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media optimization for laccase production by trichoderma harzianum zf-2 using response surface methodology.trichoderma harzianum zf-2 producing laccase was isolated from decaying samples from shandong, china, and showed dye decolorization activities. the objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. the interactions between different fermentation parameters for laccase production were characterized using a plackett-burman design and the response surface methodology. the different media components were initially optimized using t ...201324043124
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