Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| factors influencing the infection of cowpea protoplasts by cowpea mosaic virus rna. | 1976 | 7877 | |
| the ultrastructural appearance of cowpea mosaic virus in cowpea. | 1975 | 47914 | |
| complementation and pseudorecombination between ribonucleic acids from two natural isolates of cowpea mosaic virus (severe subgroup). | 1978 | 77077 | |
| efficient reverse transcription of cowpea mosaic virus rnas. | conditions are described which give an efficient synthesis of dna copies of cowpea mosaic virus (cpmv) rnas, using avian myeloblastosis reverse transcriptase and oligo (dt) primers. maximum incorporation of damp into cdna is attained with 0.4 to 0.8 mm of each deoxynucleoside triphosphate, 12 mm mg++ and 60 mm k+ ion concentrations. high enzyme concentrations (up to 100 units/ml) were used. under these conditions over 1000 pmoles of damp were incorporated per reaction. the cdna:rna molar ratio ... | 1978 | 85293 |
| sequence homology adjacent to the 3' terminal poly(a) of cowpea mosaic virus rnas. | we have determined the sequence of 80 nucleotides adjacent to 3' poly(a) of both (middle and bottom component) cowpea mosaic virus rnas, using dideoxynucleotide termination of reverse transcription. sequence conservation is indicated, there being about 80% homology between the first 65 bases of each rna. although both rnas are polyadenylated, there is no aauaaa sequence as associated with most known polyadenylated mrnas of eukaryotes and viruses. however both rnas are u and a-rich in this region ... | 1979 | 91160 |
| nucleotide sequences adjacent to the proteins covalently linked to the cowpea mosaic virus genome. sequence determination after labelling in vitro using rna ligase. | the sequences of the first 17 nucleotides of cowpea mosaic virus middle and bottom rnas adjacent to the covalently-linked proteins have been determined. sequences of the oligonucleotides, produced by complete t1 rnase digestion, were established after labelling of the 3' termini in vitro using rna ligase. both sequences are a/u-rich, the first nine nucleotides being identical. | 1979 | 116852 |
| the pattern of amino acid incorporation into two cowpea mosaic virus proteins in the presence of ribosome-specific protein synthesis inhibitors. | 1975 | 124497 | |
| protein bound to the genome rnas of cowpea mosaic virus. | 1984 | 215411 | |
| infection of cowpea mesophyll protoplasts with cowpea mosaic virus. | 1975 | 237360 | |
| tumor induction by agrobacterium tumefaciens prevented in vigna sinensis seedlings systemically infected by ribonucleic acid viruses. | cowpea (vigna sinensis) seedlings failed to develop tumors after being inoculated with crown gall bacteria (agrobacterium tumefaciens) if, at times earlier than 1 day later, they were inoculated on the primary leaves with a cowpea mosaic virus that systemically infects them. inoculation with buffer or with a virus that is restricted to a localized infection, or to which the cowpea is immune, did not interfere with the subsequent development of tumors. the virus infection did not appear to affect ... | 1979 | 422243 |
| the inhibition of cowpea mosaic virus replication by actinomycin d. | 1979 | 425319 | |
| synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. | 1979 | 462814 | |
| influence of virus infection on the leaf protein yield. | influence of cowpea mosaic virus (cpmv) and common bean mosaic virus (cbmv) infection on the yield of extractable leaf protein of cowpea cvs. pusa phaguni, pusa barsati, and yard long was studied. an increase as well as decrease in the yield of leaf protein concentrate (lpc), due to virus infection, has been observed. cpmv infection increased lpc yield in all the cowpea cvs., but cbmv decreased it in cys. pusa phaguni and yard long. variation in percentage of extractable nitrogen and protein in ... | 2005 | 549391 |
| a protein linked to the 5' termini of both rna components of the cowpea mosaic virus genome. | evidence is presented for the presence of a protein covalently bound to the 5' termini of both m and b rna components of cpmv. the protein is found to be linked in both cases to the 5' phosphate of the dinucleotide pupap, derived by ribonuclease digestion of the rna. the intact protein is not required for infectivity or for in vitro translation of the rna in cell-free extracts. | 1978 | 745988 |
| an efficient mrna-dependent translation system from reticulocyte lysates. | a simple method is described for converting a standard rabbit reticulocyte cell-free extract (lysate) into an mrna-dependent protein synthesis system. the lysate is preincubated with cacl2 and micrococcal nuclease, and then excess ethyleneglycol-bis(2-aminoethylether)-n,n'-tetraacetic acid is added to chelate the ca2+ and inactivate the nuclease. lysates treated in this way have neglibible endogenous amino acid incorporation activity, but 75% of the activity of the original lysate can be recover ... | 1976 | 823012 |
| genetic and physiological characterization of a temperature-sensitive mutant of cowpea mosaic virus. | 1977 | 835227 | |
| translation of cowpea mosaic virus rna in a cell-free extract from wheat germ. | 1977 | 862928 | |
| location of the gene specifying the smaller protein of the cowpea mosaic virus capsid. | 1977 | 867824 | |
| cowpea mosaic virus rnas have neither m7gpppn ... nor mono-, di- or triphosphates at their 5' ends. | 32p-labeled cowpea mosaic virus (cpmv) rnas were analyzed for their 5' termini by three different fingerprinting standard procedures. these analyses showed unequivocally that cpmv rnas lack a structure like m7gpppn ... at their 5' termini. in addition, neither mono-, di- or triphosphorylated [(p)(p)pn ....] termini were found in the respective fingerprints. the inability to label the 5' termini treatment with gamma-32p-atp and polynucleotide kinase, either per se or after pretreatment with alkal ... | 1977 | 872220 |
| electrophoretic mobility and enhanced infectivity of a mutant of cowpea mosaic virus. | 1976 | 936474 | |
| the use of an abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virus. | 1975 | 1114711 | |
| in vitro replication of cowpea mosaic virus rna. ii. solubilization of membrane-bound replicase and the partial purification of the solubilized enzyme. | a method for the solubilization of membrane-bound cowpea mosaic virus rna replicase has been developed by bypassing the use of detergents. solubilization has been achieved by washing the 31,000 x g-pellet containing the bound replicase with a mg2+-deficient buffer. this procedure had several advantages as compared to treatments with nonionic or ionic detergents: (i) the solubilized enzyme was stable at 4 c, (ii) more than 80% of the replicase could be solubilized without loss of total enzyme act ... | 1976 | 1255853 |
| genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment tests. | 1976 | 1258375 | |
| proceedings: properties of the template-dependent cowpea mosaic virus replicase. | 1976 | 1275384 | |
| interaction between viruses and monoclonal antibodies studied by surface plasmon resonance. | an automated biosensor system designed for measuring molecular interactions in real time and without any labelling of the reactants has been used to study the interaction of two animal viruses (vaccinia virus and poliovirus) and two plant viruses (cowpea mosaic virus and tobacco mosaic virus) with monoclonal antibodies. using monoclonal antibodies specific for different conformational states of viral protein, it was found that the virus particles retained their conformational integrity when immo ... | 1992 | 1372280 |
| the nucleotide sequence of red clover mottle virus bottom component rna. | the complete nucleotide sequence of the bottom component rna (b rna) of red clover mottle virus strain s has been determined. the sequence consists of 6033 nucleotides and contains a single long open reading frame sufficient to encode a protein of m(r) 210,258. the proteolytic processing sites within this protein have been deduced by comparison of its sequence with that of the b rna-encoded protein of cowpea mosaic virus. comparison of the amino acid sequences of the individual proteins confirms ... | 1992 | 1402822 |
| a regulatory role for the 32k protein in proteolytic processing of cowpea mosaic virus polyproteins. | we have studied the regulation of proteolytic processing of the polyproteins encoded by cowpea mosaic virus m-rna and b-rna. for that purpose mutations were introduced in full-length cdna clones of these rnas. rna transcripts were translated in rabbit reticulocyte lysate and the effect of mutations on the processing was analysed. these studies revealed that the 32k protein is released from the 200k b-polyprotein by an intramolecular cleavage and remains associated with the 170k protein, probably ... | 1992 | 1413528 |
| processing of vpg-containing polyproteins encoded by the b-rna from cowpea mosaic virus. | to study the processing of putative vpg precursors the expression of specific mutant transcripts derived from a full-length cdna clone of cowpea mosaic virus (cpmv) b-rna was examined in a rabbit reticulocyte lysate system. this study revealed that the 170k protein produced by a b-rna mutant that lacks the 32k coding region was efficiently processed by mainly intramolecular cleavages at three different sites into three sets of proteins of 60k + 110k, 84k + 87k, and 58k + 112k. further cleavage o ... | 1992 | 1413529 |
| structure determination of the bacteriophage phix174. | the structure of the single-stranded dna phage phix174 has been determined to 3.4 a resolution. the crystal space group was p2(1) with one icosahedral particle per asymmetric unit, giving 60-fold noncrystallographic redundancy. oscillation diffraction photographs were collected using synchrotron radiation at various wavelengths. the particle orientations in the unit cell were determined with a rotation function. because cowpea mosaic virus has a similar external envelope to phix174, it was used ... | 1992 | 1418820 |
| synthesis of the complete 200k polyprotein encoded by cowpea mosaic virus b-rna in insect cells. | the coding sequence for the entire 200k polyprotein of cowpea mosaic virus (cpmv) b-rna was expressed in insect cells by using baculovirus expression vectors. the 200k polyprotein, which harbours all virus functions required for rna replication, is completely cleaved into 170k and 32k products by the 24k protease activity contained within the polyprotein. further processing of the 170k protein into cpmv-specific products of 60k, 84k, 87k, 110k and 112k occurred to a limited extent, similar to th ... | 1992 | 1431806 |
| cloned dna copies of cowpea severe mosaic virus genomic rnas: infectious transcripts and complete nucleotide sequence of rna 1. | cowpea severe mosaic virus (cpsmv) is a member of the comovirus group of messenger-sense rna viruses with bipartite genomes, of which cowpea mosaic virus (cpmv) is the type member. full-length copies of cpsmv rna 1 were cloned in plasmids bearing a bacteriophage t7 promoter. previously, similar clones of cpsmv rna 2 had been obtained. a 5'-ruauuaaaauuuu sequence is common to rna 1 and rna 2. from two rna 1 clones and four rna 2 clones we excised non-cpsmv sequences so as to provide templates for ... | 1992 | 1448917 |
| a proposal for a bean common mosaic subgroup of potyviruses. | in order to elucidate the taxonomic positions of bean common mosaic virus (bcmv) and blackeye cowpea mosaic virus (b1cmv), several strains of these viruses were compared on the basis of host ranges, antigenic properties established with antisera to virions and to n-terminal peptide domains of their coat proteins, and high performance liquid chromatographic peptide profiles. the comparison includes three strains of bcmv, viz. nl1, nl3 and ny15, four strains of b1cmv, viz. fla, ind, nr, and w, and ... | 1992 | 1450765 |
| serological and biological relationships among viruses in the bean common mosaic virus subgroup. | bean common mosaic virus (bcmv), blackeye cowpea mosaic virus (b1cmv), cowpea aphid-borne mosaic virus (cabmv), azuki bean mosaic virus (azmv), and peanut stripe virus (pstv) are five species of the genus potyvirus, family potyviridae which are seed-transmitted in beans or cowpeas. eighteen isolates of bcmv, five isolates of b1cmv, four isolates of cabmv, and one isolate each of azmv, and pstv were compared serologically using a panel of 13 monoclonal antibodies (mabs) raised against bcmv, b1cmv ... | 1992 | 1450766 |
| strains of bean common mosaic virus consist of at least two distinct potyviruses. | bean common mosaic virus (bcmv) consists of a large number of pathotypes and strains which have largely been identified by their characteristic interactions with a selected number of differential bean cultivars. the relationships among these strains and other potyviruses that infect legumes are complex, with indications that bcmv, blackeye cowpea mosaic virus (blcmv) and azuki bean mosaic virus (azmv) may be strains of the one virus. using high performance liquid chromatographic peptide profiles ... | 1992 | 1450767 |
| coat protein properties suggest that azuki bean mosaic virus, blackeye cowpea mosaic virus, peanut stripe virus, and three isolates from soybean are all strains of the same potyvirus. | the interrelationship of a number of potyviruses infecting legumes has been investigated by comparing molecular properties of their coat proteins. comparison of the coat proteins by the techniques of amino acid analysis and page was inadequate to distinguish strains from distinct potyviruses. however, high-performance liquid chromatographic peptide profiles of tryptic digests of coat proteins of these legume-infecting potyviruses enabled such assignments to be made. these data indicate that amin ... | 1992 | 1500273 |
| effects of site-directed mutagenesis on the presumed catalytic triad and substrate-binding pocket of grapevine fanleaf nepovirus 24-kda proteinase. | grapevine fanleaf nepovirus (gflv) has a bipartite plus-sense rna genome. its structural and functional proteins originate from polyprotein maturation by at least one virus-encoded proteinase. here we describe the cloning of the 24-kda proteinase cistron located between the virus-linked protein (vpg) and the rna-dependent rna polymerase cistron in gflv rna1 (nucleotides 3966 to 4622). proteinase expressed from this clone is able to cleave gflv polyprotein p2 in order to produce the coat protein ... | 1992 | 1519363 |
| sequence upstream of the 24k protease enhances cleavage of the cowpea mosaic virus b rna-encoded polyprotein at the junction between the 24k and 87k proteins. | to investigate cleavage at the junction between the cowpea mosaic virus (cpmv) 24k and 87k proteins, plasmids were constructed containing the sequence of bottom-component (b) rna encoding the 110k protein plus a variable length of upstream coding sequence. transcripts derived from these clones were translated in rabbit reticulocyte lysate and the appearance of the 87k protein was used to assess the efficiency of cleavage at the 24k-87k junction. the results show that the 110k protein, containing ... | 1992 | 1604812 |
| expression of cowpea mosaic virus coat protein precursor in transgenic tobacco plants. | tobacco, nicotiana tabacum l., supports cowpea mosaic virus (cpmv) replication and cell-to-cell movement, and thus may serve as a model system to study coat protein-mediated protection against cpmv. a chimeric gene consisting of the cauliflower mosaic virus 35s promoter, cpmv 60k coat proteins-precursor (cp-p) coding region, and the nopaline synthase polyadenylation signal was transferred to tobacco cv. burley 21 via the agrobacterium tumefaciens binary vector system. gene integration and expres ... | 1992 | 1730936 |
| identification of a fab interaction footprint site on an icosahedral virus by cryoelectron microscopy and x-ray crystallography. | biological processes frequently require the formation of multi-protein or nucleoprotein complexes. some of these complexes have been produced in homogeneous form, crystallized, and analysed at high resolution by x-ray crystallography (for example, see refs 1-3). most, however, are too large or too unstable to crystallize. individual components of such complexes can often be purified and analysed by crystallography. here we report how the coordinated application of cryoelectron microscopy, three- ... | 2000 | 1731227 |
| the mechanism of translation of cowpea mosaic virus middle component rna: no evidence for internal initiation from experiments in an animal cell transient expression system. | the possibility that internal initiation of translation is responsible for the synthesis of the middle component (m) rna-encoded 95k protein of cowpea mosaic virus (cpmv) has been investigated by constructing plasmids in which the entire sequence of cpmv m rna was cloned downstream of a chloramphenicol acetyltransferase gene. expression of these plasmids in an animal cell expression system revealed that no synthesis of the proteins encoded by the downstream cpmv open reading frame takes place fr ... | 1991 | 1765773 |
| evidence for dissimilar properties of comoviral and picornaviral rna polymerases. | the poliovirus rna polymerase has been synthesized in spodoptera frugiperda cells by using the baculovirus expression system. crude sonicates of these cells exhibited an rna-elongating activity of a synthetic oligo(u) primer with poly(a) or cowpea mosaic virus (cpmv) rna as a template. a similar polymerase activity was found in extracts of insect cells in which foot-and-mouth disease virus (fmdv) proteins, including the putative polymerase, were produced. the analogous cpmv 87k protein and sever ... | 1991 | 1848591 |
| potassium salts influence the fidelity of mrna translation initiation in rabbit reticulocyte lysates: unique features of encephalomyocarditis virus rna translation. | it is widely assumed that in vitro translation of mrna is more efficient in the presence of potassium acetate rather than kcl, that the optimum concentration of potassium acetate is higher than for kcl, and that uncapped rnas exhibit a lower optimum salt concentration than capped mrnas. when these assumptions were examined using several different mrna species in four batches of rabbit reticulocyte lysate, some notable exceptions were found. the translation of encephalomyocarditis virus (emcv) rn ... | 1991 | 1849749 |
| nucleotide sequence of tomato ringspot virus rna-2. | the sequence of tomato ringspot virus (tomrsv) rna-2 has been determined. it is 7273 nucleotides in length excluding the 3' poly(a) tail and contains a single long open reading frame (orf) of 5646 nucleotides in the positive sense beginning at position 78 and terminating at position 5723. a second in-frame aug at position 441 is in a more favourable context for initiation of translation and may act as a site for initiation of translation. the tomrsv rna-2 3' noncoding region is 1550 nucleotides ... | 1991 | 1856689 |
| mutational analysis of the putative catalytic triad of the cowpea mosaic virus 24k protease. | to investigate the mechanism of action of the cowpea mosaic virus (cpmv) 24k protease, a full-length cdna clone of bottom component (b) rna has been constructed from which rna can be transcribed in vitro using t7 rna polymerase. translation of the resulting rna in rabbit reticulocyte lysate leads to the synthesis of a 200 kda product (the 200k protein) which cleaves itself in a manner identical to that of the product translated from b rna isolated from virions. site-directed mutagenesis of the f ... | 1991 | 1887592 |
| the atp requirement for initiation of eukaryotic translation varies according to the mrna species. | the requirement for atp for initiation of eukaryotic mrna translation was tested using gel-filtered rabbit reticulocyte lysates incubated with labelled met-trnafmet and exogenous rna templates, and assaying the formation of labelled 80s initiation complexes in the presence of gtp, or labelled 40s initiation complexes in the presence of a non-hydrolysable analogue of gtp. initiation complex formation on globin mrna, or on capped viral rnas such as papaya mosaic virus rna and tobacco mosaic virus ... | 1991 | 1889398 |
| the sequence between nucleotides 161 and 512 of cowpea mosaic virus m rna is able to support internal initiation of translation in vitro. | cowpea mosaic virus m rna is translated in vitro as well as in vivo into two c-coterminal polyproteins of mr 105k and 95k. initiation of translation of the 95k protein gene occurs at an aug codon at position 512 of m rna, 351 nucleotides downstream of the initiation codon of the 105k protein gene at position 161. by employing an in vitro transcription and translation system it was determined that this 351 nucleotide sequence has the capacity to direct ribosomes to initiate translation at a downs ... | 1991 | 1919521 |
| tubular structures involved in movement of cowpea mosaic virus are also formed in infected cowpea protoplasts. | in cowpea plant cells infected with cowpea mosaic virus, tubular structures containing virus particles are formed in the plasmodesmata between adjacent cells; these structures are supposedly involved in cell-to-cell spread of the virus. here we show that similar tubular structures are also formed in cowpea protoplasts, from which the cell wall and plasmodesmata are absent. between 12 and 21 h post-inoculation, tubule formation starts in the periphery of the protoplast at the level of the plasma ... | 1991 | 1940857 |
| vpg-mediated aggregation of potyviral rna. | rna prepared from the potyvirus tobacco vein mottling virus contained aggregates of the 9.5 kb genomic rna with electrophoretic mobilities corresponding to 20 and 41 kb species. similar aggregates were present in preparations of the rnas of two other potyviruses. aggregation occurred during or after purification of the rna by sucrose gradient centrifugation and alcohol precipitation and was dependent upon the presence of a protein apparently bound covalently to a region at or near the 5' terminu ... | 1991 | 1990064 |
| cowpea mosaic virus middle component rna contains a sequence that allows internal binding of ribosomes and that requires eukaryotic initiation factor 4f for optimal translation. | cowpea mosaic virus (cpmv) middle component rna (m-rna) encodes two proteins of 105 and 95 kda, of which translation starts at nucleotide (nt) 161 and nt 512, respectively. in vitro translation of both proteins directed by t7 transcripts of m-rna was stimulated fourfold by eukaryotic initiation factor 4f (eif-4f), the cap-binding protein complex. the ratio of the synthesis of both proteins after translation was not influenced by eif-4f or by any known eif. part of the cpmv 5' sequence was cloned ... | 1991 | 2033661 |
| the primary structure of the 24k protease from red clover mottle virus: implications for the mode of action of comovirus proteases. | we have determined the nucleotide sequence of the region of red clover mottle virus (rcmv) bottom component rna which encodes the rcmv equivalent of the cowpea mosaic virus (cpmv) 24k protease. from the alignment of the deduced amino acid sequence of the rcmv 24k protein with that of the homologous protein from cpmv, we speculate on the relative importance of the various amino acid residues which have been implicated in the catalytic mechanism of comovirus proteases. | 1990 | 2179468 |
| expression of plant virus genes in animal cells: high-level synthesis of cowpea mosaic virus b-rna-encoded proteins with baculovirus expression vectors. | the baculovirus expression system has been used to produce non-structural proteins encoded by bottom-component rna (b-rna) of cowpea mosaic virus (cpmv). for this, cdnas containing the 60k, 87k, 110k and 170k protein coding sequences were each provided with an atg start codon and the cdna containing the 60k coding sequence with a taa stop codon immediately downstream of the coding sequence. recombinant baculoviruses were retrieved which harboured the modified b-cdna sequences under the control o ... | 1990 | 2254749 |
| computer-aided assignment of the 1h-nmr spectrum of the viral-protein-genome-linked polypeptide from cowpea mosaic virus. | the 1h-nmr spectrum of the viral-protein-genome-linked (vpg) polypeptide from cowpea mosaic virus, has been interpreted via application of 2-dimensional (2d) nmr techniques. the interpretation of the data was performed by a computer program called 'prospect' (protein spectra), which detects the cross-peak patterns of the amino acid residues in the spectra, assigns these patterns to amino acid types, and finally performs the sequential assignments using the well-known 'sequential walks' obtained ... | 1990 | 2373083 |
| isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (-) strand viral dna and reverse transcription of primed rna templates. | sub-cellular fractions, isolated from cauliflower mosaic virus (camv)-infected turnip protoplasts, are capable of synthesising camv dna in vitro on an endogenous template and of reverse transcribing oligo dt-primed cowpea mosaic virus rna. the activity was not detected in mock-inoculated protoplasts. in vitro-labelled dna hybridized to single-stranded m13 clones complementary to the putative origins of (-) and (+) strand camv dna synthesis and to restriction endonuclease fragments encompassing m ... | 1985 | 2409536 |
| high-level synthesis of cowpea mosaic virus rna polymerase and protease in escherichia coli. | an expression system for the production of polymerase proteins of cowpea mosaic virus (cpmv) in escherichia coli cells is described. high-level synthesis of proteins containing protease and polymerase moieties (110-kda protein) and polymerase alone (87-kda protein) were obtained from cells containing different plasmid constructions. precursor and processed forms of cpmv proteins were detected by immunoblotting with antisera directed against 170-kda precursor polyprotein and 24-kda viral protease ... | 1989 | 2548928 |
| identification and subcellular localization of a putative cell-to-cell transport protein from red clover mottle virus. | to investigate the mode of gene expression of red clover mottle virus (rcmv) middle component (m) rna, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the rcmv counterparts of the cowpea mosaic virus (cpmv) 48k and 58k proteins. using an antiserum raised against this synthetic oligopeptide, we have detected a 43-kda protein in the 30,000 g pellet from extracts of rcmv-infected cowpea protoplasts. immunogold cytochemistry further localized this protein to th ... | 1989 | 2596020 |
| improvements of the infectivity of in vitro transcripts from cloned cowpea mosaic virus cdna: impact of terminal nucleotide sequences. | full-length dna copies of both b- and m-rna of cowpea mosaic virus (cpmv) were constructed downstream from a t7 promoter. by removal of nucleotides from the promoter sequence, b- and m-rna-like transcripts with varying numbers of additional nonviral sequences at the 5' end were obtained upon transcription with t7 rna polymerase. the infectivity of the transcripts in cowpea protoplasts was greatly affected by only a few extra nonviral nucleotides at the 5' end. the addition of about 400 nonviral ... | 1989 | 2596025 |
| analysis of sequences involved in cowpea mosaic virus rna replication using site-specific mutants. | using a full-length cdna clone of cowpea mosaic virus (cpmv) b-rna from which infectious transcripts can be generated, we examined the influence of a sequence of 11 nucleotides, uuuuauuaaaa, comprising the nucleotides 5883 to 5893 in the 3' noncoding region of b-rna, on viral rna replication. this sequence is not only present in b-rna but also in m-rna and represents the 7 nucleotides preceding the poly(a) tail and the first four a residues of the poly(a) tail. replication of b-rna transcripts d ... | 1989 | 2596026 |
| identification of the initiation codons for translation of cowpea mosaic virus middle component rna using site-directed mutagenesis of an infectious cdna clone. | a full-length cdna copy of cpmv m rna has been cloned downstream of a phage lambda promoter in the plasmid ppmi. transcripts obtained from this clone can be translated in vitro and replicated in cowpea mesophyll protoplasts in the presence of viral b rna. we have constructed a series of site-directed mutants of this clone to investigate the mechanism of translation of cpmv m rna. the results obtained confirm that the aug at position 161 is used to direct the synthesis of a 105k protein in vitro ... | 1989 | 2773321 |
| inhibition of proteolytic processing of the polyproteins of cowpea mosaic virus by hemin. | cleavages of the polyproteins synthesized from cowpea mosaic virus (cpmv) b rna and m rna in rabbit reticulocyte lysates are inhibited by hemin. cleavage of the cpmv b rna-encoded 200k polyprotein and of the m rna-encoded 60k intermediary precursor protein were most sensitive to hemin inhibition, while cleavages of other precursor proteins were less sensitive. a significant observation was that at a hemin concentration of 25 microm, but not at higher concentrations, the 60k protein was cleaved t ... | 1989 | 2815588 |
| cleavage of a viral polyprotein by a cellular proteolytic activity. | the 200,000-dalton polyprotein encoded by the bottom component rna of cowpea mosaic virus was synthesized in rabbit reticulocyte lysates, and this in vitro-synthesized protein was isolated from the lysate reaction mixture by sucrose density gradient centrifugation. incubation of the isolated polyprotein with buffer caused no change in the protein, but incubation with reticulocyte lysates or with fractionated lysate proteins resulted in cleavage of the protein into the expected cleavage products ... | 1986 | 3003387 |
| two viral proteins involved in the proteolytic processing of the cowpea mosaic virus polyproteins. | a series of specific deletion mutants derived from a full-length cdna clone of cowpea mosaic virus (cpmv) b rna was constructed with the aim to study the role of viral proteins in the proteolytic processing of the primary translation products. for the same purpose cdna clones were constructed having sequences derived from both m and b rna of cpmv. in vitro transcripts prepared from these clones with t7 rna polymerase, were efficiently translated in rabbit reticulocyte lysates. the translation pr ... | 1988 | 3282225 |
| proteolytic activity of the cowpea mosaic virus encoded 24k protein synthesized in escherichia coli. | the function of the 24-kilodalton (24k) protein encoded by cowpea mosaic virus (cpmv) has been studied by constructing a bacterial expression plasmid that contained a cloned chimeric segment consisting of partial dna copies of cpmv m-rna (including sequences coding for both capsid proteins) and b-rna (including sequences coding for the 24k protein). viral sequences were transcribed from the phage t7 promoter phi 10 of plasmid pt7-6 using t7-rna polymerase expressed from plasmid pgp1-2 present in ... | 1987 | 3300014 |
| analysis and application of substrate hydrolysis rates in indirect elisa of a purified plant virus. | the transient colorimetric signal in a microtiter plate is used to quantify a purified plant virus, cowpea mosaic virus (cpmv), over five concentration decades in a single plate. the method involves the coating of the polystyrene microtiter plate wells directly with the cpmv antigen, followed by incubation with a rabbit-derived cpmv-specific antibody, and lastly by incubation with a commercially available antibody against rabbit immunoglobulin which has been pre-labeled with alkaline phosphatase ... | 1988 | 3366853 |
| infectious rna transcripts derived from full-length dna copies of the genomic rnas of cowpea mosaic virus. | a set of full-length dna copies of both m and b rna of cowpea mosaic virus (cpmv) was cloned downstream of a phage t7 promoter. upon in vitro transcription using t7 rna polymerase, m and b rna-like transcripts were obtained from these dna copies with only two additional nucleotides at the 5' end and five extra nucleotides at the 3' end in comparison to natural viral rna. in cowpea protoplasts the transcripts of several cdna clones of b rna were able to replicate leading to detectable synthesis o ... | 1988 | 3388776 |
| molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses. | to investigate the degree of similarity between picornavirus proteases, we cloned the genomic cdnas of an enterovirus, echovirus 9 (strain barty), and two rhinoviruses, serotypes 1a and 14lp, and determined the nucleotide sequence of the region which, by analogy to poliovirus, encodes the protease. the nucleotide sequence of the region encoding the genome-linked protein vpg, immediately adjacent to the protease, was also determined. comparison of nucleotide and deduced amino acid sequences with ... | 1986 | 3512851 |
| the isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography. | this report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (fplc) techniques. the fplc is used here to separate the two electrophoretic forms of cowpea mosaic virus. the capsid forms are shown to be separated by the mono-q column without damaging the capsids. | 1987 | 3667850 |
| 1h, 13c, and 31p nuclear magnetic resonance studies of cowpea mosaic virus: detection and exchange of polyamines and dynamics of the rna. | 1h and 13c nmr studies on cowpea mosaic virus (cpmv) revealed that polyamines are present in the middle (m) and upper bottom (bu) components obtained by cscl density gradient centrifugation but not in the top (t) component; the lower bottom (bl) component contains trace amounts of polyamine. dialysis of the bl component against spermidine led to incorporation of spermidine which gave rise to nmr peaks very similar to those observed with the natural m and bu components. nmr results conclusively d ... | 1985 | 3976178 |
| thermal stability of cowpea mosaic virus components: differential scanning calorimetry studies. | differential scanning calorimetry studies show that the thermal stability of cowpea mosaic virus (cpmv) is ph dependent in the ph range 5 to 8. the four different components of cpmv, top (protein shell devoid of rna), middle (virus particle containing the smaller rna), bottom upper and bottom lower (virus particle containing the larger rna), are stable up to 64-70 degrees at ph 5.0 and up to 43-46 degrees at ph 8.25. these components did not exhibit any difference in their thermal stability betw ... | 1985 | 4036006 |
| structure in cells of vigna unguiculata infected with cowpea mosaic virus. | 1971 | 4108621 | |
| a three-dimensional image reconstruction of cowpea mosaic virus. | 1974 | 4131954 | |
| association of cowpea mosaic virus-induced double-stranded rna with a cytopathological structure in infected cells. | 1974 | 4364824 | |
| serological relationships among members of the cowpea mosaic virus group. | 1966 | 4380471 | |
| structure of isometric viruses containing nonidentical polypeptide chains. | the theory of caspar and klug (1962) for the structure of isometric viruses has been generalized to the case in which the identical repeating unit is composed of n nonidentical polypeptide chains. this modified theory accounts for the structure of picornaviruses, the lambda phage head, cowpea mosaic virus, and phix174, while at the same time conserving the principle of having identical subunits in identical environments. furthermore, the modified theory suggests amending the triangulation number ... | 1974 | 4418754 |
| in vitro replication of cowpea mosaic virus rna: i. isolation and properties of the membrane-bound replicase. | a fraction which contained the membrane-bound cowpea mosaic virus rna replicase was isolated from cowpea mosaic virus-infected cowpea leaves. the replicase activity appeared on day 1 after inoculation, then increased to reach a maximal on day 4. the increase in enzyme activity preceded the most-rapid virus multiplication. the membrane-bound replicase activity was almost completely insensitive to actinomycin d and dnase. the corresponding fraction from healthy leaves had no rna-dependent rna poly ... | 1974 | 4431078 |
| insect vectors of cowpea mosaic virus in nigeria. | 1974 | 4470367 | |
| the localization of virus-specific double-stranded rna of cowpea mosaic virus in subcellular fractions of infected vigna leaves. | 1973 | 4576343 | |
| the infectivity of the two electrophoretic forms of cowpea mosaic virus. | 2015 | 4693399 | |
| characterization of the double-stranded rna isolated from cowpea mosaic virus-infected vigna leaves. | 1973 | 4696555 | |
| polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. | 1973 | 4745624 | |
| infectivity of a mixture of cowpea mosaic virus ribonucleoprotein components. | 1967 | 4960987 | |
| conversion of the electrophoretic forms of cowpea mosaic virus in vivo and in vitro. | 1969 | 4979607 | |
| hydrogen ion effects on the thermal inactivation of cowpea mosaic virus. | 1971 | 5002942 | |
| structure of the capsid of cowpea mosaic virus. the chemical subunit: molecular weight and number of subunits per particle. | 1972 | 5039022 | |
| genetic complementation between the two nucleoprotein components of cowpea mosaic virus. | 1972 | 5053105 | |
| two proteins from cowpea mosaic virus. | 1971 | 5137799 | |
| the significance of the coat protein in infection by the electrophoretic forms of cowpea mosaic virus. | 1970 | 5422717 | |
| the relationship between the components of cowpea mosaic virus. ii. further characterization of the nucleoprotein components of cpmv. | 1970 | 5422719 | |
| the relationship between the components of cowpea mosaic virus. 3. location of genetic information for two biological functions in the middle component of cpmv. | 1970 | 5422720 | |
| buoyant density changes of cowpea mosaic virus components at different ph values. | 1971 | 5543838 | |
| the relationship between the components of cowpea mosaic virus. i. two ribonucleoprotein particles necessary for the infectivity of cpmv. | 1968 | 5640822 | |
| the inheritance of top component formation in cowpea mosaic virus. | 1969 | 5781861 | |
| the isolation of ribonuclease-resistant rna induced by cowpea mosaic virus: evidence for two double-stranded rna components. | 1969 | 5786160 | |
| purification and properties of the components of cowpea mosaic virus. | 1967 | 6022691 | |
| nitrate reductase activity in cowpea mosaic virus infected cowpea plant parts. | 1982 | 6184966 | |
| similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families. | the amino acid sequences deduced from the nucleic acid sequences of several animal picornaviruses and cowpea mosaic virus (cpmv), a plant virus, were compared. good homology was found between cpmv and the picornaviruses in the region of the picornavirus 2c (p2-x protein), vpg, 3c pro (proteinase) and 3d pol (rna polymerase) regions. the cpmv b genome was found to have a similar gene organization to the picornaviruses. a comparison of the 3c pro (proteinase) regions of all of the available picorn ... | 1984 | 6384934 |
| rna and capsid accumulation in cowpea protoplasts that are resistant to cowpea mosaic virus strain sb. | leaf protoplasts from the arlington line of cowpea (vigna unguiculata) support only a limited increase of cowpea mosaic virus strain sb (cpmv-sb), whereas cowpea severe mosaic virus, another member of the comovirus group, replicates efficiently in arlington cowpea protoplasts. cpmv-sb replicates efficiently in protoplasts of cowpea line blackeye 5. some characteristics of the virus-specific resistance of arlington protoplasts to cpmv-sb are reported. differences between progeny cpmv-sb from arli ... | 1984 | 6485253 |
| detection of double-stranded rna by serologically specific electron microscopy. | details of a procedure for detecting double-stranded rna (dsrna) in virus and viroid infected tissue extracts using serologically specific electron microscopy are given. a method for staining dsrna, based on in situ formation of uranyl phosphate, that consistently permits the examination of dsrna by electron microscopy without shadowing with heavy metals, is described. the method provides for routine assays for dsrna in crude extracts without the variable results associated with shadowing proced ... | 1984 | 6530480 |
| study of the genetic organisation of a plant viral rna genome by in vitro expression of a full-length dna copy. | the genetic approach for elucidating functions encoded by rna plant viruses has been hampered by the lack of methods to select desired mutants following random mutagenesis. an alternative might be to copy rna genomes into dna and use methods for site-directed mutagenesis to modify specific regions of the dna copy. transcription of the dna copy will subsequently produce viral rna with desired mutations. we have constructed a full-length dna copy of the smaller of the two cowpea mosaic virus (cpmv ... | 1984 | 6549293 |
| primary structure and gene organization of the middle-component rna of cowpea mosaic virus. | middle component rna (m rna) of cowpea mosaic virus (cpmv) was transcribed into cdna and double-stranded cdna was inserted into the ecori site of plasmid pbrh2. the nucleotide sequence of inserts was determined, after subcloning in bacteriophages m13mp7, m13mp8 or m13mp9, by the dideoxy chain termination method. the complete sequence of cpmv m rna, up to the poly(a) tail, is 3481 nucleotides long. the sequence contains a long open reading frame starting at nucleotide 161 from the 5' terminus and ... | 1983 | 6641721 |
| translational stability of plant viral rnas microinjected into living cells. influence of a 3'-poly(a) segment. | three different alternative structural features have been shown to be present at the 3' terminus of plant viral rnas: (a) a poly(a) track, (b) a trna-like structure, (c) no special structural or sequence characteristic. we have compared the translational stability after injection into frog oocytes of a representative of each type: (a) the small genomic rna (m-rna) of cowpea mosaic virus (cpmv), (b) the subgenomic mrna for coat protein (rna 4) of brome mosaic virus (bmv), (c) the subgenomic mrna ... | 1983 | 6681761 |