Publications

[morphological and taxonomical studies on anisakidae larvae found in marine fishes of china. ii. gulf of tong king].	a survey on anisakidae larvae in 29 species (134 specimens) of marine fishes in the gulf of tong king has been carried out. anisakidae larvae were detected in 15 out of 29 species. the detected specimens were identified as larvae of anisakis simplex, hysterothylacium and pseudoterranova. the parasitization rate of anisakis simplex larvae, the main pathogen of anisakiasis, in fishes was 30.6% (41/134), while the parasitization rates of hysterothylacium and pseudoterranova larvae were comparativel ...	1992	1394905
the importance of sculpin (myoxocephalus scorpius) as intermediate host and transmitter of the sealworm pseudoterranova decipiens.	a total of 186 sculpins (myoxocephalus scorpius) were caught at vega, nordland, norway, and examined for sealworm larvae (pseudoterranova decipiens). over 80% of the fish were infected. very high infections were found with a maximum of nearly 300 larvae in one sculpin. infections of this magnitude have so far not been reported from sculpins or any other fish species of this size. the importance of the sculpin in the life cycle and in the transmission of p. decipiens to seals or other fish specie ...	1992	1399251
organization of non-vertebrate globin genes.	the organization of non-vertebrate globin genes exhibits substantially more variability than the three-exon, two-intron structure of the vertebrate globin genes. (1) the structures of genes of the single-domain globin chains of the annelid lumbricus and the mollusc anadara, and the globin gene coding for the two-domain chains of the clam barbatia, are similar to the vertebrate plan. (2) genes for single-domain chains exist in bacteria and protozoa. although the globin gene is highly expressed in ...	1992	1478060
[anisakiasis in spain. report of 3 cases].	we reported here three cases of non-imported anisakiasis in spanish patients. eating raw sardines was the only common epidemiological feature. two of the cases presented clinically as a bowel obstruction, and underwent an emergency surgical procedure. in the intestinal biopsy a larva of anisakiasis simplex was found in the abdominal wall, surrounded by a eosinophilic granuloma and large edema. the third case presented as a peptic ulcer disease with longer evolution time, and a larva of pseudoter ...	1992	1576191
on the ecology and distribution of pseudoterranova decipiens c (nematoda: anisakidae) in an intermediate host, hippoglossoides platessoides, in northern norwegian waters.	the distribution of pseudoterranova decipiens c helps demonstrate the ecological basis of this genetically defined sibling species. in northern norwegian waters the major fish intermediate host is hippoglossoides platessoides. overall prevalence, mean intensity and intensity range in h. platessoides were 15%, 16.5 and 1-165, respectively. outside the range of its only known definitive host, the seal erignathus barbatus, the parasite was not found in the same intermediate host.	1992	1587684
gastrointestinal helminths of the cetaceans phocoena spinipinnis (burmeister, 1865) and cephalorhynchus eutropia (gray, 1846) from the southern coast of chile.	gastrointestinal helminths of the cetaceans cephalorhynchus eutropia and phocoena spinipinnis accidentally entangled in gillnets off the coast of queule, chile, were identified from 1989 to 1990. pseudoterranova sp., polymorphus (polymorphus) cetaceum and synthesium tursionis occurred in both cetaceans. additionally, anisakis sp. and braunina cordiformis were found in c. eutropia, and anisakis simplex was identified from p. spinipinnis. the species with the highest prevalence and mean intensity ...	1992	1602588
pseudoterranova decipiens larva infection mimicking a chronic peptic ulcer.		1992	1615964
functional relationship between sealworm (pseudoterranova decipiens, nematoda, ascaridoidea) burden and host size in atlantic cod (gadus morhua).	a model is proposed to describe the accumulation of sealworm parasites (pseudoterranova decipiens, nematoda, ascaridoidae) in atlantic cod (gadus morhua). the model predicts the total amount of food eaten by a fish to reach its size. in young fish growth is linear in time and average parasite burdens are predicted to increase with the cube of the average length of the fish divided by its growth rate. an analysis of worm burdens in cod caught by the commercial fishery in the firth of clyde (strat ...	1991	1682940
the helminth fauna from the common seal (phoca vitulina vitulina, linné, 1758) of the wadden sea in lower saxony. part 2: nematodes.	during the seal epidemic in 1988 and the beginning of 1989, 115 common seals found dead on the shores of the wadden sea of lower saxony were investigated for the presence of nematodes. the lungworm otostrongylus circumlitus railliet 1899 was found in 26.1% of the seals, the lungworm parafilaroides gymnurus railliet 1899 in 26.9% and the heartworm dipetalonema spirocauda leidy 1858 in 32.2% of the seals. in the digestive tract, two anisakid species were found, pseudoterranova decipiens mozgovoi 1 ...	1991	1789022
genetic evidence for three species within pseudoterranova decipiens (nematoda, ascaridida, ascaridoidea) in the north atlantic and norwegian and barents seas.	genetic variation of 1017 specimens of codworm, pseudoterranova decipiens, collected from fish and seals at 23 sampling locations in the north atlantic and norwegian and barents seas, was analysed on the basis of 16 enzyme loci. three reproductively isolated species, provisionally designated p. decipiens a, b and c, were detected, showing distinct alleles at the following loci: mdh-1, 6pgdh, np, pgm, est-2 (between species a and b); mdh-3, 6pgdh, np, sod-1, adk, pgm, est-2, mpi (between a and c) ...	1991	1869354
isolation and sequencing of a cdna for an unusual hemoglobin from the parasitic nematode pseudoterranova decipiens.	a cdna clone encoding a 333-amino acid hemoglobin was isolated from the nematode pseudoterranova decipiens. the protein contains an 18-amino acid hydrophobic signal sequence and has a calculated mass of 37.6 kda in the mature form. the predicted protein reveals an internal duplication of a 154-amino acid domain (51% identity). both domains have significant sequence homology to other primitive hemoglobins, in agreement with a duplication event. hydrophobicity plots reveal identical strongly hydro ...	1991	2062843
first record of pseudoterranova (nematoda: anisakidae) from the chilean dolphin, cephalorhynchus eutropia (cetacea: delphinidae).	larvae of third and fourth stages of pseudoterranova were isolated for the first time from cephalorhynchus eutropia in south coast of chile. morphological characteristics of hosts and parasites are given.	1989	2487513
in vitro effect of ivermectin on pseudoterranova decipiens survival.	third larval stages (l3) removed from fish fillets, fourth larval stages (l4) raised in in vitro culture, and adults of pseudoterranova decipiens, collected from grey seal (halichoerus grypus) stomachs, were exposed to the broad spectrum anthelmintic, ivermectin. l3 and l4 parasites were exposed, in vitro, to 500, 100, 50, 20, 5 and 1 micrograms/ml concentrations of the drug, in culture media. adult p. decipiens were exposed in vitro to a concentration of 500 micrograms/ml ivermectin, only. cont ...	1989	2723387
[anisakids and human anisakiasis. 1. bibliographic data].	currently available data about larvae of anisakidae (genera anisakis, pseudoterranova, hysterothylacium and contracaecum) from marine fishes are analysed. hazard of such parasites for fish-consumers are emphasized.	1988	3048180
[anisakids and human anisakiasis. 2. investigation of the anisakids of commercial fish in the district of paris].	1,173 fish belonging to 13 species were examined during 1986-1987, during a survey conducted among commercial marine fish in the paris area (france). anisakis simplex l3 were found in 10 different species, and most frequently in herring (clupea harengus) 82.55% of 682 fish, red fish (sebastes marinus) 86.11% of 36 fish, and hake (merluccius merluccius) 88.57% of 35 fish, and in high number (average 8.8, 18.4 and 31 l3 per fish). pseudoterranova decipiens l3 were recovered from 7 different specie ...	1988	3190121
nematode infestation of fillets from atlantic cod, gadus morhua, off eastern canada.	the prevalence and intensity of larval nematodes in fillets of atlantic cod, gadus morhua, were examined and compared with similar data from a previous survey conducted about 30 yr ago. anisakis simplex occurred more often in the nape of the fillet, whereas pseudoterranova decipiens was the predominant species in napeless fillets. the results suggest an increase in both the prevalence and intensity of p. decipiens in fillets of cod, especially those originating from the gulf of st. lawrence and ...	1988	3193325
[transition of occurrence of anisakiasis and its paratenic host fishes in japan, with pathogenesis of anisakiasis].	the number of anisakiasis according to a nation-wide statistical survey totaled 4682 cases until june 1987 in japan. of these 4296 cases are gastric anisakiasis (including 215 cases of gastric terranovasis), 375 intestinal anisakiasis and 11 extra-gastrointestinal anisakiasis. pseudoterranova larva does not invade the intestine, and worms are vomited in most cases. clinical diagnosis of intestinal anisakiasis is more difficult than that of gastric anisakiasis, and it also is hard to find the wor ...	1988	3292360
a simple technique for recovering larval ascaridoid nematodes from the flesh of marine fish.	this paper describes and evaluates the efficiency of a simple technique for recovering larval ascaridoid nematodes (anisakis simplex and pseudoterranova decipiens) from the flesh of marine fish. the technique involves mechanical disintegration of the flesh in a domestic food processor, followed by visual inspection of diluted portions of the resulting homogenate under short-wave ultraviolet light. the nematodes, which remain intact, fluorescence brightly and are easily detected, particularly if ...	1988	3397837
the value of external morphology in the identification of larval anisakid nematodes: a scanning electron microscope study.	we studied larval nematodes of four genera of the anisakidae using a scanning electron microscope (sem). the anterior and posterior extremities and cuticular structures of the 3rd-stage larvae (l3) of anisakis type i, pseudoterranova decipiens, contracaecum type b and hysterothylacium were examined. the 4th-stage larvae (l4) of anisakis type i, p. decipiens, recovered after infection into laboratory rats, and the l3 and l4 of anisakis type i larvae from human were also examined in the same way. ...	1986	3799008
[occurrence of parasites, bacteria, viruses and fungi in fish that are pathogenic to men and fish].	parasitological examinations comprised above 20,000 fish which were searched for parasitic nematoda of anisakidae. it was evidenced that herring were infected with anisakid larvae in 8%, cod and flatfish in 4% and the eelpouts in as many as in 52%. the species that prevails in these fish were: anisakis simplex, contracaecum osculatum c, hysterothylacium auctum and pseudoterranova decipiens, respectively. in direction of the bacteria pathogenic to man 765 fish were examined; in 38% there were fou ...	1995	7571631
sealworm (pseudoterranova decipiens) infection in common seals (phoca vitulina) and potential intermediate fish hosts from the outer oslofjord.	infection with pseudoterranova decipiens in 45 common seals (phoca vitulina) from the outer oslofjord was investigated. seals were collected before and during the phocine distemper virus epizootic in 1988 when the seal population in the area was reduced from about 350 to 100. in 1989, cod (gadus morhua) and other fish species were sampled for comparison with earlier investigations on prevalence and abundance of p. decipiens infection in fishes from the outer oslofjord. seventy-two per cent of co ...	1995	7601595
anisakidosis: report of 25 cases and review of the literature.	anisakidosis (previously known as anisakiasis) is a disease caused by the accidental ingestion of larval nematodes (anisakis and sometimes pseudoterranova) in raw fish. two groups of patients are studied: 5 clinical cases and 20 serological diagnoses. 55 french cases are already published. most of them complained of acute symptoms, which occurred within 12 h of eating the seafood meal--epigastric pain, occlusion, diffuse abdominal pain, and appendicitis. larvae were attached to the gastric mucos ...	1995	7621671
structural characterization of an ascaris myoglobin.	globin was purified from the body wall of adults of the parasitic nematode ascaris suum. internal peptide fragments were sequenced and cdnas encoding a polypeptide of 154 amino acids isolated by polymerase chain reaction. the polypeptide lacks a signal sequence, identifying it as a cytosolic myoglobin-like species. the native protein is a dimer. the predicted amino acid sequence shares several unusual substitutions with other nematode globins. like the abundant pseudocoelomic a. suum hemoglobin ...	1994	7982924
[the problem of anisakiasis].	presents a brief analysis of the basic findings concerning the pathogenesis, clinical picture, diagnosis, prevention, and epidemiology of anisakiasis in the world. suggests an opinion on a high prevalence of anisakiasis in some regions of russia, where it is erroneously diagnosed as some other gastrointestinal diseases. presents the author's data on the infestation of fish with anisakis simplex and pseudoterranova decipiens larvae in the okhotsk and bering seas. emphasizes the necessity of devel ...	1993	8028570
molecular genealogy of some nematode taxa as based on cytochrome c and globin amino acid sequences.	we have begun to reconstruct the ancient history of the nematode phylum based on cytochrome c and globin amino acid sequences. the data suggest that the nematode ancestor diverged from a line leading to mammals about 1 billion years ago and that the most recent common ancestor of the extant species caenorhabditis elegans, trichostrongylus colubriformis, nippostrongylus brasiliensis, ascaris suum, and pseudoterranova decipiens lived about 550 my ago. the rhabditids and strongylids emerged as one ...	1994	8075836
a human case of gastric anisakiasis by pseudoterranova decipiens larva.	a case of gastric anisakiasis due to the larva of pseudoterranova decipiens was confirmed by gastroendoscopic examination in april 23, 1991. the patient, residing in pusan, was a 42-year-old housewife, who complained of severe epigastric pain and recalled that the symptom suddenly attacked her about 6 hours after eating raw sebastes inermis. in the gastroendoscopic examination performed about 9 hours after the onset of the symptom, a long whitish nematode larva penetrating the gastric mucosa in ...	1994	8167109
larval nematodes infecting amphiporeia virginiana (amphipoda: pontoporeioidea) on sable island, nova scotia.	three species of larval nematodes were found infecting the nearshore subtidal amphipod amphiporeia virginiana collected from beaches on sable island, nova scotia. five and 2 sealworms, pseudoterranova decipiens s.l., were found in 2,020 and 2,044 amphipods, respectively, collected in 1991 and 1992, dissected, and examined microscopically. two sealworms, 1 paracuaria adunca and 1 ascarophis sp., were found in 5,157 amphipods collected in 1992 and enzymatically digested. infection with each specie ...	1993	8277392
novel gene structure and evolutionary context of caenorhabditis elegans globin.	animal and plant globin-encoding genes (glo) contain two introns in strictly conserved positions. plant glo genes possess an additional, centrally located intron. we have determined the cdna sequence and gene structure of a putative glo gene from the free-living nematode, caenorhabditis elegans. the gene encodes a one-domain globin with a single intron, corresponding to the central intron of plant glo genes. the two introns common to virtually all animal and plant glo genes are missing. comparis ...	1993	8325507
kinetics of ligand binding to pseudoterranova decipiens and ascaris suum hemoglobins and to leu-29-->tyr sperm whale myoglobin mutant.	the kinetics of binding of o2, co, and no to the octameric, two-domain hemoglobins of the parasitic nematodes pseudoterranova decipiens and ascaris suum were determined on nanosecond and picosecond time scales using flash photolysis. the two nematode hemoglobins have very similar kinetic properties. on the picosecond time scale, they exhibit an unusual behavior in showing a geminate reaction with oxygen that is biphasic and dependent on the flash intensity. the geminate reaction with no is also ...	1993	8349589
stress-induced failure of osmoregulation in the parasitic nematode pseudoterranova decipiens: indirect evidence for hormonal regulation.	when third-stage larvae of pseudoterranova decipiens maintained at 5 degrees c are placed in either 40% artificial sea water (asw, iso-osmotic) or 15% asw (hypo-osmotic) and weighed once at 0 h and again at 24 h, they neither lose nor gain weight, and the osmotic pressure (op) of their pseudocoelomic fluid (pcf) remains unchanged. in contrast, when worms are weighed six additional times during the 24 h interval, those maintained in isoosmotic conditions lose weight, while those maintained in hyp ...	1993	8371086
osmoregulation in the parasitic nematode pseudoterranova decipiens.	when subjected to hyper- or hypo-osmotic stress at 5 degrees c for 24 h, third-stage larvae of the parasitic nematode pseudoterranova decipiens do not exhibit changes in mass or in the osmotic pressure of the pseudocoelomic fluid. immersion in solutions containing 3h2o demonstrates that exchange with the water in the pseudocoelomic fluid is substantially complete within 24 h. sacs composed of cylinders of body wall without the intestine and pseudocoelomic fluid do not gain weight when immersed f ...	1993	8440971
water compartments and osmoregulation in the parasitic nematode pseudoterranova decipiens.	a study of the time course of penetration of 3h2o into whole worms suggests that worms immersed in a hypo-osmotic environment (15% artificial sea water) reach full exchange equilibrium more slowly than worms in an iso-osmotic environment (40% artificial sea water). the apparent water content, determined by dry mass, matches that determined by 3h2o exchange when worms are immersed for 24 h in 40% artificial sea water (asw), but the water content measured by 3h2o exchange is lower when worms are k ...	1993	8440972
three sibling species within contracaecum osculatum (nematoda, ascaridida, ascaridoidea) from the atlantic arctic-boreal region: reproductive isolation and host preferences.	genetic variation within and between population samples from 22 locations of the atlantic arctic-boreal region, including 1657 specimens morphologically assigned to contracaecum osculatum, was electrophoretically analysed at 17 loci. highly significant deviations from the hardy-weinberg equilibrium were found at various loci in several samples, owing to the existence of three distinct gene pools within c. osculatum (sensu lato) from the study area. these gene pools correspond to three biological ...	1993	8468125
[gastric anisakiasis cases in cheju-do, korea].	human anisakiasis may occur after ingestion of raw marine fish infected with nematode larvae of anisakidae. anisakiasis caused by the migration of the larva into the wall of stomach, small intestine and other portion has been reported in korea. this prospective study was made of all cases referred to parasitological laboratory in cheju-do between june 1989 and june 1992. gastric anisakiasis was confirmed if larvae invading the gastric wall were observed by gastrofiberscopy. one hundred and seven ...	1995	8528624
recovery of pseudoterranova decipiens (anisakidae) larvae from codfish of the antarctic ocean.	anisakid larvae were found from the liver and stomach of the codfish (notothenia neglecta) caught from the antarctic ocean, south pole, where the polar research center of the korea ocean research and development institute is operating. through observations by scanning electron (sem) and light microscopy (lm), they were identified as the third stage larvae of pseudoterranova decipiens, a potential agent of human codworm anisakiasis. they measured 25.0-28.5 mm in length, and 0.6-0.8 mm in width. t ...	1995	8528631
[parasitologic significance of the alteration of the causative anisakidae worm and of the pseudoterranova decipiens female immature adult worm, casting off the cuticles, and excreted from human in kanazawa city].	we have been studying anisakidae larvae, their intermediate hosts and their final hosts in the northern japan sea area. these larvae cause anisakidosis. according to the investigation, the recent burst of pseudoterranovosis in this area can be attributed to the increased presence of sea lions, which proliferate in the arctic region, then migrate to the northern japan sea and eat the intermediate host fish. in a stomach of a male sea lion that was captured in february 1995, we found more than 4,5 ...	1995	8543275
micro-evolutionary implications of allozymic and morphometric variations in sealworms pseudoterranova sp. (ascaridoidea: anisakidae) among sympatric hosts from the southeastern pacific ocean.	we found significant morphometric and electrophoretic differences between sealworm larvae collected from four sympatric fish host species off the central coast of chile. the south american sea lion, otaria byronia, is a suitable host and most likely the only definitive host species in the study area. morphological patterns of caudal papillae in adult males collected from sea lions and electrophoretic evidence from larvae and adults substantiate our conclusion that they belong to just one, new sp ...	1995	8557462
gastric anisakidosis due to pseudoterranova decipiens larva.		1996	8667934
the infection status of anisakid larvae in marine fish and cephalopods from the bohai sea, china and their taxonomical consideration.	the infection status of anisakid larvae was examined in 290 marine fish of 25 species and in 108 cephalopods of 3 species purchased in bayuquan region, yingko city nearby the coast of the bohai sea from may to august 1992. a total of 7,327 larvae were collected from 156 fish of 19 species and 8 squids of one species. the 3rd-stage larvae of anisakis simplex were collected from 121 fish (63.4%) of 15 species (n = 191) and from 8 squids (14.8%) of one species (n = 54), and they were total, 5,992 ( ...	1997	9100435
settling rate and dispersal of sealworm eggs (nematoda) determined using a revised protocol for myxozoan spores.	settling rates and densities of small bodies can be determined by recording settling rates in fluids of different densities, but similar viscosities, and applying stoke's law. however, at low reynolds numbers, drag effects reduce the object's settling rate and must be accounted for in sedimentation experiments. using a revised protocol for spores of the myxozoan henneguya doori that accounted for significant drag effects exerted by the experimental apparatus, we improve upon a previously describ ...	1997	9105296
experimental infection/transmission of sculpins (myoxocephalus scorpius) and cod (gadus morhua) by sealworm (pseudoterranova decipiens) larvae.	five serial transmission experiments with sealworm larvae were carried out. pseudoterranova decipiens third-stage larvae (l3) were transferred via a stomach tube from sculpin to cod, from cod to sculpin, from sculpin to sculpin, and from cod to cod. after a minimum of 37 days the recipient fish were killed and the numbers of surviving p. decipiens larvae were recorded. p. decipiens transferred to sculpins showed the greatest rate of survival.	1997	9134562
fecundity of sealworm (pseudoterranova decipiens) infecting grey seals (halichoerus grypus) in the gulf of st. lawrence, canada: lack of density-dependent effects.	uterine egg counts were determined by means of an electronic particle counter in individual female sealworm, pseudoterranova decipiens (nematoda: ascaridoidea: anisakidae) collected from grey seals (halichoerus grypus) from anticosti island, northern gulf of st. lawrence, in august and september 1992. mean number of eggs per female was 54,916.9 (+/- 51,866.4), with a maximum of 300,000. egg counts were strongly correlated with worm length, and less so with host sex and age, sealworm sex ratio, a ...	1997	9421731
helminth parasites of epinephelus morio (pisces: serranidae) of the yucatan peninsula, southeastern mexico.	the present paper comprises a systematic survey of helminths from 202 red groupers, epinephelus morio (valenciennes) (pisces: serranidae), the most important commercial marine fish in the region, collected from ten localities off the yucatan peninsula in the gulf of mexico during 1994-1996; two more helminth species were recorded from e. morio earlier. thirty species of helminths were found: monogenea 1, cestoda 3, trematoda 17, nematoda 8, acanthocephala 1. of them, 15 species were adults, wher ...	1997	9437838
human case of gastric infection by a fourth larval stage of pseudoterranova decipiens (nematoda, anisakidae).	only three cases of human infection by anisakid nematodes have been reported in chile since 1976. in the present case, an anisakid worm, identified as a fourth-stage pseudoterranova decipiens larva, was removed with a gastroendoscopic biopsy clipper from the stomach of a 45 year-old man from southern chile. the patient, who presented acute epigastric pain and a continuous sensation of having an empty stomach, reported having eaten smoked fish. the worm was fixed in 70% ethanol and cleaned in lac ...	1997	9497566
parasites of fish and risks to public health.	a multitude of parasites have been reported in fish, but only a few species are capable of infecting humans. the most important of the helminths acquired by humans from fish are the anisakid nematodes (particularly anisakis simplex and pseudoterranova decipiens), cestodes of the genus diphyllobothrium and digenetic trematodes of the families heterophyidae, opisthorchiidae and nanophyetidae. seafood-associated infections by acanthocephalans are rarely reported in humans. all of the helminths ment ...	1997	9501379
photo quiz ii. luminal anisakidosis due to pseudoterranova decipiens.		1998	9597231
parasitation with pseudoterranova decipiens (nematoda) influences the survival rate of the european smelt osmerus eperlanus retained by a screen wall of a nuclear power plant.	a total of 354 adult european smelts osmerus eperlanus (l.) were tested for their ability to survive the screen system of the cooling water inflow of a power plant. with increasing number of musculature parasitic third-stage larvae of pseudoterranova decipiens, the survival rate of o. eperlanus decreased while the total number of externally visible injuries as well as the number of seriously injured specimens increased. the results indicate that even a single specimen of p. decipiens influences ...	1998	9676248
occurrence of anisakis physeteris baylis, 1923 and pseudoterranova sp. (nematoda) in pygmy sperm whale kogia breviceps (de blainvillei, 1838) (physeteridae) in northeastern coast of brazil.		1998	9698890
parasites and related pathological lesions in the gastrointestinal tract of a seal (phoka vitulina linnaeus).	an immature seal, about one-year-old, died a natural death. at autopsy, a large number of contracaecum osculatum and pseudoterranova decipiens, as well as some of anisakis simplex were found in the stomach together with numerous corynosoma strumosum in the small intestine and colon. histopathological examination revealed epithelial desquamation, congestion and hemorrhage in the mucosa. there were cellular infiltration and fibroplasia around the nematodes, collapsed nematodes and molted nematode ...	1998	9795905
determinants of ascaris hemoglobin octamer formation.	the oxygen-avid, homooctameric hemoglobin of ascaris (ah) has an unusual structure. each polypeptide consists of two tandem globin folds followed by a highly charged cooh-terminal tail that contains four direct repeats of his-lys-glu-glu (hkee). deletion analysis of the ah tail determined that at least two of the four hkee repeats are required for efficient octamer formation. surprisingly, the first four residues of the tail (glu-his-his-glu) alone were moderately effective in promoting multimer ...	1998	9830004
a case of gastric pseudoterranoviasis in a 43-year-old man in korea.	a case of pseudoterranova decipiens infection was found in a 43-year-old man by gastroendoscopic examination on august 20, 1996. on august 6, 1996, he visited a local clinic, complaining of epigastric pain two days after eating raw marine fishes. although the symptoms were relieved soon, endoscopic examination was done for differential diagnosis. a white, live nematode larva was removed from the fundus of the stomach. the larva was 38.3 x 1.0 mm in size and had a cecum reaching to the mid-level ...	1999	10188383
anisakidae larval infection in fish fillets sold in belgium.	fish fillets (n = 1760) sold on the belgium market were examined for anisakidae larval infection. seven sea fish species were examined by means of the candling technique over a period of 4 months, from november 1996 to february 1997. third-stage larvae of anisakis simplex and pseudoterranova decipiens were identified. a. simplex was the most abundant species with a prevalence of 84.5%, and was found in 192 of the examined fillets (10.9%). pollock (pollachius pollachius) was the most heavily infe ...	1999	10321017
ecology of pseudoterranova decipiens (krabbe, 1878) (nematoda : anisakidae) from antarctic waters.	the present study provides further data on the occurrence of pseudoterranova decipiens in fish from two different sampling sites in the antarctic. a total of 690 fish belonging to 33 species from the eastern weddell sea and 322 fish belonging to 12 species from the south shetland islands were examined. altogether, 23 fish species were found to be infested and 11 new host records could be established. p. decipiens occurred at a water depth of between 80 and 820 m. chaenocephalus aceratus and noto ...	1999	10431726
pseudoterranova decipiens species a and b (nematoda, ascaridoidea): nomenclatural designation, morphological diagnostic characters and genetic markers.	five genetically distinct and reproductively isolated species have been detected previously within the morphospecies pseudoterranova decipiens from the arctic-boreal, boreal and antarctic. morphological analysis was carried out on male specimens identified by genetic (allozyme) markers, allowing the detection of significant differences at a number of characters between two members of the p. decipiens complex, namely p. decipiens a and b. on the basis of such differences, the nomenclatural design ...	2000	10768762
natural transfer of helminths of marine origin to freshwater fishes with observations on the development of diphyllobothrium alascense.	infective stages of helminths of 5 species that occur as adults in marine mammals were found in burbot, lota lota (l.) (gadidae), from the lower kuskokwim river (southwestern alaska): diphyllobothrium alascense rausch et williamson, 1958; pyramicocephalus phocarum (fabricius, 1780); corynosoma strumosum (rudolphi, 1801); corynosoma semerme (forsell, 1904); and pseudoterranova decipiens (krabbe, 1878). some larval stages were obtained also from smelt, osmerus mordax dentex steindachner, an anadro ...	2000	10780552
'cebiche'--a potential source of human anisakiasis in mexico?	five fish species used for preparation of a popular dish (cebiche) made with raw fish flesh in mexico were obtained from five localities of the coast of yucatan. lutjanus synagris, gerres cinereus, sphyraena barracuda, epinephelus morio and haemulon plumieriwere examined for the presence of larvae of anisakid nematodes, causative agents of human anisakiasis. the nematode pseudoterranova sp. was found in e. morio and s. barracuda with a total prevalence of 83% and 6.5 +/- 6.2 worms per fish for e ...	2000	10881286
plasma and bile antibodies of the teleost trematomus bernacchii specific for the nematode pseudoterranova decipiens.	we investigated the occurrence of antibodies against protein antigens of the nematode parasite pseudoterranova decipiens in the plasma and bile of the antarctic teleost trematomus bernacchii. three different p. decipiens protein solutions were prepared: excreted/secreted proteins from live larvae (esp); surface-associated proteins obtained by mild extraction of larval bodies (sap); and cuticular soluble proteins recovered by extraction in strong reducing conditions (csp). using different immunoa ...	2000	10907137
anisakid larvae in the musculature of the argentinean hake, merluccius hubbsi.	we report the infection levels of third-stage anisakid larva in the muscle of the argentinean hake, merluccius hubbsi, in relation to fish size and location in the musculature. the musculature of 42 hake was separated into hypaxial (ventral) and epiaxial (dorsal) parts and surveyed for nematode larvae. two anisakid species were detected: anisakis sp. (prevalence, 52.4%; mean +/- sd abundance, 1.2 +/- 1.7) and pseudoterranova sp. (prevalence, 9.5%; mean +/- sd abundance, 0.2 +/- 0.7). since the f ...	2000	10945594
nematodes of elasmobranch fishes from the southern coast of brazil.	new records for nematode species recovered from elasmobranch fishes in brazil are established and new systematical arrangements proposed. parascarophis sphyrnae campana-rouget, 1955 from the spiral valve of sphyrna zygaena is referred for the first time in south america as a new host record. procamallanus (s.) pereirai annereaux, 1946, from the spiral valve of raja castelnaui is reported parasitizing an elasmobranch host. nematode larvae of the genera anisakis, contracaecum, pseudoterranova and ...	2001	11285478
a human case of gastric infection by pseudoterranova decipiens larva.	we report a case of gastric pseudoterranoviasis proven by gastrofiberscopy on dec. 13, 1994. the 34-year-old male patient, residing in chungju-shi, was admitted to konkuk university hospital complaining of prickling epigastric pain. the symptoms suddenly attacked him two days after eating raw marine fish at chonan-shi. by the gastrofiberscopic examination, a long white-yellowish nematode was found from the fundus region of stomach. the worm was 34.50 x 0.84 mm in size, and was identified as a 3r ...	2001	11441507
[helminthofauna of opisthonema liberate and harengula thrissina (osteichthyes: clupeidae) from chamela bay, jalisco, mexico].	we collected helminths from the "sardines" harengula thrissina (n = 61) and opisthonema libertate (n = 43), from chamela bay, jalisco state, mexico (12 and six species, respectively). the nematode pseudoterranova sp., reached the highest values of prevalence and mean abundance in o. libertate (11.6% and 0.13 worms per host, respectively), while in h. thrissina the digenean parahemiurus merus reached a prevalence of 49.1% and a mean abundance of 1.40 worms per host. low similarity values (qualita ...	2000	11487924
human infection by pseudoterranova decipiens (nematoda, anisakidae) in chile: report of seven cases.	from 1997 to 1999, we identified seven human cases of infection by fourth stage larvae of pseudoterranova decipiens in chile. all identified larvae were coughed up by the patients. subjects were 10-55 years old; five were female. some patients complained of coughing, expectoration, pharyngeal pain, nausea or anal and nasal pruritus. larvae of three patients were coughed up from 36 h to 7 days after having eaten raw (cebiche or sushi) or lightly fried fish. p. decipiens has a marine life cycle. i ...	2001	11500763
uterine adenocarcinoma with generalised metastasis in a bottlenose dolphin tursiops truncatus from northern patagonia, argentina.	an endometrial adenocarcinoma with areas of squamous differentiation and generalised metastasis was observed in a bottlenose dolphin tursiops truncatus stranded in northern patagonia in july 1997. this is the second report of a uterine adenocarcinoma in a free-living cetacean and the first in a delphinidae. this neoplasm likely compromised reproduction for several years. in addition, the dolphin presented tattoo-like skin lesions and its digestive tract was infested by anisakis simplex, pseudote ...	2002	12005238
allozyme and pcr-rflp markers in anisakid nematodes, aethiological agents of human anisakidosis.	in the framework of the researches granted by murst cofin97, studies on anisakid nematodes, aethiological agents of human anisakidosis, were carried out. the project was aimed to implement the knowledge on the systematics, genetics, ecology and epizootiology of species of the genera anisakis and pseudoterranova by applying genetic markers obtained from multilocus allozyme electrophoresis and from pcr-based techniques. the results obtained by allozyme studies allowed to extend the geographic dist ...	2001	12078474
infection with a black pseudoterranova decipiens.		2002	12085044
sscp-based identification of members within the pseudoterranova decipiens complex (nematoda: ascaridoidea: anisakidae) using genetic markers in the internal transcribed spacers of ribosomal dna.	the anisakid nematodes morphologically corresponding with pseudoterranova decipiens sensu lato (s.l.) (krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as pseudoterranova krabbei, p. decipiens (s.s.), p. bulbosa, p. azarasi and p. cattani by multilocus enzyme electrophoresis, were characterized using a dna approach. also a population of p. decipiens (s.l.) from chaenocephalus aceratus, the blackfin icefish, from antarctica and another from osmer ...	2002	12118717
the trouble with sealworms (pseudoterranova decipiens species complex, nematoda): a review.	sealworms or codworms, larvae of ascaridoid nematodes belonging to the pseudoterranova decipiens species complex, infect the flesh of numerous species of marine and euryhaline fish, and have proven a chronic and costly cosmetic problem for seafood processors. moreover, the parasite may cause abdominal discomfort in humans when consumed in raw, undercooked or lightly marinated fish. in this review, the phylogeny, life cycle and distributions of sealworms are discussed along with biotic and abioti ...	2002	12396224
population dynamics of sealworm, pseudoterranova decipienssensu lato, in sculpins, myoxocephalus scorpius, from two areas in norway between 1990 and 1996.	the abundance of sealworms (pseudoterranova decipienss.l.) in sculpins (myoxocephalus scorpius) was studied at torbjørnskjaer, southeast norway, and vega, northern norway, in samples from 1990 to 1996. the harbour seal ( phoca vitulina) population at torbjørnskjaer declined by 70% during the phocine distemper virus epizootic in 1988, and has increased during the 1990s. the abundance of sealworms in sculpins was relatively stable from 1992 to 1996 and any effect of the reduction in the number of ...	2003	12632153
human intestinal anisakiosis due to consumption of raw salmon.	anisakiosis is a parasitic infection that follows consumption of raw or insufficiently pickled, salted, smoked, or cooked wild marine fish infected with anisakis sp. larvae. we report a case of intestinal anisakiosis in a 50-year-old man from quebec who presented with abdominal pain and peripheral eosinophilia after eating raw wild-caught salmon from the pacific ocean off canada. abdominal ct scan showed bowel distension proximal to a segmental jejunal wall thickening, which was resected. the je ...	2003	12883252
[new case of human infection by a pseudoterranova decipiens larva (nematode, anisakidae) in peru].	the purpose of this report is to divulge a new case of human anisakidosis in peru, caused by a larva of the pseudoterranova decipiens nematode found in a 17 year old female patient from lima, who had ingested "cebiche" in a restaurant in the city of ica, in the central coast of peru. approximately 4 hours after having ingested the food, the patient reported a feeling of uneasiness, with a nauseous sensation in the epigastric region, which intensified 5 hours later when she vomited. in the vomit ...	2003	14532923
ascaridoid nematodes and associated lesions in stomachs of subadult male northern fur seals (callorhinus ursinus) on st. paul island, alaska: (1987-1999).	stomachs from subadult male northern fur seals (sams) (callorhinus ursinus) (n = 5,950) from st. paul island, alaska, were examined from 1987 to 1999 for lesions and parasites. on gross examination of these stomachs, parasite nodules were evident in 92% of the stomachs and active and healing ulcers were found in 14% and 10%, respectively. prevalence of nematodes from 1987 to 1997 was 88% for 5,700 sams for which numbers of parasites were estimated but not identified to the genus level. all nemat ...	2003	14535542
[gastric anisakidosis: personal experience].	anisakidosis is a parasitic disease of the human gastrointestinal tract caused by ingestion of marine nematode larvae such as anisakis simplex or, rarely, pseudoterranova, present in raw or undercooked fish. frequent sites of involvement by anisakis are the stomach, small intestine, rarely the colon, or the peritoneum, liver, pancreas, lung and tonsils, anisakidosis is a self-limiting disease; the symptoms arise 12-24 hours after ingesting raw fish and include nausea, diarrhoea, and severe abdom ...	2004	15152528
compounds of parasitic roundworm absorbing in the visible region: target molecules for detection of roundworm in atlantic cod.	the objective of this study was to contribute to the development of technology that will be able to replace manual operations in processing of fish fillets. removal of parasites, black lining, remnants of skin, and bloodstains are costly and time-consuming operations to the fish processing industry. the presence of parasites in fish products tends to spoil consumers' appetites. recent reports questioning the safety of eating cod infected with parasites might lower consumer acceptance of seafood. ...	2004	15270514
anisakiasis - is the sushi bar guilty?	since 1972, japan and some other countries such as the usa have seen a sudden increase in cases of anisakiasis (infection with anisakis sp.) and codworm an isakiasis (infection with the codworm, pseudoterranova [= phocanema] decipiens). larval stages of these nematodes occur in the muscles of a variety of fish and squid, and ingestion of raw and undercooked fish or squid is the prime route of human infection. thus the japanese style of eating sashimi (sliced raw fish fillet) and the increasing p ...	1987	15462905
immunoglobulin responses of northern elephant and pacific harbor seals naturally infected with otostrongylus circumlitus.	immunoglobulin (ig) binding patterns of pacific harbor seals (phs, phoca vitulina richardsi) and northern elephant seals (nes, mirounga angustirostris) to tissues of adult otostrongylus circumlitus were examined by immunoblotting to investigate the role of age in the unusual response of juvenile nes to infection with o. circumlitus. serum was taken from nes between march 1997 and march 2001 and from phs between may 1996 and august 1999. the serum of seals infected with o. circumlitus contained a ...	2004	15465714
[anisakidosis a marine parasitic zoonosis: unknown or emerging in peru?].	the purpose of this review is to show the experimental studies carried out on the biological cycle, culture, pathogenicity of the anisakidae nematode larvae and to disseminate the information regarding current epidemy and the probable emergence of anisakidosis in peru, and in addition, to propose measures of prevention and control, as well as the perspective and need for investigation. the studies of experimental pathogenicity in cats, dos, and hamsters are incomplete. eight cases of acute human ...	2004	15614302
nematode parasites of the digestive tract in neotropic cormorant chicks (phalacrocorax brasilianus) from the river cruces ramsar site in southern chile.	the neotropic cormorant (phalacrocorax brasilianus) breeding colony of the rio cruces nature sanctuary and ramsar site was surveyed for nematode parasites in southern chile. moribund chicks (n=128) of different ages were inspected for anchored nematodes using complete guts. we tested if chicks harbored the same parasites as the adults feeding them. besides the known contracaecum rudolphii, third or fourth stage larvae of pseudoterranova sp. and anisakis (type i and ii) were obtained in this wate ...	2005	15986255
[pseudoterranova decipiens (nematoda, anisakidae) larvae reported from humans in iceland after consumption of insufficiently cooked fish].	recently, two human cases of pseudoterranova decipiens nematode larvae were confirmed in iceland. in each case a larva was found in the throat five and six days after the consumption of insufficiently cooked filets of the common catfish anarhicas lupus that were bought fresh in a fish store. in both cases the larva had already developed from 3rd to the 4th stage during the time of infection. in the former case a young male woke up in the morning as he noticed a larva crawling around in his throa ...	2006	16400194
prevalence of anisakin nematodes in fish from southern baltic sea.	nematodes from the superfamily ascaridoidea (families anisakidae and raphidascarididae) are worldwide distributed parasites. their live cycles include many species of water invertebrates and teleostean fish as intermediate hosts, and fish, sea mammals or fish-eating birds being definitive hosts. humans can be infected with some of these parasites after consumption of raw or wrongly processed fish. the parasitological investigations of fish (herring, cod and flatfish) from southern baltic (ices 2 ...	2005	16457379
molecular phylogenetics and diagnosis of anisakis, pseudoterranova, and contracaecum from northern pacific marine mammals.	individual specimens of anisakis, pseudoterranova, and contracaecum collected from marine mammals inhabiting northern pacific waters were used for comparative diagnostic and molecular phylogenetic analyses. forty-eight new sequences were obtained for this study of 14 anisakis taxa, 8 pseudoterranova taxa, 4 contracaecum taxa, and 4 outgroup species. partial 28s (lsu) and complete internal transcribed spacer (its-1, 5.8s, its-2) ribosomal dna was amplified by the polymerase chain reaction and seq ...	2005	16539026
anisakid nematodes from stranded pygmy sperm whales, kogia breviceps (kogiidae), in three localities of the yucatan peninsula, mexico.	the present paper reports the presence of 3 adult and juvenile anisakid nematode species: anisakis simplex, a. brevispiculata, and pseudoterranova ceticola, which were recovered from the digestive tract of stranded pygmy sperm whales (kogia breviceps) from 3 localities along the coast of the yucatan peninsula. the presence of these anisakid adult nematodes suggests that larval stages may occur in cephalopods or fishes used for human consumption, which represents a potential danger to public heal ...	2006	17152967
[anisakiasis in a patient with a small hiatal hernia. report of one case].	we describe the presence of anisakiasis in a patient who had a small hiatal hernia. a 60 year-old women presented general malaise, burning pain, flatulence, persistent nausea and abdominal distension during five days before consulting. she referred that she ate a dish of marinated raw fish with lemon and pepper ("cebiche") and after a few hours the symptoms began. an esophagogastroscopy showed a white colour larva of approximately three cm with the cephalic end partially adhered and localized in ...	2007	17277874
acute gastric anisakiasis: an italian experience.	anisakidosis is a parasitic disease of the human gastrointestinal tract caused by ingestion of larvae of marine nematodes such as anisakis spp. or, rarely, pseudoterranova spp., present in raw or undercooked fish. we report the first series of gastric anisakis infection (anisakiasis) from a single centre in italy. in our department, we observed 3 cases, all in women who were urgently hospitalized following intense epigastric pain and vomiting, developed after the ingestion of raw fish. the patie ...	2007	17287696
helminth communities of four commercially important fish species from chetumal bay, mexico.	the relative importance of ecology and evolution as factors determining species richness and composition of the helminth communities of fish is a matter of current debate. theoretical studies use host-parasite lists, but these do not include studies on a temporal or spatial scale. local environmental conditions and host biological characteristics are shown to influence helminth species richness and composition in four fish species (eugerres plumieri, hexanematichthys assimilis, oligoplites sauru ...	2007	17381863
comparison of a manual and an automated method to estimate the number of uterine eggs in anisakid nematodes: to coulter or not to coulter. is that the question?	studies reporting numbers of eggs in vagina and utero in nematodes often give little information of the technique used for the estimations. this situation hampers comparison among studies, because, so far, differences in estimations provided by different techniques have not been assessed. this note examines whether a manual method based on visual counts in aliquots and an automated method using a coulter counter yield equivalent estimations of egg numbers in vagina and utero of 3 anisakid nemato ...	2007	17539430
human pseudoterranovosis, an emerging infection in chile.	fifteen cases of human pseudoterranovosis are reported for chile, representing an emerging parasitic infection in this country caused by larvae of the nematode pseudoterranova sp. our observations also included an outbreak of pseudoterranovosis in 3 of 4 individuals who shared the same raw fish dish (cebiche). most of the cases occurred in adult patients. the main source of infection was from consumption raw or fried marine fish, including hakes (merluccius australis or merlucciuts gayi), pomfre ...	2007	17539437
multiplex pcr for the identification of anisakis simplex sensu stricto, anisakis pegreffii and the other anisakid nematodes.	a multiplex pcr method was established for the rapid identification of anisakis simplex sensu stricto, a. pegreffii, a. physeteris, pseudoterranova decipiens, contracaecum osculatum and hysterothylacium aduncum. the sequence alignment of the internal transcribed spacer 1 region (its-1) between a. simplex s. str. and a. pegreffii showed a high degree of similarity, but only two c-t transitions were observed. to differentiate a. simplex s. str. from a. pegreffii, an intentional mismatch primer wit ...	2008	17962067
application of the pcr-sequence-specific primers for the discrimination among larval anisakis simplex complex.	the fish-borne zoonotic nematode, anisakis simplex, is not a single specie but a complex composed of three sibling species, a. simplex sensu stricto, anisakis pegreffii, and a. simplex c. discrimination among these sibling species have been performed by polymerase chain reaction (pcr)-restriction fragment length polymorphism or sequencing analysis of the internal transcribed spacer (its) region of ribosomal dna (rdna). in the present study, pcr-sequence-specific primers were applied for the disc ...	2008	18239942
molecular detection of sibling species in anisakid nematodes.	the number of sibling species of anisakid nematodes detected over the last two decades has been increased, fuelled by the use of genetic/molecular methodologies. in the present review, we summarize the biological species discovered within most of the nominal species belonging to the genera anisakis, contracaecum and pseudoterranova by the use of allozyme (20-24 loci studied) and recently confirmed by us using mitochondrial cox-2 gene sequence analysis (mtdna cox-2). ecological evidence relating ...	2007	18410072
advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes.	the application of molecular systematics to the anisakid nematodes of the genera anisakis, pseudoterranova and contracaecum, parasites of aquatic organisms, over the last two decades, has advanced the understanding of their systematics, taxonomy, ecology and phylogeny substantially. here the results of this effort on this group of species from the early genetic works to the current status of their revised taxonomy, ecology and evolutionary aspects are reviewed for each of three parasitic groups. ...	2008	18486689
[pseudoterranovosis and sushi].	a case of accidental ingestión of pseudoterranova decipiens is reported, on account of this patient we review main concepts related to this emerging pathology in chile. consumption of raw fish in different preparations such as sushi or sashimi, cebiche and seafood is becoming more and more popular. through intermediary hosts used in the preparation, one can acquire the third stage larval of anisakis simplex or p. decipiens. these parasites frequently cause gastritis, acutely after the consumptio ...	2008	18580998
epidemiology and control prospects of foodborne parasitic zoonoses in the european union.	in the 27 member states of the european union, zoonotic parasites transmitted by food are circulating with different prevalence according to the country, the environmental conditions, the human behaviour, and the socio-economic level. foodborne parasites can be divided in two main groups according to the way of transmission to humans. these foodborne parasites reach the human beings through the consumption of raw infected food such as muscle tissues of different animal species (toxoplasma gondii ...	2008	18693552
metazoan parasite infracommunities of the toadfish aphos porosus (pisces: batrachoidiformes) in central chile: how variable are they over time?	the present study examines variation in parasite community characteristics of the toadfish aphos porosus across sampling years. we analyzed and compared the species composition and numeric descriptors of the parasite infrapopulations and infracommunities in 101 fish collected during the springs of 1999, 2006, and 2007 from rocky intertidal ponds in el tabo, central chile. parasites were found in 94.1% of specimens necropsied. in total 5,532 parasites were collected, representing 12 taxa, of whic ...	2009	19115784
the anisakis simplex ani s 7 major allergen as an indicator of true anisakis infections.	ani s 7 is currently the most important excretory/secretory (es) anisakis simplex allergen, as it is the only one recognized by 100% of infected patients. the allergenicity of this molecule is due mainly to the presence of a novel cx(17-25)cx(9-22)cx(8)cx(6) tandem repeat motif not seen in any previously reported protein. in this study we used this allergen as a model to investigate how es allergens are recognized during anisakis infections, and the usefulness of a recombinant fragment of ani s ...	2009	19438600
additional records of metazoan parasites from caribbean marine mammals, including genetically identified anisakid nematodes.	studies of marine mammal parasites in the caribbean are scarce. an assessment for marine mammal endo- and ectoparasites from puerto rico and the virgin islands, but extending to other areas of the caribbean, was conducted between 1989 and 1994. the present study complements the latter and enhances identification of anisakid nematodes using molecular markers. parasites were collected from 59 carcasses of stranded cetaceans and manatees from 1994 to 2006, including globicephala macrorhynchus, kogi ...	2009	19582477
the metazoan parasite communities of the argentinean sandperch pseudopercis semifasciata (pisces: perciformes) and their use to elucidate the stock structure of the host.	the use of parasites as biological tags allowed the identification of 3 stocks of argentinean sandperch, pseudopercis semifasciata (cuvier), in the argentine sea. a total of 100 specimens caught in 3 zones: villa gesell (37 degrees 15's, 57 degrees 23'w; n=20), miramar (38 degrees 03's, 57 degrees 30'w-38 degrees 44's, 58 degrees 44'w; n=30) and península valdes (42 degrees 00'-42 degrees 45's; n=50), were examined and 28 parasite species were found, 15 of them being new host records. both univa ...	2009	19627631
cold tolerance in sealworm ( pseudoterranova decipiens) due to heat-shock adaptations.	third-stage larvae of pseudoterranova decipiens commonly infect whitefish such as cod, and the parasite can be transferred to humans through lightly prepared (sushi) meals. because little is known about the nematode's cold tolerance capacity, we examined the nematode's ability to supercool, and whether or not cold acclimation could induce physiological changes that might increase its ability to tolerate freezing conditions. even if third-stage pseudoterranova decipiens larvae have some supercool ...	2009	19627634
risk factors for human anisakis infection and association between the geographic origins of scomber japonicus and anisakid nematodes.	it has been reported that nearly all cases of anisakiasis in japan are caused by anisakis simplex sensu stricto. to elucidate this further, we investigated the presence of anisakis type i larvae and pseudoterranova decipiens in 218 scomber japonicus fish collected from the seas of japan. anisakis type i larvae were detected in 74.3% (162/218) of the fish, and 99.8% of the anisakis type i larvae comprised a. simplex sensu stricto and anisakis pegreffii. molecular identification techniques were us ...	2010	19892425
sensitive detection and quantification of anisakid parasite residues in food products.	anisakids are nematodes whose larval stages are often present in fish, molluscs, and crustaceans. members of the family anisakidae belonging to the genera anisakis and pseudoterranova are implicated in human infections caused by the consumption of raw or undercooked fish. adequate cooking will kill anisakid larvae, however, killed or inactivated larvae can still cause sensitization and immunoglobulin e-dependent hypersensitivity in human. this work describes the development of dna-based tests to ...	2010	19911915
direct competitive enzyme-linked immunosorbent assay (elisa) for rapid screening of anisakid larvae in seafood.	anisakid larvae are one of the most important pathogenic parasites in marine products; however, simple and rapid analytical techniques for them are still very limited. in this research, based on specific rabbit polyclonal antibodies which were raised against crude extracts of anisakis larvae, purified by protein a affinity chromatography and labeled with horseradish peroxidase, a direct competitive enzyme-linked immunosorbent assay (elisa) was developed and validated for detection of anisakid la ...	2010	20355125