Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| thynnascaris rhacodes sp. n. (nematoda: ascaridoidea) in fishes from the israeli mediterranean coast. | thynnascaris rhacodes sp. n. infects pelates quadrilineatus, solea vulgaris, boops boops, lithognathus mormyrus, obleida melanura, diplodus vulgaris, d. sargus, and sparus auratus from the eastern mediterranean sea. unique in that the cuticle on the female tail is wrinkled, the ascaridoid is further characterized by possessing 27 to 35 precloacal papillae, a body length of 11 to 85 mm, similar spicules 3 to 6% of body length, a length-ratio of intestinal cecum to ventricular appendage of 1 : 1 t ... | 2003 | 570370 |
| [study of the larval stages of monogenea of fishes (author's transl)]. | the ciliate cells and the oncomiracidial chetotaxy of the following species are described: among the polyopisthocotylea : gastrocotyle trachurii van bneden and hesse, 1863, gastrocotylidae, parasite of trachurus trachurus (l.); cyclocotyla bellones (otto, 1821), diclidophoradae, living on the cymothoides isopods of sparidae : boops boops (l.) and spicara maena (l.) ; mazocraes sp., mazocraeidae, parasite of sardinella maderensis (lowe, 1841). among the monopisthocotylea : ligophorus vanbenedeni ... | 1978 | 754614 |
| immunocytochemistry of somatotrophs, gonadotrophs, prolactin and adrenocorticotropin cells in larval sea bream (sparus auratus) pituitaries. | the chronological appearance of endocrine cells in the pituitary of sea-bream (sparus auratus) larvae was studied using antisera against salmon prolactin, trout growth hormone, salmon gonadotropin and n-terminal human adrenocorticotropin. the larval pituitary (1-12 days after hatching) was oval in shape and was composed of a dense mass of cells with few neurohypophysial fibres. by 60 days after hatching it began to resemble the adult and was divisible into a distinct rostral pars distalis contai ... | 1992 | 1330314 |
| developmental expression of the growth hormone gene in the gilthead sea bream sparus aurata. | expression of growth hormone (gh) gene during early stages of larval development of the teleost sparus aurata was determined by northern blot analysis. poly(a+) rna was prepared from a pool of larvae collected on different days after hatching. when hybridized to sparus aurata gh cdna, gh specific mrna was first seen on day 6 post-hatching. in contrast, the levels of beta-actin mrna, which was used to normalize for rna amounts, were already high on the day of hatching. our results suggest that ex ... | 1992 | 1359982 |
| somatostatin 14- and somatostatin 25-like peptides in pancreatic endocrine cells of sparus aurata (teleost): a light and electron microscopic immunocytochemical study. | an immunocytochemical investigation demonstrates the presence of somatostatin (sst) 14- and salmon somatostatin (ssst) 25-like peptides in two populations of somatostatin (d) cells in the islets of gilthead sea bream (sparus aurata). both cell types were identified by their differing immunoreactivities to the somatostatin antisera used. d1 cells in the islet periphery between glucagon cells showed ssst 25-like immunoreactivity and contained large moderate to low electron-dense granules. d2 cells ... | 1992 | 1383077 |
| extraction of lipids and lipophilic antioxidants from fish tissues: a comparison among different methods. | 1. lipids, phospholipids and lipid soluble antioxidants were extracted from sparus auratus liver and white muscle by three different methods and the yields obtained were compared. 2. none of the three procedures can recover the above components with the same efficiency. 3. for comparison the methods were also applied to rat liver homogenates. 4. the choice of the extraction procedures depends on the tissue investigated and on specific research requirements. | 1992 | 1582177 |
| light and electron microscopic immunocytochemical demonstration of the coexistence of somatostatin 14- and somatostatin 25-like peptides in endocrine cells of the stomach of sparus aurata (teleost). | an immunofluorescence double-staining method colocalized somatostatin 14 (sst 14)- and somatostatin 25 (sst 25)-like immunoreactivities in endocrine cells located in the depth of gastric folds and upper part of the stomach glands of sparus aurata (gilthead sea bream). an immunogold method identified somatostatin-like peptides in the secretory granules of the previously described type iv endocrine cells. appropriate preabsorption controls demonstrated two different granule populations with somato ... | 1991 | 1685719 |
| fish plasma lipoproteins--comparative observations in serranides and sparides. | 1. diet, time from last feeding, temperature, season and sexual stage are some of the factors influencing the lipoprotein pattern. 2. keeping these factors constant species-specific differences observed among lipoprotein patterns of sparus aurata, puntazzo puntazzo, diplodus sargus, diplodus vulgaris and dicentrarchus labrax are discussed. 3. feeding habits and therefore lipid absorption and the rate of lipoprotein maturation process are the factors determining the observed differences. | 1991 | 1764905 |
| morphology and ultrastructure of cryptobia eilatica n. sp. (bodonidae: kinetoplastida), an ectoparasite from the gills of marine fish. | a marine kinetoplastid flagellate, cryptobia eilatica n. sp., is described from the gills of cultured gilt-head sea bream sparus aurata l. and wild black-spot sea bream diplodus noct (valenciennes) in the red sea. the trophozoite is elongated and lacks a contractile vacuole and undulating membrane. the body averages 13.5 x 4.1 microns, anterior flagellum 9.7 microns and free portion of recurrent flagellum 15.2 microns. the ultrastructural features of the species exhibit great similarity to vario ... | 1990 | 2086780 |
| fructose 2,6-bisphosphate in liver of sparus aurata: influence of nutritional state. | 1. fructose 2,6-bisphosphate (fru-2,6-p2) has been measured in liver and muscle of gilthead sea bream fish, sparus aurata. 2. the fru-2,6-p2 levels in liver depend on the diet given to the fish: in fish fed a high carbohydrate diet, the fru-2,6-p2 levels are higher than any one previously reported. these changes are associated with differences in the phosphofructokinase 2 activity. 3. fru-2,6-p2 levels has also been measured in liver of sparus aurata after different fasting periods. in starved f ... | 1990 | 2142067 |
| degradation of gonadotropin-releasing hormones in the gilthead seabream, sparus aurata. i. cleavage of native salmon gnrh and mammalian lhrh in the pituitary. | the pattern and kinetics of degradation of native salmon gonadotropin-releasing hormone (sgnrh) and mammalian leuteinizing hormone-releasing hormone (lhrh) by pituitary bound enzymes were studied in the gilthead seabream, sparus aurata. sgnrh and lhrh were incubated for different periods of time with membrane or cytosolic fractions of pituitary homogenates. at the end of the incubation, the degradation mixture was fractionated on reverse-phase high-pressure liquid chromatography. the degradation ... | 1990 | 2202610 |
| an immunocytochemical and ultrastructural study of endocrine cells in the gut of a teleost fish, sparus auratus l. | endocrine cells in the gut of sparus auratus l. (gilt-head sea bream) have been demonstrated by immunocytochemical and electron microscopic techniques. cells showing somatostatin and gastrin-like immunoreactivity were found in the depth of the gastric folds and in the upper part of the stomach glands while substance p immunoreactive cells were present between the upper epithelial cells of the gastric folds. cells showing gastrin, substance p, pancreatic polypeptide, cholecystokinin, and met-enke ... | 1986 | 2433180 |
| histochemistry of glycoconjugates in the gallbladder epithelium of ten animal species. | a battery of seven lectins and several conventional mucin histochemical techniques were used to identify the epithelial mucins of the gallbladder of ten species: man, rabbit (oryctolagus cuniculus, mammalia), hamster (mesocricetus auratus, mammalia), chicken (gallus gallus, bird), sparrow (passer domesticus, bird), moorish gecko (tarentola mauritanica, reptilia), ladder snake (elaphe scalaris, reptilia), lake frog (rana perezi, amphibia), natterjack toad (bufo calamita, amphibia) and gilthead se ... | 1989 | 2722572 |
| immunocytochemical and ultrastructural characterization of the cell types in the adenohypophysis of sparus aurata l. (teleost). | the structure and immunocytochemistry of the adenohypophysis of sexually mature male specimens of sparus aurata (gilthead sea bream) were studied. the adenohypophysis was composed of rostral pars distalis (rpd), proximal pars distalis (ppd), and pars intermedia (pi). in the rpd the prolactin cells were organized into follicles which occupied a very reduced area as corresponds to that in saltwater fishes; the corticotropic cells were surrounding the pars nervosa branches and the prolactin follicl ... | 1988 | 2848744 |
| growth hormone increases plasma levels of insulin-like growth factor (igf-i) in a teleost, the gilthead seabream (sparus aurata). | a heterologous radioimmunoassay (ria) was applied for the determination of immunoreactive (ir)--insulin-like growth factor (igf-i) in a teleost, the gilthead seabream (sparus aurata). serial dilutions of the fish plasma gave a linear curve when added to constant amounts of 125i-labelled human igf-i(53-70) and antiserum prepared against this fragment. the ria was used to study the effect of gh on plasma levels of ir-igf-i in s. aurata. a single injection of human recombinant gh (1 micrograms/g) r ... | 1989 | 2926294 |
| immunocytochemical and ultrastructural characterization of coexistence of pancreatic polypeptide and glucagon-like immunoreactivity in the pancreatic endocrine cells of sparus auratus l. (teleostei). | coexistence of pancreatic polypeptide (pp)- and glucagon-like immunoreactivity was demonstrated in the pancreatic endocrine cells of the teleost fish sparus auratus. an immunofluorescence double-staining method revealed coexistence of glucagon- and pp-like immunoreactivity in endocrine cells of small and intermediate islets. in contrast to small islets, the intermediate ones also contained a variable number of glucagon-immunoreactive cells next to cells having both immunoreactivities. coexistenc ... | 2011 | 3131188 |
| cyclic histological changes in the pituitary gland of sparus aurata in correlation to the gonadal cycle. | 2012 | 3410381 | |
| an immunocytochemical and ultrastructural study of the endocrine pancreas of sparus auratus l. (teleostei). | the pancreatic endocrine cells of sparus auratus (gilthead sea bream) are concentrated in two or three principal islets, or brockmann bodies, and numerous smaller islets embedded in the exocrine tissue. insulin-, glucagon-, somatostatin-, and pancreatic polypeptide (pp)-immunoreactive cells were identified in all pancreatic islets of s. auratus using an indirect immunocytochemical (pap) method. insulin-immunoreactive cells were found in the central region of the islets. glucagon-immunoreactive c ... | 2012 | 3549431 |
| evidence for a direct action of gh on haemopoietic cells of a marine fish, the gilthead sea bream (sparus aurata). | receptors for gh were characterized in the head kidney of gilthead sea bream (sparus aurata), using radioiodinated and biotinylated ligands. the specific binding of radiolabelled recombinant gilthead sea bream gh (rsbgh) to head kidney membrane preparations was dependent on membrane concentration. salmon prolactin, salmon gonadotrophin and carp gonadotrophin did not compete for 125i-labelled rsbgh-binding sites. unlabelled rsbgh competitively displaced 125i-labelled rsbgh bound to head kidney me ... | 1995 | 7595141 |
| cloning and characterisation of a fish aldolase b gene. | a full length cdna clone representing an aldolase mrna was isolated from a sea bream (sparus aurata) liver cdna library. sequencing of this clone revealed it to encode a 364 amino acid protein with 74% amino acid identity to human aldolase b and slightly lower similarity to human aldolase a and c. in view of the sequence data and of northern blot analysis showing strong expression of a 1.6 kb transcript in liver it was concluded that the cloned gene represents aldolase b. this clone represents t ... | 1995 | 7632737 |
| the muscle isoform of 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase of the teleost sparus aurata: relationship with the liver isoform. | the liver isoform of 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase of the teleost fish sparaus aurata has several characteristics similar to the skeletal muscle isoform of mammals. in order to ascertain the relation between muscle and liver isoforms in teleost, 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was purified from skeletal muscle of s. aurata. the muscle isozyme is composed of subunits with a molecular weight of 54 kda, is bifunctional, and has an activity ratio kinase to ... | 1995 | 7646054 |
| the use of recombinant gilthead sea bream (sparus aurata) growth hormone for radioiodination and standard preparation in radioimmunoassay. | a gilthead sea bream growth hormone (sbgh) obtained by cloning and expression of sbgh cdna was used to develop a sensitive and specific radioimmunoassay (ria). iodination of recombinant sbgh (rsbgh) was performed by the classical chloramine-t method. specific antiserum, raised in rabbits, was added in a final dilution of 1/36,000. the minimum detectable dose was 30 pg, and the midrange of the assay (ed50) was 275 pg. intra- and inter-assay coefficients of variation (cv) were 3.3 and 5.8% at ed50 ... | 1995 | 7669108 |
| natural hemolytic and bactericidal activities of sea bream sparus aurata serum are effected by the alternative complement pathway. | sea bream serum displayed bactericidal and hemolytic activities. these activities were depleted when serum was incubated with different activators of the alternative complement pathway (acp). ethylenediaminetetraacetic acid (edta) inhibited both the hemolytic and bactericidal activities, while ethyleneglycol-bis (b-aminoethyl ether)-n, n, n'-tetraacetic acid (egta) was not inhibitory. an antibody against the putative third component of sea bream component (c3) was produced. it was observed by im ... | 1995 | 7676614 |
| a histochemical and immunohistochemical study of digestive enzymes and hormones during the larval development of the sea bream, sparus aurata l. | the distribution of different hydrolytic enzymes and the localization of the hormones which regulate glucose metabolism during development of the digestive tract of the sea bream, sparus aurata l., were studied. the yolk sac contains trypsin, glucose-6-phosphatase, atpases and acid and alkaline phosphatase activities. positive insulin, glucagon and somatostatin cells were observed in the pancreas and in the lumen of the intestinal tract during endogenous feeding. from hatching until 3 days later ... | 1993 | 7689548 |
| molecular cloning and characterization of a novel gonadotropin-releasing hormone from the gilthead seabream (sparus aurata). | a novel form of gonadotropin-releasing hormone (gnrh) had been recently isolated and characterized from the gilthead seabream (sparus aurata). this novel form, designated sbgnrh, was suggested to be the endogenous stimulator of gonadotropin release. this paper reports on the isolation and characterization of a 360-bp cdna encoding the complete sbgnrh precursor. this precursor is composed of a 25 amino acid leader sequence, the biologically active sbgnrh, the cleavage site (gly-lys-arg), and a 57 ... | 1995 | 7749463 |
| mitochondrial dna variation in an experimental stock of gilthead sea bream (sparus aurata). | we present a restriction site map for the mitochondrial dna of the gilthead sea bream and the consensus sequence for a region of the mitochondrial cytochrome b gene. restriction site and nucleotide sequence analysis was used to obtain individual mitotype profiles in a broodstock used for genetic studies. eight mitotypes were detected among 33 animals assayed. in combination with hypervariable nuclear markers, this variation can be used to identify the parents of any offspring in mass matings and ... | 1995 | 7773328 |
| somatolactin, a novel pituitary protein: isolation and characterization from sparus aurata. | the complete amino acid sequence of somatolactin, a new pituitary protein belonging to the growth hormone/prolactin family, from the gilthead sea bream sparus aurata has been determined. somatolactin was isolated from the pituitary by alkaline extraction, gel filtration on a sephadex g-100 column, and reversed-phase high-performance liquid chromatography (rphplc) on a tsk gel ods-120t column. the purified protein was confirmed to be somatolactin by immunoblotting using chum salmon somatolactin a ... | 1995 | 7773329 |
| gonadotropin-releasing activities of the three native forms of gonadotropin-releasing hormone present in the brain of gilthead seabream, sparus aurata. | three forms of gonadotropin-releasing hormone (gnrh) have been recently identified in the brain of gilthead seabream (sparus aurata): salmon gnrh (sgnrh), chicken gnrh-ii (cgnrh-ii), and a novel form, ser8-mammalian gnrh, named seabream gnrh (sbgnrh). sbgnrh is the most abundant form in the pituitaries of sexually mature seabream during the spawning season. the present study investigated the gonadotropin-releasing activities of the three native forms of gnrh found in seabream brains, as well as ... | 1995 | 7789744 |
| isolation of growth hormone and in vitro translation of mrna isolated from pituitaries of the gilthead sea bream sparus aurata. | growth hormone (gh) polypeptide was purified from pituitary glands of the gilthead sea bream (sparus aurata) by a two-step procedure involving gel filtration on sephadex g-100 and reverse-phase high-performance liquid chromatography (rphplc). at each stage of purification, fractions were monitored by sds-polyacrylamide gel electrophoresis (sds-page) and by immunoblotting using anti-bonito gh antiserum. the molecular weight of the sea bream gh was estimated by sds-page to be 21 kda when electroph ... | 1994 | 7821767 |
| cloning, expression, and characterization of a recombinant gilthead seabream growth hormone. | cdna clones coding for the gilthead seabream (sparus aurata) growth hormone (sbgh) were isolated from a pituitary expression library using a flounder cdna probe. the nucleotide sequence of a gh cdna clone containing an insert of 896 nucleotides was determined. the cdna encoded a polypeptide of 204 amino acids including a signal peptide of 17 amino acids and contained a 5' and a 3' untranslated region of 48 and 233 nucleotides, respectively. the mrna determined by northern blot was approximately ... | 1994 | 7851717 |
| metabolic activation of carcinogenic aromatic amines by fish exposed to environmental pollutants. | activation of arylamines to mutagenic metabolites by hepatic s9 fractions has been evaluated as a biomaker of fish exposure to pollutants, using gilthead seabream (sparus aurata), a valuable fish species from the spanish south atlantic littoral, as model organism. to obtain maximal sensitivity to the mutagenic action of aromatic amines, a strain of salmonella typhimurium overproducing o-acetyltransferase was used. fish were treated with aroclor 1254, pesticides (malathion and dieldrin), or coppe ... | 1995 | 7875126 |
| ration size and protein intake affect circulating growth hormone concentration, hepatic growth hormone binding and plasma insulin-like growth factor-i immunoreactivity in a marine teleost, the gilthead sea bream (sparus aurata). | the nutritional regulation of the growth hormone liver axis has been studied in gilthead sea bream (sparus aurata). in a first study, fingerling fish were fed three experimental diets with varying proportions of protein (34, 45 and 55%). a 60% decrease in plasma growth hormone concentration was observed with the increase of specific growth rates and dietary protein levels. an opposite response was observed in hepatic growth hormone-binding sites and plasma insulin-like growth factor-i immunoreac ... | 1995 | 7876930 |
| non-specific cytotoxic response against tumor target cells mediated by leucocytes from seawater teleosts, sparus aurata and dicentrarchus labrax: an ultrastructural study. | the in vitro cytotoxic reaction of leucocytes from the seawater teleosts gilthead seabream (sparus aurata) and sea bass (dicentrarchus labrax) against tumor target cells was studied by transmission and scanning electron microscopy. head-kidney, blood or peritoneal exudate leucocytes were incubated with hela or b16 melanoma cells. after incubation, conjugates of leucocytes (effectors) and tumor cells (targets) were observed. both the effector-target cell binding and target cell lysis steps of the ... | 1994 | 7880589 |
| in vivo metabolism of [1-14c]linolenic acid (18:3(n-3)) and [1-14c]eicosapentaenoic acid (20:5(n-3)) in a marine fish: time-course of the desaturation/elongation pathway. | the metabolism (via the desaturation/elongation pathways) of [1-14c]18:3(n-3) and [1-14c]20:5(n-3) in a marine fish, gilthead sea bream (sparus aurata l.), were investigated over 8 days to determine the time-courses for the production of delta 6 and delta 5-desaturase products and 22:6(n-3). fish were starved for 1 week prior to, and during, the period of the experiment. the recovery of radioactivity from [1-14c]20:5(n-3) in tissue lipids exceeded that of [1-14c]18:3(n-3) at all time points. the ... | 1994 | 7908832 |
| promutagen activation by fish liver as a biomarker of littoral pollution. | metabolic activation of known promutagens by liver s9 fractions of mugil sp. (grey mullet) from two zones of the south atlantic spanish littoral was determined and related to their pollution levels. sediments from the putative contaminated area contained high concentrations of pahs, pcbs and pesticides, and animals from the polluted site exhibited higher concentrations of metals than those from the reference area. hepatic s9 fractions of mullets from the polluted site showed 5.1-, 18.6- and 42.8 ... | 1994 | 7925325 |
| ultrastructure of nonspecific cytotoxic cells in teleosts. i. effector-target cell binding in a marine and a freshwater species (seabream: sparus aurata l., and carp: cyprinus carpio l.). | fish cytotoxic effectors form a cell population whose ultrastructure and properties of conjugation with target cells have not been completely established. we report the ultrastructure of the nonspecific cytotoxic cells in a seawater teleost (sparus aurata l.) and compare it to a freshwater species (cyprinus carpio l.). | 1994 | 7978369 |
| ovarian melanotropic peptides and adaptation in two teleostean species: sparus aurata l. and dicentrarchus labrax l. | the ovarian tissue of dicentrarchus labrax and sparus aurata displays two immunoreactive peaks that correspond to the elution time of human des-acetyl alpha-msh [acth(1-13)-amide] and human alpha-msh. in view of the close identity between the primary structure of fish and human alpha-msh, these data demonstrate that two msh-related peptides are present both in sea bream and sea bass ovary. alpha-msh-like immunoreactivity was found within both granulosa and thecal layers of mature follicles, as w ... | 1994 | 7984515 |
| three forms of gonadotropin-releasing hormone characterized from brains of one species. | most vertebrate species have more than one form of gonadotropin-releasing hormone (gnrh) in their brains, but it is not clear whether each form has a distinct function. we report that sea bream (sparus aurata) brains have three forms of gnrh, one of which is described herein and is called sea bream gnrh (sbgnrh). the primary structures of two forms were determined by edman degradation and mass spectral analysis. the amino acid sequence of sbgnrh is pglu-his-trp-ser-tyr-gly-leu-ser-pro-gly-nh2. t ... | 1994 | 7991588 |
| perinotochordal connective sheet of gilthead sea bream larvae (sparus aurata, l.) affected by axial malformations: an histochemical and immunocytochemical study. | spinal malformations in adult teleosts occur under natural conditions and, more frequently, in culture exploitations. skeletal deformities are linked with dysfunctions in collagen metabolism. we studied axial deviations appearing in early larval stages of cultured sea bream (sparus aurata, l.). | 1994 | 7992891 |
| insulin binding and receptor tyrosine kinase activity in skeletal muscle of carnivorous and omnivorous fish. | we characterized the insulin receptors in skeletal muscle from several fish species with different nutritional preferences: brown trout (salmo trutta fario), gilthead sea bream (sparus aurata), tilapia (tilapia mossambica), and carp (cyprinus carpio), semipurified by affinity chromatography (wheat germ agglutinin-agarose). total specific binding and number of receptors per unit weight of piscine white skeletal muscle were lower than those values found in mammalian skeletal muscle. the same param ... | 1994 | 8024051 |
| in vivo effects of the anesthetic, benzocaine, on liver microsomal cytochrome p450 and mixed-function oxidase activities of gilthead seabream (sparus aurata). | gilthead seabreams were exposed to benzocaine, 4-aminobenzoic acid ethyl ester, 57 mg/l in sea water for 3 min, daily, for 2 or 3 consecutive days. the fish were killed 20 hr after the last treatment. benzocaine treatment for 2 or 3 days resulted in 57% and 67% inhibition of liver microsomal aniline 4-hydroxylase and ethylmorphine n-demethylase activities, respectively. the total cytochrome p450 content of fish liver microsomes was unaltered following the 2-day benzocaine treatment. however, add ... | 1994 | 8061946 |
| effects of in vivo benzo(a)pyrene treatment on liver microsomal mixed-function oxidase activities of gilthead seabream (sparus aurata). | benzo(a)pyrene [b(a)p] treatment of gilthead seabream, 25 mg/kg, i.p. for 5 consecutive days, did not cause any significant changes in ethylmorphine n-demethylase and aniline 4-hydroxylase activities of liver microsomes. the same treatment did not alter the liver microsomal cytochrome b5 content, nadh-cytochrome b5 reductase and nadph-cytochrome p450 reductase activities. however, benzo(a)pyrene treatment caused a 2-3-fold increase in 7-ethoxyresorufin o-deethylase (7-erod) activity of gilthead ... | 1994 | 8061947 |
| skeletal deformities of the gilthead sea bream (sparus aurata, l.): study of the subcommissural organ (sco) and reissner's fiber (rf). | a high incidence of lordotic curvatures has been detected in commercial cultures of sparus aurata. we have studied juvenile and adult lordotic specimens to elucidate whether the subcommissural organ and its secretion, the reissner's fiber, play any role in the development of this syndrome. animals were x-radiographed and then the brain and spinal cord dissected out and processed for light microscopy. adult lordotic fishes had a well developed swim-bladder whereas juvenile did not. the central ca ... | 1994 | 8085662 |
| 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase in liver of the teleost sparus aurata. | 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was purified from the liver of the teleost fish sparus aurata and the enzymatic activities were characterized kinetically. both activities copurify, being dimers of relative molecular mass of 98 kda with subunits of m(r) 54 kda. although both specific activities are in the range of mammalian liver isozymes, the kmfru 6-p of teleost 6-phosphofructo 2-kinase is 3 times that in rat liver. the s. aurata 6-phosphofructo 2-kinase is inhibited by adp ... | 1994 | 8109976 |
| developmental regulation of growth hormone binding in the gilthead sea bream, sparus aurata. | the occurrence of growth hormone (gh) binding was investigated in the gilthead sea bream larva, sparus aurata, using recombinant trout ghii (rtgh) as a tracer. gh binding was detected in all the specimens examined (5, 17, 27 and 40 days after hatching). at 40 days after hatching, we observed a concentration of gh binding in the head region. specific binding was dependent on protein membrane concentration and scatchard plots showed a single class of high affinity and low capacity binding sites. s ... | 1994 | 8193580 |
| parathyroid hormone-related protein is a factor in normal fish pituitary. | using antibodies to the amino-terminal region of human parathyroid hormone-related protein (pthrp) we have demonstrated pthrp immunoreactivity in pituitaries and plasma of the sea bream (sparus aurata). pituitary cells at two distinct locations contained immunodetectable pthrp; an anterior group in the rostral pars distalis which also contained immunoreactive thyroid stimulating hormone (tsh), and a posterior group lying at the border of the pars intermedia and proximal pars distalis between cel ... | 1993 | 8282170 |
| influence of environmental salinity on prolactin and corticotropic cells in the gilthead sea bream (sparus aurata l.). | the influence of the environmental salinity on the prolactin (prl) and corticotropic (acth) cells of the rostral pars distalis of the adenohypophysis of the gilthead sea bream (sparus aurata) adapted to sea water (sw, 980 mosm/kg) and brackish water (bw, 200 mosm/kg) has been studied by immunocytochemical, morphometric, and electron-microscopic techniques. prolactin (prl) cells of fish adapted to bw occupied a greater hypophysial volume (about 24% of the total hypophysial volume in bw, 10% in sw ... | 1993 | 8391498 |
| [effect of environmental salinity on the melanotropic cells of the gilthead bream (sparus aurata l.)]. | the influence of the environmental salinity on the msh cells of the pars intermedia in the euryhaline teleost sparus aurata has been investigated. control animals stayed in sea water (39/1000 salinity), and experimental fish in brackish water (7/1000 salinity) for two months. for light microscopy, pituitaries were fixed with bouin fluid and embedded in paraffin. for electron microscopy they were fixed with karvnosky and embedded in araldite. sections were stained with histochemical procedures an ... | 1993 | 8397430 |
| amino-acid sequence of bone gla protein from the african clawed toad xenopus laevis and the fish sparus aurata. | as an initial step in the analysis of bone gla protein (bgp; osteocalcin) function in lower vertebrates, we have developed a simple and rapid method for the isolation of bgp from bone and have applied this to the isolation of bgp from the african clawed toad xenopus laevis and the fish sparus aurata. we have also determined the complete amino-acid sequence of sparus and xenopus bgp, including the identification of the sites of y-carboxylation. since the addition of xenopus and sparus bgp sequenc ... | 1995 | 8567186 |
| role of extracellular products in the pathogenicity of vibrio strains on cultured gilt-head seabream (sparus aurata). | the enzymatic activities of extracellular products (ecp) from different pathogenic vibrio strains and their virulence for fish (cultured gilt-head seabream, sparus aurata l.) were tested. ecp were obtained by growing the bacteria on cellophane overlays. cytotoxic effects of ecp on fish cell lines were rapid and strong, provoking mortality of fish after 24 to 72 h of the ecp inoculation. a close relationship between proteolytic activities (gelatinase and caseinase) of the ecp and the presence of ... | 1995 | 8588839 |
| amino acid composition and endocrine control of vitelline envelope proteins in european sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata). | the vitelline envelopes of european sea bass and gilthead sea bream are both composed of mainly four proteins with the molecular masses of 90, 52, 48, 45 kda and 75, 50, 48, 44 kda, respectively. each protein has an amino acid composition that is characterized by a high content of proline and glutamic acid and a low content of cysteine, similar to the whole vitelline envelope of both species. the amino acid composition suggests that each protein is distinct but related to the other vitelline env ... | 1995 | 8588933 |
| nonspecific cell-mediated cytotoxicity in the seawater teleosts (sparus aurata and dicentrarchus labrax): ultrastructural study of target cell death mechanisms. | in higher vertebrates, cytolytic lymphocytes lyse their targets by the mechanisms of cell death called necrosis and apoptosis. however, the mechanisms of target cell death mediated by fish cytotoxic cells have not been established. we report the ultrastructure of target cell death mediated by the seawater teleosts gilthead seabream (sparus aurata) and sea bass (dicentrarchus labrax). | 1996 | 8694285 |
| immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish. | using antisera to regions of human parathyroid hormone-related protein (pthrp) the saccus vasculosus (sv) of the sea bream (sparus aurata) has been shown to contain immunoreactive pthrp. by immunohistochemistry (ihc) the epithelial coronet cells in fixed and wax-embedded sv tissue reacted with antisera to the prepro region of human pthrp (-13 to +2), the n-terminus pthrp (1-16), and the midmolecule pthrp (50-69). sodium dodecyl sulfate-polyacrylamide gel electrophoresis of saccus extracts and in ... | 1996 | 8713647 |
| ontogeny of growth hormone protein and mrna in the gilthead sea bream sparus aurata. | the ontogeny of growth hormone (gh) protein and mrna was studied in the gilthead sea bream sparus aurata by northern blot, reverse transcription-polymerase chain reaction (rt-pcr) and western blot analyses. total rna was prepared from a pool of larvae collected on different days after hatching. when hybridized to sparus aurata gh cdna, gh specific mrna was first seen on day 3 post-hatching. levels increased on subsequent days and reached the highest levels on day 9; thereafter a decrease was not ... | 1996 | 8717446 |
| somatotropin release-inhibiting factor and galanin innervation in the hypothalamus and pituitary of seabream (sparus aurata). | the distribution of galanin (gal) and somatotropin-release-inhibiting-factor (srif) immunoreactivity in the hypothalamus and pituitary of the sea bream (sparus aurata) was studied by immunocytochemistry. an extensive system of neurons immunoreactive with antisera to the two peptides was identified throughout the brain with staining particularly in the hypothalamus. in the hypothalamus, gal immunoreactive perikarya were detected principally in the nucleus preopticus and nucleus tuberis. major ner ... | 1996 | 8729936 |
| early organization of the pituitary gland in sparus aurata l. (teleostei). an ultrastructural study. | the cell organization of the pituitary gland and the relationship between neurohypophysis and adenohypophysis in early developmental stages of the gilthead sea bream, sparus aurata, were studied by electron microscopy. in newly hatched larvae, the pituitary gland was embedded in the ventral floor of the diencephalon and separated from the hypothalamus by a continuous basal lamina. elongated mesenchymal cells next to the ventral surface were observed. at this stage, there was no neurohypophysis a ... | 1996 | 8729962 |
| light microscopic description of eimeria sparis sp. nov. and goussia sparis sp. nov. (protozoa:apicomplexa) from sparus aurata l. (pisces: teleostei). | two new species of coccidia, eimeria sparis sp. nov. and goussia sparis sp. nov., were found in the intestine of sparus aurata from different culture systems of spain. these are the first coccidian species described from this host. e. sparis is distinguished from other eimeria spp. from perciformes in the size and shape of the sporocysts, which measure 6-9.7 (mean = 7.78, sd = 1.33) x4-6.5 (5.3 +/- 0.92) microns, and in the stieda-like body. mature oocysts, spherical or subspherical, measure 9.4 ... | 1996 | 8740548 |
| crowding stress induces changes in serum haemolytic and agglutinating activity in the gilthead sea bream sparus aurata. | sea bream sparus aurata were subjected to crowding for 3 weeks and the levels of plasma cortisol and glucose were determined in order to ascertain the occurrence of stress. at the same time, selected indicators of the immune response were monitored through blood lymphocyte counting and selected humoral responses such as the haemagglutination activity (ha) of serum towards rabbit erythrocytes (rarbc) and the alternative complement pathway (acp) levels. the results show an initial moderate increas ... | 1996 | 8797287 |
| development of enzyme immunoassays for 3,5,3'-triiodo-l-thyronine and l-thyroxine: time-course studies on the effect of food deprivation on plasma thyroid hormones in two marine teleosts, sea bass (dicentrarchus labrax l.) and sea bream (sparus aurata l.). | the effects of short-term food deprivation and photoperiod on plasma thyroid hormone levels of sea bass and sea bream were studied. animals were acclimated under constant photoperiod regime (15l/9d) and feeding times (2 hr after light onset and 2 hr before light offset). time-course studies involved monitoring plasma hormone levels every 4 hr throughout 1.5 24-hr cycles. plasma 3,5, 3'-triiodo-l-thyronine (t3) and l-thyroxine (t4) were assayed using a newly developed competitive enzyme immunoass ... | 1996 | 8812399 |
| kinetics of adhesion of selected fish-pathogenic vibrio strains of skin mucus of gilt-head sea bream (sparus aurata l.). | the kinetics of adhesion of vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. a modified langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (vibrio alginolyticus dp1he4 and vibrio anguillarum-like dc12r8 and dc12r9) showed a saturation kinetics except for v. alginolyticus (can), which showed a proportional adsorption kinetics. the adhesive capability for skin mucus does not seem to be an essential viru ... | 1996 | 8837420 |
| three forms of gonadotropin-releasing hormone in a perciform fish (sparus aurata): complementary deoxyribonucleic acid characterization and brain localization. | three forms of gnrh-salmon (sgnrh), seabream (sb-gnrh), and chicken (cgnrh-ii)-have been described in the gilthead seabream (sparus aurata) brain, and the cdna encoding the sbgnrh precursor was recently isolated. in the present study, the cdnas encoding the sgnrh and cgnrh-ii were isolated and characterized, and the neurons producing the three gnrhs were localized in the seabream brain. fragments of sgnrh and cgnrh-ii cdnas were amplified by polymerase chain reaction and used as probes to isolat ... | 1996 | 8862782 |
| molecular cloning of fish alcohol dehydrogenase cdna. | a cdna encoding a putative alcohol dehydrogenase class iii (adh) was cloned from a cdna library constructed from 7-day larvae rna of the marine teleost sparus aurata. the full length cdna is 1350 nucleotides (nt) long and contains an orf of 1128 nt [encoding 376 amino acid (aa) residues]. identity of 82% was found with human class iii adh (305 of 372 aa compared), and only 62% identity with a fish (cod) adh (234 of 375 aa compared). northern hybridization analysis with the cdna revealed a transc ... | 1996 | 8863743 |
| hematological recovery in sparus aurata after bleeding. a time course study. | to determine the hematological recovery after blood loss in gilthead sea bream juveniles, an experimental anemia was induced by caudal blood extraction. seventy fish were used for experimental hemorrhage and another 35 were used as controls. blood samples were taken after 0.5, 1, 2, 4, 8, 16 and 32 days in both control and anemic groups. after bleeding, besides a reduction in the direct hematic constants, a peak in the mean cell volume value, together with a decrease in mean cell hemoglobin conc ... | 1995 | 8907437 |
| cloning and expression of somatolactin, a pituitary hormone related to growth hormone and prolactin from gilthead seabream, sparus aurata. | a pituitary hormone, somatolactin (sl), belonging to the gh/prl family, is produced in the intermediate lobe of the teleost pituitary. the function of this protein is uncertain. clones coding for sl were isolated and sequenced from a gilthead seabream pituitary cdna expression library. the nucleotide sequence of the larger cdna isolated was 1.5 kb containing a 0.8-kb 3'-untranslated region and two potential polyadenylation signals (aataaa). the mature polypeptide is composed of 207 amino acids, ... | 1996 | 8954766 |
| growth hormone as a function of age and dietary protein: energy ratio in a marine teleost, the gilthead sea bream (sparus aurata). | we examined in a factorial design the effect of dietary protein (45%, 52% and 60%) and lipids (8%, 12%, 17%) on growth performance and circulating growth hormone (gh) levels of fingerling sea bream (5-month-old) fed to satiation with self-feeders. daily weight gain (2.6-2.9%) and feed gain ratio (1.1-1.3) of fish fed high protein-low lipid diets were comparable to those found in fast growing strains of rainbow trout. however, increasing hyperphagia in association with the decrease of daily weigh ... | 1996 | 8971555 |
| ultrastructure and cytochemistry study of goussia sparis (protozoa: apicomplexa) stages from the intestine of the gilthead sea bream sparus aurata l. (pisces: teleostei). | the ultrastructure and cytochemistry of merogonial and gamogonial stages and early unsporulated oocysts of goussia sparis in the intestine of sparus aurata were studied. the typical pellicle was observed in some stages. the different stages might appear in intraepithelial or supraepithelial positions, but they were always intracellular. first steps of two apparently different endomerogonies were observed in intra- and supraepithelial positions, respectively. an apparent ectomerogony also occurre ... | 1997 | 9000229 |
| response of eosinophilic granule cells of gilthead seabream (sparus aurata, teleostei) to bacteria and bacterial products. | eosinophilic granule cells in the gills and peritoneal exudate of gilthead seabream (sparus aurata l.) are characterized by the presence of prominent eosinophilic granules in their cytoplasm and are here described for the first time. the oval granules of these cells contain an electron-dense inclusion surrounded by a less dense filamentous matrix and are peroxidase- and acid phosphatase-negative. unlike other granulocytes of gilthead seabream, eosinophilic granule cells do not ingest bacteria in ... | 1997 | 9011398 |
| growth hormone as an in vitro phagocyte-activating factor in the gilthead sea bream (sparus aurata) | the stimulatory action of growth hormone on gilthead sea bream phagocyte-enriched cultures was demonstrated in vitro for the first time in a fish species. phagocytes consisted mainly of macrophages, with a small number of neutrophils and eosinophils. macrophages were unequivocally identified by their esterase staining and the lack of myeloperoxidase staining. cells primed with recombinant rainbow trout gh showed clear morphological (light- and scanning electron-microscopic) and functional differ ... | 1997 | 9023084 |
| molecular cloning of gilthead seabream (sparus aurata) pituitary transcription factor ghf-1/pit-1. | we report here the complete nucleotide sequence of a cdna clone encoding sparus aurata ghf-1/pit-1 isolated from an expression library prepared from gilthead seabream pituitary gland poly(a)+ rna. the cdna sequence (saghf-1/pit-1) encodes a protein of 371 amino acids (aa) containing a pou domain (aa 194-343) and a transactivation, sta domain (aa 1-128). northern blot hybridization of pituitary rna detected a single 3.0 kb band and a rat ghf-1/pit-1 antiserum was found to immunoreact with pituita ... | 1997 | 9034317 |
| presence and activity of compounds with gnrh-like activity in the ovary of seabream sparus aurata. | the binding and activity of gonadotropin-releasing hormone (gnrh) were characterized in the mature gilthead seabream (sparus aurata) ovary by use of an analogue of salmon gnrh([d-arg6,trp7,leu8,pro9-n(et)]gnrh, sgnrh-a) as labeled ligand. the binding of 125i-sgnrh-a to the seabream ovarian membrane preparation was saturable, displaceable, reversible, and dependent on time, temperature and tissue concentration. addition of unlabeled s-gnrh-a displaced the radio-ligand in a dose-related manner, in ... | 1997 | 9038998 |
| ultrastructure and cytochemistry study of eimeria sparis (protozoa:apicomplexa) stages from the intestine of gilthead sea bream sparus aurata l. (pisces:teleostei). | the ultrastructure and cytochemistry of merogonial, gamogonial, and early sporogonial stages of eimeria sparis in the intestine of sparus aurata were studied. mature stages showed the typical pellicle, which was lost in some transitional stages. an apparent unit membrane was seen in some immature stages. a parasitophorous vacuole, sometimes with membrane vesicular invaginations, was usually observed. we propose that the stages located over the epithelium and the so-called epicellular stages be t ... | 1997 | 9039694 |
| structural c3 diversity in fish: characterization of five forms of c3 in the diploid fish sparus aurata. | in virtually all species examined to date, the functionally active third component of complement (c3) is encoded by a single gene. we have recently demonstrated, however, that trout possess three structurally and functionally distinct active c3 that represent the products of at least two different c3 genes. in the present study, we provide evidence that multiple forms of functional c3 occur not only in the rainbow trout (salmo gairdneri), a quasi-tetraploid old teleost fish, but also in the dipl ... | 1997 | 9058817 |
| effect of caulerpenyne, a toxin extracted from caulerpa taxifolia on mechanisms regulating intracellular ph in sea urchin eggs and sea bream hepatocytes. | the proliferation of the green marine alga caulerpa taxifolia in the mediterranean led us to investigate the toxic effects on marine organisms of caulerpenyne (cyn), the major secondary metabolite synthesized by the alga. this study was performed on sea urchin eggs (paracentrotus lividus) and isolated hepatocytes from the sea bream (sparus aurata), in which accumulation of the toxins by metabolic processes may be of significance. cyn provoked an acidification of seawater containing both unfertil ... | 1996 | 9110246 |
| recombinant gilthead seabream (sparus aurata) insulin-like growth factor-i: subcloning, expression in escherichia coli, purification and characterization. | gilthead seabream (sparus aurata) insulin-like growth factor-i (gsigf-i) cdna coding for the mature protein was cloned in a pgem-3z vector, and then transferred into prokaryotic expression vector pet-11a and expressed in escherichia coli bl21(de3) cells upon induction with isopropyl thiogalactoside. the expressed protein contained within the inclusion-body pellet was solubilized in 4.5 m urea, refolded for 24 h at ph 11.3 in the presence of catalytic amounts of cysteine and purified to over 98% ... | 1997 | 9135579 |
| ceratomyxa sparusaurati (protozoa:myxosporea) infections in cultured gilthead sea bream sparus aurata (pisces:teleostei) from spain: aspects of the host-parasite relationship. | ceratomyxa sparusaurati infection was studied in gilthead sea bream from different mariculture systems of spain. culture conditions were found to influence the infection dynamics. total prevalences ranged from 0 in a closed system to 59.9% in a mediterranean semi-intensive farm. prevalence was significantly lower in summer in a restricted group from the latter system, but no clear seasonal pattern was observed in the remaining fish groups in any system. a statistically significant dependence bet ... | 1997 | 9211504 |
| regeneration of skeletal muscle in two teleost fish: sparus aurata and brachydanio rerio. | regeneration of skeletal muscle was studied in the sea bream sparus aurata, in which extensive post-larval muscle hyperplasia contributes to its large adult size, and in the zebrafish brachydanio rerio, which shows little post-larval hyperplasia and reaches only a small adult size. small mechanical lesions of body wall muscle were made under general anaesthesia, and the progress of subsequent regeneration was assessed at various intervals by histology and electron microscopy (for general morphol ... | 1997 | 9211834 |
| cloning and sequencing of a full-length sea bream (sparus aurata) beta-actin cdna. | a full-length cdna clone encoding beta-actin (beta-actin) was isolated from a sea bream (sparus aurata) liver cdna library. sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. the sea bream beta-actin sequence showed 98% identity to carp and human beta-actin and 95% and 94% identity to sea squirt and dictyostelium cytoplasmic actins, respectively. | 1997 | 9226879 |
| first events in lipid absorption during post-embryonic development of the anterior intestine in gilt-head sea bream | structural development and lipid absorption in anterior intestine of gilt-head sea bream sparus aurata, were studied by light and electron microscopy during three stages of post-embryonic development: (1) the endotrophic period from hatching day (day 0) to mouth opening day (day 3); (2) the endo-exotrophic period from days 3 to 15; (3) the exotrophic period after day 15. during the 2 days following hatching, there was no trace of lipids in intestinal epithelia. before mouth opening day, the firs ... | 1997 | 9236098 |
| edwardsiellosis in wild striped bass from the chesapeake bay. | the first epizootic of edwardsiellosis, caused by edwardsiella tarda, is described. the epizootic occurred in the chesapeake bay, maryland (usa) during the summer and autumn of 1994, and affected wild adult striped bass (morone saxatilis). clinical signs included numerous irregular coalescing hemorrhagic ulcers on the body and fins that were distinctly malodorous. internally, the body cavity was filled with abundant yellowish or sanguinous mucoid fluid, and the visceral organs had multiple tiny ... | 1997 | 9249698 |
| localization of igf-i and igf-i receptor mrna in sparus aurata larvae. | studies of the ontogeny of igf-i mrna during embryonic and larval development of the gilthead sea bream sparus aurata showed its expression during these early developmental stages. the present study applies in situ hybridization to localize igf-i and igf receptor mrnas in 16-day larvae of s. aurata. paraffin sections were hybridized with homologous rna probes labeled by [35s]utp. igf-i mrna expression was found mainly in chondrocytes, in both the gill arches and cranial cartilage, in skeletal mu ... | 1997 | 9268610 |
| diversity of the third form of complement, c3, in fish: functional characterization of five forms of c3 in the diploid fish sparus aurata. | we have recently shown that sparus aurata, the gilthead sea bream (a diploid species), similarly to rainbow trout (a quasi-tetraploid species), possesses multiple forms of the third form of complement (c3). in the present study we have evaluated the ability of the gilthead sea bream proteins to function as active c3 molecules. all five c3 isoforms could be fixed covalently to sheep erythrocyte ghosts and were able to bind to various complement-activating surfaces in the presence of mgegta. in th ... | 1997 | 9307040 |
| ontogeny of immunoreactive somatolactin cells in the pituitary of gilthead sea bream (sparus aurata l., teleostei). | this is the first report on the identification of somatolactin (sl) cells during the early developmental stages of the teleost fish sparus aurata. the sl cells were followed from newly hatched until 46 months. sl cells were immunocytochemically identified at light microscopical level with anti-cod sl in the developing pituitary using the peroxidase-antiperoxidase method. sl cells first appeared in newly hatched specimens, in which the pituitary gland lacked the neurohypophysis. they were scarce ... | 1997 | 9310314 |
| adrenergic stimulation of sea bream (sparus aurata) red blood cells in normoxia and anoxia: effects on metabolism and on the oxygen affinity of haemoglobin | the metabolic response of sea bream (sparus aurata) red blood cells to adrenergic stimulation was determined in normoxia and anoxia. in the presence of oxygen, red blood cells swelled and then recovered their resting volume. continuous monitoring of oxygen uptake displayed the kinetics of the increase in the oxygen affinity of haemoglobin. cell volume recovery correlated with an activation of atp consumption, and the energy equilibrium was restored by increasing the rates of respiration and glyc ... | 1997 | 9318750 |
| preovulatory changes in the levels of three gonadotropin-releasing hormone-encoding messenger ribonucleic acids (mrnas), gonadotropin beta-subunit mrnas, plasma gonadotropin, and steroids in the female gilthead seabream, sparus aurata. | gilthead seabream females undergo daily cycles of final oocyte maturation (fom), ovulation, and spawning throughout their spawning season. fom consists of lipid droplet and yolk granule coalescence, germinal vesicle (gv) migration, and gv breakdown. plasma maturational gonadotropin (gth-ii) levels fluctuate throughout the day, reaching a peak at 8 h before spawning, when the gv is at the periphery of the oocyte. the preovulatory gth-ii surge is accompanied by an increase in the plasma levels of ... | 1997 | 9369182 |
| piscine (sparus aurata) alpha subunit of the g-protein transducin is homologous to mammalian cone and rod transducin. | a novel cdna encoding alpha subunit of the gtp-binding protein, transducin, has been cloned from a marine fish, sparus aurata. the cdna contains an open reading frame of 1050 nt (encoding 350 amino acid residues). a high degree of identity was found with known mammalian transducin proteins of cones (gt2 alpha) or rods (gt1 alpha): human gt2 alpha (80.2%), bovine gt2 alpha (79.3%), mouse tt1 alpha (78.2%), mouse gt2 alpha (78%) and bovine gt1 alpha (77.9%). northern blot analysis of different tis ... | 1997 | 9373680 |
| adrenocortical and adrenomedullary homologs in eight species of adult and developing teleosts: morphology, histology, and immunohistochemistry. | morphology, histology, and immunohistochemistry of the adrenocortical and adrenomedullary homologs (adrenal glands) of the following developing and adult teleosts were examined: salmoniformes-oncorhynchus mykiss (rainbow trout), salmo trutta fario (brown trout), coregonus lavaretus (white fish); cyprinodontiformes-gambusia affinis (mosquito fish). perciformes-dicentrarchus labrax (sea bass), sparus aurata (sea bream), diplodus sargus (white bream), oblada melanura (saddled bream). the anatomical ... | 1997 | 9405124 |
| identification and spatial distribution of the mrna encoding the gp49 component of the gilthead sea bream, sparus aurata, egg envelope. | a cdna encoding the precursor of one of the major components of gilthead sea bream, sparus aurata, egg envelope has been cloned by reverse transcriptase polymerase chain reaction (rt-pcr) techniques. the clone was isolated starting from total rna extracted from the liver of spawning female fish and estradiol-17 beta-treated male fish. sequence analysis revealed that the cdna encoded a protein of 405 aa corresponding to 49-kda component (termed gp49), a glycoprotein belonging to the n-linked type ... | 1998 | 9406196 |
| histochemical study of lymphocystis disease in skin of gilthead seabream, sparus aurata l. | a battery of horseradish peroxidase-conjugated lectins (con a, wga and dba), as well as conventional histochemical techniques (pas, saponification, alcian blue ph 0.1, 1, 2.5, chlorhydric hydrolisis, sialidase, bromophenol blue, tioglycollate reduction and ferric-ferricyanide-feiii) were used to study the content and distribution of carbohydrates, proteins and glycoconjugate sugar residues on the skin and on the lymphocystis-infected cells of gilthead seabream, sparus aurata. variable amounts of ... | 1998 | 9476632 |
| floor plate and the subcommissural organ are the source of secretory compounds of related nature: comparative immunocytochemical study. | the subcommissural organ of vertebrates secretes glycoproteins into the third ventricle that condense to form reissner's fiber (rf). antibodies raised against the bovine rf-glycoproteins reacted with the floor plate (fp) cells of two teleost (oncorhynchus kisutch, sparus aurata) and two amphibian (xenopus laevis, batrachyla taeniata) species. at the ultrastructural level, the immunoreactivity was confined to secretory granules, mainly concentrated at the apical cell pole. in the rostro-caudal ax ... | 1998 | 9482230 |
| histochemistry of carbohydrates, proteins and lipids during swimbladder development in seabream, sparus aurata and seabass, dicentrarchus labrax. | some histochemical aspects of carbohydrates, proteins and lipids were studied in the swimbladder of seabream, sparus aurata and seabass, dicentrarchus labrax. in both species, swimbladder development occurs in two stages: (1) inflation and (2) expansion, respectively. no important differences in histochemical distribution of glucidic, proteic and lipidic macromolecules were observed during the swimbladder development. glycoproteins containing mannose and/or glucose sugar are present in the swimb ... | 1997 | 9491314 |
| the post-larval development of lateral musculature in gilthead sea bream sparus aurata (l.) and sea bass dicentrarchus labrax (l.). | fibre-type differentiation of lateral musculature has been studied in gilthead sea bream sparus aurata (l.) and sea bass dicentrarchus labrax (l.) during post-larval development using ultrastructural, histochemical and morphometric techniques. the study showed three muscle layers: red, intermediate (or pink) and white. initially, most of the red muscle showed low myosin atpase (m-atpase) activity fibres, whereas near the transverse septum some small high m-atpase activity fibres appeared and lat ... | 1998 | 9505442 |
| insulin during the early postembryonic development of the gilt-head sea bream, sparus aurata: ultrastructural, immunohistochemical, and biochemical studies. | insulin was sought during the early postembryonic development of gilt-head sea bream, sparus aurata, using ultrastructural, immunohistochemical and biochemical techniques. the endocrine pancreas appeared at hatching (day 0) as a single cluster of morphologically similar cells. secretory granules formed from day 1 onwards but the cells could only be identified as insulin-producing b cells at the end of the endotrophic period (day 3-day 4). insulin-immunoreactive cells were detected in the pancrea ... | 1998 | 9570935 |
| chemotaxis of pathogenic vibrio strains towards mucus surfaces of gilt-head sea bream (sparus aurata l.). | vibrio anguillarum and vibrio alginolyticus exhibited significant adhesion to and chemotactic abilities towards mucus collected from the skin, gills, and intestine of gilt-head sea bream. quadratic polynomial models for chemotaxis designed to estimate the influence of temperature demonstrated a differential bacterial chemotaxis depending of the source of the mucus, with the chemotaxis towards intestinal mucus being the least influenced. | 1998 | 9575135 |
| distribution of vasoactive intestinal peptide in the brain and hypothalamo-hypophysial system of the sea bream (sparus aurata). | 1998 | 9629174 | |
| methodological aspects of assessing phagocytosis of vibrio anguillarum by leucocytes of gilthead seabream (sparus aurata l.) by flow cytometry and electron microscopy. | in this paper we optimize a flow cytometric method for evaluating the phagocytic activity of leucocytes in gilthead seabream (sparus aurata l.) and characterize the phagocytic cells observed. optimal conditions were established for the fluorescein-labelling and analysis of the bacterium vibrio anguillarum by flow cytometry. head-kidney leucocytes were incubated with the heat-killed fluorescein isothiocyanate (fitc)-labelled bacteria for different periods, during which the kinetics of phagocytosi ... | 1998 | 9634605 |
| acetyl salmon endorphin-like and interrenal stress response in male gilthead sea bream, sparus aurata. | the present study investigates the role of melanotrope proopiomelanocortin-derived peptide in the interrenal stress response to different stressors in male gilthead sea bream, sparus aurata. plasma cortisol and acetyl salmon endorphin (acetyl s-ep), as well as pituitary acetyl s-ep contents, were measured during two stress paradigms: (a) long-term (1-month) confinement and crowding, and (b) short-term (60-min) confinement, crowding, and manipulation. in addition, naltrexone, a highly specific op ... | 1998 | 9705580 |
| muscle development in gilthead sea bream (sparus aurata, l.) and sea bass (dicentrarchus labrax, l.): further histochemical and ultrastructural aspects. | the histochemical profiles--matpase and nadh-tr reactions--of the red and white muscle fibres of gilthead sea bream and sea bass were determined from the first week after hatching. modifications of the matpase technique by combinations of ph/time/molarity were carried out in order to compare the sensitivity of the myosin atpase of each muscle fibre type of the lateral muscle. results showed that the staining of muscle fibres was independent of small modifications in the technique. the intermedia ... | 1998 | 9741144 |
| pathogenicity of vibrio alginolyticus for cultured gilt-head sea bream (sparus aurata l.). | the in vivo and in vitro pathogenic activities of whole cells and extracellular products of vibrio alginolyticus for cultured gilt-head sea bream were evaluated. the 50% lethal doses ranged from 5.4 x 10(4) to 1.0 x 10(6) cfu/g of body weight. the strains examined had the ability to adhere to skin, gill, and intestinal mucus of sea bream and to cultured cells of a chinook salmon embryo cell line. in addition, the in vitro ability of v. alginolyticus to adhere to mucus and skin cells of sea bream ... | 1998 | 9797276 |
| cloning, characterisation and expression of the apolipoprotein a-i gene in the sea bream (sparus aurata). | a full length cdna clone representing apolipoprotein a-i was isolated from a sea bream (sparus aurata) liver library. the clone encodes a 261 amino acid protein which shows highest amino acid identity (38%) with salmon apolipoprotein a-i. northern blot analysis showed strong expression of a 1.4 kb transcript in liver with lower expression in intestine. expression of apolipoprotein a-i in intestine was markedly reduced by treatment with triiodothyronine (t3). | 1998 | 9804999 |
| different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle. | the presence of putative neuromodulators in the nerve fibres was investigated in white skeletal muscle of two teleost fish not taxonomically correlated and showing different patterns of innervation (multiple versus focal innervation). cryostat sections of epaxial, hypaxial and adductor mandibulae (am) muscles of sparus aurata and anguilla anguilla were stained histochemically for reduced nicotinamide adenine dinucleotide phosphate (nadph)-diaphorase. other sections were used for indirect immunoh ... | 1998 | 9810486 |