Publications

novel reassortant influenza a(h5n8) viruses in domestic ducks, eastern china.	domestic ducks are natural reservoirs of avian influenza viruses and serve as reassortant hosts for new virus subtypes. we isolated 2 novel influenza a(h5n8) viruses from domestic ducks in eastern china, sequenced their genomes, and tested their pathogenicity in chickens and mice. circulation of these viruses may pose health risks for humans.	2014	25075453
characterization of an h5n8 influenza a virus isolated from chickens during an outbreak of severe avian influenza in japan in april 2014.	a highly pathogenic avian influenza virus (hpaiv) of subtype h5n8, a/chicken/kumamoto/1-7/2014, was isolated from a japanese chicken farm during an outbreak in april 2014. phylogenetic analysis revealed that this virus belonged to ha clade 2.3.4.4. all eight genomic segments showed high sequence similarity to those of the h5n8 subtype hpaivs a/broiler duck/korea/buan2/2014 and a/baikal teal/korea/donglim3/2014, which were isolated in korea in january 2014. intranasal experimental infection of ch ...	2015	25902725
pathogenicity of h5n8 highly pathogenic avian influenza viruses isolated from a wild bird fecal specimen and a chicken in japan in 2014.	poultry outbreaks caused by h5n8 highly pathogenic avian influenza viruses (hpaivs) occurred in japan between december 2014 and january 2015. during the same period; h5n8 hpaivs were isolated from wild birds and the environment in japan. the hemagglutinin (ha) genes of these isolates were found to belong to clade 2.3.4.4 and three sub-groups were distinguishable within this clade. all of the japanese isolates from poultry outbreaks belonged to the same sub-group; whereas wild bird isolates belon ...	2016	26916882
transmission of an h5n8-subtype highly pathogenic avian influenza virus from infected hens to laid eggs.	we showed here that an h5n8-subtype highly pathogenic avian influenza virus (hpaiv) was transmitted to both the internal contents and shells of eggs laid by white leghorn hens experimentally infected with the virus. seven of eight hpaiv-infected hens laid eggs until 4 days postinoculation (dpi). the mean number of eggs laid per head daily decreased significantly from 0.58 before inoculation to 0.18 after viral inoculation. the virus was detected in the eggs laid by three of the seven hens. viral ...	2016	27309286
characterization of clade 2.3.4.4 h5n8 highly pathogenic avian influenza viruses from wild birds possessing atypical hemagglutinin polybasic cleavage sites.	since 2014, clade 2.3.4.4 h5 subtype highly pathogenic avian influenza viruses (hpaivs) have been distributed worldwide. these viruses, which were reported to be highly virulent in chickens by intravenous inoculation, have a consensus hpai motif plrerrrkr at the ha cleavage site. however, two-clade 2.3.4.4 h5n8 viruses which we isolated from wild migratory birds in late 2014 in japan possessed atypical ha cleavage sequences. a swan isolate, tottori/c6, had a novel polybasic cleavage sequence, pl ...	2017	27738904
efficacy of a recombinant turkey herpesvirus ai (h5) vaccine in preventing transmission of heterologous highly pathogenic h5n8 clade 2.3.4.4b challenge virus in commercial broilers and layer pullets.	outbreaks caused by the highly pathogenic avian influenza virus (hpaiv) h5n8 subtype clade 2.3.4.4 were first reported in 2014 in south korea then spread very rapidly in asia, to europe, and for the first time, to north america. efficacy of a recombinant hvt-ai (h5) vaccine (rhvt-h5) to provide clinical protection as well as to significantly reduce the shedding of an h5n8 challenge virus has already been demonstrated in spf chickens. the aim of our studies was to test the efficacy of the same rh ...	2018	30584541
highly pathogenic avian influenza subtype h5nx clade 2.3.4.4 outbreaks in dutch poultry farms, 2014-2018: clinical signs and mortality.	in recent years, different subtypes of highly pathogenic avian influenza (hpai) viruses caused outbreaks in several poultry types worldwide. early detection of hpai virus infection is crucial to reduce virus spread. previously, the use of a mortality ratio threshold to expedite notification of suspicion in layer farms was proposed. the purpose of this study was to describe the clinical signs reported in the early stages of hpai h5n8 and h5n6 outbreaks on chicken and pekin duck farms between 2014 ...	2020	32418364