Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| isolation and characterization of hemorrhagic, myonecrotic and edema-inducing toxins from bothrops insularis (jararaca ilhoa) snake venom. | bothrops insularis snake venom was fractionated by gel filtration on sephadex g-150 followed by ion-exchange chromatography on sp-sephadex c-25. two active fractions were purified to homogeneity: (1) siii-spi, approximate mol. wt 32,000 and n-terminal amino acid residue val. this fraction showed esterase activity on tame, edema-inducing activity on the rat hind paw and contractile activity on the isolated guinea pig ileum. the latter two activities were antagonized by benadryl plus methysergide; ... | 1990 | 2343462 |
| primary structure and biological activity of bradykinin potentiating peptides from bothrops insularis snake venom. | several bradykinin potentiating peptides (bpps) were isolated from the venom of the brazilian arboricole snake bothrops insularis by gel filtration on sephadex g-150-120, followed by sequencial high-voltage paper electrophoreses at ph 3.5, 6.5, and 2.1. the bpps were assayed by their ability to potentiate the contractile activity, on the isolated guinea pig ileum, and the hypotensive activity, on anesthetized rats, of bradykinin. eight bpps, containing 3-13 amino acid residues, were sequenced an ... | 1990 | 2386615 |
| isolation and characterization of a thrombin-like enzyme from the venom of the snake bothrops insularis (jararaca ilhoa). | a thrombin-like enzyme (i-sii-r) from the venom of the brazilian snake bothrops insularis (jararaca ilhoa) was purified to homogeneity by gel filtration on sephadex g-150 followed by precipitation of contaminating proteins with half-saturated nacl and two further gel filtrations under the same conditions. i-sii-r showed specific activities of 820 clotting units/mg protein and 530 tame units/mg protein (a 23-fold purification) and a single precipitation band against antibothropic horse antiserum ... | 1987 | 3433290 |
| effect of bothrops insularis venom on the mouse and chick nerve-muscle preparation. | the effects of bothrops insularis venom were examined in vivo in mice and chicks and in vitro using the mouse phrenic nerve diaphragm and chick biventer cervicis muscle preparations. incubation of the indirectly or directly stimulated mouse preparation with b. insularis venom (20-80 micrograms/ml) produced an initial increase in twitch tension followed by irreversible blockade. with direct stimulation in the presence of d-tubocurarine, no increase in twitch tension was observed prior to the onse ... | 1993 | 8303718 |
| an unusual presynaptic action of bothrops insularis snake venom mediated by phospholipase a2 fraction. | a phospholipase a2-containing fraction was isolated from the venom of bothrops insularis by a combination of gel filtration on sephadex g-150 and ion exchange chromatography on deae-sephadex. peak iv of the latter chromatography containing all of the phospholipase a2 (pla2) activity, was assayed on isolated neuromuscular preparations. in the mouse phrenic nerve-diaphragm incubated in tyrode at 37 degrees c, the pla2 fraction produced an initial increase in the twitch tension and in the frequency ... | 1998 | 9723831 |
| histopathological changes in avian kidney caused by bothrops insularis (jararaca ilh a) venom and a phospholipase a2-containing fraction. | the histopathological changes induced in avian kidney by the intramuscular injection of bothrops insularis (jararaca ilh a) venom and its phospholipase a2 (pla2)-containing fraction were examined. acute experiments (3 h and 24 h) with b. insularis crude venom (20 microg and 80 microg) or its pla2-contaning fraction (10 microg and 40 microg) resulted in significant structural damage to the kidneys of 5-12-day-old chicks. histopathological analysis indicated that the venom and its fraction acted o ... | 2001 | 11193194 |
| molecular cloning and expression of a functional snake venom vascular endothelium growth factor (vegf) from the bothrops insularis pit viper. a new member of the vegf family of proteins. | during the generation of abundant expressed sequence tags from the viperidae snake bothrops insularis venom glands, we identified for the first time a cdna coding for a putative vascular endothelial growth factor-like (vegf-like) protein. the deduced primary sequence, after complete sequencing of the longest snake venom vegf (svvegf) cdna, displayed similarity with vertebrate vegfs and with the hypotensive factor from vipera aspis venom. its cdna was subcloned, expressed in escherichia coli with ... | 2001 | 11517227 |
| fluorometric assay using naphthylamide substrates for assessing novel venom peptidase activities. | in the present study we examined the feasibility of using the fluorometry of naphthylamine derivatives for revealing peptidase activities in venoms of the snakes bothrops jararaca, bothrops alternatus, bothrops atrox, bothrops moojeni, bothrops insularis, crotalus durissus terrificus and bitis arietans, of the scorpions tityus serrulatus and tityus bahiensis, and of the spiders phoneutria nigriventer and loxosceles intermedia. neutral aminopeptidase (apn) and prolyl-dipeptidyl aminopeptidase iv ... | 2002 | 12419513 |
| a survey of gene expression and diversity in the venom glands of the pitviper snake bothrops insularis through the generation of expressed sequence tags (ests). | in order to produce a global panorama of the transcriptional activity of snake venom glands and to correlate with its venom composition, we constructed a dna complementary to rna library from the venom glands of the viperidae snake bothrops insularis for the generation of expressed sequence tags (ests). sequences from 610 independent clones were grouped in 297 clusters, revealing the putative identification of 210 distinct gene products. toxin sequences correspond to 56% of all transcripts (85 c ... | 2002 | 12459276 |
| cloning and expression of calglandulin, a new ef-hand protein from the venom glands of bothrops insularis snake in e. coli. | the ef-hand protein family is comprised of many proteins with conserved ca(2+)-binding motifs with important biological roles in intracellular communication. during the generation of expressed sequence tags (ests) from the venom glands of the viperidae snake bothrops insularis, we identified a cdna coding for a putative ca(2+) binding protein with four ef-hand motifs, named here calglandulin. the deduced amino acid sequence displayed the greatest sequence similarity with calmodulin (59%), follow ... | 2003 | 12758151 |
| somatic pairing, endomitosis and chromosome aberrations in snakes (viperidae and colubridae). | the positioning of macrochromosomes of bothrops jararaca and bothrops insularis (viperidae) was studied in undistorted radial metaphases of uncultured cells (spermatogonia and oogonia) not subjected to spindle inhibitors. colchicinized metaphases from uncultured (spleen and intestine) and cultured tissues (blood) were also analyzed. we report two antagonic non-random chromosome arrangements in untreated premeiotic cells: the parallel configuration with homologue chromosomes associated side by si ... | 2003 | 12947479 |
| pharmacological characterization of mouse hind paw oedema induced by bothrops insularis (jararaca ilhoa) snake venom. | bothrops snake venoms produce marked local effects, including oedema, haemorrhage and necrosis. the ability of bothrops insularis venom to induce oedema in mice was investigated. venom was injected into hind paws and the change in volume over time was measured by plethysmometry. b. insularis venom (0.01-2.5 microg/paw) induced paw oedema which, at high doses (>/=0.5 microg/paw), was accompanied by haemorrhage. the peak oedematogenic response occurred 3 h after venom injection with all doses and ... | 2003 | 14529733 |
| pharmacological evidence for a presynaptic action of venoms from bothrops insularis (jararaca ilhoa) and bothrops neuwiedi (jararaca pintada). | whereas the presynaptic action of crotalus durissus terrificus venom is well-established, bothrops venoms have historically been considered to have only postsynaptic and muscular effects. however, some studies have also suggested a presynaptic action for these venoms. in this work, we used chick biventer cervicis preparations to compare the presynaptic actions of two bothrops venoms (b. insularis and b. neuwiedi) with that of c. d. terrificus venom. at 10 microg/ml, all venoms produced irreversi ... | 2004 | 15109884 |
| cloning, characterization, and structural analysis of a c-type lectin from bothrops insularis (bil) venom. | lectins are carbohydrate-binding molecules that mediate a variety of biological processes. in this work, we identify and characterize a lectin from bothrops insularis venom, with respect to its biochemical properties and theoretical structure. initially, from a venom gland cdna library, we cloned and sequenced a cdna encoding a protein with high identity to snake venom lectins. a lectin molecule was purified to homogeneity from the venom by affinity column and gel filtration. this protein named ... | 2004 | 15519291 |
| cloning and characterization of human caglp gene encoding a novel ef-hand protein. | the ef-hand proteins, containing conserved ca2+ binding motifs, play important roles in many biological processes. through data mining, a novel human gene, caglp (calglandulin-like protein) was predicted and subsequently isolated from human skeleton muscle. the open reading frame of caglp is 543 bp in length, coding a putative ca2+ binding protein with four ef-hand motifs. the deduced amino acid sequence of caglp displays high similarity with bothrops insularis snake protein calglandulin (80%). ... | 2004 | 15621662 |
| insularinase a, a prothrombin activator from bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains. | the first low-molecular-mass metalloprotease presenting prothrombin activating activity was purified from bothrops insularis venom and named insularinase a. it is a single-chain protease with a molecular mass of 22 639 da. cdna sequence analysis revealed that the disintegrin domain of the precursor protein is post-translationally processed, producing the mature insularinase a. analysis of its deduced amino acid sequence showed a high similarity with several fibrin(ogen)olytic metalloproteases an ... | 2005 | 16006246 |
| purification and biological effects of c-type lectin isolated from bothrops insularis venom. | bothrops insularis is a snake from queimada grande island, which is an island located about 20 miles away from the southeastern coast of brazil. compared to other brazilian species of bothrops, the toxinology of b. insularis is still poorly understood. its c-type lectin is involved in several biological processes including anticoagulant and platelet-modulating activities. we purified the c-type lectin (bilec) from bothrops insularis venom and investigated its effect in the isolated kidney. bilec ... | 2006 | 16730365 |
| phylogeography of the bothrops jararaca complex (serpentes: viperidae): past fragmentation and island colonization in the brazilian atlantic forest. | the brazilian atlantic forest is one of the world's major biodiversity hotspots and is threatened by a severe habitat loss. yet little is known about the processes that originated its remarkable richness of endemic species. here we present results of a large-scale survey of the genetic variation at the mitochondrial cytochrome b gene of the pitviper, jararaca lancehead (bothrops jararaca), and two closely related insular species (bothrops insularis and bothrops alcatraz), endemic of this region. ... | 2006 | 17054497 |
| purification, sequencing and structural analysis of two acidic phospholipases a2 from the venom of bothrops insularis (jararaca ilhoa). | bothrops snake venoms contain a variety of phospholipases (pla(2)), some of which are myotoxic. in this work, we used reverse-phase hplc and mass spectrometry to purify and sequence two pla(2) from the venom of bothrops insularis. the two enzymes, designated here as bintx-i and bintx-ii, were acidic (pi 5.05 and 4.49) asp49 pla(2), with molecular masses of 13,975 and 13,788, respectively. the amino acid sequence and molecular mass of bintx-i were identical to those of a pla(2) previously isolate ... | 2006 | 17140721 |
| purification and biological activity of the thrombin-like substance isolated from bothrops insularis venom. | the venom of bothrops insularis snake, known in brazil as jararaca ilhoa, contains a variety of proteolytic enzymes such as a thrombin-like substance that is responsible for various pharmacological effects. b. insularis venom chromatography profile showed an elution of seven main fractions. the thrombin-like activity was detected in fractions i and iii, the latter being subjected to two other chromatographic procedures, so to say deae and hi trap benzamidine. the purity degree of this fraction w ... | 2007 | 17161857 |
| enzymatic and immunochemical characterization of bothrops insularis venom and its neutralization by polyspecific bothrops antivenom. | herein we compared the biological activities of bothrops insularis and bothrops jararaca venoms as well as their neutralization by polyspecific bothrops antivenom (pba). on account of that, we investigated their antigenic cross-reactivity and the neutralization of lethal, myotoxic and defibrinating activities by polyspecific and species-specific antivenoms. silver-stained sds-page gels evidenced many common bands particularly above 47 kda between b. jararaca and b. insularis venoms. however, som ... | 2007 | 17382362 |
| variability in expression of bothrops insularis snake venom proteases: an ontogenetic approach. | bothrops insularis is a threatened snake endemic to queimada grande island, southern coast of são paulo, brazil, and the occurrence of sexual abnormalities in males, females and intersexes (females with functional ovaries and rudimentary hemipenis) has been reported in this population. the aim of this study was to identify ontogenetic shifts in protease expression of offspring of captive-bred b. insularis. three neonates from a single litter were maintained at the facilities of laboratory of her ... | 2007 | 17398162 |
| purification and renal effects of phospholipase a(2) isolated from bothrops insularis venom. | bothrops insularis venom contains a variety of substances presumably responsible for several pharmacological effects. we investigated the biochemical and biological effects of phospholipase a(2) protein isolated from b. insularis venom and the chromatographic profile showed 7 main fractions and the main phospholipase a(2) (pla(2)) enzymatic activity was detected in fractions iv and v. fraction iv was submitted to a new chromatographic procedure on ion exchange chromatography, which allowed the e ... | 2008 | 17953979 |
| purification and biological effects of l-amino acid oxidase isolated from bothrops insularis venom. | bothrops insularis is a snake from ilha da queimada grande, an island located about 20 miles away from the southeastern coast of brazil. compared with other brazilian species of bothrops, the toxinology of b. insularis is still poorly understood, and so far, no fraction from this venom with amino acid oxidase activity had been isolated or its biological activity tested. we investigated the biochemical and biological effects of one l-amino acid oxidase enzyme isolated from b. insularis snake veno ... | 2008 | 17983639 |
| identification and characterization of a new member of snake venom thrombin inhibitors from bothrops insularis using a proteomic approach. | snake venom c-type lectin-like proteins (clps) are ubiquitously found in viperidae snake venoms and differ from the c-type lectins as they display different biological activities but no carbohydrate-binding activity. previous analysis of the transcriptome obtained from the bothrops insularis venom gland showed the presence of two clusters homologous to bothrojaracin (bjc) chains alpha and beta. in an effort to identify a new bjc-like molecule, we used an approach associated with proteomic techno ... | 2008 | 18221976 |
| bothrops insularis venomics: a proteomic analysis supported by transcriptomic-generated sequence data. | a joint transcriptomic and proteomic approach employing two-dimensional electrophoresis, liquid chromatography and mass spectrometry was carried out to identify peptides and proteins expressed by the venom gland of the snake bothrops insularis, an endemic species of queimada grande island, brazil. four protein families were mainly represented in processed spots, namely metalloproteinase, serine proteinase, phospholipase a(2) and lectin. other represented families were growth factors, the develop ... | 2009 | 19211044 |
| crotalid snake venom subproteomes unraveled by the antiophidic protein dm43. | snake venoms are mixtures of proteins and peptides with different biological activities, many of which are very toxic. several animals, including the opossum didelphis aurita, are resistant to snake venoms due to the presence of neutralizing factors in their blood. an antihemorrhagic protein named dm43 was isolated from opossum serum. it inhibits snake venom metalloproteinases through noncovalent complex formation with these enzymes. in this study, we have used dm43 and proteomic techniques to e ... | 2009 | 19267469 |
| transcriptome analysis of the amazonian viper bothrops atrox venom gland using expressed sequence tags (ests). | bothrops atrox is a highly dangerous pit viper in the brazilian amazon region. we produced a global catalogue of gene transcripts to identify the main toxin and other protein families present in the b. atrox venom gland. we prepared a directional cdna library, from which a set of 610 high quality expressed sequence tags (ests) were generated by bioinformatics processing. our data indicated a predominance of transcripts encoding mainly metalloproteinases (59% of the toxins). the expression patter ... | 2009 | 19708221 |
| "insularin, a disintegrin from bothrops insularis venom: inhibition of platelet aggregation and endothelial cell adhesion by the native and recombinant gst-insularin proteins". | insularin (ins) was obtained from bothrops insularis venom by reversed-phase high-performance liquid chromatography using a c(18) column and characterized as a disintegrin by peptide mass fingerprint and inhibition of adp-induced platelet aggregation. a cdna coding for p-ii a metalloproteinase/disintegrin was cloned from a cdna library from b. insularis venom glands. the deduced protein sequence possesses 73 amino acid residues, including the n-terminal, internal peptides of native insularin, th ... | 2011 | 21073888 |
| bj-pi2, a non-hemorrhagic metalloproteinase from bothrops jararaca snake venom. | envenoming by bothrops jararaca can result in local pain, edema, hemorrhage and necrosis, partially mediated by snake venom metalloproteinases (svmps). here, we describe the characterization of bj-pi2, a p-i class svmp from b. jararaca venom, and its local tissue actions. | 2012 | 22867987 |
| population dynamics of the critically endangered golden lancehead pitviper, bothrops insularis: stability or decline? | little is known about vital rates of snakes generally because of the difficulty in collecting data. here we used a robust design mark-recapture model to estimate survival, behavioral effects on capture probability, temporary emigration, abundance and test the hypothesis of population decline in the golden lancehead pitviper, bothrops insularis, an endemic and critically endangered species from southeastern brazil. we collected data at irregular intervals over ten occasions from 2002 to 2010. sur ... | 2014 | 24755842 |
| combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two bothrops jararaca populations from geographic isolated regions within the brazilian atlantic rainforest. | bothrops jararaca is a slender and semi-arboreal medically relevant pit viper species endemic to tropical and subtropical forests in southern brazil, paraguay, and northern argentina (misiones). within its geographic range, it is often abundant and is an important cause of snakebite. although no subspecies are currently recognized, geographic analyses have revealed the existence of two well-supported b. jararaca clades that diverged during the pliocene ~3.8mya and currently display a southeaster ... | 2016 | 25968638 |
| annual changes in seminal variables of golden lanchead pitvipers (bothrops insularis) maintained in captivity. | bothrops insularis is an endemic and critically endangered snake with an estimated population of 2000 individuals restricted to queimada grande island, in southeastern brazil. brazilian researchers established a captive breeding program for the species that includes the application of assisted reproductive technologies. the present study, therefore, aimed to evaluate semen samples from captive b. insularis throughout the year to ascertain seasonal differences in semen traits as well as correlati ... | 2015 | 26559333 |
| isolation and characterization of bradykinin potentiating peptides from agkistrodon bilineatus venom. | snake venom is a source of many pharmacologically important molecules. agkistrodon bilineatus commonly known as cantil, is spread over central america particularly in mexico and costa rica. from the venom of agkistrodon bilineatus we have isolated and characterised six hypotensive peptides, and two bradykinin inhibitor peptides. the ic-50 value of four synthesized peptides was studied, towards angiotensin converting enzyme, in order to study the structure-function relationship of these peptides. | 2016 | 26770072 |
| toxin fused with sumo tag: a new expression vector strategy to obtain recombinant venom toxins with easy tag removal inside the bacteria. | many animal toxins may target the same molecules that need to be controlled in certain pathologies; therefore, some toxins have led to the formulation of drugs that are presently used, and many other drugs are still under development. nevertheless, collecting sufficient toxins from the original source might be a limiting factor in studying their biological activities. thus, molecular biology techniques have been applied in order to obtain large amounts of recombinant toxins into escherichia coli ... | 2017 | 28264436 |
| molecular identification and phylogenetic analysis of bothrops insularis bacterial and fungal microbiota. | bothrops insularis, known as the golden lancehead snake, has its natural habitat restricted to queimada grande island on the southern coast of brazil. this culture-dependent study aimed to identify microorganisms obtained from the mouth, eyes, and cloaca of this species. swabs from 20 snakes were collected for fungal and bacterial isolation. dna was extracted from all samples, and identification was performed by amplifying the its1-5.8s-its2 regions and the 16s rdna gene, respectively. all strai ... | 2018 | 29319420 |
| evaluation of kim-1 as an early biomarker of snakebite-induced aki in mice. | acute kidney injury (aki) is one of the most important complications of bothropic poisoning and its early identification remains challenging. the nephrotoxicity of bothrops insularis venom (binsv) was previously described by our research group. in this study, we continued to evaluate the effect of binsv on kidney function in mice and llc-mk2 proximal tubule cells, evaluating kim-1 protein as an early aki biomarker. male swiss mice were inoculated with binsv intramuscularly and observed for 24 h ... | 2018 | 29909065 |