Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| hydrolysis of aryl beta-maltotriosides by sweet potato beta-amylase and soybean beta-amylase. | sweet potato beta-amylase [ec 3.2.1.2, alpha 1,4-d-glucan maltohydrolase]-catalyzed hydrolyses of aryl beta-maltotriosides with substituents, no2-, cl-, and br- at the o-, m-, and p-positions in the phenyl ring were studied at ph 4.8 and 25 degrees c. the hydrolyses of a few of the maltotriosides by soybean beta-amylase [ec 3.2.1.2, alpha-1,4-d-glucan maltohydrolase] were also studied at ph 5.4 and 25 degrees c. it was found that the aryl beta-maltotriosides were preferentially hydrolyzed into m ... | 1998 | 147271 |
| a new method for the preparation of beta-amylase from sweet potato. | a simple method for the preparation of sweet potato beta-amylase by thymol amylose adsorption is described. the method is far more efficient and gives higher recovery of the enzyme. the crystalline enzyme thus obtained is found to be homogeneous by gel chromatography, polyacrylamide gel electrophoresis. | 2003 | 155814 |
| toxicity of novel sequiterpenoids from the stressed sweet potato (ipomoea batatas). | 2004 | 520970 | |
| evaluation of several methods for analysis of sweet potato phenolics. | 1967 | 546955 | |
| protein and amino acids of sweet potato (ipomoea batatas (l.) lam.) fractions. | 2008 | 659720 | |
| pig-bel--a zoonosis? | it is postulated that the combination of a very low protein diet, with low pancreatic tryptic activity, plus the continual consumption of sweet potato trypsin inhibitors leads to inadequate proteolysis when a meal containing meat contaminated with c. perfringens type c and its beta toxin is consumed. the necrotizing pathogenic process concluding as segmental gangrene is set in motion by toxin produced under altered dietary conditions first by motor paralysis via beta toxin, followed by villous a ... | 1978 | 731766 |
| synthesis and turnover of phenylalanine ammonia-lyase in root tissue of sweet potatoe injured by cutting. | 1. antibody toward phenylalanine ammonia-lyase of root tissue of sweet potato injured by cutting was obtained by immunizing of a rabbit with purified enzyme. 2. phenylalanine ammonia-lyase activity developed markedly in disks of sweet potato roots in response to cut injury, reached a maximum after 12 h, and then decreased thereafter. phenylalanine ammonia-lyase content, which was determined by the quantitative immunoprecipitation method, changed in parallel with enzyme activity. the rate of inco ... | 1977 | 837940 |
| bacterial stalk rot of maize, its symptoms and host-range. | stalk rot of maize, caused by erwinia carotovora f. sp. zeae sabet (re-designated as pectobacterium chrysanthemi pathovar. zeae by kelman 1974) showed first premature withering and drying up of the uppermost leaves which was soon followed by the lower leaves. the rot either extended from the base upwards (basal rot) or from the top downwards (top rot). in the case of basal rot, the leaves become yellow and the infected tissue becomes brown, soft, and water soaked. internally, the stalk turns int ... | 1977 | 857508 |
| biogenesis of lung-toxic furans produced during microbial infection of sweet potatoes (ipomoea batatas). | 1977 | 864134 | |
| immunochemical studies on fluctuation of phenylalanine ammonia-lyase activity in sweet potato in response to cut injury. | antibody toward phenylalanine ammonia-lyase (pal) [ec 4.3.1.5] was obtained by immunization of a rabbit with highly purified pal. the antibody reacted specifically with ral, as demonstrated by the ouchterlony double diffusion test, immunoelectrophoresis, and sds polyacrylamide gel electrophoresis of the immunoprecipitate. experiments using anti-pal showed that pal was not present in fresh sweet potato tissue, but appeared in response to cut injury, reaching a maximum, and then decreasing, in par ... | 1976 | 939758 |
| the detection and quantitative determination of ipomeamarone in damaged sweet potatoes (ipomoea batatas). | 2009 | 1133296 | |
| toxigenic fungi in food. | forty-five fungal isolates from moldy supermarket foods were tested for toxicity to brine shrimp, and twenty-two of these isolates were subsequently tested for toxicity to chicken embryos. highly toxigenic fungi were cladosporium sphaerospermum from a bakery product, fusarium oxysporum from carrots, f. solani from cabbage, aspergillus niger and penicillium corylophilum from bread, p. cyclopium and p. herguei from corn meal, p. lanosum from onions,p. steckii from chocolate syrup, penicillium sp. ... | 1975 | 1147614 |
| purification and properties of sweet potato mild mottle, a white-fly borne virus from sweet potato (ipomoea batatas) in east africa. | 1976 | 1267233 | |
| resin glycosides. xv. simonins i-v, ether-soluble resin glycosides (jalapins) from the roots of ipomoea batatas (cv. simon). | five new ether-soluble resin glycosides (jalapins), simonins i-v, have been isolated from the roots of ipomoea batatas and characterized on the bases of chemical and spectral data. simonin i is the first example of resin glycoside with aromatic acid (trans-cinnamic acid) as a component organic acid. | 1992 | 1294318 |
| construction of hybridomas secreting monoclonal antibody against sweet potato feathery mottle virus and use of antibody for detection of spfmv. | two hybridomas stably secreting monoclonal antibodies (mcab) against sweet potato feathery mottle virus (spfmv) were constructed by fusing mouse myeloma (sp2/0-ag14) with mouse splenocytes immunized with spfmv-lf. the mcab secreted by the two hybridomas specifically reacted with both spfmv-lf (china strain) and spfmv-rc (american strain) in dot-blot-elisa. a large amount of mcab was produced with balb/c mice. the titer of hybridoma cultured supernatant was 1:256, and that of the ascitic fluids w ... | 1992 | 1343832 |
| isolation and primary structure of proteinase inhibitors from erythrina variegata (linn.) var. orientalis seeds. | the kunitz-type trypsin inhibitors, etia and etib, and chymotrypsin inhibitor eci were isolated from the seeds of erythrina variegata. the proteins were extracted from a defatted meal of seeds with 10 mm phosphate buffer, ph 7.2, containing 0.15 m nacl, and purified by deae-cellulose and q-sepharose column chromatographies. the stoichiometry of trypsin inhibitors with trypsin was estimated to be 1:1, while that of chymotrypsin inhibitor with chymotrypsin was 1:2, judging from the titration patte ... | 1992 | 1369077 |
| a nuclear gene encoding beta-amylase of sweet potato. | a nuclear amyb gene from sweet potato encoding beta-amylase (beta amy) that is abundant in tuberous roots and inducible in other organs by an exogenous supply of sucrose or polygalacturonic acid, was isolated and characterized. genomic southern blot hybridization, restriction maps of independently isolated phage lambda genomic clones, and the nucleotide sequence of amyb compared with that of the cdna, all suggested that beta amy of sweet potato is encoded by a gene that is present in a single co ... | 1992 | 1383095 |
| a simple, rapid method for purification of epsilon-subunit, coupling factor 6, subunit d, and subunit e from rat liver h(+)-atp synthase and determination of the complete amino acid sequence of epsilon-subunit. | the rat liver mitochondrial epsilon-subunit, coupling factor 6, subunit d, and subunit e of h(+)-atp synthase, which are all extra subunits with no counterparts in escherichia coli, were purified by reverse-phase high performance liquid chromatography. the complete amino acid sequence of the rat epsilon-subunit was determined by automated edman degradation of the whole protein and derived peptides. the protein contains 50 amino acids and has a molecular mass of 5635 kda. it is a basic hydrophili ... | 1992 | 1429613 |
| developmental changes induced by gamma irradiation in ipomoea batatas l. lam (sweet potato). | storage roots of ipomoea batatas l. lam were exposed to gamma irradiation at 90, 180, and 240 gy. the two highest doses caused a delay in the initiation of rooting and especially of shooting; they also caused formation of rosette-like, stemless shoots without apical meristems. the storage roots irradiated with 90 gy did form stems, but 50% of them had no apical meristem. the irradiated storage roots produced absorbent roots which developed shoots after 70 days of growth. nonirradiated storage ro ... | 1992 | 1438705 |
| maternity and medicinal plants in vanuatu. i. the cycle of reproduction. | ethnobotanical data collection to select pharmacologically active species was carried out within a clearly defined therapeutic context: those plants used during the course of a woman's reproductive life. extensive bibliographical and field data collection and cross-examination of the information thus gathered have provided us with a clearer picture of the effectiveness of these plant species. various concepts, behaviours and practices relating to menstruation, pregnancy, birth and birth control ... | 1992 | 1453707 |
| the nuclear factor sp8bf binds to the 5'-upstream regions of three different genes coding for major proteins of sweet potato tuberous roots. | sporamin and beta-amylase are two major proteins of tuberous roots of sweet potato, and expression of genes coding for sporamin and beta-amylase is induced concomitantly in leaves with the petioles attached by exogenous supply of sucrose or polygalacturonic acid. we have used a dnase i footprinting assay to characterize nuclear factors that bind to the 5'-upstream regions of gspo-a1, gspo-b1 and g beta-amy genes that encode a-type sporamin, b-type sporamin and the subunit of beta-amylase, respec ... | 1992 | 1531033 |
| dietary intake of vitamin a in african pre-school children in umlazi, south africa. | vitamin a deficiency has been shown to have an impact on morbidity and mortality and therefore prevalence rates are important for public health policy. we report the use of a 7-day recall, dietary questionnaire, which was validated and administered to 203 randomly selected urban african pre-school children in umlazi, south africa. common vitamin a containing foods eaten were leafy green vegetables, pumpkin, sweet potato, mangoes and chicken liver. although the mean daily intake of vitamin a was ... | 1992 | 1587704 |
| dna relatedness among strains of the sweet potato pathogen streptomyces ipomoea (person and martin 1940) waksman and henrici 1948. | dna relatedness among 28 putative strains of streptomyces ipomoea from geographically diverse locations and the type strain, nrrl b-12321, was determined spectrophotometrically. the data confirm that these 28 strains are not closely related genetically to the plant-pathogenic species streptomyces scabies (39% dna relatedness) or streptomyces acidiscabies (17% dna relatedness) or any other major blue-spored streptomyces species (less than 30% dna relatedness). of the 29 strains examined, 4 could ... | 1992 | 1610178 |
| dna-dna hybridization in the systematics of streptomyces. | the dna relatedness among strains in several different phenotypically defined streptomyces species clusters was evaluated. it was found that the data from dna-relatedness studies do not necessarily agree with the clustering generated using numerical taxonomic techniques. a study of the morphologically heterogeneous 's. cyaneus' cluster showed that morphological criteria traditionally used to classify and identify streptomyces species still have value, since strains in dna-relatedness cluster gro ... | 1992 | 1612444 |
| acute interstitial pneumonia in cattle associated with consumption of mould-damaged sweet potatoes (ipomoea batatas). | 1992 | 1632730 | |
| the effect of diet on microfaunal population and function in the caecum of a subterranean naked mole-rat, heterocephalus glaber. | the effect of dietary fibre and starch content on digestibility, microfaunal population and caecal function was investigated in a subterranean mole-rat, heterocephalus glaber (rodentia). mole-rats were fed on a diet of either sweet potato (neutral-detergent fibre (ndf) 65 g/kg dry matter (dm), starch 638 g/kg dm) or carrot (ndf 157 g/kg dm, starch 258.7 g/kg dm) for 4 weeks. daily intake and faecal output were monitored. thereafter caecal microfaunal population, density and function were assesse ... | 1991 | 1645994 |
| [chemical constituents of ipomola batatas lam]. | three chemical compounds isolated from the leaves and stems of ipomoea batatas were identified as fumaric acid, succinic acid and 7,3',4'-trimethylquercetin by chemical and spectroscopic analysis. in this paper, an analysis of the amino-acids of ipomoea batatas is also reported. | 1991 | 1804202 |
| studies on aldose reductase inhibitors from natural products. iv. constituents and aldose reductase inhibitory effect of chrysanthemum morifolium, bixa orellana and ipomoea batatas. | the hot water extracts of chrysanthemum morifolium, bixa orellana and ipomoea batatas, were found to have potent inhibitory activity towards lens aldose reductase (ar). ellagic acid (4) was isolated from c. morifolium and i. batatas, isoscutellarein (7) from b. orellana and 3,5-dicaffeoylquinic acid (10) from i. batatas, respectively, as potent inhibitors. | 1991 | 1814628 |
| kinetics and mathematical model of ethanol formation by immobilized yeast cells. | kinetics of the ethanol fermentation with the immobilized k yeast cells was studied both experimentally and theoretically. a kinetic model consisting of 6 equations was proposed. in this model the combined inhibition, i.e., glucose in uncompetitive inhibition and ethanol in noncompetitive inhibition with irreversible performance, was taken into consideration. calculated values agreed approximately with the experimental results, and this model was found to be of practical use. the maximum average ... | 1991 | 1823593 |
| molecular cloning and expression in escherichia coli of cdna encoding the subunit of sweet potato beta-amylase. | tuberous roots of the sweet potato are unusually rich in beta-amylase, and the beta-amylase polypeptides account for about 5% of the total soluble protein of the organ. unlike beta-amylases from other origins, the sweet potato beta-amylase is a tetramer of identical subunits, and it also bears starch phosphorylase-inhibitor activity. a cdna for the subunit of sweet potato beta-amylase was obtained by immunological screening of an expression cdna library constructed by the vector-primer and linke ... | 1991 | 1837016 |
| ubiquinol-cytochrome c oxidoreductase of higher plants. isolation and characterization of the bc1 complex from potato tuber mitochondria. | a procedure is described for isolation of active ubiquinol-cytochrome c oxidoreductase (bc1 complex) from potato tuber mitochondria using dodecyl maltoside extraction and ion exchange chromatography. the same procedure works well with mitochondria from red beet and sweet potato. the potato complex has at least 10 subunits resolvable by gel electrophoresis in the presence of dodecyl sulfate. the fifth subunit carries covalently bound heme. the two largest ("core") subunits either show heterogenei ... | 1991 | 1851164 |
| purification and characterization of p-coumaroyl-d-glucose hydroxylase of sweet potato (ipomoea batatas) roots. | p-coumaroyl-d-glucose hydroxylase in sweet potato (ipomoea batatas lam.) has been purified to apparent electrophoretic homogeneity using a combination of anion-and cation-exchange, hydrophobic and gel filtration chromatography. the purified enzyme was a monomer with a molecular weight of 33,000 and pi of 8.3. the purified enzyme showed not only hydroxylase activity but also polyphenol oxidase activity. l-ascorbic acid was the best electron donor for the hydroxylation reaction, which had an optim ... | 1991 | 1989492 |
| propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting. | sporamin is a protein without glycans that accumulates in large quantities in the vacuoles of the tuberous root of the sweet potato. it is synthesized as a prepro precursor with an n-terminal extension composed of a 21-amino-acid signal peptide and a 16-amino-acid propeptide. a total of 48 base pairs, corresponding to the nucleotide sequence that encodes the propeptide, was deleted from a cdna clone for sporamin. this delta pro mutant sequence, as well as the sequence of the wild-type sporamin c ... | 1991 | 1992474 |
| high-level expression of a sweet potato sporamin gene promoter: beta-glucuronidase (gus) fusion gene in the stems of transgenic tobacco plants is conferred by multiple cell type-specific regulatory elements. | genes coding for sporamin, the most abundant protein of the tuberous root of the sweet potato, are expressed at a high levels in the stems of plantlets cultured axenically on sucrose-containing medium. their expression is also induced in leaf-petiole explants by high concentrations of sucrose. a fusion gene comprising of the 1 kb 5' upstream region of the gspo-a1 gene coding for the a-type sporamin and the coding sequence of bacterial beta-glucuronidase (gus) was introduced into the tobacco geno ... | 1991 | 2017135 |
| high-level expression of tuberous root storage protein genes of sweet potato in stems of plantlets grown in vitro on sucrose medium. | sporamin, the tuberous root storage protein of the sweet potato, accounts for about 60 to 80% of the total soluble protein of this organ. the amount of sporamin present in other organs is very low, or even not detectable, in the normal field-grown plants. however, the stem of sweet potato plantlets grown axenically on agar medium containing sucrose was found to accumulate large amounts of sporamin. two-dimensional gel electrophoretic profiles of sporamin precursors synthesized in vitro by poly(a ... | 1990 | 2102838 |
| the plant mitochondrial f1-atpase. the identity of the delta' (20 kda) subunit. | the n-terminal amino acid sequence of the 20 kda (delta') subunit of the turnip (brassica napus l.) mitochondrial f1-atpase has been determined. comparison of the sequence obtained with those of the epsilon subunits of chloroplast cf1, e. coli f1 and the delta subunit of bovine f1 shows that the turnip delta' subunit is another member of this family of homologous proteins. the delta' subunit of sweet potato f1-atpase [(1989) j. biol. chem. 264, 3183-3186] is very similar to the turnip sequence a ... | 1990 | 2147662 |
| alteration of the properties of aspergillus sp. k-27 glucoamylase on limited proteolysis with subtilisin. | an active derivative (mol. wt. 48,000) of aspergillus sp. k-27 glucoamylase (mol. wt. 76,000) was obtained by limited proteolysis with subtilisin. the amino acid sequences of native and modified enzymes at the n-termini were ala-gly-gly-thr-leu-asp and ala-val-leu, respectively. the proteolysis greatly decreased the affinity of the enzyme for amylopectin and glycogen, but not for oligosaccharides. it also reduced the ability of the enzyme to degrade raw starch, abolished the ability of the enzym ... | 1990 | 2224899 |
| vacuolar targeting and posttranslational processing of the precursor to the sweet potato tuberous root storage protein in heterologous plant cells. | sporamin, the tuberous root storage protein of the sweet potato, which is localized in vacuoles, is synthesized as a prepro-precursor with an n-terminal sequence of amino acids that includes a signal peptide and an additional pro-segment of 16 amino acids. a full-length cdna for sporamin was placed downstream of the 35 s promoter of cauliflower mosaic virus and introduced into tobacco and sunflower genomes by ti plasmid-mediated transformation. a polypeptide of nearly the same size as mature spo ... | 1990 | 2246259 |
| characterization of a cdna encoding cottonseed catalase. | a 1.7 kb cdna clone was isolated from our lambda gt11 library constructed from poly(a) rna of 24-h-old cotyledons. the cdna encodes a full-length catalase peptide (492 amino acid residues). the calculated molecular mass is 56,800, similar to that determined for purified enzyme (57,000 sds-page). among higher plant catalases, this cotton catalase shows the highest amino acid sequence identity (85%) to the subunit of homotetrameric maize cat 1, a developmental counterpart to the homotetrameric cat ... | 1990 | 2364113 |
| characterization of catalase transcripts and their differential expression in maize. | in maize, the three unlinked catalase (ec 1.11.1.6) structural genes (cat1, cat2 and cat3) are differentially expressed temporally, spatially and in response to environmental signals in the developing seedling. in order to understand more fully the molecular mechanisms involved in catalase gene expression, full-length cdna clones representing the maize cat1, cat2 and cat3 transcripts were isolated and characterized. dna sequence analysis confirmed that each cdna encodes a unique catalase protein ... | 1988 | 2461221 |
| possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group. | there are at least ten viruses identified in the literature that resemble definitive potyviruses in having flexuous filamentous particles and inducing the formation of "pinwheel" cytoplasmic inclusions in infected cells but that are transmitted by eriophyid mites, whiteflies or soil fungi and not by aphids, the vectors of the definitive potyviruses. the taxonomic status of these viruses is uncertain at present. using a broadly cross-reactive antiserum raised against the dissociated coat protein ... | 1989 | 2473719 |
| correspondence of minor subunits of plant mitochondrial f1atpase to f1f0atpase subunits of other organisms. | in addition to two major alpha- and beta-subunits, the soluble oligomycin-insensitive f1atpase purified from sweet potato root mitochondria contains four different minor subunits of gamma (mr = 35,500), delta (mr = 27,000), delta' (mr = 23,000), and epsilon (mr = 12,000) (iwasaki, y., and asashi, t. (1983) arch. biochem. biophys. 227, 164-173). among these minor subunits, the delta-subunit specifically cross-reacted with an antibody against the delta-subunit of maize mitochondrial f1 which conta ... | 1989 | 2536736 |
| expression and processing of cyanobacterial mn-stabilizing protein in escherichia coli. | the woxa gene of cyanobacterium anacystis nidulans r2, which encodes the precursor of the mn-stabilizing protein involved in photosynthetic water oxidation, was found to be expressed in escherichia coli. the 30-kda expression product was indistinguishable from the authentic mature protein on sds/page. upon fractionation of e. coli cells, the expression product was co-precipitated with the membrane fraction, which is consistent with the water-insoluble nature of the authentic mature protein. anal ... | 1989 | 2689172 |
| partial cleavage of sweet potato feathery mottle virus coat protein subunit by an enzyme in extracts of infected symptomless leaves. | the coat protein of particles of sweet potato feathery mottle potyvirus (spfmv) extracted from ipomoea spp. migrated in sds-page mainly as bands of m(r) 38,000 (38k), 36k, 32k, 30k. trypsin treatment of the particles resulted in the appearance of only one 30k polypeptide. the inclusion of protease inhibitors in the extraction procedure did not alter the heterogeneity of spfmv coat protein. a partially purified fraction of extracts from recovering, symptomless, but not from healthy leaves of i. n ... | 1989 | 2769226 |
| parotid gland enlargement and female reproductive performance in a papua new guinea highland population. | swelling of the parotid salivary glands is commonly observed among males and females of highland papua new guinea. evidence suggests that the condition is related to the highlanders' high starch consumption in the form of sweet potato. in this paper we regard parotid enlargement as an indicator of high energy intake and test its association with two measures of reproductive performance, the number of live births and living offspring, in a sample of 274 women from lufa in the eastern highlands di ... | 1989 | 2802523 |
| separation, amino-terminal sequence and cell-free synthesis of the smallest subunit of sweet potato cytochrome c oxidase. | the smallest subunit (v) of sweet potato cytochrome c oxidase was separated into three polypeptides, va, vb and vc with different molecular masses (7.4 kda, 6.8 kda and 6.2 kda respectively) by highly resolving sodium dodecylsulfate polyacrylamide gel electrophoresis. antibody against subunit v reacted specifically with the polypeptide vc. when polyadenylated mrna from sweet potato root tissue was translated in a wheat germ cell-free system, the smallest subunit (vc) of the polypeptides was synt ... | 2010 | 2885192 |
| molecular cloning and nucleotide sequence of full-length cdna for sweet potato catalase mrna. | a nearly full-length cdna clone for catalase (pcas01) was obtained through immunological screening of cdna expression library constructed from size-fractionated poly(a)-rich rna of wounded sweet potato tuberous roots by escherichia coli expression vector-primed cdna synthesis. two additional catalase cdna clones (pcas10 and pcas13), which contained cdna inserts slightly longer than that of pcas01 at their 5'-termini, were identified by colony hybridization of another cdna library. those three ca ... | 1987 | 2885193 |
| cf1atpase beta- and epsilon-subunit genes are separated in the sweet potato chloroplast genome. | 2010 | 2889184 | |
| processing of a plant vacuolar protein precursor in vitro. | a precursor for sporamin a, the storage protein of the tuberous roots of sweet potato deposited in the vacuole, is synthesized on membrane-bound polysomes and has an extra peptide of 37 amino acids at the n-terminus of the mature form, which can be divided into an n-terminal putative signal peptide sequence (residues -37 to -17) and a segment enriched with charged amino acids (residues -16 to -1) [hattori, t., et al. (1985) plant mol. biol. 5, 313-320]. we examined the in vitro processing of the ... | 1987 | 3301345 |
| quality improvement of sweet-potato (ipomoea batatas l. lam.) roots as feed by ensilage. | 1. sweet-potato (ipomoea batatas l. lam.) strips (sps) mixed with maize powder (cp) in proportions 10:0, 9:1, 8:2, and 7:3 were ensiled for 1, 2 or 3 months. 2. trypsin inhibitor activity (tia) decreased during ensilage in samples of all treatments while the sps-cp mixture (7:3, w/w) ensiled for 3 months contained the lowest tia. 3. sps-cp (8:2, w/w) dried or ensiled for 2 months, or ensiled for 2 months and dried, were each mixed with twice the amount of control diet (1:2, w/w) to make three di ... | 1988 | 3408700 |
| protease production with sweet potato residue by solid state fermentation. | the sweet potato residue being at the initial moisture content 50-58%, initial ph 3.5-4.3, supplemented with rice bran, and minerals, and incubated at 20-26 degrees c for 5 days was the optimal conditions for protease production with aspergillus niger ntu-am-1 by solid state fermentation. protease could be recovered by shaking at room temperature for one hour and extracted with five times volume of 0.1% nacl solution. the yield of protease was 814 units per gram dry weight of substrate. partiall ... | 1986 | 3545704 |
| [study of red color development of sweet potato epidermis by treatment with phosphate preparations]. | 2012 | 3567432 | |
| purification and characterization of hydroxycinnamoyl d-glucose. quinate hydroxycinnamoyl transferase in the root of sweet potato, ipomoea batatas lam. | we have previously proposed a chlorogenic acid biosynthetic pathway which involves a transesterification reaction between hydroxycinnamoyl d-glucose and d-quinic acid. the proposed pathway was based on tracer experimental results (kojima, m., and uritani, i. (1972) plant cell physiol. 13, 311-319). the enzyme that catalyzes the above reaction has been purified 160-fold from sweet potato root (ipomoea batatas lam.) and characterized. the purified enzyme yielded one band of 26,000 daltons on sodiu ... | 1986 | 3722170 |
| childhood malnutrition in the western province of the solomon islands. | cross-sectional anthropometric studies conducted in the western province of the solomon islands indicate that over half the children become malnourished before the age of 2 years, regardless of the nutritional indicator (weight for age, weight for height, height for age) used. in comparison to reference children, weight gain slows from the age of 6 months and mean weight is static from 9-17 months. stunting is more important than wasting. dietary research suggests that the principle cause of thi ... | 2013 | 3765099 |
| effects of commonly consumed vegetables on hepatic xenobiotic-metabolizing enzymes in the mouse. | to investigate the effects of consuming a variety of common vegetables on the activities of xenobiotic-metabolizing enzymes in the mouse liver, male c57bl/6 mice were fed purified diets supplemented isocalorically with 20% freeze-dried powdered kidney bean, soya bean, alfalfa, cauliflower, mustard greens, carrot, kale, brussels sprouts, beet, egg plant or onion or with 40% sweet potato. hepatic ethoxycoumarin o-deethylase (ecd) activity was increased significantly (p less than or equal to 0.05) ... | 1985 | 3877667 |
| studies of nitrogen balance in male highlanders in papua new guinea. | nitrogen metabolism in papua new guinea highlanders was studied by examining their nitrogen balance when they ate their usual diet (experiment 1) or an experimental diet (experiment 2). studies were made on 39 male highlanders between october and december in 1980 and 1982. in experiment 1, the average protein and energy intakes (mean +/- sd) for three consecutive days of usual diets were 116.2 +/- 31.2 mg n/kg body weight and 46.9 +/- 8.9 kcal/kg body weight in 1980 and 143.5 +/- 54.8 mg n/kg bo ... | 2014 | 3958801 |
| the nephrotoxicity for mice of deisopropylngaione, a minor furanoid component of toxic myoporaceous essential oils. | deisopropylngaione (din) is one of a family of hepatotoxic furanosesquiterpenoid essential oils which is found in small amounts (5%) in the leaves of some specimens of the australian plant myoporum deserti. din differs from other furanoid myoporaceous essential oils in that it also causes lesions in the lungs and kidneys. at the near ld50 dose rate of 150 mg kg-1 given by intraperitoneal injection, din is able to cause lethal renal proximal tubular necrosis without causing significant injury to ... | 1985 | 3989217 |
| effect of gossypol on some oxidative respiratory enzymes. | gossypol was examined in relation to its effect on certain enzymes and enzyme complexes associated with the tricarboxylic acid cycle and the electron transport system. succinic dehydrogenase and cytochrome oxidase activity from sweet potato was completely inhibited by gossypol at 7.5 x 10(-3)m and 2.0 x 10(-3)m, respectively. succinoxidase activity of the same preparations was fully inhibited at a lower concentration, 2.5 x 10(-4)m. this concentration did not affect either succinic dehydrogenase ... | 1966 | 4287149 |
| lung-toxic furanoterpenoids produced by sweet potatoes (ipomoea batatas) following microbial infection. | 1974 | 4373055 | |
| confirmation by chemical synthesis of the structure of 4-ipomeanol, a lung-toxic metabolite of the sweet potato, ipomoea batatas. | 1972 | 4502825 | |
| effect of triton x-100 on sweet potato -amylase. | 1972 | 4622930 | |
| a lung oedema factor from mouldy sweet potatoes (ipomoea batatas). | 2015 | 4930474 | |
| isolation and characterization of 4-ipomeanol, a lung-toxic furanoterpenoid produced by sweet potatoes (ipomoea batatas). | 2008 | 5016626 | |
| distribution of nitrogen and composition of nitrogen compounds in food, urine and faeces in habitual consumers of sweet potato and taro. | 1972 | 5068464 | |
| the action of sweet potato -amylase on glycogen and amylopectin: formation of a novel limit dextrin. | 1971 | 5165519 | |
| etiology of caries in papua-new guinea. associations in soil, food and water. | mineral analyses of garden soils, vegetables, and water samples from 21 villages in the sepik and fly river regions of the territory of papua and new guinea, in which the mean prevalence of dental caries ranged from 0 to 29.5% decayed teeth per person, have shown the following relationships between variations in the caries prevalence and the amounts of certain elements:(1) a strong inverse association with concentrations of alkali and alkaline earth elements, especially strontium, barium, potass ... | 1970 | 5314012 |
| oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury. | arrhenius plots of the respiration rates of mitochondria isolated from chilling sensitive plant tissues (tomato and cucumber fruit, and sweet potato roots) showed a linear decrease from 25 c to about 9 to 12 c (with q(10) values of 1.3 to 1.6), at which point there was a marked deviation with an increased slope as temperatures were reduced to 1.5 c (q(10) of 2.2 to 6.3). the log of the respiration rate of mitochondria from chilling resistant tissues (cauliflower buds, potato tubers, and beet roo ... | 1970 | 5427108 |
| toxicity of mould-damaged sweet potatoes (ipomoea batatas). | 1970 | 5428477 | |
| isolation of furanoterpene-containing particles from ceratocystis fimbriata-infected sweet potato root tissue. | furanoterpene-containing particles were isolated by centrifugation on a discontinuous ficoll density gradient from a homogenate of the non-infected tissue adjacent to the infected region of ceratocystis fimbriata-infected sweet potato root tissue. the particles were recovered at a relatively high ratio in the 2% ficoll fraction, in which there was no contamination by mitochondria and only little by endoplasmic reticulum judging from the distribution of the activities of their marker enzymes and ... | 1984 | 6099359 |
| partial purification and characterization of udpg:t-cinnamate glucosyltransferase in the root of sweet potato, ipomoea batatas lam. | previously, we isolated t-cinnamoyl-d-glucose as a possible intermediate in chlorogenic acid biosynthesis from sweet potato root. the enzyme which catalyzes the formation of t-cinnamoyl-d-glucose has been purified 539-fold from sweet potato root (ipomoea batatas lam.) and characterized. it required udp-glucose as a glucosyl donor. its molecular weight was estimated to be 45,000 by gel filtration chromatography through sephadex g-100. its km values were 0.2 mm for t-cinnamic acid and 0.1 mm for u ... | 1984 | 6231280 |
| inhibition of electron transport and oxidative phosphorylation in plant mitochondria by gladiolic acid and structurally-related aromatic ortho dialdehydes. | gladiolic acid (ga, 4-methoxy-5-methyl-0-phthalaldehyde-3-carboxylic acid), an antifungal aromatic ortho dialdehyde produced by penicillium gladioli was found to be a potent inhibitor of electron transport and oxidative phosphorylation reactions in sweet potato and mung bean mitochondria. similar results were also found with the naturally occurring ortho dialdehydes, cyclopaldic acid, quadrilineatin, and flavipin as well as the synthetic dialdehydes, 3-formyl opianic acid and 0-phthalaldehyde. b ... | 1980 | 6248181 |
| experimental pigbel: the production and pathology of necrotizing enteritis due to clostridium welchii type c in the guinea-pig. | an animal model for pigbel in man was developed using guinea-pigs. intragastric dosing with growing cultures of clostridium welchii type c only produced necrotic lesions if protease inhibitors were given as well. beta toxin, which is made by the type c organism, causes the intestinal damage and is very easily destroyed by proteases. protease inhibitors in soybean and aprotinin were effectively in inducing disease in animals on a normal diet, while inhibitors in sweet potato, which inhibit only t ... | 1980 | 6252934 |
| cholesterol binding capacity of fiber from tropical fruits and vegetables. | the cholesterol binding capacity of 28 fiber samples from a variety of the more common tropical fruits and vegetables was determined. the binding capacity of cholestyramine, cellulose, lignin, guar gum and citrus pectin were also determined. capacities were evaluated by an in vitro method that simulates the effect of the human digestive system on fiber using a series of enzymatic treatments before the binding was determined. binding values varied from 3% for a soluble fraction of cassava to 84% ... | 1984 | 6323908 |
| protein nutritional value of sweet potato flour. | 1983 | 6415140 | |
| solubilization of udp-glucose-specific starch synthetase in sweet-potato (ipomoea batatas) starch granules by the urea/pullulanase method. | the adp-glucose- or udp-glucose-specific starch synthetase bound to sweet-potato (ipomoea batatas) starch granules is localized in the granules, and the udp-glucose-specific enzyme was solubulized by urea/pullulanase treatment of the starch granules. | 1980 | 6450591 |
| utilization of fodder yeast and agro-industrial by-products in production of spores and biologically - active endotoxins from bacillus thuringiensis. | a number of newly-devised fermentation media were evaluated with respect to their ability to support sporulation and biosynthesis of endotoxins by strains of bacillus thuringiensis that are biologically active against spodoptera littoralis, heliothis armigera, and spodoptera exigua. fodder yeast from dried cells of saccharomyces cerevisiae could be used as a complete mono-component medium for production of highly active spore-delta-endotoxin complexes from b. thur., vars. entomocidus, kurstaki a ... | 1983 | 6666415 |
| production of oxalic acid by some fungi infected tubers. | oxalic acid (as oxalate) was detected in four tubers commonly used for food in nigeria-dioscorea rotundata (white yam), solanum tuberosum (irish potato), ipomoea batatas (sweet potato), and manihot esculenta (cassava). whereas healthy i. batata had the highest oxalic acid content, healthy m. esculenta contained the lowest. when all tubers were artifically inoculated with four fungi-penicillium oxalicum curie and thom, aspergillus niger van tiegh, a. flavus and a. tamarii kita, there was an incre ... | 1983 | 6670294 |
| biological quality and composition of sweet potato protein fragments. | 1981 | 7276386 | |
| changes in composition of the nonprotein-nitrogen fraction of "jewel" sweet potatoes (ipomoea batatas (lam.)) during storage. | 2013 | 7462498 | |
| cloning of the cdna for the human atp synthase oscp subunit (atp5o) by exon trapping and mapping to chromosome 21q22.1-q22.2. | exon trapping was used to clone portions of potential genes from human chromosome 21. one trapped sequence showed striking homology with the bovine and rat atp synthase oscp (oligomycin sensitivity conferring protein) subunit. we subsequently cloned the full-length human atp synthase oscp cdna (gdb/hgmw approved name atp50) from infant brain and muscle libraries and determined its nucleotide and deduced amino acid sequence (embl/genbank accession no. x83218). the encoded polypeptide contains 213 ... | 1995 | 7490082 |
| determination of base specificity of multiple ribonucleases from crude samples. | ribonucleases are widely found on the tissues of living organisms, but the functions of individual ribonucleases are not clear. to facilitate characterization of individual ribonucleases, i have developed a rapid method to separate and identify each ribonuclease from a crude sample by gel electrophoresis instead of by time-consuming purification steps. the ribonucleases in a crude sample are first separated by rna-cast sds-polyacrylamide gel electrophoresis and then eluted from the gel after eth ... | 1994 | 7565655 |
| acaricidal and insecticidal activities of cadina-4,10 (15)-dien-3-one. | the novel assignment of 13c and 1h nmr data for cadina-4,10(15)-dien-3-one obtained from hyptis verticillata is presented. the study revealed that cadina-4,10(15)-dien-3-one possesses chemosterilant activities against the economically important cattle tick, boophilus microplus, and toxic action against adult cylas formicarius the most destructive pest of sweet potato (i pomoea sp.). | 1995 | 7576456 |
| subunit interaction enhances enzyme activity and stability of sweet potato cytosolic cu/zn-superoxide dismutase purified by a his-tagged recombinant protein method. | the coding region of copper/zinc-superoxide dismutase (cu/zn-sod) cdna from sweet potato, ipomoea batatas (l.) lam. cv. tainong 57, was introduced into an expression vector, pet-20b(+). the cu/zn-sod purified by his-tagged technique showed two active forms (dimer and monomer). the amount of proteins of dimer and monomer appeared to be equal, but the activity of dimeric form was seven times higher than that of monomeric form. the enzyme was dissociated into monomer by imidazole buffer above 1.0 m ... | 1995 | 7599315 |
| fractionation and characterization of two forms of peroxidase from oryza sativa. | peroxidase (e.c. 1.11.1.7., hydrogen donor oxidoreductase) is widely distributed and has been isolated from many higher plants (1). the wide distribution of the enzyme suggests that it could be of great biological importance. however the role that it plays in metabolism is not clear due to the large number of reactions it catalyzes and the considerable number of isozymic species (2). in tomato plants, evans and aldridge (3) separated out six isoperoxidases and in a later paper evans reported 12 ... | 1995 | 7603969 |
| nucleotide sequence analysis of two nuclear inclusion body and coat protein genes of a sweet potato feathery mottle virus severe strain (spfmv-s) genomic rna. | recombinant dna molecules containing cdna of a sweet potato feathery mottle virus severe strain (spfmv-s) rna genome were constructed and the partial nucleotide sequences were determined for three dna inserts, which cover 4-2 kb from the 3'-terminus excluding the poly (a) tail. this region of the genome consists of an open reading frame of 1340 amino acids (a.a.) and a 3'-non-translated region of 224 nucleotides. the protein products expected were 6k2 (53 a.a.), nia (435 a.a.), nib (521 a.a.) an ... | 1995 | 7661699 |
| [epidemiology and control of hookworm infection in fujian province]. | fujian province has been known to be endemic for mixed hookworm infection with ancylostoma duodenale as the predominant species. the infection rate was 50% before 1980. as a result of mass treatment starting from 1980, the infection rate was decreased in most areas. and, infection foci were changed from sweet potato fields to the banana, sweatcane and asparagus fields where the infection rate tended to increase. it is stressed that the current endemic characteristics of hookworm infection should ... | 1994 | 7720205 |
| a large-scale, hospital-based case-control study of risk factors of breast cancer according to menopausal status. | we conducted a large-scale, hospital-based case-control study to evaluate differences and similarities in the risk factors of female breast cancer according to menopausal status. this study is based on a questionnaire survey on life style routinely obtained from outpatients who first visited the aichi cancer center hospital between january 1, 1988 and december 31, 1992. among 36,944 outpatients, 1,186 women with breast cancer detected by histological examination were taken as the case group (607 ... | 1995 | 7730137 |
| nucleotide sequence at the 3'-terminal region of sweet potato feathery mottle virus (ordinary strain, spfmv-o) rna. | cdna encoding the 3'-terminal region of sweet potato feathery mottle virus (ordinary strain, spfmv-o) rna was cloned and sequenced. the 2324-bp cdna contained an open reading frame (orf) of 2066 bp followed by an 3' non-coding region and a poly(a) region. the orf covered the coat protein and the carboxy terminus of the nuclear inclusion 'b' protein (nib). | 1994 | 7764983 |
| purification and properties of l-galactono-gamma-lactone dehydrogenase, a key enzyme for ascorbic acid biosynthesis, from sweet potato roots. | l-galactono-gamma-lactone dehydrogenase (l-galactono-gamma-lactone:ferricytochrome c oxidoreductase [ec 1.3.2.3], gldhase) which catalyzes the terminal step in the biosynthesis of l-ascorbic acid (asa) has been purified from roots of sweet potato (ipomoea batatas l., cv. kintoki). highly purified preparation of the gldhase was obtained by three column chromatography steps with a recovery of ca. 1%, after solubilization from mitochondria in sweet potato roots. sds-page exhibited a single band at ... | 1995 | 7775377 |
| crystallization, molecular replacement solution, and refinement of tetrameric beta-amylase from sweet potato. | sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. its subunit consists of 498 amino acid residues (mr 55,880). it has been crystallized at room temperature using polyethylene glycol 1500 as precipitant. the crystals, growing to dimensions of 0.4 mm x 0.4 mm x 1.0 mm within 2 weeks, belong to the tetragonal space group p4(2)2(1)2 with unit cell dimensions of a = b = 129.63 a and c = 68.42 a. the asymmetric unit contains 1 subunit ... | 1995 | 7777485 |
| the relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. interplay between quinol-oxidizing and quinone-reducing pathways. | the dependence of electron flux through quinone-reducing and quinol-oxidizing pathways on the redox state of the ubiquinone (q) pool was investigated in plant mitochondria isolated from potato (solanum tuberosum cv. bintje, fresh tissue and callus), sweet potato (ipomoea batatas) and arum italicum. we have determined the redox state of the q pool with two different methods, the q-electrode and q-extraction techniques. although results from the two techniques agree well, in all tissues tested (wi ... | 1994 | 7813462 |
| a tobacco protein kinase, npk2, has a domain homologous to a domain found in activators of mitogen-activated protein kinases (mapkks). | a cdna (cnpk2) that encodes a protein of 518 amino acids was isolated from a library prepared from poly(a)+ rnas of tobacco cells in suspension culture. the n-terminal half of the predicted npk2 protein is similar in amino acid sequence to the catalytic domains of kinases that activate mitogen-activated protein kinases (designated here mapkks) from various animals and to those of yeast homologs of mapkks. the n-terminal domain of npk2 was produced as a fusion protein in escherichia coli, and the ... | 1995 | 7891653 |
| sweet potato beta-amylase. primary structure and identification of the active-site glutamyl residue. | the complete amino acid sequence of a subunit of sweet potato beta-amylase, a homotetramer, was established by sequence analysis of peptides obtained by digestions with achromobacter protease i and staphylococcus aureus v8 protease and by cyanogen bromide cleavage of the s-carboxymethylated subunit. the subunit of the enzyme is a single polypeptide consisting of 498 amino acid residues. it showed 50-60% identity in the amino acid sequence with those of beta-amylases from soybean and barley, whil ... | 1993 | 8103452 |
| identification of a sweet potato feathery mottle virus isolate from china (spfmv-ch) by the polymerase chain reaction with degenerate primers. | four degenerate oligonucleotide primers derived from conserved regions of the genome of potyviruses have been designed. a combined assay of reverse transcription and the polymerase chain reaction utilizing these primers on total rna extracted from ipomoea purpurea infected with a sweet potato feathery mottle virus isolate from china (spfmv-ch), amplified a 1.35 kb and a 830 bp fragment. these amplified fragments were cloned into the bluescript plasmid and sequenced. the comparison of the sequenc ... | 1993 | 8113341 |
| the presequence of a precursor to the delta-subunit of sweet potato mitochondrial f1atpase is not sufficient for the transport of beta-glucuronidase (gus) into mitochondria of tobacco, rice and yeast cells. | a precursor to the delta-subunit of sweet potato mitochondrial f1atpase (pre-f1 delta) has an amino-terminal (n-terminal) presequence of 45 amino acid residues and its n-terminal 18 residues may form an amphiphilic alpha-helix, which is typical of mitochondrial targeting signals [kimura et al. (1990) j. biol. chem. 265: 6079]. fusion genes consisting of sequences that encoded the 25 (dg25), 46 (dg46) and 73 (dg73) n-terminal amino acids from pre-f1 delta fused to the n-terminus of the coding seq ... | 1993 | 8199776 |
| sweet potato feathery mottle potyvirus (c1 isolate) virion and rna purification. | a procedure for the purification of a peruvian isolate (c1) of sweet potato feathery mottle potyvirus (spfmv) and infective rna has been developed. the use of hepes [n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonic acid] buffer containing urea and sodium edta as a base for tissue extraction and virus suspension enabled good yields of virus (35-50 mg/100 g) to be obtained from nicotiana benthamiana l. domin. a short rna isolation procedure yielded infectious rna, from which ds cdna of nearly geno ... | 1993 | 8227274 |
| cdnas encoding for storage proteins in the tubers of taro (colocasia esculenta schott). | two major protein groups of taro (colocasia esculenta) tuber were purified, and their antisera were used for the screening of the cdna library constructed from poly(a)+ rna of taro tuber. a cdna clone obtained by screening with an anti-12 kd protein antiserum had an insert 1058 bp-long, and an open reading frame for a peptide of 268 amino acids. the analyses of the n-terminal amino acid sequence and in vitro translation product suggested that the protein was synthesized as a peptide with a molec ... | 1993 | 8251188 |
| cloning and characterization of a cdna encoding the cytosolic copper/zinc-superoxide dismutase from sweet potato tuberous root. | a full-length cdna clone encoding a putative copper/zinc-superoxide dismutase (sod) of sweet potato, ipomoea batatas (l.) lam. cv tainong 57, was isolated from a cdna library constructed in lambda gt10 from tuber root mrna. nucleotide sequence analysis of this cdna clone revealed that it comprises a complete open reading frame coding for 152 amino acid residues. the deduced amino acid sequence showed higher homology (78-86%) with the sequence of the cytosolic sod than that of the chloroplast sod ... | 1993 | 8251645 |
| colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants. | various targeting motifs have been identified for plant proteins delivered to the vacuole. for barley (hordeum vulgare) lectin, a typical gramineae lectin and defense-related protein, the vacuolar information is contained in a carboxyl-terminal propeptide. in contrast, the vacuolar targeting information of sporamin, a storage protein from the tuberous roots of the sweet potato (ipomoea batatas), is encoded in an amino-terminal propeptide. both proteins were expressed simultaneously in transgenic ... | 1993 | 8278507 |
| molecular cloning and characterization of cdnas for the gamma- and epsilon-subunits of mitochondrial f1f0 atp synthase from the sweet potato. | mitochondrial f1f0 atp synthases purified from dicotyledonous plants contain six different subunits named alpha, beta, gamma, delta, delta', and epsilon. our previous n-terminal amino acid sequence analyses indicated that the gamma- and epsilon-subunits of the sweet potato mitochondrial f1 correspond to the gamma- and epsilon-subunits of animal mitochondrial f1, respectively (kimura, t., nakamura, k., kajiura, h., hattori, h., nelson, n., and asahi, t. (1989) j. biol. chem. 264, 3183-3186). a cd ... | 1993 | 8349605 |