Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the nucleotide sequence of the s rna of impatiens necrotic spot virus, a novel tospovirus. | impatiens necrotic spot virus (insv) shares a number of properties with tomato spotted wilt virus (tswv), the type species of the genus tospovirus within the family bunyaviridae. insv, however, differs from tswv in plant host range and serology. in order to define the genomic structure and the taxonomic status of this tswv-like virus, the nucleotide sequence of its genomic s rna segment has been determined. the molecular data obtained demonstrate that, like tswv, insv has an ambisense s rna mole ... | 1992 | 1385787 |
| the m rna of impatiens necrotic spot tospovirus (bunyaviridae) has an ambisense genomic organization. | the nucleotide sequence of impatiens necrotic spot virus (insv) m rna was determined from cdna clones. the insv m rna was 4972 nucleotides in length with two open reading frames (orfs) in an ambisense genomic organization. the larger orf near the 3' end of the viral rna, coding for a protein with a predicted molecular weight of 124.9 kda, was in the viral complementary sense and produced the g2 and g1 proteins. a smaller orf in the viral sense was capable of coding for a 34.1-kda polypeptide, de ... | 1992 | 1585644 |
| serological comparison of tospovirus isolates from taiwan and india with impatiens necrotic spot virus and different tomato spotted wilt virus isolates. | tospovirus isolates from tomato and watermelon in taiwan and from peanut in india reacted neither with antibodies to tomato spotted wilt virus (tswv), nor with antibodies to impatiens necrotic spot virus (insv), in elisa and in electroblot immunoassays. additionally, all three tospovirus isolates had an n protein with a molecular weight of 32 kda as compared to 29 kda for the n protein to tswv and insv. the observed physicochemical and serological differences suggest the generation of a new, thi ... | 1993 | 7686004 |
| resistance to tospoviruses in nicotiana benthamiana transformed with the n gene of tomato spotted wilt virus: correlation between transgene expression and protection in primary transformants. | nicotiana benthamiana was transformed with the nucleoprotein (n) gene of an italian isolate of tomato spotted wilt virus (tswv). forty-five t1 primary transformant lines were analyzed for the expression of n protein and for resistance to tswv and three other tospoviruses: impatiens necrotic spot virus (insv), groundnut bud necrosis virus (gbnv), and groundnut ringspot virus (grsv). thirteen of these lines were further characterized. resistance to all tswv isolates tested was found in two lines. ... | 1995 | 7772806 |
| classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. | the nucleotide sequences of the nucleoprotein (n) genes of seven tospovirus isolates representing three serogroups were determined and used to establish phylogenetic parameters to delineate species within the tospovirus genus of the bunyaviridae. a high sequence divergence (55.9% identity at the nucleotide level) was observed between isolates of serogroup i (tomato spotted wilt virus) and isolates of serogroup iii (impatiens necrotic spot virus). the serogroup ii isolates take an intermediate po ... | 1993 | 8429298 |
| the complete nucleotide sequence and genome organization of the m rna segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. | the m rna of peanut bud necrosis virus (pbnv; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. it comprised two orfs in an ambisense organization and terminal inverted repeats. the 3' large orf (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kda which was identified as the glycoprotein precursor (gp) of the g1 and g2 glycoproteins. a comparison of the deduced amino acid sequence of gp revealed 37% identity and 58-59% simil ... | 1996 | 8811036 |
| an improved method for the detection of tospoviruses using the polymerase chain reaction. | a reverse transcription-polymerase chain reaction (rt-pcr)-based assay for the detection of tomato spotted wilt virus (tswv) has been improved and extended to enable the detection of additional tospoviruses. in addition to tswv-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (insv) and another which detects all tospoviruses tested, including tswv, insv, tomato chlorotic spot virus and groundnut ringspot virus. a ... | 1996 | 8919828 |
| completion of the impatiens necrotic spot virus genome sequence and genetic comparison of the l proteins within the family bunyaviridae. | the nucleotide sequence of the large (l) genome segment of impatiens necrotic spot virus (insv) has been determined, and herewith the complete nucleotide sequence of the whole, tripartite genome of this important tospovirus has been elucidated. the l rna is 8776 nucleotides long and of negative polarity, containing one large orf on the viral complementary strand. comparison of the deduced amino acid sequence of the insv l rna primary translation product (330.3 kda) with those of the l rnas of ot ... | 1997 | 9049402 |
| serological comparison of tospoviruses with polyclonal antibodies produced against the main structural proteins of tomato spotted wilt virus. | a new purification procedure for the tospoviruses of serogroups ii and iii was developed. sds-polyacrylamide gel electrophoresis of purified tomato spotted wilt virus (tswv; serogroup i), groundnut ringspot virus (grsv: serogroup ii), tomato chlorotic spot virus (tcsv; serogroup ii) and impatiens necrotic spot virus (insv; serogroup iii) showed that the glycoprotein g2 of serogroup ii members differs significantly in size from that of the serogroup i virus. western immunoblot analysis using poly ... | 1997 | 9170504 |
| characterization of the large (l) rna of peanut bud necrosis tospovirus. | the nucleocapsids purified from peanut plants systemically infected with peanut bud necrosis virus (pbnv), a member of the genus tospovirus, contained both viral(v) and viral complementary(vc) sense l rnas. defective forms of l rna containing 'core polymerase region' were observed. the full length l rna of pbnv was sequenced using overlapping cdna clones. the 8911 nucleotide l rna contains a single open reading frame (orf) in the vc strand, and encodes a protein of 330 kda. at the 5' and 3' term ... | 1998 | 9930194 |
| rt-pcr for detecting five distinct tospovirus species using degenerate primers and dsrna template. | rt-pcr procedures for detection of multiple species of tospovirus from plant tissues were investigated. downstream primers were designated from the 3' untranslated sequences of the s rna. an upstream primer was designated from the degenerated sequences of the nucleocapsid protein. approximately 450 bp dna fragments were detected when tomato spotted wilt virus (tswv)- or impatiens necrotic spot virus (insv)- infected tissues were examined. approximately 350 bp dna fragments were detected when wat ... | 2001 | 11445145 |
| purification and serological analyses of tospoviral nucleocapsid proteins expressed by zucchini yellow mosaic virus vector in squash. | a plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus (zymv) was used to express the nucleocapsid proteins (nps) of tospoviruses in planta. the open reading frames (orfs) of nps of different serogroups of tospoviruses, including tomato spotted wilt virus, impatiens necrotic spot virus, watermelon silver mottle virus, peanut bud necrosis virus, and watermelon bud necrosis virus (wbnv), were in frame inserted in between the p1 and hc-pro genes of the zymv ... | 2005 | 15992936 |
| specific and common changes in nicotiana benthamiana gene expression in response to infection by enveloped viruses. | microarrays derived from solanum tuberosum expressed sequence tags were used to test the hypothesis that genetically distinct enveloped viruses elicit unique changes in nicotiana benthamiana gene expression. the results of our study, which included sonchus yellow net virus (synv), a plant rhabdovirus that replicates in the nucleus of infected cells, and impatiens necrotic spot virus (insv), a plant bunyavirus that replicates in the cytoplasm, were consistent with this hypothesis. statistically s ... | 2005 | 16099921 |
| completion of the genome sequence of watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. | abstract the nucleotide sequence of the l rna of watermelon silver mottle virus (wsmov) was determined. combined with the previous work on m and s rnas, the whole genomic sequence of this member of the genus tospovirus was completed. the l rna is 8,917 nucleotides in length, with one large open reading frame encoding a translation product of 2,878 amino acids (331.8 kda) on the viral complementary strand. the l protein shares amino acid identities of only 44.3 and 46.5% with tomato spotted wilt ... | 2001 | 18943848 |
| a one-step reverse transcription-polymerase chain reaction system for the simultaneous detection and identification of multiple tospovirus infections. | abstract a one-step reverse transcription-polymerase chain reaction (rt-pcr) method has been developed for the simultaneous detection and identification of multiple tospoviruses that infect plants. the rt-pcr system is composed of six primers in a single tube: a universal degenerate primer and five virus species-specific primers. amplifications resulted in an 848-bp pcr product for watermelon silver mottle virus, 709-bp for tomato spotted wilt virus, 589-bp for impatiens necrotic spot virus, 511 ... | 2005 | 18943986 |
| serological and molecular characterization of peanut chlorotic fan-spot virus, a new species of the genus tospovirus. | abstract to clarify the serological relationship of peanut chlorotic fan-spot virus (pcfv) with other tospoviruses, antisera were produced against the nucleocapsid (n) proteins of this virus and tospoviruses from four serogroups including tomato spotted wilt virus (tswv), impatiens necrotic spot virus (insv), groundnut ringspot virus (grsv), and watermelon silver mottle virus (wsmov). in immunodiffusion tests, the antisera only reacted with their homologous antigens. similar results were noticed ... | 2001 | 18944231 |
| application of phage display in selecting tomato spotted wilt virus-specific single-chain antibodies (scfvs) for sensitive diagnosis in elisa. | abstract a panel of recombinant single-chain antibodies (scfvs) against structural proteins of tomato spotted wilt virus (tswv) was retrieved from a human combinatorial scfv antibody library using the novel phage display technique. after subcloning the encoding dna sequences in the expression vector pskap/s, which allowed the scfvs to be expressed as alkaline phosphatase fusion proteins, 17 different scfv antibodies were obtained. of these, 12 scfvs were directed against the nucleoprotein (n) an ... | 2000 | 18944607 |
| comparison of ambisense m rna of watermelon silver mottle virus with other tospoviruses. | abstract double-stranded genomic rnas (dsrnas) extracted from chenopodium quinoa infected with watermelon silver mottle virus (wsmv) were similar to those of tomato spotted wilt virus (tswv, serogroup i) and impatiens necrotic spot virus (insv, serogroup iii), except that the s dsrna of wsmv is 0.75 and 0.6 kbp longer than those of tswv and insv, respectively. the complete nucleotide sequence of the genomic m rna of wsmv was determined from cdna clones generated from separated m dsrna. the m rna ... | 1998 | 18944959 |
| development of efficient plant regeneration and transformation system for impatiens using agrobacterium tumefaciens and multiple bud cultures as explants. | impatiens (impatiens walleriana) is a top selling floriculture crop. the potential for genetic transformation of impatiens to introduce novel flower colors or virus resistance has been limited by its general recalcitrance to tissue culture and transformation manipulations. we have established a regeneration and transformation system for impatiens that provides new alternatives to genetic improvement of this crop. | 2010 | 20696066 |
| diverging affinity of tospovirus rna silencing suppressor proteins, nss, for various rna duplex molecules. | the tospovirus nss protein was previously shown to suppress the antiviral rna silencing mechanism in plants. here the biochemical analysis of nss proteins from different tospoviruses, using purified nss or nss containing cell extracts, is described. the results showed that all tospoviral nss proteins analyzed exhibited affinity to small double-stranded rna molecules, i.e., small interfering rnas (sirnas) and micro-rna (mirna)/mirna* duplexes. interestingly, the nss proteins from tomato spotted w ... | 2010 | 20739523 |
| tospoviruses in the mediterranean area. | tospoviruses are among the most serious threats to vegetable crops in the mediterranean basin. tospovirus introduction, spread, and the diseases these viruses cause have been traced by epidemiological case studies. recent research has centered on the close relationship between tospoviruses and their arthropod vectors (species of the thripidae family). here, we review several specific features of tospovirus-thrips associations in the mediterranean. since the introduction of frankliniella occident ... | 2012 | 22682175 |
| development of a real-time fluorescent quantitative pcr assay for detection of impatiens necrotic spot virus. | impatiens necrotic spot virus (insv) is an important plant virus that can cause severe disease in various ornamental and agricultural crops. several species of thrips transmit insv, of which the western flower thrip (frankliniella occidentalis) is the most important. in this study, primers and taqman probes based on insv non-structural protein gene sequences were designed, and a technique was developed for detecting insv using fluorescent quantitative rt-pcr. the reproducibility, specificity and ... | 2013 | 23466630 |
| development of apple latent spherical virus-based vaccines against three tospoviruses. | apple latent spherical virus (alsv) is characterized by its relatively broad host range, latency in most host plants, and ability to induce gene silencing in host plants. herein, we focus on the above characteristic of alsv and describe our development of alsv vector vaccines against three tospoviruses, namely, impatiens necrotic spot virus (insv), iris yellow spot virus (iysv), and tomato spotted wilt virus (tswv). dna fragments of 201 nt of three tospovirus s-rnas (silencing suppressor (nss) a ... | 2013 | 23850843 |
| broad-spectrum transgenic resistance against distinct tospovirus species at the genus level. | thrips-borne tospoviruses cause severe damage to crops worldwide. in this investigation, tobacco lines transgenic for individual wlm constructs containing the conserved motifs of the l rna-encoded rna-dependent rna polymerase (l) gene of watermelon silver mottle virus (wsmov) were generated by agrobacterium-mediated transformation. the wlm constructs included: (i) translatable wlm in a sense orientation; (ii) untranslatable wlmt with two stop codons; (iii) untranslatable wlmts with stop codons a ... | 2014 | 24811071 |
| resistance breaking tomato spotted wilt virus isolates on resistant pepper varieties in italy. | in spring 2012, resistance breaking (rb) isolates of tomato spotted wilt virus (tswv) that overcome the resistance conferred by the tsw gene in different pepper hybrids have been recovered in different locations in southern italy (campania and apulia regions) in protected cultivation, about one month after transplant. the percentage of symptomatic plants was 5-10% and, only in particular cases of advanced stage of cultivation, it reached 30-50% at the end of cycle. all tswv isolates induced simi ... | 2013 | 25151838 |
| genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus tospovirus. | chrysanthemum stem necrosis virus (csnv) is a member of a tentative tospovirus species. in this study, the complete genomic sequence of the japanese csnv isolate tcch07a was determined. the l rna is 8960 nt long and encodes the 331.0-kda rna-dependent rna polymerase. the m rna is 4828 nt long and encodes the 34.1-kda movement protein (nsm) and the 127.7-kda glycoprotein precursor (gn/gc). the s rna is 2949 nt long and encodes the 52.4-kda silencing suppressor protein (nss) and the 29.3-kda nucle ... | 2015 | 25427981 |
| trichoderma l-lysine-α-oxidase producer strain culture fluid inhibits impatiens necrotic spot virus. | a method for pcr diagnosis of impatiens necrotic spot virus is developed. concentrated culture fluid with active l-lysine-α-oxidase (0.54 u/ml) from trichoderma harzianum rifai fungus producer strain f-180 inhibits vitally hazardous impatiens necrotic spot phytovirus. | 2016 | 26742733 |
| development of reverse transcription thermostable helicase-dependent dna amplification for the detection of tomato spotted wilt virus. | a protocol for the reverse transcription-helicase-dependent amplification (rt-hda) of isothermal dna was developed for the detection of tomato spotted wilt virus (tswv). specific primers, which were based on the highly conserved region of the n gene sequence in tswv, were used for the amplification of virus's rna. the lod of rt-hda, reverse transcriptase-loop-mediated isothermal amplification (rt-lamp), and reverse transcriptase-polymerase chain reaction (rt-pcr) assays were conducted using 10-f ... | 2016 | 27538747 |
| development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to tomato spotted wilt virus. | tospoviruses, the plant-infecting genus in the family bunyaviridae, are thrips borne and cause severe agricultural losses worldwide. based on the serological relationships of the structural nucleocapsid protein (np), the current tospoviruses are divided into six serogroups. the use of np-antisera is convenient for virus detection, but it is insufficient to identify virus species grouped in a serogroup due to the serological cross-reaction. alternatively, virus species can be identified by the n ... | 2017 | 28081705 |
| an efficient and high fidelity method for amplification, cloning and sequencing of complete tospovirus genomic rna segments. | tospoviruses (genus tospovirus, family bunyaviridae) are responsible for major losses in an extensive range of crops worldwide. new species of these single-stranded, ambisense rna viruses regularly emerge and have been shown to maintain heterogeneous populations with individual isolates having quite variable biological and virulence characteristics. most tospovirus phylogenetic studies have focused on analysis of a single gene, most often the nucleocapsid protein gene. complete genomic rna segme ... | 2017 | 28082165 |
| development of a molecular assay for the general detection of tospoviruses and the distinction between tospoviral species. | a luminex xtag-based assay for plant-infecting tospoviruses was developed. the test enables the detection of tospoviruses in general and the differentiation of the four important member species of this genus: tomato spotted wilt virus, impatiens necrotic spot virus, the proposed 'capsicum chlorosis virus' and watermelon silver mottle virus. the generic tospovirus primers used in this method are also applicable for detection of tospoviruses by basic rt-pcr. we also describe an economic alternativ ... | 2017 | 28190200 |
| the nsm proteins of phylogenetically related tospoviruses trigger sw-5b-mediated resistance dissociated of their cell-to-cell movement function. | the cell-to-cell movement protein (nsm) of tomato spotted wilt virus (tswv) has been recently identified as the effector of the single dominant sw-5b resistance gene from tomato (solanum lycopersicum l.). although most tswv isolates shows a resistance-inducing (ri) phenotype, regular reports have appeared on the emergence of resistance-breaking (rb) isolates in tomato fields, and suggested a strong association with two point mutations (c118y and t120n) in the nsm protein. in this study the sw-5b ... | 2017 | 28754561 |