Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of intestinal bacteria on incidence of liver tumors in gnotobiotic c3h/he male mice. | the effect of intestinal microflora on liver tumorigenesis was studied in gnotobiotic c3h/he male mice monoassociated, diassociated, or polyassociated with the following strains of intestinal bacteria: escherichia coli, streptococcus faecalis, bifidobacterium adolescentis, bifidobacterium infantis, clostridium indolis, c. paraputrificum, c. perfringens, c. innocuum, c. nexile, c. ramosum, c. clostridiiforme, bacteroides multiacidus, bacteroides fragilis, veillonella alcalescens, v. parvula, and ... | 1979 | 292809 |
| [antibacterial and bactericidal activity of tinidazole against anaerobic bacteria comparing with metronidazole (author's transl)]. | antibacterial activity of tinidazole (1-2-(ethylsulfonyl)-2-methyl-5-nitroimidazole) against anaerobic bacteria including peptococcus, peptostreptococcus, eubacterium, propionibacterium, bacteroides and fusobacterium was studied by agar dilution method comparing with metronidazole. in addition to this work, bactericidal effect of tinidazole and metronidazole against p. prevotii, b. fragilis ss. fragilis and f. varium was examined by quantitative culture method after incubation in gam broth conta ... | 1976 | 1271586 |
| species-specific oligonucleotide probes for five bifidobacterium species detected in human intestinal microflora. | portions of the 16s rrna from closely related species of the genus bifidobacterium that are found in the human intestinal microflora were sequenced in order to design species-specific oligonucleotide probes. five oligonucleotide probes ranging from 16 to 19 bases in length and complementary to 16s rrna sequences from bifidobacterium adolescentis, b. bifidum, b. breve, b. infantis, and b. longum were synthesized. with crude high-molecular-weight rna preparations as targets, these probes showed th ... | 1992 | 1282315 |
| relationship between oxygen sensitivity and oxygen metabolism of bifidobacterium species. | bifidobacteria, which are obligate anaerobes, were studied to determine the relationship between their sensitivity to oxygen and oxygen metabolism. among the four species tested, bifidobacterium infantis, bifidobacterium breve, and bifidobacterium longum differed from bifidobacterium adolescentis in sensitivity to oxygen. the former three species showed marked growth under conditions of partial aeration, whereas the growth of b. adolescentis was suppressed by low concentrations of oxygen. bifido ... | 1992 | 1474198 |
| influence of decontamination on induction of arthritis in lewis rats by cell wall fragments of eubacterium aerofaciens. arthropathic properties of indigenous anaerobic bacteria. | although the cause (or causes) of rheumatoid arthritis is unknown, many workers have suggested that microorganisms play a part. the intestinal flora in particular has been related to the development of joint inflammation. it has been shown previously that cell wall fragments of several anaerobic gram positive intestinal bacteria of human origin are arthritogenic after a single intraperitoneal injection in lewis rats. the part played by indigenous microflora in this model has now been studied by ... | 1992 | 1586251 |
| occurrence of bifidobacteria in the stool of multiple sclerosis patients. | stool specimens from 17 multiple sclerosis (ms) patients were examined for bifidobacteria. the count of colony forming units (cfu)/g faeces and the differentiation and typing of species, especially of bifidobacterium adolescentis biotypes were performed. the ph of the samples was also measured. stool specimens from 17 healthy volunteers were examined as a control by the same methods (a modified medium, suitable for the detection and recognition of bifidobacteria in adults is given). the results ... | 1991 | 1755926 |
| the role of escherichia coli haemolysin in the pathogenic synergy of colonic bacteria in subcutaneous abscess formation in mice. | the growth of nine species of colonic bacteria--escherichia coli, enterococcus faecalis, bacteroides ovatus, fusobacterium varium, clostridium perfringens, klebsiella pneumoniae, proteus vulgaris, staphylococcus aureus and bifidobacterium adolescentis--was examined after concomitant injection to form experimental subcutaneous abscesses in mice. injection of a mixture of c. 10(5) cfu of each of the first five strains (e. coli, ent. faecalis, b. ovatis, f. varium and c. perfringens) resulted in ab ... | 1990 | 2146395 |
| effect of rice fiber on human fecal microflora. | the effects of brown rice, containing fourfold as much dietary fiber as polished rice, on the human fecal microflora were determined. significantly increased numbers of bifidobacterium adolescentis and enterococcus faecalis were observed during the brown rice intake, whereas the total counts and the numbers of bacteroides, eubacterium aerofaciens, and escherichia coli during the intake were lower than those before and after the intake. lower numbers of clostridia and low incidences of clostridiu ... | 1989 | 2547140 |
| comparison of fecal microflora of elderly persons in rural and urban areas of japan. | the fecal microflora of 15 healthy elderly persons with a median age of 84 years in a rural area whose inhabitants tend to be long-lived (yuzurihara-area, uenohara, yamanashi prefecture) was compared with the microflora of individuals with a median age of 68 years in an urban area (tokyo). the diet of the elderly persons in the yuzurihara area is characterized by a high intake of dietary fiber. total numbers of anaerobic bacteria were significantly smaller in the elderly persons in the yuzurihar ... | 1989 | 2547333 |
| structure of 6-deoxytalose-containing polysaccharide from the cell wall of bifidobacterium adolescentis. | the isolation and analysis of the cell wall and the polysaccharide-glycopeptide complexes of bifidobacterium adolescentis yit4011 are presented. polysaccharide-glycopeptide complexes, ps-gp1 and ps-gp2, were solubilized from the cell wall by treatment with n-acetylmuramidase. ps-gp1 and ps-gp2 were found to be composed of glucose, 6-deoxytalose and a small amount of glycopeptide. the products of smith degradation of the ps-gps had no glucose-containing fraction, but were composed of 1,2/1,3-link ... | 1988 | 3170504 |
| growth-enhancing supplements for various species of the genus bifidobacterium. | various biological materials were tested for their growth-promoting activity of several bifidobacterial species in a synthetic medium containing ample sources of inorganic salts, vitamins, nitrogen, and carbon. it was found that only bifidobacterium adolescentis and b. longum (atcc 15708) grew optimally or near optimally in the synthetic medium. all the other bifidobacteria tested grew optimally only in the synthetic medium supplemented with a growth promoter. the best growth promoters for all b ... | 1988 | 3235728 |
| comparison of the fecal microflora in rural japanese and urban canadians. | the fecal microflora of nine rural healthy japanese and eight urban healthy canadians was examined. the two populations ate typical japanese and western diets, respectively. the numbers of eubacteria (p less than 0.01), bifidobacteria (p less than 0.05), bacilli (p less than 0.01), lactobacilli and veillonellae and the frequency of occurrence of bifidobacteria were higher in the japanese than in the canadians. higher numbers of bacteroides and lecithinase-negative clostridia were found in the ca ... | 1986 | 3747865 |
| distribution of metronidazole susceptibility factors in obligate anaerobes. | the distribution of metronidazole susceptibility factors in clinically important bacteria was studied to determine why obligate anaerobes were susceptible to metronidazole and facultative ones were not. with obligate and facultative anaerobes, nitroreductase activity, metronidazole uptake, pyruvate:ferredoxin oxidoreductase activity and drug susceptibility were estimated. nitroreductase activity was distributed in the obligate anaerobes and some facultative ones such as streptococcus faecalis. p ... | 1986 | 3804881 |
| survival and enumeration of the fecal indicators bifidobacterium adolescentis and escherichia coli in a tropical rain forest watershed. | the density of bifidobacterium spp., fecal coliforms, escherichia coli, and total anaerobic bacteria, acridine orange direct counts, percentages of total bacterial community activity and respiration, and 12 physical and chemical parameters were measured simultaneously at six sites for 12 months in the mameyes river rain forest watershed, puerto rico. the densities of all bacteria were higher than those reported for uncontaminated temperate rivers, even though other water quality parameters would ... | 1985 | 3901921 |
| exopeptidase profiles of bifidobacteria. | the exopeptidase activities of five different strains of bifidobacteria occurring habitually in healthy human intestinal canal were measured on 61 synthetic substrates. the cluster analysis, based on the results, indicates that four strains, with the exception of bifidobacterium adolescentis a m101-4, have similar exopeptidase profiles. all cfe from these five strains contained at least three kinds of aminopeptidases (aminopeptidase with broad substrate specificity, aminopeptidase hydrolyzing se ... | 1985 | 3914536 |
| [actinomyces eriksonii georg, robertstad, brinkman and hicklin 1965 identical with bifidobacterium adolescentis reuter 1963 (author's transl)]. | 1974 | 4151212 | |
| predominant gram-positive bacteria in human feces: numbers, variety, and persistence. | the predominant gram-positive bacteria in 47 fecal specimens from 10 healthy men were studied by microscopic and cultural counts, by the characterization and tentative identification of isolates, and by the use of fluorescein isothiocyanate (fitc)-conjugated globulins prepared using some of the isolates. gram-positive bacteria averaged 10(10.5+/-0.4(sd)/g (wet weight) of feces with significant variation from host to host. characterization of 865 isolates, all strict anaerobes and carbohydrate fe ... | 1974 | 4595760 |
| experimental shigella infections in laboratory animals. i. antagonism by human normal flora components in gnotobiotic mice. | germfree mice were associated with selected species of human intestinal bacteria and then challenged with a streptomycin-resistant shigella flexneri strain. antagonism against shigella was most pronounced in mice associated with escherichia coli and least pronounced in mice associated with bacteroides fragilis. a moderate degree of antagonism could be demonstrated in mice associated with either streptococcus faecalis or bifidobacterium adolescentis. shigella persisted in the cecal contents of e. ... | 1972 | 4631914 |
| [bacteria and dietary fiber]. | the presence of cellulose particles, so in-vitro studies have shown, influences the growth rate and metabolic activity of the bacteria escherichia coli, bifidobacterium adolescentis and chromobacterium violaceum. after the homogenization in waring blendor, the growth rate has been found to be highest in a medium containing 10% cellulose particles and to show a decreasing tendency in media containing 5%, 1% and 0% cellulose. the biochemical intensity, judged by glucose consumption and intensity o ... | 1984 | 6326399 |
| bacterial formation of aldosterone metabolites. | the experiments described in this paper demonstrate that most of the metabolic alterations of the aldosterone molecule, hitherto attributed to hepatic enzymes, equally well may be carried out by enzymes synthesized by anaerobic bacteria from the human gut. the steroid reductases synthesized by clostridium paraputrificum, clostridium j-1, and clostridium innocuum convert aldosterone to the 3 alpha, 5 beta tetrahydroaldosterone (tha), 3 beta, 5 alpha-tha, and 3 alpha, 5 alpha-tha, respectively. al ... | 1984 | 6513554 |
| sorbitol-fermenting bifidobacteria as specific indicators of human faecal pollution. | bifidobacteria were consistently present in the faeces of both man and pigs but only occasionally in the faeces of cattle and sheep, and they were not isolated from faecal samples from other animals; total counts of bifidobacteria were obtained by membrane filtration with yn-17 medium, a modification of resnick and levin's yn-6 medium. mannitol-fermenting strains of bifidobacteria were isolated from both human and animal faeces, but sorbitol-fermenting strains were obtained only from human sampl ... | 1983 | 6654767 |
| biotransformation of 1-nitropyrene in intestinal anaerobic bacteria. | mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ... | 1982 | 7167066 |
| formation of 20 beta-dihydrosteroids by anaerobic bacteria. | cortisol was metabolized to a variety of products, among them small amounts of cortol by fecal flora of humans and rats. a microorganism, bifidobacterium adolescentis, isolated from both sources, synthesized a 20-hydroxysteroid dehydrogenase which reduced cortisol to 20 beta-dihydrocortisol. the metabolite was reduced to cortol by clostridium paraputrificum. the 20-hydroxysteroid dehydrogenase showed a wide substrate specificity; it was independent of the 4-ene and the configuration at c3, c-11, ... | 1982 | 7176656 |
| pyelonephritis and septicemia due to gram-positive rods similar to corynebacterium group e (aerotolerant bifidobacterium adolescentis). | 1980 | 7387006 | |
| urinary tract infection caused by anaerobic bacteria in children. | anaerobic bacteria were recovered from 5 children with urinary tract infection (uti). three had pyelonephritis and 2 cystitis. two of the patients had a history of prior recurrent uti. urine samples were collected using suprapubic aspiration. the anaerobic organism recovered were 3 isolates of bacteroides fragilis and one each of b. melaninogenicus, peptococcus asaccharolyticus, and bifidobacterium adolescentis. mixed infection was present in 3 children. in 2 cases b. fragilis were present with ... | 1980 | 7445306 |
| [architectonics of bifidobacteria populations: submicroscopic aspect of cell cohesion in bifidobacterium adolescentis and bifidobacterium bifidum]. | populations (cultures) of bifidobacterium adolescents and b. bifidum, growing on artificial liquid and agar media, are presented by highly ordered mycelial structures. the topography of them depends on mutual arrangement of polymorphic cells and the way of their daughter cells separation after division. evidences obtained by scanning electron microscopy (sem) of total preparations and by transmission electron microscopy (tem) of ultrathin sections correlate well. these data showed the existence ... | 1995 | 7616878 |
| purification and characterization of d-xylose isomerase from bifidobacterium adolescentis. | d-xylose isomerase was purified to homogeneity from cell-free extracts of bifidobacterium adolescentis by ammonium sulfate fractionation and chromatographies on deae-cellulose and butyl-toyopearl. the molecular weight of the purified enzyme was estimated to be 168,000 by gel filtration on tskgel g-3000sw, and 53,000 on sds-polyacrylamide gel electrophoresis. the optimum ph was around 7 and the enzyme was stable at ph 7-8. the enzyme required bivalent cations, mg2+, co2+, or mn2+ for the activity ... | 1994 | 7764860 |
| absence of cholic acid 7 alpha-dehydroxylase activity in the strains of lactobacillus and bifidobacterium. | to investigate the presence of 7 alpha-dehydroxylase activity on bile acids in the bacterial strains of fermented milk products, 46 strains of lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus gasseri, bifidobacterium adolescentis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactococcus lactis spp. lactis, and streptococcus salivarius spp. thermophilus were tested for their ability ... | 1994 | 7814703 |
| biotin production by bifidobacteria. | biotin production and the growth of the strains of bifidobacterium adolescentis, b. bifidum, b. breve, b. infantis, and b. longum were studied. these five strains showed heavy growth on bl medium. but when yeast extract medium (carbon source, glucose) was used, the extent of their growth was significantly decreased, one-half or less than that of the growth on bl medium. b. bifidum grew well on yeast extract medium containing oligosaccharides, such as isomaltooligosaccharide, and produced biotin ... | 1994 | 7931726 |
| growth stimulator for bifidobacteria produced by propionibacterium freudenreichii and several intestinal bacteria. | a bifidogenic growth stimulator was present in the cell-free filtrate of propionibacterium freudenreichii 7025 culture and in the methanol extract fraction of the cells. several intestinal bacteria, such as bacteroides, enterobacter, and enterococcus, which also released a growth stimulator for bifidobacteria, may play an important role in regulation of a bifidobacterial population in colonic microflora. the water-soluble stimulator from the methanol extract of the cells was partially purified. ... | 1994 | 8182163 |
| [actinomycosis infection of the cerebellum]. | the case is presented of a 37-year-old male with a cerebellar abscess due to actinomyces israelii. the patient was admitted after dental surgery because of fever and symptoms indicative of raised, intracranial pressure. no abnormalities were detected in peripheral blood and cerebrospinal fluid. ct scan revealed a space-occupying lesion in the left cerebellar hemisphere with perifocal oedema and occlusive hydrocephalus. after emergency external ventriculostomy complete removal of the mass was ach ... | 1993 | 8333207 |
| analysis of intestinal flora of a patient with congenital absence of the portal vein. | a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ... | 1993 | 8364525 |
| role of intestinal bacteria in ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug. | the role of intestinal bacteria in induction and repression of ulcer formation in the ileum of rats treated with one of the nonsteroidal antiinflammatory drugs (nsaids), 5-bromo-2-(4-fluorophenyl)-3-(4-methylsulfonylphenyl) thiophene (bfmet), was examined in this study. bfmet was administered by intragastric gavage once at doses of 500-1,500 mg/kg of body weight to wistar rats treated with and without antibiotics (bacitracin, neomycin, streptomycin), germ-free rats and gnotobiotic rats, and 72 h ... | 1996 | 8887349 |
| pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. | pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ... | 1996 | 8919784 |
| a partially purified beta-glucosidase from bifidobacterium adolescentis converts cycasin to a mutagenic compound. | beta-glucosidase was extracted from sonicated bifidobacterium adolescentis int-57 and partially purified by sepharose cl-6b gel-filtration and deae-cellulose ion-exchange chromatography. the partially purified enzyme was confirmed to convert cycasin to a mutagen in the ames and sos chromotests. beta-glucosidase negative strains were unable to activate cycasin mutagenically. | 1996 | 8936375 |
| stereochemical course of hydrolysis catalyzed by arabinofuranosyl hydrolases. | the stereochemical course of hydrolysis catalyzed by various enzymes acting on arabinofuranosyl linkages has been determined. 1h-nmr analysis of the action of endo-(1-->5)-alpha-l-arabinanases from aspergillus niger and aspergillus aculeatus showed that both hydrolyze linear arabinan with inversion of configuration, and may therefore act via a single displacement mechanism. this is consistent with the a. niger enzyme's classification in glycosyl hydrolase family 43. the catalytic mechanisms of a ... | 1996 | 8946944 |
| characterization of a water-soluble polysaccharide fraction with immunopotentiating activity from bifidobacterium adolescentis m101-4. | the soluble and insoluble fractions obtained after sonication and centrifugation of bifidobacterium adolescentis m101-4 cells were examined, and both of these fractions exhibited mitogenic activity in an assay of murine splenocytes and peyer's patch cells in vitro. the soluble fraction was further treated by a 6-step procedure involving proteinase k-treatment, ultrafiltration with a 50-kda cut-off molecular-sieving membrane, anion-exchange chromatography, dialysis, ultrafiltration through a 6-kd ... | 1997 | 9058970 |
| collagen binding of bifidobacterium adolescentis. | in this study, 13 bifidobacterial strains were tested for their ability to adhere to immobilized extracellular matrix (ecm) proteins. only two bifidobacterium adolescentis strains adhered to immobilized type i and type v collagens, but not to laminin, fibronectin, and type iii and iv collagens. the adhesion of b. adolescentis bb-119 to type v collagen was inhibited by type i and v collagens and gelatin, and was diminished after protease treatment of the cells. periodate treatment of immobilized ... | 1997 | 9099635 |
| a new arabinofuranohydrolase from bifidobacterium adolescentis able to remove arabinosyl residues from double-substituted xylose units in arabinoxylan. | an arabinofuranohydrolase (axh-d3) was purified from a cell-free extract of bifidobacterium adolescentis dsm 20083. the enzyme had a molecular mass of approximately 100 kda as determined by gel filtration. it displayed maximum activity at ph 6 and 30 degrees c. using an arabinoxylan-derived oligosaccharide containing double-substituted xylopyranosyl residues established that the enzyme specifically released terminal arabinofuranosyl residues linked to c-3 of double-substituted xylopyranosyl resi ... | 1997 | 9114514 |
| digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples. | the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ... | 1997 | 9241587 |
| effect of intestinal bacteria on formation of azoxymethane-induced aberrant crypt foci in the rat colon. | the effect of intestinal bacteria on formation of azoxymethane (aom)-induced aberrant crypt foci (acf) and dna adducts in the rat colon was investigated. male sprague-dawley rats were administered cultures of lactobacillus acidophilus, bifidobacterium adolescentis, bacteroides fragilis, escherichia coli and clostridium perfringens for five weeks and given injections of aom at 15 mg/kg body weight at the first and second weeks. the number of acf five weeks after the start of the experiment was de ... | 1997 | 9325162 |
| note: purification of amylase secreted from bifidobacterium adolescentis. | bifidobacterium adolescentis int-57 isolated from human faeces produced extracellular amylase. the enzyme was purified from the culture supernatant fluids by ammonium sulphate precipitation, gel-filtration chromatography (sephadex-g-75), ion-exchange chromatography (cm-cellulose) and fplc. sds-page of the purified enzyme revealed a major band with an apparent molecular weight of 66 kda. the pi was 5.2. enzyme activity was optimal at 50 degrees c, and at ph 5.5. the enzyme was stable at 20-40 deg ... | 1997 | 9351206 |
| [isolation and characteristics of a protein-polysaccharide complex, secreted by bifidobacterium adolescentis]. | extracellular fibrillar protein-polysaccharide complex (ppc) of bifidobacterium adolescentis was found to be bound to the outer surface of the cell wall. however, at the late stages of b. adolescentis cultivation (48-72 h of growth), it also occurred in the culture liquid, which can be explained by the expansion of the outer material of the cell wall. ppcs isolated from the cell surface and culture liquid contained proteins and carbohydrates in a ratio of 1:1 and from 1:4 to 1:5, respectively. p ... | 1997 | 9424559 |
| [biological activity of the protein-polysaccharide complex secreted by bifidobacterium adolescentis]. | extracellular protein-polysaccharide complex (ppc) of bifidobacterium adolescentis stimulated growth, acidogenesis, accumulation of extracellular proteins and enzymes and the utilization of sugars in both physiologically active and anabiotic forms of b. adolescentis cultivated in rich nutrient media. the ppc variants iii-dn-48 and iv-dn-24 isolated from the cell surface of b. adolescentis had the most pronounced bifidogenic effect. ppc promoted a quick and complete rehabitation of anabiotic bifi ... | 1997 | 9424560 |
| [features of growth and formation of extracellular proteinases of bifidobacterium adolescentis 94-bim]. | growth characteristics of a new bifidobacterial strain, bifidobacterium adolescentis 94-bim, were determined during cultivation in various nutrient media. the strain was obtained through selection by the criterion of proteolytic activity and was shown to synthesize acidic, neutral, and alkaline extracellular proteinases in all of the growth media tested. the growth yield of the strain varied from 2.9 x 10(8) to 1.7 x 10(10) cfu/ml with acidogenesis of up to 131 degrees t. the most rapid growth a ... | 1997 | 9424561 |
| culture supernatants of lactobacillus acidophilus and bifidobacterium adolescentis repress ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug by suppressing unbalanced growth of aerobic bacteria and lipid peroxidation. | a nonsteroidal antiinflammatory drug, 5-bromo-2-(4-fluorophenyl)-3-(4-methylsulfonylphenyl) thiophene (bfmet), induced ileal ulcers in rats after oral administration, while no ulcers were observed after subcutaneous injection. the ileal ulcer formation in bfmet-treated rats was examined to correlate the administration of cultures of lactobacillus acidophilus or bifidobacterium adolescentis with intestinal bacteria in the ileal contents and lipid peroxidation of the small intestinal mucosa. ileal ... | 1998 | 9654366 |
| stimulation of cytokine production in clonal macrophage and t-cell models by streptococcus thermophilus: comparison with bifidobacterium sp. and lactobacillus bulgaricus. | the effects of four commercial strains of streptococcus thermophilus used in yogurt manufacturing on cytokine production were evaluated by using a macrophage model (raw 264.7 cells) and a t-helper-cell model (el4.il-2 thymoma cells) and compared to immunologically active strains of lactobacillus bulgaricus, bifidobacterium adolescentis, and bifidobacterium bifidum. all cytokines (tnf-alpha and il-6 in raw 264.7 cells and il-2 and il-5 in el4.il-2 cells) were affected by heat-killed s. thermophil ... | 1998 | 9678170 |
| [structure-functional organization of bifidobacteria]. | regularities in the development of bifidobacterial populations were studied at cellular and subcellular levels. the population development of bifidobacterium adolescentis mc-42 and b. bifidum no. 1 was shown to be accompanied by morphological cell differentiation. a scheme of morphological transformations of bifidobacterial cells in the developmental cycle of populations is proposed. multiplication occurs via reversion of transitory rod-shaped and coccoid forms into repeatedly budding and dichot ... | 1998 | 9702729 |
| growth-inhibitory effects of galla rhois-derived tannins on intestinal bacteria. | the growth-inhibitory activity of galla rhois-derived materials towards 17 intestinal bacteria was evaluated using an impregnated paper disc method. the biologically active components of galla rhois were characterized as the tannins methyl gallate (mg) and gallic acid (ga) by spectral analysis. the growth responses varied with bacterial strain tested. in the test using 10 mg disc-1, mg and ga produced a clear inhibitory effect on harmful bacteria such as clostridium perfringens, cl. paraputrific ... | 1998 | 9721649 |
| characterization of bifidobacteria by random dna amplification. | rapd conditions were optimized to generate reproducible banding patterns by testing primers, thermocyclers and overall reproducibility in repeat dna analysis and separate dna extractions. five primers were chosen on the basis of band intensity and distribution (between 2 and 10 bands) which clearly distinguished among strains of bifidobacterium adolescentis, b. animalis, b. bifidum, b. breve, b. infantis and b. longum. the use of five single-primer reactions under optimized conditions improved t ... | 1998 | 9801194 |
| rapid identification of human intestinal bifidobacteria by 16s rrna-targeted species- and group-specific primers. | on the basis of 16s rrna sequences, species- and group-specific primers for bifidobacterium adolescentis, b. angulatum, b. bifidum, b. breve, the b. catenulatum group (b. catenulatum and b. pseudocatenulatum), and the b. longum group (b. longum and b. infantis), which are species commonly found in human intestinal tracts, were developed. the specificity of these primers was confirmed through the use of dna extracted from 46 strains of 31 bifidobacterium species, as well as 14 non-bifidobacterial ... | 1998 | 9809413 |
| alpha-galactosidase of bifidobacterium adolescentis dsm 20083. | bifidobacterium adolescentis was grown anaerobically in medium enriched with alpha-d-galactosides. alpha-galactosidase (ec 3.2.1. 22) was released from the cells by ultrasonic treatment and purified 36-fold by ultrafiltration, ammonium-sulphate precipitation, anion-exchange chromatography, and size-exclusion chromatography. two protein bands were consistantly observed after sodium-dodecylsulfate polyacrylamide gel electrophoresis (sds-page). electrophoretically homogeneous alpha-galactosidase wa ... | 1999 | 9871108 |
| production and shelf-life studies of low cost beverage with soymilk, buffalo cheese whey and cow milk fermented by mixed cultures of lactobacillus casei ssp. shirota and bifidobacterium adolescentis. | a study was performed to develop a fermented milk beverage with the aim to increase the potential application of buffalo cheese whey and soymilk. a mixed substrate was prepared by selective combination, which contained buffalo cheese whey 35%, soymilk 30% and cow milk 35%. the substrate mixture was fermented by a mixed culture of lactobacillus casei shirota and bifidobacterium adolescentis at 37 degrees c for 8 h keeping a 1:1.5 proportion between the lactic and bifidobacteria within a 5% (v/v) ... | 1999 | 10520270 |
| growth-inhibiting effects of coptis japonica root-derived isoquinoline alkaloids on human intestinal bacteria. | the growth-inhibiting activity of coptis japonica (makino) root-derived materials toward eight human intestinal bacteria was examined using an impregnated paper disk method and compared to that of four commercially available isoquinoline alkaloids [berberine sulfate (bs), berberine iodide (bi), palmatine chloride (pc), and palmatine sulfate(ps)], as well as that of thea sinensis leaf-derived epigallocatechin gallate (egcg). the biologically active constituents of the coptis extract were characte ... | 1999 | 10552394 |
| transglycosidase activity of bifidobacterium adolescentis dsm 20083 alpha-galactosidase. | bifidobacterium adolescentis, a gram-positive saccharolytic bacterium found in the human colon, can, alongside other bacteria, utilise stachyose in vitro thanks to the production of an alpha-galactosidase. the enzyme was purified from the cell-free extract of bi. adolescentis dsm 20083t. it was found to act with retention of configuration (alpha-->alpha), releasing alpha-galactose from p-nitrophenyl galactoside. this hydrolysis probably operates with a double-displacement mechanism, and is consi ... | 1999 | 10570815 |
| sorbitol-fermenting bifidobacteria as indicators of diffuse human faecal pollution in estuarine watersheds. | sorbitol fermenting bifidobacteria were evaluated as indicators of non-point source human faecal pollution to three sub-estuaries with elevated faecal coliform densities. human-specific bifidobacteria correlated with identifiable human sanitary deficiencies in feeder streams to estuarine creeks in two of three watersheds examined, one rural and one moderately developed. sorbitol-fermenting bifidobacteria were recovered at densities ranging from 1 to 90 colony-forming-units 100 ml-1 in 11 of 258 ... | 1999 | 10583680 |
| metabolization of beta-(2,6)-linked fructose-oligosaccharides by different bifidobacteria. | low-molecular-mass beta-(2,6)-linked fructose-oligosaccharides (beta-(2,6)-fos) were examined as a new carbohydrate source for growth of bifidobacteria. beta-(2,6)-fos were prepared from microbial high-molecular-mass levan by acid hydrolysis and refined by cation-exchange chromatography. (13)c-nmr spectroscopy confirmed the presence of predominantly beta-(2,6)-fructosyl linkages in the oligosaccharides. more than 80% beta-(2,6)-fos was recovered after in vitro incubation with amylolytic and prot ... | 2000 | 10612749 |
| characterization of a novel beta-galactosidase from bifidobacterium adolescentis dsm 20083 active towards transgalactooligosaccharides. | this paper reports on the effects of both reducing and nonreducing transgalactooligosaccharides (tos) comprising 2 to 8 residues on the growth of bifidobacterium adolescentis dsm 20083 and on the production of a novel beta-galactosidase (beta-gal ii). in cells grown on tos, in addition to the lactose-degrading beta-gal (beta-gal i), another beta-gal (beta-gal ii) was detected and it showed activity towards tos but not towards lactose. beta-gal ii activity was at least 20-fold higher when cells w ... | 2000 | 10742215 |
| [production of the therapeutic and prophylactic preparation enterobifidin on the basis of bifidobacterium adolescentis mc-42]. | production of enterobifidin comprises preparation of culture media, reparation of lyophilized bifidobacterium adolescentis ms-42 culture, preparation of starters, cultivation of bacteria in fermenters, biomass conservation, and its biological control. the preparation contains physiologically active bifidobacterium cells with high activities of growth (mu = 0.7 h-1, g = 1.0 h) and acid formation (titratable acidity is approximately 120-140 degrees t; acetate concentration, 0.50-0.75%; and lactate ... | 2000 | 10752094 |
| cordycepin: selective growth inhibitor derived from liquid culture of cordyceps militaris against clostridium spp. | the growth responses of nine human intestinal bacteria to liquid culture of cordyceps militaris link. pt. (ascomycotina: clavicipitaceae) collected from a pupa of bombyx mori l. (lepidoptera: bombycidae) were examined using spectrophotometric and impregnated paper disk methods and compared to those of tetracycline and chloramphenicol, as well as those of coptis japonica root-derived berberine chloride. the biologically active constituent of the cultures was characterized as cordycepin (3'-deoxya ... | 2000 | 10898616 |
| [the action of a preparation of escherichia coli m-17 growth autostimulants (actoflor) on the growth of pure and mixed bacterial cultures]. | the effect of the preparation of e. coli m-17 low-molecular exometabolites (actoflor), containing growth autostimulators, on the growth of pure cultures of e. coli m-17 e. coli k-12, salmonella enteritidis, serratia marcescens and bifidobacterium adolescentis mc-42 was studied. this preparation was shown to stimulate the growth of all above-mentioned bacteria. the addition of actoflor also led to the acceleration of growth in the cultivation of mixed cultures of e. coli m-17 with e. coli k-12 (o ... | 2000 | 10925866 |
| evaluation of 16s rrna and cellular fatty acid profiles as markers of human intestinal bacterial growth in the chemostat. | chemostats were used to study the effects of carbon and nitrogen limitation and specific growth rate on 16s rrna synthesis and cellular fatty acid (cfa) profiles in four human intestinal bacteria (bacteroides thetaiotaomicron, bifidobacterium adolescentis, clostridium bifermentans and cl. difficile). cellular fatty acid synthesis varied with dilution rate and nutrient availability in different species, but these cellular constituents were relatively stable phenotypic characteristics in bact. the ... | 2000 | 11054172 |
| bifidobacterial diversity in human feces detected by genus-specific pcr and denaturing gradient gel electrophoresis. | we describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on pcr and denaturing gradient gel electrophoresis (dgge). bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16s ribosomal dna (rdna), and the fragments were separated in a sequence-specific manner in dgge. pcr products of the same length from different bifidobacterial species showed good separation upon dgge. dgge of ... | 2001 | 11157210 |
| comparison of mucosal adhesion and species identification of bifidobacteria isolated from healthy and allergic infants. | fifty bifidobacteria strains were isolated from fecal samples of allergic and age matched healthy infants. allergic infants were found to have an adult type bifidobacterium flora with high levels of bifidobacterium adolescentis. healthy infants had a typical infant bifidobacterium flora with high levels of bifidobacterium bifidum. these isolates were tested for their adhesive properties to human intestinal mucus. the adhesion of the fecal bifidobacteria from healthy infants was significantly hig ... | 2001 | 11172990 |
| [production of proteinases associated with bifidobacterium adolescentis 94-bim cell wall]. | bifidobacterium adolescentis 94-bim was found to produce cell-wall bound proteolytic enzymes active at acidic, neutral, and alkaline ph values. the solubilization of proteinases with 0.5% triton x-100 substantially improved the yield of the enzymes. the most active accumulation of cell-bound proteinases was observed in the third hour of cultivation at rates of 156.7, 179.5, and 111.1 u/(mg h), measured at ph 2.5, 7.0, and 9.0, respectively. it is suggested that the cell-wall bound proteinases of ... | 2000 | 11195575 |
| bifidobacterium adolescentis modulates the specific immune response to another human gut bacterium, bacteroides thetaiotaomicron, in gnotobiotic rats. | in order to investigate the capability of an autochthonous bacterium to modulate the host's immune response against the indigenous microfiora, the immunogenicity of two selected bacterial species of the human gut was investigated in a gnotobiotic rat model. germ-free (gf) rats were monoassociated with either bifidobacterium (b.) adolescentis or bacteroides (b.) thetaiotaomicron and the development of bacteria-specific igg and iga in serum and specific secretory iga (siga) in feces of the animals ... | 2000 | 11205373 |
| experimental chronic arthritis and granulomatous inflammation induced by bifidobacterium cell walls. | effects of cell walls (cws) from two almost identical strains of bifidobacterium adolescentis were studied in rats, using three different doses. a single i.p. injection of both cws triggered a long-lasting arthritis with cw degradation products present in the joint tissue. histologically, the arthritis was characterized by inflammatory cells, synovial hyperplasia, pannus formation and bone erosion, closely resembling human rheumatoid arthritis (ra). in addition, cws of the other strain induced a ... | 2001 | 11439164 |
| [microelements preparations obtained during processing of natural flint affect the physiology and biochemistry of bifidobacteria]. | microelement preparations obtained in the course of processing of flint powder stimulate the biological activity of bifidobacterium adolescentis 94 bim, grown on complex and synthetic nutritive media. the composition of the microelement preparations differed in the content of cations and anions. introduction of the preparations into the cultures of physiologically active or anabiotic forms of bifidobacteria changed the parameters of exponential growth: compared to controls, the cultures were cha ... | 2001 | 11443901 |
| molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal dna restriction analysis (ardra). | one hundred and six isolates of the genus bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal dna restriction analysis. two restriction endonucleases (sau3ai and bamhi) were chosen for aligning the 16s rrna sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. a rapid and accurate identification scheme was obtained ... | 2001 | 11451515 |
| adhesion of bifidobacteria to granular starch and its implications in probiotic technologies. | adhesion of 19 bifidobacterium strains to native maize, potato, oat, and barley starch granules was examined to investigate links between adhesion and substrate utilization and to determine if adhesion to starch could be exploited in probiotic food technologies. starch adhesion was not characteristic of all the bifidobacteria tested. adherent bacteria bound similarly to the different types of starch, and the binding capacity of the starch (number of bacteria per gram) correlated to the surface a ... | 2001 | 11472921 |
| implantation of bacteria from human pulpal necrosis and translocation from root canals in gnotobiotic mice. | the aim of this study was to determine whether microorganisms recovered from infected human root canals were able to survive and translocate to a local lymph node when experimentally inoculated into the root canal system of germ-free mice. the microorganisms isolated from two patients with pulpal necrosis were inoculated in two groups of experimental animals; group i (gemella morbillorum) and group ii (bifidobacterium adolescentis, fusobacterium nucleatum, and clostridium butyricum). g. morbillo ... | 2001 | 11592487 |
| enteric bifidobacteria: isolation from human infants and challenge studies in mice. | bifidobacteria from breast-fed infants, formula-fed infants, or premature babies fed by parenteral methods were isolated and identified. the persistence of these microorganisms in the gastrointestinal tract of mice, after oral administration, was studied to determine the optimal dose and frequency of translocation to the liver and spleen. the rate of isolation among infants varied between 19 and 82% depending on the origin of the samples, with the highest values seen in breast-fed babies. the pr ... | 2001 | 11766054 |
| the detection of bifidobacterium adolescentis by colony hybridization as an indicator of human faecal pollution. | to develop an improved method for the detection of bifidobacterium adolescentis as an indicator of human faecal pollution. | 2002 | 11872129 |
| identification, detection, and enumeration of human bifidobacterium species by pcr targeting the transaldolase gene. | methods that enabled the identification, detection, and enumeration of bifidobacterium species by pcr targeting the transaldolase gene were tested. bifidobacterial species isolated from the feces of human adults and babies were identified by pcr amplification of a 301-bp transaldolase gene sequence and comparison of the relative migrations of the dna fragments in denaturing gradient gel electrophoresis (dgge). two subtypes of bifidobacterium longum, five subtypes of bifidobacterium adolescentis, ... | 2002 | 11976117 |
| changes in predominant bacterial populations in human faeces with age and with clostridium difficile infection. | the bacterial composition of human faeces can vary greatly with factors such as age and disease, although relatively few studies have monitored these events, particularly at species level. in this investigation, bacteria were isolated from faecal samples from healthy young adults and elderly subjects, and elderly patients with clostridium difficile-associated diarrhoea (cdad). the organisms were identified to species level on the basis of their cellular fatty acid profiles with the midi system. ... | 2002 | 11990498 |
| evaluation of n-acetylchitooligosaccharides as the main carbon sources for the growth of intestinal bacteria. | n-acetylchitooligosaccharides ((glcnac)(n)) with different degrees of polymerization (n=1-6) were prepared as the main carbon sources in media for evaluating the growth of nine intestinal bacteria. a chitohydrolysate was prepared by hydrolyzing shrimp-shell chitin using hcl. after purification, the purity of each (glcnac)(1-6) was >86%. the growth of intestinal bacteria was carried out in a basal medium (bm) containing 0.2% (w/v) of each sugar or glucose as the main carbon source and was evaluat ... | 2002 | 12007653 |
| [characterization of the cell-bound polysaccharides of bifidobacterium adolescentis 94 bim]. | the cell-bound polysaccharides (pss) of bifidobacterium adolescentis 94 bim were stripped from the cell surface with 2% sodium dodecyl sulfate (sds), 1.5% cetavlon, and 1% triton x-100 and purified by precipitation with 5 volumes of ethanol. according to the extraction conditions used, the polysaccharide preparations were designated as ps-sds-6 degrees c, ps-sds-100 degrees c, ps-cet, and ps-trit. the gel-permeation chromatography of the first two preparations with the use of a bio-gel p-10 colu ... | 2002 | 12024820 |
| [bifidobacterium adolescentis suppresses the humoral immune response to an autochthonous intestinal bacterium--experiments with gnotobiotic rats]. | on the basis of association-experiments with gnotobiotic rats, we described the immunogenicity of two selected bacterial species (bifidobacterium adolescentis and bacteroides thetaiotaomicron). b. adolescentis is a gram-positive lactic acid producing bacterium, strains of which are claimed to have probiotic properties. b. thetaiotaomicron is a gram-negative rod, autochthonous to the human as well as to the rats' intestinal tract. colonization of the gut was monitored by determination of bacteria ... | 2002 | 12058590 |
| design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples. | an oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ... | 2002 | 12167534 |
| identification of the source of faecal pollution in contaminated rivers. | identifying the source of faecal pollution is important to enable appropriate management of faecal pollution of water. four independent assays for faecal source discrimination have been developed and implemented in our lab. these assays detect fluorescent whitening agents, faecal sterols, bifidobacterium adolescentis and rhodococcus coprophilus. the combination of these indicators is able to identify human derived faecal pollution in rivers containing inputs from septic tanks, municipal oxidatio ... | 2002 | 12227609 |
| multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress. | using a flow cytometry-based approach, we assessed the viability of bifidobacterium lactis dsm 10140 and bifidobacterium adolescentis dsm 20083 during exposure to bile salt stress. carboxyfluorescein diacetate (cfda), propidium iodide (pi), and oxonol [dibac4(3)] were used to monitor esterase activity, membrane integrity, and membrane potential, respectively, as indicators of bacterial viability. single staining with these probes rapidly and noticeably reflected the behavior of the two strains d ... | 2002 | 12406706 |
| innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. | the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ... | 2002 | 12438343 |
| cytokine production in response to endodontic infection in germ-free mice. | this study evaluated the cytokine profiles (type 1 or type 2) that are triggered by and modulate endodontic periapical infections in the root canal system of germ-free mice. microorganisms isolated from two patients with pulpal necrosis were inoculated into two groups of experimental animals: group i (gemella morbillorum) and group ii (bifidobacterium adolescentis, fusobacterium nucleatum and clostridium butyricum). in vitro, g. morbillorum induced type 1 cytokine synthesis, while the modulation ... | 2002 | 12485325 |
| stimulation of the secretion of pro-inflammatory cytokines by bifidobacterium strains. | to characterize the ability of bifidobacteria to affect the production of macrophage-derived cytokines, a murine macrophage-like cell line, j774.1, was cultured in the presence of 27 strains of heat-inactivated bifidobacteria. bifidobacterium adolescentis and b. longum, known as adult-type bifidobacteria, induced significantly more pro-inflammatory cytokine secretion, il-12 and tnf-alpha, by j774.1 cells, than did the infant-type bifidobacteria, b. bifidum, b. breve, and b. infantis (p<0.01). in ... | 2002 | 12516776 |
| bifidobacterium adolescentis as a delivery system of endostatin for cancer gene therapy: selective inhibitor of angiogenesis and hypoxic tumor growth. | in order to overcome difficulties that hampered widespread application of antiangiogenesis in cancer therapy, a highly specific delivery system may be engaged in vivo to deliver and express antiangiogenic genes. we selected a strain of bifidobacterium adolescentis (b. adolescentis) as the delivery system to transport endostatin gene to solid tumors. b. adolescentis with endostatin gene were injected into tumor-bearing mice through the tail vein. after the mice were sacrificed, the tumor and some ... | 2003 | 12536198 |
| [amplification of bacterial heat shock protein 60 gene using inverse pcr method]. | a method is presented for rapid in vitro amplification of dna sequences of bacterial hsp60 gene. in our previous work, using degenerate oligonucleotide primers for conserved regions of hsp60 gene, a 600 bp fragment was amplified by pcr, cloned and sequenced. an inverse pcr method, with the primers oriented in the reversed direction of the usual orientation, is used to amplify the dna sequences that flank the 600 bp known region. the feasibility of this method is shown by amplifying the complete ... | 2002 | 12557349 |
| cloning and characterization of two alpha-glucosidases from bifidobacterium adolescentis dsm20083. | two alpha-glucosidase encoding genes (agla and aglb) from bifidobacterium adolescentis dsm 20083 were isolated and characterized. both alpha-glucosidases belong to family 13 of the glycosyl hydrolases. recombinant agla (ec 3.2.1.10) and aglb (ec 3.2.1.20), expressed in escherichia coli, showed high hydrolytic activity towards isomaltose and pnp-alpha-glucoside. the k(m) for pnp-alpha-glucoside was 1.05 and 0.47 mm and the v(max) was 228 and 113 u mg(-1) for agla and aglb, respectively. using pnp ... | 2003 | 12658515 |
| intestinal mucosa-associated bacteria modulate rat mast cell reactivity. | it is well known that in some conditions bacteria of physiological flora of gastrointestinal tract may become pathogenic. each complaint which causes injury of gastrointestinal wall integrity permits bacteria to penetrate the tissues and affect the tissue cells. since mast cells represent one of very important and numerous cellular elements of the gastrointestinal tract walls, bacteria can exert the effects on them. therefore, the aim of our study was to examine the influence of four strains of ... | 1999 | 12793960 |
| growth-inhibiting effects of seco-tanapartholides identified in artemisia princeps var. orientalis whole plant on human intestinal bacteria. | the present work aimed at isolating antibacterial constituents from the whole plant of artemisia princeps var. orientalis active towards nine human intestinal bacteria. | 2003 | 12807448 |
| interaction of bifidobacteria with the gut and their influence in the immune function. | bifidobacteria are predominant in the lumen of the large intestine and confer various health benefits on the host. they are also used in the preparation of new fermented milks (bioyogurts) or added to conventional yogurt to generate probiotic effects. the colonization of the gut by bacteria tends to be host specific due partly to the way in which bacteria adhere to the intestinal wall. using a homologous strain of bifidobacterium animalis in an experimental mouse model, we analyzed by immunofluo ... | 2003 | 12847910 |
| selective plating underestimates abundance and shows differential recovery of bifidobacterial species from human feces. | the aim of the present work was to compare the efficacies and levels of selectivity of different culture-dependent and -independent methods for analyzing bifidobacteria in human stool samples. the three different culture media used here significantly differed from each other, particularly with regard to the recovery of bifidobacterium adolescentis. bifidobacterium medium failed to recover b. adolescentis; beerens medium recovered some b. adolescentis organisms (17% of total bifidobacteria), wher ... | 2003 | 12957972 |
| genus- and species-specific pcr primers for the detection and identification of bifidobacteria. | 16srdna-targeted genus- and species-specific pcr primers have been developed and used for the identification and detection of bifidobacteria. these primers cover all of the described species that inhabit the human gut, or occur in dairy products. identification of cultured bifidobacteria using pcr primer pairs is rapid and accurate, being based on nucleic acid sequences. detection of bifidobacteria can be achieved using dna extracted from human faeces as template in pcr reactions. we have found ... | 2003 | 14503690 |
| quantitative pcr with 16s rrna-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria. | a highly sensitive quantitative pcr detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time pcr with bifidobacterium genus- and species-specific primers. real-time pcr detection of serially diluted dna extracted from cultured bifidobacteria was linear for cell counts ranging from 10(6) to 10 cells per pcr assay. it was also found that the method was applicable to the detection of bifidobacterium in feces when it was p ... | 2004 | 14711639 |
| physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from bifidobacterium adolescentis dsm20083. | clones of a genomic library of bifidobacterium adolescentis were grown in minimal medium with sucrose as sole carbon source. an enzymatic fructose dehydrogenase assay was used to identify sucrose-degrading enzymes. plasmids were isolated from the positive colonies and sequence analysis revealed that two types of insert were present, which only differed with respect to their orientation in the plasmid. an open reading frame of 1,515 nucleotides with high homology for sucrose phosphorylases was de ... | 2004 | 14740189 |
| crystal structure of sucrose phosphorylase from bifidobacterium adolescentis. | around 80 enzymes are implicated in the generic starch and sucrose pathways. one of these enzymes is sucrose phosphorylase, which reversibly catalyzes the conversion of sucrose and orthophosphate to d-fructose and alpha-d-glucose 1-phosphate. here, we present the crystal structure of sucrose phosphorylase from bifidobacterium adolescentis (bisp) refined at 1.77 a resolution. it represents the first 3d structure of a sucrose phosphorylase and is the first structure of a phosphate-dependent enzyme ... | 2004 | 14756551 |
| impact of consumption of oligosaccharide-containing biscuits on the fecal microbiota of humans. | human subjects consumed biscuits containing either galacto-oligosaccharides or fructo-oligosaccharides in a double-blinded, crossover study. the impact of supplementing the diet with three biscuits per day on the fecal microbiota was evaluated by selective culture of particular bacterial groups, measurement of beta-galactosidase activity, and nucleic acid-based analytical methods (pcr-denaturing gradient gel electrophoresis [pcr-dgge] and fluorescent in situ hybridization). the composition of th ... | 2004 | 15066805 |
| pattern of cytokine responses to gram-positive and gram-negative commensal bacteria is profoundly changed when monocytes differentiate into dendritic cells. | the normal gastrointestinal bacterial flora is crucial for the maturation of acquired immunity via effects on antigen-presenting cells (apcs). here we investigated how two types of apcs, monocytes and dendritic cells (dcs), react to different bacterial strains typical of the commensal intestinal microflora. purified human monocytes and monocyte-derived dcs were stimulated with uv-inactivated gram-positive (lactobacillus plantarum and bifidobacterium adolescentis) and gram-negative (escherichia c ... | 2004 | 15102775 |
| commensal gram-negative bacteria prime human dendritic cells for enhanced il-23 and il-27 expression and enhanced th1 development. | dendritic cells (dc) are the main orchestrators of specific immune responses. depending on microbial information they encounter in peripheral tissues, they promote the development of th1, th2 or unpolarized th cell responses. in this study we have investigated the immunomodulatory effect of non-pathogenic intestinal gram-negative (escherichia coli, bacteroides vulgatus,veillonella parvula, pseudomonas aeruginosa) and gram-positive (bifidobacterium adolescentis, enterococcus faecalis, lactobacill ... | 2004 | 15114670 |
| competitive inhibition of adherence of enterotoxigenic escherichia coli, enteropathogenic escherichia coli and clostridium difficile to intestinal epithelial cell line lovo by purified adhesin of bifidobacterium adolescentis 1027. | to observe competitive inhibition of adherence of enterotoxigenic escherichia coli (etec), enteropathogenic escherichia coli (epec) and clostridium difficile (c. difficile) to intestinal epithelial cell line lovo by purified adhesin of bifidobacterium adolescentis 1027 (b. ado 1027). | 2004 | 15162538 |
| [growth and morphological characteristics of industrial strains of bifidobacterium and lactobacillus cultivated in hydrolysate-milk and hydrolysate-soybean media]. | growth features of industrial strains of bifidobacterium adolescentis mc-42, b. bifidum 1, b. longum b-379, lactobacillus acidophilus, l. delbrueckii subsp. bulgaricus 8-79 and l. plantarum 8pa3 cultivated in hydrolysate-milk or hydrolysate-soybean media (hmm and hsm respectively) were analysed comparatively. the bacterial cells were investigated morphometrically with atom strength microscopy. it was shown that hsm vs hmm provided a higher growth rate of the strains (shortened growth phases and ... | 2003 | 15176097 |