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frog lysozyme. i. its identification, occurrence as isozymes, and quantitative distribution in tissues of the leopard frog, rana pipiens.in the course of examining the etiology of the lucké renal adenocarcinoma of the frog, rana pipiens, it was found that organs of the normal adult contain bacteriolytic enzymes. these enzymes all satisfied the six criteria for the identification of lysozymes and at least eight forms were separable by polyacrylamide gel electrophoresis. their qualitative and quantitative distribution was organ-specific. all eight isozymes were found in normal kidney, while liver and spleen contained seven forms; s ...19764576
leopard syndrome. 19756088
[profuse lentigo, little leopard syndrome]. 197715225
in vitro measurement of the beta-adrenergic blocking properties of orf 12592, the 5-hydroxy analog of propranolol.orf 12592 caused concentration-dependent inhibition of isoproterenol stimulated adenylate cyclase activity in sarcolemma-enriched membrane preparations of guinea-pig myocardium. its potency was slightly less than that of d,l-propranolol. orf 12592 did not stimulate basal enzyme activity, suggesting it to be devoid of intrinsic sympathomimetic activity. it produced no marked inhibition of basal activity, nor did it inhibit sodium fluoride stimulated enzyme activity, indicating that the compound a ...197828704
central projection of optic tract from translocated eyes in the leopard frog (rana pipiens).in rana pipiens embryos, eye anlagen were moved to the evacuated ear position, where they continued to differentiate and sent their optic nerve fibers into the hindbrain. upon entering the medulla, the optic fibers turned caudally, penetrated the spinal cord, and traversed the dorsolateral white matter to the caudal end. we found this pattern of growth in every animal; the optic fibers did not enter the tecta. these results suggest the existence within the neural tube of a three-dimensional grad ...197550621
seasonal variation in the active transporting ability and in the membrane atpase activity of the frog intestinal epithelium.the active sugar and amino acid transport in the small intestine of the american leopard frog (rana pipiens) and a species of european frog (rana esculenta) decreases during the winter months. parallel with this the (na+, k+)-stimulated ("pump") atpase activity is markedly depressed. no seasonal changes are observed in the intestine of the tropical bullfrog (rana catesbeiana). it is assumed that the low pump-atpase activity is caused by the hibernation of the frogs living in moderate or subtropi ...2000139926
[multiple lentigines syndrome (leopard syndrome)]. 2011143001
segregation of rd-114 and fel-v-related sequences in crosses between domestic cat and leopard cat. 1975170535
virus-like particles in cystic mammary adenoma of a snow leopard.virus-like particles were observed in the giant cells of a mammary adenoma of a snow leopard kept in captivity. particles that measured 115 to 125 nm in diameter budded from the lamella of endoplasmic reticulum and were studded on their inner surfaces with dense granules (approximately 12 nm) that gave them their unique ultrastructural morphology. such particles were not observed extracellularly. type b or type c particles were not seen in the tumor tissue.1975171061
studies on the comparative pharmacology and selective toxicity of tricaine methanesulfonate: metabolism as a basis of the selective toxicity in poikilotherms.tricaine methanesulfonate, administered at a dose of 150 mg/kg i.p., produced a flaccid paralysis and loss of the righting reflex in a number of poikilothermic species including the frog. leopard frogs (rana pipiens) given 150 mg/kg i.p. regained the righting reflex at 113 +/- 28 (s.d.) minutes after injection. a similar dose administered i.p. to mice produced no apparent pharmacological response. the biological half-life (t1/2) of tricaine in frogs (r. pipiens) was about 70 minutes at temperatu ...1976185356
feline viral rhinotracheitis in a colony of clouded leopards. 1977203080
reduced prevalence of the lucké renal adenocarcinoma in populations of rana pipiens in minnesota.northern leopard frogs (rana pipiens) afflicted with the lucké renal adenocarcinoma virtually disappeared from minnesota in the autumn of 1977. frogs from four sites in minnesota counties (polk, otter tail, kandiyohi, and scott) with a previously high prevalence of lucké renal tumor were studied. in the past decade, prevalence averaged 4.2% in 29 collections (total, 1,870 frogs). no tumors were detected in 685 frogs autopsied in the autumn of 1977 by the method of previous studies. frog collecti ...1966224239
thermal acclimation in selected tissues of the leopard frog rana pipiens. 1975237657
the leopard that went to college. 1991257951
isolation of an actinomycete from a sea leopard (hydrurga leptonyx). 1977275688
[histogenetic studies on the teeth of leopard shark (triakis scyllia) (author's transl)]. 1978285188
a survey of blood and other tissue parasites of leopard frogs rana pipiens in the united states.in a survey of blood and other tissue parasites from 137 leopard frogs, rana pipiens complex, purchased from 13 commercial vendors in 8 states in the united states, trypanosoma pipientis was found in 2 r. p. berlandieri, toxoplasma ranae in 1 r. pipiens, isospora lieberkuehni in 1 leopard frog, haemogregarina magna in 44, lankesterella minima in 3, leptotheca ohlmacheri in 3 and microfilariae of foleyella sp. in 6. the report of i. lieberkuehni is presumably a new host record. haemogregarina tem ...1977300116
afferents to the optic tectum of the leopard frog: an hrp study.following unilateral hrp injections in the optic tectum of rana pipiens, hrp-positive cells were seen in three pretectal nuclei: bilaterally in the dorsal posterior nucleus; in the dorsal half of the ipsilateral posterior nucleus; and ipsilaterally in the large-called pretectal nucleus. hrp-positive cells were also seen ipsilaterally in the anterodorsal, posterodorsal and posteroventral tegmental fields, the nucleus isthmi, and the dorsal gray columns of the cervical spinal cord; bilaterally in ...1977300743
the germfree leopard frog (rana pipiens): preliminary report.eggs and sperm were aseptically collected from leopard frogs (rana pipiens) and passed into sterilized flexible film isolators. egg fertilization was accomplished, and germfree tadpoles were produced. the germfree tadpoles were active and free-swimming for 3 weeks, but only 6.2% survived to 4 weeks of age. none survived after the 4th week.1994302881
mate calling induced by electrical stimulation in freely moving leopard frogs, rana pipiens. 1977303606
frog lysozyme. v. isolation and some physical and immunochemical properties of lysozyme isozymes of the leopard frog, rana pipiens.frog lysozyme has been purified by sequential application of acid extraction, salt fractionation, cm-cellulose chromatography, heat treatment, and gel filtration. eight isozymes of purified lysozyme were found to be stable during prolonged storage. isozymes were separated by preparative polyacrylamide gel electrophoresis, ninety percent of the lytic activity of frog ovarian egg was represented by forms 7 and 8, the most highly charged isozymes. seventy-eight percent of frog liver lysozyme activi ...1977303690
effect of temperature on serum complement levels in the leopard frog, rana pipiens. 1977305862
leopard frog (rana pipiens) spleen lymphocyte responses to plant lectins: kinetics and carbohydrate inhibition. 2000305868
lens structural disorders in the leopard frog (rana pipiens) supplied to the laboratory from wild populations.examination of lenses of 48 leopard frogs, rana pipiens, from three commercial suppliers disclosed a high incidence of histologic aberrations. only four of 17 lenses that appeared totally clear under the dissecting microscope were found to be free of discernible abnormalities on histologic examination. deviations were most common in the area of elongation. because of these findings, the applicability of frog lenses for cellular investigations was questioned.1978305977
regeneration of ankylosed teeth in the adult frog premaxilla.the relationship between the ankylosed amphibian tooth and regeneration of a jaw segment was studied. a section of the premaxilla was removed in 95 young leopard frogs. subsequent would healing was observed at intervals of 0-180 days. the dental lamina formed new teeth and by 90 days, teeth in varying stages of development could be seen extending across the wound segment. teeth within the wound grew to normal size and shape and were replaced by their successors without support of underlying jaw ...1978306183
wavelength discrimination in the leopard frog: a reexamination.a reexamination of wavelength discrimination using a 2-choice paradigm and both chromatic-chromatic and chromatic-achromatic stimulus pairs has reaffirmed the positive tendency of rana pipiens to approach short wavelengths. in addition, a strong avoidance of long wavelengths was observed and an interaction of these two opposite response tendencies was demonstrated. thus, the percent choice frequency for a short-wavelength stimulus depends upon the stimulus with which it is paired. a positive pho ...1978310705
the effects of four arthropod diets on the body and organ weights of the leopard frog, rana pipiens, during vitellogenesis.wild-caught adult rana pipiens females were captured in midsummer and fed diets of crickets, flies sowbugs or wax moth larvae during a three-month period of active vitellogenesis. the cricket diet supported the most extensive body weight gain during this time and promoted a prolonged period of weight increase in an additional long-term study. synchronous growth of the oocytes occurred in all four groups, but the ovaries and oviducts of cricket-fed animals were significantly larger than those of ...1978312231
increased prevalence of mature females bearing pigmented oocytes from populations of minnesota rana pipiens.mature female leopard frogs, rana pipiens, collected from several sites in minnesota from 1967 through 1977, were examined for the presence of pigmented oocytes. an increase in the percent of mature females with pigmented oocytes was observed in the frogs captured in 1977 compared to those captured earlier. coincident with the increase in prevalence of females with pigmented oocytes, there was a decline in abundance of the frogs.1992312362
species differences in the intraretinal electroretinogram within the leopard frog complex. 1979312562
clarification of studies on the origin of thymic lymphocytes.the thesis that lymphocytes originate in situ by the direct transformation of epithelial cells within the thymic primordium in anurous frogs is untenable. on the contrary, in both the leopard frog and the african clawed toad, the lymphocytes that first appear in the embryonic thymus are derived from extrathymic lymphopoietic cells that invaded the developing organ. the exact source of origin of the invading lymphopoietic cells remains problematic.2000313974
activation of leopard frog (rana pipiens) spleen lymphocytes by concanavalin a and phytohemagglutinin. 1965315831
fine structure of leopard frog (rana pipiens) lymphocytes after in vitro transformation by phytohemagglutinin. 1979315891
transcriptionally active and inactive regions of nucleolar chromatin in amplified nucleoli of fully grown oocytes of hibernating frogs, rana pipiens (amphibia, anura). a quantitative electron microscopic study.chromatin structures contained in nuclei of full-grown oocytes of hibernating northern leopard frogs, rana pipiens, kept at 4 degrees c were examined by light and electron microscopy, with special emphasis on the morphology of spread nucleoli. light microscopic inspection of the in situ arrangement revealed that at this stage of oogenesis the numerous amplified nucleoli, together with the chromosomes, were included in a centrally located aggregate. characteristically, the nucleoli appeared spher ...1979316784
complete elimination of mitosis and dna synthesis in the lens of the hypophysectomized frog: effects on cell migration and fiber growth.three weeks after hypophysectomy mitosis and dna synthesis are absent in the lens of the leopard frog (rana iiens). by labeling germinative zone cells in their last dna synthetic period we were able to follow them in the absence of further proliferation. by this means it has been demonstrated that migration of epithelial cells to the equator of the lens is stopped. based on indirect measurements it seems that lens fiber formation also ceases. a correlation coefficient of 0.86 was computed betwee ...1998316785
specificity of carbohydrate inhibition on leopard frog (rana pipiens) erythrocyte agglutinating activity by concanavalin a and phytohemagglutinin.1. leopard frog (rana pipiens) erythrocytes are agglutinated by the plant lectins concanavalin a and phytohemagglutinin. 2. concanavalin a hemagglutination can be specifically inhibited by the sugars mannose, glucose, arabohexulose, alpha-methyl-d-mannopyranoside, n-acetyl-d-glucosamine, maltose, sucrose, trehalose and melezitose. 3. sugars inhibiting concanavalin a hemagglutination are structurally composed of alpha-d-glucopyranosyl, alpha-d-mannopyranosyl or beta-d-fructofuranosyl residues wit ...1979318545
tazonomy of toxoplasma.after reviewing reports of the hosts, structure and life cycle of toxoplasma, the genus is placed in the apicomplexan family eimeriidae and the folllowing 7 species are recognized: toxoplasma gondii (nicolle & manceaux) (type species) from about 200 species of mammals and birds, with oocysts in felids; toxoplasma alencari (da costa & pereira) from the frog leptodactylus ocellatus; toxoplasma brumpti coutelen from the iguana iguana tuberculata; toxoplasma colubri tibaldi from the snakes coluber m ...1977325203
a short-term culture method for the preparation of chromosome spreads from rana pipiens.chromosomes suitable for karyotyping were produced from short-term cultures of cells from embryonic northern leopard frogs, rana pipiens, at various growth stages. embryos were minced in a trypsin solution. the tissue fragments were subsequently cultured in an electrolytic solution containing fetal calf serum and colcemid. we found this technique to be highly reproducible. in contrast to the conventional squash technique we were able to obtain a greater number of metaphase spreads suitable for i ...1981327613
thyrotropin releasing hormone (trh) in pineal and hypothalamus of the frog: effect of season and illumination.the influence of photo-illumination and season on the pineal and hypothalamic content of trh in the leopard frog (rana pipiens) was studied. animals (4-6 in each group) were exposed to constant light or darkness for 72 h and then sacrificed. the pineal and hypothalamus from each frog were extracted for trh measurement by radioimmunoassay. the experiment was performed in spring, autumn, early winter and mid-winter. in early winter, mid-winter and spring the pineal content of trh ranged from 0.14- ...1977401486
[atypical pulmonary stenosis in certain polymalformative syndromes. prevalence of associated hypertrophique myocardiopathy].certain pulmonary stenoses differ markedly from the usual types of isolated valvular stenosis in their clinical features (maximal murmur situated lower down), their radiological signs (absence of a prominent median arc), the electrocardiograph (atypical electrical axis), the haemodynamic findings (usually a poor gradient), and especially the angiocardiography (valvular dysplasia, lack of a post-stenotic dilatation). they are encountered especially in the multiple malformations, as shown by this ...1977405948
purification of gonadotropins in the leopard frog (rana pipiens). 1977408226
[leopard syndrome].three cases of leopard-syndrome are presented. a review of literature is given. among the symptoms of the syndrome lentigines, ecg-abnormalities, pulmonal-stenosis, retardation of growth and deafness show the strongest penetrance and are most constantly found.1979483956
inheritance of spotting in the leopard danio.the inheritance of spotting pattern was investigated in the teleosts brachydanio nigrofasciatus (leopard danio) and b. frankei (spotted danio). the segregation patterns observed in the progenies from nine different matings indicate a single-factor inheritance of the leopard and spotted phenotypes with complete dominance of the leopard type. in view of these observations, it is suggested that the species status of b. frankei be reexamined.2006489959
[leopard syndrome and pituitary insufficiency]. 1979496534
premature cataracts associated with generalized lentigo.generalized lentigo (leopard syndrome) is an autosomal dominant trait characterized by lentigo, sensorineural deafness, retarded growth (below 25%), ocular hypertelorism, mandibular prognathism, pectus carinatum or excavatum, dorsal kyphosis, winging of the scapulae, valvular pulmonary artery stenosis, electrocardiographic conduction defects, and genitourinary defects. ocular evaluations of patients with generalized lentigo have revealed the appearance of multiple small white punctate and comma- ...1979545823
[lentiginosis profusa syndrome and the heart. leopard syndrome and hypertrophic obstructive cardiomyopathy]. 1977560516
[lentigino-cardiomyopathic syndrome (incomplete leopard syndrome) (author's transl)]. 1978630445
an endoscopic approach to a snow leopard. 1978648831
[leopard syndrom. four observations (author's transl)].four cases of leopard syndrom in a mother and in three children of hers are reported. the main clinical and electrocardiographic features characterizing the syndrome are exposed and analyzed in relation with phacomatosis and pathoetiologic mechanism.2006717925
notoedric mange in a litter of snow leopards. 1978738955
inclusion body hepatitis in a black panther (panthera pardus pardus). 1978742261
rate of listeria monocytogenes shedding from frogs.leopard frogs (rana pipiens) were orally inoculated with listeria monocytogenes. most listeriae were shed 3-6 days after inoculation. the evidence indicated that the listeriae did not multiply in frogs and were unable to become established in the gastrointestinal tract.1975806711
fluorescent cytochemistry of calid and algid normal and lucké tumor-bearing kidneys.fluorescent cytochemical techniques, chiefly end-group methods, have been employed in the characterization of cells in calid and algid normal and lucke tumor-bearing kidneys of the leopard frog, rana pipiens. cytoplasmic rna and glycogen distribution reflect the known patterns of general metabolic activity of the respective cells in question. protein sulfhydryl distribution is similar in normal distal tubules and tumors while both differ significantly from proximal tubules. viral inclusions in n ...1975814775
purification and properties of reptilian and amphibian growth hormones.highly purified growth hormone was isolated from the pituitaries of two reptilian species, the snapping turtle and the sea turtle, and two amphibian species, the bullfrog and the leopard frog. characterization studies were performed with these growth hormones in comparison with mammalian and avian growth hormones. great similarities among these species were found in chromatographic behavior, ve/vo ratios (2.0) on gel filtration, disc electrophoretic patterns, terminal amino acid residues and imm ...1976821743
fine structure of leydig cells in crabeater, leopard and ross seals.ultrastructural study of the leydig cells of nonbreeding crabeater, leopard and ross seals showed that three types of cells could be distinguished. type i cells possessed the cytological features typical of steroid-secreting cells. type ii cells exhibited various features of degeneration, e.g. accumulation of large amounts of lipofuscin granules (residual bodies), lipid droplets, secondary lysosomes, rectangular crystalloids, and previously undescribed 'peculiar bodies'. these cellular inclusion ...1977833788
partial characterization of the bile salt-dependent triacylglycerol lipase from the leopard shark pancreas.leopard shark triacylglycerol lipase has been characterized as a crude pancreatic preparation. the enzyme demonstrated an absolute requirement for trihydroxy bile salts for activity with natural bile salts of the shark giving a 4-fold greater stimulation of activity than pure sodium taurocholate. bile salts also protected the enzyme from apparent inactivation by p-chloromercuribenzoate and trypsin treatment. the shark lipase demonstrated a temperature optimum of 36 degrees c and was rapidly inac ...1977836861
fine structure of seminiferous tubules in antarctic seals.the fine structure of seminiferous tubules from 5 crabeater, 2 leopard and 2 ross seals showed that during the nonbreeding season the tubules were essentially similar in possessing spermatogenic and sertoli cells. however, the tubules of leopard and ross seals had more primary and secondary spermatocytes and spermatids than the crabeater seals. in general, the tubules were devoid of spermatozoa. the spermatids showed stages of maturation such as golgi phase of acrosome formation, acrosomal cap f ...1977844074
congenital lentiginosis.because lentiginosis may be associated with multiple somatic anomalies (as in the "leopard syndrome"), its recognition is important clinically. a case of lentiginosis detected in the neonatal period, without accompanying defects, is reported. the significance of histologic confirmation and the importance of searching for other abnormalities are discussed.1977862424
[cardiovascular changes in the leopard syndrome]. 1977866252
tiletamine and zolazepam for immobilization of wild lions and leopards.a 1:1 mixture of tiletamine and zolazepam was used for the immobilization of lions on 26 occasions and of leopards on 22 occasions. there was a significant (p less than 0.001) positive linear relationship between duration of anesthesia and dosage for all animals in which the duration was recorded (n = 36). this response could be divided into 4 separate regressions according to species and sex; lions being more susceptible to the drug than leopards and males more so than females. when dosage was ...1977924863
mortality in red-sided garter snakes, thamnophis sirtalis parietalis, due to larval nematode, eustrongylides sp.garter snakes, thamnophis sirtalis parietalis, that had been fed wild-caught banded killifish, fundulus diaphanus, and laboratory-bred leopard frogs, rana pipiens, were infected by larval nematode, eustrongylides sp; 7 of the 25 snakes died. signs of infection in 20 snakes included subcutaneous "lumps," violent contortions, and bloody exudate, apparently from the nares. nemotodes were collected at necropsy from beneath the skin, in the lungs, in the body cavity, and along the spinal column. nema ...1976940307
giant and "granular melanosomes" in leopard syndrome: an ultrastructural study.electron microscopy of lentigines was performed to study the pigmentation abnormality in two children with leopard syndrome. giant melanosomes similar to those seen in café-au-lait spots of neurofibromatosis and nevus spilus were found in a lentigine from one of our cases. our results show that "spherical granular melanosomes" described in neurofibromatosis, are lysosomal-like structures associated with the development of complex melanin granules. our study also demonstrates that immature melano ...19761018060
lead insult to the dentition in the leopard. 19751073183
the influence of thyroxine and acclimation temperature on glycogen reserves of the frog rana pipiens.leopard frogs were given daily injections of na-l-thyroxine (2.0 mug/10 g body weight) for seven days, and control animals were given daily injections of the vehicle. during the period of treatment, half of the frogs were acclimated, in darkness and without food, at a constant temperature of 15 degrees c, whereas the remainder of the animals were acclimated at 25 degrees c. at the end of the seventh day, the frogs were killed by decapitation, and the glycogen concentration in samples of liver ti ...19751079235
cytophysiological basis of disruptive pigmentary patterns in the leopard frog rana pipiens. ii. wild type and mutant cell-specific patterns.a new pattern index, ip, is introduced and used to compare patterns of wild type, burnsi, and kandiyohi chromatophores in the leopard frog, rana pipiens. wild type chromatophores are hyperdispersed over distances within cellular contact, and it is concluded that this hyperdispersion results from contact-mediated negative interactions. the hyperdispersion is less strong in spot cells than interspot, and extends over larger areas in burnsi than in wild type epidermis. over areas greater than chrom ...19751080207
thymus: central role in the immune system of the frog.in the leopard frog (rana pipiens), the thymus profoundly influences immunologic development by providing virtually all the progenitor lymphocytic cells for the bone marrow and other peripheral lymphoid organs. the thymus houses a self-perpetuating population of lymphopoietic cells that originally self-differentiated in that gland.19751081275
frog lysozyme. ii. isozymes of lysozyme during the larval development of the leopard frog, rana pipiens. 20011083817
serum thyroxine concentrations in the pacific hagfish and lamprey and in the leopard frog. 19761084847
correlation between blood level of androgens and sexual behavior in male leopard frogs, rana pipiens. 19761084850
evidence for rhythms of mitotic activity in normal and adenocarcinoma cells of the renal tubules of rana pipiens.kidneys were studied from 1,111 leopard frogs (rana pipiens) after exposures to a 12-hour light-12-hour dark environment. circadian mitotic rhythms were observed in cells of normal and spontaneous adenocarcinomatous proximal convoluted tubules from these animals. student's t-tests verified the existence of two distinct cell populations, normal and adenocarcinomatous, in the proximal tubules. both the mitotic variations and the t-test values were significant with p values of 0.05 or less. mean mi ...20051087350
thymus: primary site of lymphopoiesis.the thymus of the leopard frog is the primary, if not the sole, source of progenitor lymphocytic cells that normally colonize the spleen. following embryonal parabiosis, a high level of cell chimerism exists in the thymus glands of each member of the parabiotic pair. evidently, there can be an appreciable amount of cross-circulation of thymic cells between thymus glands.20051087542
alternate sites of lymphopoiesis in the amphibian embryo.the presumptive ventral blood islands were reciprocally transplanted between chromosomally labelled 72-hour-old leopard frog embryos. at 10 days of development, the differentiating epithelial thymus glands were surgically removed. following metamorphosis, the spleen and bone marrow from juvenile frogs were analysed cytophotometrically. during normal ontogeny, the vast majority of peripheral lymphocytes are derived from the thymus, not the ventral blood islands. in the absence of the thymus, the ...20051087543
lentiginosis profusa. iii: aesthetic and sanguineous aspects.lentiginosis profusa syndrome and progressive cardiomyopathic lentiginosis are preferable terms to "leopard syndrome". considerable cosmetic improvement followed facial dermabrasion and light electrodesiccation of lentigines on the exposed surfaces. hematologic evaluation did not reveal hematopoietic defect in the patient studied.20051087902
some anatomical aspects of the cardiovascular system of antarctic seals and their possible functional significance in diving.the hearts and ascending aortae of 11 weddell seals, leptonychotes weddelli, three adult crabeater seals, lobodon carcinophagus, two adult ross seals, ommatophoca rossi, and one adult leopard seal, hydrurga leptonyx, were examined for comparison with terrestrial forms. the weddell seal specimens were from animals ranging in age from midterm in fetal development to mature adults. all specimens were collected in 1971, 1972, and 1973, from mcmurdo sound and the ross sea, antarctica. the phocid hear ...19751111424
[cardiac arrhythmias in vector cardiograms in the "leopard" syndrome (author's transl)].disturbances of cardial conduction in the leopard syndrome are described. in this syndrome disturbances of cardial conduction are better demonstrated and diagnosed in the vcg than in the ecg.19751172162
high levels of pancreatic nonspecific lipase in rattlesnake and leopard shark.hydrolysis of synthetic triglycerides by rattlesnake and leopard shark pancreatic enzymes revealed striking differences in specificity, depending on the presence or absence of sodium taurocholate. without added sodium taurocholate the classical specificity of pancreatic lipase was expressed. rattlesnake enzymes, in the presence of sodium taurocholate, attacked the unsaturated oleic acid in the 2-position of racemic glycerol-1-palmitate-2-oleate-3-stearate nearly twice as fast as either outside s ...19751177670
observations on naturally acquired hepatozoonosis of wild carnivores and dogs in the republic of south africa.hepatozoonosis was studied in hyaenas, lions, jackals, cheetahs and one leopard in the kruger national park and compared with the condition seen in dogs in the republic of south africa. hepatozoon schizonts were found in the wild carnivores. the genesis of microschizonts was followed and is illustrated. the schizonts were sometimes very plentiful in the lung, myocardium and skeletal muscle, and were also encountered in the spleen, liver and lymph nodes. gametocytes were present in leucocytes. th ...19751221330
pentastomiasis (armillifer and linguatula sp.) infestations of wild animals in the kruger national park.armillifer armillatus, linguatula serrata and l. nuttalli have each been isolated from nine different mammalian species in the kruger national park: lion, panthera leo; leopard, p. pardus; buffalo, syncerus caffer: blue wildebeest, connechaetes taurinus; giraffe, girraffa camelopardalis; kudu, tragelophus strepsiceros; waterbuck, kobus ellipsyprymnus; tsessebe, damaliscus iunatus and impala, aepyceros melampus. successful transmission of l. serrata from the lion to a domestic ox and impala is re ...19751223283
cardio-cutaneous syndrome (the "leopard" syndrome). review of the literature and a new family.numerous reports of familial cardiac disorders associated with abnormalities of cutaneous pigmentation have appeared in the literature. in some of these there have been other somatic malformations including retardation of growth and anomalous development of the genitalia. in this paper we review the literature and describe another family with multiple lentigines occurring in six members out of eight examined. they were reported to occur in 10 other members not seen by us. the trait showed an aut ...19761261064
flavobacterium indologenes infection in leopard frogs.an investigation of an epidemic of infectious disease in a frog (rana pipiens) colony was conducted. six of 40 frogs in a continuous (once through) water flow housing system had weight loss, swollen abdomen, corneal edema, uveitis, subcutaneous edema, petechial hemorrhage, incoordination, and respiratory distress. the frogs had lesions consistent with bacterial septicemia. a gram-negative, nonfermenting bacillus, flavobacterium indologenes (flavobacterium sp biovar iib), was isolated in pure cul ...19921293124
[leopard spot chorioretinopathy. initial manifestation of recurrent acute lymphocytic leukemia].an 11-year-old girl with a history of acute lymphocytic leukemia of the central nervous system had attained complete remission for almost 3 1/2 years after combination chemotherapy and radiotherapy when she developed iritis and chorioretinopathy of the right eye. neither an anterior chamber tap nor a diagnostic vitrectomy revealed leukemic cells. both nonspecific anti-inflammatory therapy and antiviral treatment were unsuccessful. finally, lymphoblasts were detected in the cerebrospinal fluid an ...19921304225
human onchocerciasis in the lower jos plateau, central nigeria: the prevalence, geographical distribution and epidemiology in akwanga and lafia local government areas.an investigation of human infection with onchocerca volvulus and the resulting clinical disease was carried out for the nigerian national onchocerciasis control programme between july and august 1989 [corrected]. the survey covered 10.6% of the rural population in 41 savanna villages of central nigeria. of the 8451 self-selected individuals examined, 900 (10.6%) had skin microfilariae (mf). there were differences between villages in both endemicity and intensity of infection, but in general the ...19921304706
the inactivating potassium currents of hair cells isolated from the crista ampullaris of the frog.1. a-type outward currents were studied in sensory hair cells isolated from the semicircular canals (scc) of the leopard frog (rana pipiens) with whole-cell voltage- and current-clamping techniques. 2. there appear to be two classes of a-type outward-conducting potassium channels based on steady-state, kinetic, pharmacological parameters, and reversal potential. 3. the two classes of a-type currents differ in their steady-state inactivation properties as well as in the kinetics of inactivation. ...19921336044
retrovirus infections in non-domestic felids: serological studies and attempts to isolate a lentivirus.an african lioness from the zoo of zurich had to be euthanized because of an inoperable tumor. the serum tested negative for feline leukemia virus (felv) p27 antigen by enzyme-linked immunosorbent assay (elisa) but was strongly positive for feline immunodeficiency virus (fiv) antibodies by elisa and western blot. when her only offspring and mate were tested for fiv, high antibody titers to fiv were also found in their serum. lymphocytes were prepared from these two lions on different occasions a ...19921337398
mycoplasma simbae sp. nov., mycoplasma leopharyngis sp. nov., and mycoplasma leocaptivus sp. nov., isolated from lions.mycoplasmas isolated from the throats of lions were shown to belong to three serotypes, all of which were serologically distinct from the previously recognized mycoplasma and acholeplasma spp. eight mycoplasma colonies were cloned, including one from a leopard (strain lp), and were examined in detail for morphology, growth, and biochemical characteristics. the strains had the following properties: guanine-plus-cytosine contents of 37 mol% (strain lxt [t = type strain]), 28 mol% (strain ll2t), an ...19921390104
directional dependence of auditory sensitivity and frequency selectivity in the leopard frog.direction-dependent changes in frequency selectivity were documented in three populations of neurons in the auditory periphery of the leopard frog: low-, mid-, and high-frequency sensitive fibers. these changes were most pronounced in the mid-frequency (720-1199 hz) sensitive fibers which exhibited positive shifts in characteristic frequencies (cfs) and concomitant narrowed bandwidths from posterior speaker presentations. maximum sensitivities were observed for ipsilateral speaker presentations ...19921401540
digital image analysis of shark gills: modeling of oxygen transfer in the domain of time.digital radiographic imaging of blood circulation through leopard shark gills establishes a secondary lamellar transit time of 6.5 s. this duration, combined with estimates of cardiac output and hemoglobin-oxygen affinity, permits novel modeling of gill oxygen transfer in the time domain. the temporal model allows assessment of factors contributing to previously noted discrepancies between physiological and morphometric branchial oxygen conductance estimates. lamellar transit time for shark bloo ...19921415783
accumulation of membrane-bound melanosomes occurs in langerhans cells of patients with the leopard syndrome.the langerhans cells in the lentigines of four patients with the leopard syndrome contained large membrane bound accumulations of melanin granules. giant melanosomes were only seen in two patients. the patients had no immune-based symptoms relating to their lentigines. the leopard syndrome, also known as multiple lentigines syndrome, progressive cardiomyopathic lentiginosis, lentiginosis profusa syndrome and the cardiocutaneous syndrome, refers to an inherited abnormality of the skin, often asso ...19921424250
hibernation alters the frog's immune system.the lymphomyeloid organs and blood leukocyte populations of the leopard frog, rana pipiens, undergo conspicuous changes during hibernation at 4 degrees c. within the blood, spleen, thymus, jugular bodies, and bone marrow there was a progressive loss of hemopoietic populations resulting in a marked lymphocyte depletion. termination of the 135-day hibernation period resulted in the restoration of all hemopoietic elements in the blood and lymphomyeloid organs within 30 days. frogs subjected to expe ...19921424717
neural correlates of frog calling: production by two semi-independent generators.the anterior preoptic nuclei of the isolated brainstem of male, northern leopard frogs (rana p. pipiens) were stimulated electrically and neural correlates of mating calling recorded from the rhombencephalic mating calling pattern generator. lesions of discrete areas of the brainstem showed that the mating calling generator is separable into two generators, the pretrigeminal nucleus and the classical pulmonary respiration generator (which is approximately co-extensive with the motor nuclei ix-x) ...19921449644
silhouettes elicit alarm calls from captive vervet monkeys (cercopithecus aethiops).in part 1, we analyzed alarm calls produced by captive vervets (cercopithecus aethiops) in response to naturally occurring stimuli. females and juveniles regularly alarm called to airplanes, birds, and barking dogs. juvenile females accounted for 60% of these alarm calls. in part 2, we isolated several monkeys from the colony and presented them with life-sized silhouettes of a leopard, snake, eagle, baboon, vervet, and goose. adult monkeys alarm called more than did juveniles. alarm calls were e ...19921451417
redeployment of trigeminal motor axons during metamorphosis.as a consequence of the degeneration and replacement of the jaw muscle fibers in the leopard frog, rana pipiens, trigeminal motoneurons innervate different targets before and after metamorphosis. this investigation examined the morphological correlates of the reassignment of trigeminal motoneurons during the initial phases of myofiber turnover. specifically, silver-cholinesterase histochemistry and electron microscopy were used to 1) identify the fate of motor axons within the neuromuscular junc ...19921484115
is there a seasonal variation in mucus transport and nutrient absorption in the leopard frog?we postulated that as a hibernating species, frogs might have variable demands for nutrients at different seasons of the year and that this must be reflected in seasonal variations of physiologic processes related to nutrient transport and absorption. we examined the rate of mucus transport on the ciliated palate and the movement of nutrients across the intestinal lumen of leopard frogs, rana pipiens. mucus transport on the frog palate was strongly influenced by season, with maximal transport oc ...19921498713
directionality of phase locking in auditory nerve fibers of the leopard frog rana pipiens pipiens.a dorsal approach to the eighth nerve and free-field stimulation were used to investigate the effect of sound direction and intensity on phase locking in auditory nerve fibers of the leopard frog rana pipiens pipiens. tuning curves of 75 auditory neurons were analyzed (fig. 2). amphibian papillar neurons, but not basilar papillar neurons, exhibit significant phase locking to short tone bursts at the characteristic frequency (cf), the degree of phase locking (vector strength) decreasing with the ...19921507157
carnivora: the primary structure of the major hemoglobin component from adult european lynx (lynx lynx, felidae).the complete primary structure of the major hemoglobin component from the adult european lynx (lynx lynx) is presented. presence of two hemoglobin components and three chains, beta a, beta b, and alpha, identified by gel electrophoresis. the purification of the globin chains achieved by ion-exchange chromatography. the globin chains were digested with trypsin. the peptide generated were purified by reversed-phase hplc. sequencing of the native chains up to 42 cycles and of the tryptic peptides w ...19921515033
onchocerciasis prevalence in previously known foci in western uganda: results from a preliminary survey in kabarole district.a prevalence survey of onchoceriasis was done in kabarole district, uganda, in 1990. the objective was to determine the prevalence of onchocerciasis among communities living in previously known foci. a total number of 1186 persons was included in the study. the infection rate among the communities studied ranged from 0% to 67%. the most frequent clinical signs and symptoms of onchocerciasis were: pruritus (80%), rough skin (46%), skin rash (30%), leopard skin (6%) and sclerosing keratitis (7%). ...19921519030
innervation of the amphibian and basilar papillae in the leopard frog: reconstructions of single labeled fibers.amphibians have two auditory organs specialized for reception of airborne sounds: the amphibian papilla and the basilar papilla. in this report we examine the morphology of the ganglion cells and the afferent innervation of the sensory epithelium in both auditory organs of the leopard frog, rana pipiens pipiens. extracellular injections of either biocytin or horseradish peroxidase (hrp) were made into the viii nerve; they labeled ganglion cells, their axons, and their terminal fibers within the ...19921522248
metabolic fate of lactate after vigorous activity in the leopard frog, rana pipiens.although the ability of isolated frog muscle to synthesize glycogen from lactate has long been known, it has never been demonstrated that this metabolic activity occurs in the intact frog. our results clearly indicate that lactate glycogenesis in frog muscle occurs to a significant extent in vivo. during recovery from strenuous exercise, most of the lactate accumulated by frogs seems to be recycled into muscle glycogen because the lactate that disappears during recovery could account nearly stoi ...19921539733
crystal structure of the unique parvalbumin component from muscle of the leopard shark (triakis semifasciata). the first x-ray study of an alpha-parvalbumin.the three-dimensional structure of parvalbumin from leopard shark (triakis semifasciata) with 109 amino acid residues (alpha-series) is described at 1.54 a resolution. crystals were grown at 20 degrees c from 2.9 m-potassium/sodium phosphate solutions at ph 5.6. the space group is p3(1)21 and unit cell dimensions are a = b = 32.12 a and c = 149.0 a. the structure has been solved by the molecular replacement method using pike 4.10 parvalbumin as a model. the final structure refinement resulted in ...19921542115
isolation of p multocida f-3, 4 from a stillborn snow leopard. 19921542981
temporal coding in the frog auditory midbrain: the influence of duration and rise-fall time on the processing of complex amplitude-modulated stimuli.1. single-unit recordings were made in the auditory midbrain, the torus semicircularis (ts) of the northern leopard frog, to independently characterize the processing of different temporal attributes (signal duration, rise-fall time, and rate of amplitude modulation) of natural sounds and to investigate how these temporal variables interact to produce the observed responses to complex amplitude-modulated (am) signals. response functions, on the basis of mean spike count, were derived and categor ...19921552312
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