Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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[isolation and characterization of main albumin fractions of seeds from sunflower (helianthus annuus l.) and rape (brassica napus l.)]. | the main fractions of the albumins from sunflower and rapeseeds (isolated by means of precipitation with ammonium sulphate or tannin + caffeine and subsequent gel chromatography) are low-molecular, very basic proteins. their molecular weights range from 10 000 to 16 000, and their isoelectric points (determined by isoelectric focusing and free electrophoresis) are situated at ph greater than 10.0. from the circular dichroism in the wavelength range from 200 to 240 nm it is deduced that the main ... | 1975 | 5674 |
[quinic and isochlorogenic acids in sunflower seeds (helianthus annuus lin.)]. | quinic and isochlorogenic acids have been identified in sunflower's seeds (helianthus annuus lin.) by paper chromatography in presence of various solvents. quinic acid has been detected by color reaction and isochlorogenic by ultra-violet light. | 1975 | 131623 |
enzymic capacities for chlorophyll biosynthesis. activation and de novo synthesis of enzymes. | a previously published working model for the regulation of chlorophyll formation has been tested studying early steps of chlorophyll and porphyrin biosynthesis in developing cotyledons of helianthus annuus. the activities of delta-aminolevulinate synthetase (alas), delta-aminolevulinate dehydratase (alad), and the porphobilinogenase complex (pbgase) at any given time have been found to be strongly associated with endogenous developmental processes. highest activities in darkness have been observ ... | 1976 | 132041 |
purification and characterization of the crown gall specific enzyme nopaline synthase. | nopaline synthase of sunflower (helianthus annuus l.) crown gall tissue induced by agrobacterium tumefaciens strain c58 or t37 (nopaline utilizers) was purified to homogeneity as judged by analytical disc gel electrophoresis. the native enzyme elutes from a column of ultrogen aca 34 as a single peak with an estimated molecular weight of 158,000. the dissociated enzyme migrates on nadodso4-polyacrylamide gels as a single band with a molecular weight of 40,000. thus, the native enzyme appears to b ... | 1979 | 476084 |
dry mass content in nuclei and cytoplasm during differentiation of the root cortex cells of helianthus annuus. | dry mass content in nuclei and cytoplasm as well as its concentration during the growth and differentiation of the root cortex cells of helianthus annuus were investigated by means of interference microscopy. moreover, by incubation with radioactive thymidine, the endopolyploidization at the level of 4c was found to exist in this species. it was found that during the growth and differentiation of the root cortex cells, the average dry mass content in nuclei essentially does not increase, whereas ... | 1979 | 488863 |
dissociation-recombination of sunflower seed acid phosphatase. | formal genetic studies of sunflower (helianthus annuus) seed acid phosphatase (acp, e.c. 3.1.3.2) had suggested that the functional enzyme consists of two polypeptide subunits. the dimeric quaternary structure was demonstrated by dissociation-recombination procedures. dissociation of electrophoretically distinct homodimers was effected upon freezing of extracts in a ph 8-9 buffer containing 1 m nacl and 0.1 m 2-mercaptoethanol. reassociation, as indicated by the formation of the hybrid isozyme, ... | 1977 | 588237 |
[analysis of the quaternary structure of the 11s plant globulins by means of small-angle x-ray scattering]. | it is demonstrated by means of small-angle x-ray scattering that 11 s globulin of sunflower (helianthus annuus l.) consists of six subunits. the point group symmetry of the quaternary structure is 23 (d3). it is postulated that this symmetry is typical for the group of 11 s seed proteins. | 1978 | 706920 |
[nutritive value of sunflower seeds (helianthus annus) (author's transl)]. | the nutritive value of sunflower seeds (helianthus annus) was studied and compared to that of casein. thirty-six male rats, wistar strain, aged 23 days, were divided into two groups and fed (a) a sunflower cake meal diet, and (b) a casein diet. the results indicate that due to its high protein level (52.06%) and coefficient of digestibility (95 cd) sunflower can be used as a feeding formula although its per does not recommend its use as a milk substitute. | 1977 | 905604 |
[stability of oil from the sunflower helianthus annuus l. action of antioxidants]. | 2006 | 1030513 | |
[reaction of the seedlings of flowering plants to the effects of imino-di-(paradimethylaminophenyl)-methane-aurine-00]. | the influence of auramine-00 on seedlings of three species of plants - cucurbita pepo l., helianthus annuus l., zea mays l.-was investigated. auramine was added in agar-agar, on which the seedlings were growing. two concentrations of auramine were used--0.001%. besides the delay in growth, also the formation of local overgrowth of cotyledons and hypocotyl of c. pepo, the base of cotyledons of h. annuus and mezocotyl of z. mays were observed. the histological analysis has shown these swellings to ... | 1975 | 1166654 |
[response of flowering plant seedlings to the action of n-nitroso-n-methylurea]. | the influence of n-nitroso-n-methylurea on seedlings of two species of plants--cucurbita pepo l., helianthus annuus l.--was investigated. nmu was added in agar-agar, on which seedlings were growing. 0.02% concentration of nmu was used. in addition to the delay in growth and appearance of necrotic regions, also local overgrowth of cotyledons of c. pepo and overgrowth of hypocotyl and the base of cotyledons of h. annuus were observed. a histological analysis has shown that these swellings arise du ... | 1975 | 1210149 |
assay of nitrogenase activity in intact plant systems. | nitrogenase activity was assayed in intact system of cichorium intybus, a non-leguminous commercially cultivated crop, dahlia pinnata and helianthus annus, and taraxacum officinale, a common weed plant. the assay was made in fabricated cylinders which could accomodate pot with plants. in such kind of assay along with rhizosphere microflora, the nitrogen fixed by phyllosphere nitrogen fixing microflora could also be accounted, which otherwise was difficult to be accounted for. | 1975 | 1211718 |
[isolation and characterization of 11-s-globulins from sunflower seeds (helianthus annuus l.)]. | the isolation of ii-s globulin, which is homogenous on sedimentation and gel chromatography (s020, w = ii,8 s), from sunflower seeds is achieved by cryoprecipitation from solutions of high ion strength (i approximately 0.5 to 1.8) with simultaneous reduction of the salt concentration (i approximately 0.3) and repeated gel chromatography on sephadex g-200. by this technique, the 7-s component which occurs in small quantity is isolated too. in solutions of low ion strength (i less than 0,3), the i ... | 1975 | 1233024 |
dissociation-recombination of intergenic sunflower alcohol dehydrogenase isozymes and relative isozyme activities. | two unlinked genes, adh1 and adh2, control of the production of alcohol dehydrogenase (adh) in seeds of the animal sunflower (helianthus annuus). each gene is polymorphic, having f and s alleles. starch gel electrophoretic zymograms of the four possible double homozygotes have three bands, representing two homodimers and an intermediately migrating intergenic isozyme. zymograms of double heterozygotes consist of nine bands produced by ten isozymes: six intragenics and four intergenics, two of wh ... | 1976 | 1259707 |
a specifc inactivator of glyoxysomal isocitrate lyase from sunflower (helianthus annuus l.) cotyledons. | 1976 | 1278372 | |
characterization of proton extrusion in sunflower cell cultures. | cell suspensions derived from callus root tips of sunflower (helianthus annuus l., cv. enano) were obtained in order to assess the effects of different chemical and physical agents on cell h+ extrusion. cell h+ efflux was sensitive to temperature, ph, inhibitors of plasmalemma h(+)-atpase and ca2+ and k+ concentrations in the assay medium, as well as to the light intensity at which cells were cultivated. thus, in the darkness and at 60 mumol/m2/s of illumination, a strong inhibition of h+ extrus ... | 1992 | 1329160 |
molecular analysis of a new cytoplasmic male sterile genotype in sunflower. | mitochondrial dna from 1 fertile and 6 cytoplasmic male sterile (cms) sunflower genotypes was studied. the cms genotypes had been obtained either by specific crosses between different helianthus species or by mutagenesis. cms-associated restriction fragment length polymorphisms (rflps) were found in the vicinity of the atpa locus, generated by various restriction enzymes. the organization of the mitochondrial genes 26s rrna, 18s + 5s rrna and coxii was investigated by southern blot analysis. the ... | 1992 | 1348035 |
toxicity of some heavy metals in vivo and in vitro in helianthus annuus. | to compare the toxicity of some heavy metals in vivo and in vitro, the effects of six metals, aluminum (al), cadmium (cd), copper (cu), nickel (ni), lead (pb), and zinc (zn) were studied in the oil-yielding plant helianthus annuus. the percentage of seed germination and cytotoxic effects at different concentrations and durations of treatment as well as the growth rate of callus tissues in vitro were compared to ascertain the concentrations that can either support plant growth or cause lethality. ... | 1992 | 1383802 |
cdna sequence of a sunflower oleosin and transcript tissue specificity. | oleosins (oil body membrane proteins) of 20.5 and 18 kda have been purified from sunflower (helianthus annuus) seeds and polyclonal antibodies raised against them. the precipitated rabbit immunoglobulin fraction was purified by affinity chromatography on cyanogen bromide-activated sepharose and specifically recognised polypeptides of 18 and 20.5 kda in sunflower homogenate and oil body fractions assayed by western blotting. a near-full-length cdna clone was isolated for the 20.5 kda oleosin. the ... | 1992 | 1643288 |
mitochondrial genome organization and expression associated with cytoplasmic male sterility in sunflower (helianthus annuus). | a comparative investigation of the organization and expression of the mitochondrial genome in fertile and cytoplasmic male sterile (cms) sunflower (helianthus annuus) has been undertaken. a region of mitochondrial genome variation between the two phenotypes has been located in the 3' flanking region of the gene encoding the alpha subunit of the f1 atpase (atpa). physical mapping and sequence analysis have been used to show that a rearrangement involving an inversion and an insertion has occurred ... | 1991 | 1668966 |
cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpa gene. | the organization and expression of the mitochondrial (mt) genome of fertile, male-sterile and restored lines of helianthus annuus and of h. petiolaris were compared to identify alterations which might lead to cytoplasmic male sterility (cms). the mtdnas of fertile and male-sterile lines differ by an 11 kb inversion and a 5 kb insertion. the rearrangements seem to be the result of recombination events within an inverted repeat of 261 bp. detectable alterations in the transcript pattern of the rea ... | 1991 | 1714033 |
a mitochondrial 16 kda protein is associated with cytoplasmic male sterility in sunflower. | cytoplasmic male-sterile lines cms89 and cmsbaso of sunflower (helianthus annuus) differ from the fertile lines ha89 and baso in a mitochondrial dna sequence in the vicinity of the atpa gene. in addition, the transcriptional pattern of the atpa gene is changed in male-sterile lines compared to fertile ones. besides one main transcript in the fertile lines, the male-sterile lines additionally show larger transcripts. investigation of baso and cmsbaso revealed that the two fertility-restored lines ... | 1991 | 1714323 |
kinetic properties of phenylalanine ammonia-lyase from sunflower (helianthus annuus l.) hypocotyls. | phenylalanine ammonia-lyase (pal) from sunflower hypocotyls has been partially purified by selective precipitation with ammonium sulfate and molecular gel filtration on sephacryl s-300. kinetic assays carried out with this partially purified pal preparation revealed that the enzyme did not show a homogeneous kinetic behaviour. the observed kinetic pattern and parameters (km and vmax) depended on the assay conditions used and the protein concentration added to the assay mixture. pal displayed mic ... | 1991 | 1768248 |
[new triterpenoids saponins from helianthus annuus]. | three new bisdesmosidic triterpenoid saponins, helianthoside 1(1), 2(2), and 3(3), were isolated from the flowers of helianthus annuus l. and a new monodesmoside 4 was isolated after the cleavage of the ester-glycosidic linkages of 2 and 3. the structures of the compounds were elucidated by 13c-nmr, fab-ms, gc/ei-ms of partially methylated alditol acetates and degradation methods. | 1991 | 1798804 |
feline meningitis due to cryptococcus neoformans var. neoformans and review of feline cryptococcosis. | cryptococcus neoformans var. neoformans was ascertained as the prime cause of fatal meningitis in a young-adult female domestic cat who was suspected of rabies. the pathogen was isolated in heavy growth from the infected brain specimen on simplified sunflower seed (helianthus annuus) medium at 30 degrees c. periodic acid-schiff stained impression smear revealed numerous cryptococcal cells. histologically, pia-arachnoid showed thickening along with many circular yeast cells with and without buddi ... | 1991 | 1803233 |
amino acid and cdna sequences of a methionine-rich 2s protein from sunflower seed (helianthus annuus l.). | the amino acid sequence of a methionine-rich 2s seed protein from sunflower (helianthus annuus l.) and the sequence of a cdna clone which codes for the entire primary translation product have been determined. the mature protein consists of a single polypeptide chain of 103 amino acids (molecular mass 12133 da) which contains 16 residues of methionine and 8 residues of cysteine. the cdna sequence established that the protein is synthesized as a precursor of 141 residues with a typical hydrophobic ... | 1991 | 1997318 |
isolation of cryptococcus neoformans var. neoformans from canine otitis. | cryptococcus neoformans was demonstrated in the clinical material of a 15-year-old male dog with a history of chronic otitis externa by employing helianthus annuus seed agar as a selective medium. the examination of the isolate for sexual compatibility on modified helianthus annuus seed medium revealed that it belonged to filobasidiella neoformans var. neoformans 'alpha' mating type. in the authors' view, this appears to be the first report of isolation of cr. neoformans var. neoformans from dis ... | 1990 | 2093842 |
nucleotide sequence of two anther-specific cdnas from sunflower (helianthus annuus l.). | 1990 | 2102380 | |
rapid light-induced changes in phosphoinositide kinases and h(+)-atpase in plasma membrane of sunflower hypocotyls. | irradiation of sunflower (helianthus annuus l. cv. russian mammoth) hypocotyls with white light resulted in a 51% decrease in plasma membrane phosphatidylinositol monophosphate (pip) kinase activity. as little as 10 s of white light irradiation was sufficient to lower the phosphatidylinositol bisphosphate (pip2) produced in the in vitro phosphorylation assay. this decrease was not caused by an increase in phospholipase c activity since analysis of the water-soluble products indicated no increase ... | 2007 | 2168392 |
developmentally regulated expression of a sunflower 11s seed protein gene in transgenic tobacco. | helianthinin is the major 11s seed storage protein of sunflower (helianthus annuus). like most seed proteins, helianthinin is encoded by a small gene family; two members of this gene family, hag3-a and hag3-d, have been isolated and characterized. tobacco was transformed with a 6 kb fragment of hag3-a containing the helianthinin coding region flanked by 3.8 kb upstream and 0.4 kb downstream sequence. expression of helianthinin was developmentally regulated in seeds of transgenic tobacco plants; ... | 1990 | 2233680 |
plasma membrane purification from roots of sunflower by phase partitioning. | plasma membrane vesicles were purified from the roots of sunflower (helianthus annuus l. cv. topflor) by aqueous polymer two-phase partitioning. the optimal conditions for separation were determined by systematic variation of the polymer concentration and salt composition. the phase system containing 6% (w/w) dextran t-500, 6% (w/w) polyethylene glycol 3350, 250 mm sucrose, 5 mm potassium phosphate, ph 7.8, without added salts proved to be the best. the atpase activity had a ph optimum at 6.5 an ... | 1989 | 2535027 |
the primary structure of sunflower (helianthus annuus) ubiquitin. | 1989 | 2538802 | |
sunflower allergy. on the constituents of the trichomes of helianthus annuus l. (compositae). | sesquiterpene lactones (stl) of the sunflower, occurring in fragile multicellular capitate glandular hairs, have been shown to display a remarkable sensitizing capacity when investigated by experimental sensitization in guinea pigs. the strongest response was induced by the hemiketal form of 1-o-methyl-4,5-dihydroniveusin a. the stl content of 50 capitate glands was sufficient to elicit a remarkable response in the guinea pigs. thus, touching a sunflower plant (with up to 1000 capitate glands pe ... | 1989 | 2766700 |
the lack of correlation between the rate of rrna transport from nucleoli into cytoplasm and the duration of g1 and g2 phases in root meristem cells. | in root meristems of 3 species (secale cereale l., vicia faba l. subsp. minor, allium cepa l.) the durations of cell cycles and their phases were calculated using 3h-thymidine labelling. in the above species and in helianthus annuus l. (parameters of the cell cycle determined earlier) the g1 and g2 phase durations were different: g1 + 1/2 m from 3 h to 6.1 h, g2 + 1/2 m from 1.1. h to 8.3 h, depending on the species. the rate of rrna transport from nucleoli into cytoplasm during recovery after c ... | 1989 | 2767280 |
sequence and expression of a gene encoding an albumin storage protein in sunflower. | the complete sequence of a sunflower (helianthus annuus) gene, hag5, encoding a 2 s albumin storage protein was determined. the predicted unprocessed precursor has 295 amino acids, is rich in glutamine residues (24%) and contains a hydrophobic amino-terminus that is similar to the consensus signal peptide. amino acid sequencing of the mature protein revealed extensive post-translational processing. nuclease protection and primer extension analysis indicated a major transcriptional start 30 nucle ... | 1987 | 2830455 |
physical and gene organization of mitochondrial dna in fertile and male sterile sunflower. cms-associated alterations in structure and transcription of the atpa gene. | to study the molecular basis of cytoplasmic male sterility (cms) in sunflower (helianthus annuus), we compared the physical organization and transcriptional properties of mitochondrial dnas (mtdnas) from isonuclear fertile and cms lines. mapping studies revealed much greater similarity between the two mtdnas than in previous comparisons of fertile and cms lines from other plant species. the two sunflower mtdnas 1) are nearly identical in size (300 kb and 305 kb); 2) contain the same 12 kb recomb ... | 1988 | 2836801 |
cell lineage patterns in the shoot meristem of the sunflower embryo in the dry seed. | we mapped the fate of cells in the shoot meristem of the dry-seed embryo of sunflower, helianthus annuus l. cv. peredovic, using irradiation-induced somatic sectors. we analyzed 249 chlorophyll-deficient or glabrous (hairless) sectors generated in 236 plants. most sectors observed in the inflorescence extended into vegetative nodes. thus cell lineages that ultimately gave rise to reproductive structures also contributed to vegetative structures. no single sector extended the entire length of the ... | 1989 | 2909405 |
temperature and the cell cycle. | during the period between successive divisions, a cell traverses three stages of interphase: g1 (pre-synthetic interphase), s-phase (dna synthetic interphase) and g2 (post-synthetic interphase). the time taken for all cells in a meristem to divide (the cell doubling time (cdt] decreases in response to an increase in temperature. for example, the cdt in root meristems of zea mays decreases 21-fold as the temperature is increased from 3 to 25 degrees c. whether all phases of the cell cycle alter p ... | 1988 | 3077857 |
[use of sunflower (helianthus annus, l.) in human foods. i. preparation of sunflower flour, protein concentrate and supplementation of this flour with the amino acids lysine and methionine]. | in view of the importance that cultivation of sunflower (helianthus annus, l.) has from both the industrial and scientific points of view, to promote its wide utilization in human foods, this study was undertaken for the following purposes. to obtain an edible sunflower seed meal, as well as well to determine the effect thermic treatment exerted on its nutritional qualities. to evaluate through chemical and biological analyses, the nutritional quality of the flour and of its thermic treatment, a ... | 1988 | 3155292 |
monitoring the fate of dietary proteins in rumen fluid using gel electrophoresis. | 1. when fractionated by sodium dodecylsulphate-polyacrylamide gel electrophoresis (sds-page), strained rumen fluid from sheep fed on pelleted lucerne (medicago sativa) hay showed no major protein components that stain with coomassie blue. this feature made it possible to monitor the fate of individual polypeptides within a protein mixture incubated in rumen fluid in vitro. 2. extracts from a number of seed meals (sunflower (helianthus annuus), lupin (lupinus angustifolius), rape (brassica napus) ... | 1988 | 3196671 |
[nutritional potential of sunflower flour (helianthus annuus)]. | 1988 | 3272116 | |
transport of newly synthesized ribosome precedes initiation of s phase in root meristem cells in helianthus annuus l. | according to earlier results, cold treatment blocks most of cells in g1 phase; after 3 h of postincubation at 20 degrees c these cells initiate s phase. simultaneous cytophotometric (dna stained with methyl green) and autoradiographic (3h-uridine, 3h-arginine) analyses of cold pretreated cells have shown that transport of 3h-rna into cytoplasm is faster in g2 than in g1 cells: radioactivity of cytoplasm is faster in g2 cells becomes higher than that of nucleoli as early as between 2 h and 2.5 h ... | 1987 | 3450536 |
autoradiographic, ultrastructural and interferometric analyses of the temperature effect on the synthesis and migration of ribosomes in root meristem cells of helianthus annuus l. relation to s phase initiation. | experimental model consisted in blocking cells in g1 phase by cold treatment (12 h, 10 degrees c); following 3 h of postincubation at 20 degrees c, cells initiated s phase. in the present studies it has been shown that 2 h postincubation at 20 degrees c of cold-treated young seedlings of helianthus annuus l. results in transformation of inactive meristematic nucleoli, characterized by small sizes, reduced amount of dry mass and granular component and by the presence of few and large fibrillar ce ... | 1987 | 3622856 |
nucleotide sequence of a cdna encoding the ribulose-1,5-bisphosphate carboxylase/oxygenase from sunflower (helianthus annuus). | 1987 | 3822825 | |
proceedings: polyploidy and habituation in a long-term callus culture as compared to crown gall tissue in vivo (helianthus annuus l.). | 1974 | 4461472 | |
development and aging in helianthus annuus l. effects of the biological milieu of the apical meristem on patterns of development. | 1972 | 4653614 | |
fatty acid composition of sunflower (helianthus annus l.) oil as influenced by seed position. | 1973 | 4785175 | |
trypsin inhibitor content in some varieties of soya bean (glycine max l.) and sunflower seeds (helianthus annuus l.). | 1974 | 4859576 | |
[tumorigenic action of nucleic acid of a bacteriophage present in tumoral tissue cultures of sunflower (helianthus annus)]. | 1970 | 4987583 | |
biological quality of a lipoprotein concentrate from sunflower seed (helianthus annuus l.). | 1971 | 5127161 | |
the amino acid sequence of helianthus annuus l. (sunflower) cyrochrome c deduced from chymotryptic peptides. | peptides derived from digestion of 1 mumol of sunflower cytochrome c with chymotrypsin were separated by paper electrophoresis. the sequences of these peptides were determined by using the dansyl-edman method (gray & hartley, 1963) and confirmed by analysis of their amino acid composition. comparison of the set of peptides with the chymotryptic peptides of mung-bean (thompson, laycock, ramshaw & boulter, 1970) and wheat germ (stevens, glazer & smith, 1967) cytochrome c shows a clear homology. th ... | 1970 | 5500313 |
phenolic and sugar components of armavirec variety sunflower (helianthus annuus) seed meal. | 2013 | 5524462 | |
[rhizosphere products of helianthus annuus and spinacia oleracea]. | 1967 | 5584637 | |
[influence of d-leucine on helianthus annuus l]. | 1967 | 5584638 | |
factors affecting the development of the perithecial stage of powdery mildew of helianthus annuus l. in india. | 1965 | 5876680 | |
effect of ethylene on auxin transport. | ethylene was found to have no influence on auxin transport in hypocotyls of helianthus annuus and phaseolus vulgaris; coleoptiles of zea mays; petiole sections of gossypium hirsutum, phaseolus vulgaris, and coleus blumei. in the experiments described here, the tissues were treated with ethylene only during the 3 hours of polar transport. this short treatment is in contrast to the methods of others who found an effect of ethylene on auxin transport when plants grown in ethylene are used as experi ... | 1966 | 5990936 |
isolation and identification of trachyloban-19-oic and (-)-kaur-16-en-19-oic acids as antimicrobial agents from the prairie sunflower, helianthus annuus. | 1983 | 6361218 | |
structure and synthesis of histopine, a histidine derivative produced by crown gall tumors. | histopine, an unusual amino acid derivative of histidine isolated from crown gall tumors of sunflowers (helianthus annus) inoculated with agrobacterium tumefaciens strain b6, was previously assigned the gross structure n-(1-carboxyethyl) histidine (2). a diastereomeric mixture containing histopine (2a and 2b) was readily prepared by reductive alkylation of (s)-histidine (1) with pyruvic acid and sodium cyanoborohydride. the individual diastereomers were prepared by reaction of (s)-histidine with ... | 1984 | 6466642 |
copper deficiency in helianthus annuus l.: developmental and metabolic consequences, stomatal function and senescence. | the effects of copper deficiency on developmental morphology and on age related metabolic activity, water utilization, stomatal response to light and leaf senescence in the sunflower, helianthus annuus l., were monitored by sampling single leaves representing each leaf pair at designated harvest times during much of the vegetative lifespan. plants grown in nutrient solutions lacking copper exhibited no apparent abnormalities during early vegetative growth and leaf morphology differed little from ... | 1983 | 6618261 |
enhanced rrna transport from nucleoli into cytoplasm at g1/s, s/g2, and g2/m transitory points of the cell cycle in root meristem of helianthus annuus l. | results obtained by use of cytophotometric, autoradiographic and electron-microscopic methods indicate that periodic changes occur during the course of the cell cycle in synthesis, accumulation and transport of rrna. at the final periods of g1, s, and g2 phases an increased transport of rrna into cytoplasm takes place; owing to an abrupt loss of nucleolar contents a transparent zone ("vacuole") appears and the majority of nucleoli becomes visible as ring-shaped. due to an intensive synthesis and ... | 1984 | 6698032 |
the role of lysophosphatidylcholine in lipid synthesis by developing sunflower (helianthus annuus l.) seed microsomes. | the incorporation of oleate from oleoyl-coa into lipids by microsomes from developing sunflower (helianthus annuus l.) seeds has been investigated. oleate was incorporated mainly into position 2 of phosphatidylcholine or released as free fatty acid. the addition of exogenous 1-acyl-lysophosphatidylcholine increased the incorporation of oleate into position 2 of phosphatidylcholine and decreased the release of free oleate. in the absence of exogenous lysophosphatidylcholine, the incorporation of ... | 1984 | 6742852 |
the biosynthesis of triacylglycerols in microsomal preparations of developing cotyledons of sunflower (helianthus annuus l.). | the synthesis of triacylglycerols was investigated in microsomes (microsomal fractions) prepared from the developing cotyledons of sunflower (helianthus annuus). particular emphasis was placed on the mechanisms involved in controlling the c18- unsaturated-fatty-acid content of the oils. we have demonstrated that the microsomes were capable of: the transfer of oleate from acyl-coa to position 2 of sn-phosphatidylcholine for its subsequent desaturation and the return of the polyunsaturated product ... | 1984 | 6743281 |
the synthesis of acetylcholine by plants. | choline acetyltransferase was demonstrated in nettles (urtica dioica), peas (pisum sativum), spinach (spinacia oleracea), sunflower (helianthus annuus) and blue--green algae by using a sepharose--coash affinity column. the column effected a 1500-fold purification of the enzyme from nettle homogenates and was required for demonstrating activity in the other higher plants. demonstration of the enzyme in blue-green algae suggests that acetylcholine was a biochemical necessity in the earliest photos ... | 1981 | 6796060 |
tritiated water vapor exchange in sunflowers. | water vapor exchange between sunflowers (helianthus annuus l.) and the atmosphere was studied in darkness and in light using tho as a tracer. in the presence of tritiated water vapor, the exchange of tho measured as tritiated water in the leaves was slower in darkness than under illumination. this is explained by the variation of stomatal conductance. the authors show that a single curve is capable of summarizing the tritium exchange under any environmental condition, provided adequate units are ... | 1983 | 6885481 |
significance of plant metabolism in the mutagenicity and toxicity of pesticides. | in an attempt to gain an overall estimate for the influence of plant metabolism on environmental mutagenesis, the mutagenicity of an assortment of agricultural pesticide preparations in the salmonella bio-assay was evaluated using both rat liver s9 and plant enzyme homogenates as activating systems. the study indicates that plant metabolism can alter the results of this short-term mutagenicity test: some compounds which are non mutagenic in the salmonella bio-assay (e.g. diquat) give positive re ... | 1982 | 7172099 |
biological activities of sesquiterpene lactones from helianthus annuus: antimicrobial and cytotoxic properties; influence on dna, rna, and protein synthesis. | sesquiterpene lactones, produced in light and capable of inhibiting auxin-induced elongation growth of coleoptile and hypocotyl segments, were isolated from young leaves of helianthus annuus (spring and hager, planta in press, 1982). these compounds have an antibiotic effect on gram-negative and gram-positive bacteria as well as on some fungi. the minimal inhibiting concentration (mic) of compound ii (15-hydroxy-3-dehydrodesoxyfruticin, fig. 1), for example, is 15 micrograms/ml for bacillus brev ... | 1982 | 7183030 |
[obtaining flour and a protein concentrate from seeds of the helianthus annuus (sunflower) and its addition in cookies]. | the objectives of the studies described herein were: 1) to obtain a sunflower flour and protein concentrate for human consumption, and 2) to explore possible ways for their utilization. the peredovik variety was selected, since it is most abundant in mexico. the procedure usually followed for the obtention of flours for animal feeds was used with some modifications. the hull was mechanically eliminated with a yield of 51%, with a satisfactory appearance of the almond. in order to facilitate the ... | 1980 | 7283630 |
rhizosphere fungi of five plants in egypt. | 75 species which belong to 25 genera were collected during this investigation. all of these fungi were previously isolated from egyptian soils and other sources. the total count and the number of genera and species in the rhizosphere soil of the 5 plants were regularly higher than in non-rhizosphere soil. the composition of the rhizosphere fungus flora was considerably influenced by the type and the age of the plant. the most prevalent fungi in the rhizosphere of the test plants after 45, 90, an ... | 1980 | 7424047 |
pollen allergen homologues in barley and other crop species. | pollen from 10 agricultural plant species was surveyed for the presence of proteins crossreactive with group i, group iv and group ix allergens. barley (hordeum vulgare), maize (zea mays), rye (secale cerale), triticale (xtriticosecale cereale), oats (avena sativa), canola (brassica napus) and sunflower (helianthus annus) pollens contained numerous allergen cognate proteins. northern blot analysis of barley pollen rna revealed the presence of group i and group ix allergen transcripts. the barley ... | 1995 | 7537171 |
expression and in vitro targeting of a sunflower oleosin. | two distinct cdnas encoding oleosins (oil body proteins) have been identified by degenerate pcr as transcripts present in the developing seeds of sunflower (helianthus annuus l. cv. dwarf sunbred). one (psom) of these is closely related to a reported sunflower oleosin, whilst the other (pso5) has not been previously described. different expression patterns were observed for the two cdnas, pso5 being expressed earlier than psom in seed maturation and oil deposition. the results support the conten ... | 1995 | 7579191 |
isolation and partial characterization of allergens from helianthus annuus (sunflower) pollen. | we have purified four allergens from helianthus annuus (sunflower) pollen, hereafter named as allergens a, b, c, and d. under native conditions, allergen a has a mol. mass of 32, allergen b has one of 24, and allergens c and d each have one of 55 kda. at the least, allergens b, c, and d demonstrate charge heterogeneity, and the electrophoretic mobility of allergens c and d increases when these allergens are deglycosylated with trifluoromethanesulfonic acid. cross-reactivity among the four allerg ... | 1994 | 7709994 |
the effect of cd2+ on lipid components of sunflower (helianthus annuus l.) seeds. | the effect of seven levels of cd2+ viz. 0, 10, 20, 30, 40, 60 and 80 ppm (micrograms/g soil) supplied as cadmium chloride, on lipid components of sunflower seeds was studied in a pot experiment. the total and neutral lipids decreased while polar lipids (phospho and glycolipids) increased with the increasing levels of cd2+. oleic acid and linoleic acids were the major fatty acids in the total, polar and neutral lipid fractions. oleic acid generally increased while linoleic acid decreased in all t ... | 1995 | 7792263 |
immunogold labelling indicates high catalase concentrations in amorphous and crystalline inclusions of sunflower (helianthus annuus l.) peroxisomes. | immunogold labelling and electron microscopy were used to investigate whether catalase was present in peroxisomal inclusions, the composition of which has not yet been determined in plant cells. in the mesophyll cells of sunflower (helianthus annuus l.) cotyledons, the catalase gold label was confined to peroxisomes. at day 2 of postgerminative growth in darkness, peroxisomes were free of inclusions, and the matrix was homogeneously labelled with gold particles. thereafter, amorphous inclusions ... | 1995 | 7797423 |
nucleotide and deduced amino acid sequence of a putative higher molecular weight precursor for catalase in sunflower cotyledons. | a cdna from a sunflower (helianthus annuus l.) library encoded a 56.8 kda catalase peptide. n-terminal sequence comparisons to peroxisomal higher molecular weight precursors revealed conserved amino acid motifs around the (putative) cleavage sites. these findings suggest that the 55 kda catalase in sunflower cotyledons is synthesized at a higher molecular weight. | 1994 | 7803505 |
lipoxygenase activity on the plasmalemma of sunflower protoplasts and its modulation. | a lipoxygenase (ec 1.13.11.12) activity on the plasmalemma of sunflower (helianthus annuus l.) protoplasts and its possible modulation by regulatory molecules were investigated. the activity was followed as both linolenic acid-dependent conjugated diene formation and oxygen uptake. protoplasts exhibited a lipoxygenase activity inhibited by propyl gallate, salicylhydroxamic acid and butylated hydroxytoluene, and stimulated by the addition of cacl2, h2o2 and atp. the stimulation by cacl2 and h2o2 ... | 1995 | 7893738 |
sensitization to sunflower pollen: only an occupational allergy? | sunflower (helianthus annuus) pollen sensitization has been reported as an occupational allergy. in this report, the sensitization of the general population living in sunflower-growing areas to helianthus pollen was studied. both rast results in 32 adults with summer symptoms previously diagnosed as allergic to artemisia pollen, and cross-reactivity studies between h. annuus and other compositae suggested that h. annuus pollen was the main allergen involved in the hypersensitivity reaction of th ... | 1994 | 7920032 |
expression of a biologically active plant cytochrome b5 in escherichia coli. | cytochrome b5 from tobacco (nicotiana tabacum) was expressed in escherichia coli using a t7 polymerase/promoter system as described by studier, rosenberg, dunn and dubendorff (1990) (methods enzymol. 185, 60-89). transformed cells were red in colour and accumulated cytochrome b5 to a level of around 30% of the total cell protein. the purified cytochrome had oxidized, reduced and low-temperature absorbance spectra characteristic of plant microsomal cytochrome b5, and exhibited a c.d. spectrum res ... | 1994 | 7945268 |
nucleotide sequence of a pollen-specific cdna from helianthus annuus l. encoding a highly basic protein. | 1994 | 7972527 | |
selection and enrichment of differentially labeled plant protoplasts. | a fast and efficient method for the selection of plasmamembrane-marked protoplasts via magnetic-associated cell separation (macs) was developed. isolated mesophyll protoplasts from sunflower (helianthus annuus l.) were covalently labeled at their plasma membrane with fluoresceine isothiocyanate (fitc) with an efficiency of greater than 95%. they were subsequently coupled to specific monoclonal antibodies against fitc, which were conjugated to magnetic beads, and mixed with unlabeled hypocotyl pr ... | 1994 | 7989649 |
mitochondrial dna rflp and genetical studies of cytoplasmic male sterility in the sunflower (helianthus annuus). | fifteen sunflower (helianthus annuus l.) cytoplasmic male-sterile, and a single male-fertile, cytotypes were studied by both mtdna (mitochondrial dna) restriction fragment length polymorphism (rflp) and genetical analysis of male-fertility restoration patterns. it was found by multivariate analysis that the two methods of identification of cytoplasmic male sterility (cms) should be of use in sunflower breeding programs. the rflp study distinguished 13 groups based on differences in mtdna organiz ... | 1994 | 8001169 |
allergic contact dermatitis from sunflower (helianthus annuus) with cross-sensitivity to arnica. | 1993 | 8096447 | |
a microsatellite marker in helianthus annuus l. | the importance of genetic polymorphism detected by 'microsatellites' is now well established in mammalian genomes. sequences with a ca repeat, specific to sunflower, helianthus annuus l., were found by screening a genomic library in m13. after amplification, some polymorphism was detected on these sequences within a sample of cultivars and populations. | 1994 | 8111041 |
pattern of cross-sensitivity between 4 compositae plants, parthenium hysterophorus, xanthium strumarium, helianthus annuus and chrysanthemum coronarium, in indian patients. | to assess the pattern of cross-sensitivity between 4 members of the compositae family, namely parthenium hysterophorus l., xanthium strumarium l., helanthus annuus l. and chrysanthemum coronarium l., 63 patients clinically diagnosed to have airborne contact dermatitis, and 51 controls having well-defined patterns of contact dermatitis caused by agents other than plants, were patch tested with measured amounts of standardized aqueous extracts of these plants. positive reactions were obtained in 6 ... | 1994 | 8187516 |
[evaluation of the malate dehydrogenase activity in deteriorated sunflower seeds]. | malate dehydrogenase (mdh) activity in sunflower (helianthus annus l. cv. peredovik) seeds stored at 65 degrees c and 90 degrees c for 1, 3, 5, 7, and 14 days was evaluated. this study was design to discern the usefulness of this viability test for deteriorated seeds. differences in the resistance to the heat storage were detected between treatments as reflected by changes in the evolution of mdh activity. a decrease of mdh activity in both soluble and mitochondrial fractions was observed, which ... | 1993 | 8209098 |
the carboxyl terminus of isocitrate lyase is not essential for import into glyoxysomes in an in vitro system. | a procedure has been established for the isolation of intact and import-competent glyoxysomes from the cotyledons of sunflower (helianthus annuus) seedlings. radiolabeled isocitrate lyase (a glyoxysomal matrix protein) was produced by in vitro transcription of the castor bean cdna and translation of the rna in a wheat germ lysate. the heterologous isocitrate lyase was imported into isolated sunflower glyoxysomes and protease-protected. dihydrofolate reductase, a cytoplasmic protein, neither boun ... | 1993 | 8463266 |
chromosomal and genic barriers to introgression in helianthus. | the sexual transfer of genes between taxa possessing different structural karyotypes must involve the passage of genes through a chromosomal sterility barrier. yet little is known about the effects of structural differences on gene introgression within or adjacent to the rearranged chromosomal fragments or about the patterns of introgression in collinear regions. here, we employ 197 mapped molecular markers to study the effects of chromosomal structural differences on introgression in backcrosse ... | 1995 | 8582621 |
purification and characterization of oil-bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (helianthus annuus l.) | oil-bodies, from the immature cotyledons of sunflower (helianthus annuus l.), were difficult to purify to homogeneity using conventional techniques. the major protein contaminants were albumin and globulin storage proteins. a protocol has been developed, therefore, based upon the stringent washing of the oil-body fraction in 9 m urea, which effectively removed almost all the contaminating protein as judged by sds/page. the urea-washed oil-bodies were enriched in two major proteins of m(r) 19000 ... | 1996 | 8660304 |
growth of corn (zea mays) and sunflower (helianthus annuus) plants is affected by water and sludge from a sewage treatment plant. | 1996 | 8661913 | |
identification and mapping of trna genes on the helianthus annuus mitochondrial genome. | the physical map for seventeen trna genes on the mitochondrial genome of the dicotyledonous plant helianthus annuus has been established. eleven are genuine mitochondrial genes, while the other six show a high degree of similarity with the chloroplast counterparts. the genes, with the exception of the genuine trns(gct) and of the chloroplast-like trnv and trnp, are expressed. the comparison of the organization of some trna genes in the h. annuus mitochondrial genome with that of similar genes de ... | 1996 | 8722570 |
oil-bodies from sunflower (helianthus annuus l.) seeds. | 1996 | 8760388 | |
cytotoxicity of triterpenoid saponins. part 1: activities against tumor cells in vitro and hemolytical index. | genuine saponins from solidago virgaurea l., heteropappus altaicus (willd.) novopokr., h. biennis (ldb.) tamamsch., and helianthus annuus l. (asteraceae) as well as related carbohydrate modified glycosides of polygalacic acid and echinocystic acid were investigated in view of their structure related cytotoxicity in the yac-1- and in the p-815-tumor cell model. the cytotoxic activities are influenced by both, the o-glycosylation pattern at carbon atom 3 and 28 of the sapogenin. the ic50-values of ... | 1996 | 8767852 |
identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype. | we have studied two lines of sunflower (helianthus annuus l.) selected in the field as drought-tolerant (r1 genotype) or drought-sensitive (s1 genotype). when subjected to drought conditions, the r1 line was able to maintain high leaf water potential longer and wilted later than the s1 line. therefore, this indicates that r1 tolerance includes a leaf-adaptive response. by subtractive hybridization, we have isolated six different cdnas (designated sdi for sunflower drought-induced) corresponding ... | 1996 | 8806412 |
purification, characterization, and sequencing of two cysteine proteinase inhibitors, sca and scb, from sunflower (helianthus annuus) seeds. | two proteinaceous cysteine proteinase inhibitors (cystatins) referred to as sca and scb were purified to homogeneity from the seeds of sunflower (heliantas annuus) by gel filtration on sephadex g-75 followed by a series of ion-exchange column chromatographies and reverse-phase hplc (rp-hplc). the isoelectric points (pi) of sca and scb were estimated to be 5.6 and 9.6, respectively. the inhibitory potencies of these two cystatins were examined with cysteine proteinases from various sources, such ... | 1996 | 8827445 |
antimicrobial benzopyrans from the receptacle of sunflower. | the antimicrobial substances in sunflower (helianthus annuus) were investigated. two antifungal benzopyran derivatives, 6-acetyl-2,2-dimethyl-1,2-benzopyran (1) and 6-acetyl-7-hydroxy-2,2-dimethyl-1,2-benzopyran (2), were isolated from the ethanol extract of sunflower receptacles. the antimicrobial activities of isolated compounds 1 and 2 and their related compounds (precocenes) were evaluated by the paper disk method, using pyricularia oryzae as the test fungus. | 1996 | 8829537 |
influence of light on dna content of helianthus annuus linnaeus. | mean nuclear 2c dna content (c equaling haploid dna per nucleus) of the first leaf of the sunflower, helianthus annuus l., is influenced by the quality and the quantity of light. seedlings of two inbred lines, rha 299 and rha 271 were germinated and grown in controlled environmental conditions. lighting was adjusted to provide different combinations of photon flux densities and red to far red (r:fr) ratios. at r:fr = 5.8 and photon flux densities of 170 mumol.m-2.s-1, 200 mumol.m-2.s-1, and 230 ... | 1996 | 8855344 |
biochemical changes during regeneration of sunflower (helianthus annuus l.). | the main developmental stages in helianthus annuus organogenesis have been studied in the sunflower hybrid "giove". shoot regeneration was obtained with high efficiency from mature seed cotyledons. two-dimensional electrophoresis of protein extracts as well as the isozyme patterns of acid phosphatase, alcohol dehydrogenase, esterase, gluconate-6-phosphate dehydrogenase and phosphoglucomutase were compared during growth, callusing and regeneration. two-dimensional protein patterns were similar, a ... | 1996 | 8907539 |
triterpene alcohols from the flowers of compositae and their anti-inflammatory effects. | eleven tabular and nine ligulate flowers from 15 species of compositae plants were investigated for their triterpene alcohol constituents. this led to the isolation and identification of 11 triterpene alcohols as follows: heliaol, taraxasterol, psi-taraxasterol, alpha-amyrin, beta-amyrin, lupeol, taraxerol, cycloartenol, 24-methyl-enecycloartanol, tirucalla-7,24-dienol and dammaradienol. the tabular flowers of calendula officinalis, carthamus tinctorius, cosmos bipinnatus, chrysanthemum morifoli ... | 1996 | 8987908 |
cytophotometric determination of heterochromatin base composition in interphase nuclei of plant cells. | the use of dna base-specific fluorochromes and static cytophotometry at different thresholds of fluorescence intensity is proposed for studying the base composition of differently condensed chromatin fractions in plant interphase nuclei. results are reported of such analyses on nuclei from leaflets of posidonia oceanica and helianthus annuus, which confirm other findings obtained on the same materials by biochemical and molecular methods. | 1996 | 9116335 |
the predominant protein in peroxisomal cores of sunflower cotyledons is a catalase that differs in primary structure from the catalase in the peroxisomal matrix. | this paper describes a biochemical study on the protein composition of crystalline inclusions (cores) from plant peroxisomes. by sds/page and immunoblotting, a catalase of 59 kda was identified as the predominant protein component in purified cores from sunflower (helianthus annuus l.) cotyledons. a 55-kda catalase was the only additional peptide detected. in contrast to in cores, the 55-kda catalase was the major catalase protein in matrix fractions obtained from lysed peroxisomes. these findin ... | 1997 | 9151971 |
recombinant dna technology in allergology: cloning and expression of plant profilins. | profilin, an ubiquitous protein involved in eukaryotic cytoskeleton regulation, has been previously described as allergen in grasses, weeds and trees and in many fruits and vegetables, and it is in part responsible for cross-reactivities pollen and food allergic patients. completed cdna clones coding for phleum pratense, olea europaea, cynodon dactylon, parietaria judaica and helianthus annuus pollen profilins were isolated and sequenced. the deduced amino acid sequences share high identity with ... | 1997 | 9208049 |