Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| conditions for in vitro haemolytic activity by corynebacterium ovis exotoxin. | a substance, concluded to be corynebacterium ovis exotoxin on the basis of properties shared, was found to have haemolytic activity below ph 6 and red cell adhesion activity at neutral or slightly alkaline ph. an acidified, solid blood agar medium was used to demonstrate the extent of haemolysis that could be obtained at optimal ph and its inhibition by immune serum. culture in a liquid medium giving rise to a suitably acidic ph allowed titration for haemolytic activity by the exotoxin and use o ... | 1979 | 42127 |
| a simple method of detecting staphylococcal hemolysins. | a modification of the "one-plate method" for detection of staphylococcal hemolysins has been described. bacterial strains of staphylococcus aureus, streptococcus agalactiae and cornebacterium pseudotuberculosis were replaced by their prepurified hemolytically active exosubstances: beta-toxin, camp-factor, and, the exosubstance of corynebacterium pseudotuberculosis called corex. by means of the modification described, exact detection of hemolysins in possible with staphylococcal strains producing ... | 1979 | 44782 |
| a comparison of five serological tests for the diagnosis of experimental corynebacterium ovis infection in sheep. | 1979 | 106921 | |
| non-specific agglutination of corynebacterium ovis by precolostral and young lamb sera. | an agglutination reaction, relatively coarse compared to that obtained with immune sera, was observed when corynebacterium ovis cells were incubated with lamb sera collected before and after ingesting colostrum. the reaction did not appear to involve immunoglobulin since it was not affected by treatment of precolostral lamb serum with rivanol, mercaptoethanol or rabbit antisheep serum. igm was detected in only three of 10 precolostral samples with agglutinating activity and immunofluorescence st ... | 1978 | 107549 |
| the variable response of bacteria to excess ferric iron in host tissues. | the enhancement by exogenous ferric iron, both systemic and local, of the infectivity of 120 strains of bacteria, representing 17 genera, was measured in the skin of guinea-pigs. systemic iron enhanced only 23% of 115 strains, and local iron 49% of 71 strains. systemic iron, by an apparently anti-inflammatory action, depressed the size of lesions produced by 27 of the non-enhanced strains from nine of the genera tested. for most strains, the degree of enhancement was small, ranging from 2- to 8- ... | 1979 | 372534 |
| fluorescent antibody technique in the diagnosis of corynebacterium pseudotuberculosis. | 1978 | 373819 | |
| diagnostic availability of the hemolytically active exosubstance of corynebacterium pseudotuberculosis for isolation and identification of streptococcus agalactiae and its comparison with the beta-toxin of staphylococcus aureus. | 1979 | 393011 | |
| purification and partial characterization of the exotoxin of corynebacterium ovis. | 1. the toxin from corynebacterium ovis, a phospholipase d (sphingomyelin phosphodiesterase d) that acts on 2-lysophosphatidylcholine and sphingomyelins, was purified by about 400-fold to homogeneity as judged by several criteria. [the ec number of the toxin (ec 3.1.4.41) has been allotted by the nomenclature committee of iub, but has not yet been published.] 2. a new assay method performed in vitro, based on inhibition by the toxin of erythrocyte lysis by staphylococcal beta-haemolysin, was deve ... | 1979 | 426767 |
| pseudotuberculous adenitis caused by corynebacterium pseudotuberculosis. | 1979 | 430545 | |
| corynebacterium pseudotuberculosis. a new cause of infectious and eosinophilic pneumonia. | a 28 year old veterinary medical student experienced spiking fever, cough, peripheral blood eosinophilia and an eosinophilic pulmonary infiltrate. corynebacterium pseudotuberculosis was isolated from a transtracheal aspirate and bronchoscopy washings. c. pseudotuberculosis, a pathogen responsible for lymphadenitis in livestock, has never been reported to cause pneumonia in man. in the four cases of c. pseudotuberculosis previously reported, lymphadenitis was the chief clinical presentation. in o ... | 1979 | 463927 |
| serum proteins of normal goats and goats with caseous lymphadenitis. | values for total serum proteins and relative percentages of albumin, alpha 1-globulin, alpha 2-globulin, beta-globulin, and gamma-globulin were determined for the goat. these normal values were compared with those obtained for goats infected with corynebacterium pseudotuberculosis. goats chronically infected with c pseudotuberculosis show significantly higher total serum protein values than normal goats, apparently due to increased gamma-globulins. this higher protein value is also associated wi ... | 1979 | 475093 |
| purification of 1-monoacylglycerols containing alpha-branched-beta-hydroxylated fatty acids from lipids of corynebacterium pseudotuberculosis. | a complex mixture of monoacylglycerols, identified as (c30--c36) corynomycoloylglycerols was isolated from the diethyl ether/acetone-soluble lipids of corynebacterium pseudotuberculosis. | 1979 | 498380 |
| visceral caseous lymphadenitis in thin ewe syndrome: isolation of corynebacterium, staphylococcus, and moraxella spp from internal abscesses in emaciated ewes. | the relationship between the visceral form of caseous lymphadenitis and a chronic debilitating condition of mature sheep designated as the thin ewe syndrome was investigated. internal abscesses were found during necropsy in 81% of animals with thin ewe syndrome and corynebacterium pseudotuberculosis (c ovis) was recovered from 86% of the animals with internal abscesses. other pyogenic bacteria, including c pyogenes, c equi, staphylococcus epidermis, s aureus, and pseudomonas aeruginosa were ofte ... | 1979 | 525916 |
| pathology and bacteriology of abortion in sheep experimentally infected with corynebacterium pseudotuberculosis. | 1979 | 548139 | |
| immunological events in the popliteal lymph node of sheep following injection of liver or killed corynebacterium ovis into an afferent popliteal lymphatic duct. | efferent popliteal lymph and blood were collected daily for three weeks from sheep following injection of live or killed corynebacterium ovis organisms into an afferent lymphatic duct. the total lymphocyte output, proportion of blast cells and class specificity of immunoglobulin-containing groups of animals and antibody titres and concentrations of igg1, igg2, img and iga were measured in both lymph and blood... | 1977 | 557223 |
| effect of levamisole on immunity to corynebacterium pseudotuberculosis in mice and sheep. | the stimulating effect of levamisole on immunity to corynebacterium pseudotuberculosis in mice was marginal, while no enhancement of immunity could be detected in sheep. the conclusion was reached that, as levamisole has no potentiating effect on immunity to c. pseudotuberculosis in normal sheep, it is of no practical value as an immunostimulant in this instance. | 1977 | 614519 |
| internal abdominal abscesses in the horse: a study of 25 cases. | internal abdominal abscesses in 25 horses, including 8 horses that died or were euthanatized, were found to be caused by streptococcus equi, streptococcus zooepidemicus, or corynebacterium pseudotuberculosis. breed or sex predilection was not found. although horses of all ages were affected, those under 5 years of age were more commonly affected. nearly all of the horses had a history of respiratory catarrh or lymphadenitis. horses with internal abdominal abscesses also had intermittent, prolong ... | 1978 | 621178 |
| action of corynebacterium ovis exotoxin on endothelial cells of blood vessels. | 1978 | 622164 | |
| an indirect haemagglutination test for the sero-diagnosis of c ovis infection in sheep. | an indirect haemagglutination (iha) test for the diagnosis of caseous lymphadenitis in sheep is described; the test uses purified corynebacterium ovis toxin to sensitise formalinised sheep erythrocytes treated with bis-diazobenzidine. animals with titres of 1/16 and above were considered positive to the test. examination of sera from proven cases of caseous lymphadenitis by the iha test and anti-haemolysin-inhibition (ahi) tests showed that the iha test was more sensitive than the ahi test. | 1978 | 625601 |
| a serologic method for the detection of corynebacterium pseudotuberculosis infections in horses. | a serologic technique useful for detecting antibodies formed in horses in response to infection with corynebacterium pseudotuberculosis is described. the test relies on the ability of c. pseudotuberculosis toxin to produce a wide zone of hemolysis when applied to erythrocytes previously treated with a sterile filtrate of corynebacterium equi broth culture. the synergistic hemolytic activity can be neutralized by anti-c. pseudotuberculosis serum. this test was used to analyze sera from 616 horses ... | 1978 | 639519 |
| effect on sphingomyelin-containing liposomes of phospholipase d from corynebacterium ovis and the cytolysin from stoichactis helianthus. | the toxic, sphingomyelin-specific phospholipase d (phosphatidylcholine phosphatidohydrolase ec 3.1.4.4) from corynebacterium ovis was purified to near homogeneity. it has a molecular weight of 31 000 and a pi of approx. 9.8. although not cytolytic itself, it protected red cells from hemolysis by staphylococcal sphingomyelinase (beta-hemolysin) and helianthus toxin. the apparently non-enzymatic cytolysin (helianthus toxin) from the sea anemone stoichactis helianthus also interacts with membrane s ... | 1978 | 667093 |
| experimental induction of caseous lymphadenitis in sheep by intralymphatic inoculation of corynebacterium ovis. | lesions histologically similar to those of natural caseous lymphadenitis developed in sheep popliteal lymph nodes following injection of viable corynebacterium ovis cells into afferent lymphatic ducts. histopathological examination of affected lymph nodes suggested that exotoxin might be involved in the establishment, progressive development and persistence of caseous lymphadenitis lesions through localised cytotoxicity. | 1978 | 674838 |
| caseous lymphadenitis in sheep -- methods of infection. | depending on the route of infection, experimental exposure of 30 sheep to corynebacterium pseudotuberculosis resulted in either mucopurulent vaginitis, prosthitis, conjunctivitis, rhinitis, or in subcutaneous abscessation. caseous lymphadenitis, from which the organism could be recovered in 52 out of 56 instances, occurred in every instance with the exception of animals infected by the preputial route. | 1976 | 994136 |
| corynebacterium pseudotuberculosis as a cause of subacute interstitial nephritis in a chimpanzee. | 1976 | 1048802 | |
| the use of mouse protection tests in the diagnosis of caseous lymphadenitis in sheep. | mouse protection tests gave promising results in the diagnosis of caseous lymphadenitis in sheep especially those presenting no external lesions. the test was specific since sera of infected sheep were capable of neutralising corynebacterium ovis toxin while sera of healthy sheep failed to do so. better results were obtained with 1-0 ml than 0-5 or 0-25 ml serum of infected sheep to neutralise 2 mld of the toxin through mice. | 1975 | 1129543 |
| enhanced resistance to corynebacterium pseudotuberculosis infections associated with reduced serum immunoglobulin levels in levamisole-treated mice. | the effect of levamisole (1-2,3,5,6-tetrahydro-6-imidazo[2,1-b]thiazole monohydrochloride) treatment on the course of a corynebacterium pseudotuberculosis infection and the ability of either vaccinated or unvaccinated randomly bred mice to resist challenge were investigated. it was found that either a double treatment of levamisole administered with or without a sublethal inoculation of c. pseudotuberculosis or a single treatment of levamisole alone had a significant effect on the ability of mic ... | 1975 | 1193726 |
| comparative toxic effect of the surface lipid of corynebacterium ovis on peritoneal macrophages. | the postphagocytic effect on mouse, rabbit, and guinea pig peritoneal macrophages of a petrol-ether lipid extract from corynebacterium ovis (c. pseudotuberculosis) representing the surface coat of the organism external to the cell wall was investigated by examing three parameters of cytotoxicity, viability assayed by dye exclusion, glycolytic activity, and ultrastructural morphology. the viability test demonstrated a lethal effect on normal and immune mouse macrophages but not on those of the ra ... | 1975 | 1205621 |
| relation between the toxogenicity and pyogenicity of corynebacterium ovis in experimentally infected mice. | suspensions of corynebacterium ovis subjected to physical and chemical treatment failed to kill inoculated mice but were able to produce sterile pyogenic lesions. it appears that the exotoxin is different from the pyogenic factor and that the latter is attached to a heat-stable substance in the bacterial cell. mice immunised with antitoxin showed local non-spreading lesions when subsequenly inoculated with washed c ovis cells while unprotected control mice showed a spreading type of infection wh ... | 1976 | 1265358 |
| chronic contagious ecthyma and caseous lymphadenitis in two boer goats. | 1992 | 1287954 | |
| production of an enzymatically inactive analog of phospholipase d from corynebacterium pseudotuberculosis. | the gene pld, encoding the phospholipase d (pld) of corynebacterium pseudotuberculosis, was mutagenized using formic acid and then expressed in escherichia coli. mutagenesis was targeted at the coding region of pld, so as to produce only one or a limited number of point mutations. transformants were screened for the enzymatic and immunological properties of their pld products. one clone was found to produce a protein which was enzymatically inactive, but which was comparable to the wild-type pld ... | 1992 | 1398078 |
| ovine mononuclear phagocytes in situ: identification by monoclonal antibodies and involvement in experimental pyogranulomas. | in order to characterize in situ the macrophages present in experimental pyogranulomas induced in lambs with corynebacterium pseudotuberculosis, a set of monoclonal antibodies (mabs) was produced following immunization of balb/c mice with alveolar macrophages from healthy sheep. three mabs were retained after two steps of screening using alveolar macrophages, peripheral blood lymphocytes, and polymorphonuclear leukocytes as target cells. their reactivity was tested not only on macrophages in pyo ... | 1992 | 1431556 |
| serological response of vaccinated sheep after challenge with corynebacterium pseudotuberculosis. | 1992 | 1445082 | |
| double-antibody sandwich enzyme-linked immunosorbent assay and immunoblot analysis used for control of caseous lymphadenitis in goats and sheep. | a double-antibody sandwich elisa for detection of antibodies directed against the exotoxin of corynebacterium pseudotuberculosis, the cause of caseous lymphadenitis (cl) in small ruminants, was developed. a concentrated exotoxin was used. for interpretation of elisa results, these sera were tested: sequentially obtained sera of c pseudotuberculosis-inoculated goats and sheep that were monitored for 68 weeks; sequentially obtained sera from 80 goats of 3 flocks with cl; sera from 652 goats of 7 f ... | 1992 | 1497180 |
| analysis of the immunodominant antigens of corynebacterium pseudotuberculosis. | antibodies to seven antigens in a whole cell lysate of corynebacterium pseudotuberculosis ranging in molecular mass from 22 to 120 kilodaltons (kda) were present in sera of 40 sheep and goats infected with c. pseudotuberculosis. three antigens of about 120, 68, and 31.5 kda in size were consistently detected with sera from all animals and twenty-two sera had antibodies to 64, 43, 40, and 22 kda antigens. none of these antigens were detected by sera from 160 sheep in a c. pseudotuberculosis-free ... | 1992 | 1531563 |
| what is your diagnosis? abscess causing lameness in a horse. | 1992 | 1548177 | |
| rational attenuation of corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle. | the phospholipase d (pld) gene (pld) has been deleted from the corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. sheep infection trials indicate that the pld-negative c. pseudotuberculosis strain (toxminus) is incapable of inducing caseous lymphadentis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. this clearly establishes pld as a major c. pseudotuberculosis virulence factor. vaccination of sheep with li ... | 1992 | 1612756 |
| the effect of experimental infection with corynebacterium pseudotuberculosis on reproduction in adult ewes. | fifteen ewes were inoculated subcutaneously with 2 x 10(6) cells of corynebacterium pseudotuberculosis seven days before mating, during the embryonic stage of gestation and during the fetal stage of gestation. the clinical signs, antibody response and the consequences of the infection on reproduction were studied. none of the ewes showed any change in general condition during pregnancy. the effects of experimental infection during gestation were evident after incubation periods that ranged from ... | 1992 | 1620958 |
| hemolytic interactions of dermatophilus congolensis. | the strains of dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. in testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with rhodococcus equi and streptococcus agalactiae, whereas with staphylococcus aureus producing beta hemolysin and with staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. first of all a conspicuous inhi ... | 1992 | 1621476 |
| differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with corynebacterium pseudotuberculosis, pasteurella haemolytica, or lentiviruses. | soluble mediators such as tumor necrosis factor alpha (tnf-alpha) may be important in the pathogenesis of many chronic pulmonary infections. we examined the ability of corynebacterium pseudotuberculosis, pasteurella haemolytica, and ovine lentiviruses (ovlv) to induce tnf-alpha secretion by pulmonary alveolar macrophages (pam). bronchoalveolar lavage cells, composed of greater than 90% pam, were obtained from normal sheep. bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in ... | 1991 | 1652561 |
| antigen specificity of antibody responses to corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis. | constituents of corynebacterium pseudotuberculosis were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). analysis of sonicated whole bacterial cells and ether-extracted cells revealed more than 35 bands in silver-stained gels. sds-page analysis of concentrated culture filtrates with exotoxin activity demonstrated more than 15 bands. sera from sheep with c. pseudotuberculosis-induced disease of variable severity were used to probe immunoblots of electrophoresed e ... | 1991 | 1719692 |
| screening tests for pathogenic corynebacteria. | to provide simple tests that would help in the identification of corynebacteria that produce diphtheria toxin. | 1992 | 1740514 |
| histopathology of the early phase during experimental corynebacterium pseudotuberculosis infection in lambs. | histological responses during experimental corynebacterium pseudotuberculosis infection in lambs were investigated in parotid lymph nodes for ten days following inoculation. lambs were infected by the subcutaneous route into the right eyelid with a virulent strain of c. pseudotuberculosis. multiple microscopic acute abscesses, predominantly infiltrated with polymorphonuclear (pmn) leucocytes, were seen in the right parotid lymph node on the 1st day post-inoculation (pi). this massive pmn infiltr ... | 1991 | 1746152 |
| immunisation against ovine caseous lymphadenitis: comparison of corynebacterium pseudotuberculosis vaccines with and without bacterial cells. | sheep were immunised with corynebacterium pseudotuberculosis vaccines prepared from cell-free toxoid or from toxoid with formalin-killed cells of c pseudotuberculosis added. resistance of sheep to infection was tested 6 months after immunisation by inoculation with caseous lymphadenitis pus. the outcome was assessed 3 months later by slaughter and inspection of the sheep for lesions of caseous lymphadenitis. immunised sheep were adequately protected against infection as shown by a significant re ... | 1991 | 1755781 |
| immunisation against ovine caseous lymphadenitis: efficacy of monocomponent corynebacterium pseudotuberculosis toxoid vaccine and combined clostridial-corynebacterial vaccines. | sheep were immunised with corynebacterium pseudotuberculosis toxoid formulated as a monocomponent vaccine with aluminium adjuvant or in combination with 5 clostridial antigens, and also in the combined form with sodium selenate. immunised and control sheep were experimentally infected 16 days after vaccination and slaughtered and inspected after a further 3 months to determine their resistance to infection. all 3 vaccines afforded an equal and high level of protection; 91% of vaccinated sheep ex ... | 1991 | 1755782 |
| immunisation against ovine caseous lymphadenitis: correlation between corynebacterium pseudotuberculosis toxoid content and protective efficacy in combined clostridial-corynebacterial vaccines. | groups of sheep were dosed with vaccines containing corynebacterium pseudotuberculosis toxoid combined in varying amounts with 5 clostridial antigens. resistance of the sheep to infection with c pseudotuberculosis was tested at 1, 6 and 12 months after vaccination by infection with pus from ovine lymph glands actively infected with c pseudotuberculosis. the outcome was assessed 3 months after challenge by slaughter and inspection of the sheep for caseous lymphadenitis lesions. protection was dem ... | 1991 | 1755783 |
| a field trial to evaluate a whole cell vaccine for the prevention of caseous lymphadenitis in sheep and goat flocks. | a field trial to evaluate a whole cell vaccine for the prevention of caseous lymphadenitis (cla) in sheep and goats was performed in one goat herd and one sheep flock over a period of three years. in goats, there was a nonstatistically significant trend for fewer cases of cla in the vaccinated animals compared to the controls. in sheep, from six months to 36 months postinitial vaccination, the proportion of vaccinated sheep that developed cla was significantly less (p less than 0.05) than in the ... | 1991 | 1790493 |
| selective medium containing fosfomycin, nalidixic acid, and culture supernatant of rhodococcus equi for isolation of corynebacterium pseudotuberculosis. | fnr medium containing fosfomycin, nalidixic acid, bovine blood and culture supernatant of rhodococcus equi was prepared by the present authors, and the medium did not inhibit growth of corynebacterium pseudotuberculosis but completely hampered the growth of bacillus subtilis, staphylococcus aureus, and escherichia coli. the culture supernatant of r. equi facilitated detection of suspected colonies of c. pseudotuberculosis due to synergistic hemolysis. rate of isolation of the organisms (from the ... | 1991 | 1796687 |
| antimicrobial susceptibility of corynebacterium pseudotuberculosis isolated from lesions of caseous lymphadenitis in sheep in hokkaido, japan. | 1991 | 1830812 | |
| lymphocyte subpopulations in pyogranulomas of caseous lymphadenitis. | pyogranulomas of ovine caseous lymphadenitis (cla) are encapsulated lesions resulting from infections with corynebacterium pseudotuberculosis, a bacterial pathogen able to grow within macrophages. immunohistology of cla lesions showed a band of lymphocytes lining the inside of the collagen capsule in intimate contact with necrotic tissue, the intracapsular lymphocytes being organized into three layers. the innermost layer, immediately adjacent to the central necrotic tissue consisted of a narrow ... | 1991 | 1833096 |
| transformation of corynebacterium pseudotuberculosis by electroporation. | corynebacterium pseudotuberculosis was transformed by electroporation, using png2, an erythromycin-resistance plasmid from c diphtheriae. corynebacterium pseudotuberculosis cultivated in brain-heart infusion broth was washed 3 times with water, and resuspended to a final concentration of about 5 x 10(13) colony-forming units/ml. an electroporator constructed in our laboratory incorporated an electrode with 0.8-mm interelectrode gap, using disposable spectrophotometer cuvettes as containers for e ... | 1991 | 1928906 |
| antigen specificity and activity of ovine antibodies induced by immunization with corynebacterium pseudotuberculosis culture filtrate. | sheep were immunized three times with a vaccine composed of filtrate from a 36 h culture of corynebacterium pseudotuberculosis and a block polymer adjuvant. immunization resulted in the development of exotoxin-neutralizing antibody. this corresponded to the recognition of a 31.6 kda protein on sequential immunoblots of ammonium sulfate-precipitated filtrate. in addition sera from vaccinated sheep recognized at least eight bacterial cellular antigens on immunoblots of ether-extracted c. pseudotub ... | 1991 | 1949570 |
| epizootic of external and internal abscesses in a large goat herd over a 16-year period. | from 1973 through 1988, 518 external and internal abscesses were diagnosed in a large commercial goat herd. of the 518 abscesses, 238 were primary and 280 were secondary abscesses in the same or other anatomic locations. during this period, the herd varied in size from 94 goats in 1973 to 431 goats in 1988. abscesses in the jaw area were detected in 141 goats, 4 of which developed osteomyelitis of the mandible. sternal abscesses were detected in 72 goats, with 2 also having osteomyelitis of the ... | 1991 | 2004986 |
| experimental corynebacterium pseudotuberculosis infection in lambs: kinetics of bacterial dissemination and inflammation. | infection and pyogranulomas induced by corynebacterium pseudotuberculosis were experimentally reproduced in lambs. in two separate experiments, bacterial multiplication and dissemination were studied in 30 male lambs inoculated subcutaneously into the right ear with 1.1 or 1.5 x 10(8) viable c. pseudotuberculosis strain 19r. infected lambs were necropsied at various times until the 28th day following inoculation. after a transient hyperthermia and a strong local inflammatory reaction, an abscess ... | 1991 | 2031305 |
| corynebacterium pseudotuberculosis: in vitro susceptibility to 39 antimicrobial agents. | the minimal inhibitory concentrations of 39 antimicrobial agents for 54 isolates of corynebacterium pseudotuberculosis in vitro have been determined. the most active agents were penicillins, macrolides, tetracyclines, cephalosporins, lincomycin, chloramphenicol, and rifampicin. most isolates were resistant to aminoglycosides, nitrofurans, polymyxins, nalidixic acid, and cycloheximide. | 1991 | 2063546 |
| the effect of antibody to caseous lymphadenitis in ewes on the efficacy of vaccination in lambs. | the effect of maternal antibody to the toxin of corynebacterium pseudotuberculosis, produced by caseous lymphadenitis (cla) in ewes or by vaccinating ewes before lambing, on the efficacy of vaccination against cla in their lambs was examined. lambs were allocated to treatments according to either the vaccination history of their dam or level of cla toxin antibody of their dam. they were vaccinated twice using 2 different inoculation regimes and then artificially infected with cla organisms. the ... | 1991 | 2069543 |
| recruitment of 99m-technetium- or 111-indium-labelled polymorphonuclear leucocytes in experimentally induced pyogranulomas in lambs. | the recruitment of polymorphonuclear leucocytes (pmns) during the development of experimental pyogranulomas induced by corynebacterium pseudotuberculosis was followed in nine male lambs by scintigraphic examination. autologous blood pmns were labelled with 99m-technetium or 111-indium and were re-injected intravenously into infected lambs. the functional properties of the labelled cells were monitored 1) in vitro by measuring their phagocytic and bactericidal activity against c. pseudotuberculos ... | 1990 | 2168466 |
| differential antibody responses to corynebacterium pseudotuberculosis in sheep with naturally acquired caseous lymphadenitis. | enzyme-linked immunosorbent assays (elisa) with corynebacterium pseudotuberculosis cell wall and bacteria-free supernatant with exotoxin preparations as antigens, and hemolysis inhibition tests were used to detect antibodies in the sera of adult range sheep with naturally acquired caseous lymphadenitis (cl). the extent and severity of lesions were quantitated on the basis of a lesion score, derived from an examination of the carcass (peripheral lymphoid tissue) and viscera (including internal ly ... | 1990 | 2189849 |
| protein g-based enzyme-linked immunosorbent assay for anti-mpb70 antibodies in bovine tuberculosis. | mpb70 is a highly species specific protein which is secreted from mycobacterium bovis during culture. to investigate whether antibodies against mpb70 can be used as an indicator of infection with m. bovis, an enzyme-linked immunosorbent assay was developed, based on the use of biotinylated protein g, to provide a common indicator for antibody formation in different species. during experimental infection with m. bovis in cattle, a characteristic pattern of anti-mpb70 antibody production was obser ... | 1990 | 2191012 |
| mass spectrometric analysis of o-peracetylated derivatives of 1-monomycoloylglycerol isolated from corynebacterium pseudotuberculosis, rhodococcus rhodochrous and r. lentifragmentus. | 1-monomycoloylglycerols from corynebacterium pseudotuberculosis, rhodococcus rhodochrous and r. lentifragmentus were reacted with acetic acid ahydride in the presence of pyridine. on infrared spectra the reaction products showed a sharp characteristic absorption of the acetyl ester group at 1235 cm-1; the hydroxyl group absorption (3400 cm-1) was absent. o-peracetylated monomycoloylglycerols were analyzed by mass spectrometry under electron impact mode. the most common and representative peaks w ... | 1990 | 2225237 |
| corynebacterium pseudotuberculosis in milk of cl affected goats. | 1990 | 2260259 | |
| effect of muramyl dipeptide on immunogenicity of corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs. | colostrum-deprived lambs and cf1 mice were vaccinated with water-in-oil emulsion vaccines containing nonviable whole cells (wc) of corynebacterium pseudotuberculosis with and without muramyl dipeptide (mdp). efficacy of vaccines was determined from the survival of mice and lesions in lambs after iv injection of 10(4) colony-forming units of c pseudotuberculosis. in mice, protection was related to the concentration of wc in the vaccine. at 50, 100, or 150 micrograms of wc, protection was good (78 ... | 1990 | 2301830 |
| in vitro susceptibility of selected veterinary bacterial pathogens to ciprofloxacin, enrofloxacin and norfloxacin. | the minimum inhibitory concentrations (mic) of ciprofloxacin, enrofloxacin, and norfloxacin were tested for approximately ten clinical isolates of each of actinobacillus pleuropneumoniae, actinobacillus suis, actinomyces pyogenes, corynebacterium pseudotuberculosis, erysipelothrix rhusiopathiae, haemophilus parasuis, haemophilus somnus, pasteurella haemolytica, pasteurella multocida, rhodococcus equi, streptococcus equi, streptococcus suis and streptococcus zooepidemicus. ciprofloxacin and enrof ... | 1990 | 2306672 |
| changes in ovine erythrocyte morphology due to sphingomyelin degradation by corynebacterium pseudotuberculosis phospholipase d. | corynebacterium pseudotuberculosis produces a sphingomyelin-specific phospholipase d exotoxin that is a major determinant in the pathogenesis of caseous lymphadenitis. we assessed the changes induced in the morphology and sphingomyelin concentration of ovine erythrocytes after incubation with broth culture filtrates or purified exotoxin. sphingomyelin was hydrolysed nearly in parallel with shape change. morphologically, spherostomatocytes were seen initially, and later these contained numerous v ... | 1990 | 2348780 |
| alterations in the phospholipid composition and morphology of ovine erythrocytes after intravenous inoculation of corynebacterium pseudotuberculosis. | corynebacterium pseudotuberculosis produces a sphingomyelin-specific phospholipase d exotoxin that is a major determinant in the pathogenesis of caseous lymphadenitis. the effect of this exotoxin on erythrocytes was assessed during experimentally induced infection of sheep. blood was drawn at timed intervals, and the phospholipid composition of erythrocytes was determined by use of high-performance liquid chromatographic analysis of membrane extracts. erythrocyte morphology was determined by use ... | 1990 | 2368943 |
| cloning and expression of the phospholipase d gene from corynebacterium pseudotuberculosis in escherichia coli. | a toxic phospholipase d (pld) is putatively involved in pathogenesis of corynebacterium pseudotuberculosis infections. we report here the cloning and expression of the pld gene (pld) in escherichia coli. a cosmid library of dna from c. pseudotuberculosis biovar ovis isolate whetten 1 was constructed and screened for pld-producing recombinants by plating them on lb agar containing sheep erythrocytes and equi factors. one recombinant, designated pcpo1, yielded a gene product which displayed synerg ... | 1990 | 2403529 |
| cloning, nucleotide sequence, and expression in escherichia coli of the phospholipase d gene from corynebacterium pseudotuberculosis. | the phospholipase d (pld) gene from corynebacterium pseudotuberculosis has been cloned, sequenced, and expressed in escherichia coli. analysis of dna sequence data reveals a major open reading frame encoding a 31.4-kilodalton protein, a size consistent with that estimated for the pld protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. comparison of these data with the amino-terminal protein sequence indicates that the mature pld protein is preceded by a 24-residue signal sequen ... | 1990 | 2407718 |
| keratinolytic activity of cutaneous and oral bacteria. | a test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (ph 7.2) with 1 mm dithiothreitol. the degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. several oral and cutaneous bacteria were investigated: bacteroides gingivalis, bacteroides intermedius, treponema dent ... | 1987 | 2434427 |
| seroepidemiological survey of corynebacterium pseudotuberculosis infection in sheep in japan using enzyme-linked immunosorbent assay and immunodiffusion. | sera from 1186 apparently healthy sheep in hokkaido were examined by enzyme-linked immunosorbent assay (elisa) and immunodiffusion (id) for the presence of antibodies against corynebacterium pseudotuberculosis. elisa-positives were 466 (39.3%) while id-positives were 330 (27.8%). spread of caseous lymphadenitis in sheep in hokkaido was thus clarified. although id was less sensitive than elisa in detecting the antibodies against c. pseudotuberculosis it did not give any non-specific reaction. fro ... | 1989 | 2514314 |
| phospholipase c in listeria. | the cooperative and antagonistic effect of extracellular bacterial proteins of streptococcus agalactiae, rhodococcus equi, corynebacterium ovis, and corynebacterium haemolyticum with proteins of listerial strains of various sources, species and serovars of the membrane of sheep erythrocytes was investigated. listeria monocytogenes and listeria ivanovii produce, beside listeriolysin, further proteins. their specific effect on the sheep erythrocyte membrane becomes apparent after the appearance of ... | 1989 | 2561039 |
| diagnosis of corynebacterium pseudotuberculosis infections in sheep, using an enzyme-linked immunosorbent assay. | lambs infected with corynebacterium pseudotuberculosis (ovis) by injecting suspensions of live bacteria into the wool-free area of the axilla developed antibody against cell wall antigens and antitoxin, as measured by the enzyme-linked immunosorbent assay. the toxin was a better antigen for measuring infection than was the cell wall antigen. the enzyme-linked immunosorbent assay appeared to be as sensitive as the antihemolysin inhibition test for detecting antitoxin and was easier to perform. | 1985 | 2578756 |
| separation of homologues of methyl ester and 3-o-acetyl methyl ester derivatives of the corynomycolic acid fraction from corynebacterium pseudotuberculosis. | 1989 | 2592501 | |
| corynebacterium pseudotuberculosis infection in camels (camelus dromedarius) in saudi arabia. | 1989 | 2617677 | |
| corynebacterium pseudotuberculosis infection in goats. viii. the effect of vaccination against experimental infection. | the effect of an inactivated vaccine against c. pseudotuberculosis infection was tested on castrated male kids from a herd free from caseous lymphadenitis. the animals were divided into 3 groups with 8 animals in each. group 1 was immunized with crude filtrated c. pseudotuberculosis toxoid and whole killed organisms, while group 2 in addition was given levamisole. the kids were vaccinated twice at an interval of 4 weeks. group 3 consisted of unvaccinated animals. all groups were challenged subcu ... | 1989 | 2629504 |
| corynebacterium pseudotuberculosis infection in goats. ix. the effect of vaccination against natural infection. | a vaccination trial was carried out in 10 infected herds. the trial included 247 female kids, the number of animals in each herd varying from 15 to 38. about half of the animals in each herd were vaccinated twice at 3 to 4 week intervals, the first vaccination being carried out before the age of 4 months. a combination of a crude filtrate of c. pseudotuberculosis toxoid with whole organisms, was used. overall, the prevalence of animals with superficial swellings was higher in the unvaccinated th ... | 1989 | 2629505 |
| sheep major histocompatibility (ola) complex: apparent involvement in a flock endemic infection by corynebacterium pseudotuberculosis. | in a préalpe flock with endemic infection by corynebacterium pseudotuberculosis, some sires seem to have transmitted some resistance traits not yet genetically defined. in sire progenies including offspring with and without abscess, abscesses occurred independently of the transmitted sire's ola haplotype. however, in all considered offspring, some ola antigens were positively or negatively associated with the delayed occurrence of abscess. the three antigens ola-a4, a10, b6 were positively assoc ... | 1989 | 2631799 |
| serological investigation of corynebacterium pseudotuberculosis infection in sheep--correlation between the hemolysis inhibition test and the elisa test. | 1989 | 2675582 | |
| the use of a microagglutination assay for the detection of antibodies to corynebacterium pseudotuberculosis in naturally infected sheep and goat flocks. | two goat flocks comprising 326 animals and four sheep flocks comprising 343 animals, all with a previously recognized problem of abscesses due to corynebacterium pseudotuberculosis, were examined for the presence of abscesses and antibody titers to c. pseudotuberculosis as detected by direct microagglutination assay. in sheep there was a strong positive relationship between age and titer (p less than 0.0001). however, the relationship in goats between age and titer could not be determined due to ... | 1989 | 2766152 |
| a seroepidemiological investigation of corynebacterium pseudotuberculosis infection in sheep flocks in southern norway. | 1989 | 2782227 | |
| purification of the phospholipase d of corynebacterium pseudotuberculosis by recycling isoelectric focusing. | recycling isoelectric focusing was investigated as a method for purification of phospholipase d (pld) from cultures of corynebacterium pseudotuberculosis. supernatant fluids from cultures of equine isolate 155 in brain-heart infusion broth were dialyzed against distilled water, concentrated by lyophilization, and fractionated by preparative isoelectric focusing in free solution in a ph 3 to 13 gradient with 6m urea. protein concentration, ph, and pld activity of the 10 resulting fractions were d ... | 1989 | 2782713 |
| comparison of the exotoxins of four strains of corynebacterium pseudotuberculosis. | the activity of exotoxins produced by four strains of corynebacterium pseudotuberculosis were compared by their ability to kill white mice, their haemolytic activity, staphylococcal haemolysin-inhibiting effect and activity in an enzyme-linked immunosorbent assay (elisa). exotoxins with a haemolytic titre of 1 in 256 or more killed all mice and had the most inhibitory effect (1 in 64 or more) on staphylococcal haemolysin. the haemolytic test and staphylococcal haemolysin inhibitory test of exoto ... | 1989 | 2799075 |
| biochemical and genetic characterization of corynebacterium pseudotuberculosis. | isolates (n = 94) of corynebacterium pseudotuberculosis were obtained from sheep, goats, horses, and cattle from various parts of the world. the isolates were characterized biochemically and by restriction endonuclease analysis of dna. we found near homogeneity in the ability of isolates to ferment carbohydrates and to produce urease. all isolates produced phospholipase d and catalase. the ability of isolates from horses to reduce nitrate, the inability of isolates from sheep and goats to do so, ... | 1988 | 2831763 |
| a retrospective study of hepatic abscesses in goats: pathological and microbiological findings. | post-mortem examination of 658 goats showed that 17 (2.5%) exhibited hepatic abscesses. the following bacteria were isolated: corynebacterium pseudotuberculosis (58.9%), escherichia coli (11.8%), corynebacterium sp. (11.8%), pasteurella haemolytica (5.9%), proteus sp. (5.9%) and staphylococcus aureus (5.9%). hepatic abscesses occurred more frequently in adult animals. eleven of the 17 goats (64.7%) were older than 12 months of age. four goats (23.5%) were 12 months of age and only two (11.8%) we ... | 1989 | 2920279 |
| corynebacterium pseudotuberculosis: biochemical properties, production of toxin and virulence of ovine and caprine strains. | twenty-two strains of corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis, were isolated from typical abscesses in sheep and goats from flocks in 6 different regions of france and were characterized. these strains were uniform in biochemical characteristics, susceptibility to 8 antimicrobial agents, and virulence for of1 mice. all these strains produced an exotoxin, identified by inhibition of staphylococcal beta-hemolysin. moreover, this study showed that strain 19r ... | 1989 | 2930134 |
| immunophenotype of pulmonary cellular infiltrates in sheep with visceral caseous lymphadenitis. | pulmonary lesions associated with corynebacterium ovis were analyzed with an indirect immunoperoxidase staining technique using monoclonal antibodies. the predominant cells in abscess walls and surrounding lung parenchyma were large macrophages which expressed major histocompatibility complex (mhc) class ii molecules on their surfaces. t lymphocytes were prominent in the same sites in the naturally occurring lesions, and sbu-t4-positive ("helper/inducer") cells were the major subset of lymphocyt ... | 1988 | 2976546 |
| the effect of selenium supplementation on immunity, and the establishment of an experimental haemonchus contortus infection, in weaner merino sheep fed a low selenium diet. | immunity in 12 weaner merino sheep fed a low selenium (se) diet (low se sheep) was compared with that in 10 matching sheep fed the same diet but each given an intraruminal se pellet (high se sheep), while the sheep were housed in individual, sheltered pens. all sheep were challenged with killed brucella abortus cells (days 0 and 28), rabbit red blood cells (days 0, 7 and 28) and corynebacterium pseudotuberculosis toxoid (days 0 and 28), and serum antibody titres were measured weekly for 8 weeks ... | 1988 | 3138968 |
| [caseous lymphadenitis in goats in gelderland: prospects for its control]. | an elisa was developed for the detection of antibodies to the exotoxin of corynebacterium pseudotuberculosis. experience with the test for control of caseous lymphadenitis in goats in the province of gelderland is discussed. | 1988 | 3212762 |
| influence of selenium on antibody production in sheep. | three experiments were carried out, using sheep fed a marginally low selenium diet, to study the effect of selenium supplementation on the antibody response to tetanus toxoid and on the serum igg concentration. six groups of three six-month-old lambs were fed a basal diet containing 0.13 mg se kg-1 supplemented with either 0.1, 0.5 or 1.0 mg se kg-1, as sodium selenite or as selenomethionine. these animals generally showed enhanced antibody response to tetanus toxoid, parainfluenza-3 virus and c ... | 1988 | 3222551 |
| in vitro susceptibility of bacteria to a ticarcillin-clavulanic acid combination. | in vitro testing of bacterial susceptibility to a combination of ticarcillin and clavulanic acid was done, using 406 aerobic gram-positive and gram-negative isolates (considered to be pathogens) cultured from equine and small animal specimens. a microdilution broth technique of susceptibility testing was performed, using trays with wells containing a range of doubling concentrations of dehydrated ticarcillin (range, 0.50 to 128 micrograms/ml) with fixed concentration of clavulanic acid (4 microg ... | 1988 | 3239838 |
| corynebacterium pseudotuberculosis infection in goats. vi. clinical and pathological changes after intravenous inoculation of the organism. | 1988 | 3256227 | |
| corynebacterium pseudotuberculosis infection in goats. vii. clinical, pathological, serological and hematological changes after subcutaneous inoculation of the organism. | 1988 | 3256228 | |
| pharmacokinetics and body fluid and endometrial concentrations of trimethoprim-sulfamethoxazole in mares. | six healthy adult mares were each given a single iv injection of trimethoprim (tmp)-sulfamethoxazole (smz) at a dosage of 2.5 mg of tmp/kg of body weight and 12.5 mg of smz/kg. serum concentrations of each drug were measured serially over a 24-hour period. for tmp, the mean overall elimination rate constant (k) was 0.43/hr and the elimination half-life (t1/2) was 1.9 hours. the apparent volume of distribution (at steady state) was 1.62 l/kg and tmp clearance was 886 ml/hr/kg. for smz, k was 0.22 ... | 1988 | 3261143 |
| corynebacterium pseudotuberculosis infection in adult ewes by inoculation in the external ear. | caseous lymphadenitis, caused by corynebacterium pseudotuberculosis, was studied in ewes to devise a reliable method of induced infection that would help to understand mechanisms by which abscesses develop in lymph nodes or internal organs, especially the lungs. fifteen adult ewes, from a flock where the occurrence of caseous lymphadenitis was unknown, were inoculated sc in the auricle with various doses of a virulent strain of c pseudotuberculosis. ewes inoculated with at least 10(8) viable cor ... | 1988 | 3377305 |
| the effects of caseous lymphadenitis on wool production and bodyweight in young sheep. | two hundred merino wether hoggets were used to examine the effect of corynebacterium pseudotuberculosis infection (caseous lymphadenitis) on wool production and bodyweight. sheep which were challenged with c. pseudotuberculosis (artificially infected) and not vaccinated against this disease produced 0.20 kg less clean wool than unchallenged controls during the following 12 months. the incidence of sheep with lesions in the group that was vaccinated prior to challenge was 55% lower than in unvacc ... | 1988 | 3390089 |
| corynebacterium folliculitis in a horse. | a 7-year-old thoroughbred was examined for evaluation of mildly pruritic multiple skin lesions that had progressed from nodules to alopecia and crusts. folliculitis caused by corynebacterium pseudotuberculosis was diagnosed by bacterial culture. oral treatment with a trimethoprimsulfadiazine paste resulted in resolution of all lesions, with normal hair regrowth. | 1988 | 3417537 |
| the role of corynebacterium pseudotuberculosis lung lesions in the transmission of this bacterium to other sheep. | five groups of 5 shorn and 5 unshorn caseous lymphadenitis (cla)-free merino wether weaners were each placed in feedlot pens with 6 merino ewes, 2 or more of which had cla lung lesions but no discharging superficial lesions. the sheep were kept together for 5 months. twenty-eight per cent of the shorn weaners and 20% of the unshorn weaners developed antibodies to corynebacterium pseudotuberculosis. at slaughter, 8% of the shorn weaners and 12% of the unshorn weaners had cla lesions in either lun ... | 1987 | 3426463 |
| evaluation of an enzyme-linked immunosorbent assay for the detection of corynebacterium pseudotuberculosis infection in sheep. | two enzyme-linked immunosorbent assays to measure antibody to the cell wall antigen (c-elisa) and toxin antigen (t-elisa) of c. pseudotuberculosis were evaluated on serum from 6 separate groups of sheep. for sheep naturally infected with c. pseudotuberculosis the sensitivity and specificity of the c-elisa was 76% and 73% respectively and for the t-elisa 67% and 77% respectively. for sheep slaughtered one year after artificial infection the sensitivity of both tests was greater than or equal to 8 ... | 1987 | 3426464 |
| corynebacterium ovis (pseudo-tuberculosis) lymphadenitis in a sheep farmer: a new occupational disease in new zealand. | corynebacterium ovis lymphadenitis is a common disease in high country sheep in new zealand and has previously been reported in humans overseas. the first human case is reported in new zealand with a sheep confirmed as the infection source. | 1986 | 3463898 |
| factors affecting the yield of viable cells of corynebacterium pseudotuberculosis in a liquid medium. | growth of corynebacterium pseudotuberculosis in an undefined liquid medium was studied with the aim of obtaining uniform, single-cell suspensions of the organism suitable for experimental infection. adequate yields of viable cells were shown to be dependent on a fermentable substrate and a buffering system in a medium containing tween 80. the development of nutrient tween 80 broth for the culture of c. pseudotuberculosis is considered to be a significant advance particularly for studies involvin ... | 1986 | 3518223 |
| synergistic hemolysis-inhibition titers associated with caseous lymphadenitis in a slaughterhouse survey of goats and sheep in northeastern brazil. | a survey of caseous lymphadenitis was conducted at a goat and sheep slaughterhouse in northeastern brazil one hundred and fifty-eight goats and 43 sheep were examined for the presence of abscesses, with bacterial culturing of purulent material to define the etiological agent. blood was collected simultaneously for determination of serological titer via the synergistic hemolysis-inhibition test which measures antibodies to an exotoxin of corynebacterium pseudotuberculosis. thirteen and nine-tenth ... | 1987 | 3567751 |
| corynebacterium pseudotuberculosis infection in sheep and the complement fixation test for paratuberculosis. | 1987 | 3576925 |