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[biochemical differentiation between n. sicca and n. perflava (author's transl)].neisseria sicca forms an iodine-positive metabolic product when grown on trypticase soy agar without the addition of sucrose. this reaction which does not occur with n. perflava may be useful for differentiating between the two species.197551550
neisseria sicca endocarditis: report of a case and review of the literature.a rare case of bacterial endocarditis caused by neisseria sicca is reported. review of the literature revealed only five other cases where sufficient data existed to confirm this particular organism as the etiological agent of bacterial endocarditis.1978253721
interactions between neisseria sicca and viridin b, a bacteriocin produced by streptococcus mitis.viridin b, a bacteriocin produced by streptococcus mitis (mitior), is bactericidal to neisseria sicca. oxygen consumption by actively growing n. sicca cultures ceased immediately upon exposure to viridin b. adenosine triphosphate production was slightly enhanced within 1 h of exposure to the bacteriocin but was subsequently repressed. the uptake and incorporation of glucose was prevented in the presence of viridin b. the bacteriocin also blocked uptake of an amino acid mixture in chloramphenicol ...1978263887
comparison of three methods for identification of pathogenic neisseria species.a radiometric procedure was compared with the minitek and cystine trypticase agar sugar degradation methods for identification of 113 neisseria species (58 neisseria meningitidis, 51 neisseria gonorrhoeae, 2 neisseria lactamica, 2 neisseria sicca). identification of meningococci and gonococci was confirmed by agglutination and fluorescent antibody techniques, respectively. the minitek method identified 97% of meningococci, 92% of gonococci, and 100% of other neisseria after 4 h of incubation. th ...1979383747
prosthetic valve endocarditis due to neisseria sicca. a case report. 1979489043
selective isolation of neisseria sicca from the human oral cavity on eosin methylene blue agar.strains of neisseria sicca and n. mucosa, but not n. perflava, n. subflava, n. flava, or n. flavescens were found to grow on eosin methylene blue agar. the distribution of n. sicca on the tongue dorsum, the gingival crevice area, and the coronal surfaces of teeth of humans was determined using this medium. n. sicca averaged about 5% of the total cultivable organisms of the tongues of 14 subjects examined, but it was present in only trace quantities in dental plaque on the coronal surfaces of tee ...1976773308
ultrastructural and biochemical alterations effected by viridin b, a bacterocin of alpha-hemolytic streptococci.viridin b, a bacteriocin produced by streptococcus mitis strain 42885, was shown previously to exert a bactericidal effect against a neisseria sicca strain but only a bacteriostatic effect against a coagulase-negative staphylococcus. in the present report, this dual effect was defined further. viridin b caused the appearance of mesosomes in both indicator strains early in the course of exposure. whereas mesosomes were the only noticeable alterations in the staphylococcal strain at all times of e ...1976965095
envelope proteins in neisseria.the proteins of the cell envelope of neisseria sicca strain atcc 9913 have been examined by sds - polyacrylamide gel electrophoresis. some 20 proteins were resolved from the total envelope fraction, and nearly all of these were found to be localized in the outer membrane. preparations of the "free-endotoxin" fraction differ from the outer membrane only in lacking a few minor proteins. the behavior of several of the envelope proteins on electrophoresis can be modified by changing the temperature ...19751104115
two-dimensional sds-polyacrylamide gel electrophoresis of heat-modifiable outer-membrane proteins.an examination has been made of the effect which temperature of solubilization has upon the subsequent migration in sds-polyacrylamide gel electrophoresis of proteins from the cell envelopes of escherichia coli k12 and neisseria sicca atcc 9913. conventional electrophoresis in tubes revealed substantial differences in the staining patterns of gels, depending upon whether the envelope samples were solubilized at 37 degrees c or 100 degrees c; in the case of n. sicca at least 6 of 13 discernible b ...19761252992
sucrose-mediated giant cell formation in the genus neisseria.growth of neisseria perflava, neisseria cinerea, and neisseria sicca strain kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. giant cells appeared only under growth conditions and did not lyse upon transfer to medium lacking sucrose or upon resuspension in hypotonic media. reversion of giant to normal cells occurred when giant cells ...19761253000
viridins, bacteriocins of alpha-hemolytic streptococci: isolation, characterization, and partial purification.bacteriocin-like activities were detected in 78% of 120 alpha-hemolytic streptococcal isolates. inhibitory substances from three such isolates (one streptococcus sanguis strain and two s. mitis strains) were investigated further and termed viridins (a, b, and c). the viridins were unique among bacteriocins of gram-positive bacteria in that they inhibited many gram-negative bacteria in addition to inhibition of a variety of gram-positive organisms. viridins were obtained in a cell-free state only ...19761259394
neisseria sicca endocarditis complicating pregnancy. a case report.neisseria sicca, a commensal organism of the oropharynx, has rarely been implicated as a pathogen responsible for causing bacterial endocarditis. a pregnant woman developed n sicca endocarditis at 32 weeks' gestation. although there was an initial delay in diagnosis and therapy, the maternal and fetal outcomes were excellent.19921507196
infective endocarditis due to neisseria sicca and associated with intravenous drug abuse.intravenous drug abusers are subject to infective endocarditis from unusual pathogens, including the saprophytic species of neisseria, sometimes transmitted by needles contaminated with oral secretions. we have recently encountered such a case, in which a 37-year-old man with vegetations on the anterior leaflet of the tricuspid valve had blood cultures positive for n sicca. a history of intravenous drug abuse using needles contaminated with oral secretions should alert clinicians to the possibil ...19921523457
neisseria sicca endocarditis with embolic phenomena.two patients with poor oral hygiene developed neisseria sicca endocarditis, one after probable intravenous drug abuse and staphylococcus aureus endocarditis and the other after a periodontal surgical procedure. both experienced significant embolic phenomena and both required 6 or more weeks of intravenous antibiotic therapy. the diagnosis of n. sicca endocarditis must be considered when this organism is isolated from blood cultures in patients with emboli.19921572142
nonsporulating bacterial species contain dna sequences homologous to the bacillus spore-specific c-protein gene.genes for small, acid-soluble spore proteins (sasps) are ubiquitous among the spore-forming bacteria and are expressed only during sporulation. although they perform the function of amino acid storage in spores, the members of the sasp-c multigene family probably serve additional functions, so that similar sequences might be present in non-spore-formers. using the sasp-c gene (ssp-c) as a hybridization probe, restriction digests of whole genomic dna from seven nonsporulating bacterial species we ...19911848527
bacterial arthritis.in this review of the 1990 septic arthritis literature, we revisit synovial fluid leukocytosis, examine the utility of synovial fluid glucose and protein measurements, and look at the levels of two cytokines, tumor necrosis factor and interleukin-1, in infected joint fluids. we see the many faces of gonococcal arthritis and the ravages of septic arthritis when the host has rheumatoid arthritis. should we recommend antibiotic prophylaxis for the rheumatoid patient with a prosthetic joint who is u ...19911911055
expression of carbonic anhydrase in neisseriae and other heterotrophic bacteria.a diverse range of heterotrophic bacteria was screened for the presence of carbonic anhydrase (ca) activity, sensitivity to inhibition of growth by acetazolamide (ca inhibitor), and the presence of protein binding monospecific antibody prepared against purified neisseria sicca ca. ca activity was demonstrated only in strains of n. sicca and n. gonorrhoeae. however, all neisseria strains, including various isolates of n. meningitidis and n. lactamica, were sensitive to acetazolamide, when grown i ...19902111405
septic olecranon bursitis caused by neisseria sicca.a man with rheumatoid arthritis developed a septic olecranon bursitis due to neisseria sicca. this case confirms the potential pathogenicity of neisseria sicca and emphasizes that positive cultures for this organism should not be readily dismissed.19902290170
comparative adhesion of seven species of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis to fibrin-platelet clots in vitro.the adhesion to fibrin-platelet clots in vitro of 21 strains of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis (sabe) was measured. the species, in order of greatest adhesion, were streptococcus faecalis, streptococcus mutans, streptococcus milleri, streptococcus sanguis, dextran-positive streptococcus mitior, dextran-negative streptococcus mitior and streptococcus salivarius. individual strains within species, however, cannot be assumed to be representati ...19862422150
identification of epitopes recognized by monoclonal antibodies sm1 and sm2 which react with all pili of neisseria gonorrhoeae but which differentiate between two structural classes of pili expressed by neisseria meningitidis and the distribution of their encoding sequences in the genomes of neisseria spp.the pili expressed by all isolates of neisseria gonorrhoeae react with two monoclonal antibodies, sm1 and sm2. in contrast, although many isolates of neisseria meningitidis also express pili (class i) which react with antibodies sm1 and sm2, a proportion express pili (class ii) which fail to react. in order to define the epitopes recognized by these antibodies, a series of overlapping peptides corresponding to the amino acid sequence of conserved regions of gonococcal pili have been synthesized. ...19892483993
comparison of the quadferm+ 2-hr identification system with conventional carbohydrate degradation tests for confirmatory identification of neisseria gonorrhoeae.the quadferm+ (analytab products, plainview, ny) rapid identification system was evaluated for its ability to identify correctly neisseria gonorhoeae isolates from specimens obtained at a sexually transmitted disease clinic. one hundred eighty-five isolates (115 n. gonorrhoeae, 45 neisseria meningitidis, 16 neisseria species, and nine branhamella catarrhalis; fresh isolates, frozen stock cultures, and cultures referred from local health agencies) were tested with the quadferm+ system and convent ...19892500720
respiratory symptoms due to branhamella catarrhalis and other neisseria species infections--response to erythromycin therapy.neisseria microorganisms (neisseria lactamica, neisseria sicca, and neisseria mucosa) are regarded as normal respiratory commensals. branhamella catarrhalis (formerly neisseria catarrhalis) has also been regarded as a normal respiratory commensal, but reports indicate that it can be pathogenic. the role of neisseria spp was studied in 160 patients with chest infections and symptoms and signs of obstructive respiratory disease. group i patients (n = 140) had a history of asthma, bronchitis, and e ...19892509070
neisseria sicca pneumonia and bronchiectasis.neisseria sicca, although considered a harmless saprophyte, has been recognised as an etiologic agent in three cases of pneumonitis, and rare cases of endocarditis, meningitis, and osteomyelitis, particularly in immunocompromised hosts. we report the case of a 76-year-old man with a community-acquired pneumonia, in whom both sputum samples and bronchial secretions obtained with bronchoscopic protected catheter brush grew pure culture of n. sicca with abundant polymorphonuclear neutrophils. drama ...19892776875
gardnerella vaginalis bacteremia from pulmonary abscess in a male alcohol abuser.a case of gardnerella vaginalis bacteremia is reported. this bacteremia occurred in a male alcohol abuser who developed definite signs of pulmonary abscess and empyema. streptococcus milleri grew from another blood culture, but gardnerella vaginalis was also isolated from a bronchoscopic aspirate and pleural drainage sample as part of mixed flora containing anaerobes, streptococcus species, neisseria sicca, and a haemophilus sp. we discuss the possible pathogenic character of g. vaginalis outsid ...19892787333
fatal frontal sinusitis due to neisseria sicca and eubacterium lentum.infectious sinusitis may on occasion be associated with meningitis, subdural empyema, epidural empyema, brain abscess, or osteomyelitis. we report a 29-year-old male patient with frontal sinusitis who developed all of these intracranial complications due to two previously unreported causative organisms, neisseria sicca and eubacterium lentum. the fulminant and fatal course resulting from locally invasive disease underscores the importance of early diagnosis and proper treatment of these complica ...19862873255
a multi-resistance plasmid isolated from commensal neisseria species is closely related to the enterobacterial plasmid rsf1010.pfm739, an r plasmid from neisseria sicca that encodes penicillin, streptomycin and sulphonamide resistance, and the enterobacterial incq(p-4) plasmid rsf1010, which encodes streptomycin and sulphonamide resistance, were incompatible, and were mobilized by the same conjugative plasmids. restriction mapping confirmed a high degree of similarity between both r plasmids; pfm739 carried dna fragments corresponding to the known replication and resistance regions of rsf1010. pfm739 also carried an ext ...19863025338
bartholin's gland abscess caused by neisseria sicca.neisseria sicca has been reported to cause bacterial meningitis, endocarditis, pneumonia, and spondylitis. surgical drainage from a bartholin's gland abscess contained n. sicca in pure culture. neisseria species recovered from cerebrospinal fluid and gynecologic specimens must be carefully examined to avoid misidentification as meningococci or gonococci.19883170716
microcalorimetric determination of the kinetics of substrate utilisation by non-growing suspensions of neisseria sicca.the metabolism of various substrates by non-growing suspensions of neisseria sicca was investigated by a flow-microcalorimetric technique. substrate utilisation showed michaelis kinetics allowing determination of saturation constants (km) and maximum specific rates of substrate utilisation (vmax). pyruvate, lactate, a number of tricarboxylic acid cycle intermediates, and amino acids (aspartate, glutamate and proline) were rapidly metabolised [vmax 5-35 mumol (g dry wt cells)-1 min-1]; km values ...19883173127
campylobacter pyloridis: a new organism to explain an old problem? 19873309916
factors affecting the survival of neisseria sicca.cultures of neisseria sicca incubated at 37 degrees c died rapidly (within 36 h) after growth ceased. re-suspending cells in a brain heart infusion broth and storing at 4 degrees c greatly reduced the rate of decline in viability (decimal reduction time 6 days). an important factor in maintaining viability was apparently the presence of external energy source(s). survival comparable to that in broth was obtained by incubation in ringer's solution with pyruvate plus glucose (but not with pyruvate ...19883374398
production of bacteriocin-like substances by human oral streptococci.a sample of human streptococci (mainly streptococcus mutans species) from dental plaques was examined in order to evaluate the production frequency and activity spectrum of bacteriocin-like substances (mutacins). 89% of the 55 streptococcus mutans strains produced substances with a wide spectrum of antibacterial activity. the bacteriocins produced showed a marked inhibitory activity against gram-positive bacteria. among the gram-negative species tested, only neisseria sicca was inhibited by 25% ...19873695984
evaluation of a rapid identification method for neisseria spp.a comparison was made of carbohydrate degradation reactions of neisseria gonorrhoeae, neisseria meningitidis, neisseria lactamica, neisseria mucosa, neisseria sicca, neisseria subflava, and branhamella catarrhalis in a rapid (0.5- to 1-h) identification micromethod (rim-n kit; austin biological laboratories, inc., austin, tex.) and in a serum-free agar slanted medium (72 h). reactions after 1 h in the rim-n system agreed completely with those after 72 h in the conventional system.19853891778
neisseria sicca endocarditis in intravenous drug abusers. 19854013255
neisseria sicca pneumonia. report of two cases and review of the literature.neisseria sicca is considered part of the normal flora of the oropharynx. although usually believed to be a harmless saprophyte, it has rarely been recognized as an etiologic agent in human disease, particularly in immunodeficient hosts. two immunodeficient women are described in whom n. sicca pneumonia of the left lower lobe developed. one patient was 30 years old and had just completed a full-term pregnancy, and the other patient was 73 years of age and was receiving prednisone for bullous pem ...19854014264
effect of saccharin on growth and acid production of glucose-grown pathogenic and oral bacteria.growth and acid production of glucose-grown gram-positive and gram-negative rods as well as cocci from the human oral cavity were studied in the presence of 0.02 to 20.00 mg/ml sodium saccharin. all gram-positive rods, i.e. actinomyces viscosus, lactobacillus acidophilus, bacillus subtilis and corynebacterium diphtheriae, and gram-positive cocci, i.e. streptococcus spp, staphylococcus aureus and micrococcus luteus, were significantly inhibited by saccharin, especially at the higher concentration ...19854033457
further studies on thymidine kinase: distribution pattern of the enzyme in bacteria.various micro-organisms (131 strains of 73 species) were studied for their ability to produce thymidine kinase (tk; ec 2.7.1.21). taking the specific tk activity of escherichia coli k12 [specific activity of sonicated cell extracts 95-194 pmol min-1 (mg protein)-1] as 100%, the test organisms had the following relative specific tk activities. in the gram-positive cocci, staphylococcus aureus (21-84%) showed higher activity than staph. epidermidis (1-20%) and streptococcus (1-7%) except for one s ...19854093763
a comparison of the fine structure of micrococcus diversus, neisseria catarrhalis and neisseria sicca. 19704194101
structural investigations on a cell-wall lipopolysaccharide from neisseria sicca. 19714322745
galactosamine polymer isolated from the cell wall of neisseria sicca. 19724623881
carbonic anhydrase from neisseria sicca, strain 6021. i. bacterial growth and purification of the enzyme. 19724627452
carbonic anhydrase from neisseria sicca, strain 6021. ii. properties of the purified enzyme. 19724627453
aerosols of mycoplasmas, l forms, and bacteria: comparison of particle size, viability, and lethality of ultraviolet radiation.aerosols of microorganisms were tested for particle size by use of an andersen sampler. mycoplasma aerosols had an average count median diameter (cmd) of 2.1 +/- 0.5 mu. staphylococcus aureus l forms gave an average cmd of 4.6 +/- 1.7 mu; the diphtheroid l form, a cmd of 3.4 +/- 0.3 mu. escherichia coli had a cmd of 5.4 +/- 2.5 mu; neisseria sicca, 3.3 +/- 0.5 mu; n. meningitidis, 3.4 +/- 0.2 mu. s. aureus atcc 6538, the parent strain of the l form, yielded a cmd of 3.9 +/- 1.2 mu. candida albic ...19684865901
antibacterial action of essential oils of artemisia as an ecological factor. i. antibacterial action of the volatile oils of artemisia tridentata and artemisia nova on aerobic bacteria.bacterial response to increasing amounts of the volatile oils varies significantly according to species of bacteria tested. among the four species examined, escherichia coli was the most resistant to the oils, followed by neisseria sicca, bacillus subtilis, and staphylococcus aureus. the oils of artemisia tridentata seem to have the same degree of antibacterial action as oils obtained from a. nova.19674963443
reactivity of two gonococcal antigens in an automated microhemagglutination procedure.in studies of several hundred sera, a passive-hemagglutination technique with soluble antigen of sonically treated gonococci as the sensitizing material for tanned erythrocytes and neisseria sicca sonically treated material as an absorbent detected gonococcal antibodies in 77% of males and 88% of females infected with uncomplicated gonorrhea. however, 6% of the sera from individuals in celibate religious orders and 18% of the sera from a group of females having cervical cultures negative for gon ...19704992656
influence of cultural conditions on the lipopolysaccharide composition of neisseria sicca. 19715089334
[purulent meningitis and sepsis with demonstration of neisseria sicca]. 19665941952
the significance of nongonococcal, nonmeningococcal neisseria isolates from blood cultures.nongonococcal, nonmeningococcal neisseriae are part of the normal respiratory flora and infrequently cause disease. these organisms include neisseria lactamica, neisseria mucosa, neisseria sicca, neisseria flavescens, neisseria subflava , neisseria perflava , neisseria flava , and branhamella catarrhalis (previously classified as neisseria catarrhalis). blood cultures positive for these bacteria have been associated with serious infections, including endocarditis, septicemia, and meningitis. in ...19846374834
diagnostic value of interactions between members of the family neisseriaceae and lectins.the lectin slide agglutination test for neisseria gonorrhoeae has been modified and improved. results show that wheat germ agglutinin and soybean lectin agglutinate 100% (193 of 193 tested) of clinical isolates of n. gonorrhoeae. lectin-reactive meningococci can be readily identified by the hydrolysis of gamma-glutamyl-beta-naphthylamide. branhamella catarrhalis, neisseria lactamica, neisseria sicca, neisseria subflava, neisseria perflava, and meningococcal serogroups a, b, c, x, y, and z do not ...19846546936
localization of carbonic anhydrase in the cytoplasmic membrane of neisseria sicca (strain 19).the carbonic anhydrase activity and the growth of neisseria sicca 19 were inhibited by the sulfonamide acetazolamide (10(-5) m). such inhibition was completely overcome by the addition of exogenous bicarbonate. some carbonic anhydrase activity associated with the membranous envelope fraction of the cell was released when cells were broken by sonic treatment but not during cell breakage by high-pressure extrusion. after the selective solubilization (4 degrees c) of the inner membrane of envelopes ...19816783286
neisseria sicca osteomyelitis.neisseria sicca was identified as the cause of vertebral osteomyelitis in a male patient who had previously suffered a nonpenetrating, traumatic back injury. the identifying characteristics and antimicrobial susceptibility patterns are presented for this rare human pathogen, which heretofore has not been reported as a cause of infection localized to bone.19827130376
viability of six species of normal oropharyngeal bacteria after exposure to cigarette smoke in vitro.bacteria in culture medium at 37 degrees c were subjected to eight puffs of smoke from one cigarette, and viable cell counts were done at intervals ranging from 0 min to 3 h after exposure to smoke. the cigarette contained 23.0 mg of tar and 1.4 mg of nicotine. each 25.0 ml puff of smoke from a mechanical smoking apparatus was passed over a 1.0 ml suspension of bacteria dispersed over a 25 cm2 surface in a sterile flask. filtered air was in contact with the suspension between puffs, and during t ...19817339446
neisseria sicca pneumonia. 19807351135
bacteria in human mouths involved in the production and utilization of hydrogen peroxide.earlier studies have demonstrated that pure cultures of oral streptococci produce hydrogen peroxide but none has found any free peroxide in dental plaque or salivary sediment despite streptococci being major components of their mixed bacterial populations. the absence of peroxide in plaque and sediment could be due to the dominance of its destruction over its formation by bacterial constituents. to identify which of the oral bacteria might be involved in such a possibility, pure cultures of 27 d ...19957487577
[changed etiopathogenic and clinical features of infective endocarditis].the authors report their experience on etiological and clinical aspects of infective endocarditis (ie). a series of 182 consecutive patients, including 83 cases of medical ie, 73 cases of ie in intravenous drug abusers (da), 22 cases of ie on late prosthetic valves and 4 cases of ie on early prosthetic valves were evaluated since 1976. medical ie occurred frequently in the elderly patients and in most of the cases (80%) involved natural valves with underlying abnormalities, either rheumatic (42% ...19947634278
investigations into the molecular basis of meningococcal toxicity for human endothelial and epithelial cells: the synergistic effect of lps and pili.using human umbilical vein endothelial cells as an in vitro model of toxicity, it was found that neisseria meningitidis, neisseria gonorrhoeae, neisseria lactamica and neisseria sicca caused damage to these cells, in contrast to the lack of cytotoxicity exhibited by haemophilus influenzae type b. n. meningitidis was also found to be toxic for human epithelial cells. the major toxic factor of n. meningitidis was found to be a heat-stable component of outer membrane vesicles, and could be inhibite ...19957643745
cerebrospinal fluid shunt infection by neisseria sicca.neisseria sicca is considered to be a nonpathogenic oral saprophyte. presented here is an unusual case of cerebrospinal fluid (csf) shunt infection by n. sicca. although medical management of the common community-acquired meningitides, including infection by neisseria meningitidis, is often successful in patients with csf shunts, removal and replacement of the infected shunt was necessary in this case.19947803309
neisseria sicca peritonitis in a patient maintained on chronic peritoneal dialysis.neisseria sicca, previously classified as a "nonpathogenic" organism, has now been recognized as a cause of many infections, including endocarditis and meningitis. however, it has not been reported as a cause of peritonitis. we present a case of documented n. sicca peritonitis immediately following an episode of staphylococcus aureus peritonitis in a pediatric chronic peritoneal dialysis (cpd) patient. n. sicca should be considered as a possible pathogen in cpd-associated peritonitis.19947819012
native-valve endocarditis due to neisseria sicca. 19948038335
native-valve endocarditis due to neisseria sicca: case report and review.many species of the neisseria, gram-negative diplococci that are frequent respiratory commensals in humans, have been regarded as being nonpathogenic or as causing disease in only immunocompromised hosts; in contrast, gram-negative diplococci such as neisseria meningitidis and neisseria gonorrhoeae are known pathogens. we report a case in which neisseria sicca was the cause of serious infection (with catastrophic consequences) in an immunocompetent patient and review the world literature on endo ...19938507758
biodegradation of cellulose acetate by neisseria sicca.bacteria capable of assimilating cellulose acetate, strains sb and sc, were isolated from soil on a medium containing cellulose acetate as a carbon source, and identified as neisseria sicca. both strains degraded cellulose acetate membrane filters (degree of substitution, ds, mixture of 2.8 and 2.0) and textiles (ds, 2.34) in a medium containing cellulose acetate (ds, 2.34) or its oligomer, but were not able to degrade these materials in a medium containing cellobiose octaacetate. biodegradation ...19968987659
[neisseria sicca endocarditis disclosed by multiple organ failure syndrome].the authors report a case of neisseria sicca endocarditis presenting with multiple organ failure in a 33 year old intravenous drug user. the diagnosis was confirmed by transthoracic and transoesophageal echocardiography showing vegetations on the aortic valve and three blood cultures positive for neisseria sicca. massive aortic regurgitation occurred on the 4th day. the patient died of complications of intracerebral haematomas before surgical intervention. contrary to pathogenic neisseria gonorr ...19969137737
endocarditis due to neisseria sicca: report of one case.many species of the neisseria, which are respiratory commensals in humans, have been regarded as being nonpathogenic or as causing disease in only immunocompromised hosts. we report a case in which neisseria sicca was the cause of infective endocarditis in a child with a ventricular septal defect and review the literature on endocarditis due to n. sicca infection. most of these patients had an underlying heart disease. dental caries and poor oral hygiene may be two factors that predispose patien ...19979230543
sequence diversity, predicted two-dimensional protein structure, and epitope mapping of neisserial opa proteins.the sequence diversity of 45 opa outer membrane proteins from neisseria meningitidis, neisseria gonorrhoeae, neisseria sicca, and neisseria flava indicates that horizontal genetic exchange of opa alleles has been rare between these species. a two-dimensional structural model containing four surface-exposed loops was constructed based on rules derived from porin crystal structure and on conservation of sequence homology within transmembrane beta-strands. the minimal continuous epitopes recognized ...19989495774
bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds.this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ...19989501433
ciprofloxacin versus cefuroxime axetil in the treatment of adult patients with acute bacterial sinusitis.this study compared the use and efficacy of ciprofloxacin to cefuroxime axetil for adult patients with acute bacterial sinusitis.19989511113
immunoreactivity of five monoclonal antibodies against the 37-kilodalton common cell wall protein (psaa) of streptococcus pneumoniae.five monoclonal antibodies (mabs) were produced against the streptococcus pneumoniae pneumococcal surface adhesin a (psaa) 37-kda common cell wall protein. these antibodies were used in a dot immunoblot and western blot study of clinical isolates of s. pneumoniae to detect the presence of the protein. by both assays, the mabs reacted with clinical isolates representing the 23 type-specific serotypes present in the licensed pneumococcal polysaccharide vaccine. western blot analysis confirmed the ...19989521144
septic arthritis caused by neisseria sicca. 19989558202
the regulated outer membrane protein omp21 from comamonas acidovorans is identified as a member of a new family of eight-stranded beta-sheet proteins by its sequence and properties.omp21, a minor outer membrane protein of the soil bacterium comamonas acidovorans, was purified from a spontaneous mutant lacking a surface layer and long-chain lipopolysaccharide. omp21 synthesis is enhanced by oxygen depletion, and the protein has a variable electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis due to its heat-modifiable behavior. the structural gene omp21 encodes a precursor of 204 amino acids with a putative signal peptide of 21 amino acids. m ...19989683466
differentiation between mycobacterium tuberculosis and mycobacterium avium by amplification of the 16s-23s ribosomal dna spacer.differentiation between mycobacterium tuberculosis and m. avium is helpful for the treatment of disseminated mycobacterial infection in aids patients. this can traditionally be done by time-consuming biochemical tests or with accuprobe. previously, pcr restriction enzyme analysis (pcr-rea) of the 16s-23s rrna gene spacer was shown to be able to identify a limited number of strains of mycobacterium. in this study the method was improved by using more specific primers and was tested with 50 clinic ...19989705362
evaluation of amplicor neisseria gonorrhoeae pcr using cppb nested pcr and 16s rrna pcr.certain strains of neisseria subflava and neisseria cinerea are known to produce false-positive results with the amplicor neisseria gonorrhoeae pcr (roche diagnostic systems, branchburg, n.j.). the analytical sensitivity and analytical specificity of three pcr tests were assessed with 3 geographically diverse n. gonorrhoeae strains and 30 non-n. gonorrhoeae neisseria spp. the sensitivities of the in-house nested cppb gene and the 16s rrna pcr methods were greater than that of the amplicor n. gon ...19999889224
gram type-specific broad-range pcr amplification for rapid detection of 62 pathogenic bacteria.broad-range pcr has proven to be useful for the detection of bacteria. a set of broad-range pcr primers directed against conserved regions in the 16s rrna gene was designed to specifically amplify either gram-positive or gram-negative bacteria. the gram type-specific broad-range pcr correctly classified all 62 pathogenic species tested.19999889245
structural and evolutionary inference from molecular variation in neisseria porins.the porin proteins of the pathogenic neisseria species, neisseria gonorrhoeae and neisseria meningitidis, are important as serotyping antigens, putative vaccine components, and for their proposed role in the intracellular colonization of humans. a three-dimensional structural homology model for neisseria porins was generated from escherichia coli porin structures and n. meningitidis pora and porb sequences. the neisseria sequences were readily assembled into the 16-strand beta-barrel fold charac ...199910225902
purification and characterization of an esterase involved in cellulose acetate degradation by neisseria sicca sb.an esterase catalyzing the hydrolysis of acetyl ester moieties in cellulose acetate was purified 1,110-fold to electrophoretic homogeneity from the culture supernatant of neisseria sicca sb, which can assimilate cellulose acetate as the sole carbon and energy source. the purified enzyme was a monomeric protein with a molecular mass of 40 kda and the isoelectric point was 5.3. the ph and temperature optima of the enzyme were 8.0-8.5 and 45 degrees c. the enzyme catalyzed the hydrolysis of acetyl ...199910586499
carbonic anhydrase is an ancient enzyme widespread in prokaryotes.carbonic anhydrases catalyze the reversible hydration of co(2) and are ubiquitous in highly evolved eukaryotes. the recent identification of a third class of carbonic anhydrase (gamma class) in a methanoarchaeon and our present finding that the beta class also extends into thermophilic species from the archaea domain led us to initiate a systematic search for these enzymes in metabolically and phylogenetically diverse prokaryotes. here we show that carbonic anhydrase is widespread in the archaea ...199910611359
characterization of the ftsz cell division gene of neisseria gonorrhoeae: expression in escherichia coli and n. gonorrhoeae.we cloned the cell division gene ftsz of the gram-negative coccus neisseria gonorrhoeae (ng) strain ch811, characterized it genetically and phenotypically, and studied its localization in n. gonorrhoeae and escherichia coli (ec). the 1,179-bp orf of ftsz(ng) encodes a protein with a predicted molecular mass of 41.5 kda. protein sequence alignments indicate that ftsz(ng) is similar to other ftsz proteins and contains the conserved gtp binding motif. ftsz homologues were identified in several n. g ...200010648099
detection of legionella species in respiratory specimens using pcr with sequencing confirmation.legionella spp. are a common cause of community-acquired respiratory tract infections and an occasional cause of nosocomial pneumonia. a pcr method for the detection of legionellae in respiratory samples was evaluated and was compared to culture. the procedure can be performed in 6 to 8 h with a commercially available dna extraction kit (qiagen, valencia, calif.) and by pcr with gel detection. pcr is performed with primers previously determined to amplify a 386-bp product within the 16s rrna gen ...200010790085
gram-positive bacteria are potent inducers of monocytic interleukin-12 (il-12) while gram-negative bacteria preferentially stimulate il-10 production.interleukin-10 (il-10) and il-12 are two cytokines secreted by monocytes/macrophages in response to bacterial products which have largely opposite effects on the immune system. il-12 activates cytotoxicity and gamma interferon (ifn-gamma) secretion by t cells and nk cells, whereas il-10 inhibits these functions. in the present study, the capacities of gram-positive and gram-negative bacteria to induce il-10 and il-12 were compared. monocytes from blood donors were stimulated with uv-killed bacte ...200010816515
prokaryotic carbonic anhydrases.carbonic anhydrases catalyze the reversible hydration of co(2) [co(2)+h(2)oright harpoon over left harpoon hco(3)(-)+h(+)]. since the discovery of this zinc (zn) metalloenzyme in erythrocytes over 65 years ago, carbonic anhydrase has not only been found in virtually all mammalian tissues but is also abundant in plants and green unicellular algae. the enzyme is important to many eukaryotic physiological processes such as respiration, co(2) transport and photosynthesis. although ubiquitous in high ...200010978542
neisseria sicca meningitis in a woman with nascent pernicious anemia. 200011032571
a case of continuous ambulatory peritoneal dialysis peritonitis with an uncommon organism and an atypical clinical course.this report describes a 46-year-old patient who experienced an atypical course of peritonitis while undergoing continuous ambulatory peritoneal dialysis (capd). the first sign of peritonitis was progressive impairment of ultrafiltration with increasing fluid absorption. the patient came to the center after 5 days with leg edemas and 645 leukocytes/microl in the first dialysate outflow. on the same day, the dialysate cell count decreased to 208/microl. during the following days, ultrafiltration f ...200111136199
endocarditis due to rare and fastidious bacteria.the etiologic diagnosis of infective endocarditis is easily made in the presence of continuous bacteremia with gram-positive cocci. however, the blood culture may contain a bacterium rarely associated with endocarditis, such as lactobacillus spp., klebsiella spp., or nontoxigenic corynebacterium, salmonella, gemella, campylobacter, aeromonas, yersinia, nocardia, pasteurella, listeria, or erysipelothrix spp., that requires further investigation to establish the relationship with endocarditis, or ...200111148009
selective sensitization of bacteria to peroxide damage associated with fluoride inhibition of catalase and pseudocatalase.fluoride and sulfide are known inhibitors of heme catalases in acid environments. staphylococcus aureus h cells were found to be sensitized by fluoride or sulfide to h2o2 killing at acid ph values in the range of 3.5 to 4.0, and catalase activity was reduced concomitantly. in contrast, fluoride had little effect on h2o2 killing of streptococcus mutans gs-5, which has fluoride-insensitive peroxidase activity, but still is more sensitive to h2o2 than is s. aureus in the absence of fluoride. fluori ...200111169136
analysis of lipooligosaccharide biosynthesis in the neisseriaceae.neisserial lipooligosaccharide (los) contains three oligosaccharide chains, termed the alpha, beta, and gamma chains. we used southern hybridization experiments on dna isolated from various neisseria spp. to determine if strains considered to be nonpathogenic possessed dna sequences homologous with genes involved in the biosynthesis of these oligosaccharide chains. the presence or absence of specific genes was compared to the los profiles expressed by each strain, as characterized by their mobil ...200111208792
diagnostics of neisseriaceae and moraxellaceae by ribosomal dna sequencing: ribosomal differentiation of medical microorganisms.fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. however, in the case of some fastidious gram-negative bacterial species, classical phenotype identification based on either metabolic, enzymatic, or serological methods is difficult, time-consuming, and/or inadequate. 16s or 23s ribosomal dna (rdna) bacterial sequencing will most often result in accurate speciation of isolates. therefore, the objective of this study was to find a hypervariable ...200111230407
new tests for syphilis: rational design of a pcr method for detection of treponema pallidum in clinical specimens using unique regions of the dna polymerase i gene.a sensitive and specific pcr method to detect treponema pallidum in clinical specimens was developed. pcr primers were designed based on two unique features of the dna polymerase i gene (pola). the first distinctive characteristic is that the region codes for a high cysteine content and has low homology with similar regions of dna polymerase i gene from known microorganisms. the second unique feature is the presence of four insertions in the gene. pcr tests using primers designed on the basis th ...200111326018
enhancing the specificity of the cobas amplicor ct/ng test for neisseria gonorrhoeae by retesting specimens with equivocal results.the cobas amplicor ct/ng test for neisseria gonorrhoeae cross-reacts with certain strains of nonpathogenic neisseria species. in some strains, the target sequence is identical to that of n. gonorrhoeae, whereas other strains have a small number of mismatches within the regions recognized by the primers or probe used in the cobas amplicor ng test. these cross-reactive strains are occasionally present in urogenital specimens, causing false-positive results in the cobas amplicor ng test. analysis o ...200111526134
[utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients].the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ...200111594009
acid stress upregulated outer membrane proteins in clinical isolates of neisseria gonorrhoeae, but not most commensal neisseria.human immune serum recognition of outer membrane components from commensal and pathogenic neisseria cultured under neutral and acidic conditions was investigated. acid stress caused no detectable alterations in lipooligosaccharide migration and (or) staining, in outer membrane protein profiles, or in immune serum recognition of outer membrane components from neisseria mucosa or neisseria sicca. there was also no difference in the lipoologosaccharide electrophoretic pattern of acid- and neutral-g ...200111683469
comparative biology of incq and incq-like plasmids.plasmids belonging to escherichia coli incompatibility group q are relatively small (approximately 5 to 15 kb) and able to replicate in a remarkably broad range of bacterial hosts. these include gram-positive bacteria such as brevibacterium and mycobacterium and gram-negative bacteria such as agrobacterium, desulfovibrio, and cyanobacteria. these plasmids are mobilized by several self-transmissible plasmids into an even more diverse range of organisms including yeasts, plants, and animal cells. ...200111729261
detection of meningococcal carriage by culture and pcr of throat swabs and mouth gargles.the standard method for detecting meningococcal carriage is culture of throat swabs on selective media, but the levels of carriage determined depend heavily on the skills of the individuals taking the swab and interpreting the cultures. this study aimed to determine the most sensitive detection method for meningococcal carriage. throat swabs and saline mouth gargles, obtained from 89 university students, were processed in parallel by conventional culture and taqman ctra pcr. carriage of meningoc ...200211773095
purification and characterization of an endo-1,4-beta-glucanase from neisseria sicca sb that hydrolyzes beta-1,4 linkages in cellulose acetate.an enzyme catalyzing hydrolysis of beta-1,4 bonds in cellulose acetate was purified 18.3-fold to electrophoretic homogeneity from a culture supernatant of neisseria sicca sb, which can assimilate cellulose acetate as the sole carbon and energy source. the molecular mass of the enzyme was 41 kda and the isoelectric point was 4.8. the ph and temperature optima of the enzyme were 6.0-7.0 and 60 degrees c. the enzyme catalyzed hydrolysis of water-soluble cellulose acetate (degree of substitution, 0. ...200212005042
the class a macrophage scavenger receptor is a major pattern recognition receptor for neisseria meningitidis which is independent of lipopolysaccharide and not required for secretory responses.macrophages (mphi) play a key role in the pathogenesis of invasive meningococcal infections. the roles of two pattern recognition molecules, the mphi scavenger receptor (sr-a) and toll-like receptor 4 (tlr-4), have been investigated using bone marrow culture-derived mphi (bmmphi). surprisingly, a comparison of bmmphi from wild-type and sr-a knockout (sr-a(-/-)) mice showed that nonopsonic phagocytosis of meningococci was mediated almost exclusively via sr-a. previous studies have demonstrated on ...200212228258
assessment of the nasopharyngeal bacterial flora of rhesus macaques: moraxella, neisseria, haemophilus, and other genera.the nasopharyngeal bacterial flora of healthy rhesus macaques was surveyed for the presence of neisseria and haemophilus species, as well as moraxella catarrhalis. m. catarrhalis was found both in healthy rhesus macaques and in possibly immunocompromised rhesus macaques. several haemophilus spp. that are part of the normal nasopharyngeal bacterial flora of humans were found in many animals; these haemophilus species included h. parahaemolyticus, h. segnis, and h. parainfluenzae. while haemophilu ...200212409426
sulphonamide resistant commensal neisseria with alterations in the dihydropteroate synthase can be isolated from carriers not exposed to sulphonamides.development of sulphonamide resistance in neisseria meningitidis has been suggested to involve horizontal dna-transfer from a commensal neisseria species. in this study, we isolated commensal neisseria from throat specimens and examined the isolates with respect to sulphonamide resistance.200212435277
mosaic-like structure of penicillin-binding protein 2 gene (pena) in clinical isolates of neisseria gonorrhoeae with reduced susceptibility to cefixime.neisseria gonorrhoeae strains with reduced susceptibility to cefixime (mics, 0.25 to 0.5 micro g/ml) were isolated from male urethritis patients in tokyo, japan, in 2000 and 2001. the resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, n. gonorrhoeae atcc 19424, by transformation of the penicillin-binding protein 2 gene (pena) that had been amplified by pcr from a strain with reduced susceptibility to cefixime (mic, 0.5 micro g/ml). the sequences o ...200212435671
innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ...200212438343
identification of haemophilus influenzae serotypes by standard slide agglutination serotyping and pcr-based capsule typing.to resolve discrepancies in slide agglutination serotyping (sast) results from state health departments and the centers for disease control and prevention (cdc), we characterized 141 of 751 invasive haemophilus influenzae isolates that were identified in the united states from january 1998 to december 1999 through an active, laboratory-based, surveillance program coordinated by the cdc. we found discrepancies between the results of sast performed at state health departments and those of pcr caps ...200312517878
evaluation of the specificities of five dna amplification methods for the detection of neisseria gonorrhoeae.the intragenus specificities of five molecular diagnostic methods for neisseria gonorrhoeae were determined. three assays were considered suboptimal. molecular detection of n. gonorrhoeae from sites where other neisseria spp. commonly occur or from any site in low-prevalence settings should be confirmed by a test targeting a different genetic locus.200312574295
the moraxella catarrhalis immunoglobulin d-binding protein mid has conserved sequences and is regulated by a mechanism corresponding to phase variation.the prevalence of the moraxella catarrhalis immunoglobulin d (igd)-binding outer membrane protein mid and its gene was determined in 91 clinical isolates and in 7 culture collection strains. eighty-four percent of the clinical moraxella strains expressed mid-dependent igd binding. the mid gene was detected in all strains as revealed by homology of the signal peptide sequence and a conserved area in the 3' end of the gene. when mid proteins from five different strains were compared, an identity o ...200312644500
role of endo-1,4-beta-glucanases from neisseria sicca sb in synergistic degradation of cellulose acetate.an enzyme hydrolyzing beta-1,4 bonds in cellulose acetate was purified 10.5-fold to electrophoretic homogeneity from a culture supernatant of neisseria sicca sb, which assimilate cellulose acetate as the sole carbon and energy source. the enzyme was an endo-1,4-beta-glucanase, to judge from the substrate specificity and hydrolysis products of cellooligosaccharides, we named it endo-1,4-beta-glucanase i (eg i). its molecular mass was 50 kda, 9 kda larger than eg ii from this strain, and its isoel ...200312728982
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