Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| stability of polyadenylic and polyadenylated ribonucleic acids in radish (raphanus sativus) seedlings. | the stability of polyadenylic acid and polyadenylated rna was investigated in young radish (raphanus sativus) seedlings. we first studied the decay of poly(a) content, using a [3h]poly(u) assay, following a complete block of transcription by cordycepin (200 microgram/ml). two lifetime classes of polyadenylic acid have been determined in these seedlings: a short-lived component with a half-life of 30 min which represents 60% of poly(a) and a more stable component with varying half-lives of which ... | 2000 | 317002 |
| analysis of plant genomes. iv. isolation and characterization of satellite dna components from two dicotyledons cucumber (cucumis sativus) and radish (raphanus sativus). | satellite dna fractions from cucumber and radish, two plants having low dna contents and relatively small chromosomes, were isolated and characterized. reassociation studies of satellite and total nuclear dna showed that the satellite fractions in these two plants contain most of the rapidly reassociating dna. cucumber satellite i was found to contain one major component (70% of the total satellite) having a density of 1.706 g/cm3 and a tm of 90.5 degrees c and a minor component with a density o ... | 1978 | 688067 |
| isolation of monomeric cytochrome f from japanese radish and a mechanism of autoreduction. | monomeric cytochrome f from japanese radish (raphanus sativus l. var acanthiformis makino) leaves was isolated in a homogeneous state with an a420.5/a277 of 7.6. radish cytochrome f is a single polypeptide chain with a molecular weight of about 33,000. the midpoint potential is 350 mv. the amino acid analysis indicates the existence of 3 residues of half-cystine. radish cytochrome f contains one thiol group which reacts with 5,5'-dithiobis(2-nitrobenzoic acid) only after denaturation by sodium d ... | 1978 | 701259 |
| [stimulatory action of brassica oleracea var. sabauda (savoy) on mycobacteria and mycobacterial infection (author's transl)]. | eleven vegetable species were investigated for the presence of growth-stimulating activity on mycobacteria. it was found that savoy juice (brassica oleracea var. sabauda), given orally, posessed a pronounced stimulating effect on m. tuberculosis, m. bovis, m. kansasii, m. avium and m. intracellulare serotype davis in the serum of experimental animals when tested in auxanograms (table 1). another 2 species, paprica (capsicum frutescens) and radish (raphanus sativus var. albus) showed only insigni ... | 1978 | 746996 |
| [virus infestation of garden radish (raphanus sativus l. var. sativus) (author's transl)]. | in july 1974 a mosaic disease was stated in seed plants of garden radish near aschersleben which spread more and more within some weeks and eventually infected over 40 per cent of the stand. investigations in comparison with healthy seed plants demonstrated the following average losses induced by the disease: number of pods 51 per cent, seed weight 67 per cent, number of seeds per plant 53 per cent, weight of 1,000 seeds 33 per cent. nearly unaffected were: number of seeds per pod, percentage of ... | 1976 | 1037175 |
| biodegradation of phenoxyacetic acid in soil by pseudomonas putida pp0301(pr0103), a constitutive degrader of 2,4-dichlorophenoxyacetate. | the efficacy of using genetically engineered microbes (gems) to degrade recalcitrant environmental toxicants was demonstrated by the application of pseudomonas putida pp0301(pr0103) to an oregon agricultural soil amended with 500 micrograms/g of a model xenobiotic, phenoxyacetic acid (paa). p. putida pp0301(pr0103) is a constitutive degrader of 2,4-dichlorophenoxyacetate (2,4-d) and is also active on the non-inducing substrate, paa. paa is the parental compound of 2,4-dichlorophenoxyacetic acid ... | 1992 | 1344988 |
| the effect of extracts from garden radish (raphanus sativus) and horseradish (amoracia rusticana) on platelet functional activity in vitro. | 1992 | 1492118 | |
| spatial pattern of cdc2 expression in relation to meristem activity and cell proliferation during plant development. | the p34 protein kinase encoded by the cdc2 gene is a key component of the eukaryotic cell cycle required for the g1- to s-phase transition and entry into mitosis. to study the regulation of plant meristem activity and cell proliferation, we have examined the tissue-specific accumulation of cdc2 transcripts in arabidopsis thaliana and the related crucifer radish (raphanus sativus) by in situ hybridization using a. thaliana cdc2 cdna sequences as a probe. cdc2 transcripts accumulated in leaf primo ... | 1992 | 1502145 |
| detection and prevalence of listeria monocytogenes in the agricultural ecosystem. | the sensitivity of four different enrichment procedures to detect listeria monocytogenes in the presence of high levels of streptococcus faecalis was investigated. defined mixed cultures of strep. faecalis and l. monocytogenes gave better results with one-stage enrichment techniques. for manure samples, however, two-stage enrichment techniques gave the best performance. the so-called cold enrichment techniques were found to be unsatisfactory for samples from natural environments. the following m ... | 1991 | 1955415 |
| a fern spore storage protein is genetically similar to the 1.7 s seed storage protein of brassica napus. | the ostrich fern, matteuccia struthiopteris l., contains two globulin spore storage proteins of 2.2 s and 11.3 s, with physical characteristics similar to those of seed storage proteins of brassica napus (rapeseed) and raphanus sativus (radish). by the use of a cloned cdna that encodes the 1.7 s b. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear dna, and a 900-nucleotide homologous mrna was detected in developing spores. in vitro translation ... | 1988 | 3242496 |
| detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme a reductase from radish seedlings. | 3-hydroxy-3-methylglutaryl-coa reductase (nadph) was solubilized with polyoxyethylene ether (brij) w-1 from a heavy-membrane fraction, sedimented at 16000 x g from a cell-free homogenate of four-day-old, dark-grown radish seedlings (raphanus sativus l.). approximately 350-fold purification of the solubilized enzyme activity was achieved by (nh4)2so4 precipitation followed by column chromatography on deae-sephadex a-50, blue-dextran-agarose and hmg-coa-hexane-agarose. the presence of detergent, w ... | 1986 | 3510865 |
| [immunomodulation with natural products. i. effect of an aqueous extract of raphanus sativus niger on experimental influenza infection in mice]. | a raphanus sativus niger water extract was administered by intranasal instillations to mice before inoculation of the influenza virus a/pr 8/34 (h1n1) strain by the same route. the extract ensured some protection against the experimental influenza infection. a significant decrease of the hemagglutinin titre of the mouse lung homogenate was noted, as well as a decrease of the mortality rate and a significant increase of the rate of survival as compared to the untreated controls. | 1987 | 3617495 |
| microtubules in root hairs. | the microtubules of root hairs of raphanus sativus, lepidium sativum, equisetum hyemale, limnobium stoloniferum, ceratopteris thalictroides, allium sativum and urtica dioica were investigated using immunofluorescence and electron microscopy. arrays of cortical microtubules were observed in all hairs. the microtubules in the hairs show net axial orientations, but in allium and urtica helical microtubule patterns are also present. numerical parameters of microtubules in raphanus, equisetum and lim ... | 2014 | 4066793 |
| a light and electron microscope study of small, spherical nuclear bodies in meristematic cells of allium cepa, vicia faba, and raphanus sativus. | interphase, preprophase, and prophase nuclei of meristematic cells of allium cepa, vicia faba, and raphanus sativus are characterized by the presence of spherical bodies approximately 1 micro in diameter. these structures are feulgen-negative but stain metachromatically with azure b, as the nucleolus, following fixation with glutaraldehyde. at the ultrastructural level, they consist predominantly of fibrils estimated to be between 70 and 100 a in diameter which greatly resemble those found withi ... | 1965 | 5323608 |
| a new species of the genus aphelenchoides fischer, 1894 (nematoda: aphelenchoididae) from rootlets of radish (raphanus sativus) in lucknow. | 1969 | 5381125 | |
| development and loss of toluene diisocyanate reactivity: immunologic, pharmacologic, and provocative challenge studies. | toluene diisocyanate (tdi) sensitivity accompanied by nonspecific bronchial hyperresponsiveness occurs in approximately 5% of occupationally exposed workers. we report the case of a 32-yr-old worker followed longitudinally after removal from isocyanate exposure. tdi reactivity was lost 11 mo after removal from exposure and nonspecific bronchial hyperresponsiveness resolved after 17 mo. bronchial reactivity to radishes (raphanus sativus), which developed concurrently with tdi reactivity, was lost ... | 1982 | 6288789 |
| a view of plant dehydrins using antibodies specific to the carboxy terminal peptide. | dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ... | 1993 | 7693020 |
| production and characterization of antibodies to the beta-(1-->6)-galactotetraosyl group and their interaction with arabinogalactan-proteins. | rabbit antisera were raised against beta-(1-->6)-galactotetraose coupled to bovine serum albumin (gal4-bsa). the antisera reacted with arabinogalactan-proteins (agps) isolated from seeds, roots, or leaves of radish (raphanus sativus l.) as revealed by immunodiffusion analysis. extensive removal of alpha-l-arabinofuranosyl residues from these agps enhanced the formation of precipitin with the antisera. the antisera did not react with such other polysaccharides as soybean arabinan-4-galactan, beta ... | 1993 | 7763824 |
| small cysteine-rich antifungal proteins from radish: their role in host defense. | radish seeds have previously been shown to contain two homologous, 5-kd cysteine-rich proteins designated raphanus sativus-antifungal protein 1 (rs-afp1) and rs-afp2, both of which exhibit potent antifungal activity in vitro. we now demonstrate that these proteins are located in the cell wall and occur predominantly in the outer cell layers lining different seed organs. moreover, rs-afps are preferentially released during seed germination after disruption of the seed coat. the amount of released ... | 1995 | 7780308 |
| expression of functional raphanus sativus antifungal protein in yeast. | rs-afp2 is a 51 amino acid cysteine-rich peptide isolated from radish (raphanus sativus) seeds that exhibits potent inhibitory activity against filamentous fungi. a cdna clone encoding the rs-afp2 preprotein was modified by recombinant dna methods to allow expression in the yeast saccharomyces cerevisiae. this peptide was expressed in yeast as a fusion protein carrying at its n-terminus the prepro-sequences derived from the precursor of the yeast pheromone mating factor alpha 1. these sequences ... | 1994 | 8034047 |
| purification and sequencing of radish seed calmodulin antagonists phosphorylated by calcium-dependent protein kinase. | a family of radish (raphanus sativus) calmodulin antagonists (rcas) was purified from seeds by extraction, centrifugation, batch-wise elution from carboxymethyl-cellulose, and high performance liquid chromatography (hplc) on an sp5pw cation-exchange column. this rca fraction was further resolved into three calmodulin antagonist polypeptides (rca1, rca2, and rca3) by denaturation in the presence of guanidinium hcl and mercaptoethanol and subsequent reverse-phase hplc on a c8 column eluted with an ... | 1993 | 8278508 |
| alpha-l-fucosyltransferases from radish primary roots. | a novel alpha-l-fucosyltransferase capable of transferring l-fucose (l-fuc) from gdp-l-fuc to the o-2 of alpha-l-arabinofuranosyl residue (gdp-l-fuc:alpha-l-arabinofuranoside 2-alpha-l-fucosyltransferase) has been found in the microsomal fraction of primary roots from 6-d-old radish (raphanus sativus l.) seedlings. enzyme activity was measured fluorometrically at 25 degrees c using a pyridylaminated trisaccharide, l-arabinofuranosylf alpha(1-->3)d-galactopyranosyl beta(1-->6)d-galactose (aragalg ... | 1996 | 8742340 |
| cloning of a cdna encoding cytosolic acetoacetyl-coenzyme a thiolase from radish by functional expression in saccharomyces cerevisiae. | a cdna coding for radish (raphanus sativus l.) acetoacetyl-coenzyme a thiolase (aact) was cloned by complementation of the erg10 mutation affecting aact in yeast (saccharomyces cerevisiae). the longest reading frame encodes a protein of 406 amino acids with a predicted relative molecular weight of 42,032, with significant similarities to eukaryotic and prokaryotic thiolases. there is no evidence for the presence of a leader peptide characteristic, e.g. of glyoxysomal thiolase. yeast transformant ... | 1996 | 8756496 |
| effects of semi-purified dietary fibers isolated from lagenaria siceraria, raphanus sativus and lentinus edodes on fecal steroid excretions in rats. | rats were fed diets, each of which contained 5% of the semi-purified dietary fiber (df) preparations from three kinds of foods, continuously for a total of 7 weeks. the fat level was varied, and cholesterol (chol) was added toward the end of the experimental period. after 7 weeks, decreases of serum and liver chol concentrations were observed in the shiitake (lentinus edodes) group when compared to the cellulose (cp) group. in the absence of chol, fecal excretions of several secondary bile acids ... | 1996 | 8780968 |
| mutational analysis of a plant defensin from radish (raphanus sativus l.) reveals two adjacent sites important for antifungal activity. | mutational analysis of rs-afp2, a radish antifungal peptide belonging to a family of peptides referred to as plant defensins, was performed using polymerase chain reaction-based site-directed mutagenesis and yeast as a system for heterologous expression. the strategy followed to select candidate amino acid residues for substitution was based on sequence comparison of rs-afp2 with other plant defensins exhibiting differential antifungal properties. several mutations giving rise to peptide variant ... | 1997 | 8995418 |
| [seasonality of parasites and intestinal bacteria in vegetables that are consumed raw in costa rica]. | in costa rica, a total of 640 samples from eight different vegetables used for raw consumption, were analyzed for the presence of intestinal parasites and fecal coliforms. eighty samples of each vegetable were analyzed, forty during the dry season and forty in the rainy. a greater, but unsignificant (p > 0.05) level of fecal coliforms was found during the dry season. levels of escherichia coli, were higher (p < 0.05) during the dry season in lettuce (latuca sativa) and cilantro (coleandrum sativ ... | 1996 | 9246361 |
| specific, high affinity binding sites for an antifungal plant defensin on neurospora crassa hyphae and microsomal membranes. | hs-afp1, an antifungal plant defensin from seed of the plant heuchera sanguinea, was radioactively labeled using t-butoxycarbonyl-[35s]l-methionine n-hydroxysuccinimidyl ester, resulting in a 35s-labeled peptide with unaltered antifungal activity. [35s]hs-afp1 was used to assess binding on living hyphae of the fungus neurospora crassa. binding of [35s]hs-afp1 was found to be competitive, reversible, and saturable with an apparent kd of 29 nm and a bmax of 1.4 pmol/mg protein. [35s]hs-afp1 also b ... | 1997 | 9405418 |
| purification of the plasma membrane ca2+-atpase from radish seedlings by calmodulin-agarose affinity chromatography | the ca2+-atpase of the plasma membrane (pm) of germinating radish (raphanus sativus l.) seeds was purified by calmodulin (cam)-affinity chromatography using a batch procedure. pm purified by aqueous two-phase partitioning was solubilized with n-dodecyl beta-d-maltoside and applied to a cam-agarose matrix. after various washings with decreasing ca2+ concentrations, the ca2+-atpase was eluted with 5 mm ethylenediaminetetraacetate (edta). the edta-eluted fraction contained about 25% of the loaded c ... | 1998 | 9490776 |
| nectar-carbohydrate production and composition vary in relation to nectary anatomy and location within individual flowers of several species of brassicaceae. | nectar-carbohydrate production and composition were investigated by high-performance liquid chromatography and enzymology in nine species from five tribes of the brassicaceae. in six species (arabidopsis thaliana (l.) heynh., brassica napus l., b. rapa l., lobularia maritima (l.) desv., raphanus sativus l., sinapis arvensis l.) that produced nectar from both lateral nectaries (associated with the short stamens) and median nectaries (outside the long stamens), on average 95% of the total nectar c ... | 1998 | 9637073 |
| involvement of cucumber mosaic cucumovirus rna2 and rna3 in viral systemic spread in radish plant. | the genetics of cucumber mosaic cucumovirus (cmv) and the pathogenicity of the virus for raphanus sativus were analyzed using pseudorecombinants constructed from the infectious transcripts of two naturally occurring strains of cucumber mosaic cucumovirus (cmv-d8 and cmv-y). cmv-d8, but not cmv-y, could cause systemic infection of the plant. viral accumulation and systemic movement in the plants was examined using immuno-tissue blot analysis, dot blot and northern blot hybridization. virus was eq ... | 1998 | 9687868 |
| evidence that the role of plant defensins in radish defense responses is independent of salicylic acid. | radish leaves contain two homologous 5-kda plant defensins which accumulate systemically upon infection by fungal pathogens (f.r.g. terras et al., 1995, plant cell 7: 573-588). here we report on the molecular cloning of the cdnas encoding the two pathogen-inducible plant defensin isoforms from radish (raphanus sativus l.) leaves. tissue-print and whole-leaf electroblot immunostaining showed that the plant defensin peptides not only accumulate at high levels at or immediately around the infection ... | 1998 | 9715534 |
| borate-rhamnogalacturonan ii bonding reinforced by ca2+ retains pectic polysaccharides in higher-plant cell walls | the extent of in vitro formation of the borate-dimeric-rhamnogalacturonan ii (rg-ii) complex was stimulated by ca2+. the complex formed in the presence of ca2+ was more stable than that without ca2+. a naturally occurring boron (b)-rg-ii complex isolated from radish (raphanus sativus l. cv aokubi-daikon) root contained equimolar amounts of ca2+ and b. removal of the ca2+ by trans-1,2-diaminocyclohexane-n,n,n',n'-tetraacetic acid induced cleavage of the complex into monomeric rg-ii. these data su ... | 1999 | 9880361 |
| inhibition of growth of escherichia coli o157:h7 in fresh radish (raphanus sativus l.) sprout production by calcinated calcium. | the inhibitory effect of calcinated calcium on the growth of escherichia coli o157:h7 during fresh radish (raphanus sativus l.) sprout production was studied. it was revealed that the addition of 0.4% (wt/vol) calcinated calcium into radish sprouting medium which was artificially contaminated with e. coli o157:h7 (3.0 to 3.2 log cfu/ml) completely inhibited the growth or inactivated the microorganism. when radish seed extract was used instead of radish sprout production, the same extent of growt ... | 1999 | 10030630 |
| identification and expression of the kosena radish (raphanus sativus cv. kosena) homologue of the ogura radish cms-associated gene, orf138. | a cms-associated gene, orf125, present in the japanese radish cultivar kosena, has a sequence homologous to that of the ogura cms-associated gene, orf138, except for two amino acid substitutions and a 39 bp deletion in the orf138 coding region. in kosena radish, orf125 is linked with orfb, whereas the orf125 locus differs in a brassica napus cms cybrid derived from protoplast fusion between kosena radish and b. napus. a novel mtdna sequence is present in the 3'-flanking region of orf125 in the b ... | 1999 | 10080721 |
| an unusual case of 'uncompetitive activation' by ascorbic acid: purification and kinetic properties of a myrosinase from raphanus sativus seedlings. | myrosinase (thioglucoside glucohydrolase; ec 3.2.3.1) is a plant enzyme that hydrolyses glucosinolates, principally to isothiocyanates. myrosinase was purified to homogeneity in good yield from 8-day-old seedlings of raphanus sativus (daikon) using a four-step procedure involving chromatographies on anion exchange, hydrophobic phenyl-sepharose, gel filtration and concanavalin a-sepharose. in order to stabilize the enzyme and to avoid excessive peak broadening during chromatography, 30% (v/v) gly ... | 1999 | 10417337 |
| predicting variability in biological control of a plant-pathogen system using stochastic models. | a stochastic model for the dynamics of a plant-pathogen interaction is developed and fitted to observations of the fungal pathogen rhizoctonia solani (kühn) in radish (raphanus sativus l.), in both the presence and absence of the antagonistic fungus trichoderma viride (pers ex gray). the model incorporates parameters for primary and secondary infection mechanisms and for characterizing the time-varying susceptibility of the host population. a parameter likelihood is developed and used to fit the ... | 1999 | 10518323 |
| permeabilization of fungal membranes by plant defensins inhibits fungal growth. | we used an assay based on the uptake of sytox green, an organic compound that fluoresces upon interaction with nucleic acids and penetrates cells with compromised plasma membranes, to investigate membrane permeabilization in fungi. membrane permeabilization induced by plant defensins in neurospora crassa was biphasic, depending on the plant defensin dose. at high defensin levels (10 to 40 microm), strong permeabilization was detected that could be strongly suppressed by cations in the medium. th ... | 1999 | 10584003 |
| antiurolithiatic activity of raphanus sativus aqueous extract on rats. | the aqueous extract of the bark of raphanus sativus was tested for its antiurolithiatic and diuretic activity. the urolithiasis was experimentally induced by implantation of zinc disc in the urinary bladder of rats. significant decrease in the weight of stones was observed after treatment in animals which received aqueous extract in comparison with control groups. this extract showed an increase in the 24 h urine volume as compared to the control. | 1999 | 10624898 |
| purification and characterisation of epithiospecifier protein from brassica napus: enzymic intramolecular sulphur addition within alkenyl thiohydroximates derived from alkenyl glucosinolate hydrolysis. | epithiospecifier protein (esp), a ferrous ion dependent protein, has a potential role in regulating the release of elemental sulphur, nitriles, isothiocyanates and cyanoepithioalkanes from glucosinolates. two classes of esp polypeptides were purified with molecular masses of 39 and 35 kda, and we show that the previously reported instability was conditionally dependent. the 39 kda polypeptide was made up of two distinct isozymes (5.00, 5.14) whilst several were present for the 35 kda form of esp ... | 2000 | 10692595 |
| rescuing activity of galactoglycerolipids from cellular lesions induced by 5-aminolevulinic acid. | an anti-oxygen radical reagent of a bacterial metabolite, m874 monogalactoglycerolipid (di-o-12-methyl-tetradecanoyl-3-o-beta-d-galactopyranosyl-sn-glycerol ), was tested for its ability to protect two organisms against cellular lesions induced by 5-aminolevulinic acid (ala) and light. in corynebacterium flavescens atcc 10340, extracellular uroporphyrin and coproporphyrin were the main porphyrin products. although less than 2 mm ala increased porphyrin synthesis, ala levels above 3 mm inhibited ... | 2000 | 10788790 |
| content of glucosinolates in cruciferous vegetables grown at the same site for two years under different climatic conditions. | individual glucosinolates (gls) were determined in vegetables of three cruciferae species: brassica oleracea l. (white cabbage, red cabbage, savoy cabbage, brussels sprouts, cauliflower, kale, kohlrabi), brassica rapa l. (turnip), and raphanus sativus l. (red radish, black radish, and white radish) produced in two years. the cultivars were compared for the contents of total-, indole-, and aliphatic gls. in both years, the total content of gls was highest in black radish, and all examined r. sati ... | 2000 | 10898637 |
| chromatographic separation of a small subunit (psbw/psay) and its assignment to photosystem i reaction center. | by using a hydroxyapatite column, the five major photosystem i (psi) subunits (psaa,-b,-c,-d,-e) solubilized by sodium dodecyl sulfate (sds) were fractionated from a spinach psi reaction center preparation. another small (5-6 kda) polypeptide was also separated, and purified to homogeneity. mass spectroscopy yielded its molecular weight to be 5942 +/- 10. this polypeptide had an n-terminal sequence homologous to those of previously reported 5-kda subunits from spinach and wheat and a 6.1-kda sub ... | 2000 | 10924904 |
| antibacterial and allelopathic activity of extract from castanea sativa leaves. | following the extraction of castanea sativa with an aqueous solution of sulfuric acid (ph 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. the extract was shown to have pronounced antibacterial effects against seven of the eight strains of gram-positive and gram-negative bacteria used (mic in the range of 64-256 microg/ml and mbc in the range of 256-512 microg/ml). the active fraction was analyzed by tlc and hplc showing the presence of rutin, ... | 2000 | 10930721 |
| purification, properties, and molecular cloning of a novel ca(2+)-binding protein in radish vacuoles. | to understand the roles of plant vacuoles, we have purified and characterized a major soluble protein from vacuoles of radish (raphanus sativus cv tokinashi-daikon) taproots. the results showed that it is a novel radish vacuole ca(2+)-binding protein (rvcab). rvcab was released from the vacuolar membrane fraction by sonication, and purified by ion exchange and gel filtration column chromatography. rvcab is an acidic protein and migrated on sodium dodecyl sulfate-polyacrylamide gel with an appare ... | 2000 | 11080284 |
| regulation by organic acids of polysaccharide-mediated microbe-plant interactions. | a polysaccharide flocculant of klebsiella pneumoniae h12 has been suggested to mediate microbe-plant interactions with the aid of ca2+ [k. nakata et al., biosci. biotechnol. biochem., 64, 459-465, 2000]. here, two-way regulation of polysaccharide-mediated interactions between k. pneumoniae and raphanus sativus was studied using organic acids. namely, 10 mm equivalents of organic acids promoted production of the polysaccharide by the bacterium, but inhibited flocculation of bacterial cells by the ... | 2000 | 11129573 |
| tissue printing for myrosinase activity in roots of turnip and japanese radish and horseradish: a technique for localizing myrosinases. | a method for analyzing the tissue distribution of myrosinase activity in brassicaceous plants was developed. this technique is based on 'tissue printing' to visualize enzyme activity. the freshly-cut surface (transverse direction) of the root of three species, japanese radish (raphanus sativus), turnip (brassica campestris) and japanese horseradish (wasabi, wasabia japonica), was pressed onto a polyvinylidene difluoride (pvdf) filter to immobilize the proteins onto the membrane. the sites of myr ... | 2001 | 11166428 |
| [effect of azacytidine on expression of traits concomitant with tumor formation in the radish (raphanus sativus) in vitro]. | the effect of azacytidine, a demethylating agent, on the expression of traits concomitant with tumor formation was studied in inbred radish (rhaphanus sativus) lines carrying genomic sequences homologous to the tmr/tml genes of agrobacteriium tumefaciens. azac was found to have no effect on the traits studied, which provided evidence that the capacity for tumor formation in radish lines does not depend on the level of methylation of these sequences. | 2000 | 11190484 |
| allozyme diversity and population structure of japanese and korean populations of wild radish, raphanus sativus var. hortensis f. raphanistroides (brassicaceae). | raphanus satiuus var. hortensis f. raphanistroides (wild radish: brassicaceae) is an insect-pollinated wild plant that grows mainly on beaches in east asia. starch gel electrophoresis was used to investigate the allozyme diversity and genetic structure of 25 japanese and 9 korean populations of this plant. although the korean populations were small, isolated, and patchily distributed, they maintained a high level of genetic diversity; the average percentage of polymorphic loci was 63.1%, the mea ... | 2001 | 11376547 |
| effect of flue gas desulfurization residue on plant establishment and soil and leachate quality. | effects on soil quality and crop establishment after incorporation of flue gas desulfurization by-product (fgd) into soil as an amendment was assessed in a mesocosm study. mesocosm units received applications equivalent to 0, 2.5, 5.0, 7.5, and 10% fgd residue [0, 25, 50, 75, and 100 tons acre(-1)]. germination, biomass production, and elemental composition of corn (zea mays l. var. dekalb dk-683), soybean [glycine max (l.) merr. var. haskell pupa 94], radish (raphanus sativus l. var. sparkler), ... | 2013 | 11401255 |
| phototropism involves a lateral gradient of growth inhibitors, not of auxin. a review. | during phototropic curvature, indolyl-3-acetic acid (iaa) remains evenly distributed in the hypocotyl of sunflower (helianthus annuus l.) and in the oat (avena sativa l.) coleoptile. at the irradiated side, growth inhibiting substances accumulate. in sunflower, basipetal movement of a growth factor is not involved, since the top of the seedling can be covered or removed without affecting the photo-tropic response; this response, moreover, is independent of the rate of elongation growth. the chem ... | 1989 | 11541033 |
| transgenic radish (raphanus sativus l. longipinnatus bailey) by floral-dip method--plant development and surfactant are important in optimizing transformation efficiency. | transgenic radish (raphanus sativus l. longipinnatus bailey) plants were produced from the progeny of plants which were dipped into a suspension of agrobacterium carrying both the beta-glucuronidase (gusa) gene and a gene for resistance to the herbicide basta (bar) between t-dna border sequences. the importance of development of the floral-dipped plant and presence of surfactant in the inoculation medium were evaluated in terms of transgenic plant production. plants dipped at the primary bolt st ... | 2001 | 11592715 |
| potential allelochemicals from sambucus nigra. | twenty-four aromatic metabolites belonging to cyanogenins, lignans, flavonoids, and phenolic glycosides were obtained from sambucus nigra. structures were determined on the basis of their spectroscopic features. two compounds have been isolated and identified as (2s)-2-o-beta-d-glucopyranosyl-2-hydroxyphenylacetic acid and benzyl 2-o-beta-d-glucopyranosyl-2,6-dihydroxybenzoate. all the compounds have been assayed on dicotyledons lactuca sativa (lettuce) and raphanus sativus (radish) and monocoty ... | 2001 | 11730871 |
| 4-(methylthio)-3-butenyl isothiocyanate, a principal antimutagen in daikon (raphanus sativus; japanese white radish). | the antimutagenic activity of n-hexane extracts from eight strains of daikon (raphanus sativus; japanese white radish) have been examined using the uv-induced mutation assay of escherichia coli b/r wp2. a correlation was found between the potency of antimutagenicity and the amount of 4-(methylthio)-3-butenyl isothiocyanate (mtbitc) in their n-hexane extracts. because the pure mtbitc also showed antimutagenicity, mtbitc is presumably the active antimutagen principle in n-hexane extracts of daikon ... | 2001 | 11743759 |
| assessing potential risks from exposure to natural uranium in well water. | over 50% of the wells in the nambe region of northern new mexico exceed the us environmental protection agency's recommended drinking water standard of 20 microg l(-1) for 238u; the highest in the area was measured at 1,200 microg u l(-1). uranium uptake was estimated in tomato (lycopersicon esculentum), squash (cucurbita pepo), lettuce (lactuca scarriola), and radish (raphanus sativus) irrigated with nambe well water containing <1, 150, 500, and 1,200 microg u l(-1). plant uptake and human dose ... | 2002 | 11848150 |
| chemistry and biology of phototropism-regulating substances in higher plants. | most people are familiar with the sight of a young seedling bending towards a window or the brightest source of light to which it is exposed. this directional growth response is known as phototropism, which is caused by a lateral growth-promoting auxin in the bending organ (cholodny-went theory, cited in high school textbook). recently, however, bruinsma et al., weiler et al., and hasegawa et al. independently found that the shaded half did not contain more auxin than the illuminated one. instea ... | 2001 | 11933243 |
| the contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (daucus carota l.) potato (solanum tuberosum l.), onion (allium cepa l.) two varieties of radish (raphanus sativus l.) and gentiana purpurea l. plants. ether-soluble lipids of leaves contain 2.8-5.2%, those of storage tissue 3.1-8.9% sterol derivatives. the fractionation of lettuce (lactuca sativa l., burpee's r ... | 1970 | 11945480 | |
| transgenic expression in arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. | we developed a method for expression in arabidopsis of a transgene encoding a cleavable chimeric polyprotein. the polyprotein precursor consists of a leader peptide and two different antimicrobial proteins (amps), dmamp1 originating from dahlia merckii seeds and rsafp2 originating from raphanus sativus seeds, which are linked by an intervening sequence ("linker peptide") originating from a natural polyprotein occurring in seed of impatiens balsamina. the chimeric polyprotein was found to be clea ... | 2002 | 11950983 |
| potential of a novel antibiotic, 2-methylheptyl isonicotinate, as a biocontrol agent against fusarial wilt of crucifers. | screening for newer bioactive compounds from microbial metabolites resulted in the isolation of a novel antibiotic from the culture filtrate, streptomyces sp 201, of a soil. the bioactive compound, with antifungal and antibacterial activity, was identified as 2-methylheptyl isonicotinate. the antifungal activity of live culture, culture broth and the isolated bioactive compound showed marked inhibition against dominant soil-borne phytopathogens such as fusarium oxysporum schlect, f moniliforme s ... | 2002 | 11975177 |
| [production and cytogenetics of hybrids of ogura cms brassica campestris var. purpuraria x raphanus sativus x brassica napus]. | crosses of ogura cms brassica campestris var. purpuraria x raphanus sativus x brassica napus were made and four hybrids were produced. one plant (prn-1) was mosaic with yellow and milk white flowers and some flowers had both yellow and white petals. the others (prn-2, -3, -4) had white flowers. prn-4 had degenerated anthers, the other three had three to six anthers and could produce some pollens, but the pollens of prn-2 were unstainable by i2-ki solution. prn-2 had four normal honey glands, prn ... | 2002 | 12043578 |
| vernalization, photoperiod and ga3 interact to affect flowering of japanese radish (raphanus sativus chinese radish jumbo scarlet). | raphanus sativus l. chinese radish jumbo scarlet has characteristics that make it an excellent plant model for vernalization studies. this study further characterizes flower induction of r. sativus chinese radish jumbo scarlet. seed were imbibed in distilled water containing 0, 10-5 m or 10-3 m ga3 for 24 h and were then exposed to 6 +/- 0.5 degrees c (vernalized) for 0, 2, 4, 6, or 8 days. seedlings were then grown under a short- (8 h) or long-day photoperiod (8 h with or without a 4-h night in ... | 2002 | 12060249 |
| a study of the phylogeny of brassica rapa, b. nigra, raphanus sativus, and their related genera using noncoding regions of chloroplast dna. | there are two evolutionary lineages in the genus brassica: the rapa/oleracea lineage and the nigra lineage. using nuclear dna sequences such as the intergenic spacer between 5s rrna genes and the internal transcribed spacer between 18s and 25s rrna genes, we and others had previously demonstrated that raphanus sativus is closely related to the nigra lineage. in the present study, we sequenced the chloroplast noncoding region between trnt and trnf and that between trnd and trnt in seven species a ... | 2002 | 12069556 |
| fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane h+-atpase (iii. is there a direct interaction between the fusicoccin receptor and the plasma membrane h+-atpase?). | a radioimmunoassay using antibodies raised against bovine serum albumin-conjugated fusicoccin (fc) was applied to measure fc bound to the plasma membrane (pm) isolated from seedlings of radish (raphanus sativus l.) and of arabidopsis thaliana treated in vivo plus or minus the toxin. fc bound to the pm from seedlings treated with 5 [mu]m fc was 2-fold (radish) to 7-fold (a. thaliana) higher than the binding capacity of control pm. fc binding depended on the duration of the in vivo treatment but w ... | 1996 | 12226232 |
| comparative studies on ferredoxin-nadp+ oxidoreductase isoenzymes derived from different organs by antibodies specific for the radish root- and leaf-enzymes. | determination of the prosthetic group and titration of sulfhydryl group of ferredoxin-nadp+ oxidoreductase (fnr) from roots of radish (raphanus sativus var acanthiformis cv miyashige) confirmed its similarity to leaf-fnr. antisera directed against radish root-fnr and leaf-fnr distinguished the enzyme forms from roots and leaves of radish as well as other flowering plants. the fnr isoenzymes showed organ-specific distributions. in horsetail (equisetum arvense l.) and cultured liverwort cells (mar ... | 1993 | 12231952 |
| synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape 2s albumins and by barley trypsin inhibitors. | although thionins and 2s albumins are generally considered as storage proteins, both classes of seed proteins are known to inhibit the growth of pathogenic fungi. we have now found that the wheat (triticum aestivum l.) or barley (hordeum vulgare l.) thionin concentration required for 50% inhibition of fungal growth is lowered 2- to 73-fold when combined with 2s albumins (at sub- or noninhibitory concentrations) from radish (raphanus sativus l.) or oilseed rape (brassica napus l.). furthermore, t ... | 1993 | 12232024 |
| nmr solution structure of attp, an arabidopsis thaliana trypsin inhibitor. | the three-dimensional structure of the precursor form of the arabidopsis thaliana trypsin inhibitor (att(p), genbank entry z46816), a 68-residue (approximately 7.5 kda) rapeseed class proteinase inhibitor, has been determined in solution at ph 5.0 and 25 degrees c by multinuclear magnetic resonance spectroscopy. the protein contains one alpha-helix and two strands of antiparallel beta-sheet, with a type iv beta-turn connecting the two strands. the alpha-helix and the inhibitory loop are connecte ... | 2002 | 12369816 |
| effects of black radish root (raphanus sativus l. var niger) on the colon mucosa in rats fed a fat rich diet. | the effect of black radish root (raphanus sativus l. var niger) was studied on the structure and redox state of the colon mucosa in fat-rich diet fed rats. the epithelial lining disrupted, the number of enterocytes and the goblet cells reduced and inflammatory cells were observed in rats fed with a fat-rich diet. after treatment with granules from black radish root all of the histopathological changes and parameters of the redox state caused by the fat-rich diet were improved. the structure of t ... | 2002 | 12410553 |
| effects of oxidizing adulterants on detection of 11-nor-delta9-thc-9-carboxylic acid in urine. | bleach, nitrite, chromate, and hydrogen peroxide-peroxidase are effective urine adulterants used by the illicit drug users to conceal marijuana-positive results. methods for detecting nitrite and chromate are available. effects of other oxidizing agents that could possibly be used as adulterants and are difficult to detect or measure are presented in this report. urine samples containing 40 ng/ml of 11-nor-delta9-thc-9-carboxylic acid (thc-acid) were treated with 10 mmol/l of commonly available ... | 2002 | 12423000 |
| reducing environmental bias when measuring natural selection. | crucial to understanding the process of natural selection is characterizing phenotypic selection. measures of phenotypic selection can be biased by environmental variation among individuals that causes a spurious correlation between a trait and fitness. one solution is analyzing genotypic data, rather than phenotypic data. genotypic data, however, are difficult to gather, can be gathered from few species, and typically have low statistical power. environmental correlations may act through traits ... | 2002 | 12487346 |
| equilibrium dialysis measurements of the ca2+-binding properties of recombinant radish vacuolar ca2+-binding protein expressed in escherichia coli. | vacuoles of radish (raphanus sativus) contained a ca2+-binding protein (rvcab) of 43 kda. we investigated the ca2+-binding properties of the protein. rvcab was expressed in escherichia coli and was purified from an extract by ion-exchange chromatography, nitrocellulose membrane filtration, and gel-filtration column chromatography. ca2+-binding properties of the recombinant protein were examined by equilibrium dialysis with 45ca2+ and small dialysis buttons. the protein was estimated to bind 19ca ... | 2002 | 12506976 |
| the effects of soil amendments on heavy metal bioavailability in two contaminated mediterranean soils. | two heavy metal contaminated calcareous soils from the mediterranean region of spain were studied. one soil, from the province of murcia, was characterised by very high total levels of pb (1572 mg kg(-1)) and zn (2602 mg kg(-1)), whilst the second, from valencia, had elevated concentrations of cu (72 mg kg(-1)) and pb (190 mg kg(-1)). the effects of two contrasting organic amendments (fresh manure and mature compost) and the chelate ethylenediaminetetraacetic acid (edta) on soil fractionation of ... | 2003 | 12531318 |
| identification and classification of s haplotypes in raphanus sativus by pcr-rflp of the s locus glycoprotein (slg) gene and the s locus receptor kinase (srk) gene. | polymorphism of the s-locus glycoprotein (slg) and s-locus receptor kinase (srk) genes in raphanus sativus was analyzed by pcr-rflp using slg- and srk-specific primers. twenty four inbred lines of r. sativus could be grouped into nine s haplotypes. dna fragments of slg alleles specifically amplified from five s haplotypes by pcr with class-i slg-specific primers showed different profiles upon polyacrylamide-gel electrophoresis after digestion with restriction endonucleases. the five r. sativus s ... | 2002 | 12582578 |
| isolation of phytotoxic compounds from tree-of-heaven (ailanthus altissima swingle). | the aqueous root extract of ailanthus altissima showed allelopathic activity against radish (raphanus sativus l. cv. "saxa"), garden cress (lepidium sativum l.), and purslane (portulaca oleracea l.) seeds. a bioassay-oriented purification of active extracts, chromatographic fractions, and compounds demonstrated dose-dependent activity on germination and radicle growth of test seeds; radish seed was the most sensitive to allelochemicals. active compounds have been isolated: ailanthone, ailanthino ... | 2003 | 12590453 |
| toxicity of copper in sewage sludge. | sewage sludge is a source of organic matter and nutrients, but a major obstacle for its recycling is that the municipal wastewater sludge has low but significant levels of contaminants. this investigation, on the acute toxicity of copper in sewage sludge, was conducted with three organisms, daphnia magna, lemna minor and raphanus sativus (seeds). the toxicity of the leakage water from sewage sludge spiked with cuso(4) was studied for 64 days. the toxicity increased during the first 8-16 days and ... | 2003 | 12605925 |
| inhibition of mutagenicity of food-derived heterocyclic amines by sulphoraphene--an isothiocyanate isolated from radish. | the naturally derived isothiocyanate, sulphoraphene [4-isothiocyanato-(1r)-(methylsulphinyl)-1-(e)-butene], isolated from seeds of radish ( raphanus sativus l., cruciferae) was investigated for its antigenotoxic effects against a battery of cooked food mutagens (heterocyclic amines) in the ames salmonella/reversion assay using salmonella typhimurium ta98 (frame-shift mutation sensitive) and ta100 (base -pair mutation sensitive) bacterial strains in the presence of aroclor 1254 induced rat liver ... | 2003 | 12624832 |
| "chitin-specific" peroxidases in plants. | the activity of various plant peroxidases and the ability of their individual isoforms to bind chitin was studied. some increase in peroxidase activity was observed in crude extracts in the presence of chitin. activated peroxidases of some species fell in the fraction not sorbed on chitin and those of other species can bind chitin. only anionic isoperoxidases from oat (avena sativa), rice (oryza sativa), horseradish (armoracia rusticana), garden radish (raphanus sativus var. radicula), peanut (a ... | 2003 | 12693984 |
| delimitation of the fertility restorer locus rfk1 to a 43-kb contig in kosena radish (raphanus sativus l.). | we are pursuing a positional cloning strategy to isolate the fertility restoration gene rfk1 from radish. random polymorphic dna-sequence-tagged site (rapd-sts) markers tightly linked to the gene in radish were isolated, and a rapd map surrounding the rfk1 locus was constructed. we surveyed 948 f2 plants with adjacent rapd-sts markers to isolate recombinants for bulk segregant analysis. this analysis was effective in isolating tightly linked amplification fragment length polymorphism (aflp) mark ... | 2003 | 12712328 |
| identification of the fertility restoration locus, rfo, in radish, as a member of the pentatricopeptide-repeat protein family. | ogura cytoplasmic male sterility (cms) in radish (raphanus sativus) is caused by an aberrant mitochondrial gene, orf138, that prevents the production of functional pollen without affecting female fertility. rfo, a nuclear gene that restores male fertility, alters the expression of orf138 at the post-transcriptional level. the ogura cms/rfo two-component system is a useful model for investigating nuclear-cytoplasmic interactions, as well as the physiological basis of fertility restoration. using ... | 2003 | 12740605 |
| effects of pulp mill solids and three composts on early growth of tomatoes. | compost has been proposed as a means of simultaneously diverting organic materials from landfills while producing a valuable product that improves tilth, organic matter content and nutrient supply of agricultural soils. composts manufactured from different source materials may have markedly different properties however, even if they meet all regulatory requirements. we compared the capacity of composts made from three different combinations of organic wastes (horse manure and bedding, mink farm ... | 2003 | 12798121 |
| phytotoxicity to and uptake of enrofloxacin in crop plants. | phytotoxicity of enrofloxacin on crop plants cucumis sativus, lactuca sativa, phaseolus vulgaris and raphanus sativus was determined in a laboratory model: the effect of 50, 100 and 5000 microgl(-1) were evaluated after 30 days exposure by measuring post-germinative growth of primary root, hypocotyl, cotyledons and leaves. concentrations between 50 and 5000 microgl(-1) induced both toxic effect and hormesis in plants, by significantly modifying both length of primary root, hypocotyl, cotyledons ... | 2003 | 12821004 |
| somatic hybrids between arabidopsis thaliana and cytoplasmic male-sterile radish (raphanus sativus). | somatic hybrids were produced by protoplast fusion between arabidopsis thaliana ecotype columbia and a male-sterile radish line ms-gensuke ( raphanus sativus) with the ogura cytoplasm. forty-one shoots were differentiated from the regenerated calli and established as shoot cultures in vitro. about 20 of these shoots were judged to be hybrids based on growth characteristics and morphology. molecular analyses of 11 shoots were performed, confirming the hybrid features. of these 11 shoots, eight we ... | 2003 | 12827437 |
| growth stimulation of ectomycorrhizal fungi by root exudates of brassicaceae plants: role of degraded compounds of indole glucosinolates. | brassicaceae plants are nonmycorrhizal. they were found to inhibit va mycorrhizal infection in their host plants. we tested if they can influence growth of ectomycorrhizal (ecm) fungi. when roots and leaves of brassicaceae plants and ecm fungi were cultured together in the same petri dishes, the root exudates of turnip (brassica rapa), swede (b. napobrassica), cabbage (b. oleracea, var. capitata), broccoli (b. oleracea, var. italica plenck), kohlrobi (b. caulorapa pasq.), mustard (b. juncea), ra ... | 2003 | 12918920 |
| [antimicrobial properties of raphanus sativus. ii. antimicrobial properties of a preparation from acid substances from raphanus sativus var. niger]. | 1961 | 14478783 | |
| essential oils from mediterranean lamiaceae as weed germination inhibitors. | the essential oils obtained from rosemary (rosmarinus officinalis l.), thyme (thymus vulgaris l.), and savory (satureja montana l.) and the four monoterpenes that are their major constituents have been analyzed by gc and gc-ms and tested for their allelopathic properties on the seeds of three different annual weeds (chenopodium album, portulaca oleracea, and echinochloa crus-galli) and three crops (raphanus sativus, capsicum annuum, and lactuca sativa), with the aim to evaluate in vitro their po ... | 2003 | 14518938 |
| defensins from insects and plants interact with fungal glucosylceramides. | growth of the yeast species candida albicans and pichia pastoris is inhibited by rsafp2, a plant defensin isolated from radish seed (raphanus sativus), at micromolar concentrations. in contrast, gcs-deletion mutants of both yeast species are resistant toward rsafp2. gcs genes encode udp-glucose:ceramide glucosyltransferases, which catalyze the final step in the biosynthesis of the membrane lipid glucosylceramide. in an enzyme-linked immunosorbent assay-based binding assay, rsafp2 was found to in ... | 2004 | 14604982 |
| antioxidant constituents of radish sprout (kaiware-daikon), raphanus sativus l. | methanol extracts of 11 kinds of commonly available vegetables were examined for hydroxyl radical scavenging potency using the bleomycin-fe method. in this method, the iron ion and bleomycin in water form hydroxyl radicals, and the scavenging activity is monitored by the modified thiobarbituric acid method. all extracts showed scavenging capacity, even though the activity of some of them was lower than that of l-ascorbic acid. those vegetables were classified into three groups according to their ... | 2003 | 14690397 |
| [antibiotic effect of the allyl isothiocyanate extracted from various horticultural forms of the seeds of raphanus sativus l., var. radicula pers. towards various bacteria, including two strains of tubercle bacillus avian type, cow 18 and cow 70; note ii]. | 1951 | 14864944 | |
| transfer of resistance against the beet cyst nematode from radish (raphanus sativus) to rape (brassica napus) by monosomic chromosome addition. | in rape ( brassica napus), no resistance to the beet cyst nematode (bcn) heterodera schachtii is available. this study was carried out to determine the specific chromosome(s) of resistant radish ( raphanus sativus) carrying the gene(s) for nematode resistance as a prequisite to convert rape from a host into a trap crop for this pest. a raphanobrassica progeny of 25 plants was analyzed which segregated for all nine chromosomes of the raphanus genome in a genetic background of synthetic rape. the ... | 2004 | 14991110 |
| tissue-specific expression and developmental regulation of cytochrome b561 genes in arabidopsis thaliana and raphanus sativus. | ascorbate (asc) is an essential molecule in many aspects of development and stress responses in plants and animals. cytochromes b561 (cyts b561) are tightly coupled to asc homeostasis. these proteins are found in mammalian tissues, where they are involved in the regeneration of asc, serving the synthesis of catecholamine neurotransmitters, and in intestinal iron reduction. plant genomes encode homologous membrane-associated, asc-reducible cyts b561. the expression of these proteins in plants, ho ... | 2004 | 15032866 |
| uptake and translocation of phytochemical 2-benzoxazolinone (boa) in radish seeds and seedlings. | the molecular aspects of phytochemical interactions between plants, especially the process of phytochemical translocation by the target plant, remain challenging for those studying allelopathy. 2-benzoxazolinone (boa) is a natural chemical produced by rye (secale cereale) and is known to have phytotoxic effects on weed seeds and seedlings. the translocation of boa into target plants has been poorly investigated. therefore, the total absorption of [ring u 14c] boa was estimated by oxidizing whole ... | 2004 | 15181106 |
| an important determinant of the ability of turnip mosaic virus to infect brassica spp. and/or raphanus sativus is in its p3 protein. | turnip mosaic virus (tumv, genus potyvirus, family potyviridae) infects mainly cruciferous plants. isolates tu-3 and tu-2r1 of tumv exhibit different infection phenotypes in cabbage (brassica oleracea l.) and japanese radish (raphanus sativus l.). infectious full-length cdna clones, ptuc and ptur1, were constructed from isolates tu-3 and tu-2r1, respectively. progeny virus derived from infections with ptuc induced systemic chlorotic and ringspot symptoms in infected cabbage, but no systemic infe ... | 2004 | 15218194 |
| variation of female frequency and cytoplasmic male-sterility gene frequency among natural gynodioecious populations of wild radish (raphanus sativus l.). | in gynodioecious plant populations, sex determination often involves both cytoplasmic male-sterility (cms) genes and specific nuclear genes that restore male function. how gynodioecy is maintained under the joint dynamics of cms and restorer genes remains controversial. although many theoretical models deal with interactions between cms genes and restorer genes with sexual phenotypes and predict changes in their frequencies, it is difficult to observe the frequencies because no molecular markers ... | 2004 | 15245417 |
| phytoextraction of heavy metals by canola (brassica napus) and radish (raphanus sativus) grown on multicontaminated soil. | phytoextraction can provide an effective in situ technique for removing heavy metals from polluted soils. the experiment reported in this paper was undertaken to study the basic potential of phytoextraction of brassica napus (canola) and raphanus sativus (radish) grown on a multi-metal contaminated soil in the framework of a pot-experiment. chlorophyll contents and gas exchanges were measured during the experiment; the heavy metal phytoextraction efficiency of canola and radish were also determi ... | 2004 | 15276270 |
| water channel activity of radish plasma membrane aquaporins heterologously expressed in yeast and their modification by site-directed mutagenesis. | plants contain a number of aquaporin isoforms. we developed a method for determining the water channel activity of individual isoforms of aquaporin. six plasma membrane aquaporins (rspips) and two vacuolar membrane aquaporins (rstips) of radish (raphanus sativus) were expressed heterologously in saccharomyces cerevisiae bj5458, which is deficient in endogenous functional aquaporin. aquaporins were detected by immunoblot analysis with corresponding antibodies. water permeability of membranes from ... | 2004 | 15295065 |
| differential antifungal and calcium channel-blocking activity among structurally related plant defensins. | plant defensins are a family of small cys-rich antifungal proteins that play important roles in plant defense against invading fungi. structures of several plant defensins share a cys-stabilized alpha/beta-motif. structural determinants in plant defensins that govern their antifungal activity and the mechanisms by which they inhibit fungal growth remain unclear. alfalfa (medicago sativa) seed defensin, msdef1, strongly inhibits the growth of fusarium graminearum in vitro, and its antifungal acti ... | 2004 | 15299136 |
| bayesian analysis of botanical epidemics using stochastic compartmental models. | a stochastic model for an epidemic, incorporating susceptible, latent, and infectious states, is developed. the model represents primary and secondary infection rates and a time-varying host susceptibility with applications to a wide range of epidemiological systems. a markov chain monte carlo algorithm is presented that allows the model to be fitted to experimental observations within a bayesian framework. the approach allows the uncertainty in unobserved aspects of the process to be represente ... | 2004 | 15302941 |
| occurrence of gluconacetobacter diazotrophicus in tropical and subtropical plants of western ghats, india. | endophytic bacteria were isolated from the tissues of surface sterilized roots, stems, and leaves of fifty different crop plants. phenotypic, biochemical tests and species-specific pcr assay permitted identification of four isolates of gluconacetobacter diazotrophicus from root tissues of carrot (daucus carota l.), raddish (raphanus sativus l.), beetroot (beta vulgaris l.) and coffee (coffea arabica l.). further the plant growth promoting traits such as nitrogenase activity, production of phytoh ... | 2004 | 15462523 |
| pharmacological basis for the gut stimulatory activity of raphanus sativus leaves. | the crude extract of raphanus sativus leaves (rl.cr) showed a dose-dependent (0.03-5.0 mg/ml) spasmogenicity in guinea-pig ileum and colon. the effect was insensitive to atropine pre-treatment but was completely abolished by pyrilamine indicating involvement of histaminergic (h(1)) receptors. the contractile effect at high doses (3.0-5.0mg/ml) was followed by relaxation. rl.cr also enhanced the transit of charcoal meal in mice at 30-100 mg/kg. the petroleum spirit, chloroform and aqueous fractio ... | 2004 | 15507331 |
| diversification and alteration of recognition specificity of the pollen ligand sp11/scr in self-incompatibility of brassica and raphanus. | the recognition specificity of the pollen ligand of self-incompatibility (sp11/scr) was investigated using brassica rapa transgenic plants expressing sp11 transgenes, and sp11 of raphanus sativus s-21 was found to have the same recognition specificity as that of b. rapa s-9. in a set of three s haplotypes, whose sequence identities of sp11 and srk are fairly high, r. sativus s-6 showed the same recognition specificity as brassica oleracea s-18 and a slightly different specificity from b. rapa s- ... | 2004 | 15548734 |
| purification and cloning of a chinese red radish peroxidase that metabolise pelargonidin and forms a gene family in brassicaceae. | an anionic peroxidase rsprx1 was purified (rz=3.0) and characterized from roots of chinese red radish (raphanus sativus var. niger, brassicaceae). the specific activity of rsprx1 (micromol mg(-1) min(-1)) is 413.5 (ferulic acid); 258.7 (abts); 177.3 (caffeic acid) and 10.0 (guaiacol acid). the optimum ph is 4.0 (citrate buffer) using abts as substrate. rsprx1 can utilise the red pigment present in the root, pelargonidin, as substrate and the specific activity is 93.6 micromol mg(-1) min(-1). the ... | 2004 | 15588587 |
| conversion of the carbohydrate structures of glycoproteins in roots of raphanus sativus using several glycosidase inhibitors. | an attempt was made to convert the n-glycan structures in raphanus sativus seeds during germination with a view to develop a method for regulating the n-glycan structures using glycosidase inhibitors. the n-glycan structures of glycoproteins in the roots of seedlings germinated for three days were analyzed by hydrazinolysis followed by n-acetylation, pyridylamination and hplc. pyridylaminated sugar chains obtained in the absence of the inhibitors had plant type structures consisting of man(3)fuc ... | 2004 | 15625323 |