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interactions between bifidobacterium bifidum n4 and escherichia coli k-12 in their mixed cultures.the interactions between bifidobacterium bifidum n4 (b. bifidum) and escherichia coli k-12 (e. coli) were investigated in their mixed cultures. under conditions in which both bacteria grew well in their pure culture, b. bifidum inhibited the growth of e. coli even when the latter was inoculated at 10(4)-fold and preincubated for 41 hr. the inhibition in the mixed cultures appeared when the ph values were reduced below 4.6. when the lowering of ph was prevented by the addition of naoh, no inhibit ...197837291
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. ix. metabolism and release of cellular lipids in the presence of antibiotics.inhibiton of cell wall synthesis caused simultaneously an increase in cellular phospho-and glycolipids and a marked release of these compounds to the medium. the composition of the cellular and the released glyco-and phospholipids was almost the same. antibiotics, which inhibit cell wall synthesis, did not influence glycolipid composition, but increased the relative and absolute amounts of disphosphatidylglycerol and its lysoderivatives. incorporation and chase experiments demonstrated a conside ...197664257
lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes.the respiratory quinones of 73 strains of gram-positive bacteria including spore-forming rods, lactic-acid bacteria and actinomyctes were examined. menaquinones with seven isoprenoid units (mk-7) were the main quinone type found in representatives of the genus bacillus and in sporolactobacillus inulinus. however, a strain of b. thuringiensis produced mk-8 in addition to mk-7, and strains of b. lentus and b. pantothenticus appeared to produce mk-9 and mk-8, respectively, with no mk-7. in the clos ...1979119033
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. viii. composition and metabolism of phospholipids at different stages and conditions of growth.1. the phospholipid content and composition of bifidobacterium bifidum var. pennsylvanicus is markedly influenced by the growth phase, the ph and the presence of human milk in the culture medium. 2. the lipid-phosphorus content of the cells increases during the first period of active growth, but decreases later. the lipid-phosphorus content of the cells in the stationary phase is at constant ph 5.5 about 45 percent of that at constant ph 6.8 and final ph 5.2. this difference is caused by a gener ...1975237545
[antagonistic interrelationships of bifidobacterium bifidum i proteus vulgaris in vitro in the digestive tract of gnotobiotic chicks].the antagonistic relations between bacterium bifidum, strain i/850 phi, and proteus vulgaris, strain f-30, were studied. these organisms, when introduced together in equal doses into the digestive tract of gnotobiotic chickens in a single administration, were shown to create certain ecological correlations in various organs with the prevalence of bifidobacteria which exerted no negative influence on proteus vulgaris. the additional daily administration of bifidobacteria for 3 days running in dos ...1979382712
purification and properties of udp-glucose (udp-galactose) pyrophosphorylase from bifidobacterium bifidum.an enzyme having both udp-glucose (udp-glc) and udp-galactose (udp-gal) pyrophosphorylase activities was purified to homogeneity from bifidobacterium bifidum. the molecular weight of the enzyme was about 200,000 and it appeared to be composed of four identical subunits. the purified enzyme showed almost the same reactivity towards udp-glc and udp-gal, and showed about 10% of this activity towards udp-xylose at 8 mm. the enzyme required magnesium ions for maximum activity. the apparent equilibriu ...1979500588
fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon.a total of 154 strains from 22 species of bifidobacterium, peptostreptococcus, lactobacillus, ruminococcus, coprococcus, eubacterium, and fusobacterium, which are present in high concentrations in the human colon, were surveyed for their ability to ferment 21 different complex carbohydrates. plant polysaccharides, including amylose, amylopectin, pectin, polygalacturonate, xylan, laminarin, guar gum, locust bean gum, gum ghatti, gum arabic, and gum tragacanth, were fermented by some strains from ...1977563214
effects of growth conditions on the lipid composition of bifidobacterium bifidum subsp. pennsylvanicum.lipid-phosphorus and lipid-galactose content and phospholipid and fatty acid composition of bifidobacterium bifidum subsp. pennsylvanicum were examined under a wide variety of growth conditions. cells from 29-c cultures contained less lipid-phosphorus than did cells from 37-c cultures, but their lipid-galactose content and phospholipid composition did not differ. at both temperatures, the growth phase influenced the lipid composition similarly. phosphate, mg2+ and k+ concentrations in the medium ...1977596859
effects of growth conditions on the ion composition of bifidobacterium bifidum subsp. pennsylvanicum.the cation content of bifidobacterium bifidum subsp. pennsylvanicum was markedly influenced by the washing procedure of the cells, by the growth phase and the temperature, and by the composition of the culture medium. optimal retention of cations was achieved by washing with 0.25 m mgcl2 at 20c. the intracellular na+ concentration rose during growth in normal medium to a constant value in the stationary phase, the k+ concentration rose in the exponential phase, but fell in the stationary phase. ...1977596860
[fine structure of bifidobacterium bifidum].cultures of bifidobacterium bifidum 1 were grown in liquid and semisolid nutrient media for 14 hours to 6 days. their preparations were then studied by electron microscopy on ultra-thin sections after a routine chemical fixation or on replicas after rapid freezing-fracturing and etching. this procedure made it possible to obtain new data about the structural organization of cells in the course of growth of cultures which were characterized by desynchronization of growth and division processes. t ...1978713883
presence of a single enzyme catalyzing the pyrophosphorolysis of udp-glucose and udp-galactose in bifidobacterium bifidum.the enzyme preparation catalyzing the pyrophosphorolyses of udp-glucose and udpgalactose almost at the same rate was purified about 900-fold from bifidobacterium bifidum grown on glucose medium. the two activities were always associated with each other, and their activity ratio was always constant throughout the purification steps. the final preparations was revealed homogeneous by polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. there was no significant ...1978718964
effects of antibiotics on the cation composition of bifidobacterium bifidum subspecies pennsylvanicum.cell wall-inhibitory antibiotics caused a marked reduction of intracellular k+ and a small increase of na+, together resulting in a decline of intracellular cation content. chloramphenicol and tetracycline increased intracellular k+ but actinomycin decreased both na+ and k+ content. the release and uptake of 86rb+ were similarly affected by the antibiotics as the k+ content. the osmotic resistance of the protoplasts isolated from antibiotic-treated cells was apparently increased by inhibitors of ...1978755153
metabolic relationships between phospho(galacto)lipids in bifidobacterium bifidum var. pennsylvanicus. 1976955100
biosynthesis of phosphogalactolipids and diphosphatidylglycerol in a membrane fraction of bifidobacterium bifidum var. pennsylvanicus. 1976974091
[composition of nucleic acids and concentration of methylated bases in the dna of bifidobacterium bifidum var. tissier].nucleotide composition of the sum total dna of b. bifidum, biotype iii, was determined by paper chromatography in combination with ultraviolet spectrophotometry. dna of b. bifidum was referred to the gc-type (gc -- 62.6 mol%). two additional nitrogen bases were present in the dna composition; 5-methylcytosine and 6-methylaminopurine -- 0.45 mol% and 0.20 mol%, respectively. nucleotide composition of the sum total rna was studied with the aid of high-voltage electrophoresis in combination with ul ...1976983588
effects of antibiotics on metabolism of peptidoglycan, protein, and lipids in bifidobacterium bifidum subsp. pennsylvanicus.the formation of cell envelope components of bifidobacterium bifidum subsp. pennsylvanicus was studied by measuring the incorporation of [(3)h]glycine, (14)c-labeled fatty acids, and n-benzoyl-[(14)c]glucosamine into the membrane protein, membrane lipids, and cell wall peptidoglycan, respectively. inhibition of peptidoglycan synthesis by antibiotics (penicillin g, vancomycin, d-cycloserine, and bacitracin) and by the omission of glucosamine-containing growth factors caused a marked decrease in g ...19761008539
[the incidence of bacterium bifidum excretion in adults with different gastrointestinal diseases]. 19751214655
high-affinity oxygen uptake by bifidobacterium bifidum.oxygen uptake by washed cell suspensions of bifidobacterium bifidum dsm 20082 was studied by using spectrophotometric measurements of the degree of oxygenation of added myoglobin as a measure of the concentration of dissolved o2. the absorbance changes during consumption of o2 in a closed reaction vessel were analysed by computer to obtain estimates of the changes in dissolved o2 concentration with time. these were then used to calculate the rate of o2 uptake as a function of the dissolved o2 co ...19921285646
phylogenetic evidence for the transfer of eubacterium suis to the genus actinomyces as actinomyces suis comb. nov.the 16s rrna primary structures of eubacterium suis dsm 20639t (t = type strain) and bifidobacterium bifidum dsm 20456t were determined by sequencing in vitro amplified rdna. sequence comparisons indicated that b. bifidum is moderately related to representatives of the genera actinomyces and mobiluncus. the closest relative of e. suis is actinomyces pyogenes. e. suis and a. pyogenes are more closely related phylogenetically to one another than to the other actinomyces species that have been inve ...19921371060
[the effect of phospholipids on the adhesive properties of bacteria].the present work shows that choline-containing phospholipids (lysophosphatidylcholine and lyso-1-alkyl-sn-glycerophosphocholine) inhibit the adhesion of some strains: bacterium bifidum 1, b. adolescentis mc-42, b. longum b. 379m, staphylococcus aureus p 209 and klebsiella pneumoniae 52. phosphatidylcholine produces no effect on the adhesiveness of these strains, while platelet activation factor stimulates adhesiveness only in strain s. aureus 209. the stimulating or inhibiting action of phosphol ...19921455967
antibacterial spectrum of lactoferricin b, a potent bactericidal peptide derived from the n-terminal region of bovine lactoferrin.a physiologically diverse range of gram-positive and gram-negative bacteria was found to be susceptible to inhibition and inactivation by lactoferricin b, a peptide produced by gastric pepsin digestion of bovine lactoferrin. the list of susceptible organisms includes escherichia coli, salmonella enteritidis, klebsiella pneumoniae, proteus vulgaris, yersinia enterocolitica, pseudomonas aeruginosa, campylobacter jejuni, staphylococcus aureus, streptococcus mutans, corynebacterium diphtheriae, list ...19921490908
survival of lactobacillus acidophilus and bifidobacterium bifidum in ice cream for use as a probiotic food.probiotic ice cream was made by fermenting a standard ice cream mix with lactobacillus acidophilus and bifidobacterium bifidum cultures and then freezing the mix in a batch freezer. survival of the l. acidophilus and b. bifidum, as well as beta-galactosidase activity, was monitored during 17 wk of frozen storage at -29 degrees c. after freezing of the fermented mix, bacterial counts were 1.5 x 10(8) cfu/ml for l. acidophilus and 2.5 x 10(8) cfu/ml for b. bifidum. seventeen weeks after freezing, ...19921500547
[the effect of lactobacillus acidophilus and bifidobacterium bifidum on the intestinal ecosystem of the elderly patient].the authors have tested the therapeutic efficacy of a multibacterial combination consisting of lactobacillus acidophilus (10(9)) and bifidobacterium bifidum (10(9)) in elderly patients with bowel disorders. bacteriological and histopathologic investigation showed this combination to yield excellent biologic results with restoration of duodenal bacterial flora and subsidence of clinical symptoms. the function of the muciparous glands was restored and the duodenal mucosa was normalized.19921526095
effect of bifidobacterium bifidum and lactobacillus acidophilus on gut mucosa and peripheral blood b lymphocytes.in 15 elderly individuals lyophilized bifidobacterium bifidum (bb) and lactobacillus acidophilus (la) (infloran) were administered in capsules (two capsules 4 times per day) for 28 days, while in 10 elderly controls placebo were given the same posology and for an equal period of time. the effects of this treatment on the immune system both at the periphery or the intestinal level were investigated. results show that bb and la significantly reduced the colonic inflammatory infiltration, without a ...19921597660
partial 16s rrna primary structure of five actinomyces species: phylogenetic implications and development of an actinomyces israelii-specific oligonucleotide probe.the intra- and intergeneric relationships of the genus actinomyces were determined by comparing long 16s rrna sequences, generated by reverse transcriptase. all species formed a phylogenetically coherent cluster in which actinomyces bovis, a. viscosus, a. naeslundii, a. odontolyticus and a. israelii constituted genetically well defined species. a. israelii dsm 43322 (serotype 2) was not closely related to three other strains of this species (serotype 1) and, as judged from phylogenetic distances ...19901693659
[the range of antigenic specificity of bifidobacterium peptidoglycan].the antigenic relationships of bifidobacterium bifidum 1 peptidoglycans with different strains of this species (lva-3, 791, go-4), bifidobacteria of other species (b. adolescentis go-13, b. breve 79-38, b. lactentis 79-41, b. longum go-3) and bacteria of remote taxonomic groups (streptococcus faecalis 6-3. staphylococcus aureus com 885, s. epidermidis com 2124. lactobacillus plantarum 1, escherichia coli m-17) were studied on the basis of a highly sensitive test system permitting the registratio ...19911716036
effect of lactobacillus acidophilus and bifidobacterium bifidum on rectal cell kinetics and fecal ph. 19911742509
[the characteristics of staphylococcus aureus peptidoglycan in the spectrum of the reactivity of human lymphocytes to bacterial peptidoglycans].the sensitivity of lymphocytes of healthy persons to s. aureus peptidoglycan as compared with that to the polyclonal stimulator zymosan c3b and peptidoglycans of other bacteria (streptococcus faecalis, escherichia coli, bacterium bifidum) was analyzed with a test system permitting the determination of specific reactivity to peptidoglycans. the analysis showed that at the peak of luminol-dependent chemiluminescence (25-30 minutes) individual reactivity to s. aureus peptidoglycan varied within wid ...19911759526
enhancing effects of bile salts on the degradation of glycosphingolipids by glycosidases from bacteria of the human fecal flora.different concentrations of ionic and non-ionic detergents were examined for optimization of the in vitro degradations of intestinal glycosphingolipids by alpha- and beta-glycosidases from human fecal bacteria. in 5 mm triton x-100 the enzymes hydrolyzed glycosphingolipids with lactoseries type 1 and 2 chains essentially to lactosylceramide (laccer). in 5 mm sodium di- and trihydroxy bile salts lactosylceramide was degraded to glycosylceramide (glccer) in varying extent by enzymes from all five ...19911854798
establishment of lactobacillus and bifidobacterium species in germfree mice and their influence on some microflora-associated characteristics.germfree mice were inoculated with both lactobacillus acidophilus a10 and bifidobacterium bifidum b11. both strains were established and present in more than 10(8) cells per g of cecum and colon contents. furthermore, l. acidophilus a10 was established in high numbers in stomach and small intestine. contents from different parts of the intestine were investigated with regard to the following microflora-associated characteristics: degradation of mucin, beta-aspartylglycine and tryptic activity, c ...19911872614
[shigella endotoxin protein--its electrophoretic and serological properties].the electrophoretic analysis of lipid a-associated protein (lap), obtained from s. sonnei, in polyacrylamide gel in the presence of sodium dodecyl sulfate and urea has revealed the heterogeneity of the preparation; it has found to contain three main components with molecular weights of 43, 38 and 18 kd and some minor components with molecular weights of 49, 45 35, 30, 29, 27, 5, 21 and 14 kd. the electrophoretic mobility of the main protein components in the isolated preparation of lap coincides ...19911882599
[the autologous intestinal microflora in the postresuscitation period after acute blood loss in rats].in this work the data obtained in the study of intestinal microbiocenosis in rats on the third day of the postresuscitation period after acute blood loss are presented. the quantitative and qualitative shifts of microflora at different biotopes, such as the wall of the small intestine, the parietal microflora of peyer's patches, the contents and wall of the large intestine, have been characterized. some specific features of dysbiotic changes have been revealed in comparison with the shifts of in ...19911882621
effect of lactic acid producing bacteria on the human intestinal microflora during ampicillin treatment.20 healthy volunteers participated in a double blind study concerning the effect of lactic acid producing bacteria on the intestinal microflora during ampicillin treatment. 10 volunteers received 500 mg ampicillin tablets t.i.d. together with capsules containing lactic acid producing bacteria (lactobacillus acidophilus and bifidobacterium bifidum) for 7 days, and the other 10 volunteers were given 500 mg ampicillin tablets together with placebo capsules t.i.d. for 7 days. both groups of voluntee ...19911906634
binding of mutagens by fractions of the cell wall skeleton of lactic acid bacteria on mutagens.the binding effect of cells and cell fractions, cell wall skeleton, cytoplasm, whole cells, and cell wall skeleton treated by lysozyme and alpha-amylase at 37 degrees c for 5 h, on trp-p-1 (3-amino-1,4-dimethyl-[5h]pyrido [4,3-b]indole) and trp-p-2 (3-amino-1-methyl-[5h]-pyrido[4,3-b]indole) were investigated. the cell and cell wall skeleton of streptococcus cremoris z-25 had greater binding activity, but cytoplasm and extract of cell wall skeleton did not bind trp-p-1 and trp-p-2. when the cell ...19911908865
response of bifidobacterium species to growth promoters in human and cow milk.we used an in vitro assay to study and compare the growth-promotional activity of protein and nonprotein components in human milk (hm) and cow milk (cm) samples for infant strains of bifidobacterium species. hm samples varied considerably in growth-promotion activity for bifidobacterium bifidum var pennsylvanicus, bifidobacterium infantis, and bifidobacterium breve. pooled cm samples showed similar but less variable levels of activity when compared with hm samples. separation of milk samples by ...19912014160
in vitro binding of mutagenic pyrolyzates to lactic acid bacterial cells in human gastric juice.the binding of mutagenic pyrolyzates to freeze-dried cells of streptococcus cremoris z-25 was investigated in human gastric juice and compared with the ability of these pyrolyzates to bind in distilled water (ph 6.30). the ph of donated gastric juice ranged from 1.2 to 7.8. binding that occurred in gastric juice was ph dependent, and binding was affected by nacl, cacl2, and mgcl2. les sof trp-p-2 was bound in gastric juice at low ph (1.2, 1.48, and 2.1) than at ph 4 to 8. the strain bound simult ...19912071710
occurrence of bifidobacterium in the feces of newborns delivered by cesarean section.in cesarean section newborns, colonization by bifidobacterium occurs generally within 4 days of life. breast-fed infants, independent of the delivery procedure, harbor a gastrointestinal flora characterized by a predominance of bifidobacterium. breast-fed [corrected] newborns delivered by cesarean section present a predominance of bifidobacterium bifidum and bifidobacterium infantis in their stools. investigation of variation in the incidence of bifidobacterium among cesarean section newborns sh ...19902076442
effect of chronic ingestion of a fermented dairy product containing lactobacillus acidophilus and bifidobacterium bifidum on metabolic activities of the colonic flora in humans.nine healthy volunteers were studied before, during, and after ingesting a fermented dairy product containing lactobacillus acidophilus, bifidobacterium bifidum, and mesophilic cultures (streptococcus lactis and s cremoris) for 3 wk. hydrogen and methane productions and fecal beta-galactosidase and beta-glucosidase activities were measured as indicators of fermentation capacity of the colonic flora. fecal concentrations of nitroreductase, azoreductase, and beta-glucuronidase, which may be implic ...19902119557
primary structure of ten galactosides formed by transglycosylation during lactose hydrolysis by bifidobacterium bifidum.oligosaccharides formed by a transgalactosylation reaction during lactose hydrolysis with bifidobacterium bifidum were separated into eight fractions by gel-permeation chromatography and their structures studies determined by trimethylsilylation analysis, methylation analysis, f.a.b.-m.s., g.l.c.-m.s. and enzymic hydrolysis as beta-d-galp-(1----3)-d-glc, beta-d-galp-(1----6)-d-glc, beta-d-galp-(1----6)-d-gal, beta-d-galp-(1----3)-beta-d-galp-(1----4)-d-glc, beta-d-galp-(1----6)[beta-d-galp-(1--- ...19902119886
[competitive activity of a bacterial preparation on colonization and pathogenicity of c. pylori. a clinical study].the authors studied the ability of a live polymicrobial formulation containing lactobacillus acidophilus 10(9) and bifidobacterium bifidum 10(9) to act as an "ecological" therapy for gastritis and duodenitis. the study was carried out in volunteers suffering from these disorders which nowadays are attributed to c. pylori. the results obtained confirm previous findings in experimental animals. the polymicrobial formulation was found to be able to compete effectively with the microorganism respons ...19902147608
structure of macroamphiphiles from several bifidobacterium strains.lipoteichoic acid-like substances, macroamphiphiles, were isolated from cell homogenates of bifidobacterium bifidum yit 4007 and yit 4013, bifidobacterium breve yit 4010 and yit 4014, and bifidobacterium longum yit 4021 by phenol extraction followed by nuclease digestion, gel chromatography, ion-exchange chromatography, and hydrophobic interaction chromatography. the macroamphiphile preparations from these five strains contained d-glucose, d-galactose, glycerol, phosphorus, l-alanine, and fatty ...19902298702
growth promotion of bifidobacterium species by whey and casein fractions from human and bovine milk.an in vitro assay was used to study the growth-promotional activity of human milk (hm), cow's milk (cm), and whey and casein fractions of hm and cm for five strains of bifidobacterium species isolated originally from stools of human infants. whey- and casein-predominant cm-based infant formulas were studied as well. when compared on an equivalent protein basis, the growth promotion activity of hm was greater than that of cm for bifidobacterium bifidum serovar pennsylvanicus and bifidobacterium l ...19902312674
[host-bifidobacterium interactions in the axenic mouse: partial characterization of bifidogenic factors in the intestinal contents].growth factors for bifidobacterium bifidum were detected in faeces of axenic mice strain c3h. most of these factors were found in the nondialyzable fraction obtained after aqueous extraction and dialysis. sds-page and filtration chromatography on sepharose 4b revealed that many glycosylated components harbored a bifidigenic activity. intestinal colinization of mice by b. bifidum involved the utilization and eventually the disappearance of the intestinal bifidigenic factors. there was no change i ...19902357644
uncoupling of growth and acids production in bifidobacterium ssp.kinetics of batch cultivation of four species of bifidobacterium in milk were examined in detail. bifidobacteria could grow well in milk inoculated with cultures prepared in a synthetic medium. cessation of growth occurred, however, in ph-controlled batch cultures, although incomplete utilization of lactose was observed. lactate and acetate accumulation caused limitation on growth of bifidobacteria leading to an uncoupling of biomass and product formation. from 70 to 75% of both final lactate an ...19902384614
immunochemical studies on the lipoteichoic acids of bifidobacterium bifidum subsp. pennsylvanicum.antisera to lipoteichoic acid of bifidobacterium bifidum subsp. pennsylvanicum were obtained by injecting lipoteichoic acid/methylated bsa complexes into rabbits. precipitin tests showed that the glycerol phosphate backbone is primarily responsible for serological specificity while the polysaccharide part of the molecule plays a minor role. whole cells of b. bifidum subsp. pennsylvanicum were capable of absorbing antibodies, indicating the presence of lipoteichoic acid (14% of the total content) ...19852410545
transport of ferrous iron and lactate production in bifidobacterium bifidum var. pennsylvanicus.initial rates of ferrous iron transport into bifidobacterium bifidum var. pennsylvanicus were measured at low and high iron concentrations. the low affinity system (lafius) had an apparent km of 167 microm, the high affinity system (hafius) had a km of 50 microm. iron removal from preloaded bifidobacteria revealed the existence of a labile and an inert iron pool in the bacterial cells. iron uptake by the bifidobacteria was associated with lactate production, though lactate production could conti ...19882484352
[oral bacteriotherapy with bifidobacterium bifidum and lactobacillus acidophilus in cirrhotic patients].thirty-five patients with alcohol cirrhosis were treated for several months (average 4 months) with infloran (6 capsules daily). thirty cirrhotics were treated for the same length of time with lactulose and diuretics. infloran-treated patients showed reduced ammonium levels, improvement of mental status and psychological performance. in addition, patients who required hospitalization had shorter in-patient periods thanks to a more rapid psychological and physical improvement. infloran may be con ...19892534368
modulation of clostridium perfringens intestinal colonization in infants delivered by caesarean section.the colonization by clostridium perfringens was investigated in 19 infants delivered by caesarean section during the two first weeks of life. the pattern of c. perfringens colonization depended upon the feeding. breast feeding led to the repression of c. perfringens, whereas bottle feeding allowed its maintenance. on the contrary, bifidobacterium bifidum growth was favoured by breast feeding. however, in one breast-fed infant, b. bifidum was never isolated and c. perfringens decreased. breast fe ...19892548965
[bacteriotherapy of intestinal dysbacteriosis in patients with acute leukemia].the results have been presented of correcting intestinal microflora in 127 patients with acute leukemia by preparations containing bacillus bifidus and acidophilus: dried and milk bifidum-bacterin, biofructolact, acidophilic milk.19892721896
[action of microbe-antagonists on bacilli and corynebacteria of the skin of the breast in nursing women].five microbes-antagonists are studied for their inhibitory action on 769 strains of bacilli (241) and corynebacteria (528) isolated from the mammary gland skin of 120 nursing women on the 2nd-5th day after labour. escherichia coli m-17 and mycococcus krassilinikovi are established to possess the highest antagonistic properties against the spore-bearing aerobic and coryneform bacteria. bifidobacterium bifidum has exerted a moderate antagonistic action on the culture under study, bacillus subtilis ...19892796786
ferrous iron uptake by bifidobacterium bifidum var. pennsylvanicus: the effect of metals and metabolic inhibitors.ferrous iron uptake studies in bifidobacterium bifidum var. pennsylvanicus were carried out in a well-defined salt solution termed "modified hanks solution" at both high iron concentrations (lafius conditions) and low concentrations (hafius conditions). various divalent metals, mn2+, zn2+, ni2+ and cu2+, inhibited iron uptake under hafius conditions in a non-competitive manner, and in a pseudo-competitive manner under lafius conditions. cr2+ had no effect. co2+ inhibited iron uptake competitivel ...19873038634
evaluation of antinfective activity of an association bifidobacterium/lactobacillus in mouse intestine.the present research aimed at studying the action of an association of bifidobacterium bifidum and lactobacillus acidophilus, in controlling an experimental severe infection by salmonella enteritidis administered in mouse per os. the behaviour of some parameters was undertaken, checking for the ph of intestinal content; the condition of the colonization in the intestinal wall by means of scanning electron microscopy and plates cultures; the presence of antibodies iga in intestinal content; the i ...19873150755
immunoglobulin g to bifidobacterium bifidum in children's sera. 19863551487
'lipoteichoic acid' of bifidobacterium bifidum subspecies pennsylvanicum dsm 20239. a lipoglycan with monoglycerophosphate side chains.the lipid macroamphiphile of bifidobacterium bifidum subsp. pennsylvanicum dsm 20239 was extracted with phenol/water and purified by treatment with nucleases and hydrophobic interaction chromatography. from analytical data, the results of smith degradation, hydrolysis with hf and methylation studies, the following structure is proposed: (formula; see text) where n and m are approximately 7-10 and 8-15, respectively. the monoglycerophosphate residues have the sn-glycero-1-phosphate configuration; ...19873595606
analysis of the lipoteichoic-acid-like macroamphiphile from bifidobacterium bifidum subspecies pennsylvanicum by one- and two-dimensional 1h- and 13c-nmr spectroscopy.the native lipoteichoic-acid-like amphiphile from bifidobacterium bifidum subspecies pennsylvanicum and its basic glycan moiety, obtained by alkaline hydrolysis, were studied by 1h-, 13c-, and 31p-nmr, dept (distorsionless enhancement by polarization transfer), 1h-1h correlation spectroscopy and 13c-1h shift correlation nmr spectroscopy. the results are consistent with the structure elucidated by chemical analysis (fischer, w., preceding paper in this journal): (formula; see text) and establish ...19873595607
mechanisms of ferric and ferrous iron uptake by bifidobacterium bifidum var. pennsylvanicus.iron uptake studies in bifidobacterium bifidum var. pennsylvanicus were carried out using ferric citrate at iron concentrations above 0.01 mm and ph 7, ferrous iron at concentrations less than 0.01 mm at ph 5. two ferric iron transport systems were distinguished: the temperature-insensitive polymer, and the temperature-sensitive monomer uptake. both showed a saturation phenomenon. the transport of ferrous iron at concentrations below 0.01 mm was temperature-dependent, and its affinity for iron w ...19863698473
antibody response to anaerobic coccoid rods in crohn's disease.the igg and igm specific antibodies against a panel of 23 anaerobic gut bacteria were examined in crohn's disease, ulcerative colitis, and healthy controls. four of the organisms, bifidobacterium bifidum, coprococcus comes (me46), coprococcus comes (sp4), and eubacterium limosum gave abnormal antibody titres in crohn's disease compared with those of controls. in crohn's disease specific igg antibodies to three of the organisms were low and the igm antibodies were higher than those of controls. i ...19863782488
interaction of bifidobacterial lipoteichoic acid with human intestinal epithelial cells.binding of the lipoteichoic acids of bifidobacterium bifidum to human colonic epithelial cells appeared to be specific, reversible, and cell concentration and time dependent. a single population of approximately 8.3 x 10(8) binding sites per cell was detected, with a dissociation constant of 125 microm. ester-linked fatty acids are essential for binding.19853965407
modulation of cytotoxin production by clostridium difficile in the intestinal tracts of gnotobiotic mice inoculated with various human intestinal bacteria.gnotobiotic mice died 2 days after inoculation of a cytotoxigenic clostridium difficile strain. protection occurred when mice were previously inoculated with a strain of escherichia coli or bifidobacterium bifidum. intestinal cytotoxin production was highly reduced in the surviving mice, whereas the c. difficile population level did not decrease to a great extent.19853977313
phosphatidylglycerol as biosynthetic precursor for the poly(glycerol phosphate) backbone of bifidobacterial lipoteichoic acid.phosphatidylglycerol functions as donor of the sn-glycerol 1-phosphate units in the synthesis in vitro of the 1,2-phosphodiester-linked glycerol phosphate backbone of the lipoteichoic acids of bifidobacterium bifidum subsp. pennsylvanicum. the incorporation was catalysed by a membrane-bound enzyme system. after addition of chloroform/methanol the product formed coprecipitated with protein. the material was phenol-extractable and was co-eluted with purified lipoteichoic acid on sepharose 6b. the ...19854026803
cell surface hydrophobicity of bifidobacterium bifidum subsp. pennsylvanicum.the possible role of lipoteichoic acid with respect to cell surface properties of bifidobacterium bifidum subsp. pennsylvanicum was studied. standard suspensions of bacteria were mixed with octane or xylene. b. bifidum subsp. pennsylvanicum was shown to possess a strongly hydrophobic cell surface. hydrophobicity of the bacteria could be reduced by treatment with trypsin, pepsin (at ph 4.5), hcl and penicillin. the latter treatment resulted in an increased excretion of lipoteichoic acid. albumin ...19854091536
[on a method of cultivation of bacterium bifidum]. 19684185782
[on the metabolism of bacterium bifidum (lactobacillus bifidus). xi. vitamin requirement in tomarelli's solution]. 19674231075
catabolism of glucose and derivatives of 2-deoxy-2-amino-glucose in bifidobacterium bifidum var. pennsylvanicus. 19694236541
the structure of the cell wall peptidoglycan of bifidobacterium bifidum var. pennsylvanicus. 19714254496
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. v. structure of the galactosyldiglycerides. 19724342947
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. vii. structure of the phosphogalactolipids. 19744367972
[the amino acid sequence of the serine and aspartic acid containing mureins of bifidobacterium bifidum orla jensen]. 19704395418
growth of bifidobacterium bifidum n4 in solid medium limited by exogenous lactose. 19744431102
the production of bisulfite from pantetheine-s-sulfonic acid by bifidobacterium bifidum. 19744434589
growth responses of bifidobacterium bifidum to s-sulfonic acid-type pantetheine related compounds. 19724538355
a capillary tube method for counting viable cells of bifidobacterium bifidum grown in a solid medium. 19744610237
[proceedings: intestinal implantation of bifidibacterium bifidum in the adult--study of a new technic of preparation for gastrointestinal surgery]. 19744831250
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell-wall inhibition. vi. biosynthesis of the galactosyldiglycerides. 19744838219
[possibilities of a physiological antibiotic therapy in the infant with bacterium bifidum (lactobacillus bifidus)]. 19664861947
[fecal bacterial flora in an infant without bacterium bifidum--presence of bacterium bifidus-inhibiting spherocytes]. 19694898357
influence of nutrition on the morphology of a strain of bifidobacterium bifidum.a mucoid variant of bifidobacterium bifidum was converted from its normal curved rod or bifid form to a highly branched form when grown in a chemically defined minimal medium. branching could be prevented by the addition of a mixture of dl-alanine, dl-aspartic acid, l(+)-glutamic acid, and dl-serine, but not when any one of these four amino acids was omitted. although sodium chloride induced pleomorphism, calcium ions were ineffective in suppressing the appearance of these pleomorphic forms. non ...19725053884
[bacillus bifidus in the bacterial flora and its role in the prevention of enterocolitis in the newborn]. 19705205589
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. iv. galactolipid composition. 19715282823
growth responses of bifidobacterium bifidum to coenzyme a, its precursors and carrot extract. 19685304593
[trial of bacillus bifidus implantation in the healthy infant and in a carrier of pathogenic escherichia coli (preliminary note)]. 19655322986
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. 3. morphological structure and osmotic properties of the protoplasts and membrane composition. 19705473501
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. ii. fatty acid composition. 19705476260
[bacterial enterocolitis in an infant. treatment with oral bacillus bifidus culture]. 19705522806
mechanism of utilization of pantetheine-s-sulfonic acid by bifidobacterium bifidum. 19715567062
[a new culture medium for the isolation and maintenance of bifidobacterium bifidum (lactobacillus bifidus)]. 19675585009
[on the metabolism of bacterium bifidum (lactobacillus bifidus). xii. the production of propionic acid]. 19675586072
[consideration on the treatment of acute and chronic enterocolopathies by lyophilized bacillus bifidus mized extemporaneously with its growth factors. a propos of 100 cases]. 19675594645
[on the metabolism of bacterium bifidum. (lactobacillus bifidus). x. production of ethanol]. 19675594990
[supplementary findings on vitamin b1 in pediatrics. 2. vitamin b1--producting capacity of bacillus bifidus and effect of bifidus preparationson the vitamin b1 levels of the blood and urine]. 19675625889
fermentation of glucose, lactose, galactose, mannitol, and xylose by bifidobacteria.for six strains of bifidobacterium bifidum (lactobacillus bifidus), fermentation balances of glucose, lactose, galactose, mannitol, and xylose were determined. products formed were acetate, l(+)-lactate, ethyl alcohol, and formate. l(+)-lactate dehydrogenase of all strains studied was found to have an absolute requirement for fructose-1,6-diphosphate. the phosphoroclastic enzyme could not be demonstrated in cell-free extracts. cell suspensions fermented pyruvate to equimolar amounts of acetate a ...19685674058
the effect of bacterium bifidum on intestinal bacterial flora and toxic protein metabolites in chronic liver disease. 19685679810
predicting the stability of freeze-dried lacto-bacillus bifidus by the accelerated storage test. 19685717948
uptake and metabolism of derivatives of 2-deoxy-2-amino-d-glucose in bifidobacterium bifidum var. pennsylvanicus. 19695762966
biochemical changes in bifidobacterium bifidum var. pennsylvanicus after cell wall inhibition. i. composition of lipids. 19695766029
[on metabolism of bacterium bifidum(lactobacillus bifidus). 8. typing of our strains]. 19655887810
[on metabolism of bacterium bifidum(lactobacillus bifidus). vii. study of the formation of amino acids]. 19655899410
[metabolic exchange between microorganism and macroorganism with the example of bacterium bifidum]. 19665987113
antibacterial sensitivity of bifidobacterium (lactobacillus bifidus).the antibacterial sensitivity patterns of gram-positive, nonsporeforming, anaerobic bacilli variously classed as lactobacillus bifidus, actinomyces bifidus, or bifidobacterium were studied by the plate dilution method. a total of 34 strains, mostly from human feces, was studied. three species, b. longum, b. adolescentis, and b. bifidum, were represented with 11, 11, and 6 strains, respectively. the other six strains fell into four other species. most strains of all types resisted 100 mug/ml or m ...19676020399
pathway of glucose fermentation in relation to the taxonomy of bifidobacteria.cell-free extracts of 17 strains of bifidobacterium bifidum (lactobacillus bifidus) were examined for the presence of aldolase, glucose-6-phosphate dehydrogenase, and fructose-6-phosphate phosphoketolase. all strains turned out to lack aldolase, an enzyme unique to glycolysis, and glucose-6-phosphate dehydrogenase, characteristic of the hexosemonophosphate pathway. in all strains, fructose-6-phosphate phosphoketolase could be demonstrated. it can be concluded that bifidobacteria ferment glucose ...19676020562
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