Publications

cloned dna copies of cowpea severe mosaic virus genomic rnas: infectious transcripts and complete nucleotide sequence of rna 1.	cowpea severe mosaic virus (cpsmv) is a member of the comovirus group of messenger-sense rna viruses with bipartite genomes, of which cowpea mosaic virus (cpmv) is the type member. full-length copies of cpsmv rna 1 were cloned in plasmids bearing a bacteriophage t7 promoter. previously, similar clones of cpsmv rna 2 had been obtained. a 5'-ruauuaaaauuuu sequence is common to rna 1 and rna 2. from two rna 1 clones and four rna 2 clones we excised non-cpsmv sequences so as to provide templates for ...	1992	1448917
nucleotide sequence and genetic map of cowpea severe mosaic virus rna 2 and comparisons with rna 2 of other comoviruses.	we report the nucleotide sequence of cowpea severe mosaic comovirus (cpsmv) genomic rna 2. the molecule is composed of 3732 nucleotide (nt) residues, exclusive of the polyadenylate at the 3' end. only one of the six reading frame registers has a long open reading frame, from nt 255 to nt 3260 in the polarity of encapsidated rna and corresponding to a polyprotein of 1002 amino acid residues (aa). as has been reported for other comoviruses, a second in-frame aug, at nt position 531, apparently als ...	1992	1546463
rna and capsid accumulation in cowpea protoplasts that are resistant to cowpea mosaic virus strain sb.	leaf protoplasts from the arlington line of cowpea (vigna unguiculata) support only a limited increase of cowpea mosaic virus strain sb (cpmv-sb), whereas cowpea severe mosaic virus, another member of the comovirus group, replicates efficiently in arlington cowpea protoplasts. cpmv-sb replicates efficiently in protoplasts of cowpea line blackeye 5. some characteristics of the virus-specific resistance of arlington protoplasts to cpmv-sb are reported. differences between progeny cpmv-sb from arli ...	1984	6485253
antigenic signature analysis reflects differences among plant virus isolates.	antigenic differences among cowpea severe mosaic virus (cpsmv) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen simultaneously with each antibody in the panel. an iterative procedure was used to align unknown cpsmv antigen concentrations from different antigen preparations to allow comparison ...	1993	7685770
partially folded states of the capsid protein of cowpea severe mosaic virus in the disassembly pathway.	the different partially folded states of the capsid protein that appear in the disassembly pathway of cowpea severe mosaic virus (cpsmv) were investigated by examining the effects of hydrostatic pressure, sub-zero temperatures and urea. the conformational states of the coat protein were analyzed by their intrinsic fluorescence, binding of bis(8-anilinonaphthalene-1-sulfonate) (bis-ans) and susceptibility to trypsin digestion. cpsmv could be disassembled by pressure at 2.5 kbar. intrinsic fluores ...	1997	9344752
complete nucleotide sequence of bean pod mottle virus rna1: sequence comparisons and evolutionary relationships to other comoviruses.	the complete nucleotide sequence of bean pod mottle comovirus (bpmv) rna 1 was determined. it is 5983 nucleotides long, excluding the poly(a) tail, and encodes a polyprotein of 1850 amino acid (aa) residues. multiple alignments of the deduced aa sequence of bpmv polyprotein with those of cowpea mosaic virus (cpmv), red clover mottle virus (rcmv) and cowpea severe mosaic virus (cpsmv) indicated that bpmv rna1 encodes the predicted set of five mature proteins: the equivalent of cpmv 32k protease c ...	1999	10403699
evidence for participation of rna 1-encoded elicitor in cowpea mosaic virus-mediated concurrent protection.	the cowpea (vigna unguiculata) line arlington, inoculated with cowpea mosaic virus (cpmv), showed no symptoms, and no infectivity or accumulation of capsid antigen was detected at several days after inoculation. coinoculation, but not sequential inoculation, of cpmv with similar concentrations of another comovirus; cowpea severe mosaic virus (cpsmv), resulted in reduced numbers of cpsmv-induced lesions. this apparent, cpmv-mediated reduction in number of cpsmv-induced infection centers was terme ...	2000	10639316
immune response induced in mice oral immunization with cowpea severe mosaic virus.	there is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. cowpea severe mosaic virus (cpsmv), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (vigna unguiculata) leaves infected with cpsmv both administered by gavage to swiss mice induced a humoral immune response. groups of 10 swiss mice (2-month-old females) were immunized orally with 10 dail ...	2002	12131924
cowpea mosaic virus-encoded protease does not recognize primary translation products of m rnas from other comoviruses.	the protease encoded by the large (b) rna segment of cowpea mosaic virus was tested for its ability to recognize the in vitro translation products of the small (m) rna segment from the comoviruses squash mosaic virus, red clover mottle virus, and cowpea severe mosaic virus (cpsmv, strains dg and ark), and from the nepovirus tomato black ring virus. like m rna from cowpea mosaic virus, the m rnas from squash mosaic virus, red clover mottle virus, cpsmv-dg, and cpsmv-ark were all translated into t ...	1982	16789228
ultrastructure of mixed plant virus infection: bean yellow mosaic virus with cowpea severe mosaic virus or cowpea mosaic virus in bean.	ultrastructural responses of bean leaf cells simultaneously infected with two morphologically distinct rna viruses, cowpea mosaic virus (cpmv) and bean yellow mosaic virus (bymv), or cowpea severe mosaic virus (csmv) and bymv, were studied in situ. the major effects on cells infected with two viruses included: (1) association of virus group-specific cytoplasmic inclusions characteristic of each virus; (2) close association of virions into specifically arranged aggregates in which cpmv or csmv ic ...	1983	18638866
diversity among isolates of cowpea severe mosaic virus infecting cowpeas in northeastern brazil.	eleven isolates of cowpea severe mosaic virus (cpsmv), a member of the genus comovirus, were selected from 50 samples collected of nine cowpea fields in northeastern brazil (piauí, ceará, rio grande do norte, paraíba, pernambuco, alagoas, sergipe, bahia, and distrito federal) and partially sequenced. the rna1 partial sequence, corresponding to the helicase, viral genome-linked protein, picornain 3c-like protease, and the rna-directed rna polymerase genes from cpsmv, had high identity among isola ...	2012	23007993
drought increases cowpea (vigna unguiculata [l.] walp.) susceptibility to cowpea severe mosaic virus (cpsmv) at early stage of infection.	the physiological and biochemical responses of a drought tolerant, virus-susceptible cowpea genotype exposed to drought stress (d), infected by cowpea severe mosaic virus (cpsmv) (v), and to these two combined stresses (dv), at 2 and 6 days post viral inoculation (dpi), were evaluated. gas exchange parameters (net photosynthesis, transpiration rate, stomatal conductance, and internal co2 partial pressure) were reduced in d and dv at 2 and 6 dpi compared to control plants (c). photosynthesis was ...	2016	27669396
photosynthetic and biochemical mechanisms of an ems-mutagenized cowpea associated with its resistance to cowpea severe mosaic virus.	the seed treatment of a cpsmv-susceptible cowpea genotype with the mutagenic agent ems generated mutagenized resistant plantlets that respond to the virus challenge by activating biochemical and physiological defense mechanisms. cowpea is an important crop that makes major nutritional contributions particularly to the diet of the poor population worldwide. however, its production is low, because cowpea is naturally exposed to several abiotic and biotic stresses, including viral agents. cowpea se ...	2017	27838815
label-free proteomic reveals that cowpea severe mosaic virus transiently suppresses the host leaf protein accumulation during the compatible interaction with cowpea (vigna unguiculata [l.] walp.).	viruses are important plant pathogens that threaten diverse crops worldwide. diseases caused by cowpea severe mosaic virus (cpsmv) have drawn attention because of the serious damages they cause to economically important crops including cowpea. this work was undertaken to quantify and identify the responsive proteins of a susceptible cowpea genotype infected with cpsmv, in comparison with mock-inoculated controls, using label-free quantitative proteomics and databanks, aiming at providing insight ...	2016	27934294
gel-free/label-free proteomic, photosynthetic, and biochemical analysis of cowpea (vigna unguiculata [l.] walp.) resistance against cowpea severe mosaic virus (cpsmv).	cowpea severe mosaic virus (cpsmv) causes significant losses in cowpea (vigna unguiculata) production. in this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype brs-marataoã and cpsmv. the leaf protein extracts from mock- (mi) and cpsmv-inoculated plantlets (v) were evaluated at 2 and 6days post-inoculation (dpi). data support the assumptions that in ...	2017	28502737