbeta1, from the aphid myzus persicae, an economically important pest species. sequence analysis has identified an open reading frame of 509 amino acids with features typical of nachr subunits. the mpbeta1 gene is expressed as a ... | 2000 | 10771176 |