Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| purification and properties of a plant endonuclease specific for apurinic sites. | an endonuclease which hydrolyzes depurinated dna has been isolated from phaseolus multiflorus enbryos; it has a molecular weight around 40,000. the enzyme is specific for apurinic sites; it has no action on normal dna strands or on alkylated sites, and is without exonulcease activity. the rate of phosphoester bond hydrolysis near apurinic sites is far greater in native than in denatured dna. the endonuclease is not inactivated by 10 mm edta, but is activity is however stimulated by mg2+ or mn2+. ... | 1977 | 16891 |
| ammonia assimilation by rhizobium cultures and bacteroids. | the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ... | 1975 | 234505 |
| cap formation on lymphocytes from patients with leukemic diseases induced by four different lectins. | when ficoll purified peripheral blood lymphocytes were treated with fluorescein conjugated lectins from lentils (lch), castor beans (rca) and phaseolus coccineus beans (l-and e-pha) for 15 min and the percentages of the cap forming cells were examined, the values of leukemic lymphocytes were reduced compared to the values obtained with normal lymphocytes. the reduction was more than half in patients with acute and chronic myelogenous leukemia and immunoblastoma, it was only one quarter in patien ... | 1978 | 274161 |
| [acute poisoning with green beans (phaseolus vulgaris and phaseolus coccineus) (author's transl)]. | 1979 | 471530 | |
| on the receptors of human red cells reacting with phaseolus coccineus l. lectin. | in order to elucidate receptors of proteolytic enzyme-treated red cells which react with phaseolus coccineus l. lectin, the receptors prepared by affinity chromatography were serologically investigated. p. coccineus lectin had high agglutinin activity for bromelin-, papain- and pronase-treated red cells but that for the cells treated with ficin and trypsin was relatively low. analyses of chemical composition revealed that sialic acid of the receptors from normal red cells was considerably much a ... | 1979 | 494265 |
| the lectin binding sites on the membranes of the nuclear envelope, mitochondria and the cell surface of human lymphoblastoid cells. | the membranes of the cell surface, the endoplasmic reticulum, outer and inner mitochondrial leaflet and nuclear envelope were isolated from three human lymphoblastoid cell lines. membrane components were separated by dodecyl sulfate polyacrylamide gel electrophoresis and the gels incubated with the radioiodinated lectins from lentil, castor bean, scarlet runner bean, gorse seed and roman snail. after gel slicing and counting, the molecular weights of the lectin binding sites were determined. abo ... | 1979 | 527941 |
| isolation and chemical characterization of a highly purified phytomitogen from phaseolus coccineus seeds. | an effective lymphocyte mitogen with a low erythroagglutinating ability was isolated from the seeds of phaseolus coccineus l. by ammonium sulphate precipitation and repeated ion-exchange chromatography on modified sephadex. the mitogen stimulated lymphocytes at concentrations as low as 0.25 microgram/ml, the optimum concentration being in the range of 2.5-5.0 microgram/ml. this stimulating effect was completely inhibited by 5 mg/ml methyl alpha-d-mannopyranoside. column gel filtration, disc elec ... | 1978 | 627207 |
| cytological localization of the genes for the four classes of ribosomal rna (25s, 18s, 5.8s and 5s) in polytene chromosomes of phaseolus coccineus. | homologous tritiated 25s, 18s and 5.8s rrnas were used separately for in situ hybridization to the polytene chromosomes of the embryo suspensor cells of phaseolus coccineus. hybridization occurred at the same chromosomal sites which were labeled in previous in situ hybridization experiments with 25 + 18s rrnas in the same material (avanzi et al., 1972), namely: nucleolus organizing system (satellite, nucleolar constriction and organizer) of chromosome pairs i (s1) and v (s2), proximal heterochro ... | 1977 | 858263 |
| [comparative studies on the reactive sites against trypsin of some inhibitors from phaseolus coccineus and phaseolus vulgaris (author's transl)]. | three of the trypsin chymotrypsin inhibitors from the seeds of runner beans (phaseolus coccineus l.), pci 3,4(2), and 5, and three of the inhibitors from the seeds of french beans (phaseolus vulgaris var. nanus), pvi 3, 4, and 5, contain a lysine residue in the reactive site against trypsin. one of the inhibitors from phaseolus coccineus, pci 2, contains an arginine residue there. all seven phaseolus inhibitors investigated are double headed. | 1976 | 1007623 |
| [trypsin and chymotrypsin inhibitors in leguminosae vii. partial amino acid sequences of the trypsin chymotrypsin inhibitor pci 3 from phaseolus coccineus (author's transl)]. | the sequences of amino acid residues at the amino and carboxyl terminus and around the reactive sites of the trypsin chymotrypsin inhibitor pci 3 from the seeds of runner beans (phaseolus coccineus l.) were estimated by aminopeptidase o and carbosypeptidase a degradation before and after enzymatical modification with trypsin or chymotrypsin. beginning at the amino terminus the sequences are :ser-glu-ala-gly-gln-...,...-ile-tyr-lys-ser-gln-(pro)-...with lys-ser as reactive site against trypsin, . ... | 1976 | 1007624 |
| [extrasynthesis of dna and its inhibition by means of a rifampicin derivative in suspension cells of the phaseolus coccineus embryo [proceedings]. | 1976 | 1025656 | |
| immunosuppressive activity of phaseolus coccineus and phaseolus vulgaris extracts in mice. | lectin mixtures from 10 samples of phaseolus coccineus and phaseolus vulgaris beans were used as immunosuppressive agents in the sheep red blood cell (srbc) antibody response of the mouse. some bean samples contain lectins capable of inducing several times better suppression of humoral immunity than concanavalin a and phytohemagglutinin tested before in the same immune system. high dilutions of the 10 bean extracts were shown to agglutinate mouse, rabbit and sheep erythrocytes in vitro but the t ... | 1976 | 1086791 |
| relation between ribosomal rna genes and the dna satellites of phaseolus coccineus. | 1975 | 1165210 | |
| phytohemagglutinin gene expression during seed development of the runner bean, phaseolus coccineus. | the expression of phytohemagglutinin (pha) genes was studied in various tissues during the development of the seeds of phaseolus coccineus cv. hammond's dwarf scarlet by means of northern hybridization and reverse transcription polymerase chain reaction (rt-pcr). the expression is highly development-dependent, starting in the late stage (cotyledons begin to fill the endosperm cavity, i.e. 17 to 24 days after pollination), and tissue-specific. the highest levels of pha-mrna are found in the cotyl ... | 1992 | 1281434 |
| [comparative study of the chemical composition and nutritive value of runner bean (phaseolus coccineus) and of common bean (phaseolus vulgaris)]. | scarlet beans (phaseolus coccineus) is an important food grain legume in the diet of rural populations living in the highlands of méxico, guatemala and other countries of latin america. the present study was conducted to obtain more chemical and nutritive data on this grain legume, because of its importance in agricultural production systems and due to the role it plays in rural diets. ten samples were purchased in the highland markets of guatemala, to compare their physical, chemical and nutrit ... | 1992 | 1308649 |
| ribosomal rna genes of phaseolus coccineus. i. | rdna fragments, including the whole intergenic spacer (igs) region of p. coccineus, were cloned into dephosphorylated puc 13 plasmid. four clones of different insert size were analysed. restriction patterns and physical maps of these length variants (pph1, pph2, pph5, pph6) were performed through complete eco ri cleavage and partial digestion with hpa ii, hae iii, sau 3ai, sma i. evidence was obtained that the length heterogeneity of the four genes was mainly due to a differing number of about 1 ... | 1992 | 1600143 |
| immunocytochemical localization of the storage protein phaseolin in the embryo-suspensor of phaseolus coccineus. | antibodies against the bean storage protein, phaseolin, were induced in rabbits. sections of bean embryos of various age were incubated with the antibodies, and exposed to fluorescein isocyanate (fitc) conjugated goat anti-rabbit immunoglobulin. distinct positive signals were found in vacuoles of the suspensor giant cells. immunofluorescent staining of protein bodies in cotyledon cells, histochemical staining reactions of the sections, as well as gel electrophoretic analysis of the proteins, iso ... | 1991 | 1768728 |
| contact urticaria from runner bean (phaseolus multiflorus). | 1991 | 2044384 | |
| cell-wall polysaccharides and glycoproteins of parenchymatous tissues of runner bean (phaseolus coccineus). | 1. polymers were solubilized from the cell walls of parenchyma from mature runner-bean pods with minimum degradation by successive extractions with cyclohexane-trans-1,2-diamine-nnn'n'-tetra-acetate (cdta), na2co3 and koh to leave the alpha-cellulose residue, which contained cross-linked pectic polysaccharides and hyp-rich glycoproteins. these were solubilized with chlorite/acetic acid and cellulase. the polymers were fractionated by anion-exchange chromatography, and fractions were subjected to ... | 1990 | 2167068 |
| the characteristics of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferases from sansevieria trifasciata leaves and phaseolus coccineus stems. | three different molecular forms of pyrophosphate-dependent phosphofructokinase have been isolated: one from sansevieria trifasciata leaves and two from phaseolus coccineus stems. the form isolated from s. trifasciata has the molecular weight of about 115,000. the apparent molecular weights for the two forms from mung bean were approximately 220,000 and 450,000. all three forms have the same ph optima, an absolute requirement for mg2+ ions both in the forward and reverse reaction, but differ in t ... | 1987 | 2825454 |
| acute bovine pulmonary emphysema caused by the fungus fusarium semitectum. | acute bovine pulmonary emphysema (abpe) is known to occur throughout the world. the documented causes include 3-methylindole, 4-ipomeanol and perilla ketone. although 4-ipomeanol is a phytoallexin from fusarium solani, this is the first reported incidence of toxicity involving fusarium semitectum. this report describes the poisoning of cattle consuming fusarium semitectum contaminated pink half-runner bean refuse. | 1988 | 3388752 |
| distribution of 5-methylcytosine-rich regions in the polytene chromosomes of phaseolus coccineus embryo suspensor as shown by the immunoperoxidase technique. | 5-methylcytosine (5-mc) has been visualized in polytene chromosomes of phaseolus coccineus, scarlet bean using specific antibodies to 5-mc and the immunoperoxidase technique. the results obtained indicate that most heterochromatic regions are methylated, even though the frequency of methylation is highly variable and sometimes low. a preferential binding of anti-5-mc to centromeric heterochromatic blocks was observed. comparison between anti-5-mc binding and the results of hybridization with hig ... | 1986 | 3533573 |
| the effects of galactolipid depletion on the structure of a photosynthetic membrane. | the galactolipids monogalactosyldiglyceride and digalactosyldiglyceride together comprise more than 77% of the photosynthetic membrane lipids of higher plant chloroplasts. we have isolated a lipase from the chloroplasts of runner beans (phaseolus vulgaris) which is highly specific for these galactolipids. this galactolipase promotes the hydrolysis of monogalactosyldiglyceride and digalactosyldiglyceride, in the process liberating two free fatty acids into the membrane bilayer, leaving the residu ... | 1986 | 3771638 |
| osmotica, dimethyl sulfoxide, parahydroxymercuribenzoate, and cyanide change the period of the circadian clock in the pulvini of phaseolus coccineus l. | several substances change the period length of the circadian oscillator in the laminar pulvinus of phaseolus coccineus l. if offered continuously via the transpiration stream to the isolated leaves in continuous light and constant temperature. the osmotica mannitol and peg 6000 (polyethylene glycol) lengthen the circadian period from 27.9 hr (control) to about 29.5 hr. the dose-response of both substances reveal saturation curves. dmso (dimethyl sulfoxide) lengthens the circadian period much mor ... | 1985 | 3939700 |
| hemicellulosic complexes from the cell walls of runner bean (phaseolus coccineus). | the 1 m-koh extract from the depectinated cell walls of parenchymatous tissues of mature runner bean (phaseolus coccineus) on neutralization, dialysis and concentration gave insoluble (hemicellulose a) and soluble (hemicellulose b) carbohydrate complexes in the weight ratio 2:1. both fractions contained polysaccharide, protein and polyphenolic material. the structural features of the carbohydrates were examined by methylation analysis. hemicellulose a contained mainly pectic arabinogalactan, wit ... | 1985 | 4004776 |
| assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism. | the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ... | 1985 | 4016573 |
| influence of valinomycin on circadian leaf movements of phaseolus. | phaseolus coccineus was exposed to valinomycin via the transpiration stream for 5-hr periods. the treatment started at various phases of the free-running circadian rhythm in continuous light; it resulted in phase shifts that varied in a manner dependent on the affected phases. the response curves are similar to those for transient withdrawal of water and for light pulses. the results support the hypothesis that membrane processes are important pacemakers in circadian rhythms. | 1972 | 4506792 |
| pattern of binding of tritiated actinomycin d to phaseolus coccineus polytene chromosomes. i. nucleolus organizing chromosomes. | 1972 | 4635875 | |
| studies on phytohemagglutinins. xx. isolation and characterization of hemagglutinins from scarlet runner seeds (phaseolus coccineus l.). | 1974 | 4854471 | |
| macromolecular synthesis during plant embryogeny: rates of rna synthesis in phaseolus coccineus embryos and suspensors. | 1972 | 5074210 | |
| the enzymic deacylation of phospholipids and galactolipids in plants. purification and properties of a lipolytic acyl-hydrolase from potato tubers. | an enzyme preparation that catalyses the deacylation of mono- and di-acyl phospholipids, galactosyl diglycerides, mono- and di-glycerides has been partially purified from potato tubers. the preparation also hydrolyses methyl and p-nitrophenyl esters and acts preferentially on esters of long-chain fatty acids. triglycerides, wax esters and sterol esters are not hydrolysed. the same enzyme preparation catalyses acyl transfer reactions in the presence of alcohols and also catalyses the synthesis of ... | 1971 | 5154523 |
| plant hormones. ix. phaseic acid, a relative of abscisic acid from seed of phaseolus multiflorus. possible structures. | 1969 | 5373535 | |
| plant hormones. x. the constitution of phaseic acid; a relative of abscisic acid from phaseolus multiflorus. an interpretation of the mass spectrum of phaseic acid and a probable structure. | 1969 | 5373536 | |
| the relative mitogenicities of some french bean (phaseolus vulgaris l.) and runner bean (phaseolus coccineus l.) seed extracts in leukocyte culture. | 1971 | 5560539 | |
| [on a bound gibberellin from phaseolus coccineus l]. | 1964 | 5852163 | |
| autoradiographic evidence of tritiated indolyl-3-acetic acid in epicotyl tissue of phaseolus coccineus. | 1967 | 6065783 | |
| in situ evaluation of the functional state of chromatin by means of quinacrine mustard staining and time-resolved fluorescence microscopy. | a pulsed laser microfluorometer with high spatial and temporal resolution was employed to study the functional state of chromatin, using quinacrine mustard as the fluorescent probe. corresponding segments of polytene chromosomes of embryo suspensor cells of phaseolus coccineus and parenchymal cells of helianthus tuberosus, in phases not involving dna synthesis, were selected as models. the fluorescence decay time turned out to be a discriminating parameter for the chromatin fractions of differin ... | 1984 | 6199331 |
| flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines. | the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ... | 1984 | 6609053 |
| a survey of the nutritional and haemagglutination properties of legume seeds generally available in the uk. | eighty-five samples from fifteen different legume seed lines generally available in the uk were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. from these results the seeds were classified into four broad groups. group a seeds from most varieties of kidney (phaseolus vulgaris), runner (phaseolus coccineus) and tepary (phaseolus acutifolius) beans showed high reactivity with all cell type ... | 1983 | 6615758 |
| purification and partial characterization of an agglutinin from phaseolus coccineus var. 'alubia'. | an agglutination from seeds of 'alubia', a mexican strain of phaseolus coccineus, has been purified by affinity chromatography using physically entrapped stroma. the protein appears to be homogeneous by electrophoresis, molecular sieve chromatography and ultracentrifugation. a molecular species of approx. mr 112,000, with s values of 6.25, 4.52, 4.63 and 4.65 at ph 2.5, 4.5, 7.0 and 9.5, respectively, consisting of four similar subunits (28 kda), and containing 20% w/w glucosamine, is found to b ... | 1982 | 7126589 |
| covalent labelling of the nadph: protochlorophyllide oxidoreductase from etioplast membranes with [3h]n-phenylmaleimide. | [3h]n-phenylmaleimide has been used to covalently label in a specific manner the substrate-protected thiol groups of the enzyme protochlorophyllide reductase. in membrane preparations from oat (avena sativa) and runner-bean (phaseolus vulgaris) seedlings, two related peptides of mol.wts. 35000/37000 and 34000/35000 respectively and showing properties expected of the reductase have been identified, whereas the same technique with barley (hordeum vulgare) extracts resulted in labelling a single pe ... | 1981 | 7306063 |
| glycoproteins from the cell wall of phaseolus coccineus. | 1. the use of a modified sodium chlorite/acetic acid delignification procedure for the solubilization of a hydroxyproline-rich glycoprotein fraction from the depectinated cell walls of phaseolus coccineus is described. 2. the crude glycoprotein was associated with some pectic material; hydroxyproline and serine were the most abundant amino acids, and arabinose, galactose and galacturonic acid the predominant monosaccharides. 3. the bulk of the hydroxyproline is o-glycosidically substituted with ... | 1980 | 7406871 |
| saponins conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4h-pyran-4-one from phaseolus coccineus. | from the hypocotyl of scarlet runner bean (phaseolus coccineus), a new 2,3-dihydro-2,5,-dihydroxy-6-methyl-4h-pyran-4-one conjugated saponin, named soyasaponin alpha a, was isolated together with two known saponins, soyasaponin alpha g and beta g. their structures were elucidated by 1h nmr and 13c nmr spectroscopy and chemical techniques. | 1994 | 7765203 |
| effect of lectins on mouse peritoneal macrophage phagocytic activity. | we studied the in vitro ability of lectin-treated murine peritoneal macrophages to attach and phagocytize particulate antigens. glucose and mannose specific lectins such as con-a and lentil lectin, as well as complex lactosamine residues specific lectins, such as phaseolus vulgaris var. cacahuate and phaseolus coccineus var. alubia, increased the macrophage phagocytic activity towards heterologous erythrocytes, whereas peanut agglutinin, a galactose-specific lectin, diminished the macrophage pha ... | 1994 | 7851961 |
| protoplasts from phaseolus coccineus l. pulvinar motor cells show circadian volume oscillations. | the circadian movement of the lamina of the primary leaf of phaseolus coccineus is mediated by circadian volume changes of the extensor and flexor cells in the upper and lower half of the laminar pulvinus. isolated protoplasts from the extensor, flexor, and flank cells of the pulvinus showed a circadian volume rhythm with a period longer than 24 h. in the case of the flexor protoplasts, we found a period length of 28 h, which is similar to that of the pulvinar cells in situ. in the extensor prot ... | 1994 | 8082224 |
| glycosylation pattern and enzyme activities in atrophic, angulated skeletal muscle fibres from ageing rats. | in this study enzyme activities and lectin binding patterns in skeletal muscle from very old rats were investigated in order to evaluate changes in enzyme activity or carbohydrate expression in senile muscle. activities for adenosine triphosphatase (atpase), succinic dehydrogenase, non-specific esterase and the binding pattern for 31 lectins were investigated in the soleus muscles from very old (36 months) and young (3 months) rats. in ageing muscles atrophic, angulated muscle fibres are frequen ... | 1994 | 8186892 |
| the cytoplasmic male-sterility (cms) determinant of common bean is widespread in phaseolus coccineus l. and phaseolus vulgaris l. | to identify regions of the mitochondrial genome that potentially could specify cytoplasmic male sterility (cms) in phaseolus coccineus (including p. polyanthus), and to define differences amongst p. coccineus lines, mitochondrial (mt)dna restriction patterns and southern blots of total dna from sterile and fertile lines were analysed. by restriction endonuclease mapping we isolated a region which was specific to cms lines flanking an f1-atpase alpha-subunit (atpa) gene. dna sequence analysis of ... | 2017 | 8358821 |
| conformation and activity of phaseolus coccineus var. rubronanus lectin. | the conformation of native and denatured phaseolus coccineus var. rubronanus lectin was studied by circular dichroism (cd) and correlated to the hemagglutinating activity. the far-uv cd spectrum at 25 degrees c showed a broad, negative band around 223 nm and a positive one at 196 nm. cd data analysis of the lectin indicated a beta-sheet-rich protein. at high temperatures, the spectrum was blue-shifted with increasing magnitude; these changes correlated well with the loss of the activity. the con ... | 1993 | 8489702 |
| purification and partial characterization of a new anti-a1 lectin of phaseolus coccineus collected in oaxaca, mexico. | it is well known that phaseolus species contains lectins of various specificities 1-4. runner bean5 and different varieties of ph.coccineus described by ochoa et al6, are not specific to human blood groups. they react indistinctly with erythrocytes of the abo system. however the lectin of ph. lunatus (lima bean)7 is specific to the blood group type a, a classic example of that specificity. sometime ago, we discovered a new lectin in a variety of edible bean of ph. coccineus in the flora of the s ... | 1996 | 8744420 |
| protective mechanism of the mexican bean weevil against high levels of alpha-amylase inhibitor in the common bean. | alpha-amylase inhibitor (alpha ai) protects seeds of the common bean (phaseolus vulgaris) against predation by certain species of bruchids such as the cowpea weevil (callosobruchus maculatus) and the azuki bean weevil (callosobruchus chinensis), but not against predation by the bean weevil (acanthoscelides obtectus) or the mexican bean weevil (zabrotes subfasciatus), insects that are common in the americas. we characterized the interaction of alpha ai-1 present in seeds of the common bean, of a ... | 1996 | 8787024 |
| defining cooking salt intakes for patient counselling and policy making. | the role of salt (nacl) in the development of high blood pressure has been a matter of debate, however, the intersalt study showed that sodium (na) intake in various areas of the world is related to the slope of blood pressure with age. accurate amounts of the total salt intake or that coming from a particular source are needed, both, for physicians who need to consider the salt intake of their patients and for public health workers who are in charge of the implementation of public health progra ... | 1995 | 9230039 |
| hplc profile analysis of oleanene-glucuronides in several edible beans. | hplc analysis and yield of oleanene-glucuronide (og) was done on some commercially available edible beans: seeds of glycine max, glycine max cv. kuromame, phaseolus vulgaris cv. torosuku, phaseolus vulgaris cv. toramame, phaseolus vulgaris cv. taishokintoki, phaseolus coccineus cv. ooshirobana, phaseolus coccineus cv. murasakihanamame, vigna unguiculata cv. chuguro, vigna angularis cv. dainagon, arachis hypogaea, pisum sativum, and vicia faba. all the beans listed above have og, though in varyin ... | 1998 | 9571771 |
| ribosomal rna genes of phaseolus coccineus. iv. structure and comparative-analysis of the intergenic spacer: possible involvement of some nucleotides in the transcription control of coding sequences. sequence of the intergenic spacer of ribosomal genes. | the sequence of the intergenic spacer (igs) of phaseolus coccineus is determined. the igs contains three distinct regions: region a, constant in length; region b, heterogeneous in length among genes, including two very similar segments 162 and 177 bp long, repeated two and nine times respectively in the investigated clone; region c, constant in length, comprising five islands. the putative promoters and the sites of termination, processing and methylation are detected by a comparison with other ... | 1998 | 9773275 |
| molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation. | a major catabolic pathway for the gibberellins (gas) is initiated by 2beta-hydroxylation, a reaction catalyzed by 2-oxoglutarate-dependent dioxygenases. to isolate a ga 2beta-hydroxylase cdna clone we used functional screening of a cdna library from developing cotyledons of runner bean (phaseolus coccineus l.) with a highly sensitive tritium-release assay for enzyme activity. the encoded protein, obtained by heterologous expression in escherichia coli, converted ga9 to ga51 (2beta-hydroxyga9) an ... | 1999 | 10200325 |
| a family of dispersed repeats in the genome of vicia faba: structure, chromosomal organization, redundancy modulation, and evolution. | a family of repeated dna sequences of about 1200 bp in length and bordered by well-conserved, 18 bp inverted repeats (vfb family) was found in the nuclear genome of vicia faba. the structure, chromosomal organization, redundancy modulation and evolution of these sequences were investigated. they are enriched in a+t base pairs (about 40% g+c) and lack any obvious internally repeated motif. a 64%-73% nucleotide sequence identity was found when pairwise comparisons between vfb sequences were carrie ... | 1999 | 10525968 |
| on the reversible conjugation of | after administering [17-d(2)]ga(20) to phaseolus coccineus l. cv. preisgewinner seedlings, [17-d(2)]ga(20)-o-glucoside was identified by liquid chromatography (lc)/esi-tandem mass spectrometry (ms). likewise, by lc/esi-tandem ms the metabolic formation of [17-d(2)]ga(20) glucosyl ester was established. the application of both [17-d(2)]-labeled ga(20) 13-o-glucoside and ga(20) glucosyl ester to phaseolus coccineus l. seedlings resulted in free [17-d(2)]ga(20) by gas chromatography/ms. the results ... | 1999 | 10552132 |
| regional localization of suspensor mrnas during early embryo development. | we investigated gene activity within the giant embryos of the scarlet runner bean (phaseolus coccineus) to gain understanding of the processes by which the apical and basal cells become specified to follow different developmental pathways after division of the zygote. we identified two mrnas, designated g564 and c541, that accumulate specifically within the suspensor of globular-stage embryos. g564 mrna accumulates uniformly throughout the suspensor, whereas c541 mrna accumulates to a higher lev ... | 2001 | 11701878 |
| fluorescent in situ hybridization in plant polytene chromosomes. | polytene chromosomes are found in specialized tissues, with high metabolic activity, of a few angiosperm genera. they differ from diptera polytenics in several aspects, mainly because their chromatids on each chromosome are not tightly paired, nor are they so highly endoreplicated as those of diptera. in situ hybridization with isotopic and non-isotopic probes has been successfully used in plant polytene chromosomes, mainly in phaseolus coccineus and vigna unguiculata, where they have been best ... | 2001 | 11741150 |
| photosynthetic light response curves determined with the leaf oxygen electrode: minimisation of errors and significance of the convexity term. | from photosynthetic studies on a range of monocotyledonous (c-3 and c-4) and dicotyledonous (c-3) plants using a leaf oxygen electrode, we conclude the following. (i) a non-linear model [j.h.m. thornley (1976) mathematical models in plant physiology, academic press, london; b. marshall and p.v. biscoe (1980) j exp bot 31:29-39] significantly better describes the photosynthetic light response curve [rate of photosynthesis (p) versus incident photosynthetic photon flux density (i)] than the freque ... | 2001 | 11762163 |
| the contribution of internode and mesocotyl tissues to root-to-shoot signalling of abscisic acid. | the xylem of first internode of runner bean and of previously etiolated maize mesocotyl segments was perfused with media containing abscisic acid (aba) or abscisic acid glucose ester (aba-ge) in concentrations as they occur under stress conditions. aba-ge passed through the internode and mesocotyl segments unchanged. within 10 min the concentration of aba-ge(xyl) rose to a level similar to that in the external perfusion medium. by contrast, 30-40 min passed before the concentration of free aba i ... | 2002 | 11807133 |
| primary structures of alpha- and beta-subunits of alpha-amylase inhibitors from seeds of three cultivars of phaseolus beans. | the primary structures of three alpha-amylase inhibitors (tai, dai, and mai-2) consisting of glycoprotein subunits alpha and beta from the respective seeds of three cultivars of phaseolus beans, toramame (phaseolus vulgaris l.), daifukumame (phaseolus vulgaris l.), and murasakihanamame (phaseolus coccineus l.) were determined by sequencing the peptide fragments derived from their enzymatic digestions. major sugar chains of the inhibitors were also assessed by analyzing glycopeptides in the enzym ... | 2002 | 11902670 |
| [physical, culinary and nutritional characterization in dry bean from the highlands of mexico]. | in the improvement of a given crop species, knowledge on the grain quality and related traits in the progenitors used in the breeding process is needed to establish their usefulness as a source of a given character, and as well as to plan the proper combinations between progenitors. the aim of the present research was to characterize a group of 49 genotypes of phaseolus vulgaris and one of phaseolus coccineus, in relation to physical, cooking and nutritional grain traits. cultivar blanco tlaxcal ... | 2002 | 12184152 |
| the isolation of carbohydrate-binding peptide from scarlet runner bean lectin. | the carbohydrate-binding peptide fragment of scarlet runner bean (phaseolus coccineus var. rubronanus) lectin has been prepared by trypsin digestion. the carbohydrate-binding peptide was isolated from digested solution by affinity chromatography on thyroglobulin-sepharose column, bio-gel p-4 gel filtration column and reverse phase hplc on c-8 column. the fraction of peak i from hplc which bound specifically with man(8)glcnac(2) was demonstrated by using dot blot technique with [(3)h]- man(8)glcn ... | 1997 | 12219212 |
| sequence analysis of the oligosaccharides from scarlet runner bean lectin. | two oligosaccharide chains from scarlet runner bean (phaseolus coccineus var. rubronanus) lectin (pcl) were obtained by hydrazinolysis, bio-gel p-2 column chromatography, nabh(4) reduction and then separated on a bio-gel p-2 column. the oligosaccharides were methanolysed, acetylysed and then transformed into derivatives of trimethylsilane. gas chromatographic studies of these two derivatives showed that their compositions were: man(8)xylglcnac(2) (oligosaccharide i) and man(6)xylglcnac(2)fuc (ol ... | 1996 | 12232596 |
| evidence for the direct 2beta- and 3beta-hydroxylation of [2h2]ga20-13-o-[6'-2h2]glucoside in seedlings of phaseolus coccineus. | [17-2h2]ga20-13-o-[6'-2h2]glucoside was synthesized and applied to seedlings of phaseolus coccineus l. after incubation for 72 h the conjugate metabolites were purified and shown by lc-esi-tandem-ms and gc-ms to be [17-2h2]ga1-13-o-[6'-2h2]glucoside and [17-2h2]ga29-13-o-[6'-2h2]glucoside. this is the first evidence for the conversion of intact ga-o-glucosides, and represents an additional metabolic pathway of the gibberellin metabolism in p. coccineus l. the results indicate that intact ga-o-gl ... | 2002 | 12354189 |
| molecular research on the genetic diversity of polish varieties and landraces of phaseolus coccineus l. and phaseolus vulgaris l. using the rapd and aflp methods. | the aim of our research was to evaluate the genetic diversity among 25 commercial varieties registered in poland and 14 landraces of phaseolus vulgaris var. nanus asch. (the dwarf common bean) and phaseolus coccineus l. (the runner bean) maintained in the national centre of plant genetic resources in radzików. an additional goal of this study was to compare the precision and efficiency of two techniques of pcr (rapd and aflp), used to estimation the genetic diversity of bean. the breeding variet ... | 2002 | 12378235 |
| contact urticaria from runner bean (phaseolus coccineus). | 2002 | 12492534 | |
| activation tagging of a dominant gibberellin catabolism gene (ga 2-oxidase) from poplar that regulates tree stature. | we identified a dwarf transgenic hybrid poplar (populus tremula x populus alba) after screening of 627 independent activation-tagged transgenic lines in tissue culture, greenhouse, and field environments. the cause of the phenotype was a hyperactivated gene encoding ga 2-oxidase (ga2ox), the major gibberellin (ga) catabolic enzyme in plants. the mutation resulted from insertion of a strong transcriptional enhancer near the transcription start site. overexpression of the poplar ga2ox gene (ptaga2 ... | 2003 | 12857810 |
| examination of the gibberellins of zea mays and phaseolus multiflorus using thin-layer chromatography. | 1964 | 14210963 | |
| lipid components of leaves. v. galactolipids, cerebrosides, and lecithin of runner-bean leaves. | 1964 | 14229671 | |
| hydrolysis of monogalactosyl and digalactosyl diglycerides by specific enzymes in runner-bean leaves. | 1964 | 14231719 | |
| chromatographic and radioisotopic investigations of the lipid components of runner bean leaves. | 1960 | 14404717 | |
| growth responses of three legume species exposed to simulated acid rain. | seedlings of vicia faba l., phaseolus multiflorus l. and pisum sativum l. were raised during exposure to simulated acid rainfall treatments of phs 5.6, 4.5, 3.5 and 2.5 at a rate of 30 mm per week. all three species were found to be adversely affected by the more acid ph 3.5 and ph 2.5 treatments after 7-8 weeks of exposure. there were total plant dry weight reductions of 40% for v. faba, 31% for p. sativum and 28% for p. multiflorus exposed to the ph 2.5 treatment, as compared to those grown in ... | 1989 | 15092352 |
| coccinin, an antifungal peptide with antiproliferative and hiv-1 reverse transcriptase inhibitory activities from large scarlet runner beans. | an antifungal peptide, designated coccinin, with a molecular mass of 7kda and an n-terminal sequence resembling those of defensins, was purified from the seeds of large scarlet runner beans (phaseolus coccineus cv. 'major'). the peptide isolated was unadsorbed on deae-cellulose, and adsorbed on affi-gel blue gel and mono s. the peptide excerted antifungal activity on a number of fungal species including botrytis cinerea, coprinus comatus, fusarium oxysporum, mycosphaerella arachidicola, physalos ... | 2004 | 15572193 |
| phaseococcin, an antifungal protein with antiproliferative and anti-hiv-1 reverse transcriptase activities from small scarlet runner beans. | from the seeds of small scarlet runner beans (phaseolus coccineus 'minor'), an antifungal protein with an n-terminal sequence homologous to those of defensins was isolated. the antifungal protein bound to affi-gel blue gel and mono s but it did not bind to deae-cellulose. it was further purified by gel filtration on a superdex peptide column. it exhibited a molecular mass of 5422 da as determined by mass spectrometry. the protein, designated as phaseococcin, suppressed mycelial growth in a numbe ... | 2005 | 15864329 |
| expression of two genes encoding gibberellin 2- and 3-oxidases in developing seeds of phaseolus coccineus. | we have isolated pcga3ox1, a cdna clone from developing runner bean (phaseolus coccineus) seeds that shows significant amino acid homology with the gibberellin (ga) 3-oxidases. a recombinant fusion protein of pcga3ox1 converted ga20 and ga9 to ga1 and ga4, respectively. in situ hybridization results showed that transcripts of this gene accumulate specifically within the suspensor of globular-stage embryos. pcga3ox1 mrna begins to accumulate in the epidermal cells of the embryo proper and is also ... | 2005 | 15894806 |
| application of dl-beta-aminobutyric acid (baba) as a root drench to legumes inhibits the growth and reproduction of the pea aphid acyrthosiphon pisum (hemiptera: aphididae). | dl-beta-aminobutyric acid (baba) is a non-protein amino acid that is an effective inducer of resistance against a variety of plant pathogens. however, examples of baba-induced resistance against insect herbivores have not been reported. we applied baba as a soil drench to legumes and monitored its effects on the pea aphid acyrthosiphon pisum (harris). on tic bean (vicia faba var. minor), baba increased aphid mortality, caused a reduction in the mean relative growth rate of individual insects and ... | 2005 | 16197565 |
| a provisional database for the silicon content of foods in the united kingdom. | si may play an important role in bone formation and connective tissue metabolism. although biological interest in this element has recently increased, limited literature exists on the si content of foods. to further our knowledge and understanding of the relationship between dietary si and human health, a reliable food composition database, relevant for the uk population, is required. a total of 207 foods and beverages, commonly consumed in the uk, were analysed for si content. composite samples ... | 2005 | 16277785 |
| sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein banding patterns among rhizobium leguminosarum biovar phaseoli strains isolated from the mexican bean phaseolus coccineus. | several rhizobial strains were isolated from phaseolus coccineus root nodules and were determined to be rhizobium leguminosarum biovar phaseoli strains after reinfection of the same host plant. these strains were characterized by cultural procedures (growth on different carbon sources and intrinsic antibiotic resistance) and electrophoretic procedures (sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total proteins). our results showed that these rhizobia are very similar to each oth ... | 1993 | 16349098 |
| genetic structure of rhizobium etli biovar phaseoli associated with wild and cultivated bean plants (phaseolus vulgaris and phaseolus coccineus) in morelos, mexico. | the genetic structure of rhizobium etli biovar phaseoli was determined for five populations in three different locations in the state of morelos, mexico, by using starch gel electrophoresis for five to nine polymorphic loci. two populations were sampled during two different years from nodules of cultivated and wild common bean plants (phaseolus vulgaris). the three other populations were associated with wild runner beans (p. coccineus) and sampled during 1988. the rhizobium populations differ ge ... | 1994 | 16349234 |
| lipid peroxidation in cadmium-treated phaseolus coccineus plants. | lipid peroxidation was examined in phaseolus coccineus plants treated with 25 microm cadmium (cd) for 12 days at two different growth stages of the primary leaves. in the leaves of cd-treated young plants, the content of free fatty acids was higher only after 8 days of plant exposure to the metal, lipoxygenase activity at ph 7.0 was enhanced after 6 and 8 days of cd action, but at ph 8.0 it was higher than control, especially after 8 days. non-enzymatic lipid peroxidation was higher after 6 days ... | 2006 | 16418896 |
| the level of jasmonic acid in arabidopsis thaliana and phaseolus coccineus plants under heavy metal stress. | the effect of heavy metal stress as a potent abiotic elicitor for triggering an accumulation of jasmonic acid (ja) was investigated. copper and cadmium in in vivo conditions induced accumulation of jasmonates in mature leaves of arabidopsis thaliana and in young and oldest phaseolus coccineus plants. the dynamics of jasmonate accumulation showed a biphasic character in both plants. in the first phase, after 7 (a. thaliana) or 14h (p. coccineus) of exposure to cu or cd, a rapid increase of ja lev ... | 2005 | 16425452 |
| light-induced phase shifts of circadian leaf movements of phaseolus: comparison with the effects of potassium and of ethyl alcohol. | leaves of phaseolus coccineus were exposed to high concentrations of kcl (0.1-0.3 m) for periods of 4-5 hr, or to ethyl alcohol (5-30%) for periods of 2 hr. the treatment started at various phases of the free-running circadian rhythm in continuous light. whereas the k(+) pulses induced only advance phase shifts, i.e., shortening of the periods, the alcohol pulses caused stronger advances and also slight delays, i.e., lengthening of the periods. these delays became stronger with lower concentrati ... | 1973 | 16592119 |
| maintained electrical polarities in region of the axillary buds in phaseolus multiflorus. | 1936 | 16653348 | |
| bud regeneration and electrical polarities in phaseolus multiflorus. | 1938 | 16653485 | |
| electrical response of phaseolus multiflorus to electrical currents. | 1939 | 16653562 | |
| effect of hydrolytic enzymes on the photosynthetic efficiency and morphology of chloroplasts. | both lipase from runner beans and a protease (pronase) initially cause increased intensity dependence of the dcpip hill reaction of spinach chloroplasts. this is followed by an increase in the extrapolated zero intensity quantum requirement. pronase treatment of the chloroplasts causes rapid changes in absorption and ord spectra, whereas the effect of lipase treatment is much less pronounced. long treatments of the thylakoids with the lipase and protease cause unique morphological changes within ... | 1966 | 16656445 |
| translocation of indole-3-acetic acid-1'-c and tryptophan-1-c in seedlings of phaseolus coccineus l. and zea mays l. | indole-3-acetic acid-1'-(14)c (iaa-(14)c) and tryptophan-1-(14)c injected in small amounts into cotyledons of phaseolus coccineus l. seedlings were found to be translocated acropetally into the epicotyls and young shoots. similarly iaa-(14)c was translocated acropetally into coleoptiles of zea mays following injection into the endosperms. labeled metabolites of the injected compounds were also extractable from shoot tissue. however, evidence that iaa-(14)c itself was translocated acropetally was ... | 1967 | 16656664 |
| metabolism of h-gibberellin a(1) and h-gibberellin a(4) by phaseolus coccineus seedlings. | [(3)h]-gibberellin a(1) (ga(1)) and (3)h-ga(4) were applied separately to phaseolus coccineus seedlings grown under red light. (3)h-ga(1) was converted to a compound with gas-liquid radiochromatography retention times identical to those of ga(8). (3)h-ga(4) underwent conversion to at least three metabolites, none of which corresponded to ga(1-38). the rate of metabolism of (3)h-ga(4) was significantly higher than that of (3)h-ga(1). | 1975 | 16659025 |
| differences between adenosine triphosphatases from monocotylous and dicotylous plants. | the mitochondrial membrane-bound atpases of several plants were investigated. two distinct types were encountered. the mitochondrial atpases of castor bean (ricinus communis var. zanzibarensis, l.), cauliflower (brassica oleracea, l.), and scarlet runner (phaseolus coccineus, l.) were found to be inhibited by oligomycin, to have elevated molecular weights when separated from the organelles by ultrasonication and ammonium sulfate treatment and, subsequent to purification, to be cold-labile. on th ... | 1977 | 16659806 |
| distinction between nuclear satellite dnas and chloroplast dna in higher plants. | triton x-100 solubilized chloroplast dna but not nuclear dna from a mixture of chloroplasts and nuclei. the buoyant density of chloroplast dna was different from that of the satellite dna in all of the species examined (phaseolus coccineus, cucumis sativus, cucumis melo, antirrhinum majus, vicia faba, oenothera fruiticosa youngii). chloroplast dna constituted between 4.3% and 0.25% of the total leaf dna in these species, and was present as 5 to 20 copies in each chloroplast. | 1978 | 16660649 |
| identification of traumatin, a wound hormone, as 12-oxo-trans-10-dodecenoic acid. | 12-oxo-trans-10-dodecenoic acid (trans-10-oda) is an oxidation product of polyunsaturated fatty acids in plant tissues. the structural similarity of trans-10-oda and traumatic acid, a compound considered to be a wound hormone, suggested that trans-10-oda might be a precursor of traumatic acid. both trans-10-oda and traumatic acid were active in the wehnelt bean assay. the results were more consistent with trans-10-oda than with traumatic acid. cucumber (cucumis sativus l. var. national pickling) ... | 1979 | 16660762 |
| fatty acids and circadian rhythms in phaseolus coccineus: effects of light, temperature, and chemicals. | five major fatty acids, palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic (18:3), were identified in polar lipid extracts from pulvini of samanea saman and phaseolus coccineus. in p. coccineus their distribution varied quantitatively in the laminar pulvinus, petiolar pulvinus, petiole, stem, leaf and root. short pulses of red light did not greatly affect the relative quantities of fatty acids in dark grown p. coccineus, but a 30-minute exposure of red light generally ... | 1979 | 16660990 |
| distribution and properties of a potassium-dependent asparaginase isolated from developing seeds of pisum sativum and other plants. | asparaginase (ec 3.5.1.1) was isolated from the developing seed of pisum sativum. the enzyme is dependent upon the presence of k(+) for activity, although na(+) and rb(+) may substitute to a lesser extent. maximum activity was obtained at k(+) concentrations above 20 millimolar. potassium ions protected the enzyme against heat denaturation. the enzyme has a molecular weight of 68,300.asparaginase activity developed initially in the testa, with maximum activity (3.6 micromoles per hour per seed) ... | 1980 | 16661136 |
| electrical membrane properties and circadian rhythm in extensor cells of the laminar pulvini of phaseolus coccineus l. | circadian changes of three parameters of electrical properties in extensor cells of the laminar pulvini of phaseolus coccineus l. have been recorded by standard microelectrode techniques. in the low turgor state of the cells, the resting potential and the cn(-) (1 millimolar nacn) induced depolarizations are large, while the k(+)-induced voltage changes are small, and vice versa in the high turgor state. these results demonstrate that during a circadian cycle the low turgor state of the extensor ... | 1980 | 16661315 |
| n abundance of nodules as an indicator of n metabolism in n(2)-fixing plants. | this paper expands upon previous reports of (15)n elevation in nodules (compared to other tissues) of n(2)-fixing plants. n(2)-fixing nodules of glycine max (soybeans), vigna unguiculata (cowpea), phaseolus vulgaris (common bean), phaseolus coccineus (scarlet runner bean), prosopis glandulosa (mesquite), and olneya tesota (desert ironwood) were enriched in (15)n. nodules of vicia faba (fava beans), arachis hypogaea (peanut), trifolium pratense (red clover), pisum sativum (pea), lathyrus sativus ... | 1982 | 16662517 |
| gibberellins in suspensors of phaseolus coccineus l. seeds. | analysis of extracts from 6300 (1.2 grams fresh weight) phaseolus coccineus suspensors by combined gas chromatography-mass spectrometry has demonstrated the presence of five c(19)-gibberellins, ga(1), ga(4), ga(5), ga(6), ga(8), and one c(20)-ga, ga(44). the major gas present were ga(1) and ga(8). data are discussed in relation to previous results obtained in p. coccineus seed as well as in the embryo-suspensor system. | 1986 | 16665010 |
| metabolism of c-zeatin in phaseolus embryos : occurrence of o-xylosyldihydrozeatin and its ribonucleoside. | the metabolism of trans-[8-(14)c]zeatin was examined in embryos of phaseolus acutifolius a. gray p.i. 321637 and phaseolus coccineus lam. cvs scarlet runner and desiree. in both species zeatin was converted to ribosylzeatin, ribosylzeatin 5'-monophosphate, o-glucosyl-9-ribosylzeatin and the recently discovered o-xylosyl derivatives of zeatin and ribosylzeatin (turner, je, dws mok, mc mok, g shaw 1987 proc natl acad sci usa. in press). two new metabolites, identified by enzyme degradation and gas ... | 1987 | 16665486 |
| gibberellins in embryo-suspensor of phaseolus coccineus seeds at the heart stage of embryo development. | gibberellins (gas) in suspensors and embryos of phaseolus coccineus seeds at the heart stage of embryo development were analyzed by combined gas chromatography-mass spectrometry (gc-ms). from the suspensor four c(19)-gas, ga(1), ga(4), ga(5), ga(6), and one c(20) ga, ga(44), were identified. from the embryo, five c(19)-gas ga(1), ga(4), ga(5), ga(6), ga(60) and two c(20) gas, ga(19) and ga(44) were identified. the data, in relation to previous results, suggest a dependence of the embryo on the s ... | 1989 | 16667026 |
| pattern of variations in abscisic acid content in suspensors, embryos, and integuments of developing phaseolus coccineus seeds. | free abscisic acid (aba) content in suspensors, embryos, and integuments was determined during seed development of phaseolus coccineus. a highly specific and sensitive solid-phase radioimmunoassay based on a monocional antibody raised against free (s)-aba was used for aba quantification. very small amounts of aba were detected in the suspensor during initial stages of development; later two peaks of aba occurred. levels of aba in the embryo and integument show a coincident triphasic distribution ... | 1990 | 16667915 |