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isolation of enterotoxigenic aeromonas from fish.this paper reports two different enterotoxigenic classes of the genus aeromonas isolated from fishes. strains of a. sobria were isolated from healthy fishes only wheras strains of a. hydrophila were isolated from both healthy and moribund fishes. aeromonas sobria and hydrophilia strains produced a cytotoxic factor and were highly proteolytic. seventy-five percent (75%) of the a. sobria strains produced only one type of hemolysis on 5% blood agar, but 92% of the a. hydrophilia produced two types ...197771190
pseudomembranous colitis. 197877373
prenatal methylmercury poisoning. clinical observations over five years.thirty-two infants prenatally exposed to methylmercury and their mothers were examined over a five-year period after the iraqi methylmercury epidemic. severity of poisoning in mothers was related to the peak mercury concentration in their hair and in the infants to the maximum concentration in maternal hair during pregnancy. in nine cases of cerebral palsy, methylmercury exposure occurred only during the last trimester. all infants except three (two were orphaned soon after birth and one was bot ...199984530
thyrotoxicosis during pregnancy.the diagnosis of thyrotoxicosis during pregnancy is difficult on clinical grounds only. the determination of free thyroid hormones or free thyroid hormone indices is important and is possibly supplemented by the trh stimulation test. thyrotoxicosis during pregnancy should always be treated actively. the authors recommend subtotal thyroidectomy if there are indications for surgical therapy (nodular goitre or large diffuse goitre). otherwise treatment with thyrostatic agents is used. after operati ...199984547
captopril in a hyponatraemic hypertensive: need for caution in initiating therapy. 199985145
in vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse transcriptase.infectious dna molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the rous sarcoma virus-associated reverse transcriptase in vitro. the optimal enzymatic conditions employed for infectious dna synthesis also facilitate maximum synthesis of genome length dna. analysis of the dna product synthesized by detergent-disrupted rous sarcoma virus under these conditions indicates that dna complementary to viral rna (minus-strand dna) is genome length in size, whereas dna ...197985719
dithranol or photochemotherapy for psoriasis? 200086005
two-week postnatal examination of infants. 200087641
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. 197988270
haemodilution and blood-flow. 199988643
[evaluation of blood-liquor barrier by protein gradients in congenital hydrocephalus in infancy]. 199988669
con-a-activated t cells secrete factors with polyclonal b-cell-activating properties.concanavalin a induced polyclonal antibody synthesis in normal spleen cells in vitro. optimal responses were obtained by con a concentrations lower than those optimal for induction of dna synthesis. t cells, but not macrophages, were necessary for the effect. spleen cells from nude mice were not activated, whereas cells from the lps non-responder stain c3h/hej were activated to polyclonal antibody synthesis by con a. supernatants from con a activated spleen cells could by themselves induce polyc ...200088758
[immunohistochemical study of the differentiation of the cephalic lobe of the adenohypophysis in chick embryos].it was shown by means of indirect immunofluorescence that acth appeared in the cephalic lobe of the chick embryo pituitary beginning from the 8th day of the development. a new tissue-specific antigen (a3) is revealed, which is localized in the cephalic lobe of adenohypophysis and becomes manifest at the 7th day of embryogenesis. quantitative analysis of acth and a3 localization in the cells of 11-day chick embryo adenohypophyses allows a conclusion that a3 is localized in corticotropic cells.200188969
control of v kappa expression in the mouse. i. unexpected expression of the v kappa allele, igk-pcb, in a somatic cell hybrid of akr (igk-pca) origin. 197989158
increase in leg blood-flow by normovolaemic haemodilution in intermittent claudication.normovolaemic haemodilution down to a packed-cell volume of 35% was done in 10 patients with stable intermittent claudication. for a mean reducation in the packed-cell volume of 21.5% there was a 170% increase in peak calf blood-flow and a 111% increase in haemoglobin delivery. 7 of the 10 patients reported an increased walking distance. normovolaemic haemodilution may be of use in the management of some patients with peripheral vascular disease.199989279
fetal antigens and prenatal diagnosis. 200089698
modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase inhibitors.it has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ...197989899
the association of carbohydrate with tubulin and in vitro assembled microtubules from bovine brain.phosphocellulose-purified tubulin (pc tubulin) was analyzed for neutral and amino sugar content, which was found to be 8.3 +/- 0.11 and 0.8 +/- 0.02 mol/mol dimer, respectively. a histochemical-electron-microscopic investigation was undertaken to attempt to localize carbohydrate associated with polymerized microtubules (mt). outer diameters of mt assembled in vitro from bovine brain mt protein (tubulin and microtubule associated proteins) were found to increase upon treatment with ruthenium red, ...197990041
characterization of in vitro proliferative responses of human lymphocytes to leishmanial antigens.leishmaniasis is an intracellular protozoal infection for which host defense is believed to depend on cellular immune mechanisms. the in vitro proliferative responses of lymphocytes from patients with leishmaniasis and from control subjects to leishmanial antigens were examined. only lymphocytes from patients responded to 1 microgram of leishmanial antigen/ml, whereas both patient and control lymphocytes responded to 10 micrograms/ml. the nonspecific responses were most likely not due to a mitog ...197990105
antigenic determinants in the post-mitochondrial lung fraction. 197990649
the role of eosinophil receptors in the non-genomic response to oestrogens in the uterus. 197990751
post-menopausal oestrogens protect against fractures of hip and distal radius. a case-control study.to assess the efficacy of oestrogens in preventing the common major limb fractures of postmenopausal women, retrospective case-control research was used for a new purpose--to explore beneficial rather than adverse effect of treatment. postmenopausal women admitted with fractures of the hip or distal radius and matched women admitted to the orthopaedic service for other reasons were surveyed. the "risk" of the non-oestrogen users, was estimated by inverting the customary odds ratio to calculate p ...200190802
prostacyclin overproduction in bartter's syndrome.urinary excretion of 6-keto-prostaglandin f1alpha and thromboxane b2, the major metabolites of prostacyclin and of thromboxane a2, respectively, was measured by specific radioimmunoassays in five female patients with bartter's syndrome and in five normal female controls. the patients with bartter's syndrome excreted about four times as much 6-keto-pgf1alpha as the controls; their excretion of thromboxane b2 was no different from that of the controls. these data suggest that overproduction of pro ...200190861
[unit for bleaching acrylamide gels]. 199991719
the search for indices. 200091795
[a comparison of the immunosuppressive effect of normal and purified alpha-globulin fraction of human plasma]. 197992373
antiviral agents: action and clinical use.the development of antiviral agents has been hindered by a variety of problems. there are fundamental biological differences between viruses and other infectious agents. viruses are strictly dependent on cellular metabolic processes and possess very limited intrinsic enzyme systems and building blocks which may serve as targets for drugs. antiviral drugs must also possess the ability to enter the host cell. viral replication consists of a series of events, each of which can be interfered with, l ...197992398
current concepts in ophthalmology. ocular infections. 197896339
diminished cellular immunity due to impaired nutrition in oesophageal carcinoma.the nutritional status of 15 negro patients suffering from unresectable carcinoma of the midthoracic oesophagus was evaluated before and after palliative pulsion intubation. all were shown to be in negative nitrogen balance and to have a compromised non-specific cellular immune response. correction of protein-calorie malnutrition and the achievement of positive nitrogen balance were associated with an increase in absolute lymphocyte and t lymphocyte numbers and a significant increase in lymphocy ...199996903
effect of dietary composition on fasting-induced changes in serum thyroid hormones and thyrotropin. 200097492
long term effects of myochrysine in articular cartilage.intra-articularly administered sodium aurothiomalate (myochrysine) produced aurosomes containing characteristic electron dense contents (indicating the presence of gold), in the chondrocytes of rabbit articular cartilage. at first the aurosomes were bounded by a membrane but later the electron dense contents were seen lying free in the cytoplasmic matrix. such deposits were detectable up to 14 months after injection of myochrysine but none were found at later time intervals (18 months and 2 year ...197897856
family life cycle resource center in the elementary school. 200198495
serologic typing of rickettsiae of the spotted fever group by microimmunofluorescence. 1978101593
clinical studies on pipemidic acid. 1978101691
health expenditure 1978/79. 1998104698
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
[unusual fetomaternal blood group antagonism]. 1998105299
[evolution and transformation in animal organism of some polychlorobiphenyls (author's transl)].intoxication by pure polychlorobiphenyls and a commercial product is studied, in rats, after intraperitoneal and oral administration. their fixation and excretion of their metabolites is greater by intraperitoneal than by oral administration. the only hydroxy metabolites, identified by means of synthesis, gas chromatography and mass spectrometry are, all, derivatives from trichlorobiphenyl. it appears that administration of commercial product induces a more rapid degradation of these compounds i ...1978105430
[x-ray study of the stomach. what is it used for and what is it worth?]. 1998105438
[isolation of treponemas from the colon of pigs with clinical dysentery].optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ...1979105448
[kinetics of the serological response in the experimental primary hydatid disease of mice infected with echinococcus granulosus embryophores (author's transl)].a serological response kinetics study was carried out in cf-1 mice orally infected with echinococcus granulosus embryophores using immuno-electrophoresis, double diffusion, latex agglutination and passive hemagglutination. starting from the 76th day after the infection, serum antibodies reach the minimum detectable level by immunoelectrophoresis test. band number 5, now considered as specific for e. granulosus, was proved to be the first precipitating system exhibited in this test. double diffus ...1978105664
cryopreservation of antibody-coated human erythrocytes. 1978105832
[general anesthesia and long term psychotropic treatments].anesthesists often ask for withdrawal of psychotropic treatment a few days before surgery. usually they do not differentiate among the various classes of psychotropic drugs. some drugs, including mao inhibitors and reserpine-like agents, can induce or precipitate accidents; however, this does not occur with other types of antidepressants, neuroleptics and minor tranquilizers. the authors review the psychiatric risks related to such withdrawals; these risks are to be considered. they report that ...1998105865
cerebrospinal fluid-, blood-, and ascitis-catecholamines in hepatic cirrhosis.the consideration of the extensive innervation of liver parenchyma, together with data relating to the degradation of catecholamines in the liver and the importance of hepatic vascular process in hepatic cirrhosis, prompted us to investigate the behaviour of catecholamines in this condition. the following parameters were measured in cerebrospinal fluid (csf), blood and ascitis fluid: total catecholamines, adrenaline and noradrenaline. during hepatic cirrhosis, total catecholamines level in blood ...1998106413
microscopic changes in rat tongue following experimental cryosurgery.histological changes were studied in the rat tongue for varying periods of time up to 14 days following the application of a cryoprobe to the dorsal surface of rat tongue. within minutes of the application, focal subepithelial vesiculation and hyperemia occurred. at 3 h there was evidence of damage to capillary walls, resulting in hemorrhage. arterioles and venules were thrombosed and there were degenerative changes in the muscle layer of their walls. at 6 h ventral epithelium showed evidence of ...1979108379
[fundamental studies and clinical evaluation of pc-904 in urinary tract infection (author's transl)].measurement of mics of pc-904, cbpc and gm against 135 strains of pseudomonas aeruginosa isolated from patients with urinary tract infections was performed. susceptibility to pc-904 of pseudomonas aeruginosa was found to be 3 approximately 7 times more sensitive than that to cbpc and as same as that to gm with inoculum size of 10(8)/ml and 10(6)/ml. serum level of pc-904 reached a peak of 38.5 microgram/ml 15 minutes after 0.5 g intravenous administration. urinary recovery rate unit 6 hours afte ...1979108428
quantitation of 3-ethyl-5-hydroxy-4,5-dimethyl-delta 3-pyrrolin-2-one in the urine of patients with acute intermittent porphyria. 2000110600
[pancreatic carcinoma on endoscopic retrograde pancreatico-cholangiogram (author's transl)].the pancreaticographic appearances of carcinoma of the pancreas have been divided into two types: 1. canalicular carcinoma arising from the duct system. if arising from the main pancreatic duct, it occludes this, or causes displacement or deformity of its branches in the immediate neighbourhood. if arising from branches, it causes cystic ectasia of the minor ducts; these appear fragmented and deformed while there is stenosis and displacement of the main duct. simultaneous origin from the main an ...1998124684
["masked" supra-renal tumour (author's transl)].for the diagnosis of supra-renal tumours or tumours of supra-renal rests, it is necessary to employ all available radiological methods as well as the clinical data. two cases are described in which a supra-renal tumour had been "masked" and found at operation. in the first case a cyst in the tail of the pancreas had been considered, in the second, a tumour of the renal hilum or ureter had been diagnosed. it is concluded that in the differential diagnosis of a cyst of the tail of the pancreas, su ...1998124685
k+, mg2+-aspartate (kmg-asp)--mediated prevention of isoproterenol(iso)-induced metabolic changes in the myocardium.isoproterenol (iso) in a single dose of 7.5 mg/kg b.w. i.v was found to produce alterations of cardiac metabolism in dogs. after 2 h, high energy phosphate stores and glycogen were reduced, whereas the levels of lactate, pyruvate, the lactate/pyruvate ratio and myofibrillar atpase activity were elevated. ca2+ -accumulation by sarcoplasmic reticulum (sr) and mitochondria were increased (p less than 0.01). after 24 h, a partial recovery in the parameters followed could be observed. only myofibrill ...1975126687
characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa.neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ...1975126993
[experience in combination of the educational and scientific work of students]. 2002127158
[pneumatization of the paranasal sinuses. causes and symptoms of hypopneumatization]. 2002127302
induced transfer of higher plant chloroplasts into fungal protoplasts.chloroplasts isolated from spinach leaves have been transferred in large numbers into protoplasts of neurospora crassa with the help of polyethylene glycol. the chloroplasts show high photosynthetic activity--at least until the time of uptake--and the protoplasts continue to show active cytoplasmic streaming after chloroplast uptake.2002127380
[clinical and biological aspects of chronic brucellosis].the present study refers to 53 cases of chronic brucellosis admitted to the clinic or followed up in an out-patient unit for ten years (1962-1971). there was a total of 946 days in hospital and 1688 days of occupational disability, about 40% of the patients necessitating repeated hospitalization because of the relapsing course of the disease. this group of cases presented the classical dominant features of an occupational anthropozoonoses (90.5%). elements of visceral and tissular pathology, obv ...2002128800
[50 years of the institute for experimental pathology and therapy in sukhumi]. 2003148825
[ivan andreevich spiriukhov (on his 70th birthday)]. 2003150266
purification of cytochrome b from neurospora crassa and other sources. 2003152379
[separation and characterization of subcellular organoids by tissue fractionation]. 1975152443
[postoperative esophageal stenosis (role of the laryngologist)]. 2002153373
resection of a massive arteriovenous malformation of the head and neck.we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case.2003154679
dl-2-keto-4-hydroxyglutarate-1. 2003165381
[pathological studies on corynebacterial ulcerative entero-colitis in rats treated with acth (author's transl)].ulcerative entero-colitis was developed in 6-week-old male sprague-dawley rats treated daily with acth (4 mg/kg. s. c.) as well as necrotic purulent lesions in liver, kidney, lung or heart. incidence of ulcerative lesions was 6.3% in farm-a rats and 56% in farm-b rats. although ulcerative lesions were mostly observed in cecum, the similar lesions were also detected in distal ileum or proximal colon in some cases. histologically, the lesions were characterized by focal necrosis demarcated from su ...1975174920
morphological correlates of synaptic transmission in lamprey spinal cord.the dye procion brown was used to identify in the light and electron microscope, synaptic contacts made between monosynaptically coupled neurons in the lamprey spinal cord whose synaptic interaction had been recorded. synaptic contacts were made on different dendrites of the postsynaptic cell at different distances from the soma. some of the contacts were made on dentritic spines and some on the smooth shaft of the dentrites. serial sections through synaptic contacts made on dendritic processess ...1985176330
adaptation of cells derived from human malignant tumours to growth in vitro.forty-five human malignant tumour specimens were cultivated in vitro in an attempt to determine the necessary conditions for tumour cell maintenance and to establish permanently-growing cell lines. continuously-growing cultures were derived from five tumours, including carcinomas of the oesophagus and colon, a hepatoma, a mesothelioma and a retroperitoneal sarcoma. the carcinoma of the oesophagus and the hepatoma, which have adapted fully to in vitro conditions, can be regarded as established ce ...1976184551
the accumulation of cyclic amp and cyclic gmp in guinea pig brain slices. effect of calcium ions, norepinephrine and adenosine. 1977195635
constant and variable regions in glycoprotein hormone beta subunit sequences: implications for receptor binding specificity. 1994201763
financial management of department of nursing services. 1990258786
secondary hypertriglyceridemia and hyperlipoproteinemia in patients with primary asymptomatic gout.we carefully selected 30 men with primary gout, rendered asymptomatic by therapy, to examine the frequency and type of hyperlipidemia and hyperlipoproteinemia, with the objective of determining whether serum uric acid, alcohol intake, liver function, kidney function, and (or) drugs were participating in the secondary lipid disorder. sixty-one age- and sex-matched men were used as controls. about 73% of the gout patients had hypertriglyceridemia, 1.6-fold the frequency found in the control group. ...1985262176
thyroid - hormone effects on steroid - hormone metabolism. 1977266410
the laboratory surveillance of influenza epidemics in the united kingdom 1968-1976.the extensive laboratory investigations of respiratory disease in the u.k. over many years have demonstrated the frequency with which influenza viruses, both a and b, are found each winter. only rarely are none isolated. these findings correlate well with other indicators of influenza such as increases in sickness benefit claims and in deaths attributed to influenza and pneumonia. however, outside these demonstrable peaks of incidence influenza viruses have been found to circulate over considera ...1977267671
reflection on geriatrics. 1990267819
hemoglobin e and glucose-6-phosphate deficiency in the khmer air force (cambodia).this study was designed to investigate the use of primaquine in malaria control in the khmer republic (cambodia). blood was drawn from 106 male khmer air force troops for analysis of hemoglobin e and glucose-6-phosphate (g-6-pd) deficiency. a test group of 15 men with g-6-pd deficiency and a group of 31 normal controls were given 15 mg of primaquine each morning for 14 days. the patients were followed for hemoglobinuria and changes in hematocrit. primaquine induced a significant, but not a dange ...1990268155
the ada conference on increasing access to dental care. a report. 1985268525
[fine structure of oral spirochetes, especially axial fibrils and cyst like structure (author's transl)]. 1977268542
[specialization in periodontology].in addition to the demand that periodontal therapy should be the task of any stomatologist, the considerable morbidity and the aetiopathological special features of periodontal diseases call for specialists. periodontal basic care and specialized therapy easy to delimitate. the specified stomatological curriculum allows for the demands on periodontology; it is imperative to enforce them in education and continuing education.1986268701
the immediate overlay denture. 1987269213
primary papillary carcinoma of the prostate: report of a histopathologic, cytologic and electron microscopic study on one case.an unusual type of prostatic carcinoma is described. histopathologic examination showed a papillary and acinar appearance. aspiration cytology showed papillary fragments and sheets of malignant cells. the ultrastructural features of this tumor were similar to those reported for prostatic carcinoma rather than endometrial carcinoma supporting a prostatic orgin for this tumor.1990269605
[mycoplasma infections]. 1977269619
dermoid cyst of the floor of the mouth in an infant. (case report).dermoid cysts of the floor of the mouth in the infant are rarely encountered although the aetiology is mainly embryological. meyer proposes the classification of the cysts based on the histological differentiation of the elements of the cyst wall. a case is reported which shows differences in the epithelial lining of the cysts found followed by the differential diagnosis and treatment.1991269895
the value of a simple medical questionnaire in dentistry.a short specific medical questionnaire was given to 1125 new patients attending for their initial examination at the perth dental hospital. a positive history was given by 398 patients. a wide variety of conditions was revealed and the dental significance is discussed. the extent of the information provided illustrates that the simple questionnaire is valuable in exposing conditions which may affect dental treatment.1984270975
diabetes mellitus and periodontal disease. 1981273875
different effects of the ionophore a-23187 and d-glucose on 45ca2+ fluxes in isolated islets of ob/ob-mice.fluxes of 45ca2+ were studied in beta-cell rich islets of non-inbred ob/ob-mice, using lacl3 to wash out extra-cellular and superficially bound 45ca2+. the ionophore a-23187 (10 microm) increased the 45ca2+ uptake in islets both at 3 and 20 mm d-glucose, the effect being more pronounced after 10 min than after 120 min of incubation. in incubations for 120 min, 20 mm d-glucose induced a higher uptake of 45ca2+ than did a-23187. the ionophore enhanced the unidirectional efflux of 45ca2+ from prelo ...1986362823
fred jenner hodges, ii, m.d. 1895--1977. 2004375293
homogeneous immunoglobulins in the serum of irradiated and bone marrow reconstituted mice: the role of thymus and spleen.the influence of thymectomy and splenectomy on the frequency and class distribution of homogeneous immunoglobulins (h-ig) in serum was studied in lethally irradiated (dba/2 x c57bl/rij)f1 mice reconstituted with syngeneic bone marrow. during four follow-up periods in the first 9 months after transplantation, the sham-operated controls and splenectomized animals developed transient h-ig in an average frequency of 14.2 and 15.7% respectively. there were no marked differences in the incidence of h- ...2004387578
effect of high-energy feedings on low birthweight infants.a comparison of the effect of 100.5 kj/30 ml (24 kcal/30 ml) feedings with that of 84 kj/30 ml (20 kcal/30 ml) feedings on infants with a birthweight of 1500 g or less was made. no consistent benefits in growth occurred and there was a tendency for an increase in complications in infants fed the 100.5 kj/30 ml milk. hence the higher-energy feeding cannot be recommended as the routine feeding for low birthweight infants.2004470692
[bilirubin in the blood serum of clinically healthy cows and those ill with ketosis and hepatopathies].studied was the bilirubin equilibrium in 62 normal cows, 52 cows with clinical ketosis, 31 cows with diffuse liver injury, and 14 cows that went off food for five days. a moderate rise of the total bilirubin of mixed type was found in ketosis-affected cows. statistically significant proved the changes in the free fraction, however, there were no persuasive data that they were due to superproduction of bilirubin. changes in the total bilirubin similar to those in cows with ketosis were establishe ...1979516442
[ultrasonic localization for closed renal biopsy]. 2004520913
a simple centrifuge column for desalting protein solutions. 2005543535
geriatric patient care. opinions of a sample of nurses. 20051134987
[extracellular nucleases of phytopathogenic bacteria]. 20051214627
[salmonellosis carrier state]. 19751214656
[personality of patients with myocardial infarct]. 20051236645
nucleotide sequences and characterization of haemolysin genes from aeromonas hydrophila and aeromonas sobria.extracellular haemolysin is thought to be one of the important virulence factors in aeromonas infection. two extracellular haemolysin genes (ahh3 and ahh4) from aeromonas hydrophila strain 28sa, one (ahh5) from a. hydrophila strain ah-1 and one (asa1) from aeromonas sobria strain 33 were cloned into cosmid and plasmid vector dna in escherichia coli. the nucleotide sequences of the open reading frames of ahh3 and ahh4 are both 1476 basepairs (bp), whereas ahh5 and asa1 are 1455 and 1467 bp in len ...19921302284
scintigraphic study of the gastrointestinal transit of a liquid meal in patients with chronic chagas' disease.gastric emptying and small bowel transit of a liquid meal (isotonic dextrose) were assessed by a scintigraphic technique in 16 patients with chagas' disease involving the esophagus and/or the colon, including one case with mega-jejunum, and in 10 control subjects. initial gastric emptying was faster in the chagas' disease group than in controls (gastric retention at 15 min, median and (range): 52% (15-86) vs 71% (43-97), p < 0.01) although there was no significant difference in total gastric emp ...19861339510
characterization of aeromonas sobria tap13 pili: a possible new colonization factor.pili of aeromonas sobria tap13 were purified and characterized. the molecular mass of the pilin was estimated to be about 23 kda by sds-page. the tap13 pili were immunologically different from a. sobria ae1 pili and a. hydrophila ae6 w pili as previously reported, nevertheless all three had indistinguishable morphology and shared a high degree of homology in their n-terminal amino acid sequences. strain tap13 and its purified pili did not agglutinate human, rabbit or sheep erythrocytes. however, ...19921357078
purification and characterization of aeromonas sobria ae24 pili: a possible new colonization factor.pili of aeromonas sobria ae24 were purified and characterized. the molecular mass of the pilin was estimated to be about 19 kda by sds-page. the ae24 pili were electrophoretically distinguishable from previously reported aeromonas hydrophila ae6 w pili and a. sobria ae1 pili, although all three had indistinguishable morphology and shared a high degree of homology in the n-terminal amino acid sequences. strain ae24 and its purified pili adhered to rabbit intestine and agglutinated human and rabbi ...19921363704
isolation and characterization of a cryptic plasmid from mesophilic aeromonads: potential as a cloning vector.a 2.6 kb covalently closed circular plasmid has been isolated from clinical and environmental isolates of aeromonas sobria and a. hydrophila. the possibility that the plasmid carries genetic determinants that mediate resistance to a variety of anti-microbial agents has been eliminated. the plasmid is stable at approximately 20-25 copies per chromosome equivalent which, together with its relatively small size and the presence of unique restriction sites, makes it a good candidate for development ...19911366999
a high containment polymodal pilot-plant fermenter--design concepts.a 225 dm3 pilot-plant bioreactor system has been designed and constructed that is suitable for biohazardous fermentations. the design enables operation at containment levels above the requirements of good industrial large-scale practice (gilsp) without secondary containment of the whole plant. the main biosafety features of the systems include the use of steam barriers on o-ring seals, supply lines and stirrer seals, multiple o-ring seals, piping of condensate lines and pressure relief systems t ...20061367227
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
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