Publications

luminescence and respiratory activities of photobacterium phosphoreum. competition for cellular reducing power.	changes in the in vivo luminescence, respiratory activities, contents of cytochromes, extractable luciferase and nad(p)h-fmn reductase during growth of the wild (bright) strain of photobacterium phosphoreum and its dim mutant were determined. the intensity of the in vivo luminescence per cell increased 10 times in the wild strain and 750 times in the dim strain during logarithmic growth, while the contents of luciferase and nad(p)h-fmn reductase remained almost constant. it is suggested that a c ...	1975	5397
mechanism of action of beta-blockers in hypertension.		2001	14643
gamma-d paraproteinemia - a report of 16 cases.	paraproteins of the gammad class are very rare, being found in only 0.3% to 3.0% of the cases of all paraproteinemias. they are difficult to detect and therefore sometimes not found or mis-diagnosed. in the 6500 cases of paraproteinemia we have diagnosed over the last eight years, 16 of them have been gammad. consistent with the reports of other gammad paraproteinemias most of these 16 cases were of type l (13), most patients were male (11), the average age (55), was younger than that of patient ...	1991	50964
retinol-binding protein in malnutrition.		1999	90984
semantic congruity and lexical marking in symbolic comparisons: an expectancy hypothesis.		1999	91082
tetralogy of fallot in adults.		1999	91440
male fertility after successful chemotherapy for lymphoblastic leukaemia.		2000	91850
isotachophoresis of csf proteins in gel tubes especially gammaglobulins. an analytical and preparative technique for high-separation of csf proteins.	an isotachophoretic method using polyacrylamide gel (pag-itp) in a simple disc electrophoretic equipment with plastic tubes containing the gels, was elaborated and especially designed for studying the gammaglobulins in csf and serum from control subjects and patients with neurological disorders, especially known or probable ms. the device and the itp system used, including leading and terminating electrolytes and spacer substances, dividing the gammaglobulins in a reproducible way, are described ...	2001	92548
mutant of b-tropic murine leukemia virus synthesizing an altered polymerase molecule.	a nonconditional mutant of b-tropic murine leukemia virus (mulv), defective in polymerase, has been isolated by cloning chronically infected cells. the cell clone containing the mutant produced virus particles which were noninfectious. however, superinfection of the cells by replication-competent xc-negative viruses resulted in the rescue of virus capable of forming plaques in a modified xc test, termed the "complementation plaque assay" (a. rein and r. h. bassin, j. virol. 28:656-660, 1978). an ...	1979	92571
nursing care study: breast reconstruction.		2001	92783
[vasodilator activity of co2 in newly formed subcutaneous gas pockets in rats (proceedings)].		1979	92930
[biochemical diagnosis of gangliosidosis gm1].		1998	98915
pyruvate dehydrogenase deficiency in spinocerebellar degenerations.	to study the incidence of abnormalities of the pyruvate (pdh) or ketoglutarate (kgdh) dehydrogenase complexes in patients with spinocerebellar degenerations, we measured the activities of pdh and kgdh in platelet-enriched preparations from the blood of 14 patients. low pdh was found in 6 of the 14 patients; low kgdh was found in 2 of the 6. pdh-normal and pdh-abnormal patients could not be distinguished by clinical criteria. these results extend previous studies, which suggested abnormalities of ...	1998	106330
[methodical principles of tumor therapy using fast neutrons and instrumental as well as constructional requirements for the realization of isocentric neutron therapy using neutron-generator and cyclotron devices].		1998	106494
the cellulolytic enzymes of botryodiplodia theobromae pat. separation and characterization of cellulases and beta-glucosidases.	1. filtrates from cultures of different ages of botryodiplodia theobromae pat. were fractionated by gel filtration, ion-exchange chromatography and polyacrylamide-gel electrophoresis. 2. five cellulases (c1, c2, c3, c4 and c5) were found, and their molecular weights, estimated by gel filtration, were 46000-48000 (c1), 30000-35000 (c2), 15000-18000 (c3), 10000-11000 (c4) and 4800-5500 (c5). 3. cellulase c5 was absent from old culture filtrates. 4. cellulase c1 had little or no activity on cm-cell ...	1979	106849
[effectiveness of a preventive mass examination for cardiac risk factors (author's transl)].	18 months after a mass examination for coronary heart disease risk factors in employees of a large industrial firm, 75% of the subjects examined at that time were re-interviewed and measurements of blood pressure and weight repeated. despite an altogether inadequate longterm control of the risk factors detected, a part of the subjects examined had drawn consequences regarding their way of life by reducing weight, changing smoking habits and increasing physical activity, thus proving the effectiv ...	1998	107409
chemical structure of the cell wall of mycobacterium tuberculosis var. bovis, strain bcg.	bcg cell walls contain approximately 30% free lipids like other mycobacterial cell walls. the insoluble skeleton of the cell wall is made up of two covalently linked polymers, a peptidoglycan and an arabinogalactan mycolate, with which are associated non peptidoglycan amino acids and a glucan. we present data on two structural features: 1. the "non peptidoglycan" amino acids; they form two kinds of compounds: peptide chains which can be solubilized by proteolytic enzymes and a trypsin-chymotryps ...	1975	126548
proton translocation in cytochrome-deficient mutants of escherichia coli.	cytochrome-deficient cells of a strain of escherichia coli lacking 5-amino-levulinate synthetase have been used to study proton translocation associated with the reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase region of the electron transport chain. menadione was used as electron acceptor, and mannitol was used as the substrate for the generation of intracellular nadh. the effects of iron deficiency on nadh- and d-lactate-menadione reductase activities were studied in iron-deficie ...	1979	154508
[relation between endocrinology and psychiatry. some recent developments].	relations between endocrinology and psychiatry are developing in two main directions: behavioural effects of hormones. after the effects of peripheral hormones, recent works are concerned with a.c.t.h. and antidiuretic hormone (which interfere with memory process) and with hypothalamic peptides (which might have psychotropic properties, independant of their endocrine regulation function). -correlations between psychiatric syndroms and endocrine disorders. in some mentally depressed patients, dis ...	2003	165466
[hiatal hernia. cancer of the cardia].		1989	209553
surgical management of islet-cell adenoma in infancy.	persistent neonatal hypoglycemia is a potentially serious condition which should be recognized promptly, investigated thoroughly, and treated expeditiously. islet-cell adenoma causing hypoglycemia in infancy is very unusual. only 23 cases have been reported in the literature. this report documents eight cases of our own and summarizes diagnostic methods, proper medical preparation, and fundamental surgical management. prompt surgical intervention is emphasized, as this will relieve hypoglycemia ...	1988	211657
temperature-sensitive mutants of herpes simplex virus type 1 defective in transcriptional and post-transcriptional functions required for viral dna synthesis.		1978	214940
[hemodialytic treatment in very small children].		2003	262280
biology of the dental pulp.		1980	291327
osteosarcoma.		1980	291463
easy recording of extracellular action potentials.		2003	309048
immune response to a mammary adenocarcinoma. vi. blocking of tumor-specific cell-mediated cytotoxicity by antibodies to the cytotoxic lymphocyte.		1978	309904
winder l porter, m.d..		2004	321272
homogeneous immunoglobulins in the serum of irradiated and bone marrow reconstituted mice: the role of thymus and spleen.	the influence of thymectomy and splenectomy on the frequency and class distribution of homogeneous immunoglobulins (h-ig) in serum was studied in lethally irradiated (dba/2 x c57bl/rij)f1 mice reconstituted with syngeneic bone marrow. during four follow-up periods in the first 9 months after transplantation, the sham-operated controls and splenectomized animals developed transient h-ig in an average frequency of 14.2 and 15.7% respectively. there were no marked differences in the incidence of h- ...	2004	387578
a simple finger tourniquet.		2004	444776
letpomeningeal aspergillosis causing internal carotid artery stenosis.		2004	444826
the terminal oxidase of photobacterium phosphoreum. a novel cytochrome.	the terminal oxidase of photobacterium phosphoreum has been purified to the electrophoretically homogeneous state and some of its properties have been studied. the enzyme catalyses oxidation of ascorbate in the presence of phenazine methosulphate or n,n,n',n'-tetramethyl-p-phenylenediamine. the reaction is inhibited by cyanide. nitrite at comparatively high concentrations inhibits the enzyme, but the enzyme does not catalyse nitrite reduction with ascorbate plus the electron mediator as the elec ...	1979	465487
[results of 3 years resuscitation service in a general hospital].		2004	492374
[unexpected complications of behavior therapy of premature ejaculation].		2004	515257
thumb reconstruction after amputation at the interphalangeal joint by gradual lengthening of the proximal phalanx. a case report.	a case of thumb amputation at the interphalangeal joint and reconstruction by gradual lengthening of the proximal phalanx is described. as a result of the treatment the amputation stump was lengthened 2cms which means a 70 per cent increase of the original length of the proximal phalanx. the quality of sensibility of the stump has been preserved.	2004	520875
brenner's tumour of the ovary (review of literature and a report of four cases).		2005	546735
evidence for a fatty acid reductase catalyzing the synthesis of aldehydes for the bacterial bioluminescent reaction. resolution from luciferase and dependence on fatty acids.	the enzyme responsible for the stimulation by atp and nadph of light emission catalyzed by bacterial luciferase has been partially purified from extracts of the luminescent bacterium, photobacterium phosphoreum. the stimulatory activity was found to be stabilized by high concentrations of mercaptoethanol, permitting it to be separated from luciferase into an active and stable form and enabling further characterization of its functional properties. the activity of the enzyme was shown to be depen ...	1979	572825
luminescence and respiratory activities of photobacterium phosphoreum. ii. control by monovalent cations.	luminescent activity of spheroplasts of the cells of photobacterium phosphoreum was stimulated by rb+ and k+ and inhibited by na+ in the medium. opposite effects of these ions were observed on the rate of o2 consumption of the spheroplasts through the cytochrome and luciferase electron transfer systems. in vitro activities of nadh-fmn reductase and luciferase were only slightly stimulated by rb+, k+, and na+, while na+ exhibited significant activation of the nadh-oxidizing activity of the cells. ...	1977	599151
studies on luciferase from photobacterium phosphoreum. x. heat of formation of the intermediate in the bioluminescent reaction studied by stopped-flow calorimetry.	the heat production in the reaction of luciferase-fmnh2 complex with o2 in the absence of aldehyde was measured by stopped-flow calorimetry. deltah of the reaction, luciferase-fmnh2+ o2 leads to intermediate x1, is -1.3 x 10(2) kj.mol-1 and the calculated deltas for the reaction is -180 j.mol-1.k-1 at 20 degrees c. the heat production in the bioluminescent reaction was also measured in the presence of a saturating concentration of aldehyde, and it was estimated that 43 and 79% of the c10 and c13 ...	1978	659382
properties and kinetics of salt activation of a membrane-bound nadh dehydrogenase from a marine bacterium photobacterium phosphoreum.	a membrane-bound nadh dehydrogenase, solubilized and partially purified from a marine bacterium photobacterium phosphoreum, contains fad as the prosthetic group, and is specific for nadh. ferricyanide, various other redox dyes and cytochrome c can act as electron acceptors. the enzymatic activity when assayed with electron acceptors other than cytochrome c, is activated by monovalent cations (na+ and k+) and deactivated by high concentrations of monovalent anions (scn-, no3-, and cl-) but not by ...	1978	721793
studies on luciferase from photobacterium phosphoreum. xi. interaction of 8-substituted fmnh2 with luciferase.	the interaction of bacterial luciferase from photobacterium phosphoreum with reduced flavin was investigated using various 8-substituted fmnh2 analogs. flavins tested were fmnh2 and fmnh2 substituted at the 8 position with ho-, ch3o-, c2h5o-, cl-, br-, i-, h2n-, (ch3)hn-, and (ch3)2n. 8-ch30-, c2h5o-, cl-, and br-fmnh2 showed luminescent activity in the luciferase reaction with emission peaks at various wavelengths. 8-ho- and i-fmnh2 were competitive inhibitors toward fmnh2 in the luminescent re ...	1978	738995
studies on luciferase from photobacterium phosphoreum. ix. further studies on the spectroscopic characteristics of the enzyme-fmn intermediates.	the absorption and fluorometric changes of the reaction mixture of luciferase-fmnh2 complex with o2 were re-examined. rapid formation (k2(app) = 2.0 s-1 at [o2] = 120 micrometer) of an intermediate with a single absorption maximum at 380 nm within the range of 350-550 nm, and a weak fluorescence at 520 nm (less than or equal to 10% of that of fmn when excited at 380 nm) was observed. the absorption and fluorescence spectra and decay rate of the intermediate were estimated by simulation using an ...	1977	881410
studies on luciferase from photobacterium phosphoreum. viii. fmn-h2o2 initiated bioluminescence and the thermodynamics of the elementary steps of the luciferase reaction.	bacterial luciferase from photobacterium phosohoreum was found to produce bioluminescence on reaction with fmn and h2o2 in the presence of aldehyde. this luminescence is presumably produced by the same x1 intermediate as that found in fmnh2-o2 initiated luminescence. from the ratio of the light intensities of the fmn-h2o2 initiated reactions, we calculated the association constant of the reaction, luciferase+fmn+h2o2in equilibriumx1, and estimated its temperature dependence. from these results w ...	1976	950335
[resuscitation under the conditions of defensive war].		2005	1065165
[fluctuation in populations of t-cells and b-cells in the spleen of infected mice: application of cytotoxic test using anti-t-cell and anti-b-cell sera].		2005	1082516
[amyloid and the eye].		2005	1134731
[the luminescent bacteria test for clean water legislation].	luminescent bacteria (photobacterium phosphoreum) may be used, on the one hand, for classical toxicity tests based on the inhibition of respiration or growth and, on the other hand, their luminescence itself may become the test criterion. such a luminescence inhibition test is commonly called a luminescent bacteria test. it may be conducted with fresh bacteria or with conserved ones. the luminescence parameter is, just like the parameter oxygen consumption, a summative parameter for undisturbed ...	1992	1307824
[luminescence, growth and respiration as end points of the toxic effects on photobacterium phosphoreum--short report].		1992	1307830
gonadotrophin secretion in vitro by cells of a pituitary tumour from a patient with multiple endocrine neoplasia type i.	the aim was to investigate the hormone secretory products of a pituitary tumour from a patient with multiple endocrine neoplasia type i (men i) utilizing cell culture and immunoassay techniques.	2006	1352189
microtox ec50 values for drinking water by-products produced by ozonolysis.	the aim was to determine the microtox ec50 values of some aliphatic aldehydes and carboxylic acids of normal chain length with 1-14 carbon atoms since these compounds have been detected as ozonolysis by-products in drinking water. the aqueous ec50 values decreased with increasing chain length except for formaldehyde and for the c1-c7 acids. at chain lengths above c7, where methanolic saline solutions were utilized to promote solubility, the aldehydes were more toxic than their corresponding carb ...	1992	1376239
fluorescence study of the ligand stereospecificity for binding to lumazine protein.	6,7-dimethyllumazine derivatives, substituted at the 8-position with aldityls or monohydroxyalkyl groups, have been examined for their binding ability to lumazine apo-protein from two strains of photobacterium phosphoreum using fluorescence dynamics techniques. on the protein the lumazine has a nearly monoexponential decay of fluorescence with lifetime 13.8 ns (20 degrees c). in free solution the lifetime is 9.6 ns. the concentration of free and bound lumazine in an equilibrium mixture can be re ...	1992	1483455
investigations of phagocytosis concerning the immunological defence mechanism of mytilus edulis using a sublethal luminescent bacterial assay (photobacterium phosphoreum).	1. a simple method for the determination of phagocytosis activity using mussel hemocytes by measuring the bioluminescence is presented. 2. the immunological defence activity based on phagocytosis is measured and quantified by a luminescent bacterial assay with photobacterium phosphoreum. 3. the measuring system allows us to establish the stress of the immunological defence mechanism of organisms exposed to chemicals and polluted rivers or sewage. results with reference substances and the phagocy ...	1991	1677842
[effort toward theorization: a leading force in practice, education and research].		2006	1764937
crystallization and preliminary x-ray diffraction studies of a flavoprotein, fp390, from a luminescent bacterium, photobacterium phosphoreum.	a flavoprotein, fp390, obtained from a luminescent bacterium, photobacterium phosphoreum, in the purification of luciferase has been crystallized by the vapor-diffusion procedure. crystals obtained from polyethylene glycol 4000 solutions, whose x-ray photographs show powder diffraction patterns, were unsuitable for further crystallographic work. however, tetragonal crystals grown from potassium phosphate solution well diffracted x-rays beyond 3 a resolution. the space group of this crystal is p4 ...	1991	1783606
qsar studies of comparative toxicity in aquatic organisms.	this study investigated the relationships between the toxicities of common organic pollutants to the fathead minnow (pimephales promelas), to daphnia magna, to tetrahymena pyriformis and in the microtox test, which uses the luminescent bacterium photobacterium phosphoreum. the toxicity data were compiled from the literature, with the exception of 40 experimentally determined microtox data. encouraging correlations are seen, indicating significant relationships between fish toxicities and those t ...	1991	1815364
regression and cluster analysis of the acute toxicity of 267 chemicals to six species of biota and the octanol/water partition coefficient.	the acute toxicities of 267 compounds to six aquatic and one terrestrial species were investigated with correlation, principal component and cluster analysis techniques for relationships with each other and with the compounds' octanol/water partition coefficient. selection of the investigated chemicals was based on the availability of at least three of the following measured parameters: acute (24-h to 96-h) lethal concentrations (lc50) to the fish fathead minnow (pimephales promelas), the fish g ...	1991	1815369
evaluation of the genotoxic activity and acute toxicity of euphorbia splendens latex, a molluscicide for the control of schistosomiasis.	1. the latex of euphorbia splendens var. hislopii has a molluscicidal action at low concentration (ld90 less than 1.5 ppm or 1.5 micrograms/ml) against the vector snails of schistosomiasis. 2. in the present study, the latex in natura or after lyophilization was submitted to the ames test and the chromotest to evaluate genotoxicity, to the microtox system to determine acute toxicity, and to the chinese hamster ovary cell assay (cho) to measure cytotoxicity. 3. the latex had no mutagenic activity ...	1991	1823273
astrocytes: a possible primary site for colchicine-mediated neurotoxicity in the rat striatum.	ultrastructural changes in rat astrocytes including endfoot swelling and rupture of plasma membranes were seen as early as 1 h after injection of 6.0 micrograms colchicine (co) into the striatum. significant change in neuronal morphology was not seen by 24 h. astrocytes may therefore be primary targets for co-mediated damage, which then may have secondary neurotoxic consequences.	1991	1828783
cytotoxicity of a bis-gma dental composite before and after leaching in organic solvents.	cell culture techniques were used to determine the source of cytotoxic agents in a commercial bis-gma composite. the material was polymerized according to the manufacturer's directions and leachable components were removed by room temperature extraction in ethanol, chloroform, or toluene. the leachable components in the extracts were identified using infrared spectrographic analysis. thin layer chromatographic analysis was used to determine the number of constituents. these constituents were sep ...	2007	1828807
complexity and sequence identification of 24 rat v beta genes.	twenty-four tcr v beta genes were cloned by anchored pcr from the lewis rat strain and identified by nucleotide and amino acid sequence comparisons to known mouse v beta genes. rat v beta genes exist in 17 single-member and 3 multimember subfamilies and exhibit 86 to 94 and 72 to 92% nucleotide and amino acid sequence similarities, respectively, to their mouse counterparts. a single rat gene, designated v beta 20, having no previously known mouse counterpart was identified; a closely related gen ...	1991	1828824
development of species-specific hybridization probes for marine luminous bacteria by using in vitro dna amplification.	by using two highly conserved region of the luxa gene as primers, polymerase chain reaction amplification methods were used to prepare species-specific probes against the luciferase gene from four major groups of marine luminous bacteria. laboratory studies with test strains indicated that three of the four probes cross-reacted with themselves and with one or more of the other species at low stringencies but were specific for members of their own species at high stringencies. the fourth probe, g ...	1991	1854194
marine biosurfactants, iii. toxicity testing with marine microorganisms and comparison with synthetic surfactants.	eight synthetic and nine biogenetic surfactants were tested on their toxicity. because of their possible application as oil dispersants against oil slicks on sea, the test organisms used were marine microorganisms (mixed and pure cultures of bacteria, microalgae, and protozoa). bacterial growth was hardly effected or stimulated, whilst that of algae and flagellates was reduced. all substances tested were biodegraded in sea water. the bioluminescence of photobacter phosphoreum (microtox test) was ...	1991	1878108
the acute toxicity of pulse-dosed, para-substituted phenols to larval american flagfish (jordanella floridae): a comparison with toxicity to photoluminescent bacteria and predicted toxicity using log kow.	the acute toxicity of nine para-substituted phenols was determined using a pulse-exposure testing protocol and 8-day-old larval american flagfish (jordanella floridae). relative tolerance was assessed by determining the 2-h pulse exposure concentration causing 20 and 50% mortality (pe lc20 and pe lc50) over the subsequent 94 h. four bioassays were run for each phenol and yielded the following mean pe lc20 values (mg 1(-1)) in descending order of toxicity: p-aminophenol, 0.06; hydroquinone, 0.13; ...	1991	1891709
absorption of lactose from colon of newborn piglet.	piglets in three age groups (1-3, 9-11, and 16-25 days after birth) were used for in vivo colonic perfusions. studies compared an isosmolar (312 mosm) with a high osmolar (551 mosm) solution and two equimolar substrates (with hexose concentrations of 73.1 mm), lactose and glucose-galactose. from the isosmolar perfusates, lactose absorption was 0.43 +/- 0.04 in the 18-20 day olds and 1.04 +/- 0.2 mumol.cm-1.min-1 in the 1-3 day olds; absorption from the glucose-galactose solution was negligible i ...	1991	1907103
[primary projection of receptors of extrinsic ocular muscles: anatomical results].	recently, several electrophysical studies have shown that extraocular sensory afferents projected onto subcortical nuclei and cortical area as well. in a recent study of the projection fields of the primary afferent fibers from extraocular muscles, it has been shown that the intermediate layers of the cervical dorsal horn of the spinal cord was one of the recipient zone.	2007	1951928
the relationship between meteorological features and equine grass sickness (dysautonomia).	local weather patterns associated with 15 outbreaks of equine grass sickness in eastern scotland were studied. the majority showed a trend toward cooler drier weather associated with irregular ground frosts. this would not preclude the hypothesis that fungi might be connected with the aetiology of grass sickness.	1991	1959529
borrowed proteins in bacterial bioluminescence.	a library of photobacterium phosphoreum dna was screened in lambda 2001 for the lumazine protein gene, using two degenerate 17-mer oligonucleotide probes that were deduced from a partial protein primary sequence. the lumazine protein gene was localized to a 3.4-kilobase bamhi/ecori fragment in one clone. the fragment contained an open reading frame, encoding a 189-residue protein, that had a predicted amino acid sequence that concurred with the partial sequence determined for lumazine protein. c ...	1991	1996310
structure and properties of luciferase from photobacterium phosphoreum.	the nucleotide sequences of the luxa and luxb genes coding for the alpha and beta subunits, respectively, of luciferase from photobacterium phosphoreum have been determined. the predicted amino acid sequences of the alpha and beta subunits were shown to be significantly different from other bacterial luciferases with 62 to 88% identity with the alpha subunits and 47 to 71% identity with the beta subunits of other species. expression of the different luciferases appear to correlate with the numbe ...	1991	2018544
identification of the acyl transfer site of fatty acyl-protein synthetase from bioluminescent bacteria.	fatty acid activation, transfer, and reduction by the fatty acid reductase multienzyme complex from photobacterium phosphoreum to generate fatty aldehydes for the luminescence reaction is regulated by the interaction of the synthetase and reductase subunits of this complex. identification of the specific site involved in covalent transfer of the fatty acyl group between the sites of activation and reduction on the synthetase and reductase subunits, respectively, is a critical step in understandi ...	1991	2023262
improvement in anemia by recombinant human erythropoietin in patients with myelodysplastic syndrome and aplastic anemia.		1991	2040187
analysis of synchronous photon emissions from the bacterium photobacterium phosphoreum during colony formation from a single cell.	light emission from photobacterium phosphoreum was analysed during cell growth on an agar plate from a single cell to colony formation. temporal analysis of image intensified light was set so that a quadratic window covered a single cell. intensity of light emission from a single cell through colony formation showed an initial decrease, a prolonged lag phase, and then a rapid increase. these responses on an agar plate were similar to those from liquid cultures. the image analysis showed repeated ...	1991	2053463
further study on polyamine compositions in vibrionaceae.	it has previously been reported that norspermidine, one of the unusual polyamines, is present in vibrio species. to expand this observation, the cellular polyamine compositions of additional species and strains in the family vibrionaceae (vibrio, photobacterium, listonella, and shewanella) as well as aeromonas species and plesiomonas shigelloides, which have been proposed to be excluded from vibrionacea, were determined by using gas-liquid chromatography. some vibrio species previously reported ...	1991	2059921
electronic excitation transfer in the complex of lumazine protein with bacterial bioluminescence intermediates.	fluorescence dynamics measurements have been made on the bioluminescence reaction intermediates using photobacterium leiognathi, vibrio fischeri, and vibrio harveyi luciferases, both alone and in mixtures with photobacterium phosphoreum lumazine protein. each luciferase produces a "fluorescent transient" intermediate on reaction with the bioluminescence substrates, fmnh2, tetradecanal, and o2, and all have a fluorescence quantum yield about 0.3, with a predominant lifetime around 10 ns. the p. l ...	1991	2069948
a lux-specific myristoyl transferase in luminescent bacteria related to eukaryotic serine esterases.	the diversion of fatty acids from fatty acid biosynthesis into the luminescent system is catalyzed by a lux-specific acyltransferase that catalyzes the cleavage of fatty acyl-acyl carrier protein (acp). analysis of the substrate specificities for fatty acyl-acps of the transferases from divergent luminescent bacteria, photobacterium phosphoreum and vibrio harveyi, has demonstrated that myristoyl-acp is cleaved at the highest rate. inhibition by phenylmethanesulfonyl fluoride as well as resistanc ...	1991	2071574
intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study.	light and electron microscopy were used to analyze nine therapy-resistant and asymptomatic human periapical lesions, which were removed as block biopsies during surgical treatment of the affected teeth. the cases that required surgery represented about 10% of all of the cases which received endodontic treatment and root fillings during the period 1977 to 1984. these cases revealed periapical lesions when they were examined 4 to 10 yr after treatment. the biopsies were processed for correlated li ...	1990	2094761
loss of mhc class-i expression in cervical carcinomas.	the expression of mhc class-i antigens was analysed in 67 cervical carcinoma biopsies; 16% of the biopsies showed complete or heterogeneous loss of hla expression as judged by reactivity with antibodies recognizing monomorphic determinants of the class-i heavy chain bound to beta 2 microglobulin (beta 2m). in addition, other biopsies showed a loss in expression of particular allelic products: 23% for hla-a2; 17% for hla-a3; 23% for hla-bw4 and 19% for hla-bw6. three biopsies showed changes at 2 ...	1990	2174412
affinity purification of bacterial luciferase and nad(p)h:fmn oxidoreductases by fmn-sepharose for analytical applications.	a modified purification method for bacterial luciferases and nad(p)h:fmn oxidoreductases is described which uses fmn-sepharose alone or coupled to deae ion exchange chromatography for the simultaneous purification of luciferase and the various oxidoreductases from vibrio harveyi, a bright mutant of vibrio harveyi, vibrio fischeri, and photobacterium phosphoreum. this purification method is compared with deae-sepharose cl 6b fractionations from these organisms. both methods allow the separation o ...	1990	2220416
a soluble fatty acyl-acyl carrier protein synthetase from the bioluminescent bacterium vibrio harveyi.	an enzyme catalyzing the ligation of long chain fatty acids to bacterial acyl carrier protein (acp) has been detected and partially characterized in cell extracts of the bioluminescent bacterium vibrio harveyi. acyl-acp synthetase activity (optimal ph 7.5-8.0) required millimolar concentrations of atp and mg2+ and was slightly activated by ca2+, but was inhibited at high ionic strength and by triton x-100. acp from either escherichia coli (apparent km = 20 microm) or v. harveyi was used as a sub ...	1990	2223012
the lumazine protein gene in photobacterium phosphoreum is linked to the lux operon.		1990	2243804
[echocardiographic diagnosis of ischemic heart disease].		2007	2258676
bacterial luminescence: a new tool for investigating the effects of acoustic energy and cavitation.	an assay utilizing luminescent bacteria, photobacterium phosphoreum, was adapted to assess the antibacterial effects of acoustic energy. acoustic pressures up to 67 kpa in the 100- to 800-hz frequency range were applied to bacteria freely suspended in a liquid medium. bacterial luminescence decreased after sonication, thus showing sensitivity to the effects of acoustic energy. this decreased luminescence was linearly related to exposure duration, appeared independent of acoustic frequency in thi ...	1990	2283427
medical civil disobedience.		2008	2292675
13c and 15n nmr studies on the interaction between 6,7-dimethyl-8-ribityllumazine and lumazine protein.	the interaction between the prosthetic group 6,7-dimethyl-8-(1'-d-ribityl)lumazine and the lumazine apoproteins from two marine bioluminescent bacteria, one from a relatively thermophilic species, photobacterium leiognathi, and the other from a psychrophilic species, photobacterium phosphoreum, was studied by 13c and 15n nmr using various selectively enriched derivatives. it is shown that the electron distribution in the protein-bound 6,7-dimethyl-8-ribityllumazine differs from that of free 6,7- ...	1990	2331466
inhibition of bioluminescence in photobacterium phosphoreum by sulfamethizole and its stimulation by thymine.	in bioluminescent bacteria very few agents have been reported that can selectively inhibit the luminescence. in sensitivity tests with photobacterium phosphoreum, using 55 different antibiotics, it was found that sulfamethizole, an inhibitor of dihydropteroate synthetase and the formation of folic acid, inhibited bioluminescence more than growth. likewise, in mutants requiring thymine for growth, the luminescence per cell was much less in a medium low in thymine. in neither case could the decrea ...	1990	2372557
elongation of exogenous fatty acids by the bioluminescent bacterium vibrio harveyi.	bioluminescent bacteria require myristic acid (c14:0) to produce the myristaldehyde substrate of the light-emitting luciferase reaction. since both endogenous and exogenous c14:0 can be used for this purpose, the metabolism of exogenous fatty acids by luminescent bacteria has been investigated. both vibrio harveyi and vibrio fischeri incorporated label from [1-14c]myristic acid (c14:0) into phospholipid acyl chains as well as into co2. in contrast, photobacterium phosphoreum did not exhibit phos ...	1989	2492504
michigan doctor encourages children to plant trees, help environment.		2006	2586341
[specific properties of dna from fractions of isolated mouse synaptonemal complexes].		1989	2598773
[specific properties of dna from fractions of isolated mouse synaptonemal complexes].		1989	2598773
the annexin family of calcium-binding proteins. review article.	the annexins are a family of calcium-binding proteins. data from protein and cdna sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. the proteins are present within a wide range of tissues and cell types, with each cell type having all or a subset of the proteins. the proteins are localised on the inner surface of the plasma membrane ...	2009	2659190
evolution of a trna operon in gamma purple bacteria.	genomic dna from eubacteria belonging to the gamma-3 subdivision of purple bacteria, as classified by woese (c.r. woese, microbiol. rev. 51:221-271, 1987), were probed with the argt operon of escherichia coli encoding 5'-trna(arg)-trna(his)-trna(leu)-trna(pro)-3'. the homologous operon from vibrio harveyi was isolated and sequenced. comparison of the five available sequences of this trna cluster from members of the families enterobacteriaceae, aeromonadaceae, and vibrionaceae led to the conclusi ...	1989	2687235
dynamic fluorescence study of the interaction of lumazine protein with bacterial luciferases.	the equilibrium association of lumazine protein from photobacterium phosphoreum with luciferases from either p. phosphoreum or an aldehyde-requiring dark mutant of vibrio harveyi is measured from changes of the rotational correlation time which is derived from the decay of the lumazine ligand's fluorescence anisotropy. the rotational correlation time of lumazine protein is 23 ns (2 degrees c, 0.25 m pi) and is increased on addition of luciferase due to the formation of a higher molecular weight ...	1989	2720095
interaction between luciferases from various species of bioluminescent bacteria and the yellow fluorescent protein of vibrio fischeri strain y-1.	the interaction of the yellow fluorescent protein (yfp) from vibrio fischeri strain y-1 with luciferases from other bioluminescent bacterial strains have been studied. addition of purified yfp to a y-1 luciferase assay results in enhancement of the intensity of blue (484 nm) bioluminescence and a new peak in the emission spectrum at about 540 nm. the bimodal spectrum also resulted when the luciferase used in the reaction was isolated from the species v. fischeri atcc 7744, v. fischeri y-1, photo ...	1989	2742584
covalent reaction of cerulenin at the active site of acyl-coa reductase of photobacterium phosphoreum.	inhibition of bioluminescence in photobacterium phosphoreum by cerulenin has been demonstrated to be due to a specific inactivation of the acyl-coa reductase subunit of the fatty acid reductase complex required for synthesis of the aldehyde substrate for the luminescent reaction. in contrast, the activities of the other luminescence-related enzymes, acyl-protein synthetase, acyl-transferase, and luciferase, were unaffected by cerulenin. myristoyl-coa, but not nadph, protected the acyl-coa reduct ...	1989	2751874
alcohol and other predictors of cognitive recovery after severe head injury.	the research examined the effects of alcohol and other variables on cognitive outcome after severe head injury. alcohol consumption habitually and at the time of injury were strongly related, and both were related to age and educational level but not injury severity. covariance analysis to remove the effects of age and education showed a reduction in the main effects, so that only alcohol consumption at injury was a significant predictor of memory, but not other cognitive areas late after injury ...	2009	2758187
bioluminescence spectral and fluorescence dynamics study of the interaction of lumazine protein with the intermediates of bacterial luciferase bioluminescence.	the mechanism of the shifting of the bioluminescence spectrum from the reaction of bacterial luciferase by lumazine protein is investigated by methods of fluorescence dynamics. a metastable intermediate is produced on reaction of vibrio harveyi luciferase with fmnh2 and o2. it has an absorption maximum at 374 nm and a rotational correlation time (phi) derived from the decay of its fluorescence (maximum 500 nm) anisotropy of 90 ns (2 degrees c). lumazine protein from photobacterium phosphoreum ha ...	1989	2765486
time-resolved fluorescence spectroscopy of lumazine protein from photobacterium phosphoreum using synchrotron radiation.	time-resolved fluorescence on lumazine protein from photobacterium phosphoreum was performed with synchrotron radiation as a source of continuously tunable excitation. the experiments yielded structural and dynamic details from which two aspects became apparent. from fluorescence anisotropy decay monitoring of lumazine fluorescence with different excitation wavelengths, the average correlation times were shown to change, which must indicate the presence of anistropic motion of the protein. a sim ...	1989	2767000
counseling as part of primary care.		2009	2798275
counseling as part of primary care.		2009	2798275
[amino acid uptake in brain tumors using positron emission tomography as an indicator for evaluating metabolic activity and malignancy].	diagnosis and post-therapeutic follow-up of tumour patients necessitates morphological and particularly functional imaging methods. for the latter approach positron emission tomography has proven a valid tool for the measurement of perfusion, of energy consumption parameters such as oxygen extraction, glucose metabolism and amino acid uptake. however, neither perfusion nor energy consumption parameters have yielded unambiguous information on the clinical status of various tumours in respect of t ...	2010	2825284
transcription from a plant gene promoter in animal cells.	the promoter segment of a plant gene (maize alcohol dehydrogenase 1 (adh 1)) has been fused to two bacterial reporter genes, ecogpt (1) and neo (2), in psv2-derived vectors and introduced into cultured mammalian cells by dna transfection. the padh1-gpt plasmids transformed the recipient cells for resistance to mycophenolic acid plus xanthine (3) and the analogous padh1-neo plasmid transformed cells to g418 resistance (2). s1 analysis of transient transfections of cv1 cells with various derivativ ...	1985	2866489