Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| morphology-associated expression nicotinamide adenine dinucleotide-dependent glutamate dehydrogenase in mucorracemosus. | the in vivo regulation of glutamate dehydrogenase (gdh) was studied in mucor racemosus as a function of nutritional conditions and morphological state. both nicotinamide adenine dinucleotide (nad) and nicotinamide adenine dinucleotide phosphate (nadp)-dependent gdh activities were found. the effect of carbon and nitrogen source on the specific activity of the nad-dependent gdh suggests that its role is primarily catabolic. the nad-dependent activity was generally an order of magnitude greater in ... | 1979 | 35513 |
| respiratory capacity, cyclic adenosine 3',5'-monophosphate, and morphogenesis of mucor racemosus. | a variety of cultural conditions were examined to determine the relationship between respiratory capacity and the growth of mucor racemosus in the yeast and mycelial form. the results show that both yeasts and hyphae can develop when the respiratory capacity is low (e.g., in n2). in addition, the yeast form of the fungus could be grown in air in the presence of cyclic adenosine 5'-monophosphate with high respiratory rates characteristic of air-grown mycelia. these results indicate that their is ... | 1975 | 170243 |
| cyclic guanosine 3',5'-monophosphate in the dimorphic fungus mucor racemosus. | the dimorphic fungus mucor racemosus was found to contain the cyclic nucleotide guanosine 3',5'-monophosphate (cgmp). approximately equivalent amounts of the compound were found in ungerminated spores, yeastlike cells, and mycelia. germinating spores contained severalfold higher amounts of cgmp than the other cell forms. cgmp levels did not change significantly during the morphogenetic conversion of yeast to mycelia. added exogenous cgmp or the dibutyryl derivative did not influence cell morphol ... | 1976 | 176140 |
| control of cyclic adenosine 3',5'-monophosphate levels by depolarizing agents in fungi. | it has been reported that diverse treatments which depolarize the plasma membrane of neurospora crassa produce rapid increases in cyclic adenosine 3',5'-monophosphate (cyclic amp) levels. in the current study, membrane active antibiotics, which are known or putative depolarizing agents, were found to produce similar cyclic amp increases, not only in n. crassa, but also in the distantly related fungi saccharomyces cerevisiae and mucor racemosus. uncouplers of oxidative phosphorylation, which have ... | 1979 | 220213 |
| changing pattern of cyclic amp-binding proteins during germination of mucor racemosus sporangiospores. | interation of cyclic amp with a profoundly changing pattern of specific binding proteins was shown during aerobic germination of sporangiospores from the fungus mucor racemosus. 32p-labeled 8-azido-cycli amp, an analogue of cyclic amp that forms a covalent linkage with the binding proteins under u.v. light, was used as the ligand. binding proteins carrying this photoaffinity label were separated by polyacrylamide-gel electrophoresis and identified by radioautography. equibiltrium dissociation co ... | 1979 | 228658 |
| pyruvate kinase isozymes of mucor racemosus: control of synthesis by glucose. | a variety of cultural conditions were examined to determine the relationship between pyruvate kinase isozyme patterns and morphology in mucor racemosus. the results indicate that m. racemosus has two isozymes of pyruvate kinase, form a and form b, which are clearly separable on ion-exchange columns (diethylaminoethyl-cellulose). addition of glucose to cultures growing on amino acids in air resulted in the induction of form a and the termination of form b synthesis. cycloheximide added at the sam ... | 1977 | 233718 |
| control of beta-glucosidase synthesis in mucor racemosus. | the beta-glucosidase of mucor racemosus was shown to be synthesized when the organism was grown in the presence of such diverse carbon sources as glycerol, lactate, xylose, ribose, alpha-methylglucoside, alpha-phenylglucoside, maltose, and cellobiose. enzyme synthesis was strongly repressed in the presence of hexoses. in addition, exogenous cyclic adenosine 3',5'-monophosphate (camp) resulted in enzyme repression. when camp was added exogenously after enzyme activity had accumulated, a reversibl ... | 1977 | 233721 |
| ribosomal proteins of the dimorphic fungus, mucor racemosus. | ribosomal proteins of the dimorphic fungus mucor racemosus were isolated and characterized by 2-dimensional gel electrophoresis. proteins from ribosomes of the yeast and mycelial phase were compared, and were found to be qualitatively indistinguishable. the only consistent difference in the patterns of proteins was in a protein of the 40s subunit, s-6. this protein was phosphorylated in yeast and hyphae forms, but not in asexual sporangiospores. studies on protein s-6 showed that it contained 3 ... | 1979 | 292824 |
| effect of some medicaments on the oxygen uptake of the spores of some soil fungi. | nystatin, riboflavin, ascorbic acid, captan, indoleacetic acid, and colchicin were tested in two concentrations for their effect on the oxygen uptake of the spores of mucor racemosus, cladosporium tenuissimum, cochliobolus spicifer, and macrophomina phaseoli. nystatin and captan inhibited the oxygen uptake of the spores of all fungi used, and this inhibitory effect increases with increasing concentration. on the other hand, riboflavin, ascorbic acid, indoleacetic acid, and colchicin were found t ... | 1979 | 494856 |
| effect of volatile substances from origanum majorana and ocimum basilicum on spore respiration and germination of some soil fungi. | oxygen uptake by the spores of fusarium moniliforme, f. oxysporum, f. semitectum, f. solani, mucor racemosus and trichoderma viride was increased in the presence of volatile substances extracted from origanum majorana and ocimum basilicum. this increase was greater in the presence of volatile substances from o. basilicum than o. majorana, except in the case of f. semitectum where the reverse was true. a drop in the rq of all the germinating spores was observed in the presence of these substances ... | 1978 | 570540 |
| location of protein synthesis during morphogenesis of mucor racemosus. | the intracellular location of active protein synthesis was examined during the emergence of germ tubes from both sporangiospores and yeast-phase cells of mucor racemosus. it was determined that protein synthesis occurs in all regions of the cell and not preferentially at the growing tip. | 1978 | 618844 |
| regulation of translation rate during morphogenesis in the fungus mucor. | the present study was undertaken in order to elucidate the molecular mechanisms responsible for regulating changes in the specific rate of protein synthesis during the yeast-to-hyphae morphogenesis in the fungus, mucor racemosus. the distribution of ribosomes between active polysomes and monosomes and inactive subunits was determined by means of pulse-labeling and density gradient fractionation techniques. the percentage of ribosomes active in protein synthesis was observed to decrease throughou ... | 1978 | 623731 |
| glucose metabolism and dimorphism in mucor. | mucor racemosus fermented glucose to ethanol, carbon dioxide, and glycerol. when this fungus was grown anaerobically in either the yeast or mycelial form, the catabolism of glucose was very similar. yeast cells shifted to aerobic conditions maintained a high flux of glucose carbon through the glycolytic and pentose phosphate pathways. mycelial cells grown aerobically catabolized glucose in a manner consistent with a respiratory metabolism. although there was no consistent pattern of glucose meta ... | 1978 | 641009 |
| spheroplast fusion and heterokaryon formation in mucor racemosus. | heterokaryons of mucor racemosus were produced by fusion of spheroplasts from two auxotrophic strains of the fungus. germinated sporangiospores were converted to spheroplasts by using commercial chitinase and myxobacter al-1 chitosanase. spheroplasts from the auxotrophic strains were mixed in a buffered ca(no3)2 solution and fusion occurred. after cell wall regeneration, prototrophs were isolated. the frequency of heterokaryon formation was 1.45 x 10(-4). prototrophic isolates segregated parenta ... | 1978 | 649570 |
| regulation of macromolecular synthesis during hyphal germ tube emergence from mucor racemosus sporangiospores. | protein and rna syntheses were examined during hyphal germ tube emergence from sporangiospores of a dimorphic phycomycete, mucor racemosus. both classes of macromolecules were synthesized immediately upon introduction of the dormant sporangiospores into nutrient medium. the specific rates of synthesis of both protein and rna accelerated during initial germ tube emergence and reached a maximum when the emergence of new germ tubes ended. the specific rates of synthesis later decreased during furth ... | 1978 | 649573 |
| rna synthesis during morphogenesis of the fungus mucor racemosus. | 1978 | 727853 | |
| protein synthesis during morphogenesis of mucor racemosus. | cells of mucor racemosus were labeled with l-[(14)c]leucine during the yeast-to-hyphae morphogenesis that follows a change of atmosphere from co(2) to air. pulse-labeling kinetics and the steady-state accumulation of incorporated l-[(14)c]leucine were determined throughout the period of cellular differentiation. we determined that the l-[(14)c]leucine was taken up by all forms of the organism, was not altered from the form of l-leucine, and was incorporated exclusively into protein. the intracel ... | 1977 | 914775 |
| volatile factor involved in the dimorphism of mucor racemosus. | both hyphal and yeastlike development of mucor racemosus and m. rouxii were demonstrated under 100% n2. under standardized conditions in yeast extract-peptone-glucose medium, the morphology depended on the n2 flow rate and not on the glucose concentration. the effect was related to the rate of flushing of the atmosphere over the culture medium. the results indicate that a volatile compound produced by mucor is involved in morphogenesis. | 1976 | 947889 |
| the effect of volatile and gaseous metabolites of swelling seeds on germination of fungal spores. | effects of volatile and gaseous metabolites of swelling seeds of pea, bean, wheat, corn cucumber, tomato, lentil, carrot, red papper and lettuce on germination of spores of five genera of fungi were found to depend rather on the fungal than on the plant genus. germination of spores of botrytis cinerea, mucor racemosus and trichoderma viride was most severely inhibited. spores of verticillium dahliae were less sensitive and germination of spores of fusarium oxysporum was inhibited only in two cas ... | 1975 | 1176038 |
| activation of the complement sequence by extracts of bacteria and fungi associated with hypersensitivity pneumonitis. | complement (c) consumption by antigens associated with hypersensitivity pneumonitis was investigated. micropolyspora faeni in the presence of precipitating antibodies consumed c in a manner similar to the bsa-anti bsa system. an extract of m. faeni was shown to consume c and convert c3pa to c3a in the absence of detectable antibodies. in addition, three species of aspergillus and two strains of thermoactinomyces vulgaris were also shown to have the same activity with respect to c3pa. mucor racem ... | 1976 | 1262608 |
| formation of antigenic extracellular polysaccharides by selected strains of mucor spp., rhizopus spp., rhizomucor spp., absidia corymbifera and syncephalastrum racemosum. | in this study, polyclonal igg antibodies raised against extracellular polysaccharides (eps) of mucor racemosus were characterised as almost specific for moulds belonging to the order of mucorales. cross-reactivity in the elisa could be observed only towards the yeast pichia membranaefaciens. eps were isolated from various cultures of m. hiemalis growing on six different carbon sources and two nitrogen sources, with ratios varying from 0.13 to 0.44 relative to the amount of biomass. other strains ... | 1992 | 1416915 |
| detection of fungal carbohydrate antigens by high-performance immunoaffinity chromatography using a protein a column with covalently linked immunoglobulin g. | fungal carbohydrate antigens were analysed by high-performance immunoaffinity chromatography (hpiac) with immunoglobulin g (igg) antibodies raised against extracellular polysaccharides of mucor racemosus. the protein a-igg complex was covalently bound with dimethyl pimelimidate, which enabled the use of strong acidic buffers to release the tightly bound antigens from the column. prior to pulsed-amperometric detection, an anion-micromembrane suppressor was used to raise the ph of the effluent to ... | 1992 | 1487517 |
| expression of a gene family in the dimorphic fungus mucor racemosus which exhibits striking similarity to human ras genes. | sporulation, spore germination, and yeast-hypha dimorphism in the filamentous fungus mucor racemosus provide useful model systems to study cell development in eucaryotic cells. three ras genes (mras1, mras2, and mras3) from m. racemosus have been cloned, and their nucleotide sequences have been determined. the predicted amino acid sequences and the sizes of the three mras proteins exhibit a high degree of similarity with other ras proteins, including that encoded by h-ras, which have been implic ... | 1990 | 1701021 |
| developmental regulation of cup gene expression through dna methylation in mucor spp. | inserts which carried the cup gene from saccharomyces cerevisiae or mucor racemosus were used as hybridization probes to measure the methylation state and expression of the cup gene from mucor rouxii at different stages of growth. it was observed that the fungus contains a cup multigene family. all the cup genes were present in a hypermethylated dna region in nongrowing and isodiametrically growing spores and were not transcribed at these stages. after germ tube emergence, cup genes became demet ... | 1992 | 1729231 |
| purification and characterization of a developmentally regulated carboxypeptidase from mucor racemosus. | a developmentally regulated carboxypeptidase was purified from hyphae of the dimorphic fungus mucor racemosus. the enzyme, designated carboxypeptidase 3 (cp3), has been purified greater than 900-fold to homogeneity and characterized. the carboxypeptidase migrated as a single electrophoretic band in isoelectric focusing polyacrylamide gel electrophoresis (page), with an isoelectric point of ph 4.4. the apparent molecular mass of the native enzyme was estimated by gel filtration to be 52 kda. sodi ... | 1992 | 1729237 |
| sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast candida albicans. | two candida albicans genes that encode the protein synthesis factor elongation factor 1 alpha (ef-1 alpha) were cloned by using a heterologous tef1 probe from mucor racemosus to screen libraries of c. albicans genomic dna. sequence analysis of the two clones showed that regions of dna flanking the coding regions of the two genes were not homologous, verifying the presence of two genes, called tef1 and tef2, for ef-1 alpha in c. albicans. the coding regions of tef1 and tef2 differed by only five ... | 1990 | 2180914 |
| detection of chitin deacetylase activity after polyacrylamide gel electrophoresis. | mucor racemosus and rhizopus nigricans were used as sources of chitin deacetylases. crude protein extracts were subjected to polyacrylamide gel electrophoresis at ph 8.9 (davis system) or 4.3 (reisfeld system) under native conditions. after electrophoresis, an overlay gel containing 0.1% (w/v) glycol chitin as substrate was incubated in contact with the separation gel. chitin deacetylase activity was revealed by uv illumination with a transilluminator after staining for 5 min in 0.01% (w/v) calc ... | 1990 | 2281870 |
| occurrence of zoosporic and terrestrial fungi in some ponds of kharga oases, egypt. | thirty-nine species belonging to 13 genera of zoosporic fungi were collected from 33 water samples on sesame and hemp seeds as baits at 22 degrees c. five genera were isolated in high occurrence: allomyces (2 species), achlya (9 species), dictyuchus (4 species), pythium (5 species) and saprolegnia (5 species). aphanomyces (4 species) and isoachlya (2 species) were of moderate frequencies. the remaining six genera were less frequent: anisolpidium, brevilegnia, calyptralegnia, leptolegnia, pythiop ... | 1990 | 2384873 |
| primary and secondary structure of the 25s rrna from the dimorphic fungus mucor racemosus. | a 9.76 kb ribosomal dna repeat unit from the nuclear genome of the dimorphic fungus mucor racemosus (zygomycetes) was identified using a hybridization probe from the yeast saccharomyces cerevisiae (ascomycetes). this material was cloned in escherichia coli plasmids as four overlapping pieces and mapped with respect to cleavage sites for 12 restriction endonucleases. the nucleotide sequence of the complete 25s rrna gene and flanking regions was determined. the 5' and 3' ends of the structural gen ... | 1990 | 2390785 |
| role of lysine methylation in the activities of elongation factor 1 alpha. | previous work in our laboratory has demonstrated that 19% of the lysine residues in the protein synthesis elongation factor (ef-1 alpha) are methylated when the factor is purified from the mycelial form of the fungus mucor racemosus. however, the same factor, when purified from spores of m. racemosus, is largely unmethylated. despite its wide-spread occurrence in a great number of basic proteins, the functional significance of lysine n-methylation remains poorly understood. spore and mycelial fo ... | 1989 | 2512854 |
| structure of a ribosomal protein gene in mucor racemosus. | as an extension of our analysis of the translational apparatus of mucor racemosus we have isolated a gene encoding a ribosomal protein of mucor. based on a method developed for s. cerevisiae, we identified by hybrid selection and in vitro translation a lambda-charon 4a clone containing the genomic copy of a mucor ribosomal protein. the gene consisted of two exons of 57 and 387 nucleotides. the two exons were separated by an 131 nucleotide intron. the processed transcript was 714 nucleotides in l ... | 1989 | 2685758 |
| microbial transformations of steroids--iv. 6,7-dehydrogenation; a new class of fungal steroid transformation product. | microbial steroid dehydrogenation is quite common. the reaction seems to occur mainly in bacteria and usually results in hydrogen abstraction from positions c(1)-c(2) and/or c(4)-c(5) with occasional aromatisation of ring a. we have screened large numbers of fungal cultures for their ability to monohydroxylate steroids at unusual sites and in the course of our investigations we have identified seven fungal strains capable of dehydrogenating ring b of progesterone and androstenedione at positions ... | 1989 | 2770300 |
| three genes for the elongation factor ef-1 alpha in mucor racemosus. | we cloned three genes from mucor racemosus coding for protein synthesis elongation factor 1 alpha (ef-1 alpha). a 110-base-pair (bp) ef-1 alpha-specific cdna clone was identified by hybrid-selected translation. the nucleotide sequence of the cdna showed significant homology to a region of the saccharomyces cerevisiae genes for ef-1 alpha (tef1 and tef2). the cdna was used to isolate an 850-bp ecori genomic dna fragment containing a portion of the ef-1 alpha gene. screening of a lambda/m. racemos ... | 1986 | 2946933 |
| the primary structure and the functional domains of an elongation factor-1 alpha from mucor racemosus. | we have determined the complete nucleotide sequence for tef-1, one of three genes coding for elongation factor (ef)-1 alpha in mucor racemosus. the deduced ef-1 alpha protein contains 458 amino acids encoded by two exons. the presence of an intervening sequence located near the 3' end of the gene was predicted by the nucleotide sequence data and confirmed by alkaline s1 nuclease mapping. the amino acid sequence of ef-1 alpha was compared to the published amino acid sequences of ef-1 alpha protei ... | 1986 | 3021762 |
| expression of three genes for elongation factor 1 alpha during morphogenesis of mucor racemosus. | three genes, tef-1, -2, and -3, encode elongation factor 1 alpha in mucor racemosus. neutral and alkaline s1 nuclease analyses revealed that the genetic organization is unique for each of the genes. the number and size of the intervening sequences vary in these closely related genes, which suggests that complex genetic rearrangements gave rise to the elongation factor 1 alpha gene family. nucleotide sequence data from restriction fragments isolated from the 5' and 3' ends of tef-2 and -3 confirm ... | 1987 | 3037339 |
| microorganisms capable of metabolizing the herbicide metolachlor. | we screened several strains of microorganisms and microbial populations for their ability to mineralize or transform the herbicide metolachlor [2-chloro-n-(2-ethyl-6-methylphenyl)-n-(2-methoxy-1-methylethyl)-acetami de] because such cultures would potentially be useful in the cleanup of contaminated sites. although we used various inocula and enrichment culture techniques, we were not able to isolate microorganisms that could mineralize metolachlor. however, strains of bacillus circulans, bacill ... | 1987 | 3105457 |
| mycoflora of air-conditioners dust from riyadh, saudi arabia. | using the hair baiting technique, 6 genera and 14 species were collected on sabouraud's dextrose agar from 37 dust samples from air-conditioners. the most common fungi were chrysosporium tropicum, c. indicum, c. keratinophilum, aspergillus flavus followed by acremonium strictum and scopulariopsis brevicaulis. using the dilution-plate method, 26 genera and 52 species were collected from 37 dust samples on glucose-(23 genera and 45 species) and cellulose-(18 genera and 34 species) czapek's agar at ... | 1988 | 3236219 |
| cycloheximide efflux in antibiotic-adapted cells of the fungus mucor racemosus. | mucor racemosus cells adapted to either cycloheximide or trichodermin were approximately 40-fold more resistant to cycloheximide than nonadapted cells. ribosomes isolated from adapted and nonadapted cells were equally sensitive to cycloheximide in an in vitro poly(u) translation assay. there was no detectable modification of cycloheximide by adapted cells. uptake of drug by nonadapted and adapted cells was characterized by a rapid initial accumulation during the first 2 min of incubation with [3 ... | 1988 | 3364951 |
| human serum antibodies to extracellular polysaccharides (eps) of moulds. | the presence of antibodies to extracellular polysaccharides (eps) of moulds in sera of healthy subjects (n = 125) was determined. antibodies against the eps of penicillium digitatum, mucor racemosus, cladosporium cladosporioides, fusarium moniliforme and botrytis tulipae were found in relatively high amounts in all sera. no effect of age on antibodies present could be demonstrated. antibodies against each of the eps tested were only neutralized by the homologous eps and by eps of moulds belongin ... | 1988 | 3379539 |
| biosynthesis of thiamin. different biosynthetic routes of the thiazole moiety of thiamin in aerobic organisms and anaerobic organisms. | the nitrogen atom of glycine was incorporated into the thiazole moiety of thiamin in the aerobic microorganisms bacillus subtilis, pseudomonas putida, saccharomyces cerevisiae, mucor racemosus, neurospora crassa, and emericella nidulans. it was not incorporated in the case of the facultative anaerobic microorganisms escherichia coli and enterobacter aerogenes, which, however, did incorporate the nitrogen atom of tyrosine. these results show that aerobic microorganisms and facultative anaerobic m ... | 1987 | 3566774 |
| purification and properties of extracellular polysaccharide (eps) antigens produced by different mould species. | extracellular polysaccharide (eps) antigens produced by different mould species were purified and partially characterized. purification included (nh4)2so4 treatment, sepharose cl-4b column chromatography and con a-sepharose chromatography. the eps of penicillium digitatum, mucor racemosus and cladosporium cladosporioides showed high antigenic capacities. immunologically the eps were partially genus-specific, but cross-reactivity was observed. the eps antigens produced by species of penicillium, ... | 1987 | 3571038 |
| purification and properties of two isozymes of pyruvate kinase from mucor racemosus. | the dimorphic phycomycete mucor racemosus was found to contain up to five electrophoretic forms of pyruvate kinase (atp: pyruvate 2-o-phosphotransferase, ec 2.7.1.40) depending on growth conditions. m. racemosus hyphal cells grown on glutamic acid as the carbon source contained only the fastest electrophoretic form, designated pk1, while yeast cells grown on glucose contained only the slowest electrophoretic form, pk5. intermediate electrophoretic forms pk2, pk3, and pk4 as well as pk1 and pk5 w ... | 1987 | 3611022 |
| regulation of gene expression during aerobic germination of mucor racemosus sporangiospores. | the pool of mrna stored in dormant sporangiospores of mucor racemosus and expressed during early germination in air has been investigated. total rna was extracted from dormant and germinating spores and translated in a cell-free rabbit reticulocyte system containing l-[35s]methionine. isotopically labelled in vitro translation products were analysed by page and autoradiography and were compared with labelled proteins synthesized in vivo at the same stages of development. this comparison revealed ... | 1987 | 3655721 |
| mycoflora and trichothecene toxins of paddy grains from egypt. | 120 species and 38 genera were collected from 64 samples of paddy grains on glucose- and cellulose-czapek's agar at 28 degrees c. the total count of glycophilic and cellulose-decomposing fungi fluctuated between 216-29760; and 124-11320 colonies/g paddy grains on the two media, respectively. on glucose agar, the most common species were aspergillus niger, a. flavus, a. sydowi, a. terreus, a. fumigatus, a. ochraceus, penicillium chrysogenum, p. corylophilum, fusarium oxysporum, alternaria alterna ... | 1987 | 3696188 |
| sequence analysis of the ef-1 alpha gene family of mucor racemosus. | our previous studies have shown that mucor racemosus possesses three genes (tef-1, -2 and -3) for ef-1 alpha, and that all three genes are transcribed. however, the level of transcription varies markedly between the three genes, with tef-1 mrna levels being approximately two fold higher than tef-3 and 6 fold higher than tef-2. we have now completed the dna sequence of both strands of all three genes and have found that these genes are highly homologous. tef-2 and tef-3 are more similar to each o ... | 1987 | 3697088 |
| trichodermin esterase activity and trichodermin resistance in mucor racemosus. | mucor racemosus exhibited inducible phenotypic resistance toward the protein synthesis inhibitor trichodermin. induction of resistance was elicited by exposure to trichodermin or to cycloheximide. both adapted and nonadapted cells took up [14c]trichodermin from the medium. trichodermin was found to be rapidly deacetylated to trichodermol upon entering the cell. adapted cells deacetylated the drug more rapidly than nonadapted cells both in vivo and in vitro. the trichodermol resulting from deacet ... | 1986 | 3707105 |
| effect of oxygen on morphogenesis and polypeptide expression by mucor racemosus. | the morphology of mucor racemosus in cultures continuously sparged with nitrogen gas was investigated. when appropriate precautions were taken to prevent oxygen from entering the cultures, the morphology of the cells was uniformly yeastlike irrespective of the n2 flow rate. when small amounts of oxygen entered the cultures the resulting microaerobic conditions evoked mycelial development. polypeptides synthesized by aerobic mycelia, microaerobic mycelia, anaerobic yeasts, and yeasts grown in a c ... | 1985 | 3934135 |
| mycotoxins producing fungi and mycoflora of air-dust from taif, saudi arabia. | using the dilution plate method, 70 species and 31 genera were collected from 20 dust samples on glucose (28 genera and 64 species) and cellulose czapek's agar (22 genera and 46 species) at 28 degrees c. the most common fungi were aspergillus niger, a. flavus, a. flavus var. columnaris, phoma glomerata, fusarium oxysporum, penicillium chrysogenum and mucor racemosus; and a. nidulans, phoma humicola, drechslera spicifera and stachybotrys chartarum on the two media, respectively. toxicity test sho ... | 1985 | 3935928 |
| dispersed repetitive dna sequence of mucor racemosus. | a dispersed repetitive dna sequence has been identified within the genome of the fungus mucor racemosus. recombinant phage clones, as well as a plasmid harboring the sequence, have been isolated. examination of cloned fragments comprising part of the repetitive sequence has led to a partial characterization of the element. the sequence has been detected in other mucor species, and although the apparent number and chromosomal position of the repetitive sequence vary from strain to strain, it is c ... | 1985 | 3980442 |
| regulation of protein synthesis factor ef-1 alpha in mucor racemosus. | the protein synthesis elongation factor ef-1 alpha of mucor racemosus hyphae contained eight or nine methylated amino acids per molecule, whereas the factor from sporangiospores was nonmethylated. during the course of spore germination, the specific activity of the factor in crude extracts increased sixfold. this increase in activity was accompanied by a constant level of ef-1 alpha-specific mrna and a constant level of ef-1 alpha protein. methylation of the protein, however, accelerated during ... | 1985 | 4000119 |
| inducible phenotypic multidrug resistance in the fungus mucor racemosus. | the dimorphic fungus mucor racemosus exhibited a single-step, inducible resistance to cycloheximide, trichodermin, and amphotericin b. cells adapted to inhibitory levels of the antibiotics after 12 to 40 h. the adaptation involved all the cells in the population and was not the result of the selection of resistant mutants. adaptation to one drug provided cross resistance to other, dissimilar drugs. resistance was lost within several generations of growth in the absence of the inhibitors. | 1985 | 4026262 |
| respiratory-competent conditional developmental mutant of mucor racemosus. | a conditional developmental mutant of mucor racemosus which is capable of oxidative energy metabolism is described. unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. the high fermentative activity and yeast morphology under these conditions correlated well with maximal expression of glycolytic enzymes and with expression of some polypeptides characteristic of anaerobic growth. aerobic growth of th ... | 1985 | 4066610 |
| cyclic adenosine 3',5'-monophosphate and morphogenesis in mucor racemosus. | yeastlike cells of mucor racemosus grown under 100% co(2) underwent morphogenesis to hyphae after exposure to air. the addition of dibutyryl cyclic adenosine monophosphate (dbcamp) to yeastlike cultures inhibited this morphogenesis in media containing 2% glucose. the maintenance of uniformly spherical, budding cells required 1 mm dbcamp in a defined medium containing casamino acids, and 3 mm dbcamp in a medium containing yeast extract and peptone. at these concentrations, dbcamp also induced yea ... | 1974 | 4359645 |
| role of amino acids and endogenous protein in the germination of mucor racemosus sporangiospores. | the role of amino acids as triggers of mucor racemosus sporangiospore germination was investigated. no single amino acid was effective as glucose or peptone at triggering germination. germination induced by glucose or peptone was ph-independent, whereas germination induced by glutamate was ph-dependent. the composition of the free amino acid pools of m-spores (those unable to germinate on glutamate) was qualitatively and quantitatively similar to that of c-spores (those capable of germinating on ... | 1982 | 6122713 |
| purification of a soluble and a wall-bound form of beta-glucosidase from mucor racemosus. | beta-glucosidase activity in crude extracts of mucor racemosus exists in a soluble form and in a wall-bound form which sediments at 3,500 x g. the soluble form and a wall-bound form were purified to homogeneity by ammonium sulfate fractionation. deae-sephadex chromatography, and sp-sephadex chromatography. both forms were identical in all parameters measured. each enzyme is a glycoprotein of 91,000 daltons, with an identical amino acid composition and n-terminal amino acid of lysine; both contai ... | 1982 | 6173375 |
| regulation of ornithine decarboxylase during morphogenesis of mucor racemosus. | during the yeast-to-hyphae transition of the dimorphic phycomycete mucor racemosus, there was a 30- to 50-fold increase in the activity of ornithine decarboxylase. increased enzyme activity preceded the emergence of germ tubes and reached a maximum before conversion was completed. subsequently, enzyme levels rapidly declined, despite the continuation of mycelial growth. both putrescine and spermidine blocked the enzyme activity response. protein synthesis was required for the increase in enzyme ... | 1980 | 6245054 |
| the organization of the ribosomal rna genes in the fungus mucor racemosus. | the rdna of mucor racemosus is contained on a 6.4 megadalton repeat unit. two bam h-1 restriction fragments that encompass the entire rdna repeat, as well as two hind iii restriction fragments that lie within the region, have been cloned and analyzed. the rdna unit has been defined with respect to eight restriction endonucleases and the position of the sequences encoding the 25s, 18s, and 5.8s rrna species have been localized. in addition, the 55 rna encoding sequence was found to reside within ... | 1980 | 6253925 |
| relationship between sporulation medium and germination ability of mucor racemosus sporangiospores. | asexual sporangiospores of mucor racemosus produced on a minimal sporulation medium (m spores) germinated only if glucose, mannose or a complex substrate such as peptone, yeast extract or casamino acids was present. once germinated, growth was supported by a wide range of substrates including amino acids, carbohydrates or organic acids. sporangiospores produced on a nutritionally complex sporulation medium (c-spores) germinated on a wide range of carbon sources. c-spore phenotype was pleiotropic ... | 1981 | 6279767 |
| selective effects of two systemic fungicides on soil fungi. | bas 317 00f was not toxic to the total count of fungi after 2 days but was regularly significantly toxic at the three doses after 5, 20 and 40 days and toxic at the low and the high doses after 80 days. in the agar medium, it was toxic to the counts of total fungi. aspergillus, a. terreus, rhizopus oryzae and mucor racemosus at the high dose. only the mycelial growth of trichoderma viride which was significantly inhibited by the three doses when this fungicide was added to the liquid medium. pol ... | 1982 | 6290890 |
| differential gene expression during aerobic germination of mucor racemosus sporangiospores. | evidence is provided suggesting that several modes of differential gene expression operate concomitantly during the first 60 min of germination of mucor racemosus sporangiospores under air. protein synthesis was initiated immediately upon exposure of the spores to nutrient medium and accelerated exponentially throughout the period of observation. all translation during the first 30 min of germination occurred using only preformed stable mrnas as a template. analysis of the protein products synth ... | 1984 | 6480554 |
| temperature and water activity minima for growth of spoilage moulds from meat. | five species of fungi were isolated from mould spoilage on meat other than black spot. 'white spot' colonies yielded chrysosporium pannorum or an acremonium sp.; 'whiskers' colonies yielded thamnidium elegans or mucor racemosus, and blue-green colonies yielded penicillium corylophilum . chrysosporium pannorum was moderately xerotolerant with a minimum growth temperature of -5 degrees c. the acremonium sp. and p. corylophilum showed a similar level of xerotolerance but had a minimum growth temper ... | 1984 | 6725157 |
| composition of the fungal flora of four cereal grains in saudi arabia. | using the grain-plate method and on glucose- czapek 's agar at 28 degrees c, fifty-eight species belonging to 26 genera were collected from barley (42 species and 19 genera), maize (29 species and 16 genera), sorghum (32 species and 17 genera) and wheat grains (42 species and 18 genera). the most frequent genera were aspergillus, penicillium, rhizopus, fusarium, and mucor followed by alternaria, drechslera , and curvularia. from the preceding genera aspergillus flavus, a. niger, penicillium nota ... | 1984 | 6727980 |
| effect of volatile and gaseous metabolites of germinating pea seeds on micromycetes. | differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of mucor racemosus and macroconidia of fusarium oxysporum are described. germination of spores of m. racemosus was inhibited by seed metabolites whereas germination of macroconidia of f. oxysporum was stimulated during the first two days and inhibition occurred only after further two days of germination of the seeds. a pronounced inhibition of germination of spores of both micromyc ... | 1980 | 6769770 |
| growth-rate-dependent adjustment of ribosome function in chemostat-grown cells of the fungus mucor racemosus. | the dimorphic fungus mucor racemosus was grown as a yeast in a chemostat. cellular growth rates were varied over a fourfold range under an atmosphere of n2 and over an eightfold range under co2. under either atmosphere, an increase in the cellular growth rate resulted in increases in (i) the cellular ribosome concentration, (ii) the percentage of ribosomes active in protein synthesis, and (iii) the rate of polypeptide chain elongation. the rate of protein synthesis in this organism can therefore ... | 1982 | 6799491 |
| methylation of elongation factor 1 alpha from the fungus mucor. | a basic protein from the dimorphic fungus mucor racemosus, found to be highly methylated, is shown to be protein synthesis elongation factor 1 alpha. this protein is the most abundant protein in hyphal cells but is less abundant in yeast cells. it is post-translationally methylated with the formation of mono-, di-, and trimethyllysine at as many as 16 sites. nearly 20% of the 44 lysine residues of elongation factor 1 alpha from mycelia are modified while those from sporangiospores are virtually ... | 1982 | 6808511 |
| complementation of the leub6 allele of escherichia coli by cloned dna from mucor racemosus. | a recombinant plasmid, designated pmu leu1, was constructed from mucor racemosus genomic dna and escherichia coli plasmid pbr322. this plasmid complemented the leub6 mutation of e. coli, apparently by suppression. the plasmid contained two hindiii fragments of approximately 3.0 and 1.7 kilobases. neither fragment alone exhibited complementing activity. several proteins were specified by the plasmid, but their role, if any, in complementation is unknown. | 1982 | 7045088 |
| aflatoxin and aflatoxicosis. i. fungal flora of some food and animal feeds with special references to aflatoxin-producing abilities. | a wide range of fungi, amounting to fifty-six species belonging to twenty-two genera, have been recovered from animal feeds and foodstuffs. the most frequent fungi were aspergillus niger, and a. flavus, followed by mucor racemosus, alternaria alternata. rhizopus stolonifer, penicillium corylophilum and p. notatum. three genera were found to be of moderate occurrence, namely, mucor, rhizopus and alternaria. the three following genera were of low occurrence: cladosporium, fusarium, and neurospora. ... | 1982 | 7070483 |
| stored mrna in sporangiospores of the fungus mucor racemosus. | when introduced into nutrient medium under air, the asexual sporangiospores of mucor racemosus germinated with 5 to 8 h, culminating with the emergence of germ tubes. we found that sporangiospores increased 20% in dry weight during the first 60 min of germination, indicating a high degree of synthetic activity. sucrose density gradient analysis of spore extracts revealed that the percentage of ribosomes associated with mrna increased from 22.5% in dormant spores to 85% within 10 min after the ad ... | 1982 | 7076616 |
| glucose-initiated germination of mucor racemosus sporangiospores. | treatments leading to the initiation of germination of mucor racemosus sporangiospores were examined. the results support the hypothesis that glucose is a specific trigger molecule for the initiation of mucor racemosus sporangiospores. glucose and some of the glucose analogues tested could initiate germination, mannose, 3-o-methyl-d-glucose, 5-thio-d-glucose and 6-deoxy-d-glucose being the most effective. the initiation event appeared to depend on the concentration of the initiator, with glucose ... | 1982 | 7077298 |
| lipid synthesis during morphogenesis of mucor racemosus. | lipid synthesis increases coordinately with protein and rna synthesis during morphogenesis of mucor racemosus. the lipid synthesis inhibitor cerulenin can completely block morphogenesis under conditions in which cell growth continues. an increase in phospholipid turnover may be an important correlate to morphogenesis of mucor spp., especially the turnover of phosphotidyl inositol and phosphatidyl ethanolamine. the increase in ornithine decarboxylase, which occurs during morphogenesis, is inhibit ... | 1982 | 7130131 |
| volatile and gaseous metabolites released by germinating seeds of lentil and maize cultivars with different susceptibilities to fusariosis and smut. | the effect of volatile and gaseous metabolites released by germinating seeds of lentil cultivars more and less susceptible to fusariosis on the germination of spores of mucor racemosus, trichoderma viride, verticillium dahliae and botrytis cinerea was found to depend rather on the fungal genus than on the lentil cultivar. however, spores of fusarium oxysporum reacted more sensitively during germination to the presence of exudates of both cultivars, when the more susceptible lentil displayed a st ... | 1980 | 7189738 |
| changes in the rate of chitin-plus-chitosan synthesis accompany morphogenesis of mucor racemosus. | the in vivo differential rates of chitin-plus-chitosan biosynthesis in mucor racemosus were determined under a variety of conditions, leading to yeast cell or mycelial morphology. chitin-chitosan was determined as hot naoh-insoluble radioactivity derived from n-acetyl-d-[1-3h]glucosamine in the medium. control experiments demonstrated that the labeled material possessed the properties of chitin-plus-chitosan. our results indicate that mucor yeasts have a relatively low differential rate of chiti ... | 1981 | 7240089 |
| growth-rate-dependent adjustment of ribosome function in the fungus mucor racemosus. | the dimorphic fungus mucor racemosus was grown at rates between 0.043 and 0.434 doubling/h while maintained as yeasts or at rates between 0.21 and 0.50 doubling/h while maintained as hyphae by altering the composition of the growth medium or the gaseous environment of the cells. yeasts at the higher growth rates contained many more ribosomes than did yeasts at the lower growth rates. they also had a higher percentage of ribosomes active in protein synthesis and a faster rate of polypeptide-chain ... | 1981 | 7316986 |
| physiological control of phosphorylation ribosomal protein s6 in mucor racemosus. | the level of phosphorylation of ribosomal protein s6 increased with accelerating rates of growth and protein synthesis in mucor racemosus. lowered levels of phosphorylation were seen under conditions of metabolic shift-down or the onset of stationary phase, and no phosphorylation was detected in sporangiospores. changing metabolic states, changing intracellular levels of adenosine triphosphatase, and the level of phosphorylation of protein s6 were correlated in m. racemosus. | 1980 | 7353998 |
| high-frequency heterokaryon formation by mucor racemosus. | heterokaryons from mucor racemosus were produced from two auxotrophic strains of the fungus. germlings were converted to spheroplasts by using commercial chitinase and purified myxobacter al-1 chitosanase. spheroplasts from the auxotrophic strains were mixed and fused in solutions of polyethylene glycol and cacl2. under optimal conditions, prototrophic heterokaryons were formed at a frequency of about 5%. | 1980 | 7364713 |
| differential synthesis of polypeptides during morphogenesis of mucor. | the extent of differential gene expression during morphogenesis of mucor racemosus was investigated by two-dimensional polyacrylamide gel electrophoresis of neutral and acidic polypeptides. cellular proteins were labeled with [35s]methionine in cells growing in either the yeast or hyphal form, or in yeast cells undergoing the transition of hyphae. the results showed that of the 400 to 500 polypeptides resolved by electrophoresis, relatively few were specific to one or the other morphological for ... | 1980 | 7364729 |
| s-adenosylmethionine levels and protein methylation during morphogenesis of mucor racemosus. | the intracellular level of s-adenosylmethionine increased as the yeast-phase cells of mucor racemosus were induced to convert to hyphae. this increase correlated well with time course of the conversion in cell type and was independent of the metabolic changes caused by the shift to aerobic conditions. there was no significant change in the intracellular level of spermidine, a polyamine synthesized from putrescine and the propylamine group of s-adenosylmethionine. spermine was not detected. an ex ... | 1980 | 7372569 |
| glyoxylate cycle in mucor racemosus. | the dimorphic phycomycete mucor racemosus was grown in media containing acetate, glutamate, and peptone as carbon sources. the component enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase, were present under these conditions throughout the growth cycles. highest specific activities for each enzyme were found in media with acetate as the carbon source. in an enriched peptone medium containing glucose, neither activity was detected until glucose was exhausted from the medium. t ... | 1980 | 7400097 |
| contribution of selected fungi to the reduction of cyanogen levels during solid substrate fermentation of cassava. | the effect of six individual strains of the dominant microflora in solid substrate fermenting cassava on cyanogen levels was examined. six out of eight batches of disinfected cassava root pieces were incubated for 72 h after inoculation with either of the fungi geotrichum candidum, mucor racemosus, neurospora sitophila, rhizopus oryzae and rhizopus stolonifer, or a bacillus sp., isolated from on-farm fermented cassava flours from uganda. one non-inoculated batch was incubated as a reference. lev ... | 1995 | 7577362 |
| cloning and analysis of cdna encoding an elongation factor 1 alpha from the dimorphic fungus histoplasma capsulatum. | the cdna encoding translation elongation factor 1 alpha (ef-1 alpha) was isolated from the dimorphic fungus, histoplasma capsulatum (hc), an important pathogen of man. a cdna library was probed with the tef1 gene from the fungus mucor racemosus. ten independent clones were isolated, all with similar restriction patterns. the longest clone (1.96 kb) was sequenced. southern blot analysis revealed that the hc tef1 gene was present as a single copy. a single transcript of approx. 2300 nucleotides wa ... | 1995 | 7642125 |
| the production and partial characterization of a monoclonal igg antibody specific for moulds belonging to the order mucorales. | a monoclonal antibody (mab) was raised against extracellular polysaccharides from mucor racemosus after intrasplenic immunization of mice. an indirect elisa and a dot-blot assay were developed with this mab. the igg antibody was found to be very specific for all mould species tested belonging to the order of mucorales, except species belonging to the genus mortierella sensu stricto. no cross-reactions were observed with other moulds or yeasts. the immunoreactivity of the polysaccharides of these ... | 1993 | 7690393 |
| analysis of the gene for the elongation factor 1 alpha from the zygomycete absidia glauca. use of the promoter region for constructions of transformation vectors. | the complete genomic dna sequence was determined for one of the gene for the elongation factor 1 alpha (tef), isolated from the zygomycete absidia glauca. sequence comparison with tef genes from other fungi show the highest similarity to tef-genes of the closely related zygomycete mucor racemosus (sundstrom et al. 1987). southern-blot analysis of genomic dna from a. glauca with the tef gene reveals six chromosomal copies in the genome. in transformation experiments of a. glauca, vector construct ... | 1995 | 7735720 |
| [contamination of archives by filamentous fungi and their evaluation for potential pathogenicity]. | the results of microbiological examinations of dust samples collected from mortgage registers in the court archives are presented. in the samples examined filamentous fungi of penicillium genus were observed as well as cladosporium herbarum, geotrichum candidum, cephalosporium glutineum, mucor racemosus, trichoderma viride and aspergillus niger. filamentous fungi of penicillium genus occurred in the highest concentration accounting for 350,000 cfu/g. the levels of the remaining fungi were lower- ... | 1994 | 7854106 |
| mitochondrial genome of the dimorphic zygomycete mucor racemosus. | mitochondria were isolated from the dimorphic zygomycete mucor racemosus by differential centrifugation. dna from the organelles was purified by cesium chloride-ethidium bromide isopycnic centrifugation. examination of the mitochondrial dna by electron microscopy revealed a circular chromosome approximately 63.8 kbp in circumference. the chromosome was digested with restriction endonucleases and the resulting dna fragments were separated by agarose-gel electrophoresis. electophoretic mobilities ... | 1993 | 8252644 |
| isolation of trichoderma reesei genes highly expressed on glucose-containing media: characterization of the tef1 gene encoding translation elongation factor 1 alpha. | genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, trichoderma reesei. a cdna bank was prepared from glucose-grown fungus, the bank was screened with the same cdna as a probe, and clones giving the strongest signal were isolated. this resulted in the isolation of previously uncharacterized genes. five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mrna population and were clearly more highly expresse ... | 1993 | 8294023 |
| 2-o-methyl-d-mannose residues are immunodominant in extracellular polysaccharides of mucor racemosus and related molds. | in this study, the structure of the immunodominant carbohydrate epitope of the extracellular polysaccharides from mold species belonging to the order mucorales reactive with rabbit igg antibodies was elucidated. an exo-alpha-d-mannanase which was able to abolish the antigenicity of these polysaccharides completely was purified and characterized, and the activity was compared with that of an alpha-d-mannosidase. analysis of the monomeric reaction products after enzymatic treatment revealed the pr ... | 1994 | 8307996 |
| rin, a neuron-specific and calmodulin-binding small g-protein, and rit define a novel subfamily of ras proteins. | cdnas encoding two novel 25 kda ras-like proteins, rit and rin, were isolated from mouse retina using a degenerate pcr-based cloning strategy. using the expressed sequence tag database, human orthologs were also obtained and sequenced. the protein sequences of rit and rin, which are 64% identical, are more similar to each other than to any known ras protein. their closest homologs in the databases are mucor racemosus ras2 and ras3, to which they show approximately 48% identity. rit and rin both ... | 1996 | 8824319 |
| identification of a group-i intron within the 25s rdna from the yeast arxula adeninivorans. | the 25s rdna of the yeast arxula adeninivorans ls3 has been closed from a genomic library and sequenced. this dna could be localized on chromosome 1 from a. adeninivorans and comprised 3790 bp. the dna sequence from this rdna of the strain ls3 is very similar to the 25s rdna of candida albicans (91.7%), saccharomyces cerevisiae (90.5%), schizosaccharomyces pombe (83.8%) and mucor racemosus (79.2%). additionally a 411 bp insertion could be localized within the 25s rdna. this intervening sequence, ... | 1996 | 8905924 |
| [the role of imperfect fungi in etiopathogenesis of allergic rhinitis]. | in this paper the role of imperfect fungi in etiopathogenesis of perennial rhinitis was examined. in a group of 26 patients the concentration of total ige and ige specific of candida albicans, aspergillus fumigatus, alternaria alternata, mucor racemosus and cladosporum herbarum was signified. oversensibility to imperfect fungi was confirmed in 30.8% of patients: to candida albicans in 3.8%, to aspergillus fumigatus in 11.5%, to alternaria alternata in 3.8%, to mucor racemosus in 7.6% and to clad ... | 1998 | 9760768 |
| lovastatin triggers an apoptosis-like cell death process in the fungus mucor racemosus. | the filamentous dimorphic fungus mucor racemosus possesses three ras genes, mras1, 2, and 3, whose expression is correlated to morphogenesis of the fungus. lovastatin, an indirect inhibitor of protein prenylation, altered the processing of mras1 protein, blocked the accumulation of mras3 protein, and caused the mras1/p20 protein complex to disappear in m. racemosus. concurrently it arrested sporangiospore germination, decreased growth rate, caused a loss of cell viability accompanied by cell shr ... | 1998 | 9974223 |
| the antifungal properties of chitosan in laboratory media and apple juice. | the antimicrobial properties of chitosan glutamate, a derivative of chitin, were investigated in laboratory media and apple juice against 15 yeasts and moulds associated with food spoilage in order to assess the potential for using chitosan as a natural food preservative. of the seven strains of filamentous fungi studied, chitosan reduced the growth rate of mucor racemosus at 1 g/l whilst concentrations of 5 g/l were required to completely prevent growth of three strains of byssochlamys spp. on ... | 1999 | 10357275 |
| a mycological investigation of phane, an edible caterpillar of an emperor moth, imbrasia belina. | phane worm (an edible larval stage of the emperor moth imbrasia belina westwood) is an important food source, and its harvesting is an economic activity in rural botswana. when the larva is feeding on leaves and later during processing, phane gets contaminated with fungi from the leaves and soil. we examined 73 jars, each containing approximately 608 g (+/-0.25 g) of processed phane stored under laboratory conditions (temperature range 20 to 24 degrees c and 50 to 80% relative humidity) and comb ... | 2000 | 10643785 |
| biodegradation of pyrene by sediment fungi. | micromycetes were isolated from pahs-contaminated sediment and identified. they were investigated for pyrene degradation (10 mg l-1) in liquid synthetic medium for two days. among the 41 strains isolated, 10 highly degraded pyrene (> 2.4 mg g-1 dry weight): two zygomycetes (mucor racemosus, m. racemosus var. sphaerosporus), 6 deuteromycetes (gliocladium virens, penicillium simplicissimum, p. janthinellum, phialophora alba, p. hoffmannii, trichoderma harzianum), a dematiaceae (scopulariopsis brum ... | 2000 | 10665394 |
| evidence that mras1 and mras3 proteins are associated with distinct cellular functions during growth and morphogenesis in the fungus mucor racemosus. | the filamentous fungus mucor racemosus provides a simple and unique model system for defining the function of individual ras genes in a gene family which is closely related to mammalian ras genes. the current study was designed to investigate the role of mras1 and mras3 in different stages of fungal morphogenesis, including sporangiospore germination, sporulation, and dimorphic transitions. the overall patterns of mras1 and mras3 transcript and protein accumulation were markedly different but, i ... | 1999 | 10669583 |
| in-situ detection of aspergillus fumigatus. | an in-situ hybridisation (ish) technique to detect aspergillus fumigatus in infected tissues was developed in which 568-bp, 333-bp and 154-bp pcr products of the alkaline proteinase gene were employed. dot-blot hybridisation with the 568-bp probe on a membrane containing genomic dna from several different fungi including a. flavus, a. niger, penicillium spp., mucor racemosus or pseudallescheria boydii gave negative results. ish was done on formalin-fixed, paraffin-embedded pulmonary tissues from ... | 2000 | 10707949 |
| biosynthesis of pyridoxine: origin of the nitrogen atom of pyridoxine in microorganisms. | the amide nitrogen atom of glutamine was incorporated into pyridoxine in four eukaryotes, emericella nidulans, mucor racemosus, neurospora crassa and saccharomyces cerevisiae, and two prokaryotes, staphylococcus aureus and bacillus subtilis, but not in the following prokaryotes, pseudomonas putida, enterobacter aerogenes and escherichia coli. on the other hand, the nitrogen atom of glutamate was incorporated into pyridoxine in p. putida, e. aerogenes and e. coli, but not in s. aureus and b. subt ... | 2000 | 10885790 |
| antifungal effects of hydrolysable tannins and related compounds on dermatophytes, mould fungi and yeasts. | a series of hydrolysable tannins and related compounds was evaluated for antifungal activities against filamentous fungi (epidermophyton floccosum; microsporum canis; microsporum gypseum; trichophyton mentagrophytes; trichophyton rubrum; trichophyton tonsurans; trichophyton terrestre; penicillium italicum; aspergillus fumigatus; mucor racemosus; rhizopus nigricans) and opportunistic yeasts (candida albicans; candida glabrata; candidata krusei; cryptococcus neoformans), using the agar dilution me ... | 2000 | 10928561 |
| mycoflora in commercial pet foods. | this article reports on the identification of mycoflora of 21 dry pet foods (12 belonging to dogs and 9 to cats) that corresponded to 8 commercial brands made in argentina and imported. the isolation frequency and relative density of the prevalent fungal genera are compared too. ten genera and fungi classified as mycelia sterilia were identified. the predominant genera were aspergillus (62%), rhizopus (48%), and mucor (38%). the most prevalent among aspergillus was aspergillus flavus followed by ... | 2001 | 11348013 |
| antifungal and cytotoxic effects of methanol extracts of three marine molluscs. | methanol extracts of whole body (melampus ceylonicus), blood (anadara rhombea) and hepatopancreas (sepiella inermis) were screened for antifungal and cytotoxic activity. blood of a. rhombea and hepatopancreas of s. inermis strongly inhibited candida albicans, mucor racemosus and penicillium expansum. a. rhombea appear to exert strong cytotoxic activity against vertebrate erythrocytes. the bacterial pathogens (escherichia coli and vibrio cholarae) were not inhibited by any of the three tested met ... | 2001 | 11349534 |
| liquid chromatography study of pyrene degradation by two micromycetes in a freshwater sediment. | pyrene biodegradation in a freshwater sediment without fungi supply, or inoculated with two sediment micromycetes, mucor racemosus var. sphaerosporus and phialophora alba was studied after 0, 5, 13, 28, 60 and 90 days. the influence of glucose addition was estimated, and a liquid chromatographic method for simultaneous quantitative determination of residual anthracene, fluoranthene and pyrene in the sediment was developed. samples with pahs were extracted in soxhlet with ethyl acetate, and lc an ... | 2001 | 11545519 |