Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cytochemical studies on rnp complexes produced by puff 2-48bc in drosophila hydei: uranyl acetate and phosphotungstic acid staining. | differential staining of the core and rnp particles of rnp complexes in puff 2--48 bc in salivary gland chromosomes of drosophila hydei was achieved with aqueous uranyl acetate (ua) at low ph, with ua in acetone, with phosphotungstic acid (pta) in organic solvents, and with aqueous pta at ph 5 and 6. a comparison of the results of ua and pta staining under various conditions indicate that the proteins in the core region and in the rnp particles connected to it differ with respect to their amino- ... | 1976 | 56256 |
| selective staining of y chromosomal loops in drosophila hydei, d. neohydei, and d. eohydei. | modified giemsa procedures have been developed which elicit differential and highly selective staining of individual y chromosomal lampbruch loops in spermatocyte nuclei of drosophila hydei, d. neohydei, and d. eohydei. in all three species the y loop pair known as the "clubs" stains a brilliant dark red with giemsa at ph 10. with the same treatment other loop pairs either remain unstained, e.g. the "threads", or show a differentiation between light blue and pink staining material, e.g. "pseudon ... | 1977 | 67013 |
| purification of mitochondrial nadh dehydrogenase from drosophila hydei and comparison with the 'heat-shock' polypeptides. | mitochondrial nadh dehydrogenase (nadh:(acceptor) oxidoreductase, ec .6.99.3) from either drosophila hydei larvae or embryos has been purified 150- and 120-fold, respectively. the purified enzyme appeared homogeneous and showed a molecular weight of 57 000. the molecular weight of the nondenatured enzyme was 79 000. on isoelectro-focussing of the preparation, two fractions were observed, a major one with an isoelectric point of 6.2 and a minor fraction with an isoelectric point of 4.9. straight- ... | 1978 | 222325 |
| indirect immunofluorescence microscopy of microtubular structures in male germ cells of wildtype and l(3)pl (lethal-polyploid) drosophila hydei. | tubulin-containing structures of the male germ cells of drosophila hydei crossreact in indirect immunofluorescence microscopy with antibody directed against homogeneous porcine brain tubulin. there is no detectable difference in reactivity between germ cells of wildtype flies and the mutant l(3)pl (lethal-polyploid) which is characterized by microtubular abnormalities. however, the technique of indirect immunofluorescence microscopy allows the direct visualization of several abnormalities in the ... | 1978 | 350682 |
| the activity of two heat shock loci of drosophila hydei in tissue culture cells and salivary gland cells as analyzed by in situ hybridization of complementary dna. | complementary dna was made to poly a+ nuclear or polysomal rna isolated from heat shock tissue culture cells of drosophila hydei. a number of loci other than the four major heat shock loci are labelled after in situ hybridization of these cdna preparations, while solution hybridization indicated that only about 10% of the cdna was specific for heat shocked cells. removal of the fraction of cdna which could react with 25 degrees c rna and subsequent in situ hybridization of heat shock specific cd ... | 1979 | 467169 |
| lengths of transcribed rdna repeating units in spermatocytes of drosophila hydei: only genes without an intervening sequence are expressed. | in drosophila hydei a 5 kb long intervening sequence (ivs) which is limited to only one of the three nucleolus organizers occurs in the 28s coding region of the rdna (kunz and glätzer, 1979). this raises the possibility to study transcription of repeated genes with long intervening sequences. three genotypes of males were studied each having only one defined nucleolus organizer. spermatocytes of single males were spread in a solution of low salt and examined by electron microscopy. the length di ... | 1979 | 533667 |
| a procedure for the cloning and identification of y-specific middle repetitive sequences in drosophila hydei. | 1979 | 537053 | |
| morphogenetic deficiencies in transplanted gonadal anlagen of the mutant l(3)pl (lethal-polyploid) in drosophila hydei. | 1977 | 559578 | |
| abnormal microtubules in testes of the mutant l(3)pl (lethal-polyploid) of drosophila hydei, cultured in vivo. | 1977 | 559579 | |
| dna distribution and intrachromosomal organization of moderately repetitive sequences. | the dna content of individual subregions along the 4th chromosome of drosophila hydei has been measured. 51% of the subregions have a dna content averaging 0.7-0.8 pg; 31% a mean dna content of 0.25-0.35 pg and 18% a mean dna content of 1.7 pg. moreover the structural chromosomal distribution of moderately repetitive dna is not random since the specific activity of the chromosomal segments in terms of those sequences is not the same. 9% of the subregions are very poor in repetitive sequences and ... | 1977 | 610873 |
| in situ hybridization of nuclear and cytoplasmic rna to locus 2-48bc in drosophila hydei. | the maximum grain density over the "heat-shock" locus 2-48bc of drosophila hydei polytene chromosomes obtained after in situ hybridization of nuclear rna extracted from tissue culture cells labelled during incubation at 37 degrees c is five times higher than that obtainable by using polysomal rna isolated from the same cells. furthermore, the addition of a large excess of unlabelled polysomal rna reduced the amount of in situ hybridization of nuclear rna by only 20% showing that nuclear 2-48bc r ... | 1978 | 630996 |
| the products of the "heat-shock" loci of drosophila hydei. correlation between locus 2-36a and the 70,000 mw "heat-shock" peptide. | antibody was raised against total drosophila hydei embryonic cellular protein with a molecular weight between 65,000 and 70,000 dalton. this antiserum reacted with the 70,000 mw "heat-shock" peptide found in "s labelled cell extracts of "heat-shocked" d. hydei tissue culture cells or salivary glands.--the antibody was coupled to sepharose 4b and this material was used to absorb polysomes obtained from tissue culture cells incubated at 37 degrees c in the presence of tritiated rna precursors. the ... | 1978 | 639626 |
| heat-shock peptides in drosophila hydei and their synthesis in vitro. | 1978 | 689021 | |
| induced rnp production in different cell types of drosophila. | the typical rnp complexes produced in puff 2-48bc in the salivary gland chromosomes as a consequence of treatments interfering with the cellular respiratory metabolism have been found in various other cell types after treatment with 5-10- minus 2 m vitamin b6 for 4 hr. in drosophila hydei the typical rnp complexes were present in puff 2-48bc in the nuclei of gastric caeca cells, in nuclei of cells of the imaginal (wing) discs and embryonic cells in primary culture. similar rnp complexes were obs ... | 1975 | 806351 |
| the location of the nucleolus organizer regions in drosophila hydei. | the positions of the nucleolus organizer regions in metaphase chromosomes of drosophila hydei were detected by in situ hybridization experiments. in agreement with earlier conclusions the nucleolus of the x chromosome was found to originate in a terminal region of the heterochromatic arm. the y chromosome contains two nucleolus organizers, one in a terminal postion of the long arm, and the other in the short arm. the implications with respect to the evolution of the y chromosome are discussed. | 1975 | 806438 |
| variations in the number of the y chromosomal rrna genes in drosophila hydei. | the number of rrna cistrons is measured by filter saturation hybridization in different stocks of d. hydei, where the wild-type x chromosome has one nucleolus organizer (no) and wild-type y has two separated no's. xx/y females having no x chromosomal no show an rdna content exceeding that of a y chromosome. an even greater increase in the rrna cistron number is measured in two translocation stocks where the xx is combined with one half of a y and, therefore, each stock contains only one of the t ... | 1976 | 814041 |
| mutation, recombination and genetic organization in drosophila hydei, compared to other drosophila species. | 2010 | 829893 | |
| chromosomal puff induction in salivary glands from drosophila hydei by arsenite. | 1977 | 913493 | |
| size and distribution of polyadenylic acid sequences in drosophila polytene dna and rna. | [3h]poly(u) hybridizes very rapidly to polytene dna from drosophila hydei. when hybridization is performed at 30 degrees c in 2 x ssc to a large excess of dna, 95% of the poly(u) becomes ribonuclease resistant. also, complementary rna transcribed in vitro from polytene dna hybridizes to poly(u). 023--0.25% of the dna is composed of (da)-rich sequences and 0.23--0.31% of crna hybridizes to [3h]poly(u). the length of the (da)-rich sequences on the dna and crna is 40 nucleotides. the tm values of t ... | 1977 | 921997 |
| rna synthesis in puff 2-48bc after experimental induction in drosophila hydei. | puffs induced by treatment with vitamin b6 at locus 2-48bc in salivary gland cells of drosophila hydei were isolated by micromanipulation, and the puff rna was analyzed electrophoretically. the main rna species migrated with a mobility of 40s. a similar rna species was found in this puff after its induction by temperature treatment, except that in this case material with a higher mobility was also found, presumably due to the presence of growing chains. no evidence was found for polyadenylation ... | 1976 | 971486 |
| ribonuclear protein formation at locus 2-48 bc in drosophila hydei. | 1976 | 972687 | |
| protein synthesis in salivary glands of drosophila hydei after experimental gene induction. | several treatments, namely incubation at 37 degrees c, in the presence of arsenite, 2,4-dinitrophenol or vitamin b-6, or release from anaerobiosis induce the same set of puffs in the polythene chromosomes of salivary glands of drosophila hydei. analysis of changes in protein-synthetic patterns (as determined by radioautography of sodium dodecyl sulphate-gel electrophoretograms of extracts from [35s]methionine-labelled salivary glands) showed that concomitant with puff induction by these various ... | 1976 | 985453 |
| rna synthesis with giant chromosomes of isolated drosophila hydei nuclei as a correlate to the experimentally altered chromosome morphology. | 1976 | 991944 | |
| a differential effect of the incubation temperature on the inhibition of rna synthesis by drb in cells of drosophila hydei. | 1976 | 992058 | |
| replication patterns in polytene chromosomes of drosophila hydei, drosophila neohydei and their hybrids. | 2006 | 1053154 | |
| ribosomal dna content and bobbed phenotype in drosophila hydei. | the number of ribosomal rna genes in different drosophila hydei stocks has been determined by filter saturation hybridisation experiments. it has been shown that there is no marked correlation between the average rrna gene number per cell in the whole animal and the bobbed phenotype when y chromosomal nucleolar organisers are present. | 1976 | 1070484 |
| the proteins of polytene chromosomes of drosophila hydei. | it is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. it is suggested that these proteins are important components of constitutive heterochromatin, which is severely underrepresented in polytene chromosomes. chromosome morphology, including the pattern of induced puffs, is maintained ... | 1975 | 1095320 |
| the submicroscopic structure of synthetically active units in a puff of drosophila hydei giant chromosomes. | thin sections of nuclei of salivary glands of drosophila hydei, treated in vitro with vitamin b-6 in the presence of either actinomycin d or 3'deoxyadenosine revealed the morphology of transcription units observed display a definite polarity with respect to the size of the rnp-particles attached to the deoxynucleoprotein matrix. the length of the longest unit observed was approximately 1.5 micronm, which corresponds well with the size of the rna extracted from isolated puffs and with preliminary ... | 1975 | 1132284 |
| absence of cell communication for fluorescein and dansylated amino acids in an electrotonic coupled cell system. | quantitative evaluation of the diffusion process of sodium fluorescein and dansylated amino acids in the salivary gland of the larvae of drosophila hydei reveals that the differences in specific permeability between the junctional and nonjunctional membranes, as found for small ions, do not apply to the fluorescent probes. there are no significant differences between the permeability properties for the different dansylated amino acids tested, and the same properties are found for sodium fluoresc ... | 1975 | 1148233 |
| the location of 5s (ribosomal) rna genes in drosophila hydei. | the location of the 5s ribosomal rna cistrons in band 2-23b1,2 of the polytene (salivary gland) chromosomes of drosophila hydei was indicated by in situ hybridization of tritiated low molecular weight rna fractionated from total in vivo synthesized larval rna or from in vitro synthesized salivary gland rna and competition of the hybridization of this rna by 5s rna obtained from calf lens ribosomes. -- at the submicroscopic level, band 2-23b1,2 in salivary gland chromosomes shows a compact organi ... | 1975 | 1175453 |
| two separated nucleolus organizers on the drosophila hydei y chromosome. | by genetical, cytological, and filter saturation hybridization methods it is shown that the y chromosome of drosophila hydei contains two separate nucleolus organizers, one on the short arm, the second near the tip of the long arm. | 1975 | 1186660 |
| independent replication of the ribosomal rna genes in the polytrophic-meroistic ovaries of calliphora erythrocephala, drosophila hydei, and sarcophaga barbata. | by filter saturation hybridizations the ribosomal (r)dna contents of the ovaries calliphora erythrocephala, drosophila hydei, and sarcophaga barbata have been measured in comparison to the rdna percentages of their diploid brains. the measurements of the ovarian rdna have been carried out on ovaries where the nurse cells in the distal egg chamber of the ovarioles had reached their highest ploidy level. the diploid rdna content of each of the respective species was chosen as a 100% standard and t ... | 1975 | 1201686 |
| induced transcription-dependent synthesis of mitochondrial reduced nicotinamide-adenine dinucleotide dehydrogenase in drosophila. | salivary glands of drosophila hydei recovering from an anaerobic treatment show a significant increase in apparent vmax of mitochondrial nadh dehydrogenase. this increase in vmax is based on an increase in enzyme molecules resulting from synthesis de novo in the cytoplasm, as indicated by the inhibition by cycloheximide of both the increase in apparent vmax and the increase in amino acid incorporation into enzyme fractions. the increase in enzyme activity is also inhibited by actinomycin d, whic ... | 1975 | 1212223 |
| visualization of gene transcription in spermatocytes of drosophila hydei. | x chromosomal and/or autosomal transcriptional activity has been visualized by electron microscopy in primary spermatocyte nuclei of drosophila hydei lacking a y chromosome. the spreading technique applied uses a mixture of a hypotonic medium plus a detergent to disperse single germ cells. no effect of the detergent on the quality of the spread was detected using spread rrna cistrons as a reference. the intervals between the transcripts are highly variable ranging from 0.1 mum to more than 3 mun ... | 1975 | 1212903 |
| intragenic recombination at the white locus of drosophila hydei. | a fine-structure analysis of the white locus in drosophila hydei was carried out by means of allele recombination. four mutants, derived from wild type, mapped at three subloci. these are possibly homologous to the main subloci 2, 3, and 4 of d. melanogaster. three secondary mutants, derived from the primary wiv allele, were located in the proximal part of the gene. one of them appeared as a homoallele of the original wiv, whereas the remaining two are better explained either as double mutants o ... | 1975 | 1214776 |
| new compound (1) chromosomes and the production of large quantities of x/o males in drosophila hydei. | 1975 | 1225764 | |
| minute-type mutants in drosophila hydei. | 1975 | 1235049 | |
| characterization of the reverse transcriptase of 1731, a drosophila melanogaster retrotransposon. | the nucleotide sequence of 1731, a retrotransposon cloned from the genome of drosophila melanogaster, reveals a structural similarity with the proviral form of the retroviruses including a pol-like gene containing a putative reverse-transcriptase(rt)-coding sequence. diverse parts of that sequence were subcloned and expressed in escherichia coli. it has been demonstrated that the expression of the rt-like sequence, when translated, gives rise to peptides displaying enzyme activity characteristic ... | 1992 | 1385119 |
| laminin in the male germ cells of drosophila. | to study genes that may be crucial for the male germ cell development of drosophila we screened a cdna expression library with a polyclonal antiserum against testis proteins of drosophila hydei. we identified a cdna fragment that exhibited a complete sequence similarity with the cdna of the laminin b2 chain, an important component of the extracellular matrix. transcripts of laminin b2 were detected in the rna of male germ cells with the polymerase chain reaction and by in situ hybridization. we ... | 1992 | 1429843 |
| towards a physical map of the fertility genes on the heterochromatic y chromosome of drosophila hydei: families of repetitive sequences transcribed on the lampbrush loops nooses and threads are organized in extended clusters of several hundred kilobases. | the understanding of structure and function of the so-called fertility genes of drosophila is very limited due to their unusual size--several megabases--and their location on the heterochromatic y chromosome. since mapping of these genes has mainly been done by classical cytogenetic analyses using a small number of cytologically visible lampbrush loops as the sole markers for particular fertility genes, the resolution of the genetic map of the y chromosome is restricted to 3-5 mb. here we demons ... | 1992 | 1465096 |
| the evolution of duplicate glyceraldehyde-3-phosphate dehydrogenase genes in drosophila. | in drosophila melanogaster there are two genes which encode the enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh), gapdh-43e and gapdh-13f. we have shown that gapdh-43e codes for the gapdh subunit with an apparently larger molecular weight while gapdh-13f encodes the gapdh subunit having an apparently smaller molecular weight. immunoblots of sodium dodecyl sulfate gels were used to survey species from throughout the genus and results indicated that two classes of gapdh subunits are present ... | 1992 | 1468631 |
| interspecific comparison of the transformer gene of drosophila reveals an unusually high degree of evolutionary divergence. | the transformer (tra) gene of drosophila melanogaster occupies an intermediate position in the regulatory pathway controlling all aspects of somatic sexual differentiation. the female-specific expression of this gene's function is regulated by the sex lethal (sxl) gene, through a mechanism involving sex-specific alternative splicing of tra pre-mrna. the tra gene encodes a protein that is thought to act in conjunction with the transformer-2 (tra-2) gene product to control the sex-specific process ... | 1992 | 1592233 |
| microdeletions in interval 6 of the y chromosome of males with idiopathic sterility point to disruption of azf, a human spermatogenesis gene. | for males with idiopathic sterility, a molecular screen specific for small lesions (microdeletions) in interval 6 of the y chromosome was set up using 29 y-dna probes. a "de novo" microdeletion in y interval 6 was detected in 2 out of 19 "chromosomally normal" sterile males. the first microdeletion includes the y-dna probes py6hp35 and 12f3; the second microdeletion includes the y-dna probes py6hp52, 49f, fr15-ii and the subinterval "c" of probe 50f2. a probe of the py6h sequence family is prese ... | 1992 | 1634226 |
| minos, a new transposable element from drosophila hydei, is a member of the tc1-like family of transposons. | 1991 | 1661410 | |
| spermatogenesis in drosophila. a genetic approach to cellular and subcellular differentiation. | y chromosomal fertility genes are essential for spermatogenesis, but those genes which code for major structural components of the spermatozoon and those controlling sperm morphogenesis must be located on a different chromosome. in the past, it had been questioned whether it would be possible to achieve a meaningful classification of male sterile mutations by light microscopy. i now show, however, that comparison of 244 autosomal male sterile mutants of drosophila hydei with 400 similar mutants ... | 1991 | 1802704 |
| electron microscopic band-interband pattern of polytene chromosomes in drosophila nasuta albomicans. 2. salivary gland chromosome 2l. | the band-interband pattern (division 28-52) of salivary gland chromosome 2l in drosophila nasuta albomicans was studied by light (lm) and electron microscopy (em) using squash preparations and surface-spread polytene (ssp) chromosome preparations, respectively. lm and em maps were complied. based on the digitized em patterns of five homologous ssp chromosomes a computerized em chromosome map was plotted. the em pattern analysis showed a total number of 479 chromosome bands with an almost 83% inc ... | 1991 | 1807929 |
| a potential z-dna-forming sequence is located between two transcription units alternatively expressed during development of drosophila hydei. | recent studies have demonstrated that z-dna exists in vivo in escherichia coli as well as in drosophila and mammalian cells. in the present paper, we show the existence in vivo of z-dna epitopes in the developmentally regulated subregion 4-75c of polytene chromosomes in drosophila hydei. the z-dna epitopes were detected in subdivision c2 only during late third instar when the transcriptional activity of the locus was high. accumulation of nonhistone chromosomal proteins in that locus was also de ... | 1991 | 1840694 |
| binding affinity of leucine-containing chromatin proteins to the polytene x chromosome of drosophila hydei and its significance. | association of newly synthesised non-histone chromosomal protein to the polytene x chromosome of larval salivary glands of d. hydei has been examined by autoradiographic procedure using 3h-leucine. it has been observed that 3h-leucine labelling pattern is inhibited in presence of puromycin. results further reveal that there is a reasonable concordance between the binding affinity of newly synthesized protein on the single x chromosome of the male and paired x's of the female. a sitewise analysis ... | 1991 | 1874548 |
| the alpha regulatory subunit of the mitochondrial f1-atpase complex is a heat-shock protein. identification of two highly conserved amino acid sequences among the alpha-subunits and molecular chaperones. | the recent identification of the alpha-subunit of mitochondrial f1-atpase complex in rat liver peroxisomes suggests another functional role for this protein in both organelles in addition to its involvement in mitochondrial oxidative phosphorylation. we report here that a very rapid response (15 min) in the induction of the alpha-regulatory subunit of the mitochondrial f1-atpase complex is observed in 37 degrees c heat-shocked larvae of drosophila hydei. under the same heat-shock treatment, a si ... | 1990 | 1970818 |
| selection of dna sequences from interval 6 of the human y chromosome with homology to a y chromosomal fertility gene sequence of drosophila hydei. | an experimental approach towards the molecular analysis of the male fertility function, located in interval 6 of the human y chromosome, is presented. this approach is not based on the knowledge of any gene product but on the assumption that the functional dna structure of male fertility genes, evolutionary conserved with their position on the y chromosome, may contain an evolutionary conserved frame structure or at least conserved sequence elements. we tested this hypothesis by using dhmif1, a ... | 1991 | 1999335 |
| characterization of the structure and evolution of the adh region of drosophila hydei. | drosophila of the repleta group have a duplication of the gene which encodes alcohol dehydrogenase (adh). we report the nucleotide sequence of an 8.4-kb region of genomic dna of drosophila hydei which includes the entire adh region. analysis of this sequence reveals similarity in organization to the adh region of drosophila mojavensis and drosophila mulleri of the mulleri subgroup, with three genes ordered 5' to 3', adh-psi, adh-2, adh-1. deletion of a nucleotide in the second codon of each pseu ... | 1991 | 2004708 |
| during drosophila spermatogenesis beta 1, beta 2 and beta 3 tubulin isotypes are cell-type specifically expressed but have the potential to coassemble into the axoneme of transgenic flies. | alpha and beta tubulins exist in a number of different isotypes with distinct expression patterns during development. we have shown by immunofluorescent staining that beta 1, beta 2 and beta 3 tubulins are distributed very specifically in the testes of drosophila. beta 3 tubulin is present exclusively in cytoplasmic microtubules of cells somatic in origin, while the beta 1 isotype is localized in the somatic cells and in early germ cells of both the microtubules of the cytoskeleton as well as in ... | 1991 | 2032541 |
| genetic and cytogenetic analysis of the "th-ps" region of the y chromosome of drosophila hydei: evidence for dual functions of the lampbrush loop-forming fertility genes? | two competing hypotheses have been proposed for the function of the y chromosomal fertility factors in drosophila, which form giant lampbrush loops during the primary spermatocyte stage. the first hypothesis suggests a conventional coding function, the second proposes an unconventional gene function mediated through protein binding by nascent transcripts. therefore, we studied the genetics and cytogenetics of the two y chromosomal fertility genes a and c of drosophila hydei (which form the lampb ... | 1991 | 2062310 |
| mass dense vacuoles in drosophila malpighian tubules contain zinc, not sodium. a reinvestigation by x-ray microanalysis of cryosections. | the intracellular storage of zinc in malpighian tubules of drosophila hydei was studied by x-ray microanalysis of freeze-dried cryosections. mass dense vacuoles in the proximal region of the anterior larval malpighian tubule cells were found to accumulate zinc, not sodium. the zinc content was enhanced considerably after addition of zinc to the food of the larvae. zinc-containing vacuoles were also found after pupation. after starvation of larvae in sea water, na was detected in these vacuoles i ... | 1990 | 2081540 |
| conservatism of sites of trna loci among the linkage groups of several drosophila species. | the sites of seven trna genes (arg-2, lys-2, ser-2b, ser-7, thr-3, thr-4, val-3b) were studied by in situ hybridization. 125i-labeled trna probes from drosophila melanogaster were hybridized to spreads of polytene chromosomes prepared from four drosophila species representing different evolutionary lineages (d. melanogaster, drosophila hydei, drosophila pseudoobscura, and drosophila virilis). most trna loci occurred on homologous chromosomal elements of all four species. in some cases the number ... | 1990 | 2107332 |
| isolation and characterization of a drosophila hydei histone dna repeat unit. | histone genes in d. hydei are organized in tandemly repeated clusters., accomodating in total 120-140 repeat units. we cloned one of the repeat units and analysed the nucleotide sequence. the repeat unit has a size of 5.1 x 10(3) base-pairs and contains one copy of each of the genes coding for the core histones and one copy coding for the histone h1. in the promoter regions of the genes we identified the presumptive cap sites and tata boxes. two additional sequence elements are shared by all fiv ... | 1990 | 2109309 |
| poly[d(c-a)].poly[d(g-t)] is highly transcribed in the testes of drosophila hydei. | microdissection of the lampbrush loops "threads" and "pseudonucleolus" of y chromosomes from primary spermatocytes of drosophila hydei and subsequent microcloning of the dna yielded several recombinant dna clones which cross-hybridized in screening the different clone banks. by dna sequencing we found that the inserts of these cross-hybridizing clones contain blocks of poly[d(c-a].poly[d(g-t)]. testis rna contains a large fraction of transcripts with this simple repeated nucleotide sequence. wit ... | 1990 | 2129287 |
| dna sequence comparison of micropia transposable elements from drosophila hydei and drosophila melanogaster. | members of the retrotransposon family micropia were discovered as constituents of wild-type y chromosomal fertility genes from drosophila hydei. several members of the micropia family have subsequently been recovered from drosophila melanogaster and four micropia elements, micropia-dhmif2, -dhmif8, -dm11 and -dm2, two each from d. hydei and d. melanogaster, have been totally sequenced (17 kb of micropia sequences and 6.8 kb from insertions). comparative analysis of micropia sequences revealed a ... | 1990 | 2162752 |
| regulation of the segmentation gene fushi tarazu has been functionally conserved in drosophila. | an evolutionary approach was applied to identify elements involved in the regulation of the segmentation gene fushi tarazu (ftz) by comparing the drosophila melanogaster ftz gene with its drosophila hydei homologue. the overall organization of the ftz gene is very similar in both species. surprisingly, ftz proved to be inverted in the ant-c of d. hydei with respect to d. melanogaster. strong homologies extend over the entire 6 kb of the ftz upstream region with the best match in the 'upstream el ... | 1990 | 2174353 |
| the heat shock protein hsp70 binds in vivo to subregions 2-48bc and 3-58d of the polytene chromosomes of drosophila hydei. | multiple interactions of members of the hsp70 family with cellular components have already been described. we present, however, the first evidence that upon heat shock treatment hsp70 molecules interact with specific chromosomal subdivisions of the polytene chromosomes of drosophila hydei. after a heat shock treatment of 20 min the protein binds to subdivision 3-58d1 and to the heat shock inducible subdivisions 2-48b3-6 and 2-48c1-2. hsp70 molecules were also observed in subdivision 3-58d1 durin ... | 1990 | 2265568 |
| spermatogenesis of drosophila hydei. | 2008 | 2289847 | |
| oxidized p-phenylenediamine: observations on the staining reaction in epoxy embedded tissues. | p-phenylenediamine (ppd) is easily oxidized to brown compounds which stain acidic substrates. on account of the spontaneous oxidation process, the colour of ppd increases and becomes ochre-brown in a few days, showing an absorption peak at lambda = 510 nm with shoulder at about 440 to 460 nm. studies on the application of oxidized ppd as a stain for semi-thin sections revealed that some tissue components could be clearly visualized. after glutaraldehyde fixation, semi-thin and thin sections of a ... | 1986 | 2422692 |
| structure and function of y chromosomal dna. ii. analysis of lampbrush loop associated transcripts in nuclei of primary spermatocytes of drosophila hydei by in situ hybridization using asymmetric rna probes of four different families of repetitive dna. | psp64/65 subclones of four different families of repetitive sequences on the y chromosome of drosophila hydei were used for in vitro synthesis of labelled rna. pairs of rna probes of opposite strand polarity were employed to analyse rnas transcribed on, or associated with, various y chromosomal lampbrush loops in nuclei of primary spermatocytes of d. hydei. the results of rna filter analysis and in situ hybridization experiments can be generalized as follows: (1) y-specific transcripts are heter ... | 1988 | 2450723 |
| retrotransposon-like sequences are expressed in y chromosomal lampbrush loops of drosophila hydei. | the dna sequence family micropia consists of repeated dna sequences that occur dispersed in the genome of drosophila hydei. members of this dna sequence family were recovered from two recombinant dna clone banks obtained by microdissection of the two y chromosomal lampbrush loop threads and pseudonucleolus from primary spermatocyte nuclei. nucleotide sequence analysis of two of the recombinant dna clones revealed homology to the dna region coding for a reverse transcriptase-like protein in retro ... | 1988 | 2463366 |
| micropia: a retrotransposon of drosophila combining structural features of dna viruses, retroviruses and non-viral transposable elements. | the retrotransposon micropia was first described from y-chromosomal fertility genes of drosophila hydei. screening a drosophila melanogaster genomic library yielded several clones representing micropia elements in d. melanogaster. the dna sequences of two elements from d. hydei (micropia-dhmif2 and micropia-dhmif8) and two elements from d. melanogaster (micropia-dm2 and micropia-dm11) permitted a detailed analysis of the spatial organization of micropia constituents. micropia represents the typi ... | 1988 | 2464689 |
| y chromosome loops in drosophila melanogaster. | primary spermatocyte nuclei of fixed testes of drosophila melanogaster exhibit three large clusters of thread-like structures, each consisting of two long, continuous, loop-shaped filaments. no comparable intranuclear structures are observed in spermatogonia, secondary spermatocytes or spermatids. the threads begin to form in young spermatocytes, grow throughout spermatocyte development, reach their maximum size in mature spermatocytes and disintegrate prior to meiotic metaphase i. the presence ... | 1988 | 2465201 |
| an abundant testis rna species shows sequence similarity to y chromosomal and other genomic sites in drosophila hydei. | a cdna clone bank was constructed from testis poly(a)+ rna of drosophila hydei and screened for clones which hybridize to y chromosomal dna sequences. the insert of clone cdht14 hybridizes to a family of repeated dna sequences with members distributed within the y chromosome and elsewhere in the genome. this type of sequence has earlier been described as the y-associated class of dna. southern blot analysis of dna from different wild-type strains of d. hydei suggests that members of the t14 fami ... | 1989 | 2468992 |
| the transcriptional control regions of the copia retrotransposon. | we have analysed the sequence elements that control expression of the copia retrotransposon. expression of copia fusion constructs containing dna sequence deletions and rearrangements was assayed by transient expression analysis. progressive deletion and linker substitution identifies two regions on either side of the major transcriptional start sites in the copia long terminal repeat these regions are both required for high level expression in a cultured drosophila melanogaster cell line but on ... | 1989 | 2472602 |
| molecular cloning of a drosophila potential z-dna forming sequence hybridizing in situ to a developmentally regulated subdivision of the polytene chromosomes. | we describe the selection of a group of plasmids with potential to form z-dna, from libraries of drosophila hydei nuclear dna using anti z-dna monoclonal (22) or polyclonal (10c) antibodies. the supercoiled closed circular forms of most of the selected recombinant plasmids from the 10c z-dna library show affinity to the polyclonal 10c antibody as indicated by dna binding assays. one of these plasmids, pf17, was selected for further study. the insert in this plasmid adopts the z conformation at b ... | 1989 | 2473437 |
| a group of non-y encoded drosophila hydei primary spermatocyte nuclear glycoproteins exhibits epitopes depending on formation of y chromosomal giant lampbrush loops. | in wild-type drosophila hydei (genotype x/y) four different primary spermatocyte nuclear glycoproteins, classified as non-y encoded because of their occurrence in x/o genotypes, were demonstrated to possess a few epitopes that depended on formation of the y chromosomal giant lampbrush loops threads (th; mr 55,000 proteins) or pseudonucleolus (ps; mr 38,000, 58,000 and 98,000 proteins). the epitopes reacted with lectins and/or antibodies in vitro lectin-/immunoreplica of primary spermatocyte tota ... | 1989 | 2476280 |
| natural selection and ribosomal dna in drosophila. | natural populations of drosophila mercatorum are variable for the number of x-linked 28s ribosomal genes bearing a 5-kilobase insert. a separate polymorphic x-linked gene controls whether 28s repeats bearing the insert are preferentially underreplicated during the formation of polytene tissue. female flies having at least a third of their 28s genes bearing the insert and lacking the ability to preferentially underreplicate inserted repeats display the abnormal abdomen syndrome. the syndrome is c ... | 1989 | 2556326 |
| characterization of the long terminal repeats of micropia elements microdissected from the y-chromosomal lampbrush loops "threads" of drosophila hydei. | four micropia elements from drosophila melanogaster and d. hydei have been analysed by sequencing. two elements, from d. hydei, micropia-dhmif8 and -dhmif2, were recovered by cloning microdissected y-chromosomal lampbrush loops "threads". this method allows isolation of repetitive sequences from defined chromosomal positions, but recovery of large and overlapping inserts is difficult. in case of the y-chromosomal micropia elements it was not possible to define the endpoints of their long termina ... | 1989 | 2585498 |
| evolution of y chromosomal lampbrush loop dna sequences of drosophila. | the evolutionary conservation of y chromosomal dna sequences of drosophila hydei in different species of the genus drosophila was studied by in situ hybridization and on genomic dna blots of restriction enzyme digested dna. we demonstrated that y specific dna sequences, which form major parts of lampbrush loops related to the male fertility genes, are only retained in a few closely related species during evolution. other y chromosomal dna sequences, also present in lampbrush loops but with homol ... | 2010 | 2881740 |
| heat shock locus 93d of drosophila melanogaster: a spliced rna most strongly conserved in the intron sequence. | the drosophila melanogaster heat shock locus at 93d encodes at least three overlapping transcripts, 10-12 kilobases (kb), 1.9 kb, and 1.2 kb. the abundance of the three transcripts is significantly increased during heat shock; however, all are also found in non-heat-shocked cells. the 1.2-kb transcript is found in the cytoplasm. sequence analysis of a 1.1-kb cdna clone representing sequences within the 1.2-kb transcript and comparison to genomic sequences indicate that it is spliced; 700 base pa ... | 1986 | 3081901 |
| a drosophila heat shock locus with a rapidly diverging sequence but a conserved structure. | cytological studies have shown that the heat shock loci 93d of drosophila melanogaster and 2-48b of drosophila hydei have several characteristics which suggest that they are homologous loci, yet sequence homology is barely detectable by cross-hybridization. using cloned dna sequences we have compared the two loci. both loci produce transcripts of similar size and number. we have characterized the three predominant transcripts. in each species all three transcripts start at or about the same plac ... | 1986 | 3097014 |
| heat shock loci 93d of drosophila melanogaster and 48b of drosophila hydei exhibit a common structural and transcriptional pattern. | a comparison of gene structure, sequence, and transcription pattern of heat shock loci 93d of drosophila melanogaster and 48b of drosophila hydei has been performed. both heat shock loci consist of an unique region that is flanked by an internally repetitive element. different members of these elements are highly conserved, repeat unit length, however, and primary sequence diverged totally. whereas the overall gene structure in both species is substantially related, sequence conservation is only ... | 1987 | 3106930 |
| structure and function of y chromosomal dna. i. sequence organization and localization of four families of repetitive dna on the y chromosome of drosophila hydei. | the sequence organization of four different families of y chromosomal repetitive dna is characterized at three levels of spatial extension along the y chromosome of drosophila hydei. at the lowest level of resolution, dna blot analysis of y chromosomal fragments of different lengths and in situ hybridization experiments on metaphase chromosomes demonstrate the clustering of each particular sequence family within one defined region of the chromosome. at a higher level of resolution, family specif ... | 1988 | 3349874 |
| z-dna in transcriptionally active chromosomes. | due to the striking correlation between the distribution of transcriptionally active subdivisions of the polytene chromosomes and z-dna, we have addressed the question of whether the z-dna configuration exists in native, transcriptionally active chromosomes of drosophila hydei prepared without interference by procedures known to induce the b to z conformation. our experiments indicate that z-dna forms are present in a specific set of sites on the native chromosomes. they occur on interbands and ... | 1987 | 3470742 |
| differential riboflavin deposition in white and variegated white mutants of drosophila hydei. | riboflavin deposition in organs of drosophila hydei was studied by means of a growth test using a riboflavin-deficient strain of the fungus aspergillus nidulans. in wild-type animals, riboflavin is deposited in malpighian tubules (mt) and testes but not in adult eyes. certain white (w) mutants do not contain riboflavin, whereas intermediately colored w mutants contain minor amounts of the substance. riboflavin-containing mt cells contain special globules that can be fixed and stained with the re ... | 1987 | 3502968 |
| differential chromosomal distribution of ribonucleoprotein antigens in nuclei of drosophila spermatocytes. | the ribonucleoprotein (rnp) composition of the active y chromosomal structures in spermatocyte nuclei of drosophila hydei has been investigated using the anti-rnp antibodies dm 28k2 and pp60 as a probe. antibody dm 28k2 was raised against an rnp protein of cytoplasmic rnp particles in d. melanogaster cells, while antibody pp60 was raised against a pre-messenger rnp fraction from oocytes of xenopus laevis. both antibodies detect nuclear rnp (nrnp) antigens of d. hydei. this is shown by cscl densi ... | 1986 | 3536959 |
| histones and histone acetylation during the embryonic development of drosophila hydei. | histones and histone acetylation have been investigated during three stages of drosophila hydei embryogenesis--early gastrula, late gastrula and organogenesis. no essential changes in the electrophoretic pattern of the histones have been revealed during the stages examined. however, we established an enhanced level of [14c]acetate incorporation at the time of extensive gene activation during gastrulation as well as some quantitative differences in the pattern of acetylation during gastrula and o ... | 1986 | 3757037 |
| changes in the pattern of histone h1 phosphorylation during drosophila hydei embryogenesis. | histone h1 phosphorylation was examined during embryonic development of drosophila hydei. a changing pattern of h1 phosphorylation upon separation on an acid-urea polyacrylamide gel was observed in the course of drosophila embryogenesis. it is considered to be related to the decrease of the mitotic activity of the cells as development proceeds. | 1986 | 3807901 |
| y chromosome function and spermatogenesis in drosophila hydei. | 2013 | 3887859 | |
| purification and molecular characterization of alcohol dehydrogenase from drosophila hydei: conservation in the biochemical features of the enzyme in several species of drosophila. | alcohol dehydrogenase (adh) has been purified from drosophila hydei. biochemical investigations show that the native enzyme is a dimer consisting of two identical subunits with molecular weight 27,000. the ph optimum values of pure enzyme preparations are 7.9 and 9.4. the pi values are 8.83 and 8.41. substrate specificities have been characterized. km(app) values are lowest for propan-2-ol and butan-2-ol and vmax(app) values are highest for these two substrates. the amino acid composition has be ... | 1985 | 3910022 |
| regulated expression of a drosophila melanogaster heat shock locus after stable integration in a drosophila hydei cell line. | dna-mediated cotransformation has been used to transfer a drosophila melanogaster heat shock locus into cultured drosophila hydei cells by use of the copia-based selectable vector pcv2gpt and of pmh10a, a cloned 87a7 heat shock locus encoding a mutant heat shock protein (hsp). transformed lines contain between 50 and 200 copies of both plasmids, each separately organized as a head-to-tail concatemer which is stably maintained in the transformed lines. exposure of the cotransformants to heat shoc ... | 1985 | 3939313 |
| isolation of y-chromosomal repetitive dna sequences of drosophila hydei via enrichment of chromosome-specific sequences by heterogeneous hybridization between female and male dna. | male or female dna of drosophila hydei was sheared by sonication, denatured, reannealed to different c0t-values and fractionated by hydroxyapatite. the highly repetitive, moderately repetitive and unique fractions of female dna were denatured again and coupled via diazotization or cyanogen bromide to macroporous sephacryl s-500. enrichment of y-chromosomal sequences was achieved by cycling each of the different fractions of male dna under optimized hybridization conditions over a column with a m ... | 1986 | 3944419 |
| locations of z-dna in polytene chromosomes. | in polytene chromosomes of drosophila hydei and d. melanogaster, z-dna was identified in varying distribution after different conditions for fixation were used. when salivary glands were fixed and squashed in 50% acetic acid alone, z-dna was found in the less dense dna regions, such as interbands, some puffs, and a few of the less dense bands. prefixation that combined ethanol and acetic acid exposure led to prominent immunofluorescent staining of the bands, generally but not strictly correlatin ... | 1985 | 3988806 |
| local protein accumulation during gene activation. i. quantitative measurements on dye binding capacity at subsequent stages of puff formation in drosophila hydei. | 1970 | 4104059 | |
| production and release of a locus-specific ribonucleoprotein product in polytene nuclei of drosophila hydei. | a specific, 0.1-0.3-microm large ribonucleoprotein complex consisting of a central core with stalklike extensions on top of which 280-320-a ribonucleoprotein particles are situated is found in an experimentally activated chromosome region, 2-48c, of the polytene chromosomes of drosophila hydei. alkaline hydrolysis, rnase digestion, and uranyl-edta-lead staining indicated the ribonucleoprotein character of the 280-320-a particles, whereas the central core seems to be devoid of rna. the characteri ... | 1973 | 4128322 |
| the replicative organization of dna in polytene chromosomes of drosophila hydei. | 1968 | 4181535 | |
| differentiation of transplanted larval salivary glands of drosophila hydei in adults of the same species. | 2014 | 4222967 | |
| time sequence in the expression of various developmental characters induced by ecdysterone in drosophila hydei. | 1970 | 4248971 | |
| metabolic changes during spermatogenesis and thoracic tissue maturation in drosophila hydei. | 1972 | 4402328 | |
| the influence of ouabain on the elimination of injected and orally applied dyes in drosophila hydei. | 1974 | 4421313 | |
| ecdysone-binding proteins in haemolymph and tissues of drosophila hydei larvae. | 1974 | 4424235 | |
| [regulation of ion concentration by drosophila hydei na+, k+, ca2+, and cl-]. | 2015 | 4425097 | |
| model approaches for the evaluation of electrical cell coupling in the salivary gland of the larva of drosophila hydei. the influence of lysolecithin on the electrical coupling. | 1974 | 4444018 | |
| [differential radiosensitivity of the germ cells in drosophila hydei males, lethal rates and the relevance of the species-specific genetic organization (author's transl)]. | 2015 | 4469156 | |
| structure and function in the genome of drosophila hydei. | 1974 | 4524779 | |
| local structural variations of the y chromosome of drosophila hydei and their correlation to genetic activity. | 1974 | 4524780 |