Publications

demonstration of an alpha2-macroglobulin receptor in human fibroblasts, absent in tumor-derived cell lines.		1979	87392
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.	the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...	1979	89900
projections of auditory nerve in the cat as seen by anterograde transport methods.		1979	91142
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.	the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...	1979	104958
a new principle of injectable depot contraceptives. i drug selection and studies in monkeys.	the aim of the investigations was to develop a long-acting depot contraceptive on the basis of norethisterone or levonorgestrel. disappointing results with levonorgestrel nonanoate and levonorgestrel undecylate showed that elongation of the fatty acid, esterified with the steroid, decreased the bioavailability of the latter due to incomplete hydrolysis of the ester. therefore, several new compounds were synthesized which contained a bifunctional molecule between the steroid and the fatty acid. i ...	1979	110513
crosslinking studies on the ca2+, mg2+-activated atpase of escherichia coli.	crosslinking of membrane proteins of escherichia coli with dithiobis (succinimidyl propionate) (dsp) resulted in loss of several enzyme activities including the ca2+, mg2+-activated atpase. this enzyme was crosslinked by dsp to the membrane and was not released by dialysis at low ionic strength in the absence of dithiothreitol which could cleave the crosslinking group. dsp inactivated both phosphohydrolase and coupling activities of the solubilized atpase. loss of hydrolytic activity could be co ...	1975	127090
v. brown university alcoholism conference: a summary of the discussions.		2002	127377
[some epidemiological factors in the incidence of female genital cancer in the azerbaijan ssr].	in the structure of cancer morbidity of the female genitalia in azerbaijan ssr within a ten-year period (1957-1966) cervical cancer occupies the first place--86.6%, it is observed more frequently among russian female population (46.4 per 100 000 wives) and more rarely among aboriginal female population (7.1 per 100 000 wives). of 2488 patients with cervical cancer 24.5% showed previous lesions against which background cancer would arise. patients with cervical ruptures, advanced erosions, polyps ...	2002	127423
dissociated cultures of newborn mouse brain. i. metabolism of sulfated lipids and mucopolysaccharides.	the metabolism of 35so4-sulfated lipids and mucopolysaccharides was studied in dissociated brain cell cultures from newborn albino mouse brains. the cultures were maintained under an atmosphere of 40% o2 and 5% co2 in apparent good health up to 30 days. early morphological examination of the dissociated cells demonstrated an initial partial reaggregation of the cells, which later settled and became confluent bilayered cultures. cell proliferation measured by dna and protein determination, morpho ...	1975	128706
resection of a massive arteriovenous malformation of the head and neck.	we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case.	2003	154679
the crystal structure of bonito (katsuo) ferrocytochrome c at 2.3 a resolution. ii. structure and function.	the structure analysis of bonito heart ferrocytochrome c was carried out at 2.3 a resolution by x-ray diffraction, and a kendrew-type skeletal model was built up. this molecule has an overall egg shape, 35 a in height, 30 a in width and 23 a in thickness; the 5th ligand of the heme iron atom is the n-epsilon atom of the his-18 imidazole ring and the 6th is the met-80 sulfur atom. distinct alpha-helix regions are found between the n-terminus and reside 11, between 60 and 69, and between 90 and th ...	1975	166072
[melanotic progonoma. report of two cases (author's transl)].		2003	182628
polymyxins as inhibitors of polyclonal b-cell activators in murine lymphocyte cultures.	the polymyxin antibiotics polymyxin b sulfate and colistin methane sulfonate were examined for their ability to inhibit responses to the polyclonal b-cell activators (pba) bacterial lipopolysaccharide (lps), dextran sulfate (ds), pneumococcal polysaccharide (siii), and purified protein derivative of tuberculin (ppd) in spleen cell cultures. polymyxin concentrations of 1 and 10 microng/ml significantly inhibited both the deoxyribonucleic acid synthetic and polyclonal antibody responses stimulated ...	2003	193787
isoprenaline-induced release of cyclic adenosine 3',5'-monophosphate by the left ventricle in the anaesthetized intact dog.	the ability of the heart to release cyclic adenosine 3',5'-monophosphate (cyclic amp) in the coronary venous blood was examined in closed-chest anaesthetized dogs before and during an infusion of isoprenaline. before the infusion, no difference was observed between the cyclic amp plasma levels in the aortic and coronary sinus blood. during the infusion, significant coronary veno-arterial differences in nucleotide concentration were observed despite a considerable elevation of the cyclic amp plas ...	1977	201223
oxidation of protein sulfhydryls by products of peroxidase-catalyzed oxidation of thiocyanate ion.		1978	204336
health care in china. 2.		2004	310187
health care in china. 1.		2004	310189
intra-osseous lipoma of the proximal part of the femur. case report.		2004	374417
stereo-specific synthesis of 3-trifluoromethylcephalosporin derivative by microbial acylase.	cephalosporin acylase (ec 3.5.1.11) obtained from kluyvera citrophila atcc 21285 was found to catalyze synthesis of 7-[2-(2-thienyl)acetamido]-3-trifluoromethyl-3-cephem-4-carboxylic acid from methyl thienylacetate and dl-7-amino-3-trifluoromethyl-3-cephem-4-carboxylic acid. the enzymatically-synthesized compound showed [alpha]25 d + 42.7 degrees (c 0.058, meoh) and its biological activity was about twice as much as that of racemic 7-[2-(2-thienyl)acetamidol]-3-trifluoromethyl-3-cephem-4-carboxy ...	1979	393680
[a simple method for the determination of pcb in human milk and blood using an improved essential oil distillator (author's transl)].		1977	404452
[ultrasonic localization for closed renal biopsy].		2004	520913
echocardiographic features of right ventricular volume overload.		2004	529179
a controlled study of the perception of body width in anorexia nervosa.		2004	529199
[fractures and luxations in the pelvic region. urogenital injuries (author's transl)].		2005	538870
[association of monoclonal gammopathy with chronic glomerulonephritis and autoimmune hyperlipemia. course of disease during therapy].	a case of benign monoclonal gammapathy associated with an auto-immune hyperlipidaemia and a chronic glomerulopathy is reported. extraction of the monoclonal immunoglobulin made it possible to demonstrate its anti-lipoprotein antibody activity. parallel changes in serum lipid levels and monoclonal immunoglobulin levels under the influence of melphalan confirmed the direction relationship between the lipid and protein disorders. the pathogenesis of the glomerulopathy and of the massive and prolong ...	2005	1068625
[kartagener's syndrome in two siblings].		2005	1087624
penicillin acylase mutants with altered site-directed activity from kluyvera citrophila.	oligonucleotide-directed mutagenesis has been used to obtain specific changes in the penicillin acylase gene from kluyvera citrophila. wild-type and mutant proteins were purified and the kinetic constants for different substrates were determined. mutations in met168 highly decreased the specificity constant of the enzyme for penicillin g, penicillin v and phenylacetyl-4-aminobenzoic acid and the catalytic constant kcat for phenylacetyl-4-aminobenzoic acid. likewise, the phenylmethylsulphonyl-flu ...	1990	1366784
changing glycine 21 for glutamic acid in the beta-subunit of penicillin g acylase from kluyvera citrophila prevents protein maturation.	active penicillin acylase from kluyvera citrophila strain atcc 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. using the chemical mutagen hydroxylamine we have treated plasmid pykd59 containing the active penicillin acylase gene (pga) from k. citrophila and have generated different point mutant penicillin acylase genes, one producing a maturation deficient precursor. this point mutation has changed the glycine 310 residu ...	1992	1368070
health checks on patients 75 years and over in nottinghamshire after the new gp contract.	to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.	2006	1393076
sleep organization and energy expenditure of breast-fed and formula-fed infants.	sleep organization of infants may be influenced by differences in nutrient intakes from human milk and formula. because sleep/awake and sleep stage patterns affect energy expenditure, we hypothesized that differences in sleep organization between breast-fed and formula-fed infants might account in part for differences in energy expenditure between feeding groups. sleep stages and cycling of 4-mo-old breast-fed (n = 10) formula-fed (n = 10) infants were studied with simultaneous measurements of e ...	1992	1480450
a framework for the knowledge-based interpretation of laboratory data in intensive care units using deductive database technology.	in co-operation with the institute of anaesthesiology of the ludwig-maximilians-university in munich a computer-based system for the analysis and interpretation of renal function, fluid and electrolyte metabolism of critical care patients has been developed. this paper focuses on the requirements and implementation aspects of the knowledge-based interpretation for this particular system. objective of the proposed approach is, to transform an enormous--and constantly increasing--amount of raw dat ...	2006	1482854
hyperoncology: demonstration of an evolving comprehensive hypermedia-based workstation for the radiation oncologist.	hyperoncology is a prototype hypermedia-based workstation designed for the practicing radiation oncologist. individual patient records consist of linked demographic, clinical history, presentation, staging, course, laboratory and treatment data. searching and sorting capabilities, navigational aids, information printing and archiving, plotting capability, and graphics and updating tools are available. upon integration with an evolving knowledge base, hyperoncology will eventually become a compre ...	2006	1483010
chemical modification of serine at the active site of penicillin acylase from kluyvera citrophila.	the site of reaction of penicillin acylase from kluyvera citrophila with the potent inhibitor phenylmethanesulphonyl fluoride was investigated by incubating the inactivated enzyme with thioacetic acid to convert the side chain of the putative active-site serine residue to that of cysteine. the protein product contained one thiol group, which was reactive towards 2,2'-dipyridyl disulphide and iodoacetic acid. carboxymethylcysteine was identified as the n-terminal residue of the beta-subunit of th ...	1991	1764029
a review of the safety profile of teicoplanin.	safety of teicoplanin has been assessed in 3377 patients treated in europe up to the end of june 1990. one or more adverse events were experienced by 10% of patients. age and teicoplanin dose had no significant effect on the incidence or type of adverse event. in comparative trials the incidence and profile of adverse events to teicoplanin have been similar to those seen with beta-lactam therapy. impaired renal function occurred consistently more frequently with vancomycin therapy than with teic ...	2006	1829079
thermodynamic profiles of penicillin g hydrolysis catalyzed by wild-type and met----ala168 mutant penicillin acylases from kluyvera citrophila.	the met-168 residue in penicillin acylase from kluyvera citrophila was changed to ala by oligonucleotide site-directed mutagenesis. the ala-168 mutant exhibited different substrate specificity than wild-type and enhanced thermal stability. the thermodynamic profiles for penicillin g hydrolysis catalyzed by both enzymes were obtained from the temperature dependence of the steady-state kinetic parameters km and kcat. the high values of enthalpy and entropy of activation determined for the binding ...	1990	2306469
'the cost benefit of routine pre-operative hb investigations for oral surgery'.		2008	2493261
the human calbindin 27-kda gene: structural organization of the 5' and 3' regions, chromosomal assignment, and restriction fragment length polymorphism.	the 5' and 3' regions of the human gene coding for calbindin 27 kda were cloned and sequenced. structural features of the 5' region included the presence of an alu repeat and two elements regularly associated with eukaryotic promoters: an alternating purine-pyrimidine element and a homopurine-homopyrimidine box. the 3' region contained a second alu family member and a degenerate 1.4-kb l1 repeat. a comparison with the chicken promoter was made in order to define regions conserved in evolution an ...	1989	2565876
ultrathin (1 nm) vertically shadowed platinum-carbon replicas for imaging individual molecules in freeze-etched biological dna and material science metal and plastic specimens.	single molecule resolution in beam-sensitive, uncoated, noncrystalline materials has heretofore not been possible except in thin (less than or equal to 150 a) platinum-carbon (pt-c) replicas, which are resistant to electron beam destruction. previously, the granularity of metal film replicas limited their resolution to greater than or equal to 20 a. this paper demonstrates that pt-c film granularity and resolution are a function of the method of replication and other controllable factors. low-an ...	2010	2809773
neurothermography.	neurothermography is a new, clinically useful method of detecting autonomic sympathetic peripheral nerve dysfunction. body symmetry of the skin temperature as maintained by symmetrically functional peripheral nerves is the key to detecting a normal state. persistent 1 degree c temperature asymmetry in the pattern of a peripheral nerve (thermatome) is a highly reliable method of detecting autonomic peripheral nerve disorder.	2010	2845550
complete nucleotide sequence of the penicillin acylase gene from kluyvera citrophila.	the penicillin acylase (pac) from kluyvera citrophila atcc21285 has been purified to homogeneity and found to be composed of two non-identical subunits of 23 and 62 kda, in contrast with the previous findings [shimizu et al., agr. biol. chem. 39 (1975) 1655-1661]. the nucleotide (nt) sequence of the k. citrophila pac gene contained in the 3-kb pvui-hindiii fragment of pkap1 [garcía and buesa, j. biotechnol. 3 (1986) 187-195] has been determined, showing that it encodes a protein of 844 amino aci ...	1986	3032748
indirect immunofluorescence of oral lichen planus.	sections of lesional tissue from 6 patients who exhibited only oral lesions of lichen planus (lp) were incubated with autologous serum and studied immunopathologically for the presence of lichen planus specific antigen (lpsa). none of the specimens was positive for lpsa. serum from the oral lp patients was subsequently incubated with allogeneic cutaneous lp lesional tissue sections, which resulted in the demonstration of lpsa in 2 of the 4 sera examined. these results suggest an immunologic rela ...	2011	3088237
called to account.		2010	3105676
[primary leiomyosarcoma of the pulmonary artery. apropos of a case].	a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ...	1987	3122693
phospholipase c activates protein kinase c and induces monocytic differentiation of hl-60 cells.	phospholipase c (plc)-mediated hydrolysis of membrane phospholipids results in the production of diacylglycerol, inositol phosphates, and choline metabolites. inositol triphosphate increases calcium levels, while diacylglycerol activates protein kinase c. the present studies demonstrate that exogenous plc generates inositol phosphates, releases choline metabolites, and activates protein kinase c in human hl-60 promyelocytic leukemia cells. plc also induced monocytic differentiation of hl-60 cell ...	1988	3165300
experimental infection of sheep and goats with muellerius capillaris (protostrongylidae, nematoda).		1988	3188728
[properties of lak cell activity and lak-generation in pbl isolated from patients with renal cell carcinoma].		2012	3266646
computers and dental health education--a trial project at the liverpool garden festival.		2012	3297819
comparison of segmental pancreatic transplantation with duct obstruction and pancreaticoduodenal transplantation with enteric diversion.		2012	3313861
epidemiology of allergic contact sensitization.		2012	3316993
the unborn patient.		1988	3347563
a kluyvera cryocrescens strain from a gall-bladder infection.	the isolation and the identification of a pure-culture kluyvera cryocrescens strain in a gall-bladder pus specimen from a 76-year-old woman with acute cholecystitis is described. this is the first reported recovery of a k. cryocrescens strain from such a sample.	1988	3356228
biometeorological triggers in childhood asthma.	the relationship between child asthma admissions and biometeorological factors was investigated over a 16-month period. no relationship was found with many commonly suspected precipitants such as temperature, humidity and wind. there was however a strong association with rainfall (p much less than 0.001). associations occurred with low barometric pressure and counts of coloured basidiospores and green algae, but no significant relationship was found with grass pollen.	2012	3416418
differentiation of kluyvera cryocrescens from kluyvera ascorbata by irgasan susceptibility testing.	from the faeces of a diarrhoeic patient, we isolated a strain of kluyvera ascorbata on cefsulodin-irgasan-novobiocin (cin) agar, a highly selective medium used for yersinia enterocolitica. since no selective media have been described for kluyvera spp., we tested 5 strains each of k. ascorbata and k. cryocrescens for their ability to grow on cin agar. k. ascorbata exhibited an inhibition on cin of less than 10% cfu compared to blood agar, whereas k. cryocrescens did not grow at all even with inoc ...	1986	3426156
alternative protein products with different carboxyl termini from a single class i gene, h-2kb.	in addition to mrna encoding the canonical form of the murine class i antigen h-2kb (348 amino acid residues), mrna that would encode a shortened form of h-2kb (missing 9 amino acids from the c-terminus) has been identified in c57bl/6 spleen cells by rnase-protection studies. the alternative transcripts of h-2kb arise through the use of different ag acceptor splice sites for exon viii. the existence of a shortened h-2kb protein was demonstrated by sequential immunoprecipitation. lysates of splee ...	1986	3461476
protostrongylus rufescens: a cytogenetic study.	the diploid chromosome number of protostrongylus rufescens is 2n = 11 for males and 2n = 12 for females. so, the sex determinism mechanism is xo/xx. the study of the genetic behaviour of this species has been made. in diakinesis stage the bivalents show typical tetrads with cross, phi, and lineal configurations. the division of the sexual chromosome is prereductional for the first meiotic division.	1987	3571924
parthenium dermatitis sparing vitiliginous skin.		2013	3581827
effects of peptide yy on gallbladder motility.	the effects of peptide yy (pyy) on cholecystokinin-stimulated gallbladder contraction were investigated in the prairie dog model. twelve animals underwent laparotomy with catheter placement into the gallbladder and common bile duct (vent). the gallbladder was continuously perfused with [14c]polyethylene glycol-labeled lactated ringer at 0.03 ml/min, and vent effluent was collected at 2.5-min intervals. all animals received 20 min of intravenous infusion of cholecystokinin octapeptide (cck-op), 2 ...	1987	3591942
broviac catheter infection with kluyvera cryocrescens: a case report.	kluyvera cryocrescens was isolated from a blood culture drawn from a patient with a broviac catheter-related infection. k. cryocrescens has been considered an opportunistic pathogen but has not previously been associated with central venous catheter infections.	1987	3597755
psychophysical maps for subadditive dissimilarity ratings.		2013	3684491
social, economic, and surgical anatomy of a drug-related abscess.	the "letting of pus" has been a surgical triumph throughout medical history. this study was initiated to expand our knowledge of the etiologic, economic, and surgical aspects of an abscess. the records of 651 patients undergoing intraoperative incision and drainage of an abscess over a 12-month interval were analyzed. the abscesses were due to injection of illicit street drugs in 421 patients (64.7%), perirectal, pilonidal or sweat gland inflammation in 118 patients (18%), complications of diabe ...	1986	3729176
[renal osteodystrophy in children with chronic renal insufficiency].		2012	3769023
correlation between auditory reaction time and intelligence.	discriminative reaction times to auditory stimuli were measured for 150 students of the local chinese population. pearson's correlation with scores on the standard progressive matrices and rts was negative, becoming -0.30 when slower trials were eliminated. the possible basis for improvement was discussed in relation to cognitive processes and the general theory of assessing intelligence.	2013	3774443
sweat gland response to local heating during sleep in man.	in order to assess whether the fluctuations in the sweating response occurring during sleep are related to changes in central drive or in peripheral sweat gland reactivity, 4 healthy male subjects spent 6 non-consecutive nights in a climatic chamber. air temperature was 25 degrees c, dew-point temperature was 10 degrees c and air velocity was 0.3 m x s-1, while wall temperature was either 38 degrees c, 46 degrees c or 48.7 degrees c giving 3 levels of operative temperature (to = 30, 33 or 34 deg ...	1986	3795118
determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection.		1985	3907412
splenic syndrome at mountain altitudes in sickle cell trait. its occurrence in nonblack persons.	six consecutive cases of splenic syndrome at mountain altitudes in persons with sickle cell trait are reported and the literature is reviewed. all six cases occurred in men who experienced the acute onset of severe left-upper-quadrant abdominal pain within 48 hours of arrival in colorado from lower altitudes. all six patients were phenotypically nonblack. three patients experienced their symptoms at moderate altitudes of 1,609 to 2,134 m (5,280 to 7,000 ft) above sea level. all recovered with me ...	2014	3974118
day to day variations in morphology and duration of fragmented ventricular potentials during the late post-myocardial infarction phase in conscious dogs.	the relationship between the inducibility of ventricular tachyarrhythmias and the presence of fractionated epicardial ventricular electrograms ('late potentials') was studied daily between days 3 and 8 after experimental myocardial infarction in 15 conscious dogs. before each programmed stimulation, epicardial infarct zone electrograms were recorded from implanted 'composite' electrodes during sinus rhythm. there was considerable daily variation in the morphology and duration of delayed ventricu ...	1985	4054137
physiological stress and performance changes in response to beginning level orienteering.		2014	4068683
some findings on the pathology of the motor nerves of the limbs during medical diseases: the possibility of proximal involvement with conservation of distal excitability.		2012	4103553
education and the hospital: hospital office promotes health education.		2014	4119583
electron microscopic structure of the "inclusion bodies" in pick's disease.		2014	4178657
continuous splitting off of phosphate from phosvitins by phosphoprotein phosphatase.		1969	4309716
test method for determining the viricidal activity of disinfectants against vesicular stomatitis virus.	a test method using embryonating chicken eggs for the evaluation of several types of disinfectants as viricides against vesicular stomatitis virus was developed which gave specific, reproducible results.	1970	4313316
[functioning and non-functioning tumors of the insular pancreas. pathology, clinical aspects and therapy].		2013	4320215
[musculus rectus labii and its significance for the lip mobility of chimpanzees (pan troglodytes)].		2015	4451282
catabolism of hamamelose. the anaerobic dissimilation of d-hamamelose by kluyvera citrophila 627.		1974	4452358
[achievements in urology in the ussr].		2015	4592854
[the synthesis of rat liver and kidney lysosomes (author's transl)].		1974	4594653
homologous scleral explant buckles in retinal detachment surgery.		2015	4594665
stimulation of cell-free polypeptide synthesis by a protein fraction extracted from chick oviduct polyribosomes.		1972	4622202
the histaminase activity of ceruloplasmin.		2015	4630290
tuberous sclerosis and schizophrenia.		2015	4648445
giardia intestinalis. a prospective investigation of the prevalence in denmark.		1973	4697076
role of mental imagery in free recall of deaf, blind, and normal sunjects.		2015	4705238
[alpha 1 - antitrypsin, a modern glycoprotein].		2016	4733188
[new research on the inhibitory action of methyl-3 hydroxy-6 quinazoline dione 2-4 on the cytodierese of plant cells. duration of the cell cycle and mitosis].		2016	4802674
[mirin dajo].		2016	4874675
[supplement to the study of the mechanism of electrocardiographic alterations in meningeal hemorrhage (experimental study)].		1969	4975492
enzymatic synthesis of d(-)-alpha-aminobenzylpenicillin by kluyvera citrophila.		1971	5581352
purification and properties of hamamelosekinase.	hamamelosekinase (atp:hamamelose 2(1)-phosphotransferase) was purified from a crude extract of kluyvera citrophila 627 (enterobacteriaeceae) which has been grown on d-hamamelose. ammonium-sulfate fractionation and twofold chromatography on deae-cellulose resulted in a 51-fold purification of the enzyme. neither glucosekinase nor significant atpase activity could be detected in the pure preparation. besides d-hamamelose only d-hamamelitol was utilized as a substrate; however, the latter was phosp ...	1980	6249593
kluyvera, a new (redefined) genus in the family enterobacteriaceae: identification of kluyvera ascorbata sp. nov. and kluyvera cryocrescens sp. nov. in clinical specimens.	kluyvera is proposed as a new genus for the group of organisms formerly known as enteric group 8 (synonym = api group 1). strains of kluyvera share the properties of most members of the family enterobacteriaceae: they are gram-negative rods, motile with peritrichous flagella, catalase positive, and oxidase negative; they grow on macconkey agar, ferment d-glucose with the production of acid and gas, and are susceptible to many antibiotics. strains are usually indole positive, methyl red positive, ...	1981	7240403
new insights on the specificity of penicillin acylase.	in contrast with the general thought that penicillin g acylases (pgas) were only able to hydrolyse amides or esters of higly hydrophobic acids, we have demonstrated that the pga from kluyvera citrophila catalysed the hydrolysis of 4-nitrophenyl esters of acetic, propionic, butyric and valeric acids. values of kcat. and kcat./km were greatest for the first compound and less than values for benzylpenicillin by factors of 30 and 7, respectively. 4-nitrophenyl acetate was hydrolysed faster than 2-ni ...	1995	7826381
changing the substrate specificity of penicillin g acylase from kluyvera citrophila through selective pressure.	escherichia coli (mut, mutd, leu-) cells transformed with plasmid pykd59 harbouring the pac gene encoding penicillin acylase (pa) from kluyvera citrophila atcc 21285 were exposed to environmental conditions that made expression of this enzyme essential for growth. under these conditions, spontaneous mutants were isolated that used adipyl-l-leucine as the sole source of l-leucine. dna sequencing of the mutant pac genes identified a transversion mutation of thymine to guanine at position 1163. thi ...	1994	7980457
ph studies to elucidate the chemical mechanism of penicillin acylase from kluyvera citrophila.	the variation with ph of the kinetic parameters of penicillin acylase from kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. the ph-dependence of log (v/km) for penicillin g showed that a group with a pk value over 4.7 must be deprotonated and that a group with a pk value over 9.7 must be protonated in the free enzyme for activity. the solvent perturbation and temperature studies indicated that these groups are respectivel ...	1993	8338640
characterization of an escherichia coli aromatic hydroxylase with a broad substrate range.	the hpab gene encoding an aromatic hydroxylase of escherichia coli atcc 11105, a penicillin g acylase-producing strain, has been cloned and expressed in e. coli k-12. this gene was located near the paca gene coding for penicillin g acylase. the hydroxylase has a molecular mass of 59,000 da, uses nadh as a cosubstrate, and was tentatively classified as a 4-hydroxyphenylacetic acid hydroxylase, albeit it exhibited a rather broad substrate specificity acting on different monohydric and dihydric phe ...	1993	8458860
inactivation of penicillin acylase from kluyvera citrophila by n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline: a case of time-dependent non-covalent enzyme inhibition.	penicillin acylase (pa) from kluyvera citrophila was inhibited by n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (eedq), a specific carboxy-group-reactive reagent. enzyme activity progressively decreased to a residual value depending on eedq concentration. neither enzymic nor non-enzymic decomposition of eedq is concomitant with pa inactivation. moreover, enzyme re-activation is achieved by chromatographic removal of eedq, ph increase or displacement of the reagent with penicillin g. it was then ...	1993	8489517
rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin g acylase from kluyvera citrophila. effects of mutation f360v on rate constants for acylation and de-acylation.	the kinetics of release of 4-nitrophenol were followed by stopped-flow spectrophotometry with two 4-nitrophenyl ester substrates of penicillin g acylase from kluyvera citrophila. with the ester of acetic acid, but not of propionic acid, there was a pre-steady-state exponential phase, the kinetics of which were inhibited by phenylacetic acid (a product of hydrolysis of specific substrates) to the extent predicted from ki values. this was interpreted as deriving from rapid formation (73 mm-1.s-1) ...	1996	8687381
a case of kluyvera cryocrescens peritonitis in a capd patient.		1996	8761550
[utility of pyrrolidonyl-arylamidase detection for typing enterobacteriaceae and non-fermenting gram-negative bacteria].	detection of pyrrolidonyl-aryl-amidase activity (pyr) is an important tool to identify gram-positive cocci, such as staphylococci, enterococci, streptococci, and other related genera. however, only few studies evaluating its usefulness with gram-negative rods have been published. thus, a prospective study including 542 and 215 unique clinical isolates of enterobacteriaceae and non-fermentative gram-negative rods, respectively, was undertaken. strains were identified by conventional methods. pyr ...	1995	8850133
identification of the pac promoter from kluyvera citrophila.	the nucleotide sequence of the 5'-terminal region of the pac gene encoding the penicillin g acylase from kluyvera citrophila atcc 21285 has been determined. the transcriptional start site has been identified by primer extension analysis in a different position to that previously found for the homologous pac gene of escherichia coli w atcc 11105. two nucleotide changes in the -35 box appear to be responsible of the promoter displacement in k. citrophila. a putative upstream promoter element (a+t- ...	1997	9198278
kluyvera bacteriophage kvp1: a new member of the podoviridae family phylogenetically related to the coliphage t7.	a dna containing bacteriophage, kvp1, was isolated from the water of a very polluted river, the matanza river, near the central district of buenos aires city. this bacteriophage infects bacteria belonging to the kluyvera cryocrescens species (strain 21 g) isolated from the same river. kvp1 is a lytic bacteriophage and its propagation characteristics are: burst size 30, latent period 13 min and rise period 10 min. morphologically, kvp1 is a small icosahedral bacteriophage, 59.1 nm in diameter, wh ...	1997	9381794