Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the reaction of mitochondria in the coleoptiles of rice (oryza sativa l.) with diaminobenzidine. | diaminobenzidine, dab, was applied to segments of aerobically and anaerobically grown coleoptiles of rice, oryza sativa l., with the object of studying the location of cytochrome oxidase at the electron-microscope level. a specific staining of mitochondrial cristae and inner membrane was obtained, with no reaction in other organelles; with extended periods of incubation, the reaction product filled the mitochondria completely. in anaerobically grown coleoptiles, the reaction was much slower and ... | 1993 | 46231 |
| penicillin-induced formation of ribonuclease in rice (oryza sativa l.) endosperm and its inhibition by abscisic acid. | penicillin stimulates the formation of ribonuclease in embryoless rice (oryza sativa l.) endosperm and enhances gibberelin-induced response. penicillin-induced rnase production is completely inhibited by abscisic acid. | 1979 | 227713 |
| protein quality in cereals and pulses. 1. application of microbiological and other in vitro methods in the evaluation of rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). | 1. the streptococcus zymogenes assay procedure was modified and used in the estimation of available methionine and relative nutritional value in rice (oryza sativa l.), sorghum (sorghum vulgare pers.), barley and field beans (vicia faba l.). the results were assessed in relation to the content of tannins and 'dye-binding lysine', and to published information on the nutritional quality of some of the test samples. 2. in grain of ten varieties of sorghum, for which other workers had reported a cor ... | 1979 | 427087 |
| herbimycin, a new antibiotic produced by a strain of streptomyces. | herbimycin, a new antibiotic, was isolated from the fermentation broth of streptomyces hygroscopicus strain no. am-3672, a soil isolate. the molecular formula of herbimycin was determined to be c30h42n2o9. herbimycin was found to have potent herbicidal activity against most mono- and di-cotyledonous plants, especially against cyperus microiria steud. however, oryza sativa showed strong resistance to herbimycin. | 1979 | 468713 |
| purification and characterization of a trypsin inhibitor from rice bran. | a trypsin inhibitor was isolated and purified from the bran of rice, oryza sativa, by extraction with 1% sodium chloride, heat treatment, ammonium sulfate precipitation, ion-exchange chromatography on a cm-sephadex c-25 and gel filtration on a sephadex g-75. the final preparation was homogeneous by electrophoretic analysis. rice bran trypsin inhibitor (rbti) had a molecular weight of about 14,500 and an isoelectric point of 8.07. the amino acids, acid composition was characterized by high conten ... | 1979 | 501453 |
| [bean and rice mixtures of high protein value (author's transl)]. | the optimum content of different rice (oryza sativa) and bean (vigna unguiculata l.) combinations was studied in albino rats. the mixture containing 3 g of rice proteins, corresponding to 41g of rice "in natura", and 7 g of bean protein, corresponding to 59 g of bean "in natura", presented the highest protein efficiency ratio and the feed efficiency ratio. since the limiting amino acid of this mixture was methionine, new assays using varying levels of this amino acid as a supplement were carried ... | 1979 | 515465 |
| production of 9-beta-d-arabinofuranosyladenine by a new species of streptomyces and its herbicidal activity. | 9-beta-d-arabinofuranosyladenine (ara-a) was produced by a new species of streptomyces designated as s. herbaceus. ara-a was found to have potent herbicidal activity against echinochloa crus-galli, digitaria adscendens and chenopodium ficifolium by the treatment with ara-a before germination of these plants. however, oryza sativa had strong resistance to ara-a. | 1979 | 528365 |
| light-induced development of photosynthetic enzymes in etiolated detached leaves of oryza sativa and setaria italica during greening. | 1977 | 612539 | |
| factors modifying radiation induced stimulation in plants: pre-irradiation seed moisture content. | the effect of moisture content of seeds at the time of irradiation in relation to radiation-induced stimulation was investigated on rice (oryza sativa c.v. d-6-2-2). the optimum moisture content was 8% for stimulation measured as seedling height. it is concluded that seed moisture at the time of irradiation plays an important role in the expression of stimulation and its reproducibility. | 1977 | 866584 |
| mutagenic effect of argemone oil in oryza sativa l. | in view of the large scale adulteration of edible oils with argemone oil, a study was undertaken to test its mutagenicity on rice seed. in treatment with screw-pressed oil a marked reduction in seed germination and high chlorophyll mutation and mutant frequencies were observed. in sequential treatments between gamma rays and argemone oil both mutation and mutant frequencies were significantly higher than that of individual treatments. this study clearly indicates that argemone oil is a potent mu ... | 1977 | 876267 |
| in vitro development of plants from microspores of rice. | rice (oryza sativa l., 2n=24) anthers containing microspores in the early-uninucleate to first-mitosis stages were induced successfully to develop into plants in vitro through an intermediary step of callus formation. callus initiation occurred with highest frequency in anthers containing mid-uninucleate imcrospores. the callus derived from different stages of microspore development differed in the potential to differentiate into plants. the plants regenerated from pollen callus were predominant ... | 1977 | 903172 |
| hydrogen isotope effects in the cyclization of d-glucose 6-phosphate by myo-inositol-1-phosphate synthase. | myo-inositol-1-phosphate synthase (ec 5.5.1.4) from rat testis, acer pseudoplatanus l. cell culture and oryza sativa l. cell culture, converted d-[5-3h]glucose 6-phosphate to myo-[2-3h]inositol 1-phosphate at rates ranging from 0.21 to 0.48 that of unlabeled substrate. d-[3-3h]- and d-[4-3h]glucose 6-phosphate were converted at approximately the same rate as that of unlabeled substrate. in the case of testis enzyme, storage as a frozen solution further lowered the rate with d-[5-3h]glucose 6-pho ... | 1977 | 903359 |
| volatile components of unprocessed rice (oryza sativa l.). | 1976 | 1002944 | |
| quantitative genetically nonequivalent reciprocal crosses in cultivated plants. | quantitative expressions of character difference between reciprocal crosses have been studied by different researchers in a number of plant species, such as epilobium, zea mays, oryza sativa, hordeum sativum, triticum aestivum, trifolium hybridum, linum usitatissimum, nicotiana rustica, and others. in all cases it was found that the nonequivalence of reciprocal crosses manifested itself beginning with the f1 generation, with the exception of some flax crosses in which reciprocals differed beginn ... | 2015 | 1032105 |
| reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. | the intracellular localization of phytochrome was seen in dark-grown oat (avena sativa l., cv. garry) and rice (oryza sativa l., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. phytochrome is generally distributed throughout the cytoplasm in cells of tissue that had not been exposed to light prior to fixation. within, at most, 8 min after the onset of saturating red irradiation, phytochrome, now present in the far-red-absor ... | 1975 | 1093170 |
| the limit dextrinases from ungerminated oats (avena sativa l.) and ungerminated rice (oryza sativa l.). | the limit dextrinases from ungerminated oats and rice have been purified, and their substrate specificity compared with a bacterial isoamylase preparation. both cereal enzymes could hydrolyse (1 yields6)-alpha-d-glucosidic linkages in oligosaccharide alpha-dextrins, pullulan, amylopectin, and the beta-limit dextrins of amylopectin and glycogen. however, under comparable conditions, they were unable to attack glycogens. | 1975 | 1170015 |
| molecular characterization of cdna encoding for adenylate kinase of rice (oryza sativa l.). | two types of genes (adk-a, and adk-b) encoding for adenylate kinase (ak, ec 2.7.4.3.) were isolated from the cdna library constructed from poly(a)+ rna of rice (oryza sativa l.). two cdnas were heterogeneous at 5' and 3' ends of non-coding sequences and had possible polyadenylation signals. one of the genes, adk-a, had 1154 bp sequences encoding 241 amino acid residues, while the other type, adk-b, contained 1085 bp sequences encoding for 243 amino acid residues. homology between adk-a and adk-b ... | 1992 | 1302636 |
| characterization of a plant sine, p-sine1, in rice genomes. | we have previously found that a short interspersed element (sine), named p-sine1, is present in the waxy gene of oryza sativa in two copies. here, we cloned five members of p-sine1 located at other loci in o. sativa and determined their nucleotide sequences. these sequences had a t-rich pyrimidine tract at their defined 3' end and were flanked by direct repeats of a sequence of mostly 14-15 bp long like p-sine1s in the waxy gene. the consensus sequence derived from total seven members of p-sine1 ... | 1992 | 1326298 |
| herbicide-resistant indica rice plants from irri breeding line ir72 after peg-mediated transformation of protoplasts. | the commercially important indica rice cultivar oryza sativa cv. ir72 has been transformed using direct gene transfer to protoplasts. peg-mediated transformation was done with two plasmid constructs containing either a camv 35s promoter/hph chimaeric gene conferring resistance to hygromycin (hg) or a camv 35s promoter/bar chimaeric gene conferring resistance to a commercial herbicide (basta) containing phosphinothricin (ppt). we have obtained so far 92 hgr and 170 pptr ir72 plants from protoplas ... | 1992 | 1333295 |
| characterization and genetic mapping of a short, highly repeated, interspersed dna sequence from rice (oryza sativa l.). | a short, highly repeated, interspersed dna sequence from rice was characterized using a combination of techniques and genetically mapped to rice chromosomes by restriction fragment length polymorphism (rflp) analysis. a consensus sequence (ggc)n, where n varies from 13-16, for the repeated sequence family was deduced from sequence analysis. southern blot analysis, restriction mapping of repeat element-containing genomic clones, and dna sequence analysis indicated that the repeated sequence is in ... | 1992 | 1347144 |
| chromosome location of oryza sativa recombination linkage groups. | in situ hybridization, a powerful tool for the molecular cytogeneticist, can be used to physically map repetitive, low-copy, and unique dna sequences in plant chromosomes. with the availability of a recombination map in oryza sativa l. and an improved in situ hybridization technique, this study was designed to establish the relationship between the genetic and physical distances of the rice restriction fragment length polymorphism map. analysis indicated that considerable variation can exist bet ... | 1992 | 1356266 |
| genetic and physical analysis of the rice bacterial blight disease resistance locus, xa21. | nearly isogenic lines (nils) of rice (oryza sativa) differing at a locus conferring resistance to the pathogen xanthomonas oryzae pv. oryzae were surveyed with 123 dna markers and 985 random primers using restriction fragment length plymorphism (rflp) and random amplified polymorphic dna (rapd) analysis. one chromosome 11 marker (rg103) detected polymorphism between the nils that cosegregated with xa21. all other chromosome 11 dna markers tested were monomorphic between the nils, localizing the ... | 1992 | 1362973 |
| molecular cloning and characterization of gravity specific cdna in rice (oryza sativa l.) suspension callus. | rice (oryza sativa l. var. nipponbare) suspension callus was exposed to gravity stress at 450,000 g for 2 hours, after which poly(a)+rna was isolated and a cdna library was constructed. three different gravity specific cdnas, namely, gsc 128, gsc 233 and gsc 381 of 0.67, 0.60 and 0.68 kilobase pairs and transcripts of 1.9, 1.6 and 2.0 kb, respectively, were isolated by differential screening and northern hybridization. the maximum level of transcript was achieved after 4 hours of exposure to gra ... | 1992 | 1363521 |
| purification and characterization of rice peroxidases. | four peroxidase components, named rp-2, 4, 6, and 7, were isolated from rice (oryza sativa l.) green leaves. isoelectric focusing indicated that each preparation was homogeneous. the molecular weights of rp-2, 4, 6, and 7 estimated by sds-page were 48,000, 48,000, 40,000, and 39,500, and their isoelectric points were 5.4, 8.1, 9.3, and 9.2, respectively. the activity of every preparation was maximum around ph 5.0. antisera against these purified enzymes were raised in rabbits. ouchterlony double ... | 1991 | 1368754 |
| isolation and characterization of cdna clones encoding cdc2 homologues from oryza sativa: a functional homologue and cognate variants. | using probes obtained by pcr amplification, we have isolated two cognate rice cdnas (cdc2os-1 and cdc2os-2) encoding structural homologues of the cdc2+/cdc28 (cdc2) protein kinase from a cdna library prepared from cultured rice cells. comparison of the deduced amino acid sequences of cdc2os-1 and cdc2os-2 showed that they are 83% identical. they are 62% identical to cdc28 of saccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to rice r2, a cdc2-related ... | 1992 | 1376401 |
| nucleotide sequence of a cdna encoding a beta subunit of the mitochondrial atpase from rice (oryza sativa). | 1992 | 1387558 | |
| efficiency of the 5'-terminal sequence (omega) of tobacco mosaic virus rna for the initiation of eukaryotic gene translation in escherichia coli. | recent studies have demonstrated that the 5' leader (omega sequence) of tobacco mosaic virus rna has a certain enhancing capacity for translation of mrna in both prokaryotes and eukaryotes. in order to estimate the efficiency of omega to initiate translation of mrna in escherichia coli, in comparison to the shine-dalgarno (s/d) sequence, we have inserted eight different eukaryotic genes into two types of e. coli expression vectors containing one constitutive promoter (p1) but different translati ... | 1992 | 1396694 |
| the wheat mitochondrial genome contains an orf showing sequence homology to the gene encoding the subunit 6 of the nadh-ubiquinone oxidoreductase. | a region of the mitochondrial (mt) dna of wheat was studied because of its homology with other plant mtdnas. sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. comparison of the sequence of the putative polypeptide with the protein sequence data of the swiss-prot library reveals homology with subunit 6 of the nadh-ubiquinone complex of mitochondria from marchantia polymorpha, podospora anserina, chlamydomonas reinhardtii and of chloroplasts from m. polymo ... | 1992 | 1421143 |
| factors influencing agrobacterium-mediated transient expression of gusa in rice. | transient expression of gus in rice (oryza sativa l.) mediated by agrobacterium tumefaciens was characterized using binary vectors containing gusa genes that express minimal (pkiwi105 and pcnl1) or no (p35s-gus-int and pcnl56) gus activity in bacteria. four-day old seedlings obtained from seeds or immature embryos of rice were cut into shoot, root, and seed remnants and inoculated with various strains of a. tumefaciens. transient gus expression events were quantitated histochemically by determin ... | 1992 | 1463839 |
| isolation of genes abundantly expressed in rice anthers at the microspore stage. | a cdna library of rice (oryza sativa l.) has been constructed from anthers at an early stage of pollen development. by differential screening of the library, we have isolated cdnas of two genes, designated as osc4 and osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. expression of osc4 is absent in mature anthers, while osc6 is present although the expression decays during pollen maturation. a comparison of th ... | 1992 | 1463854 |
| the amino acid sequence of a 20 kda bifunctional subtilisin/alpha-amylase inhibitor from bran [correction of brain] of rice (oryza sativa l.) seeds. | a 20 kda bifunctional inhibitor of the microbial proteinase, subtilisin, and the alpha-amylase from the larvae of the red flour beetle (tribolium castaneum) was purified from bran of rice seeds by saline extraction, precipitation with ammonium sulphate, ion-exchange chromatography on deae-cellulose and toyopearl cm-650, and preparative hplc on vydac c18. the complete primary structure was determined by automatic degradation of the intact, reduced and s-alkylated protein, and by manual dabitc/pit ... | 1992 | 1511747 |
| presence of three rice sucrose synthase genes as revealed by cloning and sequencing of cdna. | by sequencing cdna clones, we have concluded that three distinct sucrose genes are expressed in rice (oryza sativa cv. tainong 67). when the amino acid sequences deduced from these cdnas as well as those of known sucrose synthase are compared, the highest divergence is found in the c-termini. the most suitable dna sequences for use as specific for the mrna derived from these genes have been suggested. | 1992 | 1534703 |
| the hydroxyproline-rich glycoprotein gene from oryza sativa. | 1992 | 1536936 | |
| surface structure and properties of plant seed oil bodies. | storage triacylglycerols (tag) in plant seeds are present in small discrete intracellular organelles called oil bodies. an oil body has a matrix of tag, which is surrounded by phospholipids (pl) and alkaline proteins, termed oleosins. oil bodies isolated from mature maize (zea mays) embryos maintained their discreteness, but coalesced after treatment with trypsin but not with phospholipase a2 or c. phospholipase a2 or c exerted its activity on oil bodies only after the exposed portion of oleosin ... | 1992 | 1560029 |
| genomic structure of the gene for copper/zinc-superoxide dismutase in rice. | we have isolated and determined the nucleotide sequence of the structural gene (sodb) coding for one of the copper/zinc-superoxide dismutase (cu/zn-sod) isozymes from rice plants (oryza sativa l.). the sodb gene is split into 8 exons spread about 2 kb of the chromosomal dna, with the coding sequence beginning in the 2nd and ending in the 8th. although none of sodb introns are inserted into similar positions in cu/zn-sod genes from other sources, four of six introns in the protein-coding region a ... | 1992 | 1568478 |
| two rice (oryza sativa) full-length cdna clones encoding low-molecular-weight heat-shock proteins. | 1992 | 1581572 | |
| co-transcription of orf25 and coxiii in rice mitochondria. | southern hybridization analysis using homologous maize probes indicated that orf25 and coxiii are closely linked in the mitochondrial genome of rice (oryza sativa) cultivar ir36. the two coding regions were found on the same 5.1 kb bamhi fragment, and this fragment was cloned, mapped and partially sequenced. using probes for each gene derived from the rice clone, a 2.4 kb dicistronic mrna transcript was found containing both orf25 and coxiii coding regions. multiple 5' ends were identified by pr ... | 1992 | 1617739 |
| nucleotide sequences of two cdna clones encoding different cu/zn-superoxide dismutases expressed in developing rice seed (oryza sativa l.). | 1992 | 1623183 | |
| nucleotide sequence of the rice (oryza sativa) em protein gene (emp1). | 1992 | 1623185 | |
| characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene. | the promoter of the constitutively expressed rice (oryza sativa) actin 1 gene (act1) is highly active in transformed rice plants (w. zhang, d. mcelroy, and r. wu, plant cell 3:1150-1160, 1991). a region 834 bp upstream of the act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts (d. mcelroy, w. zhang, j. cao, and r. wu, plant cell 2:163-171, 1990). we have constructed a series of act1 promoter deletions fused ... | 1992 | 1630454 |
| isolation and molecular evolutionary analysis of a cytochrome c gene from oryza sativa (rice) | a cytochrome c gene, oscc-1, from rice (oryza sativa) has been isolated and analyzed. the oscc-1 gene encodes a cytochrome c protein that is typical of higher-plant cytochrome c proteins. oscc-1 consists of three exons separated by two introns that are 817 and 747 bp in length, respectively. from genomic dna hybridization analysis, oscc-1 appears to be one of possibly two cytochrome c genes in several asian, american, and indian rice species and varieties surveyed. a single, unique cytochrome c ... | 1991 | 1646373 |
| introduction and transposition of the maize transposable element ac in rice (oryza sativa l.). | to develop a transposon tagging system in an important cereal plant, rice (oryza sativa l.), the maize transposable element ac (activator) was introduced into rice protoplasts by electroporation. we employed a phenotypic assay for excision of ac from the selectable hph gene encoding resistance to hygromycin b. southern blot analysis of hygromycin b-resistant calli showed that the ac element can transpose from the introduced hph gene into the rice chromosomes. sequence analysis of several ac exci ... | 1991 | 1650907 |
| the isolation and characterisation of plant sequences homologous to human hypervariable minisatellites. | we have isolated dna probes, homologous to the human hypervariable minisatellite sequence 33.15, from the genome of rice (oryza sativa). these probes are capable of producing a multilocus rice dna fingerprint. the rice sequence has a tandem repeating structure based on a 12 bp gc-rich repeat which shows homology to its human counterpart. this probe detects up to 30 loci which are at a number of unlinked chromosomal sites. the gc-rich sequence is invariably associated with an open reading frame ( ... | 1991 | 1651259 |
| diversification of the rice waxy gene by insertion of mobile dna elements into introns. | the waxy (wx) gene of oryza glaberrima was cloned, and its nucleotide sequence was determined. a waxy mutant of o. glaberrima showing a glutinous phenotype was found to contain a substitution mutation generating a termination codon in the coding region of the wx gene. the wx sequence of o. glaberrima was different from that of oryza sativa by substitutions and insertions/deletions, among which only a few substitutions occurred in several exons not to severely alter the amino acid sequence of the ... | 1991 | 1685658 |
| transgenic rice cell lines and plants: expression of transferred chimeric genes. | cell suspension-derived rice (oryza sativa l.) protoplasts were transformed by direct gene uptake. peg-mediated transformation was more efficient than electroporation. plasmid dna containing a hygromycin phosphotransferase (hpt) gene (which confers hygromycin resistance) driven by the camv 35s promoter and a beta-d-glucuronidase (gus) gene under control of the 1', 2' double promoter of the mannopine synthase (mas) locus of agrobacterium tumefaciens was introduced into rice protoplasts. southern ... | 1991 | 1713479 |
| a novel ras-related rgp1 gene encoding a gtp-binding protein has reduced expression in 5-azacytidine-induced dwarf rice. | exposure of normal, tall rice (oryza sativa) seedlings to 5-azacytidine, a powerful inhibitor of dna methylation in vivo, induced both demethylation of genomic dna and dwarf plants. genes that had been affected by treatment were identified by differential screening of a cdna library, and a ras-related gene, rgp1, was subsequently isolated. the cdna of rgp1 was found to encode a deduced protein sequence of 226 amino acids with a relative molecular mass of 24850, which was most closely related to ... | 1991 | 1715972 |
| a novel glycine-rich cell wall protein gene in rice. | a genomic clone isolated from rice (oryza sativa var. ir 36) contains a gene that encodes a glycine-rich cell wall protein. this gene is a member of a multi-gene family. two transcripts with different 5' termini are encoded by this gene and the expression of these two transcripts is differentially regulated. the amino acid sequence derived from the dna sequence of this gene has a putative amino terminal signal peptide, and a highly repetitive structure in the putative mature protein. a model is ... | 1991 | 1716496 |
| nucleotide sequence of a high-pi rice (oryza sativa) -amylase gene. | 1992 | 1731997 | |
| identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. | chitinase isolated from zea mays seeds is inactivated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (edc) in the absence of exogenous nucleophiles. oligomers of n-acetylglucosamine,n,n',n",n"'-tetra-n-acetylchitotetraose (glcnac4), and to a lesser extent, n,n',n"-tri-n-acetylchitotriose (glcnac3) and n,n'-di-n-acetylchitobiose (glcnac2) provide partial protection against inactivation by the reagent. an examination of the concentration dependence of the protection afforded by glcnac4 revealed ... | 1992 | 1740436 |
| molecular identification and comparison of the starch synthase bound to starch granules between endosperm and leaf blades in rice plants. | normal (nonglutinous) rice plants (oryza sativa and o. glaberrima) contain more than 18% amylose in endosperm starch, while waxy (glutinous) plants lack it in this starch. in contrast, leaf starch contained more than 3.6% amylose even in waxy plants. sds-page analysis of proteins bound to endosperm starch granules in the normal plants revealed a single band with a mr of 60 kd, whereas waxy plants did not exhibit a similar band. the activity of starch synthase (ndp-glucose-starch glucosyltransfer ... | 1991 | 1747094 |
| dietary exposure to aflatoxin in benin city, nigeria: a possible public health concern. | a total of 100 samples of various foods comprising of 10 samples each of garri (manihot utilis pohl), beans (phaseolus lunatus), yam flour (dioscorea rotundata), cassava flour (manihot esculentum), melon (citrulus lunatus), onion (allium cepa), rice (oryza sativa), plantain (musa paradisiaca), red pepper (capsicum annuum l., solanaceae) and eggs were screened for the presence of aflatoxins. fifty percent of yam flour; 40% of cassava flour; 30% of garri; 20% of beans and melon and 10% of rice yie ... | 1991 | 1777386 |
| oryzacystatins as the first well-defined cystatins of plant origin and their target proteinases in rice seeds. | two cystatins occur in mature seeds of the rice, oryza sativa l. japonica, which are named oryzacystatin i (oc-i) and oryzacystatin ii (oc-ii). these are highly homologous to each other and are significantly homologous to cystatin superfamily members of animal origin, especially to family-2 cystatins. however, both lack disulfide bonds as in the case of family-1 cystatins (stefins). each of oc-i and oc-ii thus seems to be chimerical of family-1 and family-2 cystatins, and we propose that a new c ... | 1991 | 1801735 |
| organ-dependent regulation of a plant promoter isolated from rice by 'promoter-trapping' in tobacco. | a vector containing a transcriptionally inactive neomycin phosphotransferase ii gene was used to select promoter sequences from a pool of random genomic dna fragments. this paper describes how one such sequence (p4.7) isolated from oryza sativa acts as a hormonally regulated promoter in nicotiana tabacum. relative expression ratios in leaf, root, midrib, callus, and stem tissue of tobacco plants are 1:5:4:10:17. histochemical assays show that p4.7 activates the uida reporter gene throughout the ... | 1991 | 1844878 |
| differential expression of alpha-amylase genes in germinating rice and barley seeds. | steady-state levels of mrna from individual alpha-amylase genes were measured in the embryo and aleurone tissues of rice (oryza sativa) and two varieties of barley (hordeum vulgare l. cv. himalaya and cv. klages) during germination. each member of the alpha-amylase multigene families of rice and barley was differentially expressed in each tissue. in rice, alpha-amylase genes displayed tissue-specific expression in which genes ramy3b, ramy3c, and ramy3e were preferentially expressed in the aleuro ... | 1991 | 1859866 |
| localization of specific repetitive dna sequences in individual rice chromosomes. | this paper describes the characterization and chromosomal distribution of three different rice (oryza sativa) repetitive dna sequences. the three sequences were characterized by sequence analysis, which gave 355, 498 and 756 bp for the length of the repeat unit in os48, osg3-498 and osg5-756, respectively. copy number determination by quantitative dna slot-blot hybridization analysis showed 4000, 1080 and 920 copies, respectively, per haploid rice genome for the three sequences. in situ dna hybr ... | 1991 | 1860377 |
| a homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex i is encoded in chloroplast genomes. | the chloroplast genomes of marchantia polymorpha, nicotiana tabacum, and oryza sativa contain open reading frames (orfs or potential genes) encoding homologues of some of the subunits of mitochondrial nadh:ubiquinone oxidoreductase (complex i). seven of these subunits (nd1-nd4, nd4l, nd5, and nd6) are products of the mitochondrial genome, and two others (the 49- and 30-kda components of the iron-sulfur protein fraction) are nuclear gene products. these findings have been taken to indicate the pr ... | 1991 | 1901022 |
| organization of dna sequences highly repeated in tandem in rice genomes. | digestion of the total genomic dna from rice oryza sativa l. cv. c5924 with ecori generated an intense band of a dna fragment of about 0.36 kb long. the dna fragment cloned into puc19 was used to hybridize with the total rice genomic dna partially digested with ecori. a ladder of bands of dna fragments with multiplied length of 0.36 kb was observed, demonstrating that this sequence occurs in tandem in the genome. the copy number of the sequence estimated by dot blot hybridization analysis was 20 ... | 1991 | 1910873 |
| cloning and characterization of repetitive dna sequences from genomes of oryza minuta and oryza australiensis. | the value of genome-specific repetitive dna sequences for use as molecular markers in studying genome differentiation was investigated. five repetitive dna sequences from wild species of rice were cloned. four of the clones, pom1, pom4, poma536, and pompb10, were isolated from oryza minuta accession 101141 (bbcc genomes), and one clone, poa237, was isolated from oryza australiensis accession 100882 (ee genome). southern blot hybridization to different rice genomes showed strong hybridization of ... | 1991 | 1955157 |
| magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. | we have identified genes for pathogenicity toward rice (oryza sativa) and genes for virulence toward specific rice cultivars in the plant pathogenic fungus magnaporthe grisea. a genetic cross was conducted between the weeping lovegrass (eragrostis curvula) pathogen 4091-5-8, a highly fertile, hermaphroditic laboratory strain, and the rice pathogen o-135, a poorly fertile, female-sterile field isolate that infects weeping lovegrass as well as rice. a six-generation backcrossing scheme was then un ... | 1991 | 2016048 |
| isolation and characterization of a rice gene encoding a basic chitinase. | chitinase, which catalyzes the hydrolysis of the beta-1,4-n-acetyl-d-glucosamine linkages of the fungal cell wall polymer chitin, is involved in inducible defenses of plants. a basic chitinase genomic sequence was isolated from a rice (oryza sativa l.) genomic library using a bean chitinase gene fragment as a probe. the complete nucleotide sequence of the rice chitinase rch10 gene was determined, and shown to contain an open reading frame with no introns, encoding a polypeptide of 336 amino acid ... | 1991 | 2034221 |
| nucleotide sequence of citrus limon 26s rrna gene and secondary structure model of its rna. | the complete nucleotide sequence of citrus limon 26s rdna has been determined. the sequence has been aligned with large ribosomal rna (l-rrna) sequences of escherichia coli, saccharomyces cerevisiae and oryza sativa. nine extensive expansion segments in dicot 26s rrna relative to e. coli 23s rrna have been identified and compared with analogous segments of monocot, yeast, amphibian and human l-rrnas. a secondary structure model for lemon 26s rrna has been derived based on the refined model of e. ... | 1990 | 2101688 |
| genomic nucleotide sequence of four rice (oryza sativa) actin genes. | 1990 | 2102841 | |
| characterization of the rice (oryza sativa) actin gene family. | four rice (oryza sativa) genomic actin genes have been characterized. the rice actin genes show a conservation of intron number and position that is characteristic of plant actins. sequence comparisons revealed that the plant actins generally have a high degree of inter- and intraspecific sequence heterogeneity. however, one rice actin gene has a much higher degree of nucleotide sequence identity to a previously isolated actin sequence from arabidopsis thaliana than to any other plant actin gene ... | 1990 | 2103449 |
| plant regeneration from protoplasts of a super chinese rice (oryza sativa l.) cultivar--zhonghua no.8. | green rice plants were regenerated from protoplasts, which were derived from cell suspension of oryza sativa l. cut. zhonghua no. 8, a super chinese rice cultivar with high productivity, good quality and high resistance to both bacterial blight and blast. the embryogenic calli were initiated from mature embroys. it took about 4 months to establish the cell suspenion. the regeneration plants from protoplasts were obtained in 2 months after the isolation of protoplasts. | 1990 | 2104199 |
| nucleotide sequences of mitochondrial atpase subunit 9 genes from three lines of rice (oryza sativa l.) | 1990 | 2143016 | |
| recognition efficiency of dicotyledoneae-specific promoter and rna processing signals in rice. | heterologous gene expression experiments have shown that genes of monocotyledoneae are often not transcribed in dicotyledoneae, or produce pre-mrna that is inefficiently or aberrantly processed. it is however not known how correctly and efficiently dicotyledon-specific gene expression signals are recognized in cells of monocotyledoneae. here we address this question using tobacco (nicotiana tabacum) and rice (oryza sativa) protoplasts transformed with the same hybrid gene constructs. constructs ... | 1990 | 2177137 |
| a repeated chromosomal dna sequence is amplified as a circular extrachromosomal molecule in rice (oryza sativa l.). | the plasmid pe10 is a pbr322-derived plasmid carrying a 4.5 kb rice (oryza sativa l.) repeated dna sequence. the cloned sequence has been shown to be amplified in cultured rice cells. the analysis of practically intact chromosomal rice dna molecules by pulsed field gel electrophoresis has now shown that the amplification is associated with the appearance of extrachromosomal molecules. in fact, pe10 hybridizes exclusively with unfractionated dna from leaf protoplasts, while it recognizes predomin ... | 1990 | 2233681 |
| proteins and amino acids of some local varieties of rice seeds (oryza sativa l.). | proteins and amino acids in four local rice (oryza sativa l.) varieties were identified. albumin and globulin were extracted from rice seeds, and the major promoters of these proteins were investigated by polyacrylamide gel electrophoresis to show their patterns. amino acid composition of the rice seed were determined quantitatively and qualitatively, and classified according to their acidic, basic and uncharged polar groups. essential amino acids for each variety were determined, and the hydrop ... | 1990 | 2247436 |
| fingerprinting genomes using pcr with arbitrary primers. | simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (pcr). no prior sequence information is required. the method, arbitrarily primed pcr (ap-pcr), involves two cycles of low stringency amplification followed by pcr at higher stringency. we show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. the generality of the method is demonstrated by application to twenty four ... | 1990 | 2259619 |
| purification and partial characterization of pyruvate decarboxylase from oryza sativa l. | pyruvate decarboxylase(pyrdc) was purified from rice bran to a specific activity of 1 mu kat/mg and partially characterized. the holoenzyme is a tetramer of two types of subunits with molecular masses 64 kda and 62 kda. purified rice pyrdc exhibits positive cooperative kinetics with respect to pyruvate and functions with a significant lag phase. when compared to other plant pyrdc, the lag phase was shorter at low pyruvate concentrations and the s0.5 was smaller. the optimum ph (6.25) was also le ... | 1990 | 2269301 |
| computer analysis of phytochrome sequences from five species: implications for the mechanism of action. | the amino acid sequences of phytochrome from avena sativa, oryza sativa, curcurbita pepo, pisum sativum and arabidopsis thaliana have been analyzed with a variety of computer programs, with a view to identifying areas of the protein which contribute to the properties of this photoreceptor. a region at the c-terminus has been shown to be amphiphilic, and by analogy with surface-seeking peptides, may be responsible for interaction of phytochrome with lipid bilayers. possible targeting sequences in ... | 2009 | 2291773 |
| the genic nature of gamete eliminator in rice. | the two cultivated rice species, oryza sativa and oryza glaberrima, are morphologically alike but are reproductively isolated from each other by hybrid sterility. the hybrid is male sterile but partially female fertile. backcross experiments were conducted to introduce an alien factor controlling hybrid sterility from o. glaberrima (w025) into o. sativa (t65wx) and examine the genetic basis. an extracted sterility factor, closely linked to the wx locus, induced gametic abortion due to allelic in ... | 1990 | 2341030 |
| food-based solutions are a viable alternative to glucose-electrolyte solutions for oral hydration in acute diarrhoea--studies in a rat model of secretory diarrhoea. | a survey of acute diarrhoea and its treatment, in 3 groups of villages in south india, revealed that use of the world health organization oral rehydration solution (who-ors) was poor or virtually non-existent and that several liquid foods were given to children during acute diarrhoea. the effects of the most commonly used, boiled and cooled supernatants of these liquid foods [rice (oryza sativa)-water, ragi (eleusine coracana)-water, arrowroot (maranta arundinacea)-water], and tender coconut-wat ... | 1990 | 2345922 |
| the alpha-amylase genes in oryza sativa: characterization of cdna clones and mrna expression during seed germination. | two cdna clones, pos103 and pos137, were isolated which code for distinct alpha-amylase isozymes in germinating rice seeds. sequence analysis indicated that the clones encode polypeptides of approximately 48 kda, both of which possess a signal peptide involved in directing secretion of the protein. comparison of the two rice alpha-amylase amino acid sequence showed that they are 76% similar to each other, while showing 85% to 90% similarity with other cereal alpha-amylases. a comparison of eleve ... | 1990 | 2370848 |
| mrnas and a cloned histone gene are differentially expressed during anther and pollen development in rice (oryza sativa l.). | spatial and temporal changes in the distribution of mrna sequences during anther and pollen development in rice (oryza sativa) were investigated by in situ hybridization with [3h]polyuridylic acid ([3h]poly(u)) and a cloned rice histone gene probe. annealing of sections with [3h]poly(u) showed that poly(a)-containing rna (poly(a)+rna) was uniformly distributed in the cells of the anther primordium. during the formation of the archesporial initial, the primary parietal cell, the primary sporogeno ... | 1989 | 2476454 |
| the nucleotide sequences and expression of genes for the beta and epsilon subunits of atp synthase from rice (oryza sativa l.) | the genes for the beta and epsilon subunits of atp synthase were cloned from rice chloroplast dna. the nucleotide sequences and the deduced amino acid sequences of these genes were determined. the site of initiation of transcription was examined by s1-mapping analysis. the relation between stability of mrna and that of a stem and loop structure at the 3' end of mrna is discussed. | 1989 | 2534354 |
| [characterization of a cloned dna fragment of rice chloroplast and location rbcl gene in the recombinant plasmid]. | the pure rice (oryza sativa, subsp. indica, cv. zhenshan 97b) chloroplast dna was digested by restriction enzyme bamhi and the resulting fragments were ligated to the bamhi site of pbr322. one recombinant plasmid which contains a 19.3kb insertel dna fragment was isolated from the clone bank and was named posb1. a physical map of the recombinant plasmid was constructed by cleavage with ten restriction endonucleases, and the gene rbcl was located on the posb1. | 1989 | 2561754 |
| three cdna sequences coding for glutamine synthetase polypeptides in oryza sativa l. | 1989 | 2577497 | |
| heavy metal tolerance of rice plants (oryza sativa l.) to some metal oxides at the critical levels. | the toxicity of metal oxides (cdo, zno, pbo) were compared with each other and the critical levels of metal uptake by rice plants were determined. the order of metal toxicity to rice plants is cdo greater than zno greater than pbo. the highest concentration obtained for unpolished rice was 4.97 micrograms/g at the cd 10,000 ppm in soil. this values is higher than every other we have seen in the reports for treatment with cdo. we are able to find out that the concentration of 10,000 ppm cd in the ... | 1989 | 2600364 |
| [larval density of anopheles (n) albimanus wiedemann, 1821 in relation to phenology of oryza sativa (linnaeus) rice]. | the larval density of anopheles (n) albimanus wiedemann, 1821, is linked with rice phenology in the two crops of the year (summer and winter). it was determined that a higher density agrees with the second stage of the vegetative cycle of the culture named beginning of shooting processes and the formation of the panicular primordium. the number of anopheline larvae was higher in the summer. | 1989 | 2672175 |
| partial proteolysis of rice phytochrome: comparison with oat phytochrome. | phytochrome was isolated from etiolated rice seedlings (oryza sativa l.) by slight modification of the procedure for oat phytochrome. spectral data of rice phytochrome are comparable with those of oat and rye phytochrome. controlled proteolysis with endoproteinases lys-c and glu-c yielded defined fragments some of which were different for pr and pfr. the fragments were identified by comparison with the corresponding fragments of oat phytochrome and by comparison of the amino acid sequences of ri ... | 2015 | 2686670 |
| structure and expression of the rice glutelin multigene family. | a near full-length cdna and three genomic clones for rice (oryza sativa l.) glutelin were isolated and studied. based on nucleic acid sequence and southern blot analyses, the three isolated glutelin genomic clones were representative members of three gene subfamilies each containing five to eight copies. a comparison of dna sequences displayed by relevant regions of these genomic clones showed that two subfamilies, represented by clones gt1 and gt2, were closely related and evolved by more recen ... | 1989 | 2745459 |
| testing of genotoxic effects of 2,4-dichlorophenoxyacetic acid (2,4-d) using multiple genetic assay systems of plants. | the mutagenic activity of 2,4-d was assessed utilising cytogenetic, chlorophyll mutation, specific-locus and pollen viability endpoints in allium cepa and oryza sativa. in the allium root-tip assay onion bulbs with roots were treated with 25, 50, 75 or 100 ppm 2,4-d for 4 h and the frequency of aberrations was estimated. with an increase in concentration there was rise in frequency of aberrant cells. in the case of mutation assays, presoaked rice seeds were treated with 100, 200 or 300 ppm 2,4-d ... | 1989 | 2761564 |
| the complete sequence of the rice (oryza sativa) chloroplast genome: intermolecular recombination between distinct trna genes accounts for a major plastid dna inversion during the evolution of the cereals. | the entire chloroplast genome of the monocot rice (oryza sativa) has been sequenced and comprises 134525 bp. predicted genes have been identified along with open reading frames (orfs) conserved between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (nicotiana tabacum), and a liverwort (marchantia polymorpha). the same complement of 30 trna and 4 rrna genes has been conserved between rice and tobacco. most orfs extensively conserved between n. tabacum and m. polymorpha ar ... | 1989 | 2770692 |
| the amino acid sequence of plastocyanin from rice (oryza sativa, subspecies japonica). | the amino acid sequence of plastocyanin from rice (subspecies japonica) was determined. it consists of a single polypeptide chain of 97 residues and has a molecular mass of 10,225 da. the sequence shows 94.8% identity with barley plastocyanin. | 2009 | 2780537 |
| nucleotide sequence of the rice cytochrome f gene and the presence of sequence variation near this gene. | the rice (oryza sativa l. var. labelle) chloroplast (cp) gene encoding cytochrome f has been isolated and sequenced. the coding region of this rice gene displays 95.1%, 85.3% and 85.2% nucleotide sequence homology with that of wheat, pea and spinach, respectively. to examine the cpdna sequence variation in rice, cpdna from labelle and its parents, belle patna and dawn was compared. using the cytochrome f gene as the probe for hybridization, we found several differences in the size and number of ... | 1986 | 2884170 |
| long-term effects of land application of aqueous oil effluent on photosynthetic efficiency of certain varieties of oryza sativa l. | 1989 | 2920225 | |
| inactivation of alcohol dehydrogenase in rice seedlings is related to a microsomal fatty acid alpha-oxidation system. | the selective inactivation of alcohol dehydrogenase by the inactivator found in the microsomal fraction of rice (oryza sativa) seedlings growing in air (shimomura, s. & beevers, h. (1983) plant physiol. 71, 736-741; 742-746) was further studied. this inactivation was found to be essentially dependent on the presence of free fatty acids. the specificity for fatty acids and the inhibitory effects of imidazole, 2-hydroxyfatty acids and dithiothreitol on the inactivation were all consistent with the ... | 1986 | 2939066 |
| sequence of the chloroplast psbf gene encoding the photosystem ii 10 kda phosphoprotein from oryza sativa l. | 1988 | 3057446 | |
| temperature and the cell cycle. | during the period between successive divisions, a cell traverses three stages of interphase: g1 (pre-synthetic interphase), s-phase (dna synthetic interphase) and g2 (post-synthetic interphase). the time taken for all cells in a meristem to divide (the cell doubling time (cdt] decreases in response to an increase in temperature. for example, the cdt in root meristems of zea mays decreases 21-fold as the temperature is increased from 3 to 25 degrees c. whether all phases of the cell cycle alter p ... | 1988 | 3077857 |
| effect of sugar mill effluent on oxygen uptake and carbon dioxide output of rice (oryza sativa l.c.v. mushoori) seedlings. | the effects of a sugar mill effluent on respiration of rice (oryza sativa l.c.v. mushoori) seedlings have been investigated. each experiment consisted of two parts, the effect of various concentrations of the effluent and the time-dependent changes induced by the undiluted effluent. a marked increase in the rate of respiration was noticed upon treatment with various concentrations of the effluent. the time-dependent changes in the respiratory rate were about threefold with the 12 hr of treatment ... | 1987 | 3107978 |
| the effect of sugar mill effluent on enzyme activities of rice seedlings. | the effect of sugar mill effluent on the activities of peroxidase, amylase, and nitrate reductase of rice (oryza sativa l. c.v. mushoori) seedlings have been investigated. in addition, an attempt was also made to investigate effluent-induced changes in the activities of mitochondrial enzymes, such as succinate dehydrogenase, during germination of rice seedlings. the effects were studied in relation to both concentration of effluent and time of exposure. the activity of all enzymes tested, except ... | 2011 | 3160578 |
| interaction of a gibberellin-induced factor with the upstream region of an alpha-amylase gene in rice aleurone tissue. | the interaction between the dna sequences of an alpha-amylase (ec 3.2.1.1) gene and a tissue-specific factor induced in rice (oryza sativa l.) aleurone tissue by gibberellin was studied. dna mobility-shift during electrophoresis indicated that a 500-base-pair sequence (hs500) of a rice alpha-amylase genomic clone (osamy-a) specifically interacted with a factor from gibberellin-induced rice aleurone tissue. the amount of complex formed between the hs500 dna fragment and the gibberellin-induced fa ... | 1988 | 3261867 |
| interaction of asparagine-linked oligosaccharides with an immobilized rice (oryza sativa) lectin column. | the carbohydrate-binding specificity of rice (oryza sativa) lectin was investigated by testing the ability of radioactively labelled glycopeptides and oligosaccharides to bind to a rice lectin-sepharose 4b column. rice lectin binds asparagine-linked oligosaccharides through the core nn'-diacetylchitobiose moiety. whereas beta 1-4-mannose enhances the binding strength only to a small extent, alpha 1-3-linked core mannose increases it considerably. a core alpha 1-6-linked mannose residue has a sli ... | 1988 | 3355507 |
| isolation and hypoglycemic activity of oryzabrans a, b, c and d, glycans of oryza sativa bran. | 1988 | 3375329 | |
| chromosomal locations of ten isozyme loci in rice (oryza sativa l.) through trisomic analysis. | chromosomal locations of 10 isozyme loci in rice (oryza sativa l.) were determined through trisomic analysis. all 10 genes produced altered allozyme banding patterns in specific f1 trisomics. this served as the primary source of evidence for chromosome locations of est-5, icd-1, acp-1, and pgd-1. the locations of amp-1, amp-2, amp-4, pox-5, got-1, and cat-1 were further confirmed from segregation data in bc1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but ... | 1988 | 3408480 |
| isolation and hypoglycemic activity of oryzarans a, b, c, and d: glycans of oryza sativa roots. | 1986 | 3562666 | |
| the effects of fruit juices and fruits on the absorption of iron from a rice meal. | the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ... | 1987 | 3593665 |
| in vitro segregation of marker genes in anther cultures of rice. | segregation of genes controlling expression of anthocyanin pigmentation in rice (oryza sativa l. subsp. indica) leaf blade and leaf sheath was examined in the microspore-derived plants. the segregation pattern of marker genes was found to fit closely the expected gametic segregation ratios among microspore-derived green as well as albino plants. microspore-derived in vitro regenerated plants expressed genetic traits similar to seedlings. the results indicate that the germ cell culture technique ... | 1987 | 3610948 |
| biochemical and immunological characterization of rice albumin. | rice albumin from oryza sativa (var. basmati 370) accounts for about 5% of the total seed proteins. a major fraction of rice albumin has been found to be a glycoprotein which is a monomer of 60 kd having iso-electric point 6.54. when rice albumin is digested with trypsin, it shows the presence of 24 peptides as against 28 peptides which were estimated from its amino acid composition. this indicates the presence of a few peptides which resemble each other in their charge and rf values. antibodies ... | 1987 | 3620599 |