Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| conformational and molecular responses to ph variation of the purified membrane adenosine triphosphatase of micrococcus lysodeikticus. | a preparation of atpase from the membranes of micrococcus lysodeikticus, solubilized and more than 95% pure, showed two main bands in analytical polyacrylamide gel electrophoresis. they did not correspond to isoenzymes because one band could be converted into the other by exposure to a mildly alkaline ph value. the conversion was paralleled by changes in molecular weight, circular dichroism and catalytic properties. denaturation by ph at 25 degrees c was followed by means of circular dichroism, ... | 1975 | 91 |
| membrane-reversible h+-atpase from micrococcus lysodeikticus. | treatment of phosphorylating fragments of bacterial membrane from micrococcus lysodeikticus with trypsin leads to increase atpase activity. as a result of this treatment, the membrane fragments acquire the ability to transform the atp energy into transmembrane difference in potential. dithiothreitol has a similar effect to that of trypsin on the membrane fragments from m. lysodeikticus. dicyclohexylcarbodimide inhibits atpase of the membrane fragments of m. lysodeikticus, and also the atpase-rea ... | 1976 | 4306 |
| exo-beta-n-acetylmuramidase--a novel hexosaminidase. production by bacillus subtilis b, purification and characterization. | exo-beta-n-acetylmuramidase, or beta-2-acetamido-3-o-(d-1-carboxyethyl)-2-deoxy-d-glucoside acetamidodeoxyglucohydrolase, is produced by bacillus subtilis b, growing in a succinate/peptone/salts medium, at the end of exponential growth and occurs partly in the medium and partly bound to the cells. a lysozyme digest of micrococcus lysodeikticus cell walls, o-2-acetamido-2-deoxy-beta-d-glucopyranosyl-(1 leads to 4)-2-acetamido-3-o-(d-1-carboxyethyl)-2-deoxy-d-glucose and o-2-acetamide-3-o-(d-1-car ... | 1976 | 6281 |
| isolation and partial characterization of the two major subunits of the bf1 factor (atpase) from micrococcus lysodeikticus and evidence for their glycoprotein nature. | 1976 | 6334 | |
| purification of several bacteriolytic enzymes by affinity chromatography on lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose. | using lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose, several bacteriolytic enzymes were purified from crude preparations of animal and microbial origin. quail egg-white, human milk and salivary lysozymes ec 3.2.1.17 were adsorbed onto the adsorbent at ph 5-7 and eluted with 2m nacl at ph 10. by means of these treatments, lysozymes were purified 20-250 fold with activity recoveries of 60-80%, and the quail lysozyme thus purified was shown to be discelectrophoretica ... | 1975 | 6436 |
| a transition state analog of lysozyme catalysis prepared from the bacterial cell wall tetrasaccharide. | treatment of the cell wall tetrasaccharide glcnacbeta(1 leads to 4)-murnac-beta(1 leads to 4)-glcnac-beta(1 leads to 4)-murnac with alkali resulted in the formation of the unsaturated tetrasaccharide glcnac-beta(1 leads to 4)-murnac-beta(1 leads to 4)-glcnac-beta(1 leads to 4)-delta2,3-2-acetamido-2-deoxy-d-glucoseen. the same compound was also formed by transglycosylation upon incubation of the unmodified tetrasaccharide with the unsaturated disaccharide glcnac-beta(1 leads to 4)-delta2,3-2-ace ... | 1976 | 6476 |
| effects of polyamines on the degradation of ribonucleic acids by polynucleotide phosphorylase of micrococcus luteus. | the effects of polyamines on the breakdown of synthetic polynucleotides poly(a), poly(c), and poly(u) by polynucleotide phosphorylase polyribonucleotide: orthophosphate nucleotidyltransferase, ec 2.7.7.8 from micrococcus luteus have been studied. although the breakdown of all the synthetic polynucleotides tested was stimulated by polyamines, the degree of stimulation by polyamines was in the order poly(c) greater than poly(a) greater than poly(u) at ph 7.5. however, the difference in degree of s ... | 1977 | 14945 |
| phosphate transport in micrococcus lysodeikticus. | phosphate accumulates in micrococcus lysodeikticus cells against a concentration gradient, by an energy-dependent process. the phosphate transport is derepressed during phosphate deprivation. the depression process is inhibited by chloramphenicol. the apparent km of phosphate transport is 4.3 micronm. the activation energy of the transport is 21 kcal per mol in the temperature range of 0-29degrees c, and 4.9 kcal per mol between 29 and 40degrees c. the rate of the transport increases in presenc ... | 1977 | 15596 |
| the enzymatic conversion of l-histidine to urocanic acid by whole cells of micrococcus luteus immobilized on carbodiimide activated carboxymethylcellulose. | whole cells of micrococcus luteus (formerly sarcina lutea atcc 9341) have been covalently linked to a carboxymethylcellulose support system, with the retention of histidine ammonia-lyase activity. the dependence of the rate of urocanic acid formation on ph, temperature, and added surfactant concentration was similar for the free and the immobilized cells. the immobilization procedure used is based on the carbodiimide activation of carboxymethylcellulose and has been optimized for the histidine a ... | 1977 | 15677 |
| tyramine oxidase activity in needle biopsy of normal livers and diseased livers. | tyramine oxidase and udp-glucuronyl transferase activities were determined in 52 diseased livers obtained by needle biopsy. 14 liver specimens were also subjected to acetyl coa carboxylase determination. tyramine oxidase level was elevated in livers with nonalcoholic fatty change or toxic hepatitis, and reduced in livers with fibrosis or chronic alcoholic injury. udp-glucuronyl transferase activity was reduced in livers with severe parenchymal damage or hyperbilirubinemia. acetyl coa carboxylase ... | 1999 | 15828 |
| clinical considerations in analgesic therapy. ii. narcotic analgesics. | 2000 | 16247 | |
| the testing of the antibiotic sensitivity of bacteria on an agar medium: the problem of a double zone of inhibition. | when the sensitivity of micrococcus luteus attc9341 to streptomycin, erythromycin, oleandomycin and spiramycin was tested by an agar diffusion method using antibiotic impregnated filter paper disks on unbuffered penassay seed agar two zones of inhibition were observed around the disks after an incubation period of 24 hours at 30 degrees c. the ph of the m. luteus seeded penassay seed agar was measured before and after 24 hours incubation at 30 degrees c and found to be 6.6 and 8.7, respectively. ... | 1977 | 16428 |
| alpha-adrenergic blocking action of fentanyl on the isolated aorta of the rabbit. | the contractile response of helically-cut strips of rabbit ascending aorta to transmural electrical stimulation was attenuated in a dose-dependent manner by treatment for 20 min with fentanyl, 10(-6) to 10(-5) m. fentanyl also shifted the dose-response curve of the contractile response of aorta to norepinephrine to the right. the response to transmural stimulation was more resistant to fentanyl than was the response to an equipotent dose of norepinephrine. the inhibitory effect of fentanyl was ... | 1977 | 16512 |
| effective treatment of diabetic retinopathy. | 2000 | 16836 | |
| diagnosis of equine crytorchidism. | 1977 | 17211 | |
| phosphonic analogues as antagonists of amino acid excitants. | 1977 | 17707 | |
| [nicotinamide coenzyme level in erythrocytes in children with cerebral paralysis]. | 1999 | 17846 | |
| [the problems of adverse effects to the liver of alpha-methyldopa]. | in 20 patients who were treated with alpha-methyldopa since at least one year the bilirubin content, the activities of aspartate and alanine-aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase as well as the retention of bromsulphalein were determined. in altogether 10 patients pathological findings of laboratory examinations were the result. the gamma-glutamyl transpeptidase activity proved to be particularly sensitive. by means of biopsy in 3 of these patients a fatty dege ... | 1999 | 17957 |
| are transmitter release statistics meaningful? | 2000 | 18678 | |
| bacteriorhodopsin-mediated photophosphorylation in halobacterium halobium. | the rate of halobacterial photophosphorylation was found to be a linear function of light intensity over a wide range (between 1 and 20 mw/cm2). at higher light intensities (above 25 mw/cm2) the atp-synthesizing system itself limits the maximal rate of photophosphorylation. the optimal external ph range for this type of photophosphorylation is between ph 6.2 and 7.2 external. the photophosphorylation rate is directly proportional to the bacteriorhodopsin content of the cells. the quantum require ... | 1977 | 19249 |
| influence of solute and hydrogen ion concentration on the water relations of some xerophilic fungi. | germination and growth of six xerophilic fungi, aspergillus flavus, aspergillus ochraceus, eurotium chevalieri, chrysosphorium fastidium, wallemia sebi and xeromyces bisporus were examined on media of a wide range of water activities (aw). the influence of three solutes, nacl, glycerol and a glucose/fructose mixture, was studied at ph 4-0 and ph 6-5 using a plate-slide technique. germination times and growth rates were affected by solute type, but the influence of ph was less marked. except for ... | 1977 | 19558 |
| analysis of the activity of micrococcus luteus endonucleases with respect to gamma-irradiated dna. | endonucleases from micrococcus luteus that induce single-strand breaks in gamma-irradiated dna have been separated chromatographycally into two groups. the first group involves two different enzymes: ap-endonuclease ii (mol. weight 30 000) and ap, uv-endonuclease i (mol. weight 15 000) that recognize alkali-labile lesions in gamma-irradiated dna and apurinic sites in dna heated at 70 degrees c, ph 6.08 ap-endonuclease ii in cooperation with dna polymerase from m. luteus and t4 phage-induced poly ... | 1977 | 20161 |
| studies on the heterotropic interaction of hemoglobin. ii. role of beta-146 and beta-2 histidines in the alkaline bohr effect. | 1998 | 21177 | |
| [betasympatholytic therapy of functional cardiovascular disorders]. | 1998 | 21464 | |
| monoamine metabolite levels in cerebrospinal fluid of psychotic women treated with melperone or thiothixene. | psychotic women with schizophrenic symptoms were treated with melperone 100 mg x 3 (n = 29) or thiothixene 10 mg x 3 (n = 34) using a double-blind procedure. before and during treatment, levels of hva, mopeg, and 5-hiaa, the major metabolites of da, ne, and 5-ht, were determined in lumbar cerebrospinal fluid by a mass fragmentographic technique. both treatments resulted in an elevation of the hva levels after 2 weeks, thiothixene having a more marked effect. the effect of thiothixene but not of ... | 1977 | 21644 |
| relationship between intramitochondrial citrate and the activity of carbamoyl-phosphate synthase (ammonia). | the possibility of control of the activity of carbamoyl-phosphate synthase (ammonia) (ec 2.7.2.5) in rat-liver mitochondria by variation in the intramitochondrial free mg2+ concentration has been investigated. carbamoyl-phosphate synthase activity was measured by coupling the formation of carbamoylphosphate to the synthesis of citrulline in a reaction mixture containing ammonia, bicarbonate, a source of atp, and ornithine. the synthesis of citrulline was inhibited by lowering the concentration o ... | 1977 | 21702 |
| the effect of ionophores on phosphate and arsenate transport in micrococcus lysodeikticus. | 1977 | 21813 | |
| in vivo oxidation of reduced nicotinamide-adenine dinucleotide phosphate by paraquat and diquat in rat lung. | intravenous injection of rats with 156 mumol/kg of paraquat or 140 mumol/kg of diquat produced, within 60 min, a sharp drop in the ratios of nadph to nadp in lung. the effect persisted for a time period of at least 24 h. exposure to 100% oxygen enhanced the toxicity of both compounds without substantially amplifying changes in the nadph/nadp ratio. lungs retained the capability to synthesize adenine nucleotides de novo. electron microscopic studies showed that both paraquat and diquat damage typ ... | 1977 | 22404 |
| mutagenic and recombinogenic action of pesticides in aspergillus nidulans. | thirteen pesticides, aminotriazole, benomyl, captafol, captan, dalapon-na, dichlorvos, dinobuton, dodine, ioxynil, mecoprop, neburon, picloram and tordon were tested for ability to induce (1) point mutations to 8-azaguanine resistance, (2) mitotic crossing-over, and (3) mitotic non-disjunction and haploidization in aspergillus nidulans. tests were performed at three different phs, i.e. 4.5, 7, 8.2. three of the pesticides, captan , captafol and dichlorvos induced point mutations; dichlorvos also ... | 1977 | 22812 |
| [advances in the treatment of hypertension]. | 1997 | 23521 | |
| methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase from porcine liver. interaction between the dehydrogenase and cyclohydrolase activities of the multifunctional enzyme. | methylenetetrahydrofolate dehydrogenase (5,10-methylenetetrahydrofolate: nadp+ oxidoreductase, ec 1.5.1.5) one of the activities of a trifunctional folate-dependent enzyme from porcine liver, uses an ordered kinetic mechannism as determined from initial velocity, product inhibition and dead-end inhibition studies. the final product released from the dehydrogenase is methenyltetrahydrofolate. however, from the time course of appearance of products it is observed that the methenyltetrahydrofolate, ... | 1978 | 23838 |
| detection and prevalence of pneumococci with increased resistance to penicillin. | susceptibility to penicillin was determined for 6000 strains of pneumococci isolated during 1974--76 from patients in alberta and the adjacent region of the northwest territories. strains were considered to be relatively resistant if the minimum inhibitory concentration (mic) of penicillin was 0.16 microgram (0.26 u)/ml or more, which is eight or more times greater than the mic for fully susceptible strains. resistance was detected in 143 strains (2.4%) isolated from 122 patients and belonging t ... | 1977 | 23894 |
| cardiovascular responses to nitrous oxide during light, moderate, and deep halothane anesthesia in man. | 1978 | 24374 | |
| active groups of extracellular endo-d-galacturonanase of aspergillus niger derived from ph effect on kinetic data. | in an attempt to characterize the groups essential for the catalytic action extracellular endo-d-galacturonanase of aspergillus niger (poly (1,4-alpha-d-galacturonide) glycanohydrolase, ec 3.2.1.15) the behaviour of the kinetic parameters as a function of ph was examined. the dependence of kcat and kcat/km on ph suggests that two dissociable groups are involved, for which the pk values of about 3.0 and 5.0 in the free enzyme and 3.06 and 5.72 in the catalytic complex were found at 30 degrees c. ... | 1978 | 24473 |
| purification and characterization of lysozyme produced by streptomyces erythraeus. | a species of lysozyme (se lysozyme) was purified from culture filtrate of streptomyces erythraeus. the enzyme has a molecular weight of 18,500 as determined by ultracentrifugation. its isoelectric point is 9.5, and it shows optimal activity at ph 4.0 with an optimal ionic strength of 0.1. investigation of the substrate specificity showed se lysozyme to be an n-acetyl-muramidase. the simplest product in the digest of cell walls of micrococcus lysodeikticus was identified as a disaccharide, glcnac ... | 1978 | 25274 |
| biochemical aspects of cardiac muscle differentiation. | experiments were designed to determine whether dna synthesis ceases in terminally differentiating cardiac muscle of the rat because the activity of the putative replicative dna polymerase (dna polymerase alpha) is lost or whether the activity of this enzyme is lost because dna synthesis ceases. dna-template availability and 3'-hydroxyl termini in nuclei and chromatin, isolated from cardiac muscle at various times during the developmental period in which dna synthesis and the activity of dna poly ... | 1978 | 26332 |
| [pharmacological regulation of lactation]. | 2000 | 26623 | |
| cell wall autolysis in log phase cells of micrococcus lysodeikticus (luteus). | log phase cells of micrococcus lysodeikticus (luteus) ifo 3333 autolyzed when incubated at 37 c in 0.01 m sodium-phosphate buffer ph 7.5. the enzyme involved in the autolysis was recovered mainly in an aqueous phase from cytoplasmic membranes and cytoplasmic materials treated with n-butanol, and proved to be an n-acetylmuramyl-l-alanine amidase. the autolysis of log phase cells suspended in autolyzing buffer was depressed by the addition of trypsin to the buffer. | 1978 | 27703 |
| an experimental model for osteomyelitis in the mandible. | 1977 | 28338 | |
| supersaturation of urine with uric acid and urate: response to a uricosuric diuretic. | in order to assess the possibility that treatment with the uricosuric diuretic ticrynafen (tienilic acid) could lead to ua or msu crystalluria, the degree of urinary supersaturation with respect to these crystalloids was assessed in persons receiving the diuretic for 1 week, and the results were compared with similar data generated from the use of probenecid. initially, only probenecid significantly increased the degree of urinary suspersaturation with respect to msu, and after adjustment of uri ... | 1997 | 28373 |
| the fluorescence of fluorescamine-amino acids. | 2001 | 30402 | |
| [single daily dose of pindolol in the treatment of arterial hypertension]. | 2001 | 30958 | |
| hr 756, the syn isomer of a new methoxyimino cephalosporin with unusual antibacterial activity. | hr 756, the syn derivative of 7-[(2-(2-amino-4-thiazolyl)-2-methoxyimino)acetamido]cephalosporanic acid, is a new semisynthetic cephalosporin. it was 80 times more active than the anti derivative against beta-lactamase-producing strains of gram-negative bacteria. the range of inhibitory concentrations of hr 756 against gram-negative bacteria, including haemophilus influenzae, susceptible or resistant to penicillins and cephalosporins was from 0.01 to 0.1 mug/ml. this activity was consistently hi ... | 1978 | 31836 |
| purification and properties of rabbit alveolar macrophage lysozyme. | lysozyme was isolated from bacillus calmette-guerin-elicited rabbit alveolar macrophages by acid extraction and purified to homogeneity by a single-column procedure. yields of the purified enzyme averaged between 20 and 30 mg per rabbit, values far in excess of those obtained with previously published methods. rabbit lysozyme has a molecular weight of 14,300 and exhibits optimal lytic activity against micrococcus lysodeikticus at an ionic strength of 0.04, ph 6.5. our results indicate that lysoz ... | 1979 | 37167 |
| determination of virginiamycin in feeds. | virginiamycin was extracted from the feed by ethanol-ph 2.5 phosphate buffer (1 + 1). the ph during extraction was adjusted (when necessary) to between 4 and 5. sample dilutions and the standard dose response line were prepared to contain ethanol ph 6 phosphate buffer (2 + 8), and the test organism was sarcina lutea. three feeds (a poultry ration, a swine finishing ration, and a swine starter ration) showed virginiamycin recovery of 88.8--108.9% when standard solutions were added at concentratio ... | 1979 | 39060 |
| biochemical properties of penicillin amidohydrolase from micrococcus luteus. | some biochemical properties of whole-cell penicillin amidohydrolase from micrococcus luteus have been studied. this whole-cell enzyme showed its maximal activity at 36 degrees c at ph 7.5. it was found that the activation energy of this enzyme was 8.03 kcal (ca. 33.6 kj) per mol, and this amidohydrolase showed first-order decay at 36 degrees c. the penicillin amidohydrolase was deactivated rapidly at temperatures above 50 degrees c during storage or preincubation for 24 h. the michaelis constant ... | 1979 | 39505 |
| characterization of the autolytic enzymes of clostridium perfringens. | clostridium perfringens and isolated walls of this organism autolysed rapidly when incubated in buffer at ph 7.0 with the release of free-reducing groups but no n-terminal amino acids. the predominant autolytic enzyme was an endo-beta-n-acetylglucosaminidase, and an endo-beta-n-acetylmuramidase was also present. the autolytic enzymes could be solubilized by extraction of the organisms with 5 m-licl and would then subsequently bind to and rapidly lyse walls of micrococcus luteus and, more slowly, ... | 1979 | 44314 |
| isolation and characterization of homogenous rabbit antibodies to micrococcus lysodeikticus with specificity to the peptidoglycan and to the glucose-n-acetylaminomannuronic acid polymer. | the antibody response of rabbits to micrococcus lysodeikticus is characterized by the production of a high concentration of antibodies which manifest markedly reduced heterogenicity. the specificity of these antibodies was studied and it revealed that m. lysodeikticus contains 2 major antigens: both the glucose-n-acetyl-aminomannuronic acid polymer obtained by formamide extraction of the cell walls and peptidoglycan solubilized by ultrasonic treatment gave precipitin reactions with hyperimmune a ... | 1975 | 52238 |
| mode of inhibition of herpes simplex virus dna polymerase by phosphonoacetate. | phosphonoacetate is a highly specific inhibitor of herpes simplex virus-induced dna polymerase. sensitivity of herpesvirus type 1 or type 2 induced dna polymerase to the drug was similar. however, dna polymerases from other sources such as the host cells (wi-38), micrococcus luteus, and hepatitis b virus were highly resistant. in addition, escherichia coli rna polymerase and reverse transcriptase of rous sarcoma virus were also insensitive to the drug. enzyme kinetic studies showed that inhibiti ... | 1975 | 53071 |
| [change in lipid-protein interactions in the membranes of bacteria exposed to gramicidin s]. | effect of cyclopeptide antibiotic gramicidin s on some enzymes and physical state of isolated micrococcus lysodeikticus membranes is studied. malate and lactate dehydrogenases were monotonously inhibited under the increase of gramicidin s concentration, while the activity of nadh-dehydrogenase firstly decreased and then reversed to the initial level under further increase of gramicidin s concentration. the oxygen uptake under oxidation of nadh and malate with membranes almost completely inhibite ... | 1976 | 58672 |
| physical facilities of medical school libraries in the united states, 1966-1975: a statistical review. | this paper, based on information received from a questionnaire to which 107 of the present 114 medical school libraries in the u.s. responded, shows the vast growth and expansion of such libraries. the 86 libraries which have been built, expanded, under construction of planned during 1966-1975 represent the greatest expansion in the history of medical school libraries. this "decade of revolution" can be attributed to the evergrowing resources, primary users, and services discussed in this paper. ... | 2001 | 58690 |
| chalcosis in the human eye. a clinicopathologic study. | a clinicopathologic study of eight human eyes containing intraocular copper foreign bodies was undertaken. these foreign bodies, which had been retained in the eyes from three weeks to 22 years, were analyzed by atomic-absorption spectrophotometry and x-ray diffraction to determine their chemical composition. copper deposits in descemet membrane, vitreous, internal limiting membrane of the retina, and fibrous capsule around the foreign bodies could be demonstrated by rhodanine, rubeanic acid, an ... | 1976 | 60098 |
| the action of local anaesthetics on histamine release. | 1976 | 60105 | |
| immunochemical study of the peptidoglycan of gram-negative bacteria. | the specificity of antibodies directed against the peptidoglycan of gram-negative bacteria was studied. the peptidoglycans of proteus vulgaris, escherichia coli, moraxella glucidolytica, neisseria perflava, give identical precipitin reactions. by means of inhibition studies with various peptidoglycan subunits and synthetic peptides, it was shown that the antibodies are essentially directed against the peptide moiety of the peptidoglycan: l-ala-d-glu (l)-mesoa2pm-(l)-d-ala, that the peptide react ... | 1976 | 60233 |
| radioimmunoassay of neocarzinostatin, an antitumor protein. | antibodies directed toward the antitumor protein neocarzinostatin (ncs) have been produced in a rabbit by immunization with a highly purified ncs preparation. the antiserum was monospecific and reversed the antibacterial activity of ncs against sarcina lutea. it cross-reacted with chemically modified derivatives of ncs and mitomalcin but failed to cross-react with macromomycin. a radioimmunoassay procedure has been developed utilizing the antiserum and a biologically active 125i-labeled derivati ... | 1976 | 63321 |
| the immune response to micrococcus lysodeikticus. 1. evolution of the response in two litter mates. | 1976 | 64217 | |
| restriction of the anti-bovine serum albumin response in rabbits immunized with micrococcus lysodeikticus. | rabbits capable of producing antibodies of restricted heterogeneity in response to micrococcus lysodeikticus are equally capable of producing antibodies of restricted heterogeneity to bovine serum albumin. these antibodies are produced when animals are simultaneously injected with micrococcus and bsa and their specificity is restricted to a small number of epitopes. these results suggest that micrococcal vaccines can induce the restriction of heterogeneity in antibodies raised against totally un ... | 1977 | 71263 |
| comparative immunological study of catalases in the genus micrococcus. | double immunodiffusion tests were performed with crude extracts from various micrococcus species and antisera against catalase of micrococcus luteus ccm 169. cell-free extracts of m. lylae atcc 27566 exhibited good cross-reaction. cell-free extracts or catalase enriched preparations of m. varians reacted very weakly and no reaction has been found with preparation of m. kristinae, m. nishinomiyaensis, m. roseus and m. sedentarius. the quantitative microcomplement fixation assay also revealed a cl ... | 1977 | 71880 |
| immunological properties of membrane-bound adenosine triphosphatase: immunological identification of rutamycin-sensitive f0.f1atpase from micrococcus luteus atcc 4698 established by crossed immunoelectrophoresis. | (1) f0.f1atpase (ec 3.6.1.3) from micrococcus luteus atcc 4698 was solubilized from plasma membranes by the non-ionic detergent triton x-100 in the presence of 0.05 m mgcl2. (2) the antibiotics rutamycin, dio-9, quercetin, oligomycin, botrycidin, efrapeptin, leucinostatin, valinomycin, and venturicidin as well as n,n'-dicyclohexylcarbodiimide and dinitrophenol are potent inhibitors of f0.f1atpase activity.(3) f0.f1atpase activity is completely inhibited by anti-f1atpase antibodies. the inhibitio ... | 1978 | 77681 |
| non-specific tumour immunotherapy by micrococcus lysodeikticus and related polysaccharides [proceedings]. | 1977 | 79358 | |
| polypeptide antibiotic 26a from bacillus subtilis. ii. isolation and purification procedures. | both the analytical and preparative methods by which the preparations of 26a bydrochloride salt with a high antibacterial activity and 20--30% recovery have been obtained from the fermentation fluids of bacillus subtilis are presented. on an industrial scale the antibiotic can be yielded by absorption of bioactivity on amberlite cg-50i column and precipitation with picric acid of crude substance from active elutes as adduct which was divided on equilibrated cm--cellulose and finally purified by ... | 1978 | 81596 |
| arterial occlusion of pelvic bone tumors. | arterial occlusion of the internal iliac artery was successful in the relief of pain due to primary and secondary neoplasms of the bony pelvis in 8 of 9 patients. these included 3 giant cell tumors, 1 aneurysmal bone cyst, 1 recurrent chondrosarcoma, 3 metastatic renal cell carcinoma and 1 metastatic clear cell sarcoma. calcification of the margin of the lesion occurred in 3 of 4 primary neoplasms after infarction. the transcatheter arterial occlusion was accomplished utilizing gelfoam and stain ... | 1999 | 83905 |
| reactions of chicken antibodies with immunoglobulins of mouse serum and t cells. | 1978 | 83961 | |
| graft-vs-host reaction in skin: histological immunological and ultrastructural studies. | a case of graft-vs-host reaction after bone marrow transplantation is described. histopathologic and ultrastructural findings in involved skin showed necrotic keratinocytes and abnormal melanocytes with satellite lymphocytes. no deposits of immunoglobulins or complement were found. these data provide direct evidence that chronic graft-vs-host disease in humans may be related to cellular hypersensitivity rather than to serum factors. | 1999 | 84472 |
| conception in a phenylketonuric woman. | 1999 | 84971 | |
| [neuroradiological diagnosis of third and para-third ventricular tumors--part 4 tumors developing in the posterior portion of the third ventricle (author's transl)]. | 1999 | 85281 | |
| epidermal cytoplasmic antigens. | 1999 | 85677 | |
| [effect of injuries on the functional properties of hemoglobin]. | 1999 | 85752 | |
| glucose tolerance and occupational exposure to carbon disulphide. | 1999 | 85888 | |
| carrier detection in duchenne muscular dystrophy. | 2000 | 86132 | |
| antitumour effect of cimetidine. | 2000 | 86136 | |
| carcinoid syndrome with cardiac involvement: successful palliative cardiac surgery. | 2000 | 86496 | |
| the new government. | 2000 | 86723 | |
| uses and abuses of chlormethiazole. | 2000 | 86826 | |
| correction of fallot's tetralogy after palliative operations. | during the period 1960 to 1978, 98 patients underwent intracardiac repair of fallot's tetralogy after palliative operations. preoperative symptoms were cyanosis, dyspnea, increased fatigue with squatting and hypoxic spells. the hemoglobin concentration varied from 19 to 22 g/100 ml. at correction only 65 of 95 shunts were patent and needed surgical closure. seventeen early deaths occurred (19%), the main causes being cardiac failure and arrhythmia. one patient died 3 years after correction from ... | 2000 | 87399 |
| screening for congenital hypothyroidism: results of screening one million north american infants. | pilot programs for screening of newborn infants for congenital hypothyroidism began in north america in 1972. to date, the five oldest programs (quebec, pittsburgh, toronto, oregon regional, and new england regional) have screened 1,046,362 infants. a total of 277 infants with congenital hypothyroidism have been detected and seven have been missed, resulting in a total of 284 affected infants in the screened population and an overall incidence of one in 3,684 live births. of the affected infants ... | 2000 | 87512 |
| low-dose heparin in breast-cancer surgery. | 2000 | 87604 | |
| manipulation, osteopathy, and back pain. | 2001 | 87812 | |
| human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus. | we have shown [mesa-tejada, r., keydar, i., ramanarayanan, m., ohno, t., fenoglio, c. & spiegelman, s. (1978) proc. natl. acad. sci. usa 75, 1529--1533] that an antigen immunologically related to gp52, a 52,000-dalton glycoprotein of the mouse mammary tumor virus, can be identified in sections of human breast cancer by means of an indirect immunoperoxidase technique. the specificity of the reaction was established by absorption experiments which revealed that only purified gp52, or material cont ... | 1979 | 88056 |
| [use in histological technic of naphtol as-d chloroacetate for demonstration of granulocyte series elements]. | the authors report their experience of a histo-enzymatic method, using naphtol as-d chloroacetate as a substrate, for the specific demonstration of elements of the granulocyte series, in histological sections, after formol fixation and mounting in paraffin wax. this simple and reliable staining technique offers the possibility of very rapid diagnostic orientation in haematological conditions. | 2001 | 88197 |
| phenoxybenzamine for benign prostatic hypertrophy. | 2001 | 88294 | |
| electroimmunoassay of factor ix in patients with liver damage and vitamin k unresponsive coagulation disorder. | factor ix antigen and activity were assayed in a group of patients with liver disease and in a group of patients in coumarin therapy. in patients with liver disease there was a similar decrease in activity and antigen. on the other hand factor ix activity is decreased in coumarin treatment with factor ix antigen remaining normal. factor ix electrophoretic mobility in liver disease is normal. | 2001 | 88391 |
| passive transfer in diabetes mellitus. | 1979 | 88629 | |
| [soft ulcer: problems in diagnosis and therapy]. | 2001 | 88826 | |
| a new assay of estrogen receptor by thin-layer gel filtration. | 1979 | 88911 | |
| [compartmentation of gramicidin 5 in membranes of sensitive bacteria and protein-lipid interactions]. | gramicidin s is sorbed on the isolated membranes of granicidin-sensitive micrococcus lysodeikticus strain. the antibiotic inhibits the membrane malate dehydrogenase within the temperature range of 9--42 degrees c, i.e. under conditions of gel and liquid-crystalline lipid state; however its effect at 10 degrees c is 10 times as low as is observed at 42 degrees c. the inhibitory effect of gramicidin s on malate dehydrogenase can be eliminated and the antibiotic can be removed from the membrane by ... | 1979 | 88965 |
| [heterogeneity in organization of lipids in the bacterial membrane]. | the lipids of micrococcus lysodeikticus membranes were 50%-substituted by phosphatidyl choline using lipid-exchanging proteins isolated from rat liver. the incorporation of phosphatidyl choline into the membrane did not significantly change the malate dehydrogenase activity and the temperature dependence activity in the arrhenius plots for the enzyme. gramicidin s--modifier of membrane lipids--had similar effects both on the intact membranes and on the phosphatidyl-enriched membranes. a conclusi ... | 1979 | 88966 |
| the early effects of chemical carcinogens on adult rat hepatocytes in primary culture. ii. effects on unscheduled dna synthesis, cell division and alpha-fetoprotein production. | the effects of exposure of rat hepatocytes in primary maintenance culture to chemical carcinogens has been studied with respect cytotoxicity and alterations in mitotic index, unscheduled dna synthesis and alpha-fetoprotein (afp) production. all compounds tested produced cytotoxicity. increases in mitotic index and unscheduled dna synthesis and the production of afp were observed after treatment of the cells with the carcinogens but not after treatment with the non-carcinogenic isomers. these inc ... | 1979 | 89011 |
| radioimmunoassay of "pregnancy-specific"-beta 1-glycoprotein (sp1). | a radioimmunoassay for sp1 ("pregnancy-specific" beta 1-glycoprotein) is described in which a sensitivity of 0.1 microgram/l was achieved by use of an antiserum with ka approximately equal to 1.7 x 10(11) l/mol and delayed addition of a 125i-sp1 tracer (specific acty, 25 ci/g) that was stable for use in the assay for at least six to seven weeks. we also report an additional anti-sp1 serum, from an early bleeding, that displayed "apparent positive cooperativity" in the igg but not the igm fractio ... | 1999 | 89016 |
| a new and universal free/bound separation technique for the "centria" automated radioimmunoassay system. | a new separation procedure based on the double-antibody technique has been adapted to the centria system. this procedure is universally applicable and lends itself to easy adaptation to commercial ria kits in which liquid reagents are used. this second antibody is covalently linked to agarose (sepharose) and the lyophilized powder is subsequently tableted for easy use on the instrument. the technique was applied to radioimmunoassay for thyrotropin (thyroid stimulating hormone), alpha-fetoprotein ... | 1999 | 89017 |
| cyclic gmp system in epidermis: i. effect of ischemia. | when keratome-sliced pig epidermis was floated on hank's balanced salt solution, we observed a rapid decrease in the intracellular level of cyclic gmp. a portion of the lost cyclic gmp was detected in the incubation medium. when the epidermis was kept in air at room temperature, the cyclic gmp level also decreased rapidly but to a lesser degree. incubating the epidermal slice at 37 degrees c in hank's balanced salt solution with the addition of 3-isobutyl-1-methyl xanthine (ibmx) prevented the d ... | 1979 | 89173 |
| new screening test for colorectal cancer developed in canada. | 1999 | 89207 | |
| [blood serum cholesterol fractions in diseases of the liver and biliary tract]. | 1999 | 89227 | |
| screening for cervical cancer. | 1999 | 89296 | |
| histiocytic medullary reticulosis. | 1999 | 89321 | |
| aspirin and bleeding-time. | 1999 | 89323 | |
| wholemeal bread and satiety. | 1999 | 89381 | |
| treatment of kidney-allograft rejection with cyclosporin a. | 1999 | 89479 | |
| clean intermittent catheterisation. | 1999 | 89505 | |
| schizophrenia and degradation of endorphins in cerebrospinal fluid. | 1999 | 89549 |