Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| respiration and oxidative phosphorylation in treponema pallidum. | exogenous and endogenously generated reduced pyridine nucleotides caused marked stimulation of o(2) uptake when added to treponemal cell-free extracts, which indicated that terminal electron transport was coupled to the consumption of o(2). oxidation of reduced nicotinamide adenine dinucleotide (nadh) was shown to correlate stoichiometrically with o(2) reduction, suggesting that nadh was being oxidized through a mainstream respiratory chain dehydrogenase. oxygen evolution in treponemal extracts ... | 1978 | 29009 |
| pectinolytic enzymes of large rumen treponemes. | large spiral organisms isolated from the rumen of cattle produced and released into the external environment a complex of pectinolytic enzymes, consisting mainly of poly(1,4-alpha-d-galacturonide) lyase (ec 4.2.2.2, formerly ec 4.2.99.3), most active at ph 8.0 to 9.0, and another enzyme acting at ph below 7.0, probably a poly(1,4-alpha-d-galacturonide) glycanohydrolase (ec 3.2.1.15). the mixture of enzymes degraded polygalacturonate to saturated and unsaturated monogalacturonates as the end prod ... | 1979 | 32839 |
| the growth of treponema hyodysenteriae and other porcine intestinal spirochaetes in a liquid medium. | a new simple method for the preparation of a liquid medium containing rabbit serum for the propagation of treponema hyodysenteriae and other porcine intestinal spirochaetes is described. the medium, when dispensed in shallow layers and sealed under 10 per cent co2 in nitrogen, had a redox potential not greater than -125mv and an initial ph of about 6.9 when buffered with bicarbonate. growth of t hyodysenteriae developed more rapidly and viable counts reached higher levels at 42 degrees c than at ... | 1979 | 42126 |
| the ultrastructure of cultivable treponemes. | cells of treponema genitalis were studied in the electron microscope by means of negative staining and ultrathin sectioning techniques. all cells were covered by a regularly structured surface layer. this layer appeared to consist of pairs of thin fibrils attached to an amorphous layer. this amorphous layer in turn is probably identical with the exterior part of the outer membrane of the organism. the pairs of thin fibrils located on this surface were interconnected by polygons. the treponemes w ... | 1975 | 50715 |
| [studies of the ultrastructure of the treponema strain nichols]. | 1975 | 56124 | |
| fetal syphilis in the first trimester. | evidenc of first-trimester fetal syphilis was sought in the products of conception in a therapeutic abortion clinic. during two collection periods of one week, five patients with serologic and clinical data consistent with recent syphilitic infection were identified. their conceptuses were carefully examined by silver and immunofluorescent stains for the presence of treponema pallidum. two of these five conceptuses (9 and 10 weeks' gestation) were found to contain t. pallidum by these methods. t ... | 1976 | 56895 |
| determination by means of electron microscopy of morphological criteria of value for classification of some spirochetes, in particular treponemes. | 1976 | 58539 | |
| new source for positive controls used for the identification of spirochaetes in tissue sections. | lyophilized t. pallidum is recontituted or purchased as a suspension, evenly dispersed, made into smears using alcohol-cleaned slides and a bacteriological platinum wire loop. smears are air dried for 15 minutes and fixed in concentrated formaldehyde fumes for 30 minutes and stored at room temperature. these preparations are used as positive controls for conventional silver impregnation techniques recommended for spirochaete detection in tissue sections. | 1976 | 59412 |
| [current serology of syphilis. tests, specific verification tests, therapy-leading reactions]. | 1976 | 62696 | |
| the jarisch-herxheimer reaction. | 1977 | 64863 | |
| immunofluorescent staining of treponema pallidum and treponema pertenue in tissues fixed by formalin and embedded in paraffin wax. | the main problems in identifying treponema pallidum in tissues are optical definition contrast, and specificity. in general, fluorochrome staining provides optical definition and contrast superior to that obtained by ordinary tinctorial staining, and in theory improved resolution. specificity is lacking however, as with other stains. in contrast, immunofluorescence should combine the optical advantages of fluorochrome staining with the immunological advantages of specificity. since the validity ... | 1977 | 66082 |
| [staining effects of various dyes on treponema pallidum (a pathogenic nichols strain)]. | 1978 | 80462 | |
| neurosyphilis today. | 17 cases of recently diagnosed neurosyphilis are reviewed. the presenting features are diverse, and a clinical diagnosis may be difficult. routine serological test are essential to confirm the clinical diagnosis. | 1979 | 84139 |
| immunoperoxidase localization of treponema pallidum: its use in formaldehyde-fixed and paraffin-embedded tissue sections. | the spirochete, treponema pallidum, the causative agent of syphilis, has been successfully localized in formaldehydefixed and paraffin-embedded tissue sections using rabbit anti-t pallidum antiserum with two immunoperoxidase techniques. these techniques, the indirect peroxidase-labeled antibody method and the peroxidase-antiperoxidase (pap) method are compared for sensitivity and degree of nonspecific staining. both offer substantial advantages over conventional silver-impregnation techniques, b ... | 1979 | 85440 |
| [antigenic properties of a new strain (central skin and venereological research institute-74) of pathogenic treponema pallidum in specific reactions for syphilis]. | 1979 | 90436 | |
| surface-associated host proteins on virulent treponema pallidum. | a surface coat of host serum proteins was detected on virulent treponema pallidum by sodium dodecyl sulfate-gel electrophoresis. the loosely associated serum proteins could be removed by repeated washings in a protein-free medium. washed t. pallidum retained the ability to readsorb numerous host proteins from rabbit serum as well as iodinated rabbit or human albumin. in addition, various avidly associated host serum proteins including albumin, alpha(2)-macroglobulin, transferrin, ceruloplasmin, ... | 1979 | 93574 |
| ability of enriched immune t cells to confer resistance in hamsters to infection with treponema pertenue. | this investigation presents the first direct evidence that t cells are involved in resistance to challenge with treponema pertenue. enriched t cells from immune hamsters were obtained by sequential filtration through glass and nylon-wool columns. this procedure removed the majority of functional antibody-producing and immunoglobulin-bearing cells. the fractionated cell suspensions were less responsive to stimulation by phytohemagglutinin, lipopolysaccharide, and dextran sulfate, but they were en ... | 1979 | 94906 |
| comparison of antigenic properties among various strains of treponema hyodysenteriae. | agglutinin-absorption and precipitin-absorption studies demonstrated that three strains of treponema hyodysenteriae isolated from cases of swine dysentery were antigenically different from each other and also from a spirochete isolated from a clinically normal dog. each of the strains of t. hyodysenteriae also possessed two common antigens. | 1979 | 95077 |
| [personal prophylaxis of venereal diseases]. | 1978 | 97875 | |
| morphology of freeze-etched treponema refringens (nichols). | the freeze-etch technique was used to study the morphology of treponema refringens (nichols). there is a single band of cytoplasmic fibrils which follows a path in the form of a right-handed helix with a periodicity of 1500 nm around the body of the treponeme just below the cytoplasmic membrane. there are two major fracture planes, one located in the interior of the outer envelope and the second in the interior of the cytoplasmic membrane. the "blebs" or "surface protuberances", which are quite ... | 1978 | 100069 |
| [etiology of porcine dysentery]. | the etiological agent of swine dysentery is an anaerobic spiralshaped microorganism of the treponema genus. examination of a scraping from the mucous membrane of the large intestine or the examination of faeces in a native preparation by phase contrast are the fastest diagnostical methods of determining swine dysentery. large treponemas, as predominating microflora, 6--9 micrometer long, 0.3--0.4 micrometer in diameter, spiralshaped and moving in a serpentine way are found in faeces and the muco ... | 1979 | 105447 |
| [isolation of treponemas from the colon of pigs with clinical dysentery]. | optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ... | 1979 | 105448 |
| [comparison of the suitability of phase-contrast and immunofluorescence technics in the laboratory diagnosis of porcine dysentery]. | immunofluorescence technique, compared with the method of phase contrast, does not appear to be better for laboratory diagnostics of swine dysentery because neither of these methods can be used for distinguishing between pathogenic and nonpathogenic strains of treponemas. the number of treponemas contained in faeces should still be considered to be the main criterion in laboratory diagnostics. in clinically healthy pigs from stocks which never suffered from dysentery treponemas were found only i ... | 1979 | 105449 |
| [the current situation of syphilis. diagnosis by exact serological studies or direct demonstration of treponemas]. | 1979 | 114807 | |
| serotypes of beta-hemolytic treponema hyodysenteriae. | cultures form 13 isolates of pathogenic, beta-hemolytic treponema hyodysenteriae from 11 geographically separate outbreaks and 2 experimentally induced cases of swine dysentery were lyophilized and extracted with hot phenol-water. the resulting water phases were examined serologically with antisera produced in rabbits against whole-cell bacterins of the 13 isolates for evidence of antigenic classes within the species. water-phase antigens gave precipitin reactions with homologous antisera. resul ... | 1979 | 115788 |
| partial purification of a specific antigen of treponema hyodysenteriae. | a specific antigen of pathogenic treponema hyodysenteriae was extracted from lyophilized cells of isolant b169 with hot phenol-water, isolated, and semipurified by starch block electrophoresis. | 1979 | 118931 |
| experimental syphilis in the rabbit: passive transfer of immunity with immunoglobulin g from immune serum. | a preparation of immunoglobulin g isolated from a pool of immune sera derived from rabbits with long-term syphilis was shown to possess a high degree of purity as judged by immunodiffusion and protein electrophoresis. the antitreponemal power of the preparation of immunoglobulin g and that of the immune serum pool from which it was derived were found to be equivalent in both the skin protection and the systemic protection test. the observation that neither normal serum nor a pool of serum derive ... | 1979 | 120386 |
| treponema succinifaciens sp. nov., an anaerobic spirochete from the swine intestine. | the morphology, the general physiological characteristics, and the energy-yielding metabolism of an obligately anaerobic spirochete isolated from the colon of a swine were studied. electron microscopy showed that the helical spirochetal cells possessed an outer sheath, a protoplasmic cylinder, and 4 periplasmic fibrils in a 2-4-2 arrangement. the spirochete grew in an atmosphere of n2 in prereduced media containing a carbohydrate, nahco3, rumen fluid, yeast extract, peptone, l-cysteine, and inor ... | 1979 | 120726 |
| [electroimmunodiffusion with reiter treponema antigen shows serum immunoglobulin isolated from subjects with secondary syphilis]. | the results of electroimmunodiffusion investigations with reiter treponema antigen and syphilitic subject sera are presented. counter-immunoelectrophoresis, rocked electrophoresis and crossed-electrophoresis studies have been performed. the test positivity is shown by the appearance of one ore two precipitates. no aspecificities were remarked with the latter two techniques: particular attention was devoted to the preparation of the treponema antigen in the attempt to eliminate the aspecificity w ... | 1979 | 121912 |
| effect of spectinomycin on t. pallidum in incubating experimental syphilis. | animal experiments were performed to determine if a single-dose treatment for acute gonorrhoea with 2 g. spectinomycin could cure a simultaneously acquired syphilis at a very early stage of incubation. 300 treponemes (nichols stain t. pallidum) were inoculated intratesticularly and 3 days later spectinomycin was administered in a dose which produced spectinomycin serum levels similar to those in patients who had received a single oral dose of 2 g. this dosage of spectinomycin did not prevent the ... | 1975 | 127646 |
| effect of lincomycin and spectinomycin on swine dysentery. | 1975 | 127771 | |
| treponemal antigen in immunopathogenesis of syphilitic glomerulonephritis. | a patient with syphilitic glomerulonephritis had a renal biopsy and was treated for secondary syphilis. light, electron, and immunofluorescence microscopic studies revealed an acute proliferative glomerulonephritis with subepithelial, intramembranous, and subendothelial immune complex deposits containing igg, iga, igm, c4, and c3. similar local deposits containing predominantly igm were noted in areas of mesangial proliferation. indirect fluorescent antibody studies employing rabbit treponemal a ... | 1976 | 130804 |
| scanning electron microscopy of the lesions of swine dysentery. | thirty weanling pigs were examined by scanning electron microscopy at various time intervals after oral inoculation with crude colon contents from pigs affected with dysentery. the earliest recognizable change was a corrugated appearance of the mucosal surface of the large intestine. large spirochetes, morphologically similar to treponema hyodysenteriae, were first observed within the crypts of lieberkühn where they seemed to proliferate onto the luminal surface. then mucus, fibrin, erythrocyte ... | 1976 | 131500 |
| drugs five years later. spectinomycin. | a single intramuscular injection of 0.2 g of spectinomycin hydrochloride is highly effective for the treatment of uncomplicated anogenital infections with neisseria gonorrhoeae. spectinomycin hydrochloride is indicated for uncomplicated anogenital gonococcal infections in men and women who cannot receive penicillin or probenecid, and is the drug of choice for the retreatment of patients with uncomplicated anogenital gonococcal infection who have not responded to other antibiotics. a single dose ... | 1976 | 132888 |
| selective medium for isolation of treponema hyodysenteriae. | pure cultures of six pathogenic isolates of treponema hyodysenteriae, the colonic mucosal scrapings of seven pigs with acute swine dysentery, and feces from seven unaffected pigs were diluted in phosphate-buffered saline and plated on trypticase soy agar with 5% citrated bovine blood (tsa) and tsa with various levels of spectinomycin (tsa-s). the plates were incubated at 42 degrees c in a vented gaspak jar with a cold palladium catalyst and either 80:20 h2-co2 by evacuation and refilling or a h2 ... | 1976 | 134043 |
| [application of the immunofluorescente (fluorescent antibody technique) in clinical immunology.--ii. application and significance (author's transl)]. | the fluorescent antibody technique is nowadays mainly a technique, which is routinely carried out in all clinical-immunological laboratories. the main fields of application in clinical immunology are the determination of autoantibodies (e.g. ana, ama, smooth muscle antibodies, parietal cell antibodies), detection of b-lymphocytes as well as bacterial antigens (e.g. treponema pallidum). furthermore the demonstration of deposits of immune complexes in tissues (e.g. glomerulonephritis, periarteriit ... | 1976 | 134971 |
| inability of spleen cells from chancre-immune rabbits to confer immunity to challenge with treponema pallidum. | although several lines of evidence suggest that cellular immune mechanisms play a role in controlling infection due to treponema pallidum, recent studies have shown that induction of acquired cellular resistance by antigenically unrelated organisms fails to protect rabbits against syphilitic infection, thereby casting doubt on this hypothesis. in the present paper we describe attempts to transfer immunity to syphilis by using spleen cells from chancre-immune rabbits. intravenous infusion of 2 x ... | 1977 | 143456 |
| [serological diagnosis of syphilis (author's transl)]. | 1977 | 144876 | |
| parenteral immunization of pigs against infection with treponema hyodysenteriae. | six intravenous injections of formalin-inactivated treponema hyodysenteriae were given to 8 specific-pathogen-free pigs at 6-day intervals. the 8 vaccinated and 8 control pigs were challenged intragastrically with pure cultures of t hyodysenteriae 7 and 8 days after the last intravenous injection. clinical signs of swine dysentery were observed in all 8 control pigs, but was observed in only 1 of the immunized pigs. three control pigs died. these findings suggest that parenteral immunization wit ... | 1978 | 148230 |
| [the test combination of cardiolipin microflocculation, tpha and fta-abs in the diagnosis of syphilis--report on one year's experience (author's transl)]. | 1978 | 149272 | |
| fibrinolytic activity of oral anaerobic bacteria. | 1978 | 152102 | |
| mucopolysaccharide material resulting from the interaction of treponema pallidum (nichols strain) with cultured mammalian cells. | during incubation of treponema pallidum (nichols strain) with cultured mammlian cells derived from normal rabbit testes (nrt), an amorphous material accumulated at the surface of the cultured cells. this material was randomly distributed on all tissue cells within the culture chambers. the amount of amorphous material was dependent on the treponemal inocula. with 3 x 10(8) organisms per ml, this material was readily apparent within 2 days; with 4 x 10(7) organisms per ml, this material was detec ... | 1978 | 153334 |
| surface mucopolysaccharides of treponema pallidum. | the viscous mucoid fluid that accumulates within syphilitic lesions may be due to breakdown of host tissue during infection, or may be synthesized by treponema pallidum. experiments were performed to investigate the acidic mucopolysaccharides that occur at the surface of t. pallidum (nichols strain). these mucopolysaccharides were demonstrated by reaction with acidified bovine serum albumin and by agglutination with wheat germ agglutinin and soybean agglutinin. the polycations ruthenium red and ... | 1979 | 156696 |
| relationship of treponema pallidum to acidic mucopolysaccharides. | attempts were made to relate treponema pallidum to the acidic mucopolysaccharides that occur in vivo within host ground substance and in vitro on the surface of cultured testicular cells. infected testicular tissue was fixed and processed for transmission electron microscopy in the presence of ruthenium red. the use of this inorganic dye demonstrated the large quantity of mucopolysaccharide within testicular tissue and the intimate association of treponemes with this material. wheat germ aggluti ... | 1979 | 156697 |
| mucopolysaccharidase of treponema pallidum. | treponema pallidum (nichols strain) exhibited mucopolysaccharidase activity. acidic mucopolysaccharides were broken down more rapidly by viable treponemes than by heat-inactivated treponemes or membrane filtrates of treponemal suspensions. ouchterlony immunodiffusion demonstrated the occurrence of antibodies to the hyaluronidase-like enzyme within syphilitic sera. after intratesticular inoculation of 2 x 10(7) to 6 x 10(7) treponemes, these anti-mucopolysaccharidase antibodies were detected betw ... | 1979 | 156698 |
| aberrant secondary antibody responses to sheep erythrocytes in rabbits with experimental syphilis. | rabbits infected with treponema pallidum have strikingly depressed in vivo immunoglobulin g responses to sheep erythrocytes. to gain further insight into the nature of this suppression, the immune responses of splenic and peripheral blood lymphocytes from infected rabbits to sheep erythrocytes were studied in vitro. spleen cells from rabbits that had been sensitized with sheep erythrocytes during active syphilis had greatly decreased immunoglobulin m and g responses after in vitro incubation wit ... | 1979 | 157977 |
| suppression of lymphocyte response to concanavalin a by mucopolysaccharide material from treponema pallidum-infected rabbits. | the testicular fluid and serum from rabbits infected intratesticularly with treponema pallidum inhibited the mitogenic response of normal rabbit peripheral blood lymphocytes to concanavalin a. mucopolysaccharide material present in the testicular fluid and serum was associated with the lymphocyte-inhibitory activity. degradation of the mucopolysaccharide material with hyaluronidase resulted in the loss of the inhibitory activity of testicular fluid and serum of t. pallidu-infected rabbits. | 1979 | 159264 |
| [screening examinations for identifying syphilis antibodies in newborn blood dried on filter paper (author's transl)]. | 1979 | 161383 | |
| infectious diseases in primitive societies. | incidence of various infectious diseases in several amazon indian tribes has been determined serologically. diseases that infect only man fall into two distinct categories. those which can persist in an individual for a prolonged period are highly endemic, but those which are infectious only in the acute phase die out quickly after introduction. the suggestion is made that the latter diseases could not perpetuate themselves before the advent of advanced cultures and did not exert selective press ... | 1975 | 163483 |
| letter: anergy in patients with syphilis. | 1975 | 165417 | |
| diagnosis of chronic perinatal infections. | 1975 | 165713 | |
| intracellular treponema pallidum in cells of a syphilitic lesion of the uterine cervix. | in an electron microscopic study of biopsy tissue from an ulcer of the human cervix uteri, organisms morphologically identical to t. pallidum were observed. large numbers of the organisms were seen lying among collagen fibrils and between cells. t. pallidum was seen within vacuoles of some fibroblasts and epithelial cells. within the nucleoplasm of several cells, treponemes were observed, surrounded by a clear zone which might represent a mucopolysaccharide outer layer. the siameter of the organ ... | 1975 | 165724 |
| investigations on in vitro survival and virulence of t. pallidum under aerobiosis. | motility of pathogenic t. pallidum was maintained in aerobic in vitro cultures for several weeks using a special medium. the latter consisted of mccoy's 5a medium supplemented with glutathione, sodium pyruvate, hepes buffer, gentamycin (garamycin), and fetal calf serum. the virulence of the organisms was lost in 5 to 6 days. no multiplication of the organisms was observed. four antibiotics (viomycin, kanamycin, gentamycin (garamycin), and neomycin) were tested for their bactericidal action and p ... | 1975 | 172190 |
| effect of pretreatment with mycobacterium bovis (strain bcg) and immune syphilitic serum on rabbit resistance to treponema pallidum. | stimulation of the rabbit reticuloendothelial system with viable mycobacterium bovis (strain bcg), and other agents, had no effect on the development of syphilitic lesions after intradermal or intravenous inoculation with graded doses of treponema pallidum (virulent nichol's strain; mean infective doses less than 10). the simultaneous administration of immune syphilitic rabbit serum retarded the development of lesions, but this appeared to be due solely to the immune serum, suggesting no synergi ... | 1975 | 172450 |
| [seroepidemiological studies related to the association of genital herpes to cervical cancer (author's transl)]. | blood sera of 290 patients were examined for the presence of antibodies against herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2): 1. lues - seronegative individuals, 2. patients with specific lues antibodies, and 3. patients with so-called carcinoma in situ and invasive carcinoma of the uterine cervix. the group of syphilis-seropositive individuals served as control for the group with cervical lesions, because in both groups certain socioepidemiologic factors (promiscuity, early sexual i ... | 1975 | 174969 |
| sero-immunological investigations in patients with cervical cancer: higher rate of hsv-2 antibodies than in syphilis patients and evidence of igm antibodies to an early hsv-1 antigen. | 1975 | 191383 | |
| the fta-abs test: a diagnostic help or hindrance? | the fluorescent treponemal antibody absorption (fta-abs) test, an excellent confirmatory treponemal test, has been used increasingly for syphilis screening and case detection. to evaluate its performance as an initial test, we did venereal disease research laboratory (vdrl) slide and fta-abs tests on 1,043 patients suspected of having syphilis. when retested in both a local and a reference laboratory, sera from 226 patients with borderline or reactive results demonstrated interlaboratory consist ... | 1977 | 191932 |
| terminal electron transport in treponema pallidum. | reduced-minus-oxidized difference spectra of sonically treated virulent treponema pallidum disclosed cytochromes of the b anc c types as well as large amounts of flavoprotein. difference spectra of the carbon monoxide-binding pigment identified cytochrome o as the terminal oxidase. physiological reduction of the cytochromes indicated that the cytochrome system was functional and established the capability of t. pallidum for aerobic respiration. the potential significance of these findings is dis ... | 1977 | 197010 |
| the prevalence of herpesvirus hominis in genital lesions with suggestive chancre morphology. | 1977 | 200136 | |
| the microbiological flora of penile ulcerations. | the penile ulcerations of 100 consecutive men were tested for microorganisms. a polymicrobial flora was identified in the ulcers of 97 men. the microorganisms recovered from these ulcers included combinations of anaerobic and aerobic bacteria (including mycoplasma), herpes simplex virus, yeasts, and filamentous fungi. fifty-three study entrants had microorganisms, identified by culture or serologic tests, that were considered primary in ulcer pathogenesis. herpes simplex virus was the most preva ... | 1978 | 203634 |
| a new technique of heterogenous enzyme-linked immunosorbent assay, stick-elisa. ii. application of stick-elisa to antigens of various infective agents. | stick-enzyme-linked immunosorbent assay (stick-elisa) was applied to antigens of a variety of infective agents. an acceptable reproducibility in a series was reached, the variation coefficient being twelve percent. preliminary results revealed that ready coated sticks could be stored at 4 degrees c for at least two to three weeks. | 1978 | 205062 |
| serum of rabbits infected with treponema pallidum (nichols) inhibits in vitro transformation of normal rabbit lymphocytes. | 1979 | 218739 | |
| simultaneous infection with treponema pallidum and herpes simplex virus. | two cases are presented of simultaneous infection with treponema pallidum and herpes simplex virus. the occasional concurrence of these diseases suggests that all cases of herpes genitalis, including those with negative darkfield examinations and initial nonreactive serologic tests for syphilis, should be followed in two to four weeks by a repeat serologic test for syphilis. | 1979 | 225130 |
| effect of kcn on the motility of treponema pallidium. | virulent treponema pallidum has been shown to survive in kcn-containing artificial medium. oxygen uptake being sensitive to cyanide, the observation indicates that treponemes do not have a cytochrome oxidase system. kcn had a killing effect only at a 15--30 mm final concentration. the data show that the budapest strain of t. pallidum is an anaerobic organism. | 1979 | 232617 |
| preference for basic igg in early syphilis. | by differential igg measurements of sera of 27 patients with early infectious syphilis, it was found that infection with t. pallidium results in a preferential synthesis of igg immunoglobulins characterized by high isoelectric points. the decrease in total igg level observed after 6 weeks of treatment can to a large extent be accounted for by the decrease in basic igg concentration. the relationship between the total and basic igg levels and the number of t. pallidum present in the body during t ... | 1975 | 237600 |
| hypergammaglobulinemic renal tubular acidosis. association with takayasu arteritis. | 1975 | 241867 | |
| staining large spirochetes in fecal and colonic scrapings with victoria blue 4-r: an aid in the diagnosis of swine dysentery. | 1978 | 245891 | |
| in vivo and in vitro susceptibility of treponema hyodysenteriae to carbadox before and after repeated in vitro passage in sublethal concentrations of drug. | 1978 | 249133 | |
| use of oral vaccines in attempts to prevent swine dysentery. | 1979 | 260870 | |
| [anaerobic infections of month and pharynx]. | 1978 | 284632 | |
| selective response of lymphocytes from treponema pallidum-infected rabbits to mitogens and treponema reiteri. | the in vitro response of peripheral blood lymphocytes from rabbit infected with treponema pallidum was examined using various mitogens and avirulent treponema reiteri. for the first 4 weeks after treponemal infection, the response of lymphocytes from syphilitic rabbits to phytohemagglutinin and pokeweed mitogen was markedly reduced in comparison to uninfected controls. lymphocytes from both groups of rabbits responded normally to class-specific immunoglobulin anti-sera (anti-immunoglobulin m and ... | 1977 | 300359 |
| in vitro cell response of treponema pallidum-infected rabbits. iii. impairment in production of lymphocyte mitogenic factor. | production of mitogenic factor was examined in rabbits infected intratesticularly with t. pallidum and in control animals injected with saline or saline extract of normal rabbits' testes. lymph nodes and spleen from animals killed 2, 6 and 12 weeks after injection were used as the source of lymphocytes, cultured in serum-free medium in the presence of reiter antigen. the active supernatants of lymph node cells (lnas) and spleen cells (spas) were examined for the presence of mitogenic factor usin ... | 1977 | 303968 |
| [sero-epidemiological study on the endemic occurrence of treponema infections in senegal]. | 1979 | 312164 | |
| inability of immune cells treated with anti-thymocyte serum to confer on hamsters resistance to cutaneous infection with treponema pertenue. | the mechanism by which hamsters acquire resistance to yaws or frambesia is poorly understood. this investigation has shown that immune lymphoid cells (spleen and lymph node) could confer on hamsters resistance to infection with treponema pertenue. treatment of these immune cells with a specific antithymocyte serum (ats) inhibited the transfer of resistance. twenty-one days after infection, recipients of immune cells treated with ats had cutaneous lesions, in contrast to recipients of immune cell ... | 1979 | 314420 |
| interaction of treponema pallidum (nichols strain) with cultured mammalian cells: effects of oxygen, reducing agents, serum supplements, and different cell types. | cultured mammalian cells extend the time of survival of treponema pallidum (nichols strain). various parameters that have been previously shown to enhance treponemal survival in vitro were examined for influences on the interaction of t. pallidum with cultured cells. with cells derived from normal rabbit testes, the time of retention of treponemal virulence was extended in an atmosphere containing reduced concentrations of oxygen. glutathione and cysteine, when added to the basal tissue culture ... | 1977 | 321350 |
| immune lysis of spin label loaded liposomes incorporating cardiolipin; a new sensitive method for detecting anticardiolipin antibodies in syphilis serology. | liposomes prepared from a mixture of the pure lipids cholesterol, lecithin and cardiolipin (molar ratio 50/45/5), are able to bind antibodies directed against treponema pallidum. when the liposomes are loaded with the water soluble spin label tempocholine chloride, the release of spin label from the liposomes can be monitored directly by observing changes in the paramagnetic resonance (esr) spectrum from the spin label. the method offers a convenient technique for monitoring the complement-media ... | 1977 | 321698 |
| [evaluation of inter-laboratory repeatability of fta-abs and fta tests in polish serological laboratories in 1974-1975]. | 1977 | 322220 | |
| [present-day views on the sensitivity and specificity of treponemal tests and the range of their use in diagnostics]. | 1977 | 322221 | |
| spinal syphilis: the problem of fluorescent treponemal antibody in the cerebrospinal fluid. | spinal manifestations of syphilis are now uncommon. three adults with presumptive nontabetic spinal syphilis are presented. this paper should serve as a reminder to physicians that cases of late syphilis continue to occur and may be manifested as obscure spinal syndromes and be misdiagnosed unless the possibility of syphilis is constantly kept in mind. some of the clinical dilemmas associated with the reactivity of fluorescent treponemal antibody in the cerebrospinal fluid are discussed, | 1977 | 322296 |
| the effect of treponema pallidum on mouse survival. | intraperitoneal infection with treponema pallidum did not shorten the lives of inbred cba mice. one hundred and fifty infected mice survived to a mean age of 41-24 months and 60 uninfected mice to a mean age of 41-38 months. | 1977 | 323491 |
| serologic tests in late syphilis. | 1977 | 324457 | |
| physiology and evolution of spirochetes. | 1977 | 324466 | |
| scanning electron microscopy of treponema pallidum (nichols strain) attached to cultured mammalian cells. | this paper describes the attachment of treponema pallidum (nichols strain) to cultured mammalian cells as a visualized by scanning electron microscopy. treponemes were incubated for 3 hr with cultured cells derived from normal rabbit testes or human skin epithelium, then fixed, processed with critical-point drying, and examined with a cambridge mark 2a scanning electron microscope. large numbers of treponemes became attached to the cultured cells without altering the morphological integrity of t ... | 1977 | 324985 |
| re: persistence of treponema pallidum following penicillin g therapy. | 1977 | 325237 | |
| cerebrospinal fluid serology--is its routine use justifiable? | although neurospyhilis has become a rare disease, requests for cerebrospinal fluid (csf) syphilis serology tests at the colorado department of health have increased in recent years. because of the low rate of positive results, questionnaires were sent to the physicians who requested these tests to determine the criteria for their use. neurosyphilis was neither suspected nor an important consideration to rule out in 63 percent of the 337 patients sampled. of the patients whose ages were specified ... | 1977 | 325590 |
| [treponemal hemagglutination test (tpha). validity of the micromethod using dilutions of sera 1/20-1/40]. | the authors studied the possibility to use, for the carrying out of the tpha micromethod, higher serum dilutions, as preliminary investigations had pointed out an insufficient sensitivity of the reaction performed at the 1:80 - 1:160 dilutions, particularly in the primary-secondary phase of the luetic infection. the authors controlled, preliminarily, that at the dilutions studied, no unacceptable increase of spontaneous hemagglutinations and false positive reactions took place in the test sera. ... | 1977 | 326267 |
| catabolism of glucose and fatty acids by virulent treponema pallidum. | we describe a procedure which permits essentially full recovery of physiologically active treponema pallidum from rabbit testicular extracts and greatly reduces contaminating tissue material. such preparations were employed for investigations of the ability of t. pallidum to catabolize glucose and fatty acids. radiorespirometric studies revealed that glucose and pyruvate, but not oleate or palmitate, could be degraded to co2. the use of differentially labeled glucose, in conjunction with enzymat ... | 1977 | 326678 |
| [serodiagnosis of syphilis from the viewpoint of the immunologist]. | a review of the immunology of the serological diagnosis of syphilis is given and modern methods for the demonstration of treponema-specific antibodies are shown. the possible combinations of test results and interpretations of these are discussed. the importance of the demonstration of treponema-specific igm-antibodies not only for diagnosis but also for basis of treatment is finally pointed out. | 1977 | 326726 |
| specific direct fluorescent antibody detection of treponema pallidum. | in a study to determine the reliability of specific direct fluorescent antibody staining of treponema pallidum (dfatp) in lesion exudate, and to evaluate it as a potential diagnostic test to be used in lieu of or as an adjunct to the darkfield and other laboratory tests as an aid in the diagnosis of early syphilis, two types of comparisons were made: study a-replicate specimens from each of 350 lesions were examined in the state laboratory by dfatp and in the field by darkfield microscopy and th ... | 1977 | 326728 |
| serologic study of specimens with borderline fta-abs test reactivity. | the treponemal group-specific absorbed fluorescent antibody test (fta-abs) does not yield clearly positive or negative results in all instances. this study was designed to analyze those sera showing borderline reactivity, in order to determine whether additional tests may help to resolve serodiagnostic problem cases. fta-abs tests on 23,807 sera submitted to the wisconsin state laboratory of hygiene yielded borderline results with 479 specimens (2%). patients showing inconclusive fta-abs test re ... | 1977 | 326729 |
| [serologic diagnosis of syphilis; method and interpretation]. | 1977 | 327341 | |
| cell-mediated immunity in experimental syphilis in rabbits. | the development of cell-mediated immunity was studied in rabbits: a) experimentally injected with t. pallidum, and b) artificially immunized with nonviable t. pallidum. the macrophage migration inhibition test (mmi test) using ultrasonicate of t. pallidum as antigen was employed to demonstrate this type of immunologic response. lymphocytes of syphilitic rabbits were found to exert a pronounced inhibitory effect upon macrophage migration as early as one month after infection; between the fourth a ... | 1977 | 327968 |
| [technical advances in demonstration of treponema-specific 19s-igm antibodies in patients with latent or late-latent syphilis (author's transl)]. | in patients with latent or late-latent syphilis as well as neurosyphilis the demonstration of treponema-specific 19s-igm antibodies is not regularly possible. in this publication it is shown that high igg antibodies are the reason for a competitive igm inhibition in the indirect immunofluorescence tests. it is demonstrated that in the above mentioned states of the infection the igm-fta-19s test seemed to be the method of choice to realize the existence of 19s-igm antibodies. in sera with competi ... | 1977 | 328375 |
| parasitism by virulent treponema pallidum of host cell surfaces. | the interaction between virulent treponema pallidum extracted from infected rabbit testes and animal cells in culture was examined. the extent of treponemal attachment to monolayers of normal rabbit testicular and hep-2 cells was dependent upon the incubation temperature and retained motility of the spirochetes. the specific orientation of treponemes to host cell surfaces was demonstrated by dark-field microscopic examination of wet-mount preparations and scanning and transmission electron micro ... | 1977 | 328394 |
| rapid detection of specific treponemal antibodies by counterimmunoelectrophoresis using an extract of treponema pallidum. | specific treponemal antibodies were detected in small amounts by counterimmunoelectrophoresis (cie) in agar gel, using an extract of treponema pallidum. the t. pallidum-cie test was compared with the fluorescent treponemal antibody-absorption (fta-abs) test and with the reiter protein complement fixation (rpcf) test. the results of the t. pallidum-cie and fta-abs tests agreed in 96% of cases. there was no agreement between the t. pallidum-cie and rpcf tests in 14% of cases. the three tests were ... | 1977 | 328449 |
| comparison between the automated reagin test and reagin screen test methods of vdrl screening tests for syphilis in use in a routine laboratory. | a comparison is made between the automated reagin test (art) using technicon autoanalyzer equipment and the reagin screen test (rst) introduced by lederle using a new antigen formulation. treponemal haemagglutination tests (tpha) were done simultaneously as second screen tests. the absorbed fluorescent treponemal antibody test (fta-abs) was used in all seropositive cases. altogether 1154 sera were tested; 1028 were negative with all tests, 66 were positive with all tests, 42 were positive with t ... | 1977 | 328538 |
| [normal mouth flora and its changes in gingivitis]. | 1977 | 329365 | |
| luetic labyrinthitis. | 1977 | 329459 | |
| [fluoresence microscopic studies on the proof of syphilis-specific antibodies by means of fitc-labeled igg, igm and and iga immune serums with regard to the current level of immunoglobulins]. | 1977 | 329596 | |
| summary of the workshop on the biology of treponema pallidum: cultivation and vaccine development. | 1977 | 330771 |