Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [purification and characterization of two peroxidases from hard wheat]. | two peroxidases a and b were purified from a borate buffer extract (ph = 10,4) of durum wheat semolina (triticum durum), var. bidi 17, by chromatography on deae-cellulose, salting out by 3m ammonium sulphate and two chromatographies on cm-cellulose; specific activities of peroxidase a or b were increased 114 or 66 fold. molecular weight, amino acid composition, absorption spectrum, ph optimum, thermal stability and km values differentiate the two enzymes. ion ca++ was shown as an activator of bo ... | 1975 | 237578 |
| [purification of a protein from the seeds of hard-grained wheat (triticum durum) and preparation of its immunospecific serum]. | 1978 | 737068 | |
| intrachromosomal distribution of chromatid aberrations induced by n-methyl-n-nitrosourethane in triticum durum desf. | 1977 | 854044 | |
| on the location of glutamate decarboxylase in the caryopsis of hard wheat (triticum durum) and its activity during early germination. | 1975 | 1112313 | |
| [variability in the fertility of pollen from m2 mutants of spring durum wheat]. | 2013 | 1145741 | |
| [influence of the degree of nonvitreousness on durum wheat semolina characteristics (author's transl)]. | the presence of nonvitreous kernels in durum wheat adversely influences milling yield, protein content and pasta manufacturing quality of semolina. from the results obtained, we can infer that the proportion of these negative effects is related to the degree of nonvitreousness exhibited by the kernels. consequently, to define nonvitreousness and to establish proper methods for its determination, we have to take into consideration not only the percent of nonvitreous kernels but also the degree of ... | 2008 | 1243933 |
| absence of guar efficacy in complex spaghetti meals on postprandial glucose and c-peptide levels in healthy control and non-insulin-dependent diabetes mellitus subjects. | the effects of guar incorporated into a complex spaghetti meal on the glycaemic response in 11 healthy and 6 non-insulin dependent diabetic (niddm) subjects was studied. to this end, subjects consumed spaghetti made of either triticum aestivum or triticum durum wheat with or without 20 g% guar, as part of a complex meal containing 27% fat, 19% protein and 51% carbohydrate. in both the healthy as well as the niddm subjects the incremental integrated postprandial glucose and c-peptide responses af ... | 1992 | 1337059 |
| effect of alpha-gliadin-derived peptides from bread and durum wheat on k562(s) cells. | previous studies suggested that the proteins and peptides that are responsible for coeliac small intestinal lesions, are also able to agglutinate k562(s) cells. on the contrary, peptides from whole gliadins from durum (tetraploid) wheat do not agglutinate these cells. bread wheat alpha-gliadins have been identified as a major toxic fraction in coeliac disease. in the present research, alpha-gliadins were purified from durum and bread wheat cultivars. alpha-gliadin peptides from bread wheat were ... | 1992 | 1392022 |
| nucleotide sequence of a low-molecular-weight glutenin from triticum durum. | 1992 | 1558951 | |
| nucleotide sequence of a cdna encoding a lipid transfer protein from wheat (triticum durum desf.). | 1992 | 1627784 | |
| on the importance of processing conditions, product thickness and egg addition for the glycaemic and hormonal responses to pasta: a comparison with bread made from 'pasta ingredients'. | the importance of processing conditions, product thickness and effect of egg addition for the glycaemic and hormonal responses to pasta was studied. healthy subjects were given test meals with an equivalent amount of available carbohydrate from extruded high-temperature dried spaghetti and three varieties of fresh roll-sheeted linguine (thick, thin, thin with egg) made from the same ingredients (durum wheat, water and monoglycerides). as a reference bread was baked from the same ingredients as i ... | 1991 | 1782920 |
| cellular and humoral responses in coeliac disease. 2. protein extracts from different cereals. | the humoral and cellular immune responses to grain protein extracts from coeliac-toxic and non-toxic cereals were compared by use of a number of elisa and immunoblotting methods and the indirect leucocyte migration inhibition factor (lmif) assay. both adult and child coeliacs had elevated levels of serum antibody to proteins from the coeliac-toxic cereals, namely bread wheat, durum wheat, rye and barley and low levels of proteins from other cereals. using protein blotting techniques, antibody bi ... | 0 | 1819454 |
| nucleotide sequence of a cdna clone encoding the wheat (triticum durum desf.) cm2 protein. | 1991 | 1893104 | |
| characterization of a cdna clone encoding the triticum aestivum l. cm16 protein: homology with the triticum durum desf. sequence. | 1991 | 1893108 | |
| cloning and characterization of a cdna encoding the wheat (triticum durum desf.) cm16 protein. | a triticum durum cdna library prepared from developing endosperm (22 days after flowering (daf] was screened using synthetic oligonucleotide probes covering part of the cm3 and cm16 n-terminal protein sequences. a full-length cdna clone (ptd78) encoding the cm16 protein (chloroform/methanol-soluble protein) was isolated and characterized. to our knowledge this is the first characterization of a clone coding for a wheat cm protein. the cm16 protein is synthesized as a preprotein with a signal pep ... | 1990 | 2102817 |
| effects of corn oil and wheat brans on bile acid metabolism in rats. | high concentrations of colonic bile acids may promote tumor formation. some studies have found that high levels of dietary fat increase fecal bile acid excretion, whereas others report no effect. wheat bran appears to reduce fecal bile acid concentration. this study was conducted to determine the effect of different dietary fat levels and types of wheat bran on bile acid metabolism. rats were fed diets containing either no fiber, 2% cholestyramine (cho) or brans of hard red spring, soft white wi ... | 1990 | 2172490 |
| mycotoxins and toxigenic fungi on cereal grains in western canada. | toxins occasionally present on cereal grains in the field in western canada include ergot alkaloids produced by claviceps purpurea and trichothecenes produced by fusarium species, particularly fusarium sporotrichiodes and fusarium graminearum. ht-2 toxin, t-2 toxin, diacetoxyscirpenol, and deoxynivalenol are the main trichothecenes encountered. during storage of cereals, the predominant toxins and toxigenic fungi are ochratoxin a and citrinin produced by penicillium aurantiogriseum, p. chrysogen ... | 1990 | 2200591 |
| mycotoxin production in amber durum wheat stored at 15 and 19% moisture content. | ochratoxin a and citrinin developed in 11 kg parcels of amber durum wheat at 15% and 19% initial moisture content (mc) exposed to simulated bulk storage in a manitoba granary for 60 weeks between july 1984 and september 1985. other biotic and abiotic variables were monitored throughout the storage period. ochratoxin a reached maximum levels of 11.8 and 0.11 ppm at 19 and 15% initial mc, respectively, during weeks 44-48; citrinin reached levels of 80.0 and 0.65 ppm at these respective moistures d ... | 1990 | 2253806 |
| separation of durum wheat proteins by ultrathin-layer isoelectric focusing: a new tool for the characterization and quantification of low molecular weight glutenins. | an isoelectric focusing method capable of resolving all groups of storage protein of the wheat seed, including the most basic low molecular weight glutenin (lmwg), was developed. ultrathin polyacrylamide gels were used after drying and rehydration with 8 m urea, 50 mm dte and 2.4% carrier ampholytes (ph 4-9). densitometric scanning of the isoelectric focusing gels permitted a more accurate and specific quantitation of lmwg components among various cultivars than patterns based on sodium dodecyl ... | 1990 | 2364926 |
| effects of durum wheat dietary selenium on glutathione peroxidase activity and se content in long-term-fed rats. | a 120-day assay was made of se concentration and glutathione peroxidase se-dependent (gshpx) activity in plasma, erythrocytes and liver of female sprague-dawley rats fed either a torula diet (low se content) or a durum wheat diet providing 28-405 micrograms of se/kg diet. for all groups there was a strong increase for the first 20 days in plasma and liver se; for the remaining period the increase was lower; erythrocyte se increased, as before, in the first 60 days for groups fed 28-200 microgram ... | 1989 | 2742329 |
| detergents as selective inhibitors and inactivators of enzymes. | in order to study the detergent-enzyme interaction and to clarify whether such an interaction produces specific or non-specific effects, we investigated the action of natural and synthetic detergents on enzymatic systems of different levels of complexity (crystalline enzymes, crude homogenates, organ preparations, organisms in toto i.e. rats and germinating seeds). the enzyme-detergent interaction was examined both as a time-independent phenomenon (inhibition) and as a time-dependent phenomenon ... | 1985 | 2937071 |
| characterization and quantification of low molecular weight glutenins in durum wheats. | durum wheat proteins have been considered as a model because of the very clear-cut relationship previously evidenced between the electrophoretic type '42' or '45' of the components that are coded by the gli-b1 chromosome locus and the intrinsic quality (gluten viscoelasticity) of cultivars. the proteins from 4 cultivars were subjected to sequential extraction and separated into five groups, respectively, in: nacl, etoh (gliadins-i), etoh + mercaptoethanol (me) (gliadins-ii), acoh + me (glutenins ... | 2016 | 3120804 |
| polymorphism and inheritance of gliadin components controlled by chromosome 6a of spring durum wheat. | the gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (al-lactate, ph 3.1) and two-dimensional (first dimension, al-lactate, ph 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. analysis of hybrids has shown that all components of the alpha zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6a. fourteen variants of gliadin blocks ... | 1988 | 3242490 |
| purification and characterization of variant alcohol dehydrogenase isozymes from durum wheat. | three alcohol dehydrogenase (adh) isozymes from embryos of the durum wheat cultivar bijaga yellow having the variant adh-alb allele were purified using (nh4)2so4 precipitation, gel filtration, and ion-exchange chromatography. adh is a dimeric enzyme. the variant isozyme adh-1-1, which is a homodimer composed of alpha b monomers, was compared with adh-1-5 (homodimer composed of beta a monomers), the product of adh-b1, and the adh-1-3 isozyme (alpha b beta a heterodimer) on a number of parameters ... | 1987 | 3447591 |
| evaluation of lead, cadmium, chromium, copper and zinc by atomic absorption spectroscopy in durum wheat milling products in relation to the percentage of extraction. | 36 samples representative of the 1981 italian durum wheat crop were separated by an industrial italian milling process and the products obtained analyzed by aas for their pb, cd, cr, cu, zn content. the results obtained were: pb ranging from 0.04 to 0.80 ppm, cd from 0.02 to 1.39 ppm, cr from 0.05 to 1.60 ppm, cu from 1.8 to 39.7 ppm and zn from 8.8 to 117.6 ppm. although cr content was relatively homogeneous, pb, cd, cu and zn distribution in the various mill products proved to be rather inhomo ... | 2013 | 3678530 |
| the structure of a high-mr subunit of durum-wheat (triticum durum) gluten. | a high-mr subunit was prepared from durum wheat (triticum durum). viscometric analysis showed that the molecule is rod-shaped, with molecular dimensions of about 50 nm x 1.75 nm (500 a x 17.5 a) in 0.05 m-acetic acid/0.01 m-glycine and 49 nm x 1.79 nm (490 a x 17.9 a) in aq. 50% (v/v) propan-1-ol (+/- 0.01 m-glycine) at 30 degrees c. c.d. spectroscopy in the same solvents indicated the presence of beta-turns, but little alpha-helix [7% in 50% (v/v) propan-1-ol] and no beta-sheet. however, when d ... | 1987 | 3689346 |
| energy minimization for tertiary structure prediction of homologous proteins: alpha 1-purothionin and viscotoxin a3 models from crambin. | homologous proteins may fold into similar three-dimensional structures. spectroscopic evidence suggests this is true for the cereal grain thionins, the mistletoe toxins, and for crambin, three classes of plant proteins. we have combined primary sequence homology and energy minimization to predict the structures alpha 1-purothionin (from durum wheat) and viscotoxin a3 (from viscum album, european mistletoe) from the high resolution (0.945 a) crystal structure of crambin (from crambe abyssinica). ... | 1985 | 3917120 |
| [analysis of whole gliadin from untreated and heat-treated flour with phase inversion high pressure liquid chromatography]. | the gliadins from various wheat flours either untreated or heated (baking, cooking, roller-drying, extrusion-cooking) were extracted with 70% ethanol and analysed by rp-hplc. when flours are heated below 80 degrees c the chromatograms showed no significant change. at temperatures about 80-90 degrees c a peak appeared in all samples (with the exception of durum wheat) at a retention time of 35 min (peak 35) or, if already present, become more pronounced. a further increase in temperature or treat ... | 1985 | 4024746 |
| cytotoxic effects of wheat gliadin-derived peptides. | the peptic-tryptic-cotazym (ptc) digest, obtained from bread wheat gliadin by simulating in vivo protein digestion, was more active than the ptc-digest of durum wheat gliadin in reversibly inhibiting hep-2 cell proliferation and in increasing cellular acid phosphatase. colony-forming ability of the cells was not affected by treatment with both bread or durum wheat gliadin peptides. the peptic-tryptic (pt) digest of bread wheat gliadin also showed agglutinating activity of hep-2 cells. | 1985 | 4071551 |
| [the effect of several chemical mutagens on chromosomal transformations and growth processes in durum wheat]. | 2013 | 4467364 | |
| [control of the adulteration of durum wheat macaroni by the immunodiffusion method]. | 2016 | 4991715 | |
| survival and chlorophyll mutation in triticum durum plants raised from aged seeds. | 1972 | 5059252 | |
| sequential development of meristems in the embryo of triticum durum. a dna autoradiographic and cytophotometric analysis. | 1969 | 5355606 | |
| interaction of genes for pathogenicity in puccinia graminis f. sp. tritici and for resistance in a selection of triticum durum 'acme'. | 1969 | 5377751 | |
| [determination of sitosterol palmitate in different varieties of triticum aestivum and triticum durum]. | 1969 | 5398532 | |
| durum-type wheat with high bread-making quality. | a durum-type wheat (2n = 4x = 28) with high bread-making quality was produced by crossing a durum-wheat variety with a common bread-wheat variety (2n = 6x = 42), backcrossing to the bread-wheat variety for three generations, and then selecting for 28-chromosome plants. the high quality is tentatively attributed to a translocation involving one of the d-genome chromosomes. | 1968 | 5634916 |
| geotropic response of wheat coleoptiles in absence of amyloplast starch. | young coleoptiles of wheat (triticum durum var. henry), depleted of amyloplast starch by incubation at 30 degrees c with gibberellin plus kinetin, retained their geotropic responsiveness. depleted coleoptiles curved upward more slowly than controls, but this was commensurate with their slower growth. the ratio of curvature to growth was about 50 degrees per mm of elongation in both cases. newly excised coleoptiles, though containing much more starch than incubated controls, curved only about 25 ... | 1966 | 5961354 |
| radiation response of dry seeds in two varieties of triticum durum. | 1966 | 5972695 | |
| hypersensitivity to inhaled flour allergens. comparison between cereals. | radioallergosorbent testing (rast) of sera from subjects sensitized to wheat and rye flour indicated that there is significant reaction with seed extracts of 12 cereals (wheat, durum wheat, triticale, cereal rye, barley, rye grass, oats, canary grass, rice, maize, sorghum and johnson grass). results were evaluated in terms of taxonomic relationships and of the electrophoretically determined protein composition of the cereal extracts. rast uptakes were uniformly low in sera from four rhinitic bak ... | 1980 | 6154431 |
| agglutinating activity of gliadin-derived peptides from bread wheat: implications for coeliac disease pathogenesis. | the pt-digest of bread wheat gliadin was very active in agglutinating undifferentiated human k562(s) cells. this activity was quantitatively, but not qualitatively, similar to that of con a or wga. moreover, con a-induced cell agglutination was inhibited by mannan and mannose, wga-induced agglutination by nag only, and cell agglutination induced by bread wheat gliadin peptides was inhibited by each of these three saccharides. not only was mannan the most active saccharide in preventing cell aggl ... | 1984 | 6547342 |
| morphological changes in strains of aspergillus flavus link ex fries and aspergillus parasiticus speare related with aflatoxin production. | two strains of aspergillus flavus link ex fr. and two strains of a. parasiticus speare were cultured on crushed moist wheat (triticum durum var. pané no. 257) for aflatoxin production studies in correlation with morphological changes. the toxicogenic strains were adapted to the substratum by means of successive transfers at regular intervals (72 h.) the amount aflatoxins synthesized by the toxicogenic strains decreased gradually after succesive subculturing. the decrease was accompanied by marke ... | 1980 | 6780911 |
| pulmonary reaction to durum wheat; a constituent of grain dust. | to identify constituents of grain dust responsible for grain handlers' respiratory symptoms, 11 volunteer grain elevator workers underwent inhalation provocation tests with extracts of durum wheat, durum wheat airborne dust, and grain insects and mites. factors that might influence the host response to the challenge were assessed. five of the 11 subjects showed a greater than 20 percent decrement in fev1 after inhalation of durum wheat extracts (airways reaction). the bronchial reactions were im ... | 1982 | 6797794 |
| effects of gliadin-derived peptides from bread and durum wheats on in vitro cultures of human cell lines. implications for coeliac disease pathogenesis. | a peptic-tryptic-cotazym digest, obtained from bread (hexaploid) wheat gliadins under experimental conditions mimicking in vivo protein digestion, was found to reduce in vitro viability of human embryo (mrc-5) and tumor cell (hep-2) lines. time of onset and extent of cytotoxic effects were largely dependent on initial peptide concentrations in the culture medium. the presence of 2% fetal calf serum was capable of delaying, but not of preventing, the onset of cytotoxic effects only in mrc-5 cultu ... | 1983 | 6857727 |
| in vitro assay for predicting protein efficiency ratio as measured by rat bioassay: collaborative study. | seven laboratories collaborated in testing the calculated protein efficiency ratio (c-per and dc-per). the collaborative study required each laboratory to analyze 6 foods and a control protein (anrc casein) for in vitro apparent protein digestibility, amino acid composition, and per via rat bioassay. the 6 foods or food ingredients tested were nonfat dry milk, cooked chicken muscle, protein-fortified dry breakfast cereal, textured soy protein, oat-based dry breakfast cereal, and durum wheat flou ... | 1982 | 7118785 |
| effects of gliadin-derived peptides from bread and durum wheats on small intestine cultures from rat fetus and coeliac children. | peptic-tryptic-cotazym (ptc) digests were obtained, simulating in vivo protein digestion, from albumin, globulin, gliadin and glutenin preparations from hexaploid (bread) wheat as well as from diploid (monococcum) and tetraploid (durum) wheat gliadins. the digest from bread wheat gliadins reversibly inhibited in vitro development and morphogenesis of small intestine from 17-day-old rat fetuses, whereas all the other digests (obtained both from nongliadin fractions and from gliadins from other wh ... | 1982 | 7155669 |
| in vitro proliferation of lymphocytes from celiac children and their first-degree relatives in response to wheat gliadin-derived peptides. | evidence is accumulating which indicates that immune reactions to gliadins are involved in the pathogenesis of celiac disease, as humoral- and cell-mediated immune responses to gliadins have been demonstrated in the jejunal mucosa and the peripheral blood of patients. an abnormal specific immunological response to gliadins is demonstrated not only in celiac children but also in their first-degree relatives. seventy-eight percent of celiac patients, and 67, 87, and 100% of their fathers, siblings ... | 1982 | 7186066 |
| effects of protectants on the activity of wheat mitochondria. | a mitochondrial fraction was isolated from durum wheat seedlings via differential centrifugation using three different tissue grinding buffers. one buffer contained bovine serum albumin (bsa), a protein proven effective in protecting mitochondria from damage during isolation. the other two buffers contained either polyethyleneglycol of m.w. 20,000d (peg-20), a phenolic binding compound or peg-20 plus xad-2, a non-ionic adsorbent material. organelles isolated with the bsa exhibited an average adp ... | 1981 | 7220493 |
| relationship between durum wheat protein properties and pasta dough rheology and spaghetti cooking quality. | 2010 | 7462516 | |
| triticum aestivum puroindolines, two basic cystine-rich seed proteins: cdna sequence analysis and developmental gene expression. | from a mid-maturation seed cdna library we have isolated cdna clones encoding two triticum aestivum puroindolines. puroindoline-a and puroindoline-b, which are 55% similar, are basic, cystine-rich and tryptophan-rich proteins. puroindolines are synthesized as preproproteins which include n- and c-terminal propeptides which could be involved in their vacuolar localization. the mature proteins have a molecular mass of 13 kda and a calculated isoelectric point greater than 10. a notable feature of ... | 1994 | 7516201 |
| characterization of the triticum durum desf. chloroform-methanol-soluble protein family. | the cm (chloroform-methanol-soluble) proteins are low-molecular-weight cysteine-rich proteins that are found in wheat and barley endosperms. a cdna clone encoding a triticum durum (t. durum) cm3 protein has been isolated from a mid-maturation seed cdna library. the t. durum cm3 protein is synthesized as a precursor including a signal peptide (sp) of 25 residues. northern blot analysis shows that in developing seed the highest level of cm3 protein mrna is detected at mid-maturation. the hybridiza ... | 1995 | 7612926 |
| the influence of food structure on postprandial metabolism in patients with non-insulin-dependent diabetes mellitus. | two natural-food mixed meals composed in accordance with the present dietary recommendations were given to 10 diabetic patients on two occasions. the meals were planned to achieve large differences in glycemic index (gi) by altering the food structure but maintaining an identical nutrient composition. in the first study, two meals were compared that contained either pasta or bread made from durum wheat flour. in the second experiment a meal with parboiled rice, red kidney beans, and bread made f ... | 1995 | 7702028 |
| development of a new series of agricultural/food reference materials for analytical quality control of elemental determinations. | ten new agricultural/food reference materials--bovine muscle powder (national institute of standards and technology [nist], code nist rm 8414), whole egg powder (nist rm 8415), microcrystalline cellulose (nist rm 8416), wheat gluten (nist rm 8418), corn starch (nist rm 8432), corn bran (nist rm 8433), whole milk powder (nist rm 8435), durum wheat flour (nist rm 8436), hard red spring wheat flour (nist rm 8437) and soft winter wheat flour (nist rm 8438)--were prepared by application of milling, i ... | 1994 | 7819768 |
| agglutinating activity of wheat gliadin peptide fractions in coeliac disease. | the k 562 (s) cell agglutinating activity of peptides obtained from in vitro digestion of bread wheat gliadins has been shown to be associated with a small fraction (coded as fraction c), that can be easily separated by affinity chromatography of the whole digest on a sepharose 6-b-mannan or sepharose 6-b-oligomers of n-acetyl-glucosamine. although the whole gliadin digests from 12 durum wheat varieties were unable to agglutinate k 562 (s) cells, all these digests were found to contain an active ... | 1995 | 7863509 |
| molecular variation in chloroplast dna regions in ancestral species of wheat. | restriction map variation in two 5-6-kb chloroplast dna regions of five diploid aegilops species in the section sitopsis and two wild tetraploid wheats, triticum dicoccoides and triticum araraticum, was investigated with a battery of four-cutter restriction enzymes. a single accession each of triticum durum, triticum timopheevi and triticum aestivum was included as a reference. more than 250 restriction sites were scored, of which only seven sites were found polymorphic in aegilops speltoides. n ... | 1994 | 7916310 |
| lead and cadmium in some cereal products on the finnish market 1990-91. | lead and cadmium contents were determined in representatively collected (commercial mills, wholesalers) samples of rye flour, breakfast cereals, porridge flakes, muesli cereals and pasta products. the samples were digested by heating them overnight in concentrated hno3. lead and cadmium concentrations were determined by gfaas using a platform and (nh4)h2po4 as a matrix modifier. arc/cl coded wheat flour and other reference materials (nbs 1567a, bcr no. 189, bcr no. 191) were employed for the ana ... | 0 | 8314400 |
| storage changes in the quality of sound and sprouted flour. | sound and sprouted flours (24 and 48 hr) from bread wheat (wl-1562), durum wheat (pbw-34) and triticale (tl-1210) were stored at room temperature (34.8 degrees c) and relative humidity (66.7%) for 0, 45, 90 and 135 days to assess the changes in physico-chemical and baking properties. protein, gluten, sedimentation value, starch and crude fat decreased during storage in all the samples; however, the decrease was more in sprouted flours. free amino acids, proteolytic activity, diastatic activity a ... | 1993 | 8332585 |
| application of the random amplified polymorphic dna technique for the detection of polymorphism among wild and cultivated tetraploid wheats. | development of a high-density genetic linkage map of cultivated wheats using conventional molecular markers has lagged behind the other major food crops such as rice and tomato because of the large genome size and limited levels of genetic polymorphisms. recently, random amplified polymorphic dnas (rapds) have been suggested to provide an alternative to visualize more polymorphism. for the construction of a genetic linkage map in tetraploid wheats, one can use a strategy of intersubspecific cros ... | 1993 | 8349131 |
| single-seed pcr of lmw glutenin genes to distinguish between durum wheat cultivars with good and poor technological properties. | polymerase chain reaction (pcr) was used to amplify low-molecular-weight (lmw) glutenin sequences from genomic dna extracted from a single germinating seed of several durum wheat genotypes. electrophoretic analysis of pcr reactions showed the presence of amplified products characteristic of durum wheat cultivars with good and poor technological properties. this pcr-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting durum wheat genotypes with ... | 1993 | 8400133 |
| purification and activity of a wheat 9-kda lipid transfer protein expressed in escherichia coli as a fusion with the maltose binding protein. | the cdna encoding a wheat (triticum durum) lipid transfer protein of 9 kda was inserted into an escherichia coli expression vector, pih902, and expressed in the bacteria as a fusion with the maltose binding protein. the fusion protein was then purified to homogeneity and subjected to factor xa cleavage. although complete cleavage of the fusion protein was obtained, the expected lipid transfer protein was not recovered; it appears to be degraded during protease digestion. however, a fluorescent l ... | 1995 | 8535151 |
| [rapd markers for wheat chromosomes in langdon disomic substitution lines]. | optimal conditions were developed for a random amplified polymorphio dna (rapd) assay of hexaploid bread wheat and tetraploid durum wheat. amplitaq stoffcl fragment was found to be better than taq dna polymerase in generating rapds. studies on chromosome specific rapd markers of the a- and b- and d-genome were performed using the complete set of langdon disomic substitution lines and the parental lines (langdon and chinese spring) as templete. seven out of twelve arbitrary primers (all operon 10 ... | 1996 | 8695176 |
| stimulation of atp synthesis via oxidative phosphorylation in wheat mitochondria irradiated with helium-neon laser. | in order to ascertain whether oxidative phosphorylation in plant mitochondria is sensitive to light, coupled durum wheat (triticum durum desf.) mitochondria were irradiated with a low power continuous wave helium-neon laser (fluence: 2 joules/cm2), with measurements made of certain processes related to atp production. as a result of irradiation, an increase in the rate of atp synthesis was found, as continuously monitored via luciferine/luciferase, moreover the mitochondrial atp and adp endogeno ... | 1996 | 8799337 |
| potential of two-dimensional electrophoresis in routine identification of closely related durum wheat lines. | four closely related durum wheat varieties were compared by computer-assisted analysis of two-dimensional electrophoretic maps of leaf proteins. a low inter-varietal polymorphism was revealed and seven reliable qualitatively varying proteins allowed rapid visual identification of genotypes. for numerous spots, presence/absence or quantitative variations were greatly affected by a batch effect. several criteria that should be used to discard unreliable spots or gels a priori were reviewed. nevert ... | 1997 | 9059841 |
| a small peptide from durum wheat gliadin prevents cell agglutination induced by prolamin-peptides toxic in coeliac disease. | a peptide (m.w. 1157.5 da) able to prevent the agglutination of k562(s) cells induced by the peptic-tryptic prolamine digests of the cereals toxic in coeliac disease (i.e. bread wheat, rye, barley and oat) was characterized as one of the components of the peptic-tryptic digest of durum wheat gliadin. this peptide was synthesized in a high degree of purity with the solid phase method with the applied biosystem 431a. an amino acid sequence was identified in the 1157.5 da peptide as being related t ... | 0 | 9217307 |
| nutritional evaluation of a high-temperature dried soft wheat pasta supplemented with cowpea (vigna unguiculata (l) walp). | the objective of this study was to determine the nutritional quality of the pasta described above. the work was unique in the following aspects: 1) the drying process was a nonconventional method, consisting of a high-temperature short-time (htst) process; 2) the nutritional quality of the wheat pasta was improved by the cowpea supplementation. cowpea was especially chosen due to it being a staple food in the diet of the people in northeast brazil. benefits attributed to the htst drying process ... | 1996 | 9239294 |
| partial characterization of glutathione s-transferases from wheat (triticum spp.) and purification of a safener-induced glutathione s-transferase from triticum tauschii. | hexaploid wheat (triticum aestivum l.) has very low constitutive glutathione s-transferase (gst) activity when assayed with the chloroacetamide herbicide dimethenamid as a substrate, which may account for its low tolerance to dimethenamid in the field. treatment of seeds with the herbicide safener fluxofenim increased the total gst activity extracted from t. aestivum shoots 9-fold when assayed with dimethenamid as a substrate, but had no effect on glutathione levels. total gst activity in crude ... | 1997 | 9276955 |
| characterization of wheat thioredoxin h cdna and production of an active triticum aestivum protein in escherichia coli. | two cdna clones, ptam13.38 and ptd14.13.2, encoding a triticum aestivum and a triticum durum thioredoxin h, respectively, were isolated from mid-maturation seed cdna libraries. the t aestivum thioredoxin h has a molecular mass of 13.5 kda and that from t durum has a molecular mass of 13.8 kda. these two wheat thioredoxin h are 98.5% similar and contain the canonical wcgpc active site and the important structural and functional amino acids that are conserved in thioredoxin sequences. the recombin ... | 1998 | 9523703 |
| characterization of cadmium binding, uptake, and translocation in intact seedlings of bread and durum wheat cultivars | high cd content in durum wheat (triticum turgidum l. var durum) grain grown in the united states and canada presents potential health and economic problems for consumers and growers. in an effort to understand the biological processes that result in excess cd accumulation, root cd uptake and xylem translocation to shoots in seedlings of bread wheat (triticum aestivum l.) and durum wheat cultivars were studied. whole-plant cd accumulation was somewhat greater in the bread wheat cultivar, but this ... | 1998 | 9536059 |
| [quality of pastas supplemented with rice bran]. | the objective of this research was to investigate the potential of using rice bran as an ingredient in pastas spaghetti type. two of the pastas were made with semolina from durum as raw material, supplemented with 10 and 20% rice bran. the other two were made with granular flour and the same percentage of rice bran. proximate composition of raw material was analyzed. pastas were elaborated in a local industry. composition, proximal, color, texture, and sensorial quality of pastas were determined ... | 1997 | 9659429 |
| [development of a soup pasta based on the taste and nutritional requirements for aged people]. | the objective of this project was to elaborate a soup paste product based on cereal/leguminous mixture by cold extrusion method according to the preferences and limitations of the senescent population. formulas were obtained by a computation food mixtures program; the limitations applied were: protein quantity and quality. selected mixtures has to contain protein values over 13.2 g/100 g and a chemical assessment of leucine, lysine and methionine-cistein not lower than 75% of the fao/oms 1973 re ... | 1997 | 9659431 |
| structural specificities and significance for coeliac disease of wheat gliadin peptides able to agglutinate or to prevent agglutination of k562(s) cells. | two peptides corresponding to bread wheat a-gliadin fragments 31-43 and 44-55, well known for their ability to damage the coeliac disease intestinal mucosa both in vitro and in vivo, have been confirmed to be very active in inducing in vitro agglutination of k 562 (s) cells. removal of six amino acid residues from the carboxy-terminal end of the 31-43 peptide, or of five amino acid residues from the amino terminal end of the 44-55 peptide, resulted in a lower, but still very significant, cell ag ... | 1998 | 9699797 |
| characterization of zinc uptake, binding, and translocation in intact seedlings of bread and durum wheat cultivars | durum wheat (triticum turgidum l. var durum) cultivars exhibit lower zn efficiency than comparable bread wheat (triticum aestivum l.) cultivars. to understand the physiological mechanism(s) that confers zn efficiency, this study used 65zn to investigate ionic zn2+ root uptake, binding, and translocation to shoots in seedlings of bread and durum wheat cultivars. time-dependent zn2+ accumulation during 90 min was greater in roots of the bread wheat cultivar. zn2+ cell wall binding was not differen ... | 1998 | 9733541 |
| colonization of wheat roots by an exopolysaccharide-producing pantoea agglomerans strain and its effect on rhizosphere soil aggregation | the effect of bacterial secretion of an exopolysaccharide (eps) on rhizosphere soil physical properties was investigated by inoculating strain nas206, which was isolated from the rhizosphere of wheat (triticum durum l.) growing in a moroccan vertisol and was identified as pantoea aglomerans. phenotypic identification of this strain with the biotype-100 system was confirmed by amplified ribosomal dna restriction analysis. after inoculation of wheat seedlings with strain nas206, colonization incre ... | 1998 | 9758793 |
| improved method for the determination of furosine in food by capillary electrophoresis. | a previously described method for determination of furosine by capillary zone electrophoresis in food has been improved for greater accuracy and sensitivity. acid-hydrolyzed samples are subjected to solid phase extraction; then they are dried and redissolved before injection. the electrophoresis separation is performed in a fused silica capillary tube (50 microns inside diameter) with an extended-path-length detection cell using a 3-(n-morpholino)-2-hydroxypropanesulfonic acid solution at ph 7.0 ... | 1998 | 9798165 |
| antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought | we analyzed antioxidative defenses, photosynthesis, and pigments (especially xanthophyll-cycle components) in two wheat (triticum durum desf.) cultivars, adamello and ofanto, during dehydration and rehydration to determine the difference in their sensitivities to drought and to elucidate the role of different protective mechanisms against oxidative stress. drought caused a more pronounced inhibition in growth and photosynthetic rates in the more sensitive cv adamello compared with the relatively ... | 1999 | 10069848 |
| production in escherichia coli and site-directed mutagenesis of a 9-kda nonspecific lipid transfer protein from wheat. | the sequence encoding a wheat (triticum durum) nonspecific lipid transfer protein of 9 kda (nsltp1) was inserted into an escherichia coli expression vector, pet3b. the recombinant protein that was expressed accumulated in insoluble cytoplasmic inclusion bodies and was purified and refolded from them. in comparison with the corresponding protein isolated from wheat kernel, the refolded recombinant protein exhibits a methionine extension at its n-terminus but has the same structure and activity as ... | 1999 | 10103017 |
| the existence of the k(+) channel in plant mitochondria. | in this study, evidence is given that a number of isolated coupled plant mitochondria (from durum wheat, bread wheat, spelt, rye, barley, potato, and spinach) can take up externally added k(+) ions. this was observed by following mitochondrial swelling in isotonic kcl solutions and was confirmed by a novel method in which the membrane potential decrease due to externally added k(+) is measured fluorimetrically by using safranine. a detailed investigation of k(+) uptake by durum wheat mitochondri ... | 1999 | 10480870 |
| relationship between fusarium graminearum and alternaria alternata contamination and deoxynivalenol occurrence on argentinian durum wheat. | a mycological survey was carried out on durum wheat (triticum durum) samples from the main production area of argentina. the isolation frequency and relative density of species of dematiaceous fungi, and genus fusarium were calculated. alternaria alternata and fusarium graminearum were the predominant fungal species. an analysis of deoxynivalenol (don) natural contamination was also performed on a limited number of samples (60). don contamination levels in positive samples ranged from 26 to 6400 ... | 1998 | 10481289 |
| capillary electrophoresis of peptides and proteins in acidic, isoelectric buffers: recent developments. | the use of isoelectric buffers in capillary zone electrophoresis is here reviewed. such buffers allow delivery of very high voltage gradients (up to 1000 v/cm in relatively large bore capillaries, e.g. 75-100 microm i.d.), permitting separations of the order of a few minutes and thus conserving (in fact favouring) very high resolution due to minimal, diffusion-driven, peak spreading. isoelectric asp (pi 2.77 at 50 mm concentration and 25 degrees c) provides a medium of high resolving power for g ... | 1999 | 10481947 |
| a family of dispersed repeats in the genome of vicia faba: structure, chromosomal organization, redundancy modulation, and evolution. | a family of repeated dna sequences of about 1200 bp in length and bordered by well-conserved, 18 bp inverted repeats (vfb family) was found in the nuclear genome of vicia faba. the structure, chromosomal organization, redundancy modulation and evolution of these sequences were investigated. they are enriched in a+t base pairs (about 40% g+c) and lack any obvious internally repeated motif. a 64%-73% nucleotide sequence identity was found when pairwise comparisons between vfb sequences were carrie ... | 1999 | 10525968 |
| variation in the degree of d-xylose substitution in water-extractable european durum wheat (triticum durum desf.) semolina arabinoxylans. | durum wheat (triticum durum desf.) semolina water-extractable arabinoxylan (tweax) (yield 0.28%, arabinose-to-xylose ratio (a/x) 0. 62) was fractionated by a stepwise increase in ethanol concentration (up to 65%). the a/x ratios of the resulting fractions varied between 0.42 and 0.80. with increasing ethanol concentrations, increasing a/x ratios went hand in hand with a relative increase of low molecular weight compounds, indicating that high molecular weight compounds with a low a/x ratio are p ... | 1999 | 10552456 |
| purification and characterization of a lipoxygenase enzyme from durum wheat semolina. | purification of a lipoxygenase enzyme from the cultivar tresor of durum wheat semolina (triticum turgidum var. durum desf) was reinvestigated furnishing a new procedure. the 895-fold purified homogeneous enzyme showed a monomeric structure with a molecular mass of 95 +/- 5 kda. among the substrates tested, linoleic acid showed the highest k(cat)/k(m) value; a beta-carotene bleaching activity was also detected. the enzyme optimal activity was at ph 6. 8 on linoleic acid as substrate and at ph 5.2 ... | 1999 | 10552472 |
| identification of wheat and tritordeum chromosomes by genomic in situ hybridization using total hordeum chilense dna as probe | total genomic hordeum chilense dna probe was hybridized to somatic chromosome spreads of triticum aestivum 'chinese spring' and to four advanced tritordeum lines, the latter being the fertile amphiploid between h. chilense and durum wheat (2n = 6x = 42, aabbh(ch)h(ch)). the probe hybridized strongly to the b-genome chromosomes and to one or two bands on the a-genome chromosomes present in both wheat and tritordeum alloploids. bands on chromosomes 1d, 2d, and 7d from hexaploid wheat were also det ... | 1999 | 10659787 |
| superoxide and hydroxyl radical generation, and superoxide dismutase in psii membrane fragments from wheat. | illumination of photosystem ii (psii) membrane fragments of wheat (triticum durum desf. cv. adamello) gave rise to both o2*- and *oh radicals adducts of the novel spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-n-oxide (depmpo). with time, *oh became predominant displaying the conversion of o2*- into *oh. an intrinsic activity of superoxide dismutase (sod) was found in psii. photoreduction of nitroblue tetrazolium (nbt) by psii membrane fragments was induced by the addition of sodium azide ... | 1999 | 10694034 |
| activated oxygen generation from thylakoids: a novel spin trap. | using a novel phosphorylated spin trap, 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-n-oxide (depmpo), an analogue of the commonly used spin trap 5,5'-dimethyl-1-pyrroline n-oxide (dmpo), we have investigated the production of oxygen radical species under illumination of thylakoids from wheat (triticum durum desf cv. ofanto). depmpo reacted with superoxide (o2*-) and hydroxyl radical (ho*) forming distinctive spin trap adducts. spectra of (o2*-) and ho* adducts of depmpo were recorded in the pres ... | 1999 | 10694060 |
| effects of fatty acids, nucleotides and reactive oxygen species on durum wheat mitochondria. | linoleic acid (la) and other fatty acids added to respiring durum wheat mitochondria (dwm) were found to cause a remarkable membrane potential (deltapsi) decrease, as monitored by measuring safranin fluorescence. the rate of deltapsi decrease showed (i) saturation dependence on la concentration; (ii) fatty acid specificity; (iii) inhibition by externally added atp, gdp, gtp and mg(2+) and (iv) sigmoid dependence upon initial deltapsi, thus suggesting the existence of an active plant mitochondria ... | 2000 | 10722851 |
| bioaccumulation of cadmium from durum wheat diets in the livers and kidneys of mice. | 2000 | 10754049 | |
| induction of apoptosis in caco-2 cells by wheat gliadin peptides. | recent experimental evidence suggests that enterocyte apoptosis is greater than hitherto assumed and may be responsible for villous atrophy in coeliac disease. we have previously demonstrated that a small peptide (m.w. 1157.5 da), identified as the sequence h(2)n-gln-gln-pro-gln-asp-ala-val-gln-pro-phe-cooh from durum wheat gliadin, is able to prevent k 562 (s) cell agglutination induced by the peptic-tryptic digests (pt) of prolamin fractions from the cereals which are not tolerated in coeliac ... | 2000 | 10771132 |
| value assignment of nutrient concentrations in five standard reference materials and six reference materials. | a number of food-matrix reference materials (rms) are available from the national institute of standards and technology (nist) and from agriculture canada through nist. most of these materials were originally value-assigned for their elemental composition (major, minor, and trace elements), but no additional nutritional information was provided. two of the materials were certified for selected organic constituents. ten of these materials (standard reference material [srm] 1,563 cholesterol and f ... | 2017 | 10772181 |
| [in vitro androgenesis response of durum wheat disomic substitution]. | the variety 'langdon' and its substitution series were used to evaluate the effect of each substituted chromosome of the a and b genomes on the in vitro androgenetic potential. this study showed the implication of chromosomes 1b and 5b in repressing embryogenesis. genes located on these chromosomes seem to have an inhibitor effect. the substitution of these chromosomes by their homeologous ones from the d genome increased the number of embryos while with the presence of the original genes the nu ... | 2000 | 10782328 |
| trace metals in soft and durum wheat from italy. | a survey was carried out with the aim to assess the levels of some toxic (cadmium, lead) and essential (copper, zinc) trace metals in wheat grown in italy. a total of 178 samples of soft wheat grain and 239 samples of durum wheat grain from all the italian wheat-growing regions were pooled into 35 and 38 representative samples respectively. after dry ashing, cadmium and lead were determined by graphite furnace atomic absorption spectrometry (gfaas), whereas copper and zinc were determined by fla ... | 2000 | 10793853 |
| qualitative and quantitative analysis of wheat gluten proteins by liquid chromatography and electrospray mass spectrometry. | based on analysis by liquid chromatography/electrospray ionisation mass spectrometry, we have developed a new method for fast and sensitive fingerprinting of gliadins and glutenins in wheat flour. using this procedure the two protein fractions from seven durum wheat varieties have been analysed by high resolution high performance liquid chromatographic separation coupled to accurate determination of molecular mass. in this way, the molecular mass of the single components from both gliadin and gl ... | 2000 | 10825254 |
| [the inheritance of the traits of resistance to fungal diseases by distant hybrids of wheat with amphiploids]. | the inheritance of resistance to powdery mildew, leaf rust and septorios of hybrids from crosses of wheat--rye and two wheat--elymus amphiploids with durum and bread wheat was studied. spontaneous hybridization was revealed to be a determining factor of the new available trait formation in the wide crosses. control of the leaf rust and heary leaf traits by the same alien chromosome of elytricum fertile is confirmed. while in the wheat--aegilops amphiploid h74/90-258 these traits are not linked a ... | 2015 | 10857202 |
| endoxylanases in durum wheat semolina processing: solubilization of arabinoxylans, action of endogenous inhibitors, and effects on rheological properties. | endoxylanases seriously affect the rheological properties of durum wheat (triticum durum desf.) semolina spaghetti doughs prepared with, and as evaluated, by the farinograph. under the experimental conditions, control doughs (34.9% moisture content) made from two semolinas (sema and semb) yielded a maximal consistency of 525 and 517 farinograph units (fu), with, respectively, 19.4 and 16.4% of the total level of arabinoxylans (tot-ax) being water-extractable (we-ax). when 75.4 somogyi units/50 g ... | 2000 | 10888491 |
| front-surface absorbance spectra of wheat flour: determination of carotenoids. | front-surface absorbance spectra of wheat flours in the 250-650 nm region can be obtained by measuring reflectance spectra with a conventional spectrofluorometer suitably set to detect light scattered from powder samples. the spectra recorded on flour samples, obtained from seeds of four bread and five durum wheats, show high-intensity absorption bands due to aromatic amino acids of wheat proteins and low-intensity bands due to chromophores bound to low-molecular-weight compounds. the intensity ... | 2000 | 10888525 |
| the cold dependent accumulation of cor tmc-ap3 in cereals with contrasting, frost tolerance is regulated by different mrna expression and protein turnover. | the accumulation of specific cold-regulated (cor) proteins is a component of the hardening process and different amount of cor proteins has been related to different degrees of cold tolerance. a number of different mechanisms controls the accumulation of the cor proteins in the plant cells. in this work we describe the mechanisms controlling the accumulation of the cor protein tmc-ap3, a putative chloroplastic amino acid selective channel protein [1] in barley, durum, wheat, emmer and bread whea ... | 0 | 10908804 |
| two-dimensional electrophoresis of soluble leaf proteins, isolated from two wheat species (triticum durum and triticum aestivum) differing in sensitivity towards nacl. | plants of two wheat species (triticum aestivum cv. tanit and t. durum cv. ben bachir), differing in their sensitivity to nacl were cultivated in the presence or absence of 100 mm nacl for 21 days. soluble proteins extracted from leaves were analyzed by two-dimensional electrophoresis in order to detect nacl-induced changes in the polypeptide patterns. in all, 500 spots were detected. results showed species-dependent differences. the greatest alterations in the polypeptide profiles following salt ... | 2000 | 10939462 |
| analysis by two-dimensional electrophoresis of the effect of salt stress on the polypeptide patterns in roots of a salt-tolerant and a salt-sensitive cultivar of wheat. | the effect of salt stress on the polypeptide levels in roots of two wheat (triticum durum) cultivars with different sensitivity to nacl (cv. ben bachir, sensitive; cv. chili, tolerant), was examined by two-dimensional polyacrylamide gel electrophoresis. blue-stained gels were analyzed by visual inspection to identify changes that resulted when seedlings were grown in the presence of 200 mm nacl for four days. although the protein patterns for control and salt-stressed seedlings were qualitativel ... | 2000 | 10939473 |
| isolation of a cdna encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat. | screening of a wheat (triticum aestivum) cdna library for starch-branching enzyme i (sbei) genes combined with 5'-rapid amplification of cdna ends resulted in isolation of a 4,563-bp composite cdna, sbe1c. based on sequence alignment to characterized sbei cdna clones isolated from plants, the sbeic predicted from the cdna sequence was produced with a transit peptide directing the polypeptide into plastids. furthermore, the predicted mature form of sbeic was much larger (152 kd) than previously c ... | 2000 | 10982440 |
| ph1 gene derived from aegilops speltoides induces homoeologous chromosome pairing in wide crosses of triticum aestivum. | the present study was conducted to investigate the effectiveness of the phi gene transferred from aegilops speltoides into bread wheat cultivar chinese spring (cs) in inducing homoeologous chromosome pairing in interspecific crosses using the chinese spring line, cs(phi), carrying the gene. chinese spring, as well as cs(phi), were crossed as female parents with three accessions of ae. kotschyi (uuss), one accession of secale cereale (rr), two amphiploids of triticum durum-ae. caudata (aabbcc), a ... | 2006 | 10994715 |
| fractionation and reconstitution experiments provide insight into the role of gluten and starch interactions in pasta quality. | commercial durum wheat (triticum durum desf.) semolina was fractionated into starch, gluten, and water extractables. starch surface proteins and surface lipids were removed, and two starches with manipulated granule size distributions were produced to influence starch properties, affecting its interaction with other semolina components. reconstituted spaghetti was made with untreated (control) or treated starches. the pasta made from the starting semolina material had lower cooking time and was ... | 2000 | 10995268 |