Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| insertional mutagenesis in drosophila. ii. p element mediated transformation of drosophila yakuba. | drosophila yakuba, a member of melanogaster subgroup being free of p element, acquired resistance to an antibiotic neomycin by the transformation utilizing p element. in this species, the transformation frequency was comparable to that of d. melanogaster. further, the occurrence of 8 base pairs duplication upon the insertion of the element was confirmed. these facts suggest that the p element could be inserted into the genome in the same manner, even in d. yakuba. any consensus for preferential ... | 1992 | 1338284 |
| population structure and mitochondrial dna gene flow in old world populations of drosophila subobscura. | an extensive survey of mitochondrial dna (mtdna) restriction polymorphism in 156 isofemale lines from 29 different geographic populations of drosophila subobscura distributed throughout the old world was carried out. ten restriction enzymes were used, five of which revealed restriction site polymorphism. of the 31 restriction sites detected, 13 were found to be polymorphic. comparisons with the mtdna map of drosophila yakuba indicate that the variable sites are mainly concentrated in protein gen ... | 1992 | 1346531 |
| mitochondrial transfer rna genes in a black fly, simulium vittatum (diptera: simuliidae), indicate long divergence from mosquito (diptera: culicidae) and fruit fly (diptera: drosophilidae). | sequences are given for nine complete genes and one partial mitochondrial trna gene of the black fly, simulium vittatum (zetterstedt). sequenced trna genes were for alanine, arginine, asparagine, aspartic acid, glutamic acid, glycine, leucine(cun), lysine, serine(agn), and valine. nucleotides were aligned with the same previously sequenced genes in aedes albopictus skuse and drosophila yakuba burla. a cluster of six trna genes, which differ in arrangement in ae. albopictus and d. yakuba, was amp ... | 1992 | 1379642 |
| population genetics of polymorphism and divergence. | frequencies of mutant sites are modeled as a poisson random field in two species that share a sufficiently recent common ancestor. the selective effect of the new alleles can be favorable, neutral, or detrimental. the model is applied to the sample configurations of nucleotides in the alcohol dehydrogenase gene (adh) in drosophila simulans and drosophila yakuba. assuming a synonymous mutation rate of 1.5 x 10(-8) per site per year and 10 generations per year, we obtain estimates for the effectiv ... | 1992 | 1459433 |
| a local algorithm for dna sequence alignment with inversions. | a dynamic programming algorithm to find all optimal alignments of dna subsequences is described. the alignments use not only substitutions, insertions and deletions of nucleotides but also inversions (reversed complements) of substrings of the sequences. the inversion alignments themselves contain substitutions, insertions and deletions of nucleotides. we study the problem of alignment with non-intersecting inversions. to provide a computationally efficient algorithm we restrict candidate invers ... | 1992 | 1591531 |
| putative origin and function of the intergenic region between coi and coii of apis mellifera l. mitochondrial dna. | the mitochondrial genome of honeybees is characterized by the presence of a long intergenic sequence located between the coi and coii genes. in addition, the length of this sequence varies between and within subspecies. four length categories (200, 250, 450 and 650 bp) have been found in 63 sampled colonies. analysis of the sequence of the largest type reveals the existence of two units: p (54 bp, 100% a + t) and q (196 bp, 93.4% a + t). the lengths encountered in the sample are explained by the ... | 1991 | 1649072 |
| mitochondrial dna of the blowfly phormia regina: restriction analysis and gene localization. | a study of an invertebrate mitochondrial genome, that of the blowfly phormia regina, has been initiated to compare its structural and functional relatedness to other metazoan mitochondrial genomes. a restriction map of mitochondrial dna (mtdna) isolated from sucrose gradient-purified mitochondria has been established using a combination of single and double restriction endonuclease digestions and hybridizations with isolated mtdna fragments, revealing a genome size of 17.5 kilobases (kb). a numb ... | 1991 | 1652934 |
| identification of nucleotide substitutions necessary for trans-activation of mariner transposable elements in drosophila: analysis of naturally occurring elements. | six copies of the mariner element from the genomes of drosophila mauritiana and drosophila simulans were chosen at random for dna sequencing and functional analysis and compared with the highly active element mos1 and the inactive element peach. all elements were 1286 base pairs in length, but among them there were 18 nucleotide differences. as assayed in drosophila melanogaster, three of the elements were apparently nonfunctional, two were marginally functional, and one had moderate activity th ... | 1991 | 1655567 |
| evidence for interspecific transfer of the transposable element mariner between drosophila and zaprionus. | the transposable element mariner occurs widely in the melanogaster species group of drosophila. however, in drosophilids outside of the melanogaster species group, sequences showing strong dna hybridization with mariner are found only in the genus zaprionus. the mariner sequence obtained from zaprionus tuberculatus is 97% identical with that from drosophila mauritiana, a member of the melanogaster species subgroup, whereas a mariner sequence isolated from drosophila tsacasi is only 92% identical ... | 1991 | 1664000 |
| molecular evolution in the drosophila yakuba period locus. | two nuclear genes from drosophila yakuba were cloned, the orthologue of the drosophila melanogaster period (per) clock gene and the orthologue of an unnamed d. melanogaster gene adjacent to per, which encodes a 0.9-kb rna transcript. the dna and presumed protein sequences of both genes are presented and compared with their orthologues in d. melanogaster. consistent with the per orthologues described in other drosophila species, some parts of the per gene have accumulated nonsynonymous substituti ... | 1990 | 1702156 |
| codon usage is imposed by the gene location in the transcription unit. | a characteristic profile of the fluctuations of codon usage is observed in bacteriophages and mitochondria. by following the dna in the direction of transcription, one moves slowly from a region where selective pressure favours codons ending with c to a region where the bias is in favour of codons ending with t; then, abruptly, one again enters a region of codons ending in c. the transcription end point takes place in the area of abrupt change in codon usage. by comparing drosophila yakuba and m ... | 1991 | 1807825 |
| comparison of mitochondrial dna sequences of seven morphospecies of black flies (diptera: simuliidae). | universal primers constructed from the 16s ribosomal rna gene in the drosophila yakuba mitochondrial genome were successfully used to amplify, via the polymerase chain reaction, the homologous region of mitochondrial dna from seven black fly morphospecies. amplification was achieved from single larval salivary glands and from single adults preserved in carnoy's fixative (ethanol - acetic acid, 3:1), allowing dna sequences and polytene chromosome banding pattern data to be gathered from the same ... | 1991 | 2055453 |
| discrepancy in divergence of the mitochondrial and nuclear genomes of drosophila teissieri and drosophila yakuba. | restriction sites were compared in the mitochondrial dna (mtdna) molecules from representatives of two closely related species of fruit flies: nine strains of drosophila teissieri and eight strains of drosophila yakuba. nucleotide diversities among d. teissieri strains and among d. yakuba strains were 0.07% and 0.03%, respectively, and the nucleotide distance between the species was 0.22%. also determined was the nucleotide sequence of a 2305-nucleotide pair (ntp) segment of the mtdna molecule o ... | 1990 | 2115928 |
| cloning of the mitochondrial genome of anopheles quadrimaculatus. | the entire 15 kilobase (kb) anopheles quadrimaculatus mitochondrial dna (mtdna) was cloned as three ecori fragments in a bacteriophage vector and then subcloned into plasmid vectors. the cloned dna was physically mapped with restriction endonucleases, and the maps were compared to the restriction patterns of native a. quadrimaculatus mtdna. several genes were mapped by sequencing the ends of a. quadrimaculatus mtdna subclones and by hybridization with the previously characterized aedes albopictu ... | 1982 | 2134168 |
| mitochondrial dna of chlamydomonas reinhardtii: the gene for apocytochrome b and the complete functional map of the 15.8 kb dna. | we have sequenced the termini of the mitochondrial genome of chlamydomonas reinhardtii and now present the dna sequence of the gene for apocytochrome b. this gene is the thirteenth gene of the linear 15.8 kb dna and appears to be the last one of the mt genome. the deduced protein sequence of 381 amino acid residues shows 56%, 48.6% and 48% identity with the apocytochrome b proteins of maize, drosophila yakuba and mouse, respectively. rna analysis reveals a transcript of about 1250 nucleotides. i ... | 1990 | 2250648 |
| recombination and replication of plasmid-like derivatives of a short section of the mitochondrial chromosome of neurospora crassa. | the 21-kbp mitochondrial chromosome of the stp-ruv strain of neurospora crassa undergoes regional amplification yielding plasmid-like supercoiled circles varying in size from subunit length to very high multimers. a comparison of the base sequence of the five plasmids studied, with the region of the chromosome from which they were derived, indicated that the amplified chromosomal segments were determined by a recombination-excision process near or within two structurally distinctive regions. one ... | 1989 | 2524421 |
| mitochondrial dna of chlamydomonas reinhardtii: the nd4 gene encoding a subunit of nadh dehydrogenase. | the nd4 gene encoding a subunit of respiratory nadh dehydrogenase has been identified on the linear 15.8 kb mitochondrial dna of chlamydomonas reinhardtii. the gene maps downstream of nd5. the 1,332 bp nucleotide sequence presented is the first complete reported nd4 sequence from a photoautotrophic organism. the deduced protein of 443 amino acid residues shows 34%, 29% and 27% homology to the protein sequences of aspergillus amstelodami, drosophila yakuba and mouse, respectively. nd4 is the fift ... | 1989 | 2791036 |
| different mitochondrial gene orders among insects: exchanged trna gene positions in the coii/coiii region between an orthopteran and a dipteran species. | we have cloned and sequenced a 2.65 kb segment of the mtdna molecule of the orthopteran insect locusta migratoria. it harbors the genes for four mitochondrial trnas, for cytochrome c oxidase subunits ii and iii and for atpase subunits 6 and 8. the order of the locust genes resembles that of drosophila yakuba: in both insects the genes for coii and atpase 8 are separated from each other by the genes encoding trna(lys) and trna(asp), but in the locust, the positions of the two trna genes are rever ... | 1988 | 2852067 |
| the ribosomal rna genes of drosophila mitochondrial dna. | the nucleotide sequence of a segment of the mtdna molecule of drosophila yakuba which contains the a+t-rich region and the small and large rrna genes separated by the trnaval gene has been determined. the 5' end of the small rrna gene was located by s1 protection analysis. in contrast to mammalian mtdna, a trna gene was not found at the 5' end of the d. yakuba small rrna gene. the small and large rrna genes are 20.7% and 16.7% g+c and contain only 789 and 1326 nucleotides. the 5' regions of the ... | 1985 | 2989784 |
| the mitochondrial dna molecular of drosophila yakuba: nucleotide sequence, gene organization, and genetic code. | the sequence of the 16,019 nucleotide-pair mitochondrial dna (mtdna) molecule of drosophila yakuba is presented. this molecule contains the genes for two rrnas, 22 trnas, six identified proteins [cytochrome b, cytochrome c oxidase subunits i, ii, and iii (coi-iii), and atpase subunits 6 and 8] and seven presumptive proteins (urf1-6 and urf4l). replication originates within a region of 1077 nucleotides that is 92.8% a + t and lacks any open reading frame larger than 123 nucleotides. an equivalent ... | 1985 | 3001325 |
| rates of nucleotide substitution in drosophila mitochondrial dna and nuclear dna are similar. | while the majority of dna in eukaryotes is in the nucleus, a small but functionally significant amount is found in organelles such as chloroplasts and mitochondria. a recent, rather remarkable, finding has been that in vertebrates the dna in the mitochondria (mtdna) is evolving 5-10 times faster than the dna in the nucleus. no similar studies have been done with invertebrates. using the technique of dna x dna hybridization, we have measured the degree of nucleotide substitution between drosophil ... | 1986 | 3097641 |
| drosophila melanogaster mitochondrial dna: gene organization and evolutionary considerations. | the sequence of a 8351-nucleotide mitochondrial dna (mtdna) fragment has been obtained extending the knowledge of the drosophila melanogaster mitochondrial genome to 90% of its coding region. the sequence encodes seven polypeptides, 12 trnas and the 3' end of the 16s rrna and co iii genes. the gene organization is strictly conserved with respect to the drosophila yakuba mitochondrial genome, and different from that found in mammals and xenopus. the high a + t content of d. melanogaster mitochond ... | 2011 | 3130291 |
| the watermelon mitochondrial urf-1 gene: evidence for a complex structure. | we have cloned and sequenced a fragment of watermelon mitochondrial dna (mtdna) which contains a gene homologous to mitochondrial urf-1 (unidentified reading frame-1) of vertebrates, drosophila yakuba and aspergillus nidulans. urf-1 is thought to encode a component of the respiratory chain nadh dehydrogenase. two coding regions in the watermelon gene are separated by approximately 1,450 bp of untranslatable dna. these two exons encode the central portions of urf-1, and are highly conserved. we p ... | 1986 | 3447741 |
| the gene for the large (16s) ribosomal rna from the locusta migratoria mitochondrial genome. | the nucleotide sequence of a segment of the mtdna molecule of the locust locusta migratoria containing the complete large rrna (16s) gene and some nucleotides in its vicinity has been determined. the gene contains 1314 nucleotides, comprising the smallest metazoan gene reported to date. the gene has a low content of gc (21%) and exhibits an extended sequence homology to the corresponding gene of the dipteran insect drosophila yakuba, suggesting a comparable secondary structure. the gene structur ... | 1987 | 3450412 |
| sequence evolution of drosophila mitochondrial dna. | we have compared nucleotide sequences of corresponding segments of the mitochondrial dna (mtdna) molecules of drosophila yakuba and drosophila melanogaster, which contain the genes for six proteins and seven trnas. the overall frequency of substitution between the nucleotide sequences of these protein genes is 7.2%. as was found for mtdnas from closely related mammals, most substitutions (86%) in drosophila mitochondrial protein genes do not result in an amino acid replacement. however, the freq ... | 1985 | 3921427 |
| drosophila mitochondrial dna: a novel gene order. | part of the replication origin-containing a+t-rich region of the drosophila yakuba mtdna molecule and segments on either side of this region have been sequenced, and the genes within them identified. the data confirm that the small and large rrna genes lie in tandem adjacent to that side of the a+t-rich region which is replicated first, and establish that a trnaval gene lies between the two rrna genes and that urf1 follows the large rrna gene. the data further establish that the genes for trnail ... | 1982 | 6294611 |
| transfer rna genes in drosophila mitochondrial dna: related 5' flanking sequences and comparisons to mammalian mitochondrial trna genes. | genes for trnagly and trnaserucn have been identified within sequences of mtdna of drosophila yakuba. the trnagly gene lies between the genes for cytochrome c oxidase subunit iii and urf3, and all three of these genes are contained in the same strand of the mtdna molecule. the trnaserucn gene is adjacent to the urf1 gene. these genes are contained in opposite strands of the mtdna molecule and their 3' ends overlap. the structures of the trnagly and trnaserucn genes, and of the four trna genes of ... | 1983 | 6304652 |
| nucleotide sequence of a segment of drosophila mitochondrial dna that contains the genes for cytochrome c oxidase subunits ii and iii and atpase subunit 6. | the nucleotide sequence of a segment of the mtdna molecule of drosophila yakuba has been determined, within which have been identified the genes for trnaleuuur, cytochrome c oxidase subunit ii (coii), trnalys, trnaasp, urfa6l, atpase subunit 6 (atpase6), cytochrome c oxidase subunit iii (coiii) and trnagly. the genes are arranged in the order given and all are transcribed from the same strand of the molecule in a direction opposite to that in which replication proceeds around the molecule. the t ... | 1983 | 6306579 |
| genes for cytochrome c oxidase subunit i, urf2, and three trnas in drosophila mitochondrial dna. | genes for urf2, trnatrp, trnacys, trnatyr and cytochrome c oxidase subunit i (coi) have been identified within a sequenced segment of the drosophila yakuba mtdna molecule. the five genes are arranged in the order given. transcription of the trnacys and trnatyr genes is in the same direction as replication, while transcription of the urf2, trnatrp and coi genes is in the opposite direction. a similar arrangement of these genes is found in mammalian mtdna except that in the latter, the trnaala and ... | 1983 | 6314262 |
| a cluster of six trna genes in drosophila mitochondrial dna that includes a gene for an unusual trnaseragy. | genes for urf3, trnaala, trnaarg, trnaasn, trnaseragy, trnaglu, trnaphe, and the carboxyl terminal segment of the urf5 gene have been identified within a sequenced segment of the mtdna molecule of drosophila yakuba. the genes occur in the order given. the urf5 and trnaphe genes are transcribed in the same direction as replication while the urf3 and remaining five trna genes are transcribed in the opposite direction. considerable differences exist in the relative arrangement of these genes in d. ... | 1984 | 6324117 |
| sequence and arrangement of the genes for cytochrome b, urf1, urf4l, urf4, urf5, urf6 and five trnas in drosophila mitochondrial dna. | the nucleotide sequence of a segment of the mtdna molecule of drosophila yakuba has been determined, within which have been identified the 3' end of the large rrna gene and the entire genes for trnaleucun , urf1 , trnaserucn , cytochrome b, urf6 , trnapro, trnathr , urf4l , urf4 , trnahis and urf5 . the genes are arranged in the order given, with the large rrna gene being closest to the a+t-rich region which contains the origin of replication. transcription of all of these genes except those for ... | 1984 | 6328435 |
| the drosophila mitochondrial genome. | the mitochondrial genome of drosophila yakuba is a circular dna molecule of 16019 nucleotide pairs. the sequence contains the genes for two rrna molecules, 22 trna molecules, five known polypeptides (cytochrome b, cytochrome c oxidase subunits i, ii, iii and atpase subunit 6) and eight unidentified polypeptides (urf1, 2, 3, 4l, 4, 5, 6 and a6l). between the trna(ile) and small rrna genes there occurs a sequence of 1077 nucleotides that is 92.8 per cent a + t and lacks reading frames greater than ... | 1984 | 6400770 |
| comparative study of structure and function of blood cells from two drosophila species. | hemocytes of drosophila melanogaster and drosophila yakuba larvae have been defined in terms of their ultrastructure and functions in "coagulation", wound healing, encapsulation, phenol-oxidase activity, and phagocytosis. the position of these cells among the classical hemocyte types of insects is determined. we distinguish two plasmatocyte types (macrophage-plasmatocytes and lamellocytes) which do not seem to belong to the same lineage, and oenocytoids which are the crystal cells of the literat ... | 1982 | 6799204 |
| phylogeny of sibling species of simulium venustum and s. verecundum (diptera: simuliidae) based on sequences of the mitochondrial 16s rrna gene. | the phylogeny of four sibling species of simulium venustum (cc, cc3, cc4, and ac(gb)) and two sibling species of s. verecundum (aa and acd = s. rostratum) was reconstructed using nucleotide sequences of the mitochondrial large subunit rrna gene. separate phylogenetic analyses were performed by dividing the sequence data into: (1) helices of a computer-generated secondary structure of the rdna; (2) loops of the computer-generated structure; (3) helices of a consensus secondary structure (deduced ... | 1993 | 7519520 |
| mesoderm-specific b104 expression in the drosophila embryo is mediated by internal cis-acting elements of the transposon. | the drosophila melanogaster genome contains about 100 copies of the b104 transposable element, which is strongly expressed during embryogenesis. here we show that b104 expression is restricted to the esophageal and amnioproctodeal regions of the embryo and to the developing mesoderm. mesoderm-specific b104 expression requires the activity of the mesoderm-determining factors twist and snail. virtually the same expression patterns were observed in drosophila yakuba, a species that a separated from ... | 1995 | 7664613 |
| complete base sequence for the mitochondrial large subunit ribosomal rna of the gypsy moth lymantria dispar (l.). | a 1355 bp sequence (accession number l32141) isolated from a gypsy moth (lymantria dispar) cdna library showed 68-74% sequence identity to mitochondrial large subunit ribosomal rna (mt irrna) sequences of locusta migratoria, apis mellifera, aedes albopictus, anopheles gambiae and two drosophila species. a comparison of the primary sequences of the mt irrnas from the above insects in four orders and from esherichia coli demonstrated regions of conservation which presumably correspond to regions o ... | 1994 | 7704305 |
| analysis of mitochondrial dna and development of pcr-based diagnostic molecular markers for mediterranean fruit fly (ceratitis capitata) populations. | a 2.99 kb mtdna fragment containing two variable restriction endonuclease sites (ecorv and xbai) was subcloned and sequenced from the mediterranean fruit fly (ceratitis capitata). this fragment represents approximately one-fifth of the entire mitochondrial sequence. the sequence was aligned with the comparable region from drosophila yakuba and anopheles gambiae, resulting in 81.8% and 76.7% identity at the nucleotide level, and 77% and 67.7% identity, respectively, at the amino acid level. the s ... | 1995 | 7742977 |
| unraveling selection in the mitochondrial genome of drosophila. | we examine mitochondrial dna variation at the cytochrome b locus within and between three species of drosophila to determine whether patterns of variation conform to the predictions of neutral molecular evolution. the entire 1137-bp cytochrome b locus was sequenced in 16 lines of drosophila melanogaster, 18 lines of drosophila simulans and 13 lines of drosophila yakuba. patterns of variation depart from neutrality by several test criteria. analysis of the evolutionary clock hypothesis shows uneq ... | 1994 | 7851772 |
| partial mitochondrial dna sequence of the crustacean daphnia pulex. | a 3667-base pair (bp) fragment of the mitochondrial genome of the crustacean daphnia pulex has been sequenced and found to contain the complete genes for the small subunit ribosomal rna, nd2, seven trnas and the control region. this organization is identical to that found in drosophila yakuba mtdna yet d. pulex mtdna exhibits several unique features when compared to other mitochondrial sequences. the sequenced fragment is only 62.6% a + t which is much lower than that of any other arthropod mtdn ... | 1994 | 8082168 |
| the mitochondrial genome of anopheles quadrimaculatus species a: complete nucleotide sequence and gene organization. | the complete sequence (15,455 bp) of the mitochondrial dna of the mosquito anopheles quadrimaculatus species a is reported. this genome is compact and very a+t rich (77.4% a+t). it contains genes for 2 ribosomal rnas (rrnas), 22 transfer rnas (trnas), and 13 subunits of the mitochondrial inner membrane respiratory complexes. the gene arrangement is the same as in drosophila yakuba, except that the positions of two contiguous trnas are reversed and a third trna is transcribed from the complementa ... | 1993 | 8112570 |
| mediterranean fruit fly, ceratitis capitata (wiedemann), mitochondrial dna: genes and secondary structures for six t-rnas. | the polymerase chain reaction was used to amplify six mitochondrial t-rnas for ala, arg, asn, ser, glu and phe between genes for mitochondrial nadh dehydrogenases 3 and 5. with respect to drosophila yakuba the gene order and direction of transcription is completely conserved. analysis of secondary structure shows complete conservation of the anticodon loops but a number of differences in the dihydrouridine and t psi c loops with respect to drosophila. however, differences are such that tertiary ... | 1993 | 8269094 |
| the drosophila melanogaster dodecasatellite sequence is closely linked to the centromere and can form connections between sister chromatids during mitosis. | we have used fluorescence in situ hybridisation to wild-type and rearranged mitotic chromosomes to map the drosophila melanogaster dodecasatellite sequence. it is located at a unique site, within the pericentric heterochromatin of the right arm of the third chromosome, closely linked to the primary constriction. in polytene chromosomes, dodecasatellite is found as one or a few dots in the central region of the chromocentre. in untreated diploid cells, dodecasatellite sequences are found as one o ... | 1993 | 8360277 |
| the mitochondrial genome of the honeybee apis mellifera: complete sequence and genome organization. | the complete sequence of honeybee (apis mellifera) mitochondrial dna is reported being 16,343 bp long in the strain sequenced. relative to their positions in the drosophila map, 11 of the trna genes are in altered positions, but the other genes and regions are in the same relative positions. comparisons of the predicted protein sequences indicate that the honeybee mitochondrial genetic code is the same as that for drosophila; but the anticodons of two trnas differ between these two insects. the ... | 1993 | 8417993 |
| genome organization and restriction site map of the mitochondrial dna of the housefly (musca domestica). | a restriction map of the housefly mitochondrial dna was constructed. the putative arrangement of the major coding regions was determined by two methods. in the first, drosophila clones of known gene content were hybridized to housefly restriction fragments on southern blots. in the second, rare restriction sites and clusters of restriction sites were used to align the housefly restriction map with that of drosophila yakuba. both methods produced the same apparent gene arrangement. | 1993 | 8514159 |
| the sequence, organization, and evolution of the locusta migratoria mitochondrial genome. | the sequencing of the cloned locusta migratoria mitochondrial genome has been completed. the sequence is 15,722 bp in length and contains 75.3% a+t, the lowest value in any of the five insect mitochondrial sequences so far determined. the protein coding genes have a similar a+t content (74.1%) but are distinguished by a high cytosine content at the third codon position. the gene content and organization are the same as in drosophila yakuba except for a rearrangement of the two trna genes trnalys ... | 1995 | 8587138 |
| insect immunity: early events in the encapsulation process of parasitoid (leptopilina boulardi) eggs in resistant and susceptible strains of drosophila. | eggs of an immune suppressive strain (= virulent) of the parasitoid leptopilina boulardi are encapsulated neither in resistant nor in susceptible strains of drosophila melanogaster but are encapsulated in drosophila yakuba. eggs of a nonimmune suppressive strain (= avirulent) of the same parasitoid are encapsulated in a resistant strain of d. melanogaster and in d. yakuba but are not encapsulated in a susceptible strain of d. melanogaster. egg chorion in the 2 parasitoid strains showed the same ... | 1996 | 8587797 |
| naturally occurring variation in copia expression is due to both element (cis) and host (trans) regulatory variation. | significant differences in levels of copia [drosophila long terminal repeat (ltr) retrotransposon] expression exist among six species representing the drosophila melanogaster species complex (d. melanogaster, drosophila mauritiana, drosophila simulans, drosophila sechellia, drosophila yakuba, and drosophila erecta) and a more distantly related species (drosophila willistoni). these differences in expression are correlated with major size variation mapping to putative regulatory regions of the co ... | 1996 | 8692951 |
| partial sequence of the shrimp penaeus notialis mitochondrial genome. | about half of the mitochondrial dna of the shrimp penaeus notialis (crustacea: decapoda) has been cloned (in 2 overlapping fragments of 7.9 kb and 1 kb) and partially sequenced. the gene content and arrangement are identical to that of the homologous domain in drosophila yakuba. intergenic nucleotides are scarce and a 982 bp non-coding sequence exhibit features similar to that of mtdna control regions. the gene organization and the trna structures differentiate the penaeus notialis mitochondrial ... | 1996 | 8881281 |
| partial sequence of the mitochondrial genome of the crustacean daphnia pulex. | a 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean daphnia pulex was sequenced and found to contain the complete genes for eight trnas and five proteins (atp6, atp8, coii, coiii, nd3) and the partial sequence of coi. in combination with data described previously, approximately 50% of the d. pulex mitochondrial genome has been sequenced. the gene order in this half of the genome is identical to that of drosophila yakuba which differs from that of the other completely se ... | 1997 | 9000380 |
| mitochondrial gene arrangement of the horseshoe crab limulus polyphemus l.: conservation of major features among arthropod classes. | numerous complete mitochondrial dna sequences have been determined for species within two arthropod groups, insects and crustaceans, but there are none for a third, the chelicerates. most mitochondrial gene arrangements reported for crustaceans and insect species are identical or nearly identical to that of drosophila yakuba. sequences across 36 of the gene boundaries in the mitochondrial dna (mtdna) of a representative chelicerate. limulus polyphemus l., also reveal an arrangement like that of ... | 1997 | 9254925 |
| a screen for fast evolving genes from drosophila. | in an attempt to quantify the rates of protein sequence divergence in drosophila, we have devised a screen to differentiate between slow and fast evolving genes. we find that over one-third of randomly drawn cdnas from a drosophila melanogaster library do not cross-hybridize with drosophila virilis dna, indicating that they evolve with a very high rate. to determine the evolutionary characteristics of such protein sequences, we sequenced their homologs from a more closely related species (drosop ... | 1997 | 9275195 |
| reproductive isolation and morphogenetic evolution in drosophila analyzed by breakage of ethological barriers. | this article reports the breaking of ethological barriers through the constitution of soma-germ line chimeras between species of the melanogaster subgroup of drosophila, which are ethologically isolated. female drosophila yakuba and d. teissieri germ cells in a d. melanogaster ovary produced functional oocytes that, when fertilized by d. melanogaster sperm, gave rise to sterile yakuba-melanogaster and teissieri-melanogaster male and female hybrids. however, the erecta-melanogaster and orena-mela ... | 1997 | 9286683 |
| unusual features of the drosophila melanogaster telomere transposable element het-a are conserved in drosophila yakuba telomere elements. | het-a was the first transposable element shown to have a bona fide role in chromosome structure, maintenance of telomeres in drosophila melanogaster. het-a has hallmarks of non-long-terminal-repeat (non-ltr) retrotransposable elements but also has several unique features. we have now isolated het-a elements from drosophila yakuba, showing that the retrotransposon mechanism of telomere maintenance predates the separation of d. melanogaster and d. yakuba (5-15 million years ago). het-a elements fr ... | 0 | 9520442 |
| rate variation of dna sequence evolution in the drosophila lineages. | rate constancy of dna sequence evolution was examined for three species of drosophila, using two samples: the published sequences of eight genes from regions of the normal recombination rates and new data of the four as-c (ac, sc, l'sc and ase) and ci genes. the as-c and ci genes were chosen because these genes are located in the regions of very reduced recombination in drosophila melanogaster and their locations remain unchanged throughout the entire lineages involved, yielding less effect of a ... | 1998 | 9611206 |
| nucleotide variation in the triosephosphate isomerase (tpi) locus of drosophila melanogaster and drosophila simulans. | dna sequence variation in a 1.1-kb region including the coding portion of the tpi locus was examined in 25 homozygous third-chromosome lines of drosophila melanogaster, nine lines of drosophila simulans, and one line of drosophila yakuba. our data show that the widespread allozyme polymorphism observed in cosmopolitan d. melanogaster is due to a glutamic acid substitution occurring in a phylogenetically conserved lysine that has been identified as part of the "hinged-lid" active site of the enzy ... | 1998 | 9615457 |
| the two drosophila telomeric transposable elements have very different patterns of transcription. | the transposable elements het-a and tart constitute the telomeres of drosophila chromosomes. both are non-long terminal repeat (ltr) retrotransposons, sharing the remarkable property of transposing only to chromosome ends. in addition, strong sequence similarity of their gag proteins indicates that these coding regions share a common ancestor. these findings led to the assumption that het-a and tart are closely related. however, we now find that these elements produce quite different sets of tra ... | 1999 | 9858610 |
| courtship song rhythms in drosophila yakuba. | we studied the courtship songs of males from several strains of drosophila yakuba. there was clear evidence for two types of pulse songs, generated by the male's wing vibration, which we called 'thud' song and 'clack' song. clack song was generated by both wings, at distances further from the female than the thud component, which was produced by one-wing displays. the carrier frequency of clack song was considerably higher than that of thud, whereas the interpulse intervals (ipi) of thud song, w ... | 1999 | 10049478 |
| the drosophila gene 2a5 complements the defect in mitochondrial f1-atpase assembly in yeast lacking the molecular chaperone atp11p. | assembly of mitochondrial f1-atpase in saccharomyces cerevisiae requires the molecular chaperone, atp11p. database searches have identified protein sequences from schizosaccharomyces pombe and two species of drosophila that are homologous to s. cerevisiae atp11p. a cdna encoding the putative atp11p from drosophila yakuba was shown to complement the respiratory deficient phenotype of yeast harboring an atp11::his3 disruption allele. furthermore, the product of this drosophila gene was shown to in ... | 1999 | 10386611 |
| local recombination and mutation effects on molecular evolution in drosophila. | i studied the cause of the significant difference in the synonymous-substitution pattern found in the achaete-scute complex genes in two drosophila lineages, higher codon bias in drosophila yakuba, and lower bias in d. melanogaster. besides these genes, the functionally unrelated yellow gene showed the same substitution pattern, suggesting a region-dependent phenomenon in the x-chromosome telomere. because the numbers of a/t --> g/c substitutions were not significantly different from those of g/ ... | 1999 | 10545459 |
| origin of new genes and source for n-terminal domain of the chimerical gene, jingwei, in drosophila. | this paper deals with a general question posed by the origin of new processed chimerical genes: when a new retrosequence inserts into a new genome position, how does it become activated and acquire novel protein function by recruiting new functional domains and regulatory elements? jingwei (jgw), a newly evolved functional gene with a chimerical structure in drosophila, provides an opportunity to examine such questions. the source of its exon encoding c-terminal peptide has been identified as an ... | 1999 | 10570991 |
| the evolution of an alpha-esterase pseudogene inactivated in the drosophila melanogaster lineage. | previous analyses of the alpha-esterase cluster of drosophila melanogaster revealed 10 active genes and the dmalphae4a-psi pseudogene. here, we reconstruct the evolution of the pseudogene from the sequences of 12 alleles from widely scattered d. melanogaster populations and single alleles from drosophila simulans and drosophila yakuba. all of the dmalphae4a-psi alleles contain numerous inactivating mutations, suggesting that pseudogene alleles are fixed in natural populations. several lines of e ... | 2000 | 10742048 |
| the complete sequence of the mitochondrial genome of the crustacean penaeus monodon: are malacostracan crustaceans more closely related to insects than to branchiopods? | the complete sequence of the mitochondrial genome of the giant tiger prawn, penaeus monodon (arthropoda, crustacea, malacostraca), is presented. the gene content and gene order are identical to those observed in drosophila yakuba. the overall at composition is lower than that observed in the known insect mitochondrial genomes, but higher than that observed in the other two crustaceans for which complete mitochondrial sequence is available. analysis of the effect of nucleotide bias on codon compo ... | 2000 | 10833192 |
| mitochondrial sequence evolution in spiders: intraspecific variation in trnas lacking the tpsic arm. | analyses of mitochondrial dna sequences from three species of habronattus jumping spiders (chelicerata: arachnida: araneae) reveal unusual inferred trna secondary structures and gene arrangements, providing new information on trna evolution within chelicerate arthropods. sequences from the protein-coding genes nadh dehydrogenase subunit 1 (nd1), cytochrome oxidase subunit i (coi), and subunit ii (coii) were obtained, along with trna, trna, and large-subunit ribosomal rna (16s) sequences; these r ... | 2000 | 10889222 |
| evolutionary novelties in islands: drosophila santomea, a new melanogaster sister species from são tomé. | the finding of new melanogaster sister species may help us in understanding more about how the emergence of genetic novelties, particularly in insular habitats, can result in speciation. here we report on the discovery of drosophila santomea, which is the first melanogaster sibling found off west-equatorial africa, on são tomé, one of the gulf of guinea islands. although the eight other melanogaster sister species are remarkably conservative in their morphology except for their terminalia, the n ... | 2000 | 11007323 |
| local changes in gc/at substitution biases and in crossover frequencies on drosophila chromosomes. | i present here evidence of remarkable local changes in gc/at substitution biases and in crossover frequencies on drosophila chromosomes. the substitution pattern at 10 loci in the telomeric region of the x chromosome was studied for four species of the drosophila melanogaster species subgroup. drosophila orena and drosophila erecta are clearly the most closely related species pair (the erecta complex) among the four species studied; however, the overall data at the 10 loci revealed a clear dicho ... | 2001 | 11264413 |
| numerous gene rearrangements in the mitochondrial genome of the wallaby louse, heterodoxus macropus (phthiraptera). | the complete arrangement of genes in the mitochondrial (mt) genome is known for 12 species of insects, and part of the gene arrangement in the mt genome is known for over 300 other species of insects. the arrangement of genes in the mt genome is very conserved in insects studied, since all of the protein-coding and rrna genes and most of the trna genes are arranged in the same way. we sequenced the entire mt genome of the wallaby louse, heterodoxus macropus, which is 14,670 bp long and has the 3 ... | 2001 | 11319269 |
| one-step pcr amplification of complete arthropod mitochondrial genomes. | a new pcr primer set which enables one-step amplification of complete arthropod mitochondrial genomes was designed from two conserved 16s rdna regions for the long pcr technique. for this purpose, partial 16s rdnas amplified with universal primers 16sa and 16sb were newly sequenced from six representative arthropods: armadillidium vulgare and macrobrachium nipponense (crustacea), anopheles sinensis (insecta), lithobius forficatus and megaphyllum sp. (myriapoda), and limulus polyphemus (chelicera ... | 2001 | 11399145 |
| sequence and organization of the mitochondrial genome of the chagas disease vector, triatoma dimidiata. | the 17 019 bp mitochondrial genome of triatoma dimidiata is composed of thirteen protein coding sequences, twenty-two trnas, small and large ribosomal units, and a control region. the gene order and orientation are identical to that of drosophila yakuba. the nucleotide composition is biased toward adenine and thymine (69.5% a + t). the 2.1 kb putative control region, known as the a + t rich region in most insects, has an a + t bias of 66%, but contains a 400 bp sequence that is 77.5% a + t and t ... | 2001 | 11437912 |
| genetics of a difference in pigmentation between drosophila yakuba and drosophila santomea. | drosophila yakuba is a species widespread in africa, whereas d. santomea, its newly discovered sister species, is endemic to the volcanic island of são tomé in the gulf of guinea. drosophila santomea probably formed after colonization of the island by its common ancestor with d. yakuba. the two species differ strikingly in pigmentation: d. santomea, unlike the other eight species in the d. melanogaster subgroup, almost completely lacks dark abdominal pigmentation. d. yakuba shows the sexually di ... | 2002 | 12487356 |
| evolution of gene expression in the drosophila melanogaster subgroup. | little is known about broad patterns of variation and evolution of gene expression during any developmental process. here we investigate variation in genome-wide gene expression among drosophila simulans, drosophila yakuba and four strains of drosophila melanogaster during a major developmental transition--the start of metamorphosis. differences in gene activity between these lineages follow a phylogenetic pattern, and 27% of all of the genes in these genomes differ in their developmental gene e ... | 2003 | 12548287 |
| microevolutionary divergence pattern of the segmentation gene hunchback in drosophila. | to study the microevolutionary processes shaping the evolution of the segmentation gene hunchback (hb) from drosophila melanogaster, we cloned and sequenced the gene from 12 isofemale lines representing wild-type populations of d. melanogaster, as well as from the closely related species drosophila sechellia, drosophila orena, and drosophila yakuba. we find a relatively low degree of sequence variation in d. melanogaster (theta = 0.0017), which is, however, consistent with its chromosomal locati ... | 1998 | 12572604 |
| sexual isolation between two sibling species with overlapping ranges: drosophila santomea and drosophila yakuba. | drosophila yakuba is widespread in africa, whereas d. santomea, its newly discovered sister species, is endemic to the volcanic island of são tomé in the gulf of guinea. drosophila santomea probably formed after colonization of the island by a d. yakuba-like ancestor. the species presently have overlapping ranges on the mountain pico do são tomé, with some hybridization occurring in this region. sexual isolation between the species is uniformly high regardless of the source of the populations, a ... | 2002 | 12583583 |
| complete mitochondrial dna sequence of the swimming crab, portunus trituberculatus (crustacea: decapoda: brachyura). | we determined the complete mitochondrial dna sequence for a swimming crab portunus trituberculatus (miers, 1876) (crustacea: decapoda: brachyura). the entire genome was amplified using a long pcr technique, and the products were subsequently used as templates for direct sequencing using a primer walking strategy. the genome (16,026 bp) contained the same 37 genes (two rrnas, 22 trnas, and 13 proteins) plus the putative control region as found in other arthropods. the gene order of p. tritubercul ... | 2003 | 12853147 |
| an evolutionary analysis of orphan genes in drosophila. | orphan genes are protein-coding regions that have no recognizable homolog in distantly related species. a substantial fraction of coding regions in any genome sequenced consists of orphan genes, but the evolutionary and functional significance of orphan genes is not understood. we present a reanalysis of the drosophila melanogaster proteome that shows that there are still between 26% and 29% of all proteins without a significant match with noninsect sequences, and that these orphans are underrep ... | 2003 | 14525923 |
| what maintains noncytoplasmic incompatibility inducing wolbachia in their hosts: a case study from a natural drosophila yakuba population. | cytoplasmic incompatibility (ci) allows wolbachia to invade hosts populations by specifically inducing sterility in crosses between infected males and uninfected females. in some species, non-ci inducing wolbachia, that are thought to derive from ci-inducing ancestors, are common. in theory, the maintenance of such infections is not possible unless the bacterium is perfectly transmitted to offspring--and/or provides a fitness benefit to infected females. the present study aims to test this view ... | 2004 | 15009266 |
| conspecific sperm precedence in sister species of drosophila with overlapping ranges. | barriers to gene flow that act after mating but before fertilization are often overlooked in studies of reproductive isolation. where species are sympatric, such "cryptic" isolating barriers may be important in maintaining species as distinct entities. drosophila yakuba and its sister species d. santomea have overlapping ranges on the island of são tomé, off the coast of west africa. previous studies have shown that the two species are strongly sexually isolated. however, the degree of sexual is ... | 2004 | 15154554 |
| natural wolbachia infections in the drosophila yakuba species complex do not induce cytoplasmic incompatibility but fully rescue the wri modification. | in this study, we report data about the presence of wolbachia in drosophila yakuba, d. teissieri, and d. santomea. wolbachia strains were characterized using their wsp gene sequence and cytoplasmic incompatibility assays. all three species were found infected with wolbachia bacteria closely related to the wau strain, found so far in d. simulans natural populations, and were unable to induce cytoplasmic incompatibility. we injected wri, a ci-inducing strain naturally infecting d. simulans, into t ... | 2004 | 15238531 |
| sequence turnover and tandem repeats in cis-regulatory modules in drosophila. | the path by which regulatory sequence can change, yet preserve function, is an important open question for both evolution and bioinformatics. the recent sequencing of two additional species of drosophila plus the wealth of data on gene regulation in the fruit fly provides new means for addressing this question. for regulatory sequences, indels account for more base pairs (bp) of change than substitutions (between drosophila melanogaster and drosophila yakuba), though they are fewer in number. us ... | 2005 | 15659554 |
| insect mitochondrial genomics: the complete mitochondrial genome sequence of the meadow spittlebug philaenus spumarius (hemiptera: auchenorrhyncha: cercopoidae). | we present the complete mitochondrial genome sequence of the meadow spittlebug philaenus spumarius (auchenorrhyncha: cercopoidae). this contribution represents the second mitochondrial genome from the hemiptera and the second of the three hemipteran suborders sampled. the genome is a circular molecule of 16 324 bp with a total a+t content of 77.0% and 76.7% for coding regions only. the gene content, order, and structure are consistent with the drosophila yakuba genome structure (clary and wolste ... | 2005 | 15729396 |
| the complete nucleotide sequence and gene organization of the mitochondrial genome of the oriental mole cricket, gryllotalpa orientalis (orthoptera: gryllotalpidae). | the complete nucleotide sequences of the mitochondrial genome (mitogenome) of the oriental mole cricket, gryllotalpa orientalis (orthoptera: gryllotalpidae), were determined. the 15,521-bp-long g. orientalis mitogenome contains typical gene complement, base composition, and codon usage found in metazoan mitogenomes. the g. orientalis mitogenome contains the third lowest a+t content (70.5%) among the complete insects mt genome sequences. the initiation codon for the g. orientalis coi gene appears ... | 2005 | 15950403 |
| a cellular drosophila melanogaster protein with similarity to baculovirus f envelope fusion proteins. | baculovirus f (fusion) proteins are found in the envelopes of budded virions. recently a drosophila melanogaster gene (cg4715) that encodes a protein with sequence similarity to baculovirus f proteins was discovered. to examine similarities and differences with baculovirus f proteins, we cloned the d. melanogaster cellular f (dm-cf) protein gene and analyzed dm-cf expression and localization. the predicted dm-cf protein sequence lacks a furin cleavage site, and transiently expressed dm-cf showed ... | 2005 | 15956544 |
| multilocus analysis of introgression between two sympatric sister species of drosophila: drosophila yakuba and d. santomea. | drosophila yakuba is widely distributed in sub-saharan africa, while d. santomea is endemic to the volcanic island of são tomé in the atlantic ocean, 280 km west of gabon. on são tomé, d. yakuba is found mainly in open lowland forests, and d. santomea is restricted to the wet misty forests at higher elevations. at intermediate elevations, the species form a hybrid zone where hybrids occur at a frequency of approximately 1%. to determine the extent of gene flow between these species we studied po ... | 2005 | 15965264 |
| quantitative trait loci affecting the difference in pigmentation between drosophila yakuba and d. santomea. | using quantitative trait locus (qtl) mapping, we studied the genetic basis of the difference in pigmentation between two sister species of drosophila: drosophila yakuba, which, like other members of the d. melanogaster subgroup, shows heavy black pigmentation on the abdomen of males and females, and d. santomea, an endemic to the african island of são tomé, which has virtually no pigmentation. here we mapped four qtl with large effects on this interspecific difference in pigmentation: two on the ... | 2005 | 15972457 |
| rapid evolution of genomic acp complement in the melanogaster subgroup of drosophila. | unusual properties of molecular evolution in reproduction-related drosophila genes, including atypically rapid rates of protein evolution, support the idea that natural selection plays an important role in divergence of reproductive function in drosophila. we used subtractive hybridization to investigate another potential side of evolution of the male reproductive transcriptome. we carried out a screen for genes with much greater transcript abundance in drosophila simulans reproductive tracts th ... | 2005 | 15987879 |
| sexual isolation and cuticular hydrocarbon differences between drosophila santomea and drosophila yakuba. | drosophila santomea and drosophila yakuba are two sister species inhabiting saõ tomé island. previous studies showed that both species display strong reproductive isolation, although they can produce a few viable hybrids. our study tried to understand the mechanism of this ethological isolation between two allopatric strains. a strong sexual isolation was confirmed, with a marked asymmetry. comparisons of latency times to either courtship or copulation suggest that males do not discriminate fema ... | 2005 | 16132336 |
| recently evolved genes identified from drosophila yakuba and d. erecta accessory gland expressed sequence tags. | the fraction of the genome associated with male reproduction in drosophila may be unusually dynamic. for example, male reproduction-related genes show higher-than-average rates of protein divergence and gene expression evolution compared to most drosophila genes. drosophila male reproduction may also be enriched for novel genetic functions. our earlier work, based on accessory gland protein genes (acp's) in d. simulans and d. melanogaster, suggested that the melanogaster subgroup acp's may be lo ... | 2006 | 16361246 |
| genomewide comparative analysis of the highly abundant transposable element dine-1 suggests a recent transpositional burst in drosophila yakuba. | dine-1 (drosophila interspersed element) is the most abundant repetitive sequence in the drosophila genome derived from transposable elements. it comprises >1% of the drosophila melanogaster genome (dmg) and is believed to be a relic from an ancient transpositional burst that occurred approximately 5-10 mya. we performed a genomewide comparison of the abundance, sequence variation, and chromosomal distribution of dine-1 in d. melanogaster and d. yakuba. unlike the highly diverged copies in the d ... | 2006 | 16387876 |
| x chromosomes and autosomes evolve at similar rates in drosophila: no evidence for faster-x protein evolution. | recent data from drosophila suggest that a substantial fraction of amino acid substitutions observed between species are beneficial. if these beneficial mutations are on average partially recessive, then the rate of protein evolution is predicted to be faster for x-linked genes compared to autosomal genes (the "faster-x" hypothesis). we test this prediction by comparing rates of protein substitutions between orthologous genes, taking advantage of variations in chromosome fusions within the genus ... | 2006 | 16520459 |
| an anomalous hybrid zone in drosophila. | despite the genetic tractability of many of drosophila species, the genus has few examples of the "classic" type of hybrid zone, in which the ranges of two species overlap with a gradual transition from one species to another through an area where hybrids are produced. here we describe a classic hybrid zone in drosophila that involves two sister species, drosophila yakuba and d. santomea, on the island of são tomé. our transect of this zone has yielded several surprising and anomalous findings. ... | 2005 | 16526507 |
| strong regional heterogeneity in base composition evolution on the drosophila x chromosome. | fluctuations in base composition appear to be prevalent in drosophila and mammal genome evolution, but their timescale, genomic breadth, and causes remain obscure. here, we study base composition evolution within the x chromosomes of drosophila melanogaster and five of its close relatives. substitutions were inferred on six extant and two ancestral lineages for 14 near-telomeric and 9 nontelomeric genes. gc content evolution is highly variable both within the genome and within the phylogenetic t ... | 2006 | 16547109 |
| extensive introgression of mitochondrial dna relative to nuclear genes in the drosophila yakuba species group. | studies of gene flow between recently diverged species can illuminate the role of natural selection in the formation of new species. drosophila santomea and d. yakuba are recently diverged, partially reproductively isolated species that continue to hybridize in the wild, and appear to be reproductively isolated from the more distantly related species d. teissieri. we examine patterns of nucleotide polymorphism and divergence in these three species at multiple x-linked, y-linked, and mitochondria ... | 2006 | 16610321 |
| fast protein evolution and germ line expression of a drosophila parental gene and its young retroposed paralog. | this is the first detailed study of the evolution, phylogenetic distribution, and transcription of one young retroposed gene, cg13732, and its parental gene cg15645, whose functions are unknown. cg13732 is a recognizable retroposed copy of cg15645 retaining the signals of this process. we name the parental gene cervantes and the retrogene quijote. to determine when this duplication occurred and the phylogenetic distribution of quijote, we employed polymerase chain reaction, southern blotting, an ... | 2006 | 16916943 |
| intron length evolution in drosophila. | i present data on the evolution of intron lengths among 3 closely related drosophila species, d. melanogaster, drosophila simulans, and drosophila yakuba. using d. yakuba as an outgroup, i mapped insertion and deletion mutations in 148 introns (spanning approximately 30 kb) to the d. melanogaster and d. simulans lineages. intron length evolution in the 2 sister species has been different: in d. melanogaster, x-linked introns have increased slightly in size, whereas autosomal ones have decreased ... | 2006 | 16923822 |
| genetic interactions between the parasitoid wasp leptopilina boulardi and its drosophila hosts. | coevolutionary arms races between hosts and parasites would not occur without genetic variation for traits involved in the outcome of parasitism. genetic variations in resistance and virulence have only rarely been described in pairwise host-parasitoid interactions and have never been analysed in multi-species interactions, in contrast to well-characterized plant-pathogen interactions. this paper reports genetic variation in resistance of drosophila yakuba to the parasitoid wasp leptopilina boul ... | 2007 | 17035953 |
| maximum likelihood estimation of ancestral codon usage bias parameters in drosophila. | we present a likelihood method for estimating codon usage bias parameters along the lineages of a phylogeny. the method is an extension of the classical codon-based models used for estimating dn/ds ratios along the lineages of a phylogeny. however, we add one extra parameter for each lineage: the selection coefficient for optimal codon usage (s), allowing joint maximum likelihood estimation of s and the dn/ds ratio. we apply the method to previously published data from drosophila melanogaster, d ... | 2007 | 17041152 |
| phylogenetic incongruence in the drosophila melanogaster species group. | drosophila melanogaster and its close relatives are used extensively in comparative biology. despite the importance of phylogenetic information for such studies, relationships between some melanogaster species group members are unclear due to conflicting phylogenetic signals at different loci. in this study, we use twelve nuclear loci (eleven coding and one non-coding) to assess the degree of phylogenetic incongruence in this model system. we focus on two nodes: (1) the node joining the drosophi ... | 2007 | 17071113 |
| comparative genomic analysis of allatostatin-encoding (ast) genes in drosophila species and prediction of regulatory elements by phylogenetic footprinting. | the role of the yxfgla family of allatostatin (ast) peptides in dipterans is not well-established. the recent completion of sequencing of genomes for multiple drosophila species provides an opportunity to study the evolutionary variation of the allatostatins and to examine regulatory elements that control gene expression. we performed comparative analyses of ast genes from seven drosophila species (drosophila melanogaster, drosophila simulans, drosophila ananassae, drosophila yakuba, drosophila ... | 2007 | 17175069 |
| evidence for de novo evolution of testis-expressed genes in the drosophila yakuba/drosophila erecta clade. | the mutational origin and subsequent evolution of de novo genes, which are hypothesized to be genes of recent origin that are not obviously related to ancestral coding sequence, are poorly understood. however, accumulating evidence suggests that such genes may often function in male reproduction. here we use testis-derived expressed sequence tags (ests) from drosophila yakuba to identify genes that have likely arisen either in d. yakuba or in the d. yakuba/d. erecta ancestor. we found several su ... | 2007 | 17435230 |
| the drosulfakinin 0 (dsk 0) peptide encoded in the conserved dsk gene affects adult drosophila melanogaster crop contractions. | we report that the drosulfakinin 0 (dsk 0; nqktmsfnh2) structure and genomic organization are conserved. the dsk 0 c-terminus, sfnh2, is widely distributed in the animal kingdom suggesting it defines a novel peptide family. we also report the first description of dsk 0 activity. dsk 0, i (dsk i, fddyghmrfnh2), and ii (dsk ii, ggddqfddyghmrfnh2) are encoded in sulfakinin (dsk). drosophila erecta, drosophila sechellia, drosophila simulans, and drosophila yakuba shared 62.5-87.5% identity to drosop ... | 2007 | 17632121 |
| adaptive protein evolution of x-linked and autosomal genes in drosophila: implications for faster-x hypotheses. | patterns of sex chromosome and autosome evolution can be used to elucidate the underlying genetic basis of adaptative change. evolutionary theory predicts that x-linked genes will adapt more rapidly than autosomes if adaptation is limited by the availability of beneficial mutations and if such mutations are recessive. in drosophila, rates of molecular divergence between species appear to be equivalent between autosomes and the x chromosome. however, molecular divergence contrasts are difficult t ... | 2007 | 17884828 |