Publications

nucleotide sequences of membrane-bound hydrogenase gene in alcaligenes hydrogenophilus.	the nucleotide sequences of membrane-bound hydrogenase small (hups) and large (hupl) subunit genes of hydrogen bacterium alcaligenes hydrogenophilus were determined. the hups and hupl genes encoded polypeptides of 363 and 619 amino acids, respectively. the hups was located upstream of hupl with 35bp of intergenic region. the consensus ribosome-binding sequences were identified upstream of the start codons of hups and hupl. amino acid sequence of hups is very similar to that of rhodobacter capsul ...	1992	1294332
cloning of pmol28-encoded nickel resistance genes and expression of the genes in alcaligenes eutrophus and pseudomonas spp.	the 163-kilobase-pair (kb) plasmid pmol28, which determines inducible resistance to nickel, cobalt, chromate, and mercury salts in its native host alcaligenes eutrophus ch34, was transferred to a derivative of a. eutrophus h16 and subjected to cloning procedures. after tn5 transposon mutagenesis, restriction endonuclease analysis, and dna-dna hybridization, two dna fragments, a 9.5-kb kpni fragment and a 13.5-kb hindiii fragment (hki), were isolated. hki contained ek1, the kpni fragment, as a su ...	1989	2549012
genetics of hydrogenase from aerobic lithoautotrophic bacteria.	aerobic facultatively autotrophic hydrogen bacteria are distinguished on the basis of their hydrogen-oxidizing enzyme system (hox). the major group, represented by paracoccus denitrificans and pseudomonas facilis, contains a membrane-bound, electron transport-coupled protein. species of nocardia are characterized by the possession of a cytoplasmic nad-dependent hydrogenase. both enzymes are present in strains of alcaligenes. all hydrogenases from lithoautotrophs are h2-consuming nickel-iron-sulf ...	1986	3089306
conjugal transfer of hydrogen-oxidizing ability of alcaligenes hydrogenophilus to pseudomonas oxalaticus.	conjugal transfer of hydrogen-oxidizing ability (hox) of the hydrogen bacterium alcaligenes hydrogenophilus was examined. intraspecific cross of plasmid phg21-a that encodes hydrogenases that mediate hydrogen oxidation was most frequent at 25 c; the optimal temperature for growth was 30 c. the plasmid could be transferred from a. hydrogenophilus to pseudomonas oxalaticus ox1 and ox4, and the resulting strains gained the capacity for autotrophic growth with h2 and co2. plasmid phg21-a was maintai ...	1986	3521600
isolation of hydrogen-oxidation gene from alcaligenes hydrogenophilus and its expression in pseudomonas oxalaticus.	a gene bank of a megaplasmid encoding the hydrogen-oxidizing enzyme system (hox) in alcaligenes hydrogenophilus was constructed using a broad host range cosmid vector pvk102, and established in escherichia coli. hybrid cosmids containing hox genes were identified by transferring the bank into pseudomonas oxalaticus ox1 and screening colonies for the ability of h2-dependent autotrophic growth. about 800 colonies were formed under autotrophic conditions. one of the hox+ transconjugants was isolate ...	1986	3521601
expression of hydrogenase in alcaligenes spp. is altered by interspecific plasmid exchange.	alcaligenes hydrogenophilus was found to contain a soluble and a particulate hydrogenase whose control and structure differed in part from that in alcaligenes eutrophus. one of at least two plasmids indigenous to a. hydrogenophilus determines hydrogenase genes (hox). the interspecific exchange of hox-encoding plasmids generated transconjugants which expressed the structural and regulatory hox phenotype of the donor.	1984	6370961
[conjugal transfer of chemolithoautotrophically growing ability from hydrogen-oxidizing bacterium alcaligenes hydrogenophilus to useful material-producing bacteria].	the plasmid genes encoding the ability to grow chemolithoautotrophically with h2 and co2 from a h2-oxidizing bacterium alcaligenes hydrogenophilus were in vivo cloned using the broad host range inc p1 r-plasmid r68.45. the genes of h2-oxidation enzymes were expressed by a novel alternative sigma 54-like factor encoded on the chromosome. the sigma 54-like factor was also in vivo cloned using r68.45. both r68.45-primes, one carrying plasmid genes and the other carrying chromosome genes, were in vi ...	1994	8169770
a hydrogen-sensing system in transcriptional regulation of hydrogenase gene expression in alcaligenes species.	heterologous complementation studies using alcaligenes eutrophus h16 as a recipient identified a hydrogenase-specific regulatory dna region on megaplasmid phg21-a of the related species alcaligenes hydrogenophilus. nucleotide sequence analysis revealed four open reading frames on the subcloned dna, designated hoxa, hoxb, hoxc, and hoxj. the product of hoxa is homologous to a transcriptional activator of the family of two-component regulatory systems present in a number of h2-oxidizing bacteria. ...	1997	9045826
cloning and sequence analysis of the poly (3-hydroxyalkanoic acid)-synthesis genes of pseudomonas acidophila.	pseudomonas acidophila can grow with co2 as a sole carbon source by the possession of a recombinant plasmid that clones genes that confer chemolithoautotrophic growth ability derived from the h2-oxidizing bacterium alcaligenes hydrogenophilus. h2-oxidizing bacteria produce poly(3-hydroxybutyric acid) (phb) from co2, but recombinant p. acidophila can produce the more useful biopolymer poly(3-hydroxyalkanoic acid) (pha). in this study, the pha genes of p. acidophila were cloned and a sequence anal ...	1998	9627389
a novel multicomponent regulatory system mediates h2 sensing in alcaligenes eutrophus.	oxidation of molecular hydrogen catalyzed by [nife] hydrogenases is a widespread mechanism of energy generation among prokaryotes. biosynthesis of the h2-oxidizing enzymes is a complex process subject to positive control by h2 and negative control by organic energy sources. in this report we describe a novel signal transduction system regulating hydrogenase gene (hox) expression in the proteobacterium alcaligenes eutrophus. this multicomponent system consists of the proteins hoxb, hoxc, hoxj*, a ...	1998	9770510
a megaplasmid-borne anaerobic ribonucleotide reductase in alcaligenes eutrophus h16.	the conjugative 450-kb megaplasmid phg1 is essential for the anaerobic growth of alcaligenes eutrophus h16 in the presence of nitrate as the terminal electron acceptor. we identified two megaplasmid-borne genes (nrdd and nrdg) which are indispensable under these conditions. sequence alignment identified significant similarity of the 76.2-kda gene product nrdd and the 30.9-kda gene product nrdg with anaerobic class iii ribonucleotide reductases and their corresponding activases. deletion of nrdd ...	1999	10438763
identification and characterization of hupt, a gene involved in negative regulation of hydrogen oxidation in bradyrhizobium japonicum.	the bradyrhizobium japonicum hupt gene was sequenced, and its gene product was found to be homologous to ntrb-like histidine kinases. a hupt mutant expresses higher levels of hydrogenase activity than the wild-type strain under hydrogenase-inducing conditions (i.e., microaerobiosis plus hydrogen, or symbiosis), whereas in noninduced hupt cells, hupsl expression is derepressed but does not lead to hydrogenase activity. we conclude that hupt is involved in the repression of hupsl synthesis at the ...	1999	10438783
the h(2) sensor of ralstonia eutropha is a member of the subclass of regulatory [nife] hydrogenases.	two energy-generating hydrogenases enable the aerobic hydrogen bacterium ralstonia eutropha (formerly alcaligenes eutrophus) to use molecular hydrogen as the sole energy source. the complex synthesis of the nickel-iron-containing enzymes has to be efficiently regulated in response to h(2), which is available in low amounts in aerobic environments. h(2) sensing in r. eutropha is achieved by a hydrogenase-like protein which controls the hydrogenase gene expression in concert with a two-component r ...	2000	10781538
characterization of the hydrogen-deuterium exchange activities of the energy-transducing hupsl hydrogenase and h(2)-signaling hupuv hydrogenase in rhodobacter capsulatus.	rhodobacter capsulatus synthesizes two homologous protein complexes capable of activating molecular h(2), a membrane-bound [nife] hydrogenase (hupsl) linked to the respiratory chain, and an h(2) sensor encoded by the hupuv genes. the activities of hydrogen-deuterium (h-d) exchange catalyzed by the hupsl-encoded and the hupuv-encoded enzymes in the presence of d(2) and h(2)o were studied comparatively. whereas hupsl is in the membranes, hupuv activity was localized in the soluble cytoplasmic frac ...	2000	11029418
biosynthesis of poly(3-hydroxyalkanoic acid) copolymer from co(inf2) in pseudomonas acidophila through introduction of the dna fragment responsible for chemolithoautotrophic growth of alcaligenes hydrogenophilus.	pseudomonas acidophila is a bacterial strain producing a poly(3-hydroxyalkanoic acid) (pha) copolymer from low-molecular-weight organic compounds such as formate and acetate. the genes responsible for pha production were cloned in cosmid pik7 containing a 14.8-kb hindiii fragment of p. acidophila dna. with the aim of developing a means of producing a pha copolymer from co(inf2), cosmid pik7 was introduced into a polymer-negative mutant of the chemolithoautotrophic bacterium alcaligenes eutrophus ...	1996	16535252
optimization of biomass productivity and substrate utility of a hydrogen bacterium, alcaligenes hydrogenophilus.	optimizations of cell productivity and utility of gaseous substrates were investigated for alcaligenes hydrogenophilus. theoretical analyses for the cell productivity and substrate utility were discussed in the light of experimental data. it was proved from the theoretical analyses that the maximum cell productivity was at the following conditions: d = 0.21 h(-1), r(1) ( = h(2) partial pressure/o(2) partial pressure) = 2.25 and r(2) ( =co(2) partial pressure/o(2) partial pressure) = 0.09, i.e., ...	1982	18546410
reverse-transcriptase quantitative pcr method to detect uptake of hydrogen produced from cyanobacteria by alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium.	hydrogen-oxidising bacteria play a key ecological role in a variety of habitats including the rhizosphere and hot springs. to investigate the possibly of interspecies hydrogen exchange between cyanobacteria and hydrogen-oxidising bacteria, we developed a sensitive and reliable reverse-transcriptase qpcr assay for up-regulation of the hups gene in the knallgas bacterium alcaligenes hydrogenophilus dsm2625. the assay detected up-regulation of the gene at initial hydrogen concentrations as low as 0 ...	2011	21617984
metagenomic evidence for h(2) oxidation and h(2) production by serpentinite-hosted subsurface microbial communities.	ultramafic rocks in the earth's mantle represent a tremendous reservoir of carbon and reducing power. upon tectonic uplift and exposure to fluid flow, serpentinization of these materials generates copious energy, sustains abiogenic synthesis of organic molecules, and releases hydrogen gas (h(2)). in order to assess the potential for microbial h(2) utilization fueled by serpentinization, we conducted metagenomic surveys of a marine serpentinite-hosted hydrothermal chimney (at the lost city hydrot ...	2011	22232619
metagenomic evidence for h(2) oxidation and h(2) production by serpentinite-hosted subsurface microbial communities.	ultramafic rocks in the earth's mantle represent a tremendous reservoir of carbon and reducing power. upon tectonic uplift and exposure to fluid flow, serpentinization of these materials generates copious energy, sustains abiogenic synthesis of organic molecules, and releases hydrogen gas (h(2)). in order to assess the potential for microbial h(2) utilization fueled by serpentinization, we conducted metagenomic surveys of a marine serpentinite-hosted hydrothermal chimney (at the lost city hydrot ...	2011	22232619
reversible oxygen-tolerant hydrogenase carried by free-living n2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat.	hydrogen production by microorganisms is often described as a promising sustainable and clean energy source, but still faces several obstacles, which prevent practical application. among them, oxygen sensitivity of hydrogenases represents one of the major limitations hampering the biotechnological implementation of photobiological production processes. here, we describe a hierarchical biodiversity-based approach, including a chemochromic screening of hydrogenase activity of hundreds of bacterial ...	2012	23233392
expression and regulation of the ery operon of brucella melitensis in human trophoblast cells.	brucellosis is primarily a disease of domestic animals in which the bacteria localizes to fetal tissues such as embryonic trophoblast cells and fluids containing erythritol, which stimulates brucella spp. growth. the utilization of erythritol is a characteristic of the genus brucella. the ery operon contains four genes (erya, eryb, eryc and eryd) for the utilization of erythritol, and plays a major role in the survival and multiplication of brucella spp. the objective of the present study was to ...	2016	27698777